Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012025

Physical Location & Seq

Genomic Coordinates Reverse (-)

30201633 .. 30209596

Gene Structure 7964 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1219700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2973 bp

ATGGCTTCAGACGAAGACGATTTCGTGTTCTACGGAACACCGATTGAGCGGGAGGAAGATTTCACCAGTCGCAAGAAGAAGTCCGTTGCCGAGGCCTCCGGTAACTTGCGTACTCTCGTTCCTTGGAAGCAAGAGGTTAGAGATGAAGAAGGGAGAAGGAGATTTCATGGTGCATTTAGTGGAGGGTTTTCAGCTGGCTATTACAATACAGTCGGCTCAAAAGAAGGGTGGACACCGCAGACCTTTGTGTCATCGCGGAAGAACAGAGCTGAAGTCAAACGGCAGGACATTATGAACTTTTTAGATGAAGATGAAAGAGCTGAACTTGAAGGCCAATCTTTGGGGACGTCTATGCAGTTTGATACATTTGGATCTACAGCTGCTGAACTTGCTCGTAAACAAGCTGAGAAGGAACAACAGAAAAGGCCATCAGCTATTCCCGGACCAGTTCCTGATGAATTAGTTCTTCCAGCCACAGATTCTATAGGTGTGAAGTTGCTGCTGAAGATGGGATGGCGCCATGGTCGTTCAATCAAGGATTCACATACCGATTCATCATACGATGCTCGAAGGGAAGCGCGAAAAGCATTTTTAGCATTTTCTTCCAGTGATGCAAAAGCACAGCTTGCTGACTCTGAGCCTGTTCATGGCGAAATGGAGGATCATATTGAGCTGCCTGCTAGTGATGATGTTCAATCTTCTCAAAGCACACCTGTTTATGTTCTCTACCCAAAGCAGGACCTTCACGGATTAGGTTTTGATCCTTATAAGCATGCTCCTGAGTTCAGAGAGAAAAAGAGATCACGCCTCTCTGAGAATAGGGGTATTGGATATAGAAGTGCCCGTTCAATGGACAATAATCTTTTTGGCTTCAAGTCAGGAAAGGTTGCTCCTGGTTTTGGAATTGGAGCACTTGAAGAACTTGATGCTGAAGATGAGGATGTTTATAATTCTGGTTATGGCTTTGAGGAAACTTATGTTGAAGATATTGATGATGAGCCTTCAAGATCAATCATGGATAGCAAGCAAAAGTCCGTTCGAAAGGAACCAGGTGGTCTCTCTGGATTTAGACTTGCATCTAATTCGGACTACCAACATGAGAGATTTGATCCTCCTGTAGTACCAAAAGATTTTGTACCCAACCATAAATTTTCTGGGCCTCTTGATAGTGGCTGCAAGCTTGGAGATCCTGGTCCCACAGAAGTCTCTCCTCCAGAGGATAATAATCTGAAACTCTTAATTGACGGGGTCGCAACTTTAGTAGCTCGATGTGGTAAATTATTTGAGGATCTCTCTAGAGAGAAAAATCAGTCAAATCCCTTGTTTAGTTTTCTAGTTGGAGGGAATGGCCATGATTATTATGCAAGGAAACTGTGGGAGGAGCGGCAGAAGCGAGGCAATAACACCAAGGAGATATTGGATGGTAAATTGTCTCCACGAAAGCAGAAGATGACAGCAGAGAGCCGTGGAAAATTTTTAGGGGAAAGGCCTTTGGAACGAAGCTCCAAAGAGTCAAGTTCATCTGTTGCTTCTGAAGAGGCCATTCAGCTTCAGTACAACCTTTCAGATACATTTACTAAACCTGCATTAGATGGTGAGATGCTGGAAGTTGCAAAACCCTTCAACGATGATCCTGCAAAGCAAGAAAGGTTTGAGCGGTTTCTCAAGGAGAAATACCAAGGAGGACTTCGATCTACTGAGTCTGGTGGAGCTAGTCATATGTCAGAAGCAGTTCGTGCTCGTGAGAGATTAGACTTTGAGGCTGCAGCTGATGCAATACAGAAAGGGAAGTGGAGTAAAGAAAGCAAACTCTCCACATCACAGTTCATGGATTTTTTATCTGCTGGAGCCATGCAGTTTACTTCTGGGGGATCAGCGCAAGCCAAAGACACTCAAACTGAAAATTCTATAAAAAAGGAAGTTCGCATAAAACGCACAGAATATCAGTGGCGTCCCTCACCTATTTTATGCAAACGTTTTGATCTCATCGATCCTTTCATGGGGAAGCCACCACCAGCACCCCGGATGAAGAGCAAAATGGAAACTCTGATTTTTACATCAGATTCTGGAAAAGACACAAAAGCAGAAGAAACTGTAATTGTAAAGAGTGACTACATCCCTGTTGTTCAATCTAATGCCCAAGGAATAAGCAAAGATGTAGTTGACGAGGAAAGCGCAGTTGAGGTTGAAGTTGACAACGTCGAGAGGCCAGTTGACCTTTACAAGGCTATATTTTCTGATGACGAGGACGATGATGAGGATACCTCTGTTTCCAACGGGGTGGGTAATCCAGAGAAGAAGGTTGAAGCAGCCAATACAACATTAAACCGTTTGATCGCAGGCGACTTTTTGGAATCCTTAGGAAAAGAACTAGGCCTAGAGGTTCCCCCTGAGCTGCCCTCATCAACGAACAAAGCCAGGACTTCTTCGCCTCCCAAAGGAGCTGGTTCAGGATCTTCCAGTATCCTGCCAGTTGATAATAAGCCGCCTTCCACCCATGAGATTCCGCGCAGACCAGAAATTTCTCAGGAGGCTCCGCGTGACAATGCTGAACCTTTTAATGGTAACTTGACGGGTAATTCAGCGAGAAGCAATGGCAAAAACGCAGAGATGGGTGCTTTGGGAAATCAGTTTGACAAGATTATTTTTCGAAAAGCACATCAAGATGATAGGAAGGACAAAACACCCTCAAGCCGGCACCACAATGTGAGCAGCAGTTCACCATCAGAAGATGAAAGGAGCAGAAGGCGCTCTAGGGGACATCGGCATAGACACGGTGATTCAGATAGCGATTCATCCAGCGATCATCAGGACCGTCACCGTTCTAGGTCCAAAGTTAAGAAGAAAGGATCCTCTCGAGAAAAGAGCAGTAGTAGCAGAAAACGCTCGAAGCATCATAAACGTCGAAGCAGAGACTCCCCTGAGAAGGAACGTTCAGAATCCAGGAGAGACAAGCACAGAAGGCGAGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000375 GO:0000377 GO:0000398 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0008380 GO:0009888 GO:0009892 GO:0009987 GO:0010033 GO:0010087 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016071 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0032502 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048519 GO:0048856 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070878 GO:0070883 GO:0070887 GO:0070918 GO:0071013 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1604 Surp

Protein Analysis

991

Amino Acids

110.64

Weight (kDa)

6.51

Isoelectric Point (pI)

61.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF1604	PF07713	41 - 118	4.7e-35	Protein of unknown function (DUF1604)
Surp	PF01805	413 - 460	3.5e-11	Surp module
HTH_TGH	PF26093	519 - 595	6.1e-22	TGH-like, helix-turn-helix domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012025)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G23080 AT5G23080
fragaria_vesca	FvH4_5g12911 FvH4_5g12911
malus_domestica	MD06G1209200.v1.1 MD14G1219700.v1.1
prunus_persica	Prupe.5G214300_v2.0.a1
pyrus_communis	pycom06g18680 pycom14g18200
rosa_chinensis	RchiOBHm_Chr7g0184311
rosa_laevigata	RLG00000004987
rosa_multiflora	Rmu_co8385771.1_g000001 Rmu_co8404991.1_g000001
rosa_roxburghii	Rroxscaffold_3G00269740
rosa_samantha	Rh7AG081200 Rh7BG066100 Rh7CG066400 Rh7DG066100
rosa_wichuraiana	Rw7G005420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	768, 948
AasI	GACNNNNNNGTC	1 cut(s)	247
AatII	GACGTC	1 cut(s)	350
Acc36I	ACCTGC	1 cut(s)	1591
AccB1I	GGYRCC	2 cut(s)	516, 2697
AccB7I	CCANNNNNTGG	1 cut(s)	340
AccBSI	CCGCTC	3 cut(s)	49, 1384, 1657
AccII	CGCG	4 cut(s)	256, 580, 2509, 2539
AciI	CCGC	8 cut(s)	49, 236, 256, 1384, 1657, 2486, 2507, 2537
AclI	AACGTT	2 cut(s)	1975, 2932
AcoI	YGGCCR	1 cut(s)	1348
AcsI	RAATTY	4 cut(s)	1148, 1472, 1903, 2520
AcuI	CTGAAG	5 cut(s)	291, 524, 951, 1535, 1554
AcyI	GRCGYC	3 cut(s)	347, 517, 1951
AdeI	CACNNNGTG	1 cut(s)	2707
AfaI	GTAC	4 cut(s)	112, 1122, 1137, 1556
AjnI	CCWGG	5 cut(s)	892, 1048, 1189, 2417, 2942
AjuI	GAANNNNNNNTTGG	2 cut(s)	1671, 1703
Alw21I	GWGCWC	2 cut(s)	913, 1741
Alw26I	GTCTC	5 cut(s)	1061, 1210, 1437, 2907, 2943
AlwNI	CAGNNNCTG	3 cut(s)	383, 452, 2066
Ama87I	CYCGRG	1 cut(s)	2856
AoxI	GGCC	9 cut(s)	93, 331, 425, 1157, 1348, 1487, 1539, 2207, 2374
ApeKI	GCWGC	9 cut(s)	380, 499, 673, 1173, 1763, 1766, 2309, 2395, 2712
ApoI	RAATTY	4 cut(s)	1148, 1472, 1903, 2520
Asp700I	GAANNNNTTC	2 cut(s)	462, 1731
AspLEI	GCGC	6 cut(s)	519, 580, 1879, 2177, 2511, 2751
AspS9I	GGNCC	6 cut(s)	443, 739, 1157, 1193, 2812, 2829
AsuC2I	CCSGG	2 cut(s)	441, 2023
AsuHPI	GGTGA	6 cut(s)	55, 1607, 1950, 2712, 2789, 2810
AsuII	TTCGAA	2 cut(s)	1039, 2650
AvaI	CYCGRG	1 cut(s)	2856
AvaII	GGWCC	5 cut(s)	443, 739, 1193, 2812, 2829
AxyI	CCTNAGG	1 cut(s)	2359
BaeGI	GKGCMC	1 cut(s)	844
BaeI	ACNNNNGTAYC	2 cut(s)	2253, 2286
BalI	TGGCCA	1 cut(s)	1350
BamHI	GGATCC	1 cut(s)	2849
BanI	GGYRCC	2 cut(s)	516, 2697
BauI	CACGAG	1 cut(s)	1740
BbsI	GAAGAC	1 cut(s)	21
Bbv12I	GWGCWC	2 cut(s)	913, 1741
BbvI	GCAGC	9 cut(s)	367, 486, 660, 1160, 1750, 1778, 2321, 2382, 2724
BccI	CCATC	7 cut(s)	436, 502, 507, 1415, 1586, 2605, 2731
BceAI	ACGGC	2 cut(s)	296, 1449
BcgI	CGANNNNNNTGC	4 cut(s)	1065, 1099, 1616, 1650
BciT130I	CCWGG	5 cut(s)	894, 1050, 1191, 2419, 2944
BciVI	GTATCC	2 cut(s)	2254, 2474
BcnI	CCSGG	2 cut(s)	441, 2023
BcoDI	GTCTC	5 cut(s)	1061, 1210, 1437, 2907, 2943
BfaI	CTAG	8 cut(s)	681, 1296, 1334, 1713, 2372, 2378, 2754, 2826
BfmI	CTRYAG	4 cut(s)	375, 483, 1116, 1764
BfoI	RGCGCY	2 cut(s)	520, 2752
BfuAI	ACCTGC	1 cut(s)	1591
BfuI	GTATCC	2 cut(s)	2254, 2474
Bme1390I	CCNGG	7 cut(s)	441, 894, 1050, 1191, 2023, 2419, 2944
Bme18I	GGWCC	5 cut(s)	443, 739, 1193, 2812, 2829
BmeT110I	CYCGRG	1 cut(s)	2856
BmgT120I	GGNCC	6 cut(s)	443, 739, 1157, 1193, 2812, 2829
BmiI	GGNNCC	8 cut(s)	518, 1047, 1195, 1849, 2385, 2535, 2699, 2851
BmrFI	CCNGG	7 cut(s)	441, 894, 1050, 1191, 2023, 2419, 2944
BmsI	GCATC	7 cut(s)	553, 601, 916, 1085, 1590, 1762, 2902
BpiI	GAAGAC	1 cut(s)	21
BplI	GAGNNNNNCTC	6 cut(s)	1277, 1309, 2249, 2281, 2384, 2416
BpmI	CTGGAG	2 cut(s)	1197, 1866
Bpu10I	CCTNAGC	1 cut(s)	2391
Bpu14I	TTCGAA	2 cut(s)	1039, 2650
BpuEI	CTTGAG	2 cut(s)	1649, 2674
BpuMI	CCSGG	2 cut(s)	441, 2023
Bsa29I	ATCGAT	1 cut(s)	1989
BsaBI	GATNNNNATC	1 cut(s)	1224
BsaHI	GRCGYC	3 cut(s)	347, 517, 1951
BsaI	GGTCTC	1 cut(s)	1061
BsaJI	CCNNGG	8 cut(s)	90, 122, 520, 1407, 1465, 1678, 2021, 2140
BsaWI	WCCGGW	1 cut(s)	98
Bse118I	RCCGGY	1 cut(s)	2694
Bse1I	ACTGG	6 cut(s)	66, 446, 606, 2210, 2460, 2471
Bse21I	CCTNAGG	1 cut(s)	2359
Bse3DI	GCAATG	1 cut(s)	2599
Bse8I	GATNNNNATC	1 cut(s)	1224
BseBI	CCWGG	5 cut(s)	894, 1050, 1191, 2419, 2944
BseCI	ATCGAT	1 cut(s)	1989
BseDI	CCNNGG	8 cut(s)	90, 122, 520, 1407, 1465, 1678, 2021, 2140
BseGI	GGATG	6 cut(s)	518, 946, 1426, 2031, 2115, 2795
BseJI	GATNNNNATC	1 cut(s)	1224
BseMI	GCAATG	1 cut(s)	2599
BseMII	CTCAG	8 cut(s)	396, 627, 771, 804, 1689, 2382, 2540, 2913
BseNI	ACTGG	6 cut(s)	66, 446, 606, 2210, 2460, 2471
BseRI	GAGGAG	2 cut(s)	1200, 1394
BseSI	GKGCMC	1 cut(s)	844
BseXI	GCAGC	9 cut(s)	367, 486, 660, 1160, 1750, 1778, 2321, 2382, 2724
Bsh1236I	CGCG	4 cut(s)	256, 580, 2509, 2539
BshFI	GGCC	9 cut(s)	95, 333, 427, 1159, 1350, 1489, 1541, 2209, 2376
BshNI	GGYRCC	2 cut(s)	516, 2697
BshVI	ATCGAT	1 cut(s)	1989
BsiHKAI	GWGCWC	2 cut(s)	913, 1741
BsiHKCI	CYCGRG	1 cut(s)	2856
BsiSI	CCGG	4 cut(s)	99, 441, 2023, 2695
BslFI	GGGAC	4 cut(s)	358, 1179, 1938, 2772
BsmAI	GTCTC	5 cut(s)	1061, 1210, 1437, 2907, 2943
BsmFI	GGGAC	4 cut(s)	358, 1179, 1938, 2772
BsnI	GGCC	9 cut(s)	95, 333, 427, 1159, 1350, 1489, 1541, 2209, 2376
Bso31I	GGTCTC	1 cut(s)	1061
BsoBI	CYCGRG	1 cut(s)	2856
Bsp119I	TTCGAA	2 cut(s)	1039, 2650
Bsp1286I	GDGCHC	3 cut(s)	844, 913, 1741
Bsp19I	CCATGG	1 cut(s)	520
BspACI	CCGC	8 cut(s)	49, 236, 256, 1384, 1657, 2486, 2507, 2537
BspANI	GGCC	9 cut(s)	95, 333, 427, 1159, 1350, 1489, 1541, 2209, 2376
BspCNI	CTCAG	8 cut(s)	397, 628, 772, 805, 1690, 2383, 2539, 2914
BspDI	ATCGAT	1 cut(s)	1989
BspFNI	CGCG	4 cut(s)	256, 580, 2509, 2539
BspLI	GGNNCC	8 cut(s)	518, 1047, 1195, 1849, 2385, 2535, 2699, 2851
BspMAI	CTGCAG	1 cut(s)	1768
BspMI	ACCTGC	1 cut(s)	1591
BspQI	GCTCTTC	1 cut(s)	2024
BspT104I	TTCGAA	2 cut(s)	1039, 2650
BspT107I	GGYRCC	2 cut(s)	516, 2697
BspTNI	GGTCTC	1 cut(s)	1061
BsrBI	CCGCTC	3 cut(s)	49, 1384, 1657
BsrDI	GCAATG	1 cut(s)	2599
BsrFI	RCCGGY	1 cut(s)	2694
BsrI	ACTGG	6 cut(s)	66, 446, 606, 2210, 2460, 2471
BssAI	RCCGGY	1 cut(s)	2694
BssECI	CCNNGG	8 cut(s)	90, 122, 520, 1407, 1465, 1678, 2021, 2140
BssNI	GRCGYC	3 cut(s)	347, 517, 1951
BssSI	CACGAG	1 cut(s)	1740
BssT1I	CCWWGG	5 cut(s)	122, 520, 1407, 1678, 2140
Bst2BI	CACGAG	1 cut(s)	1740
Bst2UI	CCWGG	5 cut(s)	894, 1050, 1191, 2419, 2944
Bst4CI	ACNGT	8 cut(s)	211, 1374, 1824, 2095, 2330, 2777, 2816, 2822
Bst6I	CTCTTC	2 cut(s)	1530, 2024
BstACI	GRCGYC	3 cut(s)	347, 517, 1951
BstAPI	GCANNNNNTGC	3 cut(s)	626, 1736, 1772
BstBI	TTCGAA	2 cut(s)	1039, 2650
BstC8I	GCNNGC	9 cut(s)	196, 627, 678, 774, 1025, 1178, 1881, 2341, 2696
BstDEI	CTNAG	9 cut(s)	405, 636, 780, 813, 1698, 2359, 2391, 2526, 2922
BstDSI	CCRYGG	2 cut(s)	520, 1465
BstENI	CCTNNNNNAGG	2 cut(s)	2222, 2436
BstF5I	GGATG	6 cut(s)	518, 946, 1426, 2031, 2115, 2795
BstFNI	CGCG	4 cut(s)	256, 580, 2509, 2539
BstH2I	RGCGCY	2 cut(s)	520, 2752
BstHHI	GCGC	6 cut(s)	519, 580, 1879, 2177, 2511, 2751
BstMAI	GTCTC	5 cut(s)	1061, 1210, 1437, 2907, 2943
BstNI	CCWGG	5 cut(s)	894, 1050, 1191, 2419, 2944
BstNSI	RCATGY	1 cut(s)	776
BstSCI	CCNGG	7 cut(s)	439, 892, 1048, 1189, 2021, 2417, 2942
BstSFI	CTRYAG	4 cut(s)	375, 483, 1116, 1764
BstSLI	GKGCMC	1 cut(s)	844
BstUI	CGCG	4 cut(s)	256, 580, 2509, 2539
BstV1I	GCAGC	9 cut(s)	367, 486, 660, 1160, 1750, 1778, 2321, 2382, 2724
BstV2I	GAAGAC	1 cut(s)	21
BstX2I	RGATCY	5 cut(s)	371, 1186, 1288, 2453, 2849
BstXI	CCANNNNNNTGG	1 cut(s)	2281
BstYI	RGATCY	5 cut(s)	371, 1186, 1288, 2453, 2849
Bsu15I	ATCGAT	1 cut(s)	1989
Bsu36I	CCTNAGG	1 cut(s)	2359
BsuI	GTATCC	2 cut(s)	2254, 2474
BsuRI	GGCC	9 cut(s)	95, 333, 427, 1159, 1350, 1489, 1541, 2209, 2376
BsuTUI	ATCGAT	1 cut(s)	1989
BtgI	CCRYGG	2 cut(s)	520, 1465
BtgZI	GCGATG	1 cut(s)	237
BtsCI	GGATG	6 cut(s)	518, 946, 1426, 2031, 2115, 2795
BtsIMutI	CAGTG	2 cut(s)	613, 1952
BveI	ACCTGC	1 cut(s)	1591
Cac8I	GCNNGC	9 cut(s)	196, 627, 678, 774, 1025, 1178, 1881, 2341, 2696
CaiI	CAGNNNCTG	3 cut(s)	383, 452, 2066
CfoI	GCGC	6 cut(s)	519, 580, 1879, 2177, 2511, 2751
Cfr10I	RCCGGY	1 cut(s)	2694
Cfr13I	GGNCC	6 cut(s)	443, 739, 1157, 1193, 2812, 2829
ClaI	ATCGAT	1 cut(s)	1989
CseI	GACGC	1 cut(s)	1940
CsiI	ACCWGGT	1 cut(s)	1048
Csp6I	GTAC	4 cut(s)	111, 1121, 1136, 1555
CviQI	GTAC	4 cut(s)	111, 1121, 1136, 1555
DdeI	CTNAG	9 cut(s)	405, 636, 780, 813, 1698, 2359, 2391, 2526, 2922
DinI	GGCGCC	1 cut(s)	518
DraIII	CACNNNGTG	1 cut(s)	2707
DrdI	GACNNNNNNGTC	1 cut(s)	247
DseDI	GACNNNNNNGTC	1 cut(s)	247
EaeI	YGGCCR	1 cut(s)	1348
Eam1104I	CTCTTC	2 cut(s)	1530, 2024
EarI	CTCTTC	2 cut(s)	1530, 2024
Eco130I	CCWWGG	5 cut(s)	122, 520, 1407, 1678, 2140
Eco147I	AGGCCT	3 cut(s)	95, 1489, 2376
Eco31I	GGTCTC	1 cut(s)	1061
Eco47I	GGWCC	5 cut(s)	443, 739, 1193, 2812, 2829
Eco57I	CTGAAG	5 cut(s)	291, 524, 951, 1535, 1554
Eco81I	CCTNAGG	1 cut(s)	2359
Eco88I	CYCGRG	1 cut(s)	2856
EcoNI	CCTNNNNNAGG	2 cut(s)	2222, 2436
EcoO109I	RGGNCCY	1 cut(s)	739
EcoRII	CCWGG	5 cut(s)	892, 1048, 1189, 2417, 2942
EcoT14I	CCWWGG	5 cut(s)	122, 520, 1407, 1678, 2140
EgeI	GGCGCC	1 cut(s)	518
EheI	GGCGCC	1 cut(s)	518
ErhI	CCWWGG	5 cut(s)	122, 520, 1407, 1678, 2140
FalI	AAGNNNNNCTT	4 cut(s)	609, 641, 1671, 1703
FaqI	GGGAC	4 cut(s)	358, 1179, 1938, 2772
FauI	CCCGC	1 cut(s)	42
FauNDI	CATATG	1 cut(s)	1719
FokI	GGATG	6 cut(s)	525, 953, 1433, 2038, 2102, 2782
FspBI	CTAG	8 cut(s)	681, 1296, 1334, 1713, 2372, 2378, 2754, 2826
GlaI	GCGC	6 cut(s)	518, 579, 1878, 2176, 2510, 2750
GsuI	CTGGAG	2 cut(s)	1197, 1866
HaeII	RGCGCY	2 cut(s)	520, 2752
HaeIII	GGCC	9 cut(s)	95, 333, 427, 1159, 1350, 1489, 1541, 2209, 2376
HapII	CCGG	4 cut(s)	99, 441, 2023, 2695
HgaI	GACGC	1 cut(s)	1940
HhaI	GCGC	6 cut(s)	519, 580, 1879, 2177, 2511, 2751
Hin1I	GRCGYC	3 cut(s)	347, 517, 1951
Hin6I	GCGC	6 cut(s)	517, 578, 1877, 2175, 2509, 2749
HinP1I	GCGC	6 cut(s)	517, 578, 1877, 2175, 2509, 2749
HincII	GTYRAC	3 cut(s)	2164, 2194, 2215
HindII	GTYRAC	3 cut(s)	2164, 2194, 2215
HindIII	AAGCTT	1 cut(s)	1178
HpaII	CCGG	4 cut(s)	99, 441, 2023, 2695
HphI	GGTGA	6 cut(s)	55, 1607, 1950, 2712, 2789, 2810
Hpy166II	GTNNAC	7 cut(s)	231, 398, 1860, 2164, 2194, 2215, 2720
Hpy8I	GTNNAC	7 cut(s)	231, 398, 1860, 2164, 2194, 2215, 2720
Hpy99I	CGWCG	2 cut(s)	2204, 2907
HpyCH4III	ACNGT	8 cut(s)	211, 1374, 1824, 2095, 2330, 2777, 2816, 2822
HpyCH4IV	ACGT	5 cut(s)	347, 1975, 2199, 2902, 2932
HpyF3I	CTNAG	9 cut(s)	405, 636, 780, 813, 1698, 2359, 2391, 2526, 2922
HpySE526I	ACGT	5 cut(s)	347, 1975, 2199, 2902, 2932
Hsp92I	GRCGYC	3 cut(s)	347, 517, 1951
HspAI	GCGC	6 cut(s)	517, 578, 1877, 2175, 2509, 2749
KasI	GGCGCC	1 cut(s)	516
KroI	GCCGGC	1 cut(s)	2694
KroNI	GCCGGC	1 cut(s)	2696
LguI	GCTCTTC	1 cut(s)	2024
Lsp1109I	GCAGC	9 cut(s)	367, 486, 660, 1160, 1750, 1778, 2321, 2382, 2724
LweI	GCATC	7 cut(s)	553, 601, 916, 1085, 1590, 1762, 2902
MabI	ACCWGGT	1 cut(s)	1048
MaeI	CTAG	8 cut(s)	681, 1296, 1334, 1713, 2372, 2378, 2754, 2826
MaeII	ACGT	5 cut(s)	347, 1975, 2199, 2902, 2932
MaeIII	GTNAC	5 cut(s)	101, 2108, 2540, 2564, 2816
MbiI	CCGCTC	3 cut(s)	49, 1384, 1657
MflI	RGATCY	5 cut(s)	371, 1186, 1288, 2453, 2849
MhlI	GDGCHC	3 cut(s)	844, 913, 1741
MlsI	TGGCCA	1 cut(s)	1350
MluNI	TGGCCA	1 cut(s)	1350
Mly113I	GGCGCC	1 cut(s)	517
MlyI	GAGTC	4 cut(s)	626, 1520, 1709, 2909
MmeI	TCCRAC	2 cut(s)	1318, 2298
Mox20I	TGGCCA	1 cut(s)	1350
MroNI	GCCGGC	1 cut(s)	2694
MroXI	GAANNNNTTC	2 cut(s)	462, 1731
MscI	TGGCCA	1 cut(s)	1350
MseI	TTAA	4 cut(s)	1238, 2324, 2559, 2838
MslI	CAYNNNNRTG	2 cut(s)	2664, 2703
Msp20I	TGGCCA	1 cut(s)	1350
MspA1I	CMGCKG	3 cut(s)	194, 380, 1769
MspI	CCGG	4 cut(s)	99, 441, 2023, 2695
MspR9I	CCNGG	7 cut(s)	441, 894, 1050, 1191, 2023, 2419, 2944
MvaI	CCWGG	5 cut(s)	894, 1050, 1191, 2419, 2944
MvnI	CGCG	4 cut(s)	256, 580, 2509, 2539
NaeI	GCCGGC	1 cut(s)	2696
NarI	GGCGCC	1 cut(s)	517
NciI	CCSGG	2 cut(s)	441, 2023
NcoI	CCATGG	1 cut(s)	520
NdeI	CATATG	1 cut(s)	1719
NgoMIV	GCCGGC	1 cut(s)	2694
NlaIV	GGNNCC	8 cut(s)	518, 1047, 1195, 1849, 2385, 2535, 2699, 2851
NmeAIII	GCCGAG	1 cut(s)	115
NmuCI	GTSAC	3 cut(s)	2108, 2540, 2816
NspI	RCATGY	1 cut(s)	776
NspV	TTCGAA	2 cut(s)	1039, 2650
PaeI	GCATGC	1 cut(s)	776
PaeR7I	CTCGAG	1 cut(s)	2856
PceI	AGGCCT	3 cut(s)	95, 1489, 2376
PciSI	GCTCTTC	1 cut(s)	2024
PdiI	GCCGGC	1 cut(s)	2696
PdmI	GAANNNNTTC	2 cut(s)	462, 1731
PfeI	GAWTC	9 cut(s)	479, 539, 551, 2063, 2354, 2503, 2780, 2792, 2939
PflFI	GACNNNGTC	2 cut(s)	1247, 2198
PflMI	CCANNNNNTGG	1 cut(s)	340
PfoI	TCCNGGA	2 cut(s)	439, 2942
PleI	GAGTC	4 cut(s)	626, 1519, 1708, 2909
PluTI	GGCGCC	1 cut(s)	520
PpsI	GAGTC	4 cut(s)	626, 1519, 1708, 2909
PpuMI	RGGWCCY	1 cut(s)	739
PsiI	TTATAA	2 cut(s)	768, 948
Psp1406I	AACGTT	2 cut(s)	1975, 2932
Psp5II	RGGWCCY	1 cut(s)	739
Psp6I	CCWGG	5 cut(s)	892, 1048, 1189, 2417, 2942
PspGI	CCWGG	5 cut(s)	892, 1048, 1189, 2417, 2942
PspN4I	GGNNCC	8 cut(s)	518, 1047, 1195, 1849, 2385, 2535, 2699, 2851
PspPI	GGNCC	6 cut(s)	443, 739, 1157, 1193, 2812, 2829
PspPPI	RGGWCCY	1 cut(s)	739
PstI	CTGCAG	1 cut(s)	1768
PstNI	CAGNNNCTG	3 cut(s)	383, 452, 2066
PsuI	RGATCY	5 cut(s)	371, 1186, 1288, 2453, 2849
PsyI	GACNNNGTC	2 cut(s)	1247, 2198
PvuII	CAGCTG	3 cut(s)	194, 380, 1769
RsaI	GTAC	4 cut(s)	112, 1122, 1137, 1556
RsaNI	GTAC	4 cut(s)	111, 1121, 1136, 1555
RseI	CAYNNNNRTG	2 cut(s)	2664, 2703
SapI	GCTCTTC	1 cut(s)	2024
SaqAI	TTAA	4 cut(s)	1238, 2324, 2559, 2838
Sau96I	GGNCC	6 cut(s)	443, 739, 1157, 1193, 2812, 2829
SchI	GAGTC	4 cut(s)	626, 1520, 1709, 2909
ScrFI	CCNGG	7 cut(s)	441, 894, 1050, 1191, 2023, 2419, 2944
SduI	GDGCHC	3 cut(s)	844, 913, 1741
SexAI	ACCWGGT	1 cut(s)	1048
SfaNI	GCATC	7 cut(s)	553, 601, 916, 1085, 1590, 1762, 2902
SfcI	CTRYAG	4 cut(s)	375, 483, 1116, 1764
SfoI	GGCGCC	1 cut(s)	518
Sfr274I	CTCGAG	1 cut(s)	2856
SfuI	TTCGAA	2 cut(s)	1039, 2650
SinI	GGWCC	5 cut(s)	443, 739, 1193, 2812, 2829
SlaI	CTCGAG	1 cut(s)	2856
SmiMI	CAYNNNNRTG	2 cut(s)	2664, 2703
SmlI	CTYRAG	3 cut(s)	1664, 2689, 2856
SmoI	CTYRAG	3 cut(s)	1664, 2689, 2856
SphI	GCATGC	1 cut(s)	776
SseBI	AGGCCT	3 cut(s)	95, 1489, 2376
SsiI	CCGC	8 cut(s)	49, 236, 256, 1384, 1657, 2486, 2507, 2537
SspDI	GGCGCC	1 cut(s)	516
SspMI	CTAG	8 cut(s)	681, 1296, 1334, 1713, 2372, 2378, 2754, 2826
StuI	AGGCCT	3 cut(s)	95, 1489, 2376
StyD4I	CCNGG	7 cut(s)	439, 892, 1048, 1189, 2021, 2417, 2942
StyI	CCWWGG	5 cut(s)	122, 520, 1407, 1678, 2140
TaaI	ACNGT	8 cut(s)	211, 1374, 1824, 2095, 2330, 2777, 2816, 2822
TaiI	ACGT	5 cut(s)	350, 1978, 2202, 2905, 2935
TatI	WGTACW	1 cut(s)	1554
TauI	GCSGC	2 cut(s)	1387, 2488
TfiI	GAWTC	9 cut(s)	479, 539, 551, 2063, 2354, 2503, 2780, 2792, 2939
Tru1I	TTAA	4 cut(s)	1238, 2324, 2559, 2838
Tru9I	TTAA	4 cut(s)	1238, 2324, 2559, 2838
TscAI	CASTG	2 cut(s)	613, 1952
TseFI	GTSAC	3 cut(s)	2108, 2540, 2816
TseI	GCWGC	9 cut(s)	380, 499, 673, 1173, 1763, 1766, 2309, 2395, 2712
Tsp45I	GTSAC	3 cut(s)	2108, 2540, 2816
TspGWI	ACGGA	4 cut(s)	48, 73, 762, 1024
TspRI	CASTG	2 cut(s)	613, 1952
Tth111I	GACNNNGTC	2 cut(s)	1247, 2198
Van91I	CCANNNNNTGG	1 cut(s)	340
VpaK11BI	GGWCC	5 cut(s)	443, 739, 1193, 2812, 2829
XagI	CCTNNNNNAGG	2 cut(s)	2222, 2436
XapI	RAATTY	4 cut(s)	1148, 1472, 1903, 2520
XbaI	TCTAGA	1 cut(s)	1295
XceI	RCATGY	1 cut(s)	776
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1151, 1414
XhoI	CTCGAG	1 cut(s)	2856
XmnI	GAANNNNTTC	2 cut(s)	462, 1731
XspI	CTAG	8 cut(s)	681, 1296, 1334, 1713, 2372, 2378, 2754, 2826
ZraI	GACGTC	1 cut(s)	348

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.