Rh7DG049000

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003065
Physical Location & Seq
Forward (+)
3765847 .. 3776606
10760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG049000.1

Sequence Viewer

Length: 3372 bp
ATGTATCAGAATTCAGACTTGCTGGAAATGGGAGCTCATATTCCATGCAGCAGCTATGCCCCAGTATATATCCCCACAATGAATCATAACTTAAGTGCTAATGGCAGTGAGAGGAATCATAATTTGACTTCTGACCAGCTTTTTTGGTATTACAAGGAGGTGTTCAAGCAGAAAATGCTCAATCAAGAAATGATATTTAAGAATCAGATTCTGGACCTCCACCGCCTTTATGGAAGACAAAGAGAACTAATGGATGATATAACACGGAATGAATTAGATAAACATAATCTGGGCACCGAGGTTTCAAGGCGGACTACTTCTTTGTCTCAGACTTCCCTTGTCTGTGTTCAAAAGACATTCAACATCCCTGCATGCAGTCAAGTATCTATTTCAGGTGCTGAAAATATCCAGTCACCTTCATGTTTTCTTCAAGGGAGGAACATACGGGCATGCTCTTATCCTGCTCAAACTGAAGGCCATTCAGGAGACCGTGAATTTTTGAAATCCAAATACAAGAAACCTCGGAAGAACTTGGACCTTCGACTTCCAGCTGATACAATATACATTGATAGTGAAGAAGAGGAATTGTTAGAAGACGGAAAGGTTTCTGAAGCAGCTGAGGTATCAAATTATCCTACTAAGAGAATTCCCGAGGTTGTGTGTAATGGTGATGCAACTAATGGATTCAACTCCGTTTCCAATTATGCCTCAAGTGCTTCATTAGAAAAAGGTTGGGTTGACTTAAATTATCTGTATAAGCTACAGGAAAAAACAGCTCCTGCATCCGATAGTTTTGAGGGGCCCACTGCTGTCGAAGCTTACTCAAATATTCTACTGCAAGGCACAGAAAGAAAACAGGAGTGGTTGTCATCTTATAATTACAATGCTGGATTTCATGCAGATGACATATCCTCGTCATCTAACTCATTACATAAGAAGCTTACGCAACAGGAACCGTCATCCTTTCTTCACAATCACCAAAATTCGTCGAGTGAAAGAGCAATGATTGCTGTTCAAAACTTCAGAAGAAATTCTGAAACTAAAAATGATCATCAATCTGGACCAAGTGGAGCTTTGATGTGCACATCATCCCAACATATTCCTCATGACTGCATGGAAAATTCTGGGTCCTCAGGCATTGCTGCTTTAAGAAAACCTATGCATGACTTGGCACTTACCCAGATTGCAGTTCAAGCACTCCCATGTCCTAACACCTCTATACCACTCAGTAATAACTGTAAATCATCAACATTAAGGCATGGGCTTGAGGAAGAAAGGTTACAGTTGAAGAAGCATCTGAGGTCTAGTACAAAACATGATGGTCCGGTCTCTGACAGTAGCTTTAGCAATGTCTTTGATTTAGAATCCAACAAATCAGGGGCCCAGCCACCATCCATCAGTTCTGATAATCTGATAAGGGTTAATAACGGTTTAGTGCCTGAACTTCATGGAATTACCAGATACCTGCAGGATTCTGCAAATGTCAATGCTCGGAAGGATATAGACTTGAATTTCATGCCTCCTAGCTGTTCTTCGGATGCAGCAGTTTCCCAGAGTTTACAAACAGCTGGAACAGAAAAGCTTGAAGATTCTAATGAGAGGTTTCCTTTGCATAGACCGAAACTTGATTATTATGGGAATACTGATAAAGGATGTGAAGATTCAAATCAGGCAGAGGCAAGTGACTTCTTGAGTCAACAGATTCATGGGTCTCCTACTTGTGAGACTCCTAACATTTCTGGTGATCACTGTTTTCATGTCTCAGGCTCCAATCACCATCAGAACTCAGTTCAAGATATCAAGGACAGCAAAAGAGCTGTGCTACTTGATTTAAATGTGCCTTGTGATTCTGTGCACAACAACAAAGTAGAACTAACTGTTGATGAACATGTGGAAAAGAGTAAATTTAAGAAAGATGCAGGTTTTGGAGGTCACATTGACCTGAACTCATCCATAAATGATGTAGGTTTTGGAGTTCACATTGACCTGAACACATCCGTAAATGAAGATGAGTTTTCAGCAATGTCATCCTCTTCAACAGAGAGTCTTTTGGAGGCACCTTCTAGTCCAGAAAACAAAGAGTGTTCTCCACCGAGAGGCGTCTCATCTGATGGAAATCAAGTTGACACACCTGATCTAATGTCAGTACTGGAAGCTCCAGAAAACAAGGAGTCTTCTCCACCTACAGGAGAATCTGATGAAAATCATGTTGAGATACCTTTTCCATTGTCAGTACAGGAAGATCTCGAACATAAGGTGCGTTCTCAGCCAACAGAATCTGATGAACAAATTGAGACACCTTTTGCATTGTCAGGGCAGGGAGGTCTGCAGAACAAAGAGTGTTCTCAGCCAATAGAATCTGATGAAAATCAAAGCGTGACACCTTTGCCATTGTCAAATATGGAAAGCAAAGATTGTTCTAAGCCTATGGAATCTGATGAAAATCAAATTGATACACCTTTTCCATCAGGACTGGATGATCTGGAGGGCAAAGATTGTTCTCAGCCTATGGACTCTGATGAAACTCAAATTCAGACACCTTTGCCATTGTCGGGACAGGAAAATCTGGAAACCAAAGAGCGTTCACAGCCAATGGATTCTACTGAAAAACCAATTGAGACACCTTTTCTATTGTCAGGACAGGAAGTGCATCTGGAAGAGGAACTGTTCAGGACTGCTGCAGAATGTTTGGTCACCATTTCATCATCCGTGTGTCATACTTGTTCTGAAAGGACCACATACAAGCCAACTGAAACTTCATCTGATTCCCTAAAGTGGTTTGCTGGTTTAGCGTCTTCAGTTTTAGGCAGTCTAGAAAATGAGGTGGGATGCGGTGATCATCAGGAGGTTCTACCTGATGGAATGGACTACTTTGAGGCCATGACATTGAAGCTACCAGAGTCAAAGGTGGAAGAGTGCTGCTGCTATAGAAGTAATGGCTACAAAGAGGAAGTGATGGTCACAGCTTCATCACAAAGTCAGCCAAAAAAGGGTCGACCTAGAAAAGGAAGGCAACGGAGGAAGGACTTCCAAAGGGAAATTCTCCCTGGCCTTGCTTCTTTGTCAAGGTGTGAGGTGACTGAGGATCTTCAGATCATAGGAACACTAATTGAGGCTTCTGCTGATTGCTCTGAAACAAGATCAGTAAGACATGCAGCTAAATTTGGGTTGGCAAGGGGGAGGAGAAGGTGCACCATTTCTGCTTCCGCTGTAAAAGAGAATACTGCCGCTTCACCACTGAATCACATCGGTGTCAAAAGGCAATTGGAATGTGAGAAGAGAAGCCTAATTGATTGGGGAGAGGTAACTAGGCGGCCTAGAGGGCAGAGAAATTCGGGTAGTGATCTGCGGCTGATTAGAAACAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

1123

Amino Acids

124.32

Weight (kDa)

5.16

Isoelectric Point (pI)

61.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 125 - 312 1.7e-11 Plant protein of unknown function (DUF863)
DUF863 PF05904 295 - 555 8.9e-06 Plant protein of unknown function (DUF863)
DUF863 PF05904 557 - 694 8.2e-08 Plant protein of unknown function (DUF863)
DUF863 PF05904 850 - 1110 9.7e-60 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 876
Acc36I ACCTGC 2 cut(s) 1473, 1910
AccB1I GGYRCC 2 cut(s) 293, 2056
AccI GTMKAC 1 cut(s) 2995
AciI CCGC 7 cut(s) 223, 310, 2832, 3207, 3228, 3313, 3349
AclWI GGATC 1 cut(s) 3093
AcuI CTGAAG 5 cut(s) 492, 630, 1006, 2781, 3074
AcyI GRCGYC 1 cut(s) 2100
AfaI GTAC 3 cut(s) 1309, 2148, 2235
AfiI CCNNNNNNNGG 6 cut(s) 2096, 2186, 2552, 2775, 2990, 3005
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 1888
AjnI CCWGG 1 cut(s) 3046
AjuI GAANNNNNNNTTGG 4 cut(s) 2740, 2772, 3024, 3056
AleI CACNNNNGTG 1 cut(s) 3249
Alw21I GWGCWC 4 cut(s) 37, 1085, 1857, 3194
Alw26I GTCTC 9 cut(s) 330, 480, 1333, 1716, 1718, 1765, 2107, 2288, 2612
Alw44I GTGCAC 3 cut(s) 1081, 1853, 3190
AlwI GGATC 1 cut(s) 3093
AlwNI CAGNNNCTG 4 cut(s) 211, 398, 779, 2279
Ama87I CYCGRG 1 cut(s) 650
AoxI GGCC 6 cut(s) 475, 800, 1380, 2877, 3049, 3314
ApaI GGGCCC 2 cut(s) 804, 1384
ApaLI GTGCAC 3 cut(s) 1081, 1853, 3190
ApeKI GCWGC 9 cut(s) 48, 51, 614, 1142, 1541, 2678, 2919, 2922, 3155
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
Asp700I GAANNNNTTC 3 cut(s) 604, 1030, 3026
AsuHPI GGTGA 9 cut(s) 405, 680, 968, 1754, 1766, 2686, 2846, 3088, 3225
AvaI CYCGRG 1 cut(s) 650
AvaII GGWCC 6 cut(s) 214, 535, 1061, 1128, 1322, 2733
AxyI CCTNAGG 1 cut(s) 1132
BaeGI GKGCMC 6 cut(s) 296, 804, 1085, 1384, 1857, 3194
BanI GGYRCC 2 cut(s) 293, 2056
BanII GRGCYC 3 cut(s) 37, 804, 1384
BarI GAAGNNNNNNTAC 2 cut(s) 1263, 1295
BbsI GAAGAC 4 cut(s) 241, 600, 2166, 2787
Bbv12I GWGCWC 4 cut(s) 37, 1085, 1857, 3194
BbvCI CCTCAGC 1 cut(s) 618
BbvI GCAGC 9 cut(s) 60, 63, 626, 1129, 1553, 2665, 2906, 2909, 3167
BccI CCATC 8 cut(s) 1313, 1399, 1403, 1785, 2105, 2473, 2852, 2950
BciT130I CCWGG 1 cut(s) 3048
BclI TGATCA 3 cut(s) 1048, 1744, 2836
BcoDI GTCTC 9 cut(s) 330, 480, 1333, 1716, 1718, 1765, 2107, 2288, 2612
BfaI CTAG 7 cut(s) 1305, 1524, 2064, 2813, 3000, 3309, 3318
BfmI CTRYAG 6 cut(s) 761, 1466, 2184, 2327, 2679, 2926
BfrI CTTAAG 1 cut(s) 91
BfuAI ACCTGC 2 cut(s) 1473, 1910
BglI GCCNNNNNGGC 1 cut(s) 3322
BglII AGATCT 1 cut(s) 2242
BmcAI AGTACT 1 cut(s) 2148
Bme1390I CCNGG 1 cut(s) 3048
Bme18I GGWCC 6 cut(s) 214, 535, 1061, 1128, 1322, 2733
BmeT110I CYCGRG 1 cut(s) 650
BmiI GGNNCC 9 cut(s) 295, 801, 802, 954, 1129, 1381, 1382, 1768, 2058
BmrFI CCNGG 1 cut(s) 3048
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 7 cut(s) 661, 791, 1303, 1528, 1906, 2659, 2819
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 4 cut(s) 241, 600, 2166, 2787
BpmI CTGGAG 2 cut(s) 2142, 2504
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 3 cut(s) 694, 1286, 1711
BsaBI GATNNNNATC 5 cut(s) 1665, 2115, 2202, 2367, 2442
BsaHI GRCGYC 1 cut(s) 2100
BsaI GGTCTC 3 cut(s) 480, 1333, 1716
BsaJI CCNNGG 4 cut(s) 297, 521, 651, 3046
BsaWI WCCGGW 1 cut(s) 1324
BsaXI ACNNNNNCTCC 2 cut(s) 851, 881
Bsc4I CCNNNNNNNGG 6 cut(s) 2096, 2186, 2552, 2775, 2990, 3005
Bse1I ACTGG 4 cut(s) 62, 409, 2154, 2478
Bse21I CCTNAGG 1 cut(s) 1132
Bse3DI GCAATG 4 cut(s) 1008, 1137, 1354, 2028
Bse8I GATNNNNATC 5 cut(s) 1665, 2115, 2202, 2367, 2442
BseBI CCWGG 1 cut(s) 3048
BseDI CCNNGG 4 cut(s) 297, 521, 651, 3046
BseJI GATNNNNATC 5 cut(s) 1665, 2115, 2202, 2367, 2442
BseLI CCNNNNNNNGG 6 cut(s) 2096, 2186, 2552, 2775, 2990, 3005
BseMI GCAATG 4 cut(s) 1008, 1137, 1354, 2028
BseNI ACTGG 4 cut(s) 62, 409, 2154, 2478
BseRI GAGGAG 1 cut(s) 3196
BseSI GKGCMC 6 cut(s) 296, 804, 1085, 1384, 1857, 3194
BseXI GCAGC 9 cut(s) 60, 63, 626, 1129, 1553, 2665, 2906, 2909, 3167
BseYI CCCAGC 1 cut(s) 1383
BshFI GGCC 6 cut(s) 477, 802, 1382, 2879, 3051, 3316
BshNI GGYRCC 2 cut(s) 293, 2056
BsiHKAI GWGCWC 4 cut(s) 37, 1085, 1857, 3194
BsiHKCI CYCGRG 1 cut(s) 650
BsiSI CCGG 1 cut(s) 1325
BslFI GGGAC 1 cut(s) 2568
BslI CCNNNNNNNGG 6 cut(s) 2096, 2186, 2552, 2775, 2990, 3005
BsmAI GTCTC 9 cut(s) 330, 480, 1333, 1716, 1718, 1765, 2107, 2288, 2612
BsmBI CGTCTC 1 cut(s) 2107
BsmFI GGGAC 1 cut(s) 2568
BsnI GGCC 6 cut(s) 477, 802, 1382, 2879, 3051, 3316
Bso31I GGTCTC 3 cut(s) 480, 1333, 1716
BsoBI CYCGRG 1 cut(s) 650
Bsp120I GGGCCC 2 cut(s) 800, 1380
Bsp1286I GDGCHC 7 cut(s) 37, 296, 804, 1085, 1384, 1857, 3194
BspACI CCGC 7 cut(s) 223, 310, 2832, 3207, 3228, 3313, 3349
BspANI GGCC 6 cut(s) 477, 802, 1382, 2879, 3051, 3316
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 9 cut(s) 295, 801, 802, 954, 1129, 1381, 1382, 1768, 2058
BspMAI CTGCAG 3 cut(s) 1470, 2331, 2683
BspMI ACCTGC 2 cut(s) 1473, 1910
BspPI GGATC 1 cut(s) 3093
BspT107I GGYRCC 2 cut(s) 293, 2056
BspTI CTTAAG 1 cut(s) 91
BspTNI GGTCTC 3 cut(s) 480, 1333, 1716
BsrDI GCAATG 4 cut(s) 1008, 1137, 1354, 2028
BsrI ACTGG 4 cut(s) 62, 409, 2154, 2478
BssECI CCNNGG 4 cut(s) 297, 521, 651, 3046
BssNI GRCGYC 1 cut(s) 2100
Bst2UI CCWGG 1 cut(s) 3048
Bst4CI ACNGT 9 cut(s) 491, 957, 1238, 1284, 1337, 1430, 1751, 1879, 2667
Bst6I CTCTTC 5 cut(s) 573, 2038, 2652, 2907, 3272
BstACI GRCGYC 1 cut(s) 2100
BstAFI CTTAAG 1 cut(s) 91
BstAPI GCANNNNNTGC 2 cut(s) 175, 1007
BstC8I GCNNGC 2 cut(s) 373, 451
BstEII GGTNACC 1 cut(s) 2692
BstENI CCTNNNNNAGG 1 cut(s) 3003
BstMAI GTCTC 9 cut(s) 330, 480, 1333, 1716, 1718, 1765, 2107, 2288, 2612
BstMWI GCNNNNNNNGC 9 cut(s) 175, 713, 815, 1007, 1193, 2266, 2587, 2789, 3322
BstNI CCWGG 1 cut(s) 3048
BstNSI RCATGY 4 cut(s) 375, 453, 1892, 3155
BstPI GGTNACC 1 cut(s) 2692
BstSCI CCNGG 1 cut(s) 3046
BstSFI CTRYAG 6 cut(s) 761, 1466, 2184, 2327, 2679, 2926
BstSLI GKGCMC 6 cut(s) 296, 804, 1085, 1384, 1857, 3194
BstV1I GCAGC 9 cut(s) 60, 63, 626, 1129, 1553, 2665, 2906, 2909, 3167
BstV2I GAAGAC 4 cut(s) 241, 600, 2166, 2787
BstX2I RGATCY 2 cut(s) 2242, 3085
BstYI RGATCY 2 cut(s) 2242, 3085
Bsu36I CCTNAGG 1 cut(s) 1132
BsuRI GGCC 6 cut(s) 477, 802, 1382, 2879, 3051, 3316
BtsI GCAGTG 2 cut(s) 112, 804
BtsIMutI CAGTG 4 cut(s) 112, 804, 1747, 3236
BveI ACCTGC 2 cut(s) 1473, 1910
Cac8I GCNNGC 2 cut(s) 373, 451
CaiI CAGNNNCTG 4 cut(s) 211, 398, 779, 2279
CciI TCATGA 1 cut(s) 1105
CseI GACGC 2 cut(s) 2089, 2781
Csp6I GTAC 3 cut(s) 1308, 2147, 2234
CviQI GTAC 3 cut(s) 1308, 2147, 2234
DraI TTTAAA 1 cut(s) 1833
Eam1104I CTCTTC 5 cut(s) 573, 2038, 2652, 2907, 3272
EarI CTCTTC 5 cut(s) 573, 2038, 2652, 2907, 3272
EciI GGCGGA 1 cut(s) 325
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 3 cut(s) 37, 804, 1384
Eco31I GGTCTC 3 cut(s) 480, 1333, 1716
Eco32I GATATC 1 cut(s) 1798
Eco47I GGWCC 6 cut(s) 214, 535, 1061, 1128, 1322, 2733
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 5 cut(s) 492, 630, 1006, 2781, 3074
Eco81I CCTNAGG 1 cut(s) 1132
Eco88I CYCGRG 1 cut(s) 650
Eco91I GGTNACC 1 cut(s) 2692
EcoICRI GAGCTC 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 3003
EcoO109I RGGNCCY 3 cut(s) 800, 1128, 1380
EcoO65I GGTNACC 1 cut(s) 2692
EcoRI GAATTC 2 cut(s) 10, 645
EcoRII CCWGG 1 cut(s) 3046
EcoRV GATATC 1 cut(s) 1798
EcoT22I ATGCAT 1 cut(s) 1164
EcoT38I GRGCYC 3 cut(s) 37, 804, 1384
Esp3I CGTCTC 1 cut(s) 2107
FalI AAGNNNNNCTT 2 cut(s) 1057, 1089
FaqI GGGAC 1 cut(s) 2568
FbaI TGATCA 3 cut(s) 1048, 1744, 2836
FblI GTMKAC 1 cut(s) 2995
FriOI GRGCYC 3 cut(s) 37, 804, 1384
FspBI CTAG 7 cut(s) 1305, 1524, 2064, 2813, 3000, 3309, 3318
GsaI CCCAGC 1 cut(s) 1387
GsuI CTGGAG 2 cut(s) 2142, 2504
HaeIII GGCC 6 cut(s) 477, 802, 1382, 2879, 3051, 3316
HapII CCGG 1 cut(s) 1325
HgaI GACGC 2 cut(s) 2089, 2781
Hin1I GRCGYC 1 cut(s) 2100
HincII GTYRAC 4 cut(s) 739, 1697, 2125, 2996
HindII GTYRAC 4 cut(s) 739, 1697, 2125, 2996
HindIII AAGCTT 3 cut(s) 816, 938, 1580
HpaII CCGG 1 cut(s) 1325
HphI GGTGA 9 cut(s) 405, 680, 968, 1754, 1766, 2686, 2846, 3088, 3225
Hpy99I CGWCG 1 cut(s) 991
HpyAV CCTTC 8 cut(s) 426, 467, 548, 1489, 2070, 3003, 3016, 3180
HpyCH4III ACNGT 9 cut(s) 491, 957, 1238, 1284, 1337, 1430, 1751, 1879, 2667
HpyF10VI GCNNNNNNNGC 9 cut(s) 175, 713, 815, 1007, 1193, 2266, 2587, 2789, 3322
Hsp92I GRCGYC 1 cut(s) 2100
Ksp22I TGATCA 3 cut(s) 1048, 1744, 2836
LmnI GCTCC 5 cut(s) 32, 781, 1070, 1772, 2161
Lsp1109I GCAGC 9 cut(s) 60, 63, 626, 1129, 1553, 2665, 2906, 2909, 3167
LweI GCATC 7 cut(s) 661, 791, 1303, 1528, 1906, 2659, 2819
MaeI CTAG 7 cut(s) 1305, 1524, 2064, 2813, 3000, 3309, 3318
MaeIII GTNAC 9 cut(s) 411, 1278, 1682, 1931, 2377, 2692, 2959, 3076, 3304
MfeI CAATTG 2 cut(s) 2613, 3263
MflI RGATCY 2 cut(s) 2242, 3085
MhlI GDGCHC 7 cut(s) 37, 296, 804, 1085, 1384, 1857, 3194
MlyI GAGTC 6 cut(s) 1702, 1720, 2053, 2180, 2507, 2909
MmeI TCCRAC 1 cut(s) 1392
Mph1103I ATGCAT 1 cut(s) 1164
MroXI GAANNNNTTC 3 cut(s) 604, 1030, 3026
MseI TTAA 8 cut(s) 92, 198, 743, 1148, 1253, 1422, 1832, 1908
MslI CAYNNNNRTG 5 cut(s) 418, 900, 1201, 2710, 3249
MspA1I CMGCKG 4 cut(s) 551, 617, 1568, 3209
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 1 cut(s) 1325
MspR9I CCNGG 1 cut(s) 3048
MunI CAATTG 2 cut(s) 2613, 3263
MvaI CCWGG 1 cut(s) 3048
MwoI GCNNNNNNNGC 9 cut(s) 175, 713, 815, 1007, 1193, 2266, 2587, 2789, 3322
NlaIV GGNNCC 9 cut(s) 295, 801, 802, 954, 1129, 1381, 1382, 1768, 2058
NmuCI GTSAC 7 cut(s) 411, 1682, 1931, 2377, 2692, 2959, 3076
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 4 cut(s) 375, 453, 1892, 3155
OliI CACNNNNGTG 1 cut(s) 3249
PaeI GCATGC 2 cut(s) 375, 453
PagI TCATGA 1 cut(s) 1105
PciI ACATGT 1 cut(s) 1888
PdmI GAANNNNTTC 3 cut(s) 604, 1030, 3026
PleI GAGTC 6 cut(s) 1701, 1720, 2052, 2179, 2507, 2908
PpsI GAGTC 6 cut(s) 1701, 1720, 2052, 2179, 2507, 2908
PpuMI RGGWCCY 1 cut(s) 1128
PscI ACATGT 1 cut(s) 1888
PsiI TTATAA 1 cut(s) 876
Psp124BI GAGCTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 1128
Psp6I CCWGG 1 cut(s) 3046
PspEI GGTNACC 1 cut(s) 2692
PspFI CCCAGC 1 cut(s) 1383
PspGI CCWGG 1 cut(s) 3046
PspN4I GGNNCC 9 cut(s) 295, 801, 802, 954, 1129, 1381, 1382, 1768, 2058
PspOMI GGGCCC 2 cut(s) 800, 1380
PspPPI RGGWCCY 1 cut(s) 1128
PstI CTGCAG 3 cut(s) 1470, 2331, 2683
PstNI CAGNNNCTG 4 cut(s) 211, 398, 779, 2279
PsuI RGATCY 2 cut(s) 2242, 3085
PvuII CAGCTG 3 cut(s) 551, 617, 1568
RsaI GTAC 3 cut(s) 1309, 2148, 2235
RsaNI GTAC 3 cut(s) 1308, 2147, 2234
RseI CAYNNNNRTG 5 cut(s) 418, 900, 1201, 2710, 3249
SacI GAGCTC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 2994
SaqAI TTAA 8 cut(s) 92, 198, 743, 1148, 1253, 1422, 1832, 1908
SbfI CCTGCAGG 1 cut(s) 1470
ScaI AGTACT 1 cut(s) 2148
SchI GAGTC 6 cut(s) 1702, 1720, 2053, 2180, 2507, 2909
ScrFI CCNGG 1 cut(s) 3048
SdaI CCTGCAGG 1 cut(s) 1470
SduI GDGCHC 7 cut(s) 37, 296, 804, 1085, 1384, 1857, 3194
SfaNI GCATC 7 cut(s) 661, 791, 1303, 1528, 1906, 2659, 2819
SfcI CTRYAG 6 cut(s) 761, 1466, 2184, 2327, 2679, 2926
SinI GGWCC 6 cut(s) 214, 535, 1061, 1128, 1322, 2733
SmiI ATTTAAAT 1 cut(s) 1833
SmiMI CAYNNNNRTG 5 cut(s) 418, 900, 1201, 2710, 3249
SmlI CTYRAG 4 cut(s) 91, 709, 1265, 1690
SmoI CTYRAG 4 cut(s) 91, 709, 1265, 1690
SphI GCATGC 2 cut(s) 375, 453
Sse8387I CCTGCAGG 1 cut(s) 1470
SsiI CCGC 7 cut(s) 223, 310, 2832, 3207, 3228, 3313, 3349
SspI AATATT 1 cut(s) 829
SspMI CTAG 7 cut(s) 1305, 1524, 2064, 2813, 3000, 3309, 3318
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 3046
SwaI ATTTAAAT 1 cut(s) 1833
TaaI ACNGT 9 cut(s) 491, 957, 1238, 1284, 1337, 1430, 1751, 1879, 2667
TaqI TCGA 5 cut(s) 541, 813, 989, 2247, 2995
TaqII GACCGA 1 cut(s) 1633
TatI WGTACW 3 cut(s) 1307, 2146, 2233
TauI GCSGC 3 cut(s) 3230, 3316, 3352
Tru1I TTAA 8 cut(s) 92, 198, 743, 1148, 1253, 1422, 1832, 1908
Tru9I TTAA 8 cut(s) 92, 198, 743, 1148, 1253, 1422, 1832, 1908
TscAI CASTG 4 cut(s) 112, 811, 1754, 3243
TseFI GTSAC 7 cut(s) 411, 1682, 1931, 2377, 2692, 2959, 3076
TseI GCWGC 9 cut(s) 48, 51, 614, 1142, 1541, 2678, 2919, 2922, 3155
Tsp45I GTSAC 7 cut(s) 411, 1682, 1931, 2377, 2692, 2959, 3076
TspGWI ACGGA 6 cut(s) 280, 612, 682, 1987, 2698, 3031
TspRI CASTG 4 cut(s) 112, 811, 1754, 3243
Vha464I CTTAAG 1 cut(s) 91
VneI GTGCAC 3 cut(s) 1081, 1853, 3190
VpaK11BI GGWCC 6 cut(s) 214, 535, 1061, 1128, 1322, 2733
XagI CCTNNNNNAGG 1 cut(s) 3003
XbaI TCTAGA 1 cut(s) 2812
XceI RCATGY 4 cut(s) 375, 453, 1892, 3155
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 2995
XmnI GAANNNNTTC 3 cut(s) 604, 1030, 3026
XspI CTAG 7 cut(s) 1305, 1524, 2064, 2813, 3000, 3309, 3318
ZrmI AGTACT 1 cut(s) 2148
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.