Rroxscaffold_3G00271080

Plant intracellular Ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005398
Physical Location & Seq
Forward (+)
63502119 .. 63505878
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271080.1

Sequence Viewer

Length: 606 bp
ATGGATATTGGCAAGTTAGTAAACCTGCAACGACTGGTCTTAGCTGAAAACCTCATTGATCGACTACCAATGCATGTGTCAAAATTAGAATCTCTAAAAGTTATGACACTTGATGCAAATCGAATCACTTCCTTGCCTGATGAATTGGGACAGCTGGTGAGGCTTGAGCGGTTATCCATCTCTAAAAATTCTCTATCATATTTGCCTGAGACTATTGGAAGCTTGCGCAATTTAGTGCTGTTAAATGTATCAAACAACAAGTTAAAGTCTCTTCCAGAATCAGTTGGGAGTTGCTTCTCTCTAGAAGAATTACAAGCAGATGATAATTCAATTGAAGATCTTCCTGAATCCGTCTGCAATCTCGTTCACTTAAAGTCACTTTGCTTGGACAATAATAATGTGAAGCAGATACCTACCAATCTATTGAAAGACTGTAAAGTTCTGCAGAATATTTCCTTGCATGGAAATCCTATATCAATGGATCAGTTTCAGCAAATGGAAGGATTCCAAGAGTTTGAAGCAAGGAGGAAGAAGAAGTTCGATAAACAGATTGACTCAAATGTAATGATCAGTTCCAAAGGCCTTGATGAGGGTGTTGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8

Protein Analysis

201

Amino Acids

22.61

Weight (kDa)

5.43

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 82 1.5e-10 Leucine-rich repeat region
LRR_8 PF13855 8 - 65 1.5e-08 Leucine rich repeat
LRR_14 PF23598 25 - 105 2.8e-13 Leucine-rich repeat region
LRR_8 PF13855 31 - 88 2.6e-09 Leucine rich repeat
LRR_14 PF23598 70 - 186 2.2e-10 Leucine-rich repeat region
LRR_8 PF13855 100 - 158 5.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 227
Acc36I ACCTGC 1 cut(s) 33
AccBSI CCGCTC 1 cut(s) 169
AciI CCGC 1 cut(s) 169
AclWI GGATC 1 cut(s) 489
AcsI RAATTY 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 589
AgsI TTSAA 4 cut(s) 330, 335, 427, 518
AluBI AGCT 3 cut(s) 44, 154, 222
AluI AGCT 3 cut(s) 44, 154, 222
Alw26I GTCTC 2 cut(s) 203, 273
AlwI GGATC 1 cut(s) 489
AoxI GGCC 1 cut(s) 580
ApoI RAATTY 1 cut(s) 187
Asp700I GAANNNNTTC 3 cut(s) 127, 339, 536
AspLEI GCGC 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 169
BccI CCATC 1 cut(s) 185
BclI TGATCA 2 cut(s) 567, 598
BcoDI GTCTC 2 cut(s) 203, 273
BfaI CTAG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 443
BfuAI ACCTGC 1 cut(s) 33
BglII AGATCT 1 cut(s) 337
BmsI GCATC 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 117
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse1I ACTGG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 117
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 1 cut(s) 39
BshFI GGCC 1 cut(s) 582
BslFI GGGAC 1 cut(s) 162
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 2 cut(s) 203, 273
BsmFI GGGAC 1 cut(s) 162
BsnI GGCC 1 cut(s) 582
Bsp143I GATC 5 cut(s) 58, 337, 481, 567, 598
BspACI CCGC 1 cut(s) 169
BspANI GGCC 1 cut(s) 582
BspCNI CTCAG 1 cut(s) 199
BspMAI CTGCAG 1 cut(s) 447
BspMI ACCTGC 1 cut(s) 33
BspPI GGATC 1 cut(s) 489
BsrBI CCGCTC 1 cut(s) 169
BsrI ACTGG 1 cut(s) 39
BssMI GATC 5 cut(s) 58, 337, 481, 567, 598
Bst4CI ACNGT 1 cut(s) 434
Bst6I CTCTTC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 224
BstDEI CTNAG 2 cut(s) 40, 207
BstENI CCTNNNNNAGG 1 cut(s) 587
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 5 cut(s) 61, 340, 484, 570, 601
BstMAI GTCTC 2 cut(s) 203, 273
BstMBI GATC 5 cut(s) 58, 337, 481, 567, 598
BstMWI GCNNNNNNNGC 1 cut(s) 160
BstNSI RCATGY 1 cut(s) 77
BstSFI CTRYAG 1 cut(s) 443
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 582
BveI ACCTGC 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 224
CfoI GCGC 1 cut(s) 228
CviAII CATG 2 cut(s) 74, 461
CviJI RGCY 5 cut(s) 44, 154, 163, 222, 582
CviKI_1 RGCY 5 cut(s) 44, 154, 163, 222, 582
DdeI CTNAG 2 cut(s) 40, 207
DpnI GATC 5 cut(s) 60, 339, 483, 569, 600
DpnII GATC 5 cut(s) 58, 337, 481, 567, 598
Eam1104I CTCTTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 276
Eco147I AGGCCT 1 cut(s) 582
EcoNI CCTNNNNNAGG 1 cut(s) 587
EcoT22I ATGCAT 1 cut(s) 75
FaeI CATG 2 cut(s) 77, 464
FaiI YATR 5 cut(s) 75, 104, 199, 462, 473
FaqI GGGAC 1 cut(s) 162
FatI CATG 2 cut(s) 73, 460
FbaI TGATCA 2 cut(s) 567, 598
FspBI CTAG 1 cut(s) 302
FspI TGCGCA 1 cut(s) 227
GlaI GCGC 1 cut(s) 227
HaeIII GGCC 1 cut(s) 582
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 77, 464
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 220
HinfI GANTC 6 cut(s) 89, 123, 278, 347, 504, 554
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 22, 367
Hpy188III TCNNGA 3 cut(s) 275, 302, 344
Hpy8I GTNNAC 2 cut(s) 22, 367
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 1 cut(s) 434
HpyCH4V TGCA 6 cut(s) 28, 73, 116, 357, 445, 460
HpyF10VI GCNNNNNNNGC 1 cut(s) 160
HpyF3I CTNAG 2 cut(s) 40, 207
Hsp92II CATG 2 cut(s) 77, 464
HspAI GCGC 1 cut(s) 226
Ksp22I TGATCA 2 cut(s) 567, 598
Kzo9I GATC 5 cut(s) 58, 337, 481, 567, 598
LpnPI CCDG 7 cut(s) 20, 38, 140, 150, 219, 288, 357
LweI GCATC 1 cut(s) 103
MaeI CTAG 1 cut(s) 302
MaeIII GTNAC 1 cut(s) 375
MalI GATC 5 cut(s) 60, 339, 483, 569, 600
MbiI CCGCTC 1 cut(s) 169
MboI GATC 5 cut(s) 58, 337, 481, 567, 598
MboII GAAGA 6 cut(s) 263, 317, 332, 347, 541, 544
MfeI CAATTG 1 cut(s) 330
MflI RGATCY 1 cut(s) 337
MluCI AATT 7 cut(s) 83, 143, 187, 229, 308, 325, 330
MlyI GAGTC 1 cut(s) 548
MnlI CCTC 4 cut(s) 62, 153, 519, 583
Mph1103I ATGCAT 1 cut(s) 75
MroXI GAANNNNTTC 3 cut(s) 127, 339, 536
MseI TTAA 3 cut(s) 242, 263, 371
MspA1I CMGCKG 1 cut(s) 154
MunI CAATTG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 160
NdeII GATC 5 cut(s) 58, 337, 481, 567, 598
NlaIII CATG 2 cut(s) 77, 464
NmuCI GTSAC 1 cut(s) 375
NsbI TGCGCA 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 75
NspI RCATGY 1 cut(s) 77
PceI AGGCCT 1 cut(s) 582
PdmI GAANNNNTTC 3 cut(s) 127, 339, 536
PfeI GAWTC 5 cut(s) 89, 123, 278, 347, 504
PleI GAGTC 1 cut(s) 548
PpsI GAGTC 1 cut(s) 548
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 154
SaqAI TTAA 3 cut(s) 242, 263, 371
Sau3AI GATC 5 cut(s) 58, 337, 481, 567, 598
SchI GAGTC 1 cut(s) 548
SetI ASST 6 cut(s) 27, 46, 54, 156, 224, 415
SfaNI GCATC 1 cut(s) 103
SfcI CTRYAG 1 cut(s) 443
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 7 cut(s) 83, 143, 187, 229, 308, 325, 330
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 1 cut(s) 169
SspI AATATT 1 cut(s) 451
SspMI CTAG 1 cut(s) 302
StuI AGGCCT 1 cut(s) 582
TaaI ACNGT 1 cut(s) 434
TaqI TCGA 3 cut(s) 61, 121, 540
TasI AATT 7 cut(s) 83, 143, 187, 229, 308, 325, 330
TfiI GAWTC 5 cut(s) 89, 123, 278, 347, 504
Tru1I TTAA 3 cut(s) 242, 263, 371
Tru9I TTAA 3 cut(s) 242, 263, 371
TseFI GTSAC 1 cut(s) 375
Tsp45I GTSAC 1 cut(s) 375
TspDTI ATGAA 1 cut(s) 156
TspGWI ACGGA 1 cut(s) 340
XagI CCTNNNNNAGG 1 cut(s) 587
XapI RAATTY 1 cut(s) 187
XbaI TCTAGA 1 cut(s) 301
XceI RCATGY 1 cut(s) 77
XmnI GAANNNNTTC 3 cut(s) 127, 339, 536
XspI CTAG 1 cut(s) 302
Zsp2I ATGCAT 1 cut(s) 75
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.