Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005398

Physical Location & Seq

Genomic Coordinates Reverse (-)

29544984 .. 29548877

Gene Structure 3894 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1209200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 786 bp

ATGGGTTGCAATACAAGCAAAAACGTGGATTCGAAAGCAAGTCGGGCCGCCCGGTGGCGATCCACCGGCATTGTTGGCCTCCGTGATTCCAAATTGAAGACATTTCCAGATGAAGTTCTTGACCTGGACAAGTCCATACGTACTCTTGATCTAACACACAATAAAATAGTTGACATTCCTGTGGAGATCAGCAAATTAGCAAACCTGCAGCGACTGATATTAGCTCATAACCTCATTGACCGACTGCCGATAAATTTGGCAAAACTAGTGTCCCTAAAAGTTATGACACTTGATGGAAATCGAGTTACTACCTTGCCTGATGAATTGGGACAGTTGGTCAGACTTGAGCAGTTATCTATCTCTGGGAATTCATTAGAGTCATTGCCTGAGACTATTGGAAGCTTGCGGAATTTAAAACTTTTAAATTTATCGAAGAACAAATTAAAGTCTCTTCCAGAATCCATAGGAAGTTGCTTCTCTCTGGAAGAATTACAAGCAAATGATAATTCAATTGAAGAACTTCCTGAATCAGTTTGCAACCTTATCCACGTAAAGTCACTTTGCTTGGACAATAATAATGTGAAACAGATACCCACTAAGCTGTTGAAAGACTGTAAAGCGCTGCAGAATATTTCCCTTCATGGAAACCCTATTTCCATGGATCAGTTTCAGCAGATGGAAGGATTCCAAGAGTTTGAAGCGAGGAGGAAGAAGAAGTTCGACAAACAAATTGACTCAAATGTGATGATCAGTTCGAAAGGCCTTGATGAGGGCGTTGATCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04014 map04014

Pfam Domains

Protein Families

LRR_8

Protein Analysis

262

Amino Acids

29.11

Weight (kDa)

7.62

Isoelectric Point (pI)

40.47

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	26 - 79	2e-06	Leucine rich repeat
LRR_14	PF23598	26 - 96	6.4e-06	Leucine-rich repeat region
LRR_8	PF13855	45 - 101	1.6e-09	Leucine rich repeat
LRR_8	PF13855	68 - 125	4.6e-08	Leucine rich repeat
LRR_14	PF23598	85 - 165	4.2e-13	Leucine-rich repeat region
LRR_8	PF13855	114 - 171	1.6e-07	Leucine rich repeat
LRR_14	PF23598	131 - 245	2.9e-12	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005398)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07910
fragaria_vesca	FvH4_5g14250 FvH4_5g14250 FvH4_5g14250
malus_domestica	MD06G1200000.v1.1 MD14G1209200.v1.1
prunus_persica	Prupe.5G201600_v2.0.a1 Prupe.5G201600_v2.0.a1 Prupe.5G201600_v2.0.a1 Prupe.5G201600_v2.0.a1 Prupe.5G201600_v2.0.a1 Prupe.5G201600_v2.0.a1
pyrus_communis	pycom06g17670 pycom14g17300
rosa_chinensis	RchiOBHm_Chr7g0182671
rosa_laevigata	RLG00000005110
rosa_multiflora	Rmu_co8261001.1_g000001 Rmu_sc0038024.1_g000001 Rmu_sc0038137.1_g000001
rosa_roxburghii	Rroxscaffold_3G00271080
rosa_rugosa	Rorug06G0448900 Rorug06G0449000 Rorug06G0449000 Rorug06G0449000
rosa_samantha	Rh7AG052800 Rh7BG052400 Rh7CG053900 Rh7DG052200
rosa_wichuraiana	Rw7G004300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	213
AciI	CCGC	2 cut(s)	48, 406
AclWI	GGATC	2 cut(s)	54, 669
AcsI	RAATTY	4 cut(s)	253, 367, 409, 424
AfaI	GTAC	1 cut(s)	142
AfeI	AGCGCT	1 cut(s)	621
AfiI	CCNNNNNNNGG	2 cut(s)	54, 769
AgsI	TTSAA	5 cut(s)	97, 510, 515, 607, 698
AhdI	GACNNNNNGTC	1 cut(s)	335
AhlI	ACTAGT	1 cut(s)	265
AjnI	CCWGG	1 cut(s)	123
AluBI	AGCT	3 cut(s)	224, 402, 601
AluI	AGCT	3 cut(s)	224, 402, 601
Alw26I	GTCTC	2 cut(s)	383, 453
AlwI	GGATC	2 cut(s)	54, 669
AlwNI	CAGNNNCTG	1 cut(s)	214
Aor51HI	AGCGCT	1 cut(s)	621
AoxI	GGCC	3 cut(s)	45, 76, 760
ApeKI	GCWGC	2 cut(s)	208, 622
ApoI	RAATTY	4 cut(s)	253, 367, 409, 424
ArsI	GACNNNNNNTTYG	2 cut(s)	254, 286
Asp700I	GAANNNNTTC	2 cut(s)	519, 716
AspLEI	GCGC	1 cut(s)	622
AspS9I	GGNCC	1 cut(s)	45
AsuC2I	CCSGG	1 cut(s)	52
AsuII	TTCGAA	2 cut(s)	32, 755
BbsI	GAAGAC	1 cut(s)	104
BbvI	GCAGC	2 cut(s)	220, 609
BccI	CCATC	2 cut(s)	287, 670
BciT130I	CCWGG	1 cut(s)	125
BclI	TGATCA	1 cut(s)	747
BcnI	CCSGG	1 cut(s)	52
BcoDI	GTCTC	2 cut(s)	383, 453
BcuI	ACTAGT	1 cut(s)	265
BfaI	CTAG	1 cut(s)	266
BfmI	CTRYAG	2 cut(s)	206, 623
BfoI	RGCGCY	1 cut(s)	623
BfuAI	ACCTGC	1 cut(s)	213
BisI	GCNGC	3 cut(s)	48, 209, 623
BlsI	GCNGC	3 cut(s)	49, 210, 624
Bme1390I	CCNGG	2 cut(s)	52, 125
BmeRI	GACNNNNNGTC	1 cut(s)	335
BmgT120I	GGNCC	1 cut(s)	45
BmrFI	CCNGG	2 cut(s)	52, 125
BpiI	GAAGAC	1 cut(s)	104
Bpu14I	TTCGAA	2 cut(s)	32, 755
BpuEI	CTTGAG	1 cut(s)	365
BpuMI	CCSGG	1 cut(s)	52
BsaAI	YACGTR	2 cut(s)	140, 550
BsaBI	GATNNNNATC	1 cut(s)	297
BsaJI	CCNNGG	1 cut(s)	657
Bsc4I	CCNNNNNNNGG	2 cut(s)	54, 769
Bse118I	RCCGGY	1 cut(s)	65
Bse3DI	GCAATG	1 cut(s)	380
Bse8I	GATNNNNATC	1 cut(s)	297
BseBI	CCWGG	1 cut(s)	125
BseDI	CCNNGG	1 cut(s)	657
BseJI	GATNNNNATC	1 cut(s)	297
BseLI	CCNNNNNNNGG	2 cut(s)	54, 769
BseMI	GCAATG	1 cut(s)	380
BseMII	CTCAG	1 cut(s)	378
BseRI	GAGGAG	1 cut(s)	718
BseXI	GCAGC	2 cut(s)	220, 609
BshFI	GGCC	3 cut(s)	47, 78, 762
BsiSI	CCGG	2 cut(s)	52, 66
BslFI	GGGAC	2 cut(s)	256, 342
BslI	CCNNNNNNNGG	2 cut(s)	54, 769
BsmAI	GTCTC	2 cut(s)	383, 453
BsmFI	GGGAC	2 cut(s)	256, 342
BsnI	GGCC	3 cut(s)	47, 78, 762
Bsp119I	TTCGAA	2 cut(s)	32, 755
Bsp143I	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
Bsp19I	CCATGG	1 cut(s)	657
BspACI	CCGC	2 cut(s)	48, 406
BspANI	GGCC	3 cut(s)	47, 78, 762
BspCNI	CTCAG	1 cut(s)	379
BspMAI	CTGCAG	2 cut(s)	210, 627
BspMI	ACCTGC	1 cut(s)	213
BspPI	GGATC	2 cut(s)	54, 669
BspT104I	TTCGAA	2 cut(s)	32, 755
BsrDI	GCAATG	1 cut(s)	380
BsrFI	RCCGGY	1 cut(s)	65
BssAI	RCCGGY	1 cut(s)	65
BssECI	CCNNGG	1 cut(s)	657
BssMI	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
BssT1I	CCWWGG	1 cut(s)	657
Bst2UI	CCWGG	1 cut(s)	125
Bst4CI	ACNGT	2 cut(s)	333, 614
Bst6I	CTCTTC	1 cut(s)	456
BstBAI	YACGTR	2 cut(s)	140, 550
BstBI	TTCGAA	2 cut(s)	32, 755
BstC8I	GCNNGC	1 cut(s)	404
BstDEI	CTNAG	2 cut(s)	387, 597
BstDSI	CCRYGG	1 cut(s)	657
BstENI	CCTNNNNNAGG	1 cut(s)	767
BstH2I	RGCGCY	1 cut(s)	623
BstHHI	GCGC	1 cut(s)	622
BstKTI	GATC	6 cut(s)	62, 151, 189, 664, 750, 781
BstMAI	GTCTC	2 cut(s)	383, 453
BstMBI	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
BstMWI	GCNNNNNNNGC	3 cut(s)	15, 44, 75
BstNI	CCWGG	1 cut(s)	125
BstSCI	CCNGG	2 cut(s)	50, 123
BstSFI	CTRYAG	2 cut(s)	206, 623
BstSNI	TACGTA	1 cut(s)	140
BstV1I	GCAGC	2 cut(s)	220, 609
BstV2I	GAAGAC	1 cut(s)	104
BsuRI	GGCC	3 cut(s)	47, 78, 762
BtgI	CCRYGG	1 cut(s)	657
BveI	ACCTGC	1 cut(s)	213
Cac8I	GCNNGC	1 cut(s)	404
CaiI	CAGNNNCTG	1 cut(s)	214
CfoI	GCGC	1 cut(s)	622
Cfr10I	RCCGGY	1 cut(s)	65
Cfr13I	GGNCC	1 cut(s)	45
Csp6I	GTAC	1 cut(s)	141
CspCI	CAANNNNNGTGG	2 cut(s)	52, 87
CviAII	CATG	2 cut(s)	641, 658
CviJI	RGCY	6 cut(s)	47, 78, 224, 402, 601, 762
CviKI_1	RGCY	6 cut(s)	47, 78, 224, 402, 601, 762
CviQI	GTAC	1 cut(s)	141
DdeI	CTNAG	2 cut(s)	387, 597
DpnI	GATC	6 cut(s)	61, 150, 188, 663, 749, 780
DpnII	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
DraI	TTTAAA	2 cut(s)	414, 423
DriI	GACNNNNNGTC	1 cut(s)	335
Eam1104I	CTCTTC	1 cut(s)	456
Eam1105I	GACNNNNNGTC	1 cut(s)	335
EarI	CTCTTC	1 cut(s)	456
Eco105I	TACGTA	1 cut(s)	140
Eco130I	CCWWGG	1 cut(s)	657
Eco147I	AGGCCT	1 cut(s)	762
Eco47III	AGCGCT	1 cut(s)	621
EcoNI	CCTNNNNNAGG	1 cut(s)	767
EcoRI	GAATTC	1 cut(s)	367
EcoRII	CCWGG	1 cut(s)	123
EcoT14I	CCWWGG	1 cut(s)	657
ErhI	CCWWGG	1 cut(s)	657
FaeI	CATG	2 cut(s)	644, 661
FaiI	YATR	6 cut(s)	137, 228, 284, 464, 642, 659
FaqI	GGGAC	2 cut(s)	256, 342
FatI	CATG	2 cut(s)	640, 657
FbaI	TGATCA	1 cut(s)	747
Fnu4HI	GCNGC	3 cut(s)	48, 209, 623
Fsp4HI	GCNGC	3 cut(s)	48, 209, 623
FspBI	CTAG	1 cut(s)	266
GlaI	GCGC	1 cut(s)	621
GluI	GCNGC	3 cut(s)	48, 209, 623
HaeII	RGCGCY	1 cut(s)	623
HaeIII	GGCC	3 cut(s)	47, 78, 762
HapII	CCGG	2 cut(s)	52, 66
HhaI	GCGC	1 cut(s)	622
Hin1II	CATG	2 cut(s)	644, 661
Hin6I	GCGC	1 cut(s)	620
HinP1I	GCGC	1 cut(s)	620
HincII	GTYRAC	1 cut(s)	172
HindII	GTYRAC	1 cut(s)	172
HindIII	AAGCTT	1 cut(s)	400
HinfI	GANTC	7 cut(s)	29, 86, 377, 458, 527, 684, 734
HpaII	CCGG	2 cut(s)	52, 66
Hpy166II	GTNNAC	1 cut(s)	172
Hpy188I	TCNGA	1 cut(s)	341
Hpy188III	TCNNGA	6 cut(s)	107, 119, 146, 455, 482, 524
Hpy8I	GTNNAC	1 cut(s)	172
HpyAV	CCTTC	2 cut(s)	647, 674
HpyCH4III	ACNGT	2 cut(s)	333, 614
HpyCH4IV	ACGT	3 cut(s)	24, 139, 549
HpyCH4V	TGCA	4 cut(s)	9, 208, 537, 625
HpyF10VI	GCNNNNNNNGC	3 cut(s)	15, 44, 75
HpyF3I	CTNAG	2 cut(s)	387, 597
HpySE526I	ACGT	3 cut(s)	24, 139, 549
Hsp92II	CATG	2 cut(s)	644, 661
HspAI	GCGC	1 cut(s)	620
Ksp22I	TGATCA	1 cut(s)	747
Kzo9I	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
Lsp1109I	GCAGC	2 cut(s)	220, 609
MaeI	CTAG	1 cut(s)	266
MaeII	ACGT	3 cut(s)	24, 139, 549
MaeIII	GTNAC	2 cut(s)	304, 555
MalI	GATC	6 cut(s)	61, 150, 188, 663, 749, 780
MboI	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
MboII	GAAGA	7 cut(s)	109, 443, 445, 497, 527, 721, 724
MfeI	CAATTG	1 cut(s)	510
MlyI	GAGTC	2 cut(s)	386, 728
MnlI	CCTC	5 cut(s)	89, 242, 696, 699, 763
MroXI	GAANNNNTTC	2 cut(s)	519, 716
MseI	TTAA	3 cut(s)	413, 422, 443
MslI	CAYNNNNRTG	1 cut(s)	179
MspI	CCGG	2 cut(s)	52, 66
MspR9I	CCNGG	2 cut(s)	52, 125
MunI	CAATTG	1 cut(s)	510
MvaI	CCWGG	1 cut(s)	125
MwoI	GCNNNNNNNGC	3 cut(s)	15, 44, 75
NciI	CCSGG	1 cut(s)	52
NcoI	CCATGG	1 cut(s)	657
NdeII	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
NlaIII	CATG	2 cut(s)	644, 661
NmuCI	GTSAC	1 cut(s)	555
NspV	TTCGAA	2 cut(s)	32, 755
PceI	AGGCCT	1 cut(s)	762
PdmI	GAANNNNTTC	2 cut(s)	519, 716
PfeI	GAWTC	5 cut(s)	29, 86, 458, 527, 684
PkrI	GCNGC	3 cut(s)	49, 210, 624
PleI	GAGTC	2 cut(s)	385, 728
PpsI	GAGTC	2 cut(s)	385, 728
Ppu21I	YACGTR	2 cut(s)	140, 550
Psp6I	CCWGG	1 cut(s)	123
PspGI	CCWGG	1 cut(s)	123
PspPI	GGNCC	1 cut(s)	45
PstI	CTGCAG	2 cut(s)	210, 627
PstNI	CAGNNNCTG	1 cut(s)	214
RsaI	GTAC	1 cut(s)	142
RsaNI	GTAC	1 cut(s)	141
RseI	CAYNNNNRTG	1 cut(s)	179
SaqAI	TTAA	3 cut(s)	413, 422, 443
SatI	GCNGC	3 cut(s)	48, 209, 623
Sau3AI	GATC	6 cut(s)	59, 148, 186, 661, 747, 778
Sau96I	GGNCC	1 cut(s)	45
SchI	GAGTC	2 cut(s)	386, 728
ScrFI	CCNGG	2 cut(s)	52, 125
SfcI	CTRYAG	2 cut(s)	206, 623
SfuI	TTCGAA	2 cut(s)	32, 755
SmiMI	CAYNNNNRTG	1 cut(s)	179
SmlI	CTYRAG	1 cut(s)	344
SmoI	CTYRAG	1 cut(s)	344
SnaBI	TACGTA	1 cut(s)	140
SpeI	ACTAGT	1 cut(s)	265
SseBI	AGGCCT	1 cut(s)	762
SsiI	CCGC	2 cut(s)	48, 406
SspI	AATATT	1 cut(s)	631
SspMI	CTAG	1 cut(s)	266
StuI	AGGCCT	1 cut(s)	762
StyD4I	CCNGG	2 cut(s)	50, 123
StyI	CCWWGG	1 cut(s)	657
TaaI	ACNGT	2 cut(s)	333, 614
TaiI	ACGT	3 cut(s)	27, 142, 552
TaqI	TCGA	5 cut(s)	32, 301, 431, 720, 755
TaqII	GACCGA	1 cut(s)	255
TauI	GCSGC	1 cut(s)	50
TfiI	GAWTC	5 cut(s)	29, 86, 458, 527, 684
Tru1I	TTAA	3 cut(s)	413, 422, 443
Tru9I	TTAA	3 cut(s)	413, 422, 443
TseFI	GTSAC	1 cut(s)	555
TseI	GCWGC	2 cut(s)	208, 622
Tsp45I	GTSAC	1 cut(s)	555
TspDTI	ATGAA	4 cut(s)	126, 336, 360, 629
TspGWI	ACGGA	1 cut(s)	71
XagI	CCTNNNNNAGG	1 cut(s)	767
XapI	RAATTY	4 cut(s)	253, 367, 409, 424
XmnI	GAANNNNTTC	2 cut(s)	519, 716
XspI	CTAG	1 cut(s)	266

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.