RchiOBHm_Chr7g0182271

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0003065
Physical Location & Seq
Forward (+)
3589198 .. 3593997
4800 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16260

Sequence Viewer

Length: 900 bp
ATGTATCAGAATTCAGACTTGCTGGAAATGGGAGCTCATATTCCATGCAGCAGCTATGCCCCAGTATATATCCCCACAATGAATCATAACTTAAGTGCTAATGGCAGTGAGAGGAATCATAATTTGACTTCTGACCAGCTTTTTTGGTATTACAAGGAGGTGTTCAAGCAGAAAATGCTCAATCAAGAAATGATATTTAAGAATCAGATTCTGGACCTCCACCGCCTTTATGGAAGACAAAGAGAACTAATGGATGATATAACACGGAATGAATTAGATAAACATAATCTGGGCACCGAGGTTTCAAGGCGGACTACTTCTTTGTCTCAGACTTCCCTTGTCTGTGTTCAAAAGACATTCAACATCCCTGCATGCAGTCAAGTATCTATTTCAGGTGCTGAAAATATCCAGTCACCTTCATGTTTTCTTCAAGGGAGGAACATACGGGCATGCTCTTATCCTGCTCAAACTGAAGGCCATTCAGGAGACCGTGAATTTTTGAAATCCAAATACAAGAAACCTCGGAAGAACTTGGACCTTCGACTTCCAGCTGATACAATATACATTGATAGTGAAGAAGAGGAATTGTTAGAAGACGGAAAGGTTTCTGAAGCAGCTGAGGTATCAAATTATCCTACTAAGAGAATTCCCGAGGTTGTGTGTAATGGTGATGCAACTAATGGATTCAACTCCGTTTCCAATTATGCCTCAAGTGCTTCATTAGAAAAAGGTTGGGTTGACTTAAATTATCTGTATAAGCTACAGGAAAAAACAGCTCCTGCATCCGATAGTTTTGAGGGGCCCACTGCTGTCGAAGCTTACTCAAATATTCTACTGCAAGGCACAGAAAGAAAACAGGAGTGGTTGTCATCTTATAATTACAATGCTGGGCCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

299

Amino Acids

33.87

Weight (kDa)

5.49

Isoelectric Point (pI)

54.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 125 - 278 5.9e-12 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
AccB1I GGYRCC 1 cut(s) 293
AciI CCGC 2 cut(s) 223, 310
AcsI RAATTY 3 cut(s) 10, 494, 645
AcuI CTGAAG 2 cut(s) 492, 630
AflII CTTAAG 1 cut(s) 91
AgsI TTSAA 7 cut(s) 166, 306, 350, 361, 431, 502, 688
AluBI AGCT 8 cut(s) 35, 54, 139, 551, 617, 760, 776, 818
AluI AGCT 8 cut(s) 35, 54, 139, 551, 617, 760, 776, 818
Alw21I GWGCWC 1 cut(s) 37
Alw26I GTCTC 2 cut(s) 330, 480
AlwNI CAGNNNCTG 3 cut(s) 211, 398, 779
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 3 cut(s) 475, 800, 890
ApaI GGGCCC 2 cut(s) 804, 894
ApeKI GCWGC 3 cut(s) 48, 51, 614
ApoI RAATTY 3 cut(s) 10, 494, 645
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
Asp700I GAANNNNTTC 1 cut(s) 604
AspS9I GGNCC 6 cut(s) 214, 535, 800, 801, 890, 891
AsuHPI GGTGA 2 cut(s) 405, 680
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 2 cut(s) 214, 535
BaeGI GKGCMC 3 cut(s) 296, 804, 894
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 3 cut(s) 37, 804, 894
BbsI GAAGAC 2 cut(s) 241, 600
Bbv12I GWGCWC 1 cut(s) 37
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 3 cut(s) 60, 63, 626
BcoDI GTCTC 2 cut(s) 330, 480
BfmI CTRYAG 1 cut(s) 761
BfrI CTTAAG 1 cut(s) 91
BisI GCNGC 3 cut(s) 49, 52, 615
BlsI GCNGC 3 cut(s) 50, 53, 616
Bme18I GGWCC 2 cut(s) 214, 535
BmeT110I CYCGRG 1 cut(s) 650
BmgT120I GGNCC 6 cut(s) 214, 535, 800, 801, 890, 891
BmiI GGNNCC 5 cut(s) 295, 801, 802, 892, 893
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 2 cut(s) 661, 791
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 2 cut(s) 241, 600
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 1 cut(s) 694
BsaI GGTCTC 1 cut(s) 480
BsaJI CCNNGG 3 cut(s) 297, 521, 651
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bse1I ACTGG 2 cut(s) 62, 409
BseDI CCNNGG 3 cut(s) 297, 521, 651
BseGI GGATG 3 cut(s) 259, 363, 782
BseMII CTCAG 2 cut(s) 341, 609
BseNI ACTGG 2 cut(s) 62, 409
BseSI GKGCMC 3 cut(s) 296, 804, 894
BseXI GCAGC 3 cut(s) 60, 63, 626
BseYI CCCAGC 1 cut(s) 887
BshFI GGCC 3 cut(s) 477, 802, 892
BshNI GGYRCC 1 cut(s) 293
BsiHKAI GWGCWC 1 cut(s) 37
BsiHKCI CYCGRG 1 cut(s) 650
BsmAI GTCTC 2 cut(s) 330, 480
BsnI GGCC 3 cut(s) 477, 802, 892
Bso31I GGTCTC 1 cut(s) 480
BsoBI CYCGRG 1 cut(s) 650
Bsp120I GGGCCC 2 cut(s) 800, 890
Bsp1286I GDGCHC 4 cut(s) 37, 296, 804, 894
BspACI CCGC 2 cut(s) 223, 310
BspANI GGCC 3 cut(s) 477, 802, 892
BspCNI CTCAG 2 cut(s) 340, 610
BspLI GGNNCC 5 cut(s) 295, 801, 802, 892, 893
BspT107I GGYRCC 1 cut(s) 293
BspTI CTTAAG 1 cut(s) 91
BspTNI GGTCTC 1 cut(s) 480
BsrI ACTGG 2 cut(s) 62, 409
BssECI CCNNGG 3 cut(s) 297, 521, 651
Bst4CI ACNGT 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 573
BstAFI CTTAAG 1 cut(s) 91
BstAPI GCANNNNNTGC 1 cut(s) 175
BstC8I GCNNGC 2 cut(s) 373, 451
BstDEI CTNAG 3 cut(s) 327, 618, 639
BstF5I GGATG 3 cut(s) 259, 363, 782
BstMAI GTCTC 2 cut(s) 330, 480
BstMWI GCNNNNNNNGC 3 cut(s) 175, 713, 815
BstNSI RCATGY 2 cut(s) 375, 453
BstSFI CTRYAG 1 cut(s) 761
BstSLI GKGCMC 3 cut(s) 296, 804, 894
BstV1I GCAGC 3 cut(s) 60, 63, 626
BstV2I GAAGAC 2 cut(s) 241, 600
BsuRI GGCC 3 cut(s) 477, 802, 892
BtsCI GGATG 3 cut(s) 259, 363, 782
BtsI GCAGTG 2 cut(s) 112, 804
BtsIMutI CAGTG 2 cut(s) 112, 804
Cac8I GCNNGC 2 cut(s) 373, 451
CaiI CAGNNNCTG 3 cut(s) 211, 398, 779
Cfr13I GGNCC 6 cut(s) 214, 535, 800, 801, 890, 891
CviAII CATG 4 cut(s) 45, 372, 420, 450
DdeI CTNAG 3 cut(s) 327, 618, 639
Eam1104I CTCTTC 1 cut(s) 573
EarI CTCTTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 325
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 3 cut(s) 37, 804, 894
Eco31I GGTCTC 1 cut(s) 480
Eco47I GGWCC 2 cut(s) 214, 535
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 2 cut(s) 492, 630
Eco88I CYCGRG 1 cut(s) 650
EcoICRI GAGCTC 1 cut(s) 35
EcoO109I RGGNCCY 2 cut(s) 800, 891
EcoRI GAATTC 2 cut(s) 10, 645
EcoT38I GRGCYC 3 cut(s) 37, 804, 894
FaeI CATG 4 cut(s) 48, 375, 423, 453
FatI CATG 4 cut(s) 44, 371, 419, 449
Fnu4HI GCNGC 3 cut(s) 49, 52, 615
FokI GGATG 3 cut(s) 266, 350, 769
FriOI GRGCYC 3 cut(s) 37, 804, 894
Fsp4HI GCNGC 3 cut(s) 49, 52, 615
GluI GCNGC 3 cut(s) 49, 52, 615
GsaI CCCAGC 1 cut(s) 891
HaeIII GGCC 3 cut(s) 477, 802, 892
Hin1II CATG 4 cut(s) 48, 375, 423, 453
HincII GTYRAC 1 cut(s) 739
HindII GTYRAC 1 cut(s) 739
HindIII AAGCTT 1 cut(s) 816
HinfI GANTC 5 cut(s) 82, 115, 202, 208, 684
HphI GGTGA 2 cut(s) 405, 680
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 8 cut(s) 9, 16, 133, 207, 330, 525, 610, 787
Hpy188III TCNNGA 4 cut(s) 185, 212, 483, 650
Hpy8I GTNNAC 1 cut(s) 739
HpyAV CCTTC 3 cut(s) 426, 467, 548
HpyCH4III ACNGT 1 cut(s) 491
HpyCH4V TGCA 6 cut(s) 48, 371, 375, 674, 782, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 175, 713, 815
HpyF3I CTNAG 3 cut(s) 327, 618, 639
Hsp92II CATG 4 cut(s) 48, 375, 423, 453
LmnI GCTCC 2 cut(s) 32, 781
Lsp1109I GCAGC 3 cut(s) 60, 63, 626
LweI GCATC 2 cut(s) 661, 791
MaeIII GTNAC 1 cut(s) 411
MboII GAAGA 6 cut(s) 246, 419, 538, 587, 590, 605
MhlI GDGCHC 4 cut(s) 37, 296, 804, 894
MroXI GAANNNNTTC 1 cut(s) 604
MseI TTAA 3 cut(s) 92, 198, 743
MslI CAYNNNNRTG 1 cut(s) 418
MspA1I CMGCKG 2 cut(s) 551, 617
MspCI CTTAAG 1 cut(s) 91
MwoI GCNNNNNNNGC 3 cut(s) 175, 713, 815
NlaIII CATG 4 cut(s) 48, 375, 423, 453
NlaIV GGNNCC 5 cut(s) 295, 801, 802, 892, 893
NmuCI GTSAC 1 cut(s) 411
NspI RCATGY 2 cut(s) 375, 453
PaeI GCATGC 2 cut(s) 375, 453
PdmI GAANNNNTTC 1 cut(s) 604
PfeI GAWTC 5 cut(s) 82, 115, 202, 208, 684
PkrI GCNGC 3 cut(s) 50, 53, 616
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 37
PspFI CCCAGC 1 cut(s) 887
PspN4I GGNNCC 5 cut(s) 295, 801, 802, 892, 893
PspOMI GGGCCC 2 cut(s) 800, 890
PspPI GGNCC 6 cut(s) 214, 535, 800, 801, 890, 891
PstNI CAGNNNCTG 3 cut(s) 211, 398, 779
PvuII CAGCTG 2 cut(s) 551, 617
RseI CAYNNNNRTG 1 cut(s) 418
SacI GAGCTC 1 cut(s) 37
SaqAI TTAA 3 cut(s) 92, 198, 743
SatI GCNGC 3 cut(s) 49, 52, 615
Sau96I GGNCC 6 cut(s) 214, 535, 800, 801, 890, 891
SduI GDGCHC 4 cut(s) 37, 296, 804, 894
SfaNI GCATC 2 cut(s) 661, 791
SfcI CTRYAG 1 cut(s) 761
SinI GGWCC 2 cut(s) 214, 535
SmiMI CAYNNNNRTG 1 cut(s) 418
SmlI CTYRAG 2 cut(s) 91, 709
SmoI CTYRAG 2 cut(s) 91, 709
SphI GCATGC 2 cut(s) 375, 453
SsiI CCGC 2 cut(s) 223, 310
SspI AATATT 1 cut(s) 829
SstI GAGCTC 1 cut(s) 37
TaaI ACNGT 1 cut(s) 491
TaqI TCGA 2 cut(s) 541, 813
TfiI GAWTC 5 cut(s) 82, 115, 202, 208, 684
Tru1I TTAA 3 cut(s) 92, 198, 743
Tru9I TTAA 3 cut(s) 92, 198, 743
TscAI CASTG 2 cut(s) 112, 811
TseFI GTSAC 1 cut(s) 411
TseI GCWGC 3 cut(s) 48, 51, 614
Tsp45I GTSAC 1 cut(s) 411
TspDTI ATGAA 4 cut(s) 95, 285, 408, 708
TspGWI ACGGA 3 cut(s) 280, 612, 682
TspRI CASTG 2 cut(s) 112, 811
Vha464I CTTAAG 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 214, 535
XapI RAATTY 3 cut(s) 10, 494, 645
XceI RCATGY 2 cut(s) 375, 453
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmnI GAANNNNTTC 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.