Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007507

Physical Location & Seq

Genomic Coordinates Reverse (-)

27447751 .. 27451270

Gene Structure 3520 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1182000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGAAGAATTTCCACTGGTTTAAGCAGATTTCCAGCAATGGCAAGCCTGAGAGGAGTCTCTCGCTGGGTGAGTACAATCGTGCAGTCTCATGGTCCAAGTACTTGGTGTCGTCAGGGGCCGAGATTAAAGGGGAAGGAGAGGAGGAGTGGAGTGCGGACATGTCACAGTTGTACATTGGGTGTAAATTTGCTTCCGGAAGGCATAGCAGGATTTACAGAGGGGTTTATAAGGAGAGGGATGTGGCGATTAAGCTGATAAGCCAGCCTGAGGAGGATGAAGGGTTGGCTGTTTTGCTTGAGAAGCAGTTCACTTCTGAGGTTGCTTTGCTTTTTCGATTGCACCATCCTAATATCATCACTTTTGTTGCAGCTTGTAAGAAACCTCCTGTGTTCTGCATTATCACCGAGTATTTAGCTGGGGGATCGTTAAGGAAGTATCTTCATCAGCAGGAGCCACATTCTGTTCCGCTTAGCCTAGTTATAAAATTAGCCCTCAACATTGCACGCGGGATGCAATATCTCCATTCTCAAAGTATACTTCATAGGGATCTCAAGTCAGAAAATTTATTGCTAGGGGAAGATATGTCTGTAAAGGTTGCGGATTTTGGTATTTCATGCCTAGAATCACAGTGCGGCAGCGCTAAGGGATTCACAGGTACTTACCGCTGGATGGCACCCGAAATGATCAAAGAGAAACACCATACAAAGAAAGTCGATGTTTACAGTTTTGGAATAGTTCTGTGGGAGCTTTTAACGGCATTGACACCATTTGACAACATGACTCCTGAACAGGCTGCGTTTGCAGTATCGCAGAAGAATGCAAGACCCCCTTTGCCGTCCACATGCCCGGTGCCATTCAGTCGCCTCATTAGCCGATGCTGGTCAAGCCATCCAGATAAGCGACCTCATTTCGATGAGATAGTGCACATTTTGGAAGGTTATGCCGAGTCCCTTGAGCAGGACCCAGATTTCTTCTCATCTTACAAGCCGCCCCCAGATCACGCCCTATTGCGATGCTTTCCAAAATGGAAGGGTCGCCGTGGTTCTGCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

352

Amino Acids

39.77

Weight (kDa)

8.5

Isoelectric Point (pI)

49.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	61 - 311	6.8e-63	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	65 - 309	4.1e-54	Protein kinase domain
Pkinase_fungal	PF17667	169 - 247	5.1e-06	Fungal protein kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007507)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g05240 FvH4_5g05240 FvH4_5g05240
malus_domestica	MD06G1175200.v1.1 MD14G1182000.v1.1
prunus_persica	Prupe.5G174600_v2.0.a1 Prupe.5G174600_v2.0.a1 Prupe.5G174600_v2.0.a1 Prupe.5G174600_v2.0.a1 Prupe.5G174600_v2.0.a1 Prupe.5G174600_v2.0.a1
pyrus_communis	pycom06g15600 pycom14g15100
rosa_chinensis	RchiOBHm_Chr7g0193661
rosa_laevigata	RLG00000004253
rosa_multiflora	Rmu_sc0001436.1_g000007
rosa_roxburghii	Rroxscaffold_3G00261930
rosa_rugosa	Rorug07G0011200 Rorug07G0011300 Rorug07G0011300
rosa_samantha	Rh7AG137900 Rh7BG138100 Rh7CG141800 Rh7DG139200
rosa_wichuraiana	Rw7G011740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	228, 482
AccB1I	GGYRCC	2 cut(s)	673, 850
AccI	GTMKAC	1 cut(s)	535
AccII	CGCG	1 cut(s)	507
AccIII	TCCGGA	1 cut(s)	194
AciI	CCGC	7 cut(s)	155, 467, 507, 599, 633, 664, 989
AclWI	GGATC	2 cut(s)	430, 555
AcsI	RAATTY	3 cut(s)	7, 185, 562
AfaI	GTAC	4 cut(s)	74, 101, 173, 658
AfeI	AGCGCT	1 cut(s)	640
AfiI	CCNNNNNNNGG	3 cut(s)	268, 670, 958
AflIII	ACRYGT	1 cut(s)	159
AluBI	AGCT	4 cut(s)	253, 371, 416, 748
AluI	AGCT	4 cut(s)	253, 371, 416, 748
Alw21I	GWGCWC	1 cut(s)	927
Alw26I	GTCTC	2 cut(s)	62, 91
Alw44I	GTGCAC	1 cut(s)	923
AlwI	GGATC	2 cut(s)	430, 555
Aor13HI	TCCGGA	1 cut(s)	194
Aor51HI	AGCGCT	1 cut(s)	640
AoxI	GGCC	1 cut(s)	117
ApaLI	GTGCAC	1 cut(s)	923
ApeKI	GCWGC	3 cut(s)	368, 636, 794
ApoI	RAATTY	3 cut(s)	7, 185, 562
Asp700I	GAANNNNTTC	2 cut(s)	8, 305
AspLEI	GCGC	1 cut(s)	641
AspS9I	GGNCC	3 cut(s)	93, 117, 961
AsuC2I	CCSGG	1 cut(s)	848
AsuHPI	GGTGA	2 cut(s)	80, 394
AvaII	GGWCC	2 cut(s)	93, 961
AxyI	CCTNAGG	1 cut(s)	267
BaeGI	GKGCMC	1 cut(s)	927
BanI	GGYRCC	2 cut(s)	673, 850
BarI	GAAGNNNNNNTAC	2 cut(s)	175, 207
Bbv12I	GWGCWC	1 cut(s)	927
BbvI	GCAGC	3 cut(s)	380, 648, 781
BccI	CCATC	3 cut(s)	351, 664, 897
BceAI	ACGGC	3 cut(s)	771, 820, 1023
BclI	TGATCA	1 cut(s)	684
BcnI	CCSGG	1 cut(s)	848
BcoDI	GTCTC	2 cut(s)	62, 91
BfaI	CTAG	3 cut(s)	476, 572, 620
BfoI	RGCGCY	1 cut(s)	642
BisI	GCNGC	5 cut(s)	369, 634, 637, 795, 989
BlpI	GCTNAGC	1 cut(s)	470
BlsI	GCNGC	5 cut(s)	370, 635, 638, 796, 990
BmcAI	AGTACT	1 cut(s)	101
Bme1390I	CCNGG	1 cut(s)	848
Bme18I	GGWCC	2 cut(s)	93, 961
BmgT120I	GGNCC	3 cut(s)	93, 117, 961
BmiI	GGNNCC	5 cut(s)	118, 453, 675, 852, 963
BmrFI	CCNGG	1 cut(s)	848
BmsI	GCATC	3 cut(s)	501, 866, 1004
Bpu10I	CCTNAGC	1 cut(s)	642
Bpu1102I	GCTNAGC	1 cut(s)	470
BpuEI	CTTGAG	3 cut(s)	317, 536, 974
BpuMI	CCSGG	1 cut(s)	848
BsaJI	CCNNGG	1 cut(s)	1039
BsaWI	WCCGGW	1 cut(s)	194
Bsc4I	CCNNNNNNNGG	3 cut(s)	268, 670, 958
Bse1I	ACTGG	1 cut(s)	20
Bse21I	CCTNAGG	1 cut(s)	267
Bse3DI	GCAATG	2 cut(s)	43, 498
BseAI	TCCGGA	1 cut(s)	194
BseDI	CCNNGG	1 cut(s)	1039
BseGI	GGATG	6 cut(s)	244, 280, 343, 516, 675, 889
BseLI	CCNNNNNNNGG	3 cut(s)	268, 670, 958
BseMI	GCAATG	2 cut(s)	43, 498
BseMII	CTCAG	3 cut(s)	39, 258, 306
BseNI	ACTGG	1 cut(s)	20
BseRI	GAGGAG	4 cut(s)	67, 155, 158, 284
BseSI	GKGCMC	1 cut(s)	927
BseXI	GCAGC	3 cut(s)	380, 648, 781
BseYI	CCCAGC	2 cut(s)	64, 416
BsgI	GTGCAG	1 cut(s)	102
Bsh1236I	CGCG	1 cut(s)	507
BshFI	GGCC	1 cut(s)	119
BshNI	GGYRCC	2 cut(s)	673, 850
BsiHKAI	GWGCWC	1 cut(s)	927
BsiSI	CCGG	2 cut(s)	195, 848
BslFI	GGGAC	1 cut(s)	934
BslI	CCNNNNNNNGG	3 cut(s)	268, 670, 958
BsmAI	GTCTC	2 cut(s)	62, 91
BsmFI	GGGAC	1 cut(s)	934
BsmI	GAATGC	1 cut(s)	823
BsnI	GGCC	1 cut(s)	119
Bsp1286I	GDGCHC	1 cut(s)	927
Bsp13I	TCCGGA	1 cut(s)	194
Bsp1407I	TGTACA	1 cut(s)	171
Bsp143I	GATC	4 cut(s)	422, 547, 684, 997
Bsp1720I	GCTNAGC	1 cut(s)	470
BspACI	CCGC	7 cut(s)	155, 467, 507, 599, 633, 664, 989
BspANI	GGCC	1 cut(s)	119
BspCNI	CTCAG	3 cut(s)	40, 259, 307
BspEI	TCCGGA	1 cut(s)	194
BspFNI	CGCG	1 cut(s)	507
BspLI	GGNNCC	5 cut(s)	118, 453, 675, 852, 963
BspPI	GGATC	2 cut(s)	430, 555
BspT107I	GGYRCC	2 cut(s)	673, 850
BsrDI	GCAATG	2 cut(s)	43, 498
BsrGI	TGTACA	1 cut(s)	171
BsrI	ACTGG	1 cut(s)	20
BssECI	CCNNGG	1 cut(s)	1039
BssMI	GATC	4 cut(s)	422, 547, 684, 997
BssNAI	GTATAC	1 cut(s)	536
Bst1107I	GTATAC	1 cut(s)	536
Bst4CI	ACNGT	3 cut(s)	168, 630, 725
BstAUI	TGTACA	1 cut(s)	171
BstC8I	GCNNGC	3 cut(s)	44, 263, 505
BstDEI	CTNAG	5 cut(s)	48, 267, 315, 470, 642
BstDSI	CCRYGG	1 cut(s)	1039
BstENI	CCTNNNNNAGG	1 cut(s)	956
BstF5I	GGATG	6 cut(s)	244, 280, 343, 516, 675, 889
BstFNI	CGCG	1 cut(s)	507
BstH2I	RGCGCY	1 cut(s)	642
BstHHI	GCGC	1 cut(s)	641
BstKTI	GATC	4 cut(s)	425, 550, 687, 1000
BstMAI	GTCTC	2 cut(s)	62, 91
BstMBI	GATC	4 cut(s)	422, 547, 684, 997
BstMWI	GCNNNNNNNGC	4 cut(s)	301, 800, 870, 885
BstNSI	RCATGY	2 cut(s)	163, 846
BstSCI	CCNGG	1 cut(s)	846
BstSLI	GKGCMC	1 cut(s)	927
BstUI	CGCG	1 cut(s)	507
BstV1I	GCAGC	3 cut(s)	380, 648, 781
BstX2I	RGATCY	1 cut(s)	547
BstXI	CCANNNNNNTGG	1 cut(s)	103
BstYI	RGATCY	1 cut(s)	547
BstZ17I	GTATAC	1 cut(s)	536
Bsu36I	CCTNAGG	1 cut(s)	267
BsuRI	GGCC	1 cut(s)	119
BtgI	CCRYGG	1 cut(s)	1039
BtgZI	GCGATG	1 cut(s)	1027
BtsCI	GGATG	6 cut(s)	244, 280, 343, 516, 675, 889
BtsIMutI	CAGTG	2 cut(s)	13, 635
Cac8I	GCNNGC	3 cut(s)	44, 263, 505
CfoI	GCGC	1 cut(s)	641
Cfr13I	GGNCC	3 cut(s)	93, 117, 961
Csp6I	GTAC	4 cut(s)	73, 100, 172, 657
CviAII	CATG	5 cut(s)	90, 160, 615, 778, 843
CviQI	GTAC	4 cut(s)	73, 100, 172, 657
DdeI	CTNAG	5 cut(s)	48, 267, 315, 470, 642
DpnI	GATC	4 cut(s)	424, 549, 686, 999
DpnII	GATC	4 cut(s)	422, 547, 684, 997
Eco47I	GGWCC	2 cut(s)	93, 961
Eco47III	AGCGCT	1 cut(s)	640
Eco81I	CCTNAGG	1 cut(s)	267
EcoNI	CCTNNNNNAGG	1 cut(s)	956
EcoO109I	RGGNCCY	1 cut(s)	961
FaeI	CATG	5 cut(s)	93, 163, 618, 781, 846
FalI	AAGNNNNNCTT	2 cut(s)	814, 846
FaqI	GGGAC	1 cut(s)	934
FatI	CATG	5 cut(s)	89, 159, 614, 777, 842
FauI	CCCGC	1 cut(s)	500
FbaI	TGATCA	1 cut(s)	684
FblI	GTMKAC	1 cut(s)	535
Fnu4HI	GCNGC	5 cut(s)	369, 634, 637, 795, 989
FokI	GGATG	6 cut(s)	251, 287, 330, 523, 682, 876
Fsp4HI	GCNGC	5 cut(s)	369, 634, 637, 795, 989
FspBI	CTAG	3 cut(s)	476, 572, 620
GlaI	GCGC	1 cut(s)	640
GluI	GCNGC	5 cut(s)	369, 634, 637, 795, 989
GsaI	CCCAGC	2 cut(s)	68, 420
HaeII	RGCGCY	1 cut(s)	642
HaeIII	GGCC	1 cut(s)	119
HapII	CCGG	2 cut(s)	195, 848
HhaI	GCGC	1 cut(s)	641
Hin1II	CATG	5 cut(s)	93, 163, 618, 781, 846
Hin6I	GCGC	1 cut(s)	639
HinP1I	GCGC	1 cut(s)	639
HinfI	GANTC	5 cut(s)	55, 623, 648, 781, 947
HpaII	CCGG	2 cut(s)	195, 848
HphI	GGTGA	2 cut(s)	80, 394
Hpy166II	GTNNAC	5 cut(s)	309, 536, 721, 840, 925
Hpy188I	TCNGA	2 cut(s)	316, 559
Hpy188III	TCNNGA	4 cut(s)	195, 785, 893, 1053
Hpy8I	GTNNAC	5 cut(s)	309, 536, 721, 840, 925
HpyAV	CCTTC	5 cut(s)	128, 192, 272, 929, 1024
HpyCH4III	ACNGT	3 cut(s)	168, 630, 725
HpyCH4V	TGCA	9 cut(s)	83, 340, 368, 396, 503, 514, 803, 821, 925
HpyF10VI	GCNNNNNNNGC	4 cut(s)	301, 800, 870, 885
HpyF3I	CTNAG	5 cut(s)	48, 267, 315, 470, 642
Hsp92II	CATG	5 cut(s)	93, 163, 618, 781, 846
HspAI	GCGC	1 cut(s)	639
Kpn2I	TCCGGA	1 cut(s)	194
Ksp22I	TGATCA	1 cut(s)	684
Kzo9I	GATC	4 cut(s)	422, 547, 684, 997
LmnI	GCTCC	2 cut(s)	451, 745
Lsp1109I	GCAGC	3 cut(s)	380, 648, 781
LweI	GCATC	3 cut(s)	501, 866, 1004
MaeI	CTAG	3 cut(s)	476, 572, 620
MaeIII	GTNAC	1 cut(s)	162
MalI	GATC	4 cut(s)	424, 549, 686, 999
MboI	GATC	4 cut(s)	422, 547, 684, 997
MboII	GAAGA	5 cut(s)	16, 431, 590, 826, 964
MflI	RGATCY	1 cut(s)	547
MhlI	GDGCHC	1 cut(s)	927
MluCI	AATT	4 cut(s)	7, 185, 485, 562
MlyI	GAGTC	3 cut(s)	64, 775, 956
MroI	TCCGGA	1 cut(s)	194
MroXI	GAANNNNTTC	2 cut(s)	8, 305
MseI	TTAA	5 cut(s)	21, 126, 249, 428, 752
MslI	CAYNNNNRTG	1 cut(s)	912
MspA1I	CMGCKG	1 cut(s)	666
MspI	CCGG	2 cut(s)	195, 848
MspR9I	CCNGG	1 cut(s)	848
Mva1269I	GAATGC	1 cut(s)	823
MvnI	CGCG	1 cut(s)	507
MwoI	GCNNNNNNNGC	4 cut(s)	301, 800, 870, 885
NciI	CCSGG	1 cut(s)	848
NdeII	GATC	4 cut(s)	422, 547, 684, 997
NlaIII	CATG	5 cut(s)	93, 163, 618, 781, 846
NlaIV	GGNNCC	5 cut(s)	118, 453, 675, 852, 963
NmeAIII	GCCGAG	2 cut(s)	145, 970
NmuCI	GTSAC	1 cut(s)	162
NspI	RCATGY	2 cut(s)	163, 846
PciI	ACATGT	1 cut(s)	159
PctI	GAATGC	1 cut(s)	823
PdmI	GAANNNNTTC	2 cut(s)	8, 305
PfeI	GAWTC	2 cut(s)	623, 648
PkrI	GCNGC	5 cut(s)	370, 635, 638, 796, 990
PleI	GAGTC	3 cut(s)	63, 775, 955
PpsI	GAGTC	3 cut(s)	63, 775, 955
PpuMI	RGGWCCY	1 cut(s)	961
PscI	ACATGT	1 cut(s)	159
PsiI	TTATAA	2 cut(s)	228, 482
Psp5II	RGGWCCY	1 cut(s)	961
PspFI	CCCAGC	2 cut(s)	64, 416
PspN4I	GGNNCC	5 cut(s)	118, 453, 675, 852, 963
PspPI	GGNCC	3 cut(s)	93, 117, 961
PspPPI	RGGWCCY	1 cut(s)	961
PsuI	RGATCY	1 cut(s)	547
RsaI	GTAC	4 cut(s)	74, 101, 173, 658
RsaNI	GTAC	4 cut(s)	73, 100, 172, 657
RseI	CAYNNNNRTG	1 cut(s)	912
SaqAI	TTAA	5 cut(s)	21, 126, 249, 428, 752
SatI	GCNGC	5 cut(s)	369, 634, 637, 795, 989
Sau3AI	GATC	4 cut(s)	422, 547, 684, 997
Sau96I	GGNCC	3 cut(s)	93, 117, 961
ScaI	AGTACT	1 cut(s)	101
SchI	GAGTC	3 cut(s)	64, 775, 956
ScrFI	CCNGG	1 cut(s)	848
SduI	GDGCHC	1 cut(s)	927
SfaNI	GCATC	3 cut(s)	501, 866, 1004
SinI	GGWCC	2 cut(s)	93, 961
SmiMI	CAYNNNNRTG	1 cut(s)	912
SmlI	CTYRAG	3 cut(s)	296, 551, 953
SmoI	CTYRAG	3 cut(s)	296, 551, 953
Sse9I	AATT	4 cut(s)	7, 185, 485, 562
SsiI	CCGC	7 cut(s)	155, 467, 507, 599, 633, 664, 989
SspMI	CTAG	3 cut(s)	476, 572, 620
StyD4I	CCNGG	1 cut(s)	846
TaaI	ACNGT	3 cut(s)	168, 630, 725
TaqI	TCGA	3 cut(s)	334, 714, 912
TasI	AATT	4 cut(s)	7, 185, 485, 562
TatI	WGTACW	3 cut(s)	72, 99, 171
TauI	GCSGC	2 cut(s)	636, 991
TfiI	GAWTC	2 cut(s)	623, 648
Tru1I	TTAA	5 cut(s)	21, 126, 249, 428, 752
Tru9I	TTAA	5 cut(s)	21, 126, 249, 428, 752
TscAI	CASTG	2 cut(s)	20, 635
TseFI	GTSAC	1 cut(s)	162
TseI	GCWGC	3 cut(s)	368, 636, 794
Tsp45I	GTSAC	1 cut(s)	162
TspDTI	ATGAA	5 cut(s)	17, 291, 431, 530, 603
TspRI	CASTG	2 cut(s)	20, 635
VneI	GTGCAC	1 cut(s)	923
VpaK11BI	GGWCC	2 cut(s)	93, 961
XagI	CCTNNNNNAGG	1 cut(s)	956
XapI	RAATTY	3 cut(s)	7, 185, 562
XceI	RCATGY	2 cut(s)	163, 846
XmiI	GTMKAC	1 cut(s)	535
XmnI	GAANNNNTTC	2 cut(s)	8, 305
XspI	CTAG	3 cut(s)	476, 572, 620
ZrmI	AGTACT	1 cut(s)	101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.