Rh7AG052800

Plant intracellular Ras-group-related LRR protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0005398
Physical Location & Seq
Reverse (-)
3658608 .. 3662219
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG052800.1

Sequence Viewer

Length: 567 bp
ATGTGTTGTTGTTTATATTGTCTCTGCACATATTCTTTGTTCTTTACTTACCGGTTACTTTTTCTGGAGCAGACATTTCCAGATGAAGTTCTTGATCTGGAGAGATCCGTACGCACTATTGATCTAACGCGCAATAAAATTGTTGATATTCCTATGGATATTGGCAAGTTAGTAAACCTGCAACGACTGGTCTTAGCTGAAAACCTCATTGATCGACTACCAATGCATGTGTCAAAATTAGAATCTCTAAAAGTTATGACACTTGATGCAAATCGAATCACTTCCTTGCCTGATGAATTGGGACAGCTGGTGAGGCTTGAGCGGTTATCCATCTCTAAAAATTCTCTATCATATTTGCCTGAGACTATTGGAAGCTTGCGCAATTTAGTGCTGTTAAATGTATCAAACAACAAGTTAAAGTCTCTTCCAGAATCAGTTGGGAGTTGCTTCTCTCTAGAAGAATTACAAGCAGATGATAATTCAATTGAAGATCTTCCCGAATCCGTCTGCAATCTTGTTCACTTAAAGTCACTTTGCTTGGACAATAATAATGTGAAGCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
LRR_8

Protein Analysis

188

Amino Acids

21.35

Weight (kDa)

4.99

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 35 - 88 1.3e-06 Leucine rich repeat
LRR_14 PF23598 37 - 110 1.2e-10 Leucine-rich repeat region
LRR_14 PF23598 76 - 157 1.2e-13 Leucine-rich repeat region
LRR_8 PF13855 82 - 139 1.9e-09 Leucine rich repeat
LRR_14 PF23598 132 - 183 6.7e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 380
Acc36I ACCTGC 2 cut(s) 186, 550
AccBSI CCGCTC 1 cut(s) 322
AccII CGCG 1 cut(s) 130
AciI CCGC 1 cut(s) 322
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 340
AfaI GTAC 1 cut(s) 111
AgeI ACCGGT 1 cut(s) 51
AgsI TTSAA 2 cut(s) 483, 488
AluBI AGCT 3 cut(s) 197, 307, 375
AluI AGCT 3 cut(s) 197, 307, 375
Alw26I GTCTC 3 cut(s) 26, 356, 426
AlwI GGATC 1 cut(s) 99
ApoI RAATTY 1 cut(s) 340
AsiGI ACCGGT 1 cut(s) 51
Asp700I GAANNNNTTC 2 cut(s) 280, 492
AspLEI GCGC 2 cut(s) 132, 381
AsuHPI GGTGA 1 cut(s) 322
BccI CCATC 1 cut(s) 338
BcoDI GTCTC 3 cut(s) 26, 356, 426
BfaI CTAG 1 cut(s) 455
BfuAI ACCTGC 2 cut(s) 186, 550
BglII AGATCT 1 cut(s) 490
BmsI GCATC 1 cut(s) 256
BpmI CTGGAG 2 cut(s) 86, 119
BpuEI CTTGAG 1 cut(s) 338
BsaBI GATNNNNATC 1 cut(s) 270
BsaWI WCCGGW 1 cut(s) 51
Bse118I RCCGGY 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 270
BseJI GATNNNNATC 1 cut(s) 270
BseMII CTCAG 1 cut(s) 351
BseNI ACTGG 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 130
BshTI ACCGGT 1 cut(s) 51
BsiSI CCGG 1 cut(s) 52
BsiWI CGTACG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 315
BsmAI GTCTC 3 cut(s) 26, 356, 426
BsmFI GGGAC 1 cut(s) 315
Bsp143I GATC 5 cut(s) 94, 104, 121, 211, 490
BspACI CCGC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 352
BspFNI CGCG 1 cut(s) 130
BspMI ACCTGC 2 cut(s) 186, 550
BspPI GGATC 1 cut(s) 99
BsrBI CCGCTC 1 cut(s) 322
BsrFI RCCGGY 1 cut(s) 51
BsrI ACTGG 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 51
BssMI GATC 5 cut(s) 94, 104, 121, 211, 490
Bst6I CTCTTC 1 cut(s) 429
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 2 cut(s) 193, 360
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 2 cut(s) 132, 381
BstKTI GATC 5 cut(s) 97, 107, 124, 214, 493
BstMAI GTCTC 3 cut(s) 26, 356, 426
BstMBI GATC 5 cut(s) 94, 104, 121, 211, 490
BstMWI GCNNNNNNNGC 1 cut(s) 313
BstNSI RCATGY 1 cut(s) 230
BstUI CGCG 1 cut(s) 130
BstX2I RGATCY 2 cut(s) 104, 490
BstYI RGATCY 2 cut(s) 104, 490
BveI ACCTGC 2 cut(s) 186, 550
Cac8I GCNNGC 1 cut(s) 377
CfoI GCGC 2 cut(s) 132, 381
Cfr10I RCCGGY 1 cut(s) 51
Csp6I GTAC 1 cut(s) 110
CspAI ACCGGT 1 cut(s) 51
CviAII CATG 1 cut(s) 227
CviJI RGCY 4 cut(s) 197, 307, 316, 375
CviKI_1 RGCY 4 cut(s) 197, 307, 316, 375
CviQI GTAC 1 cut(s) 110
DdeI CTNAG 2 cut(s) 193, 360
DpnI GATC 5 cut(s) 96, 106, 123, 213, 492
DpnII GATC 5 cut(s) 94, 104, 121, 211, 490
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
EcoT22I ATGCAT 1 cut(s) 228
FaeI CATG 1 cut(s) 230
FaiI YATR 7 cut(s) 16, 31, 155, 228, 257, 352, 565
FaqI GGGAC 1 cut(s) 315
FatI CATG 1 cut(s) 226
FspBI CTAG 1 cut(s) 455
FspI TGCGCA 1 cut(s) 380
GlaI GCGC 2 cut(s) 131, 380
GsuI CTGGAG 2 cut(s) 86, 119
HapII CCGG 1 cut(s) 52
HhaI GCGC 2 cut(s) 132, 381
Hin1II CATG 1 cut(s) 230
Hin6I GCGC 2 cut(s) 130, 379
HinP1I GCGC 2 cut(s) 130, 379
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 4 cut(s) 242, 276, 431, 500
HpaII CCGG 1 cut(s) 52
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 2 cut(s) 175, 520
Hpy188III TCNNGA 7 cut(s) 65, 80, 92, 98, 428, 455, 497
Hpy8I GTNNAC 2 cut(s) 175, 520
HpyCH4V TGCA 5 cut(s) 27, 181, 226, 269, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 313
HpyF3I CTNAG 2 cut(s) 193, 360
Hsp92II CATG 1 cut(s) 230
HspAI GCGC 2 cut(s) 130, 379
Kzo9I GATC 5 cut(s) 94, 104, 121, 211, 490
LmnI GCTCC 1 cut(s) 67
LweI GCATC 1 cut(s) 256
MaeI CTAG 1 cut(s) 455
MaeIII GTNAC 2 cut(s) 54, 528
MalI GATC 5 cut(s) 96, 106, 123, 213, 492
MbiI CCGCTC 1 cut(s) 322
MboI GATC 5 cut(s) 94, 104, 121, 211, 490
MboII GAAGA 4 cut(s) 416, 470, 485, 500
MfeI CAATTG 1 cut(s) 483
MflI RGATCY 2 cut(s) 104, 490
MluCI AATT 8 cut(s) 138, 236, 296, 340, 382, 461, 478, 483
MnlI CCTC 2 cut(s) 215, 306
Mph1103I ATGCAT 1 cut(s) 228
MroXI GAANNNNTTC 2 cut(s) 280, 492
MseI TTAA 3 cut(s) 395, 416, 524
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 483
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 1 cut(s) 313
NdeII GATC 5 cut(s) 94, 104, 121, 211, 490
NlaIII CATG 1 cut(s) 230
NmuCI GTSAC 1 cut(s) 528
NsbI TGCGCA 1 cut(s) 380
NsiI ATGCAT 1 cut(s) 228
NspI RCATGY 1 cut(s) 230
PdmI GAANNNNTTC 2 cut(s) 280, 492
PfeI GAWTC 4 cut(s) 242, 276, 431, 500
Pfl23II CGTACG 1 cut(s) 109
PinAI ACCGGT 1 cut(s) 51
PspLI CGTACG 1 cut(s) 109
PsuI RGATCY 2 cut(s) 104, 490
PvuII CAGCTG 1 cut(s) 307
RsaI GTAC 1 cut(s) 111
RsaNI GTAC 1 cut(s) 110
SaqAI TTAA 3 cut(s) 395, 416, 524
Sau3AI GATC 5 cut(s) 94, 104, 121, 211, 490
SetI ASST 6 cut(s) 180, 199, 207, 309, 377, 564
SfaNI GCATC 1 cut(s) 256
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 8 cut(s) 138, 236, 296, 340, 382, 461, 478, 483
SsiI CCGC 1 cut(s) 322
SspMI CTAG 1 cut(s) 455
TaqI TCGA 2 cut(s) 214, 274
TasI AATT 8 cut(s) 138, 236, 296, 340, 382, 461, 478, 483
TfiI GAWTC 4 cut(s) 242, 276, 431, 500
Tru1I TTAA 3 cut(s) 395, 416, 524
Tru9I TTAA 3 cut(s) 395, 416, 524
TseFI GTSAC 1 cut(s) 528
Tsp45I GTSAC 1 cut(s) 528
TspDTI ATGAA 2 cut(s) 99, 309
TspGWI ACGGA 2 cut(s) 97, 493
XapI RAATTY 1 cut(s) 340
XbaI TCTAGA 1 cut(s) 454
XceI RCATGY 1 cut(s) 230
XmnI GAANNNNTTC 2 cut(s) 280, 492
XspI CTAG 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.