pycom14g17090

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0003065
Physical Location & Seq
Forward (+)
19162715 .. 19164058
1344 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g17090.1

Sequence Viewer

Length: 1344 bp
ATGGATTCCGCAAATGTCAAGTCTCGTAAGGATATTAACTTGAATTGTGTGCCTACTGGCTGTTCTCTGGATGCGGCAGTTTCCCAGTGTTTCCAAGCCACAACTGGATCAGAAAAACTTGAAGCTTCTAGTGAGAGCTTGCCTTGGCATAGGCGAAAGCATAATGGCAAAACTGACAAAGGATGTGAAGATTCAACCCAGGTGGAGGCAAGTGACTTCTCAAGTAAAATAATTCGTGAGTCTACTGGCCACCCCTCCAATTCCATTCTGAACCCACCTGAAGAAGTCAAAAAAAGAAGAAAAGCGGTAGTATTTGATTTAAATGCTGATTGTGATTCAGTGCTTGACGCAGAAATAGAACTTACAGGTCAGGTAGTAGAGAATGAATTTGATAGGAAAGATTTTGGATTTCAAATTGCCCTGAACTCGTCCACAACCGGAGATAAGTTTTCACCAGCACACTCTCTCTCAACTGGAATTCTTTTGGAGGCACCTGCTAGTCCAGAAAACAAGGAGTGTTCTCCACCAAGAGGAGAATCGGACGTAAATCAAGTTGAGACACCTTTTCTATTGCAAGGACAGGATGATCCGGAAAACAATGAGTTTTCTCCGCCGATAATACAATTTGATGAAAACCAAGTTGTCAGGACTGCAGCAGAATCTTTGGTTTCCATTTCATCATCTGAGCTTCGTAGTTGTATCATAAGGACCACAGACAAGCCACTTAAAACTTCATGTGCCTCCCTATGTTGGTTTGCTGGGATAGCATCTTCAGTTGTAGGTGGTCAAGAGAATGAGGGTAGAGTGGTTATCAGCGAGGAGCTTCTACCAGATGGAATGGACTACTTTGAGGTCATGACCTTGAGTTTAACTGAGACAAAAGTGGAAGAGTGCTGCTGCTGCTGCTGTAGAAGTAATGGCCACAAAAAGGAAGAGACGGGTACCACTTCATTACCAAATCAGCCAAGGAAAGGCCGGAAGAGGGGAAGGCAGCGGAAGGATTTCCAGAGTGAAATTCTCCCAAACCTTGCTTCTTTGTCAAGGCATGAGGTGACTGAAGATCTTCAGACAATAGAAAAGTTAATTGAGTCTTCTGGTACAACAAGGTTGGTTAGATGCGCAACTAAACCTGGGTTGGCGAGGGGAAGGAAAAGGTCCAGCATTTCTCCGTCCACTGTAACCGAGAATAGTTTGGAATCACTGATTGGTGCCAAAAGTCAGTTCGGAAAAGAGGAGAGAAGTCTGATTTGTTGGGGAGAGGTAACTAGGCGGCGTAGAGGGCAGAGATATCCAGTTAGCAAGCCTTGGCTCATTTCAGGCCAAGCATGGCAAGAGCGACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

448

Amino Acids

49.35

Weight (kDa)

5.77

Isoelectric Point (pI)

60.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 85 - 431 6.7e-62 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 502
Acc16I TGCGCA 1 cut(s) 1120
Acc36I ACCTGC 1 cut(s) 502
Acc65I GGTACC 1 cut(s) 941
AccB1I GGYRCC 3 cut(s) 490, 941, 1208
AccI GTMKAC 1 cut(s) 242
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 6 cut(s) 9, 74, 305, 611, 994, 1270
AclWI GGATC 2 cut(s) 115, 581
AcoI YGGCCR 2 cut(s) 247, 919
AcsI RAATTY 3 cut(s) 386, 477, 1014
AcuI CTGAAG 4 cut(s) 300, 756, 1049, 1077
AfaI GTAC 2 cut(s) 943, 1099
AfiI CCNNNNNNNGG 6 cut(s) 205, 530, 750, 928, 971, 982
AgsI TTSAA 4 cut(s) 43, 122, 195, 413
AjnI CCWGG 2 cut(s) 198, 1129
AjuI GAANNNNNNNTTGG 6 cut(s) 1015, 1047, 1188, 1205, 1220, 1237
AluBI AGCT 4 cut(s) 125, 138, 688, 823
AluI AGCT 4 cut(s) 125, 138, 688, 823
Alw26I GTCTC 4 cut(s) 27, 551, 869, 929
AlwI GGATC 2 cut(s) 115, 581
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 4 cut(s) 247, 919, 973, 1318
ApeKI GCWGC 6 cut(s) 653, 894, 897, 900, 903, 991
ApoI RAATTY 3 cut(s) 386, 477, 1014
ArsI GACNNNNNNTTYG 1 cut(s) 37
Asp700I GAANNNNTTC 2 cut(s) 1001, 1062
Asp718I GGTACC 1 cut(s) 941
AspLEI GCGC 1 cut(s) 1121
AspS9I GGNCC 2 cut(s) 708, 1155
AsuHPI GGTGA 2 cut(s) 444, 1063
AvaII GGWCC 2 cut(s) 708, 1155
BalI TGGCCA 2 cut(s) 249, 921
BanI GGYRCC 3 cut(s) 490, 941, 1208
BbsI GAAGAC 1 cut(s) 1083
BbvI GCAGC 6 cut(s) 665, 881, 884, 887, 890, 1003
BccI CCATC 1 cut(s) 827
BciT130I CCWGG 2 cut(s) 200, 1131
BcoDI GTCTC 4 cut(s) 27, 551, 869, 929
BfaI CTAG 3 cut(s) 129, 498, 1266
BfmI CTRYAG 2 cut(s) 651, 907
BfuAI ACCTGC 1 cut(s) 502
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 8 cut(s) 75, 654, 895, 898, 901, 904, 992, 1271
BlsI GCNGC 8 cut(s) 76, 655, 896, 899, 902, 905, 993, 1272
Bme1390I CCNGG 2 cut(s) 200, 1131
Bme18I GGWCC 2 cut(s) 708, 1155
BmgT120I GGNCC 2 cut(s) 708, 1155
BmiI GGNNCC 3 cut(s) 492, 943, 1210
BmrFI CCNGG 2 cut(s) 200, 1131
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 3 cut(s) 61, 776, 1106
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 1 cut(s) 1083
BpuEI CTTGAG 2 cut(s) 205, 883
BsaJI CCNNGG 5 cut(s) 143, 198, 965, 1130, 1304
BsaWI WCCGGW 2 cut(s) 437, 589
Bsc4I CCNNNNNNNGG 6 cut(s) 205, 530, 750, 928, 971, 982
Bse1I ACTGG 6 cut(s) 61, 85, 109, 250, 478, 1292
BseAI TCCGGA 1 cut(s) 589
BseBI CCWGG 2 cut(s) 200, 1131
BseDI CCNNGG 5 cut(s) 143, 198, 965, 1130, 1304
BseGI GGATG 3 cut(s) 76, 188, 589
BseLI CCNNNNNNNGG 6 cut(s) 205, 530, 750, 928, 971, 982
BseMII CTCAG 2 cut(s) 675, 864
BseNI ACTGG 6 cut(s) 61, 85, 109, 250, 478, 1292
BseRI GAGGAG 3 cut(s) 546, 833, 1247
BseXI GCAGC 6 cut(s) 665, 881, 884, 887, 890, 1003
BseYI CCCAGC 1 cut(s) 758
BshFI GGCC 4 cut(s) 249, 921, 975, 1320
BshNI GGYRCC 3 cut(s) 490, 941, 1208
BsiSI CCGG 3 cut(s) 438, 590, 976
BslI CCNNNNNNNGG 6 cut(s) 205, 530, 750, 928, 971, 982
BsmAI GTCTC 4 cut(s) 27, 551, 869, 929
BsmBI CGTCTC 1 cut(s) 929
BsnI GGCC 4 cut(s) 249, 921, 975, 1320
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 3 cut(s) 107, 586, 1060
BspACI CCGC 6 cut(s) 9, 74, 305, 611, 994, 1270
BspANI GGCC 4 cut(s) 249, 921, 975, 1320
BspCNI CTCAG 2 cut(s) 676, 865
BspEI TCCGGA 1 cut(s) 589
BspHI TCATGA 1 cut(s) 855
BspLI GGNNCC 3 cut(s) 492, 943, 1210
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 2 cut(s) 115, 581
BspT107I GGYRCC 3 cut(s) 490, 941, 1208
BsrI ACTGG 6 cut(s) 61, 85, 109, 250, 478, 1292
BssECI CCNNGG 5 cut(s) 143, 198, 965, 1130, 1304
BssMI GATC 3 cut(s) 107, 586, 1060
BssT1I CCWWGG 3 cut(s) 143, 965, 1304
Bst2UI CCWGG 2 cut(s) 200, 1131
Bst4CI ACNGT 1 cut(s) 1177
Bst6I CTCTTC 3 cut(s) 882, 927, 974
BstC8I GCNNGC 2 cut(s) 140, 1301
BstDEI CTNAG 2 cut(s) 684, 873
BstF5I GGATG 3 cut(s) 76, 188, 589
BstHHI GCGC 1 cut(s) 1121
BstKTI GATC 3 cut(s) 110, 589, 1063
BstMAI GTCTC 4 cut(s) 27, 551, 869, 929
BstMBI GATC 3 cut(s) 107, 586, 1060
BstMWI GCNNNNNNNGC 4 cut(s) 764, 900, 903, 1279
BstNI CCWGG 2 cut(s) 200, 1131
BstSCI CCNGG 2 cut(s) 198, 1129
BstSFI CTRYAG 2 cut(s) 651, 907
BstV1I GCAGC 6 cut(s) 665, 881, 884, 887, 890, 1003
BstV2I GAAGAC 1 cut(s) 1083
BstX2I RGATCY 1 cut(s) 1060
BstYI RGATCY 1 cut(s) 1060
BsuRI GGCC 4 cut(s) 249, 921, 975, 1320
BtsCI GGATG 3 cut(s) 76, 188, 589
BtsIMutI CAGTG 4 cut(s) 92, 345, 1173, 1199
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 2 cut(s) 140, 1301
CciI TCATGA 1 cut(s) 855
CfoI GCGC 1 cut(s) 1121
Cfr13I GGNCC 2 cut(s) 708, 1155
CseI GACGC 1 cut(s) 356
Csp6I GTAC 2 cut(s) 942, 1098
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 4 cut(s) 735, 856, 1046, 1326
CviQI GTAC 2 cut(s) 942, 1098
DdeI CTNAG 2 cut(s) 684, 873
DpnI GATC 3 cut(s) 109, 588, 1062
DpnII GATC 3 cut(s) 107, 586, 1060
DraI TTTAAA 1 cut(s) 321
EaeI YGGCCR 2 cut(s) 247, 919
Eam1104I CTCTTC 3 cut(s) 882, 927, 974
EarI CTCTTC 3 cut(s) 882, 927, 974
EciI GGCGGA 1 cut(s) 600
Eco130I CCWWGG 3 cut(s) 143, 965, 1304
Eco32I GATATC 1 cut(s) 1289
Eco47I GGWCC 2 cut(s) 708, 1155
Eco57I CTGAAG 4 cut(s) 300, 756, 1049, 1077
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 198, 1129
EcoRV GATATC 1 cut(s) 1289
EcoT14I CCWWGG 3 cut(s) 143, 965, 1304
ErhI CCWWGG 3 cut(s) 143, 965, 1304
Esp3I CGTCTC 1 cut(s) 929
FaeI CATG 4 cut(s) 738, 859, 1049, 1329
FaiI YATR 8 cut(s) 150, 162, 704, 736, 748, 857, 1047, 1327
FatI CATG 4 cut(s) 734, 855, 1045, 1325
FblI GTMKAC 1 cut(s) 242
Fnu4HI GCNGC 8 cut(s) 75, 654, 895, 898, 901, 904, 992, 1271
FokI GGATG 3 cut(s) 83, 195, 596
Fsp4HI GCNGC 8 cut(s) 75, 654, 895, 898, 901, 904, 992, 1271
FspBI CTAG 3 cut(s) 129, 498, 1266
FspI TGCGCA 1 cut(s) 1120
GlaI GCGC 1 cut(s) 1120
GluI GCNGC 8 cut(s) 75, 654, 895, 898, 901, 904, 992, 1271
GsaI CCCAGC 1 cut(s) 762
HaeIII GGCC 4 cut(s) 249, 921, 975, 1320
HapII CCGG 3 cut(s) 438, 590, 976
HgaI GACGC 1 cut(s) 356
HhaI GCGC 1 cut(s) 1121
Hin1II CATG 4 cut(s) 738, 859, 1049, 1329
Hin6I GCGC 1 cut(s) 1119
HinP1I GCGC 1 cut(s) 1119
HindIII AAGCTT 1 cut(s) 123
HinfI GANTC 8 cut(s) 5, 191, 239, 335, 536, 659, 1088, 1196
HpaII CCGG 3 cut(s) 438, 590, 976
HphI GGTGA 2 cut(s) 444, 1063
Hpy166II GTNNAC 3 cut(s) 243, 432, 1173
Hpy188I TCNGA 7 cut(s) 112, 270, 541, 685, 1068, 1226, 1245
Hpy188III TCNNGA 8 cut(s) 68, 236, 503, 590, 646, 788, 856, 1006
Hpy8I GTNNAC 3 cut(s) 243, 432, 1173
Hpy99I CGWCG 1 cut(s) 1341
HpyAV CCTTC 3 cut(s) 981, 991, 1140
HpyCH4III ACNGT 1 cut(s) 1177
HpyCH4IV ACGT 1 cut(s) 543
HpyCH4V TGCA 2 cut(s) 574, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 764, 900, 903, 1279
HpyF3I CTNAG 2 cut(s) 684, 873
HpySE526I ACGT 1 cut(s) 543
Hsp92II CATG 4 cut(s) 738, 859, 1049, 1329
HspAI GCGC 1 cut(s) 1119
Kpn2I TCCGGA 1 cut(s) 589
KpnI GGTACC 1 cut(s) 945
Kzo9I GATC 3 cut(s) 107, 586, 1060
LmnI GCTCC 1 cut(s) 820
Lsp1109I GCAGC 6 cut(s) 665, 881, 884, 887, 890, 1003
LweI GCATC 3 cut(s) 61, 776, 1106
MaeI CTAG 3 cut(s) 129, 498, 1266
MaeII ACGT 1 cut(s) 543
MaeIII GTNAC 4 cut(s) 212, 1051, 1177, 1261
MalI GATC 3 cut(s) 109, 588, 1062
MboI GATC 3 cut(s) 107, 586, 1060
MflI RGATCY 1 cut(s) 1060
MlsI TGGCCA 2 cut(s) 249, 921
MluCI AATT 9 cut(s) 43, 231, 259, 386, 414, 477, 623, 1014, 1083
MluNI TGGCCA 2 cut(s) 249, 921
MlyI GAGTC 2 cut(s) 248, 1097
Mox20I TGGCCA 2 cut(s) 249, 921
MroI TCCGGA 1 cut(s) 589
MroXI GAANNNNTTC 2 cut(s) 1001, 1062
MscI TGGCCA 2 cut(s) 249, 921
MseI TTAA 5 cut(s) 36, 320, 726, 869, 1082
Msp20I TGGCCA 2 cut(s) 249, 921
MspA1I CMGCKG 1 cut(s) 994
MspI CCGG 3 cut(s) 438, 590, 976
MspR9I CCNGG 2 cut(s) 200, 1131
MvaI CCWGG 2 cut(s) 200, 1131
MwoI GCNNNNNNNGC 4 cut(s) 764, 900, 903, 1279
NdeII GATC 3 cut(s) 107, 586, 1060
NlaIII CATG 4 cut(s) 738, 859, 1049, 1329
NlaIV GGNNCC 3 cut(s) 492, 943, 1210
NmuCI GTSAC 2 cut(s) 212, 1051
NsbI TGCGCA 1 cut(s) 1120
PagI TCATGA 1 cut(s) 855
PaqCI CACCTGC 1 cut(s) 502
PdmI GAANNNNTTC 2 cut(s) 1001, 1062
PfeI GAWTC 6 cut(s) 5, 191, 335, 536, 659, 1196
PkrI GCNGC 8 cut(s) 76, 655, 896, 899, 902, 905, 993, 1272
PleI GAGTC 2 cut(s) 247, 1096
PpsI GAGTC 2 cut(s) 247, 1096
Psp6I CCWGG 2 cut(s) 198, 1129
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 2 cut(s) 198, 1129
PspN4I GGNNCC 3 cut(s) 492, 943, 1210
PspPI GGNCC 2 cut(s) 708, 1155
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 1060
RsaI GTAC 2 cut(s) 943, 1099
RsaNI GTAC 2 cut(s) 942, 1098
SaqAI TTAA 5 cut(s) 36, 320, 726, 869, 1082
SatI GCNGC 8 cut(s) 75, 654, 895, 898, 901, 904, 992, 1271
Sau3AI GATC 3 cut(s) 107, 586, 1060
Sau96I GGNCC 2 cut(s) 708, 1155
SchI GAGTC 2 cut(s) 248, 1097
ScrFI CCNGG 2 cut(s) 200, 1131
SfaNI GCATC 3 cut(s) 61, 776, 1106
SfcI CTRYAG 2 cut(s) 651, 907
SinI GGWCC 2 cut(s) 708, 1155
SmiI ATTTAAAT 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 220, 862
SmoI CTYRAG 2 cut(s) 220, 862
Sse9I AATT 9 cut(s) 43, 231, 259, 386, 414, 477, 623, 1014, 1083
SsiI CCGC 6 cut(s) 9, 74, 305, 611, 994, 1270
SspMI CTAG 3 cut(s) 129, 498, 1266
StyD4I CCNGG 2 cut(s) 198, 1129
StyI CCWWGG 3 cut(s) 143, 965, 1304
SwaI ATTTAAAT 1 cut(s) 321
TaaI ACNGT 1 cut(s) 1177
TaiI ACGT 1 cut(s) 546
TasI AATT 9 cut(s) 43, 231, 259, 386, 414, 477, 623, 1014, 1083
TauI GCSGC 2 cut(s) 77, 1273
TfiI GAWTC 6 cut(s) 5, 191, 335, 536, 659, 1196
Tru1I TTAA 5 cut(s) 36, 320, 726, 869, 1082
Tru9I TTAA 5 cut(s) 36, 320, 726, 869, 1082
TscAI CASTG 4 cut(s) 92, 345, 1180, 1206
TseFI GTSAC 2 cut(s) 212, 1051
TseI GCWGC 6 cut(s) 653, 894, 897, 900, 903, 991
Tsp45I GTSAC 2 cut(s) 212, 1051
TspDTI ATGAA 5 cut(s) 399, 645, 666, 723, 939
TspGWI ACGGA 1 cut(s) 1158
TspRI CASTG 4 cut(s) 92, 345, 1180, 1206
VpaK11BI GGWCC 2 cut(s) 708, 1155
XapI RAATTY 3 cut(s) 386, 477, 1014
XcmI CCANNNNNNNNNTGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 242
XmnI GAANNNNTTC 2 cut(s) 1001, 1062
XspI CTAG 3 cut(s) 129, 498, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.