Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0003065

Physical Location & Seq

Genomic Coordinates Forward (+)

29360972 .. 29365597

Gene Structure 4626 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1206400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2889 bp

ATGCATCAAATTTCAGATTTTCTGCAAATGGGGGCAAACATCCAGTGCAACAGCTATTTCCCAGGATATTATGCCTCAGGGAATCATAACTTAATTGCTAATGACAGTAAAAGGAAGCATAACTTATCTACGGACCATTTCGAGTACTACAAGGAGGCACTAAAGCAGACAATGCTCAAGCAAGACACAATATTTAGGAATCAGGTTCAGGACCTTCATCGGCTTTACGGAAGACAAAGGGAATTAATGGATGAAATAAGAAGGAATGAATTCGAGAAACATCAGCAGAAGATGGAGGCTTCATGGTTGACTACTGATTTGTCTCGGAGCTCATCTGTTTGTGTTCAAAAGACACTTCACGTTCCTTCCTTGCCTTTGGTAAACCCTGCATGCAGTCAAATCTCTGTTTCTGTTGCTGAAAGTTTCCAGTCACCTTCATGTTTTGTTCGAGGGAGGGACATACAGACATGCTCTTATCCTACTCAAACTGAAGGTCGTTCAGGAGACTCTGAATCGTTGGAATCCAAATGCAACAAATTTCAGAAGAACTTCTTGGATCTCGAACTTCCAGCGGATGCATACATTGATGATGAAGGAGAAGGATTCTTAGTAGATGGAAAGGTTTCTGAAGCACCTCAGGTTTCAAGTTCTCGTTTGATAAGATTTCCGGAGGCTGTATGTAATGGTGATGCAAAACAATTTCTTGGCACTGATGGATATAGTTCTGTTTCGGAAGATGATACGTCAACTACTGCATCATTACAAAAAGGTTCAGTTGGCTTAAATTATCTGCATAAGCTTGAGAAAGGAACATCTCCTACATCAGGCCTTCAGTTTTTATCTAAGGAATATATCCAAGACACCTGGAAAAGACAGGATTTTGAAGTTTGCTCAAATGTCCTACTGCAAGAGAGAAAAAGGAAACAAGAGTTGCCGTCCACTGACGAAGCTGAGAAAAGGAAAAGAACCTTGAATTCTTTTCCTCCAGGATCTCATGCAGATAAATTATGTCCTTCATCTAGTTTATTGCATGAGGAGCTCAAGGCAGCTGAACCTCCAACCCTTCATCAGAGTAACCAGAATTCATGGAGTGGAAGAACAATTTTCGGTCTACAGAACCCCAGAAGTTATAGCCAATCTGGACCAAGCGGAGCTTCTTCCATGTGCACGCCATATGAACATATTCCTCAGGGCAAGATGGAAAATTCAGGGGCCTCATGCAGTGCAGCTATAAGAAAACCTGTACATGACTTTGCACGGTTCCCAATGGCAGTTCAAGCACTCCCATGTTTTAACACCCCCGTACCATTTGGTACTTGTTCTAAATCATCAACGGTAAGGCCTGGGATCAATGGTGACAGGCTACAGTTGAAAAATGATTTGAGATCTAGCACAAAACATGGTAGTGCCTTCTTTCTTGATGATAACTTTAGCAATGGCTCCCAGTTGGAATCCAAGAATTCACAGCCACACATCAGTTTTGATAATCTGATCCGGACCAGTGACAATTTAGTGATTGAACTGCCTGGAGTAAAAAAAGATGTGATGGATTCCGCAAATATCAAGTCTCTTAAGGATATTAACTTGAATTGTGTGCCTACTGGCTGTTCTCTGGATGCGGCAGTTTCTCAGTGTTTCCAAGCCACAACCGGATCAGAAAAACTTGAAGCTTCTTGTGAGAGCTTGCCTTGGCATAGGCGAAAGCTTAATGGCAAAACTGACAAAGGATGTGAAGATTCAACCCAGGCGGGGGCAAGTGATTTCTCAAGTAAAATAATTCGTGAGTCTACTGGCCACCCCTCCAATTCCATTCTGAACCCACCTGAAGAAGTCAAGAAAAGAAGAAAAGTGGTGGTACTTGATTTAAACGCTGATTGTGATTCAGTGCTTGATGCAGAAATAGAACTTACAGGTCAGGTAGTAGAGAATGAATTTGATAGGAAAGATTTTGGATTTCAAATTGCCCTGAACTCGTCCACAACTGGAGATGGGTTTTCACCAGCAAACTCTCTCTCAACTGGAATTCTTTTGGAGGCACCTGCTAGTCCAGAAAACAAGGAGTGTTCTCCACCAAGAGGAGAATCGGACGAAAATCAAGTTGAGACACCTTTTCTATTGCCAGGACAGGATGATCCGGAAAACAATGAGTTTTCTCCGCCGATAATACAATTTGATGAAAACCAAGTTGTCAGGAATGCAGCAGAATCTTTGGTTTCCATTTCATCATTTGACCTTCGTAGTTGTATCATAAGGACCACGGACAAGCCACTTAAAAATTCATGTGCCTCCCTATGTTGGTTTGCTGGGATAGCATCTTCAGTTGTAGGTGGTCAAGAGAACGAGGGTAGAGTGGTTATCAGCGAGGAGCTTCTACCAGATGGAATGGACTACTTTGAGGTCATGACCTTGAGTTTAACTGAGACAAAAGTGGAAGAGTGCTGCTGCTGCTGTAGAAGTAGTGGCCACAAAAAGGAAGAGCCGGGTACCACTTCATTACCAAATCAGCCAAGGAAAGGCCGGAAGAGGGGAAGGCAGCGGAAGGATTTCCAGAGTGAAATTCTCCCAAACCTTGCTTCTTTGTCAAGGCATGAGGTGACCGAAGATCTTCAGACAATAGAAAAGTTAATTGAGTCTTCTGGTACAACAAGGTCGGTTAGATGCGCAACTAAACTTGGGTTGGCGAGGGGGAGGAAAAGGTCCAGCATTTCTCCGTCCACTGTAACCGAGAATAGTTTGGAATCACTGTTGACGCAGATTGGTGCCAAAAGTCAGTTCGGAAAAGGGGAGAGAAGTATGATTTGTTGGGGACAGGTAACTAGGCGGCATAGAGGGCAGAGATATCCAGTTAGCAAGCCTTGGCTTATTTCAGGCCAAGCATGGCAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF863

Protein Analysis

963

Amino Acids

106.46

Weight (kDa)

6.01

Isoelectric Point (pI)

56.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF863	PF05904	131 - 258	8.8e-14	Plant protein of unknown function (DUF863)
DUF863	PF05904	277 - 602	1.1e-19	Plant protein of unknown function (DUF863)
DUF863	PF05904	601 - 948	2.3e-59	Plant protein of unknown function (DUF863)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003065)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380 FvH4_5g14380
malus_domestica	MD06G1199000.v1.1 MD14G1206400.v1.1
prunus_persica	Prupe.5G199800_v2.0.a1 Prupe.5G199800_v2.0.a1 Prupe.5G199800_v2.0.a1 Prupe.5G199800_v2.0.a1 Prupe.5G199800_v2.0.a1
pyrus_communis	pycom06g17590 pycom14g17090
rosa_chinensis	RchiOBHm_Chr7g0182271 RchiOBHm_Chr7g0182291
rosa_laevigata	RLG00000005135
rosa_multiflora	Rmu_sc0000003.1_g000008
rosa_roxburghii	Rroxscaffold_3G00271350 Rroxscaffold_3G00271360
rosa_rugosa	Rorug06G0446600 Rorug06G0446600 Rorug06G0446700 Rorug06G0446800
rosa_samantha	Rh7AG049700 Rh7BG049800 Rh7CG051100 Rh7DG049000
rosa_wichuraiana	Rw7G004080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2047
Acc16I	TGCGCA	1 cut(s)	2662
Acc36I	ACCTGC	1 cut(s)	2047
Acc65I	GGTACC	1 cut(s)	2483
AccB1I	GGYRCC	3 cut(s)	2035, 2483, 2759
AccI	GTMKAC	2 cut(s)	1111, 1787
AccIII	TCCGGA	3 cut(s)	667, 1494, 2134
AciI	CCGC	8 cut(s)	572, 1149, 1554, 1619, 1748, 2156, 2536, 2821
AclWI	GGATC	6 cut(s)	564, 997, 1355, 1486, 1660, 2126
AcoI	YGGCCR	2 cut(s)	1792, 2461
AcuI	CTGAAG	6 cut(s)	510, 648, 815, 1845, 2301, 2591
AfaI	GTAC	7 cut(s)	146, 1245, 1305, 1315, 1857, 2485, 2641
AfiI	CCNNNNNNNGG	8 cut(s)	824, 1749, 1750, 2075, 2295, 2470, 2513, 2524
AflII	CTTAAG	1 cut(s)	1571
AjnI	CCWGG	7 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119
AjuI	GAANNNNNNNTTGG	8 cut(s)	536, 568, 1138, 1170, 2557, 2589, 2756, 2788
Alw21I	GWGCWC	3 cut(s)	332, 1041, 1169
Alw26I	GTCTC	5 cut(s)	327, 498, 1572, 2096, 2414
Alw44I	GTGCAC	1 cut(s)	1165
AlwI	GGATC	6 cut(s)	564, 997, 1355, 1486, 1660, 2126
Aor13HI	TCCGGA	3 cut(s)	667, 1494, 2134
AoxI	GGCC	7 cut(s)	826, 1212, 1340, 1792, 2461, 2515, 2869
ApaLI	GTGCAC	1 cut(s)	1165
ApeKI	GCWGC	7 cut(s)	1046, 1226, 2198, 2439, 2442, 2445, 2533
ArsI	GACNNNNNNTTYG	6 cut(s)	301, 333, 478, 510, 1550, 1582
AseI	ATTAAT	1 cut(s)	245
Asp700I	GAANNNNTTC	6 cut(s)	269, 548, 622, 1182, 2543, 2604
Asp718I	GGTACC	1 cut(s)	2483
AspLEI	GCGC	1 cut(s)	2663
AspS9I	GGNCC	7 cut(s)	133, 211, 1142, 1212, 1497, 2253, 2697
AsuC2I	CCSGG	1 cut(s)	2481
AsuHPI	GGTGA	5 cut(s)	423, 698, 1367, 1989, 2605
AvaII	GGWCC	6 cut(s)	133, 211, 1142, 1497, 2253, 2697
AxyI	CCTNAGG	3 cut(s)	76, 636, 1188
BaeGI	GKGCMC	1 cut(s)	1169
BaeI	ACNNNNGTAYC	2 cut(s)	1287, 1320
BalI	TGGCCA	2 cut(s)	1794, 2463
BanI	GGYRCC	3 cut(s)	2035, 2483, 2759
BanII	GRGCYC	2 cut(s)	332, 1041
BbsI	GAAGAC	2 cut(s)	238, 2625
Bbv12I	GWGCWC	3 cut(s)	332, 1041, 1169
BbvI	GCAGC	7 cut(s)	1058, 1238, 2210, 2426, 2429, 2432, 2545
BccI	CCATC	7 cut(s)	286, 608, 707, 1192, 1540, 1982, 2372
BceAI	ACGGC	1 cut(s)	919
BciT130I	CCWGG	7 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121
BcnI	CCSGG	1 cut(s)	2481
BcoDI	GTCTC	5 cut(s)	327, 498, 1572, 2096, 2414
BfaI	CTAG	4 cut(s)	1020, 1389, 2043, 2817
BfmI	CTRYAG	3 cut(s)	1112, 1364, 2449
BfrI	CTTAAG	1 cut(s)	1571
BfuAI	ACCTGC	1 cut(s)	2047
BglII	AGATCT	2 cut(s)	1385, 2602
BisI	GCNGC	9 cut(s)	1047, 1227, 1620, 2199, 2440, 2443, 2446, 2534, 2822
BlsI	GCNGC	9 cut(s)	1048, 1228, 1621, 2200, 2441, 2444, 2447, 2535, 2823
BmcAI	AGTACT	1 cut(s)	146
Bme1390I	CCNGG	8 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121, 2481
Bme18I	GGWCC	6 cut(s)	133, 211, 1142, 1497, 2253, 2697
BmgT120I	GGNCC	7 cut(s)	133, 211, 1142, 1212, 1497, 2253, 2697
BmiI	GGNNCC	6 cut(s)	1213, 1262, 1441, 2037, 2485, 2761
BmrFI	CCNGG	8 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121, 2481
BmrI	ACTGGG	1 cut(s)	1438
BmsI	GCATC	8 cut(s)	13, 565, 679, 764, 1606, 1882, 2321, 2648
BmuI	ACTGGG	1 cut(s)	1438
BpiI	GAAGAC	2 cut(s)	238, 2625
BpmI	CTGGAG	3 cut(s)	969, 1548, 2004
BpuEI	CTTGAG	5 cut(s)	161, 821, 1025, 1750, 2428
BpuMI	CCSGG	1 cut(s)	2481
BsaJI	CCNNGG	7 cut(s)	61, 1343, 1688, 1743, 2256, 2507, 2855
BsaWI	WCCGGW	4 cut(s)	667, 1494, 1649, 2134
Bsc4I	CCNNNNNNNGG	8 cut(s)	824, 1749, 1750, 2075, 2295, 2470, 2513, 2524
Bse1I	ACTGG	9 cut(s)	43, 427, 1444, 1500, 1606, 1795, 1987, 2023, 2843
Bse21I	CCTNAGG	3 cut(s)	76, 636, 1188
Bse3DI	GCAATG	1 cut(s)	1441
BseAI	TCCGGA	3 cut(s)	667, 1494, 2134
BseBI	CCWGG	7 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121
BseDI	CCNNGG	7 cut(s)	61, 1343, 1688, 1743, 2256, 2507, 2855
BseGI	GGATG	6 cut(s)	39, 256, 580, 1621, 1733, 2134
BseLI	CCNNNNNNNGG	8 cut(s)	824, 1749, 1750, 2075, 2295, 2470, 2513, 2524
BseMI	GCAATG	1 cut(s)	1441
BseMII	CTCAG	6 cut(s)	90, 650, 942, 1202, 1643, 2409
BseNI	ACTGG	9 cut(s)	43, 427, 1444, 1500, 1606, 1795, 1987, 2023, 2843
BseRI	GAGGAG	3 cut(s)	1049, 2091, 2378
BseSI	GKGCMC	1 cut(s)	1169
BseXI	GCAGC	7 cut(s)	1058, 1238, 2210, 2426, 2429, 2432, 2545
BseYI	CCCAGC	1 cut(s)	2303
BsgI	GTGCAG	1 cut(s)	1245
BshFI	GGCC	7 cut(s)	828, 1214, 1342, 1794, 2463, 2517, 2871
BshNI	GGYRCC	3 cut(s)	2035, 2483, 2759
BsiHKAI	GWGCWC	3 cut(s)	332, 1041, 1169
BsiSI	CCGG	6 cut(s)	668, 1495, 1650, 2135, 2480, 2518
BslFI	GGGAC	2 cut(s)	470, 2820
BslI	CCNNNNNNNGG	8 cut(s)	824, 1749, 1750, 2075, 2295, 2470, 2513, 2524
BsmAI	GTCTC	5 cut(s)	327, 498, 1572, 2096, 2414
BsmFI	GGGAC	2 cut(s)	470, 2820
BsmI	GAATGC	1 cut(s)	2200
BsnI	GGCC	7 cut(s)	828, 1214, 1342, 1794, 2463, 2517, 2871
Bsp1286I	GDGCHC	3 cut(s)	332, 1041, 1169
Bsp13I	TCCGGA	3 cut(s)	667, 1494, 2134
Bsp1407I	TGTACA	1 cut(s)	1243
Bsp143I	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
BspACI	CCGC	8 cut(s)	572, 1149, 1554, 1619, 1748, 2156, 2536, 2821
BspANI	GGCC	7 cut(s)	828, 1214, 1342, 1794, 2463, 2517, 2871
BspCNI	CTCAG	6 cut(s)	89, 649, 943, 1201, 1642, 2410
BspEI	TCCGGA	3 cut(s)	667, 1494, 2134
BspHI	TCATGA	1 cut(s)	2400
BspLI	GGNNCC	6 cut(s)	1213, 1262, 1441, 2037, 2485, 2761
BspMI	ACCTGC	1 cut(s)	2047
BspPI	GGATC	6 cut(s)	564, 997, 1355, 1486, 1660, 2126
BspQI	GCTCTTC	1 cut(s)	2469
BspT107I	GGYRCC	3 cut(s)	2035, 2483, 2759
BspTI	CTTAAG	1 cut(s)	1571
BsrDI	GCAATG	1 cut(s)	1441
BsrGI	TGTACA	1 cut(s)	1243
BsrI	ACTGG	9 cut(s)	43, 427, 1444, 1500, 1606, 1795, 1987, 2023, 2843
BssECI	CCNNGG	7 cut(s)	61, 1343, 1688, 1743, 2256, 2507, 2855
BssMI	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
BssT1I	CCWWGG	3 cut(s)	1688, 2507, 2855
Bst2UI	CCWGG	7 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121
Bst4CI	ACNGT	6 cut(s)	107, 1260, 1336, 1368, 2719, 2745
Bst6I	CTCTTC	3 cut(s)	2427, 2469, 2516
BstAFI	CTTAAG	1 cut(s)	1571
BstAPI	GCANNNNNTGC	1 cut(s)	172
BstAUI	TGTACA	1 cut(s)	1243
BstC8I	GCNNGC	4 cut(s)	391, 1169, 1685, 2852
BstDEI	CTNAG	8 cut(s)	76, 607, 636, 843, 951, 1188, 1629, 2418
BstDSI	CCRYGG	1 cut(s)	2256
BstEII	GGTNACC	1 cut(s)	2593
BstENI	CCTNNNNNAGG	1 cut(s)	822
BstF5I	GGATG	6 cut(s)	39, 256, 580, 1621, 1733, 2134
BstHHI	GCGC	1 cut(s)	2663
BstKTI	GATC	8 cut(s)	559, 992, 1350, 1388, 1494, 1655, 2134, 2605
BstMAI	GTCTC	5 cut(s)	327, 498, 1572, 2096, 2414
BstMBI	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
BstMWI	GCNNNNNNNGC	6 cut(s)	172, 1036, 1277, 2309, 2445, 2830
BstNI	CCWGG	7 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121
BstNSI	RCATGY	2 cut(s)	393, 471
BstPI	GGTNACC	1 cut(s)	2593
BstSCI	CCNGG	8 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119, 2479
BstSFI	CTRYAG	3 cut(s)	1112, 1364, 2449
BstSLI	GKGCMC	1 cut(s)	1169
BstV1I	GCAGC	7 cut(s)	1058, 1238, 2210, 2426, 2429, 2432, 2545
BstV2I	GAAGAC	2 cut(s)	238, 2625
BstX2I	RGATCY	4 cut(s)	556, 989, 1385, 2602
BstYI	RGATCY	4 cut(s)	556, 989, 1385, 2602
Bsu36I	CCTNAGG	3 cut(s)	76, 636, 1188
BsuRI	GGCC	7 cut(s)	828, 1214, 1342, 1794, 2463, 2517, 2871
BtgI	CCRYGG	1 cut(s)	2256
BtsCI	GGATG	6 cut(s)	39, 256, 580, 1621, 1733, 2134
BtsI	GCAGTG	1 cut(s)	1228
BtsIMutI	CAGTG	9 cut(s)	50, 708, 939, 1228, 1507, 1637, 1890, 2715, 2741
BveI	ACCTGC	1 cut(s)	2047
Cac8I	GCNNGC	4 cut(s)	391, 1169, 1685, 2852
CciI	TCATGA	1 cut(s)	2400
CfoI	GCGC	1 cut(s)	2663
Cfr13I	GGNCC	7 cut(s)	133, 211, 1142, 1212, 1497, 2253, 2697
CseI	GACGC	1 cut(s)	2758
Csp6I	GTAC	7 cut(s)	145, 1244, 1304, 1314, 1856, 2484, 2640
CviQI	GTAC	7 cut(s)	145, 1244, 1304, 1314, 1856, 2484, 2640
DdeI	CTNAG	8 cut(s)	76, 607, 636, 843, 951, 1188, 1629, 2418
DpnI	GATC	8 cut(s)	558, 991, 1349, 1387, 1493, 1654, 2133, 2604
DpnII	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
DraI	TTTAAA	1 cut(s)	1866
EaeI	YGGCCR	2 cut(s)	1792, 2461
Eam1104I	CTCTTC	3 cut(s)	2427, 2469, 2516
EarI	CTCTTC	3 cut(s)	2427, 2469, 2516
EciI	GGCGGA	1 cut(s)	2145
Ecl136II	GAGCTC	2 cut(s)	330, 1039
Eco130I	CCWWGG	3 cut(s)	1688, 2507, 2855
Eco147I	AGGCCT	2 cut(s)	828, 1342
Eco24I	GRGCYC	2 cut(s)	332, 1041
Eco32I	GATATC	1 cut(s)	2840
Eco47I	GGWCC	6 cut(s)	133, 211, 1142, 1497, 2253, 2697
Eco53kI	GAGCTC	2 cut(s)	330, 1039
Eco57I	CTGAAG	6 cut(s)	510, 648, 815, 1845, 2301, 2591
Eco81I	CCTNAGG	3 cut(s)	76, 636, 1188
Eco91I	GGTNACC	1 cut(s)	2593
EcoICRI	GAGCTC	2 cut(s)	330, 1039
EcoNI	CCTNNNNNAGG	1 cut(s)	822
EcoO109I	RGGNCCY	2 cut(s)	211, 1212
EcoO65I	GGTNACC	1 cut(s)	2593
EcoRI	GAATTC	5 cut(s)	269, 973, 1081, 1459, 2022
EcoRII	CCWGG	7 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119
EcoRV	GATATC	1 cut(s)	2840
EcoT14I	CCWWGG	3 cut(s)	1688, 2507, 2855
EcoT22I	ATGCAT	2 cut(s)	6, 580
EcoT38I	GRGCYC	2 cut(s)	332, 1041
ErhI	CCWWGG	3 cut(s)	1688, 2507, 2855
FalI	AAGNNNNNCTT	6 cut(s)	107, 139, 536, 568, 1138, 1170
FaqI	GGGAC	2 cut(s)	470, 2820
FauI	CCCGC	1 cut(s)	1741
FauNDI	CATATG	1 cut(s)	1174
FblI	GTMKAC	2 cut(s)	1111, 1787
Fnu4HI	GCNGC	9 cut(s)	1047, 1227, 1620, 2199, 2440, 2443, 2446, 2534, 2822
FokI	GGATG	6 cut(s)	26, 263, 587, 1628, 1740, 2141
FriOI	GRGCYC	2 cut(s)	332, 1041
Fsp4HI	GCNGC	9 cut(s)	1047, 1227, 1620, 2199, 2440, 2443, 2446, 2534, 2822
FspBI	CTAG	4 cut(s)	1020, 1389, 2043, 2817
FspI	TGCGCA	1 cut(s)	2662
GlaI	GCGC	1 cut(s)	2662
GluI	GCNGC	9 cut(s)	1047, 1227, 1620, 2199, 2440, 2443, 2446, 2534, 2822
GsaI	CCCAGC	1 cut(s)	2307
GsuI	CTGGAG	3 cut(s)	969, 1548, 2004
HaeIII	GGCC	7 cut(s)	828, 1214, 1342, 1794, 2463, 2517, 2871
HapII	CCGG	6 cut(s)	668, 1495, 1650, 2135, 2480, 2518
HgaI	GACGC	1 cut(s)	2758
HhaI	GCGC	1 cut(s)	2663
Hin6I	GCGC	1 cut(s)	2661
HinP1I	GCGC	1 cut(s)	2661
HincII	GTYRAC	3 cut(s)	309, 747, 2748
HindII	GTYRAC	3 cut(s)	309, 747, 2748
HindIII	AAGCTT	3 cut(s)	797, 1668, 1703
HpaII	CCGG	6 cut(s)	668, 1495, 1650, 2135, 2480, 2518
HphI	GGTGA	5 cut(s)	423, 698, 1367, 1989, 2605
HpyCH4III	ACNGT	6 cut(s)	107, 1260, 1336, 1368, 2719, 2745
HpyCH4IV	ACGT	2 cut(s)	360, 743
HpyF10VI	GCNNNNNNNGC	6 cut(s)	172, 1036, 1277, 2309, 2445, 2830
HpyF3I	CTNAG	8 cut(s)	76, 607, 636, 843, 951, 1188, 1629, 2418
HpySE526I	ACGT	2 cut(s)	360, 743
HspAI	GCGC	1 cut(s)	2661
Kpn2I	TCCGGA	3 cut(s)	667, 1494, 2134
KpnI	GGTACC	1 cut(s)	2487
Kzo9I	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
LguI	GCTCTTC	1 cut(s)	2469
LmnI	GCTCC	5 cut(s)	327, 1036, 1151, 1445, 2365
Lsp1109I	GCAGC	7 cut(s)	1058, 1238, 2210, 2426, 2429, 2432, 2545
LweI	GCATC	8 cut(s)	13, 565, 679, 764, 1606, 1882, 2321, 2648
MaeI	CTAG	4 cut(s)	1020, 1389, 2043, 2817
MaeII	ACGT	2 cut(s)	360, 743
MaeIII	GTNAC	7 cut(s)	429, 1073, 1355, 1502, 2593, 2719, 2812
MalI	GATC	8 cut(s)	558, 991, 1349, 1387, 1493, 1654, 2133, 2604
MboI	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
MflI	RGATCY	4 cut(s)	556, 989, 1385, 2602
MhlI	GDGCHC	3 cut(s)	332, 1041, 1169
MlsI	TGGCCA	2 cut(s)	1794, 2463
MluNI	TGGCCA	2 cut(s)	1794, 2463
MlyI	GAGTC	3 cut(s)	500, 1793, 2639
MmeI	TCCRAC	3 cut(s)	498, 1082, 1428
Mox20I	TGGCCA	2 cut(s)	1794, 2463
Mph1103I	ATGCAT	2 cut(s)	6, 580
MroI	TCCGGA	3 cut(s)	667, 1494, 2134
MroXI	GAANNNNTTC	6 cut(s)	269, 548, 622, 1182, 2543, 2604
MscI	TGGCCA	2 cut(s)	1794, 2463
MslI	CAYNNNNRTG	3 cut(s)	436, 1285, 1404
Msp20I	TGGCCA	2 cut(s)	1794, 2463
MspA1I	CMGCKG	3 cut(s)	572, 1049, 2536
MspCI	CTTAAG	1 cut(s)	1571
MspI	CCGG	6 cut(s)	668, 1495, 1650, 2135, 2480, 2518
MspR9I	CCNGG	8 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121, 2481
Mva1269I	GAATGC	1 cut(s)	2200
MvaI	CCWGG	7 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121
MwoI	GCNNNNNNNGC	6 cut(s)	172, 1036, 1277, 2309, 2445, 2830
NciI	CCSGG	1 cut(s)	2481
NdeI	CATATG	1 cut(s)	1174
NdeII	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
NlaIV	GGNNCC	6 cut(s)	1213, 1262, 1441, 2037, 2485, 2761
NmuCI	GTSAC	4 cut(s)	429, 1355, 1502, 2593
NsbI	TGCGCA	1 cut(s)	2662
NsiI	ATGCAT	2 cut(s)	6, 580
NspI	RCATGY	2 cut(s)	393, 471
PaeI	GCATGC	1 cut(s)	393
PagI	TCATGA	1 cut(s)	2400
PaqCI	CACCTGC	1 cut(s)	2047
PceI	AGGCCT	2 cut(s)	828, 1342
PciSI	GCTCTTC	1 cut(s)	2469
PctI	GAATGC	1 cut(s)	2200
PdmI	GAANNNNTTC	6 cut(s)	269, 548, 622, 1182, 2543, 2604
PfoI	TCCNGGA	1 cut(s)	985
PkrI	GCNGC	9 cut(s)	1048, 1228, 1621, 2200, 2441, 2444, 2447, 2535, 2823
PleI	GAGTC	3 cut(s)	500, 1792, 2638
PpsI	GAGTC	3 cut(s)	500, 1792, 2638
PpuMI	RGGWCCY	1 cut(s)	211
PshBI	ATTAAT	1 cut(s)	245
Psp124BI	GAGCTC	2 cut(s)	332, 1041
Psp5II	RGGWCCY	1 cut(s)	211
Psp6I	CCWGG	7 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119
PspEI	GGTNACC	1 cut(s)	2593
PspFI	CCCAGC	1 cut(s)	2303
PspGI	CCWGG	7 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119
PspN4I	GGNNCC	6 cut(s)	1213, 1262, 1441, 2037, 2485, 2761
PspPI	GGNCC	7 cut(s)	133, 211, 1142, 1212, 1497, 2253, 2697
PspPPI	RGGWCCY	1 cut(s)	211
PsrI	GAACNNNNNNTAC	2 cut(s)	802, 834
PsuI	RGATCY	4 cut(s)	556, 989, 1385, 2602
PvuII	CAGCTG	1 cut(s)	1049
RsaI	GTAC	7 cut(s)	146, 1245, 1305, 1315, 1857, 2485, 2641
RsaNI	GTAC	7 cut(s)	145, 1244, 1304, 1314, 1856, 2484, 2640
RseI	CAYNNNNRTG	3 cut(s)	436, 1285, 1404
SacI	GAGCTC	2 cut(s)	332, 1041
SapI	GCTCTTC	1 cut(s)	2469
SatI	GCNGC	9 cut(s)	1047, 1227, 1620, 2199, 2440, 2443, 2446, 2534, 2822
Sau3AI	GATC	8 cut(s)	556, 989, 1347, 1385, 1491, 1652, 2131, 2602
Sau96I	GGNCC	7 cut(s)	133, 211, 1142, 1212, 1497, 2253, 2697
ScaI	AGTACT	1 cut(s)	146
SchI	GAGTC	3 cut(s)	500, 1793, 2639
ScrFI	CCNGG	8 cut(s)	63, 865, 987, 1344, 1527, 1745, 2121, 2481
SduI	GDGCHC	3 cut(s)	332, 1041, 1169
SfaNI	GCATC	8 cut(s)	13, 565, 679, 764, 1606, 1882, 2321, 2648
SfcI	CTRYAG	3 cut(s)	1112, 1364, 2449
SinI	GGWCC	6 cut(s)	133, 211, 1142, 1497, 2253, 2697
SmiMI	CAYNNNNRTG	3 cut(s)	436, 1285, 1404
SmlI	CTYRAG	6 cut(s)	176, 800, 1040, 1571, 1765, 2407
SmoI	CTYRAG	6 cut(s)	176, 800, 1040, 1571, 1765, 2407
SphI	GCATGC	1 cut(s)	393
SseBI	AGGCCT	2 cut(s)	828, 1342
SsiI	CCGC	8 cut(s)	572, 1149, 1554, 1619, 1748, 2156, 2536, 2821
SspI	AATATT	1 cut(s)	192
SspMI	CTAG	4 cut(s)	1020, 1389, 2043, 2817
SstI	GAGCTC	2 cut(s)	332, 1041
StuI	AGGCCT	2 cut(s)	828, 1342
StyD4I	CCNGG	8 cut(s)	61, 863, 985, 1342, 1525, 1743, 2119, 2479
StyI	CCWWGG	3 cut(s)	1688, 2507, 2855
TaaI	ACNGT	6 cut(s)	107, 1260, 1336, 1368, 2719, 2745
TaiI	ACGT	2 cut(s)	363, 746
TaqI	TCGA	4 cut(s)	141, 273, 448, 561
TaqII	GACCGA	2 cut(s)	1097, 2612
TatI	WGTACW	2 cut(s)	144, 1243
TauI	GCSGC	2 cut(s)	1622, 2824
TscAI	CASTG	9 cut(s)	50, 715, 946, 1228, 1507, 1637, 1890, 2722, 2748
TseFI	GTSAC	4 cut(s)	429, 1355, 1502, 2593
TseI	GCWGC	7 cut(s)	1046, 1226, 2198, 2439, 2442, 2445, 2533
Tsp45I	GTSAC	4 cut(s)	429, 1355, 1502, 2593
TspGWI	ACGGA	4 cut(s)	146, 243, 2273, 2700
TspRI	CASTG	9 cut(s)	50, 715, 946, 1228, 1507, 1637, 1890, 2722, 2748
Vha464I	CTTAAG	1 cut(s)	1571
VneI	GTGCAC	1 cut(s)	1165
VpaK11BI	GGWCC	6 cut(s)	133, 211, 1142, 1497, 2253, 2697
VspI	ATTAAT	1 cut(s)	245
XagI	CCTNNNNNAGG	1 cut(s)	822
XceI	RCATGY	2 cut(s)	393, 471
XmiI	GTMKAC	2 cut(s)	1111, 1787
XmnI	GAANNNNTTC	6 cut(s)	269, 548, 622, 1182, 2543, 2604
XspI	CTAG	4 cut(s)	1020, 1389, 2043, 2817
ZrmI	AGTACT	1 cut(s)	146
Zsp2I	ATGCAT	2 cut(s)	6, 580

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.