Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005764

Physical Location & Seq

Genomic Coordinates Forward (+)

28038549 .. 28041908

Gene Structure 3360 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1188700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1857 bp

ATGGGTAGTGAAAGGCTTGGAGAGATGTTGAGTATAAGTGAGGAGCTTATCAAGAAAAGCTGTTATCTTTCCTTCCAGGCGCACAAATTCCCAGATGATCTGTTTCGTGTCGAAAAATTTGCTGCAGGATCATCAGATGTTGTTTTCAGCTTCCCAGGATCTTGGTCTGTGGAGAGTTGGGCTTCTGTAGAAAGTGCTTTTGGGGACAAAAAGATCCAACTTTCACTCTTTCCTTCCATGAAAAGCGTAGGTTATTATGATGAAGCTGCAGGACCTGATGAGCACCAAGATTTTGCAACTGCAAGTTTCAACCAAGCCTTTCTGCAAAAGTTTGAACATCTATTGCAGAATTCCCAGCTTAAAAATAAGGTGCAAGAGGCTATCAATGAAAAGAAGAGAATAGTATTTACCGGCCACTCTACCGGCGGAGCAACTGCTGTGCTGACCACAATCTGGTGCTTGGAAAATTATCCGAAACAAAGCAATGGAACATTTTTCTGTGTGACATTCGGATCTCCCCTTATTGGTAACCATATTATTTCCCATGCCGTTAGGAGAGAAAATTGGTCTCAATACTTCATACATTTTGTCAAGAGATATGACATTGTCCCTAGAATTTTGCTTGCTCCGCTCTCGTCGATTCAACATGAATTCGAGAGGATCCTCCCCTTCTTCGATCCAAAATCCCCCGCTTACGCGAGTGAATCAATTGGATCATCCGTGGAGGCCTTCCGTTTGTTTACAAATGTGATGAGAAATGCAGCATCTCTTACAACTCATGCTGCCTCCAAACTGCAGGGGAGTACCAACCTATTACTAGAAACTGTAAAAAAGTTCGTTAAGCTAAGCCCATACAAACCTTTTGGAACTTACATTTTTTGTACCGGGAACGGGAATTTGGTTCTTATGAAAAACCCTGAAGCTGTGCTGCAAGTCTTGTTCTACTCGTGTCAATCGAGCAGCGAAACTGAATGGGCAACGATCGTGCTTAAATGCCTAAAGGAGCATTTGGCCTACGAGAATGAGCTTGAAGGCAGCTTGGACATGCAGAGTGTGACTTGTGATGATAACCTAGAAACGCTCCCCTTAGCCTCAGATGGTTTCTTTGATGACCTTGGCTTGAGCGAAAAGGCTAGATTATGCCTTCGTGCAGCGGGAGAATTCGAGAAGCAAAAACGAAGAAACCAGGAAAAAGTAAGTTCAAAAATGGAGATGATCAGGAAACATTTGAAAGTAATCGAAGAGTACCGAGCCTTATGCGAGCACACTGTCGGATATTACGACGCCTTCAAAATTCATAAAGACAAGACAGATTTCAAGGCTAATTTGAGCAGGATTGAGCTAACAGGTATATGGGATGAGATCATTGAGATGTTGAAAAGATACGACCTTCCCGATGAACTCGAGGCTACGAAGGAGTGGATAAATCTAGGAACCAGGTTTCGCCGCCTTGTCGAGCCTCTCGATGTTGCTAACTACTACAGGCACTCCAAGGACGAAGACACAGGACCCTATATGAAGAAAGGCAGGCCAAAGCGATACAAATGCATGCAAAGATGGCTCGAGCACGAACAGAAACTGCAATCTGGTTCATGCGGGGAGTCCTGCTTCTGGGCAGATGTGGAGGAGTTGCACAAGCTTTCAGGAAACTTCGCGGCGGTGTACGGCGAAAGGGAGAGGGTTTTGAGAGTTCAGGAGCAAGTAGGAAAATGGATTCAGGACGGGTTGATCGGTACGGATGTGTTGCTGAAGTCGTCCACCTTCGAGAAGTGGTGGAGCAAGCTCCCTACTGACCTCAAAACAGAAACGATTTCGAGACGCATGAATGGTTTAGGAAATGTGCAAGCGTGGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000302 GO:0000304 GO:0001666 GO:0002376 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007584 GO:0008150 GO:0008219 GO:0009605 GO:0009607 GO:0009626 GO:0009627 GO:0009628 GO:0009653 GO:0009814 GO:0009987 GO:0009991 GO:0010035 GO:0010310 GO:0010618 GO:0010941 GO:0010942 GO:0012501 GO:0016298 GO:0016787 GO:0016788 GO:0019222 GO:0031323 GO:0031667 GO:0032502 GO:0033554 GO:0034050 GO:0036293 GO:0042221 GO:0042493 GO:0042802 GO:0042803 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045087 GO:0046983 GO:0048518 GO:0048522 GO:0048646 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051193 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070482 GO:0098542 GO:1901700 GO:2000377

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Lipase_3

Protein Analysis

619

Amino Acids

70.72

Weight (kDa)

6.63

Isoelectric Point (pI)

43.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lipase_3	PF01764	99 - 207	1.9e-20	Lipase (class 3)
EDS1_EP	PF18117	412 - 602	4.2e-56	Enhanced disease susceptibility 1 protein EP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48080 AT3G48090 AT3G48090
fragaria_vesca	FvH4_5g16230 FvH4_5g16230
malus_domestica	MD06G1182600.v1.1 MD06G1182800.v1.1 MD14G1188600.v1.1 MD14G1188700.v1.1
prunus_persica	Prupe.5G180700_v2.0.a1 Prupe.5G180900_v2.0.a1 Prupe.5G181000_v2.0.a1
pyrus_communis	pycom06g16150 pycom06g16180 pycom14g15630 pycom14g15640
rosa_chinensis	RchiOBHm_Chr2g0170571 RchiOBHm_Chr7g0179871
rosa_laevigata	RLG00000005316
rosa_multiflora	Rmu_sc0012773.1_g000001 Rmu_sc0016092.1_g000001
rosa_roxburghii	Rroxscaffold_3G00273380
rosa_rugosa	Rorug06G0428800
rosa_samantha	Rh7AG028900 Rh7BG028000 Rh7CG029800 Rh7DG029200
rosa_wichuraiana	Rw7G002310

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	453
AccBSI	CCGCTC	1 cut(s)	631
AccII	CGCG	2 cut(s)	698, 1655
AciI	CCGC	8 cut(s)	426, 629, 690, 1154, 1447, 1596, 1655, 1658
AclWI	GGATC	8 cut(s)	136, 166, 208, 520, 655, 668, 671, 721
AcoI	YGGCCR	1 cut(s)	412
AcsI	RAATTY	8 cut(s)	86, 116, 349, 615, 650, 895, 1160, 1293
AcuI	CTGAAG	2 cut(s)	939, 1769
AcyI	GRCGYC	1 cut(s)	1284
AfaI	GTAC	5 cut(s)	805, 883, 1247, 1664, 1735
AfiI	CCNNNNNNNGG	4 cut(s)	453, 524, 891, 1611
AgsI	TTSAA	9 cut(s)	310, 335, 644, 1031, 1203, 1231, 1291, 1318, 1378
AjnI	CCWGG	4 cut(s)	75, 154, 1185, 1436
AjuI	GAANNNNNNNTTGG	4 cut(s)	183, 215, 881, 913
Alw21I	GWGCWC	3 cut(s)	285, 1266, 1569
Alw26I	GTCTC	2 cut(s)	573, 1810
AlwI	GGATC	8 cut(s)	136, 166, 208, 520, 655, 668, 671, 721
AlwNI	CAGNNNCTG	2 cut(s)	275, 1579
Ama87I	CYCGRG	2 cut(s)	1403, 1562
AoxI	GGCC	4 cut(s)	412, 726, 1011, 1529
ApeKI	GCWGC	8 cut(s)	122, 266, 761, 782, 928, 960, 1035, 1151
ApoI	RAATTY	8 cut(s)	86, 116, 349, 615, 650, 895, 1160, 1293
AspLEI	GCGC	1 cut(s)	82
AspS9I	GGNCC	2 cut(s)	272, 1508
AsuC2I	CCSGG	1 cut(s)	886
AvaI	CYCGRG	2 cut(s)	1403, 1562
AvaII	GGWCC	2 cut(s)	272, 1508
BaeI	ACNNNNGTAYC	2 cut(s)	1725, 1758
BamHI	GGATCC	1 cut(s)	660
BauI	CACGAG	1 cut(s)	946
BbsI	GAAGAC	1 cut(s)	1506
Bbv12I	GWGCWC	3 cut(s)	285, 1266, 1569
BbvI	GCAGC	8 cut(s)	109, 253, 769, 773, 915, 972, 1047, 1163
BccI	CCATC	2 cut(s)	1091, 1551
BceAI	ACGGC	2 cut(s)	533, 1681
BcgI	CGANNNNNNTGC	6 cut(s)	101, 135, 1239, 1273, 1527, 1561
BciT130I	CCWGG	4 cut(s)	77, 156, 1187, 1438
BclI	TGATCA	1 cut(s)	1215
BcnI	CCSGG	1 cut(s)	886
BcoDI	GTCTC	2 cut(s)	573, 1810
BfaI	CTAG	5 cut(s)	612, 818, 1073, 1134, 1430
BfmI	CTRYAG	5 cut(s)	123, 186, 267, 794, 1480
BlpI	GCTNAGC	1 cut(s)	845
Bme1390I	CCNGG	5 cut(s)	77, 156, 886, 1187, 1438
Bme18I	GGWCC	2 cut(s)	272, 1508
BmeT110I	CYCGRG	2 cut(s)	1403, 1562
BmgT120I	GGNCC	2 cut(s)	272, 1508
BmiI	GGNNCC	3 cut(s)	662, 1435, 1510
BmrFI	CCNGG	5 cut(s)	77, 156, 886, 1187, 1438
BmsI	GCATC	1 cut(s)	773
BpiI	GAAGAC	1 cut(s)	1506
Bpu10I	CCTNAGC	1 cut(s)	1087
Bpu1102I	GCTNAGC	1 cut(s)	845
BpuEI	CTTGAG	1 cut(s)	1141
BpuMI	CCSGG	1 cut(s)	886
BsaHI	GRCGYC	1 cut(s)	1284
BsaI	GGTCTC	1 cut(s)	573
BsaJI	CCNNGG	4 cut(s)	154, 720, 1114, 1491
BsaXI	ACNNNNNCTCC	2 cut(s)	1472, 1502
Bsc4I	CCNNNNNNNGG	4 cut(s)	453, 524, 891, 1611
Bse118I	RCCGGY	2 cut(s)	410, 422
Bse3DI	GCAATG	1 cut(s)	490
BseBI	CCWGG	4 cut(s)	77, 156, 1187, 1438
BseDI	CCNNGG	4 cut(s)	154, 720, 1114, 1491
BseGI	GGATG	3 cut(s)	716, 1363, 1744
BseLI	CCNNNNNNNGG	4 cut(s)	453, 524, 891, 1611
BseMI	GCAATG	1 cut(s)	490
BseMII	CTCAG	1 cut(s)	1107
BseRI	GAGGAG	2 cut(s)	56, 1640
BseXI	GCAGC	8 cut(s)	109, 253, 769, 773, 915, 972, 1047, 1163
BseYI	CCCAGC	1 cut(s)	354
BsgI	GTGCAG	1 cut(s)	1170
Bsh1236I	CGCG	2 cut(s)	698, 1655
Bsh1285I	CGRYCG	1 cut(s)	984
BshFI	GGCC	4 cut(s)	414, 728, 1013, 1531
BsiEI	CGRYCG	1 cut(s)	984
BsiHKAI	GWGCWC	3 cut(s)	285, 1266, 1569
BsiHKCI	CYCGRG	2 cut(s)	1403, 1562
BsiSI	CCGG	3 cut(s)	411, 423, 885
BslFI	GGGAC	2 cut(s)	218, 593
BslI	CCNNNNNNNGG	4 cut(s)	453, 524, 891, 1611
BsmAI	GTCTC	2 cut(s)	573, 1810
BsmBI	CGTCTC	1 cut(s)	1810
BsmFI	GGGAC	2 cut(s)	218, 593
BsnI	GGCC	4 cut(s)	414, 728, 1013, 1531
Bso31I	GGTCTC	1 cut(s)	573
BsoBI	CYCGRG	2 cut(s)	1403, 1562
Bsp1286I	GDGCHC	3 cut(s)	285, 1266, 1569
Bsp1720I	GCTNAGC	1 cut(s)	845
BspACI	CCGC	8 cut(s)	426, 629, 690, 1154, 1447, 1596, 1655, 1658
BspANI	GGCC	4 cut(s)	414, 728, 1013, 1531
BspCNI	CTCAG	1 cut(s)	1106
BspFNI	CGCG	2 cut(s)	698, 1655
BspLI	GGNNCC	3 cut(s)	662, 1435, 1510
BspMAI	CTGCAG	3 cut(s)	127, 271, 798
BspPI	GGATC	8 cut(s)	136, 166, 208, 520, 655, 668, 671, 721
BspTNI	GGTCTC	1 cut(s)	573
BsrBI	CCGCTC	1 cut(s)	631
BsrDI	GCAATG	1 cut(s)	490
BsrFI	RCCGGY	2 cut(s)	410, 422
BssAI	RCCGGY	2 cut(s)	410, 422
BssECI	CCNNGG	4 cut(s)	154, 720, 1114, 1491
BssNI	GRCGYC	1 cut(s)	1284
BssSI	CACGAG	1 cut(s)	946
BssT1I	CCWWGG	2 cut(s)	1114, 1491
Bst2BI	CACGAG	1 cut(s)	946
Bst2UI	CCWGG	4 cut(s)	77, 156, 1187, 1438
Bst4CI	ACNGT	2 cut(s)	826, 1270
Bst6I	CTCTTC	2 cut(s)	389, 1236
BstACI	GRCGYC	1 cut(s)	1284
BstC8I	GCNNGC	6 cut(s)	624, 1262, 1529, 1550, 1781, 1845
BstDEI	CTNAG	3 cut(s)	845, 1087, 1093
BstDSI	CCRYGG	1 cut(s)	720
BstEII	GGTNACC	1 cut(s)	527
BstF5I	GGATG	3 cut(s)	716, 1363, 1744
BstFNI	CGCG	2 cut(s)	698, 1655
BstHHI	GCGC	1 cut(s)	82
BstMAI	GTCTC	2 cut(s)	573, 1810
BstMCI	CGRYCG	1 cut(s)	984
BstMWI	GCNNNNNNNGC	3 cut(s)	628, 1558, 1849
BstNI	CCWGG	4 cut(s)	77, 156, 1187, 1438
BstNSI	RCATGY	2 cut(s)	1048, 1552
BstPI	GGTNACC	1 cut(s)	527
BstSCI	CCNGG	5 cut(s)	75, 154, 884, 1185, 1436
BstSFI	CTRYAG	5 cut(s)	123, 186, 267, 794, 1480
BstUI	CGCG	2 cut(s)	698, 1655
BstV1I	GCAGC	8 cut(s)	109, 253, 769, 773, 915, 972, 1047, 1163
BstV2I	GAAGAC	1 cut(s)	1506
BstX2I	RGATCY	4 cut(s)	158, 213, 512, 660
BstXI	CCANNNNNNTGG	1 cut(s)	162
BstYI	RGATCY	4 cut(s)	158, 213, 512, 660
BsuRI	GGCC	4 cut(s)	414, 728, 1013, 1531
BtgI	CCRYGG	1 cut(s)	720
BtsCI	GGATG	3 cut(s)	716, 1363, 1744
BtsIMutI	CAGTG	1 cut(s)	1266
Cac8I	GCNNGC	6 cut(s)	624, 1262, 1529, 1550, 1781, 1845
CaiI	CAGNNNCTG	2 cut(s)	275, 1579
CfoI	GCGC	1 cut(s)	82
Cfr10I	RCCGGY	2 cut(s)	410, 422
Cfr13I	GGNCC	2 cut(s)	272, 1508
CseI	GACGC	2 cut(s)	1292, 1827
CsiI	ACCWGGT	1 cut(s)	1436
Csp6I	GTAC	5 cut(s)	804, 882, 1246, 1663, 1734
CviAII	CATG	8 cut(s)	238, 545, 647, 779, 1045, 1549, 1593, 1822
CviQI	GTAC	5 cut(s)	804, 882, 1246, 1663, 1734
DdeI	CTNAG	3 cut(s)	845, 1087, 1093
EaeI	YGGCCR	1 cut(s)	412
Eam1104I	CTCTTC	2 cut(s)	389, 1236
EarI	CTCTTC	2 cut(s)	389, 1236
EciI	GGCGGA	1 cut(s)	441
Eco130I	CCWWGG	2 cut(s)	1114, 1491
Eco147I	AGGCCT	1 cut(s)	728
Eco31I	GGTCTC	1 cut(s)	573
Eco47I	GGWCC	2 cut(s)	272, 1508
Eco57I	CTGAAG	2 cut(s)	939, 1769
Eco88I	CYCGRG	2 cut(s)	1403, 1562
Eco91I	GGTNACC	1 cut(s)	527
EcoO109I	RGGNCCY	2 cut(s)	272, 1508
EcoO65I	GGTNACC	1 cut(s)	527
EcoRI	GAATTC	3 cut(s)	349, 650, 1160
EcoRII	CCWGG	4 cut(s)	75, 154, 1185, 1436
EcoT14I	CCWWGG	2 cut(s)	1114, 1491
EcoT22I	ATGCAT	1 cut(s)	1550
ErhI	CCWWGG	2 cut(s)	1114, 1491
Esp3I	CGTCTC	1 cut(s)	1810
FaeI	CATG	8 cut(s)	241, 548, 650, 782, 1048, 1552, 1596, 1825
FaqI	GGGAC	2 cut(s)	218, 593
FatI	CATG	8 cut(s)	237, 544, 646, 778, 1044, 1548, 1592, 1821
FauI	CCCGC	3 cut(s)	697, 1147, 1589
FbaI	TGATCA	1 cut(s)	1215
FokI	GGATG	3 cut(s)	703, 1370, 1751
FspBI	CTAG	5 cut(s)	612, 818, 1073, 1134, 1430
GlaI	GCGC	1 cut(s)	81
GsaI	CCCAGC	1 cut(s)	358
HaeIII	GGCC	4 cut(s)	414, 728, 1013, 1531
HapII	CCGG	3 cut(s)	411, 423, 885
HgaI	GACGC	2 cut(s)	1292, 1827
HhaI	GCGC	1 cut(s)	82
Hin1I	GRCGYC	1 cut(s)	1284
Hin1II	CATG	8 cut(s)	241, 548, 650, 782, 1048, 1552, 1596, 1825
Hin6I	GCGC	1 cut(s)	80
HinP1I	GCGC	1 cut(s)	80
HindIII	AAGCTT	1 cut(s)	1637
HinfI	GANTC	4 cut(s)	640, 704, 1601, 1714
HpaII	CCGG	3 cut(s)	411, 423, 885
Hpy166II	GTNNAC	3 cut(s)	741, 1663, 1758
Hpy188I	TCNGA	5 cut(s)	136, 474, 512, 1096, 1274
Hpy8I	GTNNAC	3 cut(s)	741, 1663, 1758
Hpy99I	CGWCG	2 cut(s)	640, 1286
HpyCH4III	ACNGT	2 cut(s)	826, 1270
HpyF10VI	GCNNNNNNNGC	3 cut(s)	628, 1558, 1849
HpyF3I	CTNAG	3 cut(s)	845, 1087, 1093
Hsp92I	GRCGYC	1 cut(s)	1284
Hsp92II	CATG	8 cut(s)	241, 548, 650, 782, 1048, 1552, 1596, 1825
HspAI	GCGC	1 cut(s)	80
Ksp22I	TGATCA	1 cut(s)	1215
LmnI	GCTCC	8 cut(s)	43, 428, 631, 1003, 1086, 1696, 1776, 1788
Lsp1109I	GCAGC	8 cut(s)	109, 253, 769, 773, 915, 972, 1047, 1163
LweI	GCATC	1 cut(s)	773
MabI	ACCWGGT	1 cut(s)	1436
MaeI	CTAG	5 cut(s)	612, 818, 1073, 1134, 1430
MaeIII	GTNAC	3 cut(s)	502, 527, 1054
MbiI	CCGCTC	1 cut(s)	631
MboII	GAAGA	6 cut(s)	406, 664, 1191, 1253, 1511, 1531
MfeI	CAATTG	1 cut(s)	708
MflI	RGATCY	4 cut(s)	158, 213, 512, 660
MhlI	GDGCHC	3 cut(s)	285, 1266, 1569
MlyI	GAGTC	1 cut(s)	1610
MmeI	TCCRAC	2 cut(s)	241, 1252
Mph1103I	ATGCAT	1 cut(s)	1550
MseI	TTAA	3 cut(s)	360, 840, 990
MslI	CAYNNNNRTG	1 cut(s)	1370
MspA1I	CMGCKG	1 cut(s)	1154
MspI	CCGG	3 cut(s)	411, 423, 885
MspR9I	CCNGG	5 cut(s)	77, 156, 886, 1187, 1438
MunI	CAATTG	1 cut(s)	708
MvaI	CCWGG	4 cut(s)	77, 156, 1187, 1438
MvnI	CGCG	2 cut(s)	698, 1655
MwoI	GCNNNNNNNGC	3 cut(s)	628, 1558, 1849
NciI	CCSGG	1 cut(s)	886
NlaIII	CATG	8 cut(s)	241, 548, 650, 782, 1048, 1552, 1596, 1825
NlaIV	GGNNCC	3 cut(s)	662, 1435, 1510
NmuCI	GTSAC	2 cut(s)	502, 1054
NsiI	ATGCAT	1 cut(s)	1550
NspI	RCATGY	2 cut(s)	1048, 1552
PaeI	GCATGC	1 cut(s)	1552
PaeR7I	CTCGAG	2 cut(s)	1403, 1562
PceI	AGGCCT	1 cut(s)	728
PcsI	WCGNNNNNNNCGW	4 cut(s)	953, 1278, 1392, 1461
PfeI	GAWTC	3 cut(s)	640, 704, 1714
PflFI	GACNNNGTC	1 cut(s)	605
PflMI	CCANNNNNTGG	1 cut(s)	453
Ple19I	CGATCG	1 cut(s)	984
PleI	GAGTC	1 cut(s)	1609
PpsI	GAGTC	1 cut(s)	1609
PpuMI	RGGWCCY	2 cut(s)	272, 1508
Psp5II	RGGWCCY	2 cut(s)	272, 1508
Psp6I	CCWGG	4 cut(s)	75, 154, 1185, 1436
PspEI	GGTNACC	1 cut(s)	527
PspFI	CCCAGC	1 cut(s)	354
PspGI	CCWGG	4 cut(s)	75, 154, 1185, 1436
PspN4I	GGNNCC	3 cut(s)	662, 1435, 1510
PspPI	GGNCC	2 cut(s)	272, 1508
PspPPI	RGGWCCY	2 cut(s)	272, 1508
PspXI	VCTCGAGB	2 cut(s)	1403, 1562
PstI	CTGCAG	3 cut(s)	127, 271, 798
PstNI	CAGNNNCTG	2 cut(s)	275, 1579
PsuI	RGATCY	4 cut(s)	158, 213, 512, 660
PsyI	GACNNNGTC	1 cut(s)	605
PvuI	CGATCG	1 cut(s)	984
RsaI	GTAC	5 cut(s)	805, 883, 1247, 1664, 1735
RsaNI	GTAC	5 cut(s)	804, 882, 1246, 1663, 1734
RseI	CAYNNNNRTG	1 cut(s)	1370
SaqAI	TTAA	3 cut(s)	360, 840, 990
Sau96I	GGNCC	2 cut(s)	272, 1508
SchI	GAGTC	1 cut(s)	1610
ScrFI	CCNGG	5 cut(s)	77, 156, 886, 1187, 1438
SduI	GDGCHC	3 cut(s)	285, 1266, 1569
SexAI	ACCWGGT	1 cut(s)	1436
SfaNI	GCATC	1 cut(s)	773
SfcI	CTRYAG	5 cut(s)	123, 186, 267, 794, 1480
Sfr274I	CTCGAG	2 cut(s)	1403, 1562
SinI	GGWCC	2 cut(s)	272, 1508
SlaI	CTCGAG	2 cut(s)	1403, 1562
SmiMI	CAYNNNNRTG	1 cut(s)	1370
SmlI	CTYRAG	3 cut(s)	1120, 1403, 1562
SmoI	CTYRAG	3 cut(s)	1120, 1403, 1562
SphI	GCATGC	1 cut(s)	1552
SseBI	AGGCCT	1 cut(s)	728
SsiI	CCGC	8 cut(s)	426, 629, 690, 1154, 1447, 1596, 1655, 1658
SspMI	CTAG	5 cut(s)	612, 818, 1073, 1134, 1430
StuI	AGGCCT	1 cut(s)	728
StyD4I	CCNGG	5 cut(s)	75, 154, 884, 1185, 1436
StyI	CCWWGG	2 cut(s)	1114, 1491
TaaI	ACNGT	2 cut(s)	826, 1270
TauI	GCSGC	2 cut(s)	1449, 1658
TfiI	GAWTC	3 cut(s)	640, 704, 1714
Tru1I	TTAA	3 cut(s)	360, 840, 990
Tru9I	TTAA	3 cut(s)	360, 840, 990
TscAI	CASTG	1 cut(s)	1273
TseFI	GTSAC	2 cut(s)	502, 1054
TseI	GCWGC	8 cut(s)	122, 266, 761, 782, 928, 960, 1035, 1151
Tsp45I	GTSAC	2 cut(s)	502, 1054
TspGWI	ACGGA	3 cut(s)	709, 722, 1751
TspRI	CASTG	1 cut(s)	1273
Tth111I	GACNNNGTC	1 cut(s)	605
Van91I	CCANNNNNTGG	1 cut(s)	453
VpaK11BI	GGWCC	2 cut(s)	272, 1508
XapI	RAATTY	8 cut(s)	86, 116, 349, 615, 650, 895, 1160, 1293
XceI	RCATGY	2 cut(s)	1048, 1552
XhoI	CTCGAG	2 cut(s)	1403, 1562
XspI	CTAG	5 cut(s)	612, 818, 1073, 1134, 1430
Zsp2I	ATGCAT	1 cut(s)	1550

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.