Rw7G004080

Plant protein of unknown function (DUF863)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003065
Physical Location & Seq
Forward (+)
3403967 .. 3409119
5153 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004080.1

Sequence Viewer

Length: 3462 bp
ATGTATCAGAATTCAGACTTGCTGGAAATGGGAGCTCATATTCCGTGCAGCAGCTATGCCCCAGTATATATCCCCACAATGAATCATAACTTAAGTGCTAATGGCAGTGAGAGGAATCATAATTTGACTTCTGACCAGCTTTTTTGGTATTACAAGGAGGTGTTCAAGCAGAAAATGCTCAATCAAGAAATGATATTTAAGAATCAGATTCTGGACCTCCACCGCCTTTATGGAAGACAAAGAGAACTAATGGATGATATAACACGGAATGAATTAGATAAACATAATCTGGGCACCGAGGTTTCAAGGCGGACTACTTCTTTGTCTCAGACTTCTCTTGTCTGTGCTCAAAAGACATTCAACATCCCTGCATGCAGTCAAGTATCTATTTCAGGTGCTGAAAATATCCAGTCACCTTCATGTTTTCTTCAAGGGAGGAACATACGGGCATGCTCTTATCCTGCTCAAACTGAAGGCCATTCAGGAGACCGTGAATTTTTGAAATCCAAATGCAAGAAACCTCGGAAGAACTTGGACCTTCGACTTCCAGCTGATACAATATACATTGATAGTGAAGAAGAGGAATTGTTAGAAGACGGAAAGGTTTCTGAAGCAGCTGAGGTATCAAGTTATCCTACTAAGAGAATTCCCGAGGTTGTGTGTAATGGTGATGCAACTAATGGATTCAACTCTGTTTCCAATTATGCCTCAAGTGCTTCATTAGAAAAAGGTTGGGTTGACTTAAATTATCTGTATAAGCTACGGAAAAAACAGCTCCTGCATCCGATAGTTTTGAGGGGCCCACTGCCGTCGAAGCTTACTCAAATATTCTACTGCAAGGCACAGAAAGCAAACAGGAGTGGGAAAATTCAAAGCAGCTTGTATTCTTTGCCTCGAGGATTTCATGCAGATGACATATCCTTGTCATCTAACTCATTACATAAGAAGCTTACGCAACAGGAACCGTCATCCTTTCTTCACAATCACCAAAATTCGTCGAGTGAAAGAGCAATGATTGCTGTTCAAAACTTCAGAAGAAATTCTGAAACTAAAAATGATCATCAATCTGGACCAAGTGGAGCTTTGATGTGCACATCATCCCAACATATTCCTCATGACTGCATGGAAAATTCTGGGTCCTCAGGCATTGCTGCTTTAAGAAAACCTATGCATGACTTGGCACGTACCCAGATTGCAGTTCAAGCACTCCCATGTCTTAACACCTCTATACCACTCAGTAATAACTGTAAATCATCAACATTAAGGCATGGGCTTGAGGAAGAAAGTTTACAGTTGAAGAAGCATCTGAGGTCTAGTACAAAACATGATGGTCCGGTCTCTGACAGTAGCTTTAGCAATGGCTTTGATTTAGAATCCAACAAATCAGGGGCCCAGCCACCATCCATCAGTTCTGATAATCTGATAAGGGTTAATGACGGTTTAGTGCCTGAACTTCATGGAATTACCAGATACCTGCAGGATTCTGCAAATGTCAATGCTTGGAAGGATATAGACTTGAATTTCATGCCTCCTAGCTGTTCTTCGGATGCAGCAGTTTCCCAGAGTTTACAAACAGCTGGAACAGAAAAGCTTGAAGATTCTAATGAGAGGTTTCCTTTGCATAGACCAAAACTTGATTATTATGGGAAAACTGATAAAGGATGTGCAGATTCAAATCAGGCAGAGGCAAGTGGCTTCTTGAGTCAACAGATTCATGGGTCTCCTACTTGTGAGACACCTAACATTTCTGGTGATCACTGTTATCATGTCTCAGGCTCCAATCACCATCAGAACTCAGTTCAAGATATCAAGGACAGCAAAAGAGCTGTGCTACTTGATTTAAATGTGCCTTGTGATTCTGTGCACAACAAAAAAGTAGAACTAACTGTTGATGAACATGTGGAAAAGAGTAAATTTAAGAAAGATGCAGGTTTTGGAGGTCACATTGACCTGAACTCATCCATAAATGATGTAGGTTTTGGAGTTCACATTGACCTGAACACATCCGTAAATGAAGATGAGTTTTCAGCAATGTCATCCTCTTCAACAGAGAGTCTTTTGGAGGCACCTGCTAGTCCAGAAAACAAAGAGTGTTCTCCACCGAGAGGCGTCTCATCTGATGGAAATCAAGTTGACACACCTGATCTAATGTCAGGACTGGAAGCTCCAGAAAACAAGGAGTCTTCTCCACCTACAGGAGAATCTGATGAAAATCATGTTGAGATACCTTTTCCATTGTCAGTACAGGAAGATCTCGAACATAAGGTGTGTTCTCAGCCAACAGAATCTGATGAAAAAGTTGATGCACCATCTCCATTGTCAGTACACGAAGATCTCGAACATAAGGTGTGTTCTAGGCCAACAGAATCTGATGAACAAATTGAGACACCTTTTGCATTGTCAGGGCAGGGAGGTCTGCAGAACAAAGAGTGTTCTCAGCCAATAGAATCTGATGAAAATCAAAGTGTGACACCTTTGCCATTGTCAAATATGGAAAGCAAAGATTGTTCTAAGCCTATGGATTCTGATGAAAATCAAATTGATACACCTTTTCCATCAGGACTGGATGATCTGGAGAGCAAAGATTGTTCTCAGCCTATGGACTCTGATGAAACTCAAATTCGGACACCTTTGCCATTGTCGGGACAGGAAAATCTGGAAACCAAAGAGCGTTCACAGCCAATGGATTCTACTGAAAAACAAATTGAGACACCTTTTCTATTGTCAGGACAGGAAGTGCATCTGGAAGAGGAACTGTTCAGGACTGCTGCAGAATGTTTGGTCACCATTTCATCATCCGTGTGTCATACTTGTTCTGAAAGGACCACATACAAGCCAACTGAAACTTCATCTGATTCCCTAAAGTGGTTTGCTGGTTTAGCGTCTTCAGTTTTAGGCAGTCTAGAAAACGAGGTGGGATGCGGTGATCATCAGGAGGTTCTACCTGATGGAATGGACTACTTTGAGGCCATGACATTGAAGCTACCAGAGTCAAAGGTGGAAGAGTGCTGCTGCTATAGAAGTAATGGCTACAAAGAGGAAGTGATGGTCACAGCTTCATCACAAAGTCAGCCAAAAAAGGGTCGACCTAGAAAAGGAAGGCAACGGAGGAAGGACTTCCAAAGGGAAATTCTCCCTGGCCTTGCTTCTTTGTCAAGGTGTGAGGTGACTGAGGATCTTCAGGTCATAGGAACACTAATTGAGGGTTCTGCTGATTGCTCCGAAACAAGATCAGTAAGACATGCAGCTAAATTTGGGTTGGCAAGGGGGAGGAGAAGGTGCACCATTTCTGCTTCCGCTGTAAAAGAGAATACTGCCGCTTCACCACTGAATCACATCGGTGTCAAAAGGCAATTGGAATGTGAGAAGAGAAGCCTAATTGATTGGGGAGAGGTAACTAGGCGGCGTAGAGGGCAGAGAAATTCGGGTAGTGATCTGCGGCTGATTAGAAACAAGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF863

Protein Analysis

1153

Amino Acids

127.59

Weight (kDa)

5.49

Isoelectric Point (pI)

61.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF863 PF05904 125 - 211 7e-10 Plant protein of unknown function (DUF863)
DUF863 PF05904 283 - 553 2.2e-12 Plant protein of unknown function (DUF863)
DUF863 PF05904 568 - 697 4.5e-08 Plant protein of unknown function (DUF863)
DUF863 PF05904 868 - 1140 8.4e-57 Plant protein of unknown function (DUF863)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2077
AbsI CCTCGAGG 1 cut(s) 894
Acc36I ACCTGC 3 cut(s) 1482, 1919, 2077
AccB1I GGYRCC 2 cut(s) 293, 2065
AccI GTMKAC 1 cut(s) 3085
AciI CCGC 7 cut(s) 223, 310, 2922, 3297, 3318, 3403, 3439
AclWI GGATC 1 cut(s) 3183
AcuI CTGAAG 5 cut(s) 492, 630, 1015, 2871, 3164
AcyI GRCGYC 1 cut(s) 2109
AfaI GTAC 4 cut(s) 1186, 1318, 2244, 2325
AfiI CCNNNNNNNGG 6 cut(s) 2105, 2195, 2642, 2865, 3080, 3095
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 1 cut(s) 1897
AjnI CCWGG 1 cut(s) 3136
AjuI GAANNNNNNNTTGG 4 cut(s) 2830, 2862, 3114, 3146
AleI CACNNNNGTG 1 cut(s) 3339
Alw21I GWGCWC 5 cut(s) 37, 349, 1094, 1866, 3284
Alw26I GTCTC 9 cut(s) 330, 480, 1342, 1725, 1727, 1774, 2116, 2378, 2702
Alw44I GTGCAC 3 cut(s) 1090, 1862, 3280
AlwI GGATC 1 cut(s) 3183
AlwNI CAGNNNCTG 5 cut(s) 211, 398, 778, 2288, 2369
Ama87I CYCGRG 2 cut(s) 650, 894
AoxI GGCC 6 cut(s) 475, 799, 1389, 2357, 2967, 3139
ApaI GGGCCC 2 cut(s) 803, 1393
ApaLI GTGCAC 3 cut(s) 1090, 1862, 3280
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
Asp700I GAANNNNTTC 3 cut(s) 604, 1039, 3116
AsuHPI GGTGA 9 cut(s) 405, 680, 977, 1763, 1775, 2776, 2936, 3178, 3315
AvaI CYCGRG 2 cut(s) 650, 894
AvaII GGWCC 6 cut(s) 214, 535, 1070, 1137, 1331, 2823
AxyI CCTNAGG 1 cut(s) 1141
BaeGI GKGCMC 6 cut(s) 296, 803, 1094, 1393, 1866, 3284
BanI GGYRCC 2 cut(s) 293, 2065
BanII GRGCYC 3 cut(s) 37, 803, 1393
BarI GAAGNNNNNNTAC 2 cut(s) 1272, 1304
BbsI GAAGAC 4 cut(s) 241, 600, 2175, 2877
Bbv12I GWGCWC 5 cut(s) 37, 349, 1094, 1866, 3284
BbvCI CCTCAGC 1 cut(s) 618
BccI CCATC 9 cut(s) 1322, 1408, 1412, 1794, 2114, 2317, 2563, 2942, 3040
BceAI ACGGC 1 cut(s) 793
BciT130I CCWGG 1 cut(s) 3138
BclI TGATCA 3 cut(s) 1057, 1753, 2926
BcoDI GTCTC 9 cut(s) 330, 480, 1342, 1725, 1727, 1774, 2116, 2378, 2702
BfaI CTAG 7 cut(s) 1314, 1533, 2073, 2355, 2903, 3090, 3399
BfmI CTRYAG 5 cut(s) 1475, 2193, 2417, 2769, 3016
BfrI CTTAAG 1 cut(s) 91
BfuAI ACCTGC 3 cut(s) 1482, 1919, 2077
BglII AGATCT 2 cut(s) 2251, 2332
Bme1390I CCNGG 1 cut(s) 3138
Bme18I GGWCC 6 cut(s) 214, 535, 1070, 1137, 1331, 2823
BmeT110I CYCGRG 2 cut(s) 650, 894
BmiI GGNNCC 9 cut(s) 295, 800, 801, 963, 1138, 1390, 1391, 1777, 2067
BmrFI CCNGG 1 cut(s) 3138
BmrI ACTGGG 1 cut(s) 56
BmsI GCATC 8 cut(s) 661, 790, 1312, 1537, 1915, 2293, 2749, 2909
BmuI ACTGGG 1 cut(s) 56
BpiI GAAGAC 4 cut(s) 241, 600, 2175, 2877
BpmI CTGGAG 2 cut(s) 2151, 2594
Bpu10I CCTNAGC 1 cut(s) 618
BpuEI CTTGAG 3 cut(s) 694, 1295, 1720
BsaAI YACGTR 1 cut(s) 1184
BsaBI GATNNNNATC 5 cut(s) 1674, 2124, 2211, 2457, 2532
BsaHI GRCGYC 1 cut(s) 2109
BsaI GGTCTC 3 cut(s) 480, 1342, 1725
BsaJI CCNNGG 4 cut(s) 297, 521, 651, 3136
BsaWI WCCGGW 1 cut(s) 1333
Bsc4I CCNNNNNNNGG 6 cut(s) 2105, 2195, 2642, 2865, 3080, 3095
Bse1I ACTGG 4 cut(s) 62, 409, 2163, 2568
Bse21I CCTNAGG 1 cut(s) 1141
Bse3DI GCAATG 4 cut(s) 1017, 1146, 1363, 2037
Bse8I GATNNNNATC 5 cut(s) 1674, 2124, 2211, 2457, 2532
BseBI CCWGG 1 cut(s) 3138
BseDI CCNNGG 4 cut(s) 297, 521, 651, 3136
BseJI GATNNNNATC 5 cut(s) 1674, 2124, 2211, 2457, 2532
BseLI CCNNNNNNNGG 6 cut(s) 2105, 2195, 2642, 2865, 3080, 3095
BseMI GCAATG 4 cut(s) 1017, 1146, 1363, 2037
BseNI ACTGG 4 cut(s) 62, 409, 2163, 2568
BseRI GAGGAG 1 cut(s) 3286
BseSI GKGCMC 6 cut(s) 296, 803, 1094, 1393, 1866, 3284
BseYI CCCAGC 1 cut(s) 1392
BsgI GTGCAG 2 cut(s) 67, 1686
BshFI GGCC 6 cut(s) 477, 801, 1391, 2359, 2969, 3141
BshNI GGYRCC 2 cut(s) 293, 2065
BsiHKAI GWGCWC 5 cut(s) 37, 349, 1094, 1866, 3284
BsiHKCI CYCGRG 2 cut(s) 650, 894
BsiSI CCGG 1 cut(s) 1334
BslFI GGGAC 1 cut(s) 2658
BslI CCNNNNNNNGG 6 cut(s) 2105, 2195, 2642, 2865, 3080, 3095
BsmAI GTCTC 9 cut(s) 330, 480, 1342, 1725, 1727, 1774, 2116, 2378, 2702
BsmBI CGTCTC 1 cut(s) 2116
BsmFI GGGAC 1 cut(s) 2658
BsnI GGCC 6 cut(s) 477, 801, 1391, 2359, 2969, 3141
Bso31I GGTCTC 3 cut(s) 480, 1342, 1725
BsoBI CYCGRG 2 cut(s) 650, 894
Bsp120I GGGCCC 2 cut(s) 799, 1389
Bsp1286I GDGCHC 8 cut(s) 37, 296, 349, 803, 1094, 1393, 1866, 3284
BspACI CCGC 7 cut(s) 223, 310, 2922, 3297, 3318, 3403, 3439
BspANI GGCC 6 cut(s) 477, 801, 1391, 2359, 2969, 3141
BspHI TCATGA 1 cut(s) 1114
BspLI GGNNCC 9 cut(s) 295, 800, 801, 963, 1138, 1390, 1391, 1777, 2067
BspMAI CTGCAG 3 cut(s) 1479, 2421, 2773
BspMI ACCTGC 3 cut(s) 1482, 1919, 2077
BspPI GGATC 1 cut(s) 3183
BspT107I GGYRCC 2 cut(s) 293, 2065
BspTI CTTAAG 1 cut(s) 91
BspTNI GGTCTC 3 cut(s) 480, 1342, 1725
BsrDI GCAATG 4 cut(s) 1017, 1146, 1363, 2037
BsrI ACTGG 4 cut(s) 62, 409, 2163, 2568
BssECI CCNNGG 4 cut(s) 297, 521, 651, 3136
BssNI GRCGYC 1 cut(s) 2109
Bst2UI CCWGG 1 cut(s) 3138
Bst4CI ACNGT 9 cut(s) 491, 966, 1247, 1293, 1346, 1439, 1760, 1888, 2757
Bst6I CTCTTC 5 cut(s) 573, 2047, 2742, 2997, 3362
BstACI GRCGYC 1 cut(s) 2109
BstAFI CTTAAG 1 cut(s) 91
BstAPI GCANNNNNTGC 2 cut(s) 175, 1016
BstBAI YACGTR 1 cut(s) 1184
BstC8I GCNNGC 2 cut(s) 373, 451
BstEII GGTNACC 1 cut(s) 2782
BstENI CCTNNNNNAGG 1 cut(s) 3093
BstMAI GTCTC 9 cut(s) 330, 480, 1342, 1725, 1727, 1774, 2116, 2378, 2702
BstMWI GCNNNNNNNGC 9 cut(s) 175, 713, 814, 848, 1016, 1202, 2677, 2879, 3412
BstNI CCWGG 1 cut(s) 3138
BstNSI RCATGY 4 cut(s) 375, 453, 1901, 3245
BstPI GGTNACC 1 cut(s) 2782
BstSCI CCNGG 1 cut(s) 3136
BstSFI CTRYAG 5 cut(s) 1475, 2193, 2417, 2769, 3016
BstSLI GKGCMC 6 cut(s) 296, 803, 1094, 1393, 1866, 3284
BstV2I GAAGAC 4 cut(s) 241, 600, 2175, 2877
BstX2I RGATCY 3 cut(s) 2251, 2332, 3175
BstYI RGATCY 3 cut(s) 2251, 2332, 3175
Bsu36I CCTNAGG 1 cut(s) 1141
BsuRI GGCC 6 cut(s) 477, 801, 1391, 2359, 2969, 3141
BtsI GCAGTG 2 cut(s) 112, 803
BtsIMutI CAGTG 4 cut(s) 112, 803, 1756, 3326
BveI ACCTGC 3 cut(s) 1482, 1919, 2077
Cac8I GCNNGC 2 cut(s) 373, 451
CaiI CAGNNNCTG 5 cut(s) 211, 398, 778, 2288, 2369
CciI TCATGA 1 cut(s) 1114
CseI GACGC 2 cut(s) 2098, 2871
Csp6I GTAC 4 cut(s) 1185, 1317, 2243, 2324
CviQI GTAC 4 cut(s) 1185, 1317, 2243, 2324
DraI TTTAAA 1 cut(s) 1842
Eam1104I CTCTTC 5 cut(s) 573, 2047, 2742, 2997, 3362
EarI CTCTTC 5 cut(s) 573, 2047, 2742, 2997, 3362
EciI GGCGGA 1 cut(s) 325
Ecl136II GAGCTC 1 cut(s) 35
Eco24I GRGCYC 3 cut(s) 37, 803, 1393
Eco31I GGTCTC 3 cut(s) 480, 1342, 1725
Eco32I GATATC 1 cut(s) 1807
Eco47I GGWCC 6 cut(s) 214, 535, 1070, 1137, 1331, 2823
Eco53kI GAGCTC 1 cut(s) 35
Eco57I CTGAAG 5 cut(s) 492, 630, 1015, 2871, 3164
Eco81I CCTNAGG 1 cut(s) 1141
Eco88I CYCGRG 2 cut(s) 650, 894
Eco91I GGTNACC 1 cut(s) 2782
EcoICRI GAGCTC 1 cut(s) 35
EcoNI CCTNNNNNAGG 1 cut(s) 3093
EcoO109I RGGNCCY 3 cut(s) 799, 1137, 1389
EcoO65I GGTNACC 1 cut(s) 2782
EcoRI GAATTC 2 cut(s) 10, 645
EcoRII CCWGG 1 cut(s) 3136
EcoRV GATATC 1 cut(s) 1807
EcoT22I ATGCAT 1 cut(s) 1173
EcoT38I GRGCYC 3 cut(s) 37, 803, 1393
Esp3I CGTCTC 1 cut(s) 2116
FalI AAGNNNNNCTT 2 cut(s) 1066, 1098
FaqI GGGAC 1 cut(s) 2658
FbaI TGATCA 3 cut(s) 1057, 1753, 2926
FblI GTMKAC 1 cut(s) 3085
FriOI GRGCYC 3 cut(s) 37, 803, 1393
FspBI CTAG 7 cut(s) 1314, 1533, 2073, 2355, 2903, 3090, 3399
GsaI CCCAGC 1 cut(s) 1396
GsuI CTGGAG 2 cut(s) 2151, 2594
HaeIII GGCC 6 cut(s) 477, 801, 1391, 2359, 2969, 3141
HapII CCGG 1 cut(s) 1334
HgaI GACGC 2 cut(s) 2098, 2871
Hin1I GRCGYC 1 cut(s) 2109
HincII GTYRAC 4 cut(s) 739, 1706, 2134, 3086
HindII GTYRAC 4 cut(s) 739, 1706, 2134, 3086
HindIII AAGCTT 3 cut(s) 815, 947, 1589
HpaII CCGG 1 cut(s) 1334
HphI GGTGA 9 cut(s) 405, 680, 977, 1763, 1775, 2776, 2936, 3178, 3315
Hpy99I CGWCG 2 cut(s) 814, 1000
HpyAV CCTTC 7 cut(s) 426, 467, 548, 1498, 3093, 3106, 3270
HpyCH4III ACNGT 9 cut(s) 491, 966, 1247, 1293, 1346, 1439, 1760, 1888, 2757
HpyCH4IV ACGT 1 cut(s) 1183
HpyF10VI GCNNNNNNNGC 9 cut(s) 175, 713, 814, 848, 1016, 1202, 2677, 2879, 3412
HpySE526I ACGT 1 cut(s) 1183
Hsp92I GRCGYC 1 cut(s) 2109
Ksp22I TGATCA 3 cut(s) 1057, 1753, 2926
LmnI GCTCC 6 cut(s) 32, 780, 1079, 1781, 2170, 3224
LweI GCATC 8 cut(s) 661, 790, 1312, 1537, 1915, 2293, 2749, 2909
MaeI CTAG 7 cut(s) 1314, 1533, 2073, 2355, 2903, 3090, 3399
MaeII ACGT 1 cut(s) 1183
MaeIII GTNAC 7 cut(s) 411, 1940, 2467, 2782, 3049, 3166, 3394
MfeI CAATTG 1 cut(s) 3353
MflI RGATCY 3 cut(s) 2251, 2332, 3175
MhlI GDGCHC 8 cut(s) 37, 296, 349, 803, 1094, 1393, 1866, 3284
MlyI GAGTC 5 cut(s) 1711, 2062, 2189, 2597, 2999
MmeI TCCRAC 1 cut(s) 1401
Mph1103I ATGCAT 1 cut(s) 1173
MroXI GAANNNNTTC 3 cut(s) 604, 1039, 3116
MseI TTAA 9 cut(s) 92, 198, 743, 1157, 1218, 1262, 1431, 1841, 1917
MslI CAYNNNNRTG 5 cut(s) 418, 909, 1210, 2800, 3339
MspA1I CMGCKG 4 cut(s) 551, 617, 1577, 3299
MspCI CTTAAG 1 cut(s) 91
MspI CCGG 1 cut(s) 1334
MspR9I CCNGG 1 cut(s) 3138
MunI CAATTG 1 cut(s) 3353
MvaI CCWGG 1 cut(s) 3138
MwoI GCNNNNNNNGC 9 cut(s) 175, 713, 814, 848, 1016, 1202, 2677, 2879, 3412
NlaIV GGNNCC 9 cut(s) 295, 800, 801, 963, 1138, 1390, 1391, 1777, 2067
NmuCI GTSAC 6 cut(s) 411, 1940, 2467, 2782, 3049, 3166
NsiI ATGCAT 1 cut(s) 1173
NspI RCATGY 4 cut(s) 375, 453, 1901, 3245
OliI CACNNNNGTG 1 cut(s) 3339
PaeI GCATGC 2 cut(s) 375, 453
PaeR7I CTCGAG 1 cut(s) 894
PagI TCATGA 1 cut(s) 1114
PaqCI CACCTGC 1 cut(s) 2077
PciI ACATGT 1 cut(s) 1897
PcsI WCGNNNNNNNCGW 1 cut(s) 2334
PdmI GAANNNNTTC 3 cut(s) 604, 1039, 3116
PleI GAGTC 5 cut(s) 1710, 2061, 2188, 2597, 2998
PpsI GAGTC 5 cut(s) 1710, 2061, 2188, 2597, 2998
Ppu21I YACGTR 1 cut(s) 1184
PpuMI RGGWCCY 1 cut(s) 1137
PscI ACATGT 1 cut(s) 1897
Psp124BI GAGCTC 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 1137
Psp6I CCWGG 1 cut(s) 3136
PspEI GGTNACC 1 cut(s) 2782
PspFI CCCAGC 1 cut(s) 1392
PspGI CCWGG 1 cut(s) 3136
PspN4I GGNNCC 9 cut(s) 295, 800, 801, 963, 1138, 1390, 1391, 1777, 2067
PspOMI GGGCCC 2 cut(s) 799, 1389
PspPPI RGGWCCY 1 cut(s) 1137
PspXI VCTCGAGB 1 cut(s) 894
PstI CTGCAG 3 cut(s) 1479, 2421, 2773
PstNI CAGNNNCTG 5 cut(s) 211, 398, 778, 2288, 2369
PsuI RGATCY 3 cut(s) 2251, 2332, 3175
PvuII CAGCTG 3 cut(s) 551, 617, 1577
RsaI GTAC 4 cut(s) 1186, 1318, 2244, 2325
RsaNI GTAC 4 cut(s) 1185, 1317, 2243, 2324
RseI CAYNNNNRTG 5 cut(s) 418, 909, 1210, 2800, 3339
SacI GAGCTC 1 cut(s) 37
SalI GTCGAC 1 cut(s) 3084
SaqAI TTAA 9 cut(s) 92, 198, 743, 1157, 1218, 1262, 1431, 1841, 1917
SbfI CCTGCAGG 1 cut(s) 1479
SchI GAGTC 5 cut(s) 1711, 2062, 2189, 2597, 2999
ScrFI CCNGG 1 cut(s) 3138
SdaI CCTGCAGG 1 cut(s) 1479
SduI GDGCHC 8 cut(s) 37, 296, 349, 803, 1094, 1393, 1866, 3284
SfaNI GCATC 8 cut(s) 661, 790, 1312, 1537, 1915, 2293, 2749, 2909
SfcI CTRYAG 5 cut(s) 1475, 2193, 2417, 2769, 3016
Sfr274I CTCGAG 1 cut(s) 894
SinI GGWCC 6 cut(s) 214, 535, 1070, 1137, 1331, 2823
SlaI CTCGAG 1 cut(s) 894
SmiI ATTTAAAT 1 cut(s) 1842
SmiMI CAYNNNNRTG 5 cut(s) 418, 909, 1210, 2800, 3339
SmlI CTYRAG 5 cut(s) 91, 709, 894, 1274, 1699
SmoI CTYRAG 5 cut(s) 91, 709, 894, 1274, 1699
SphI GCATGC 2 cut(s) 375, 453
Sse8387I CCTGCAGG 1 cut(s) 1479
SsiI CCGC 7 cut(s) 223, 310, 2922, 3297, 3318, 3403, 3439
SspI AATATT 1 cut(s) 828
SspMI CTAG 7 cut(s) 1314, 1533, 2073, 2355, 2903, 3090, 3399
SstI GAGCTC 1 cut(s) 37
StyD4I CCNGG 1 cut(s) 3136
SwaI ATTTAAAT 1 cut(s) 1842
TaaI ACNGT 9 cut(s) 491, 966, 1247, 1293, 1346, 1439, 1760, 1888, 2757
TaiI ACGT 1 cut(s) 1186
TaqI TCGA 7 cut(s) 541, 812, 895, 998, 2256, 2337, 3085
TatI WGTACW 3 cut(s) 1316, 2242, 2323
TauI GCSGC 3 cut(s) 3320, 3406, 3442
Tru1I TTAA 9 cut(s) 92, 198, 743, 1157, 1218, 1262, 1431, 1841, 1917
Tru9I TTAA 9 cut(s) 92, 198, 743, 1157, 1218, 1262, 1431, 1841, 1917
TscAI CASTG 4 cut(s) 112, 810, 1763, 3333
TseFI GTSAC 6 cut(s) 411, 1940, 2467, 2782, 3049, 3166
Tsp45I GTSAC 6 cut(s) 411, 1940, 2467, 2782, 3049, 3166
TspGWI ACGGA 7 cut(s) 33, 280, 612, 778, 1996, 2788, 3121
TspRI CASTG 4 cut(s) 112, 810, 1763, 3333
Vha464I CTTAAG 1 cut(s) 91
VneI GTGCAC 3 cut(s) 1090, 1862, 3280
VpaK11BI GGWCC 6 cut(s) 214, 535, 1070, 1137, 1331, 2823
XagI CCTNNNNNAGG 1 cut(s) 3093
XbaI TCTAGA 1 cut(s) 2902
XceI RCATGY 4 cut(s) 375, 453, 1901, 3245
XcmI CCANNNNNNNNNTGG 1 cut(s) 227
XhoI CTCGAG 1 cut(s) 894
XmiI GTMKAC 1 cut(s) 3085
XmnI GAANNNNTTC 3 cut(s) 604, 1039, 3116
XspI CTAG 7 cut(s) 1314, 1533, 2073, 2355, 2903, 3090, 3399
Zsp2I ATGCAT 1 cut(s) 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.