MD14G1221200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010332
Physical Location & Seq
Reverse (-)
30291503 .. 30291994
492 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1221200.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGTTCGTCATCTGCATGCAAGCGCCGATTCCGACTCGGACTCGTCGATGCACCTGAAGTCCAAAACTCGTATGCTGGTCTCCATCTCGGCACTCCTTGCCATCGGCCGCCGCCTCTCGGGGAAGCTGAAAGCCACCAACTCCAAGCTGATAAAAAACAACAACCACTCGGATGTCTTGCCGAATAAGTCGCCGATGATGAGAAAGCCGAAGCAGCTGATGTCGACCATAAGCAACAAGGCCATCACCTTCCTGCACCTGAAAAAGATCGGCGAGGAATCGGATGGTAAGGCCCACGCCGCGGAGGAGGAGGAGTGGGGGGACGGTGGGGTATGGCAGAGGTCGATCCTGATGGGGGATAAGTGCGAGCCGTTGGATTTCTCGGGCGCCATTTACTACGATAGTAACGGGAAGAAACTTAACGGAGCTCCCCTCCGGTCCCCACGTGCAACTCCGATGCCGGGGTACCTCGAAAGGGTCAACCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

164

Amino Acids

18.03

Weight (kDa)

9.77

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 465
AccB1I GGYRCC 2 cut(s) 386, 465
AccI GTMKAC 1 cut(s) 224
AccII CGCG 1 cut(s) 302
AciI CCGC 4 cut(s) 109, 112, 300, 302
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 1 cut(s) 106
AcuI CTGAAG 1 cut(s) 77
AcvI CACGTG 1 cut(s) 446
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 1 cut(s) 467
AfiI CCNNNNNNNGG 5 cut(s) 118, 301, 355, 461, 475
AluBI AGCT 4 cut(s) 127, 148, 217, 428
AluI AGCT 4 cut(s) 127, 148, 217, 428
Alw21I GWGCWC 1 cut(s) 430
Alw26I GTCTC 1 cut(s) 85
AlwI GGATC 1 cut(s) 340
Ama87I CYCGRG 2 cut(s) 118, 382
AoxI GGCC 3 cut(s) 106, 240, 291
ApeKI GCWGC 1 cut(s) 214
Asp718I GGTACC 1 cut(s) 465
AspLEI GCGC 2 cut(s) 26, 389
AspS9I GGNCC 2 cut(s) 292, 438
AsuC2I CCSGG 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 238
AvaI CYCGRG 2 cut(s) 118, 382
AvaII GGWCC 1 cut(s) 438
BanI GGYRCC 2 cut(s) 386, 465
BanII GRGCYC 1 cut(s) 430
BbrPI CACGTG 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 430
BbvI GCAGC 1 cut(s) 226
BccI CCATC 5 cut(s) 92, 110, 251, 278, 346
BceAI ACGGC 1 cut(s) 355
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 1 cut(s) 85
BfoI RGCGCY 2 cut(s) 27, 390
BisI GCNGC 4 cut(s) 109, 112, 215, 300
BlsI GCNGC 4 cut(s) 110, 113, 216, 301
Bme1390I CCNGG 1 cut(s) 462
Bme18I GGWCC 1 cut(s) 438
BmeT110I CYCGRG 2 cut(s) 118, 382
BmgT120I GGNCC 2 cut(s) 292, 438
BmiI GGNNCC 3 cut(s) 388, 440, 467
BmrFI CCNGG 1 cut(s) 462
BmsI GCATC 2 cut(s) 39, 447
BplI GAGNNNNNCTC 2 cut(s) 417, 449
BpuMI CCSGG 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 446
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 85
BsaJI CCNNGG 2 cut(s) 300, 461
BsaWI WCCGGW 1 cut(s) 435
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bsc4I CCNNNNNNNGG 5 cut(s) 118, 301, 355, 461, 475
BseDI CCNNGG 2 cut(s) 300, 461
BseGI GGATG 2 cut(s) 178, 289
BseLI CCNNNNNNNGG 5 cut(s) 118, 301, 355, 461, 475
BseRI GAGGAG 3 cut(s) 320, 323, 326
BseX3I CGGCCG 1 cut(s) 106
BseXI GCAGC 1 cut(s) 226
BsgI GTGCAG 1 cut(s) 239
Bsh1236I CGCG 1 cut(s) 302
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 108, 242, 293
BshNI GGYRCC 2 cut(s) 386, 465
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 430
BsiHKCI CYCGRG 2 cut(s) 118, 382
BsiSI CCGG 2 cut(s) 436, 461
BslFI GGGAC 2 cut(s) 335, 424
BslI CCNNNNNNNGG 5 cut(s) 118, 301, 355, 461, 475
BsmAI GTCTC 1 cut(s) 85
BsmFI GGGAC 2 cut(s) 335, 424
BsnI GGCC 3 cut(s) 108, 242, 293
Bso31I GGTCTC 1 cut(s) 85
BsoBI CYCGRG 2 cut(s) 118, 382
Bsp1286I GDGCHC 1 cut(s) 430
Bsp143I GATC 2 cut(s) 267, 345
BspACI CCGC 4 cut(s) 109, 112, 300, 302
BspANI GGCC 3 cut(s) 108, 242, 293
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 3 cut(s) 388, 440, 467
BspPI GGATC 1 cut(s) 340
BspT107I GGYRCC 2 cut(s) 386, 465
BspTNI GGTCTC 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 300, 461
BssMI GATC 2 cut(s) 267, 345
BssNI GRCGYC 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 326
BstACI GRCGYC 1 cut(s) 387
BstAPI GCANNNNNTGC 1 cut(s) 98
BstBAI YACGTR 1 cut(s) 446
BstC8I GCNNGC 3 cut(s) 18, 22, 368
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 2 cut(s) 178, 289
BstFNI CGCG 1 cut(s) 302
BstH2I RGCGCY 2 cut(s) 27, 390
BstHHI GCGC 2 cut(s) 26, 389
BstKTI GATC 2 cut(s) 270, 348
BstMAI GTCTC 1 cut(s) 85
BstMBI GATC 2 cut(s) 267, 345
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 3 cut(s) 98, 214, 299
BstNSI RCATGY 1 cut(s) 20
BstSCI CCNGG 1 cut(s) 460
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 226
BstZI CGGCCG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 108, 242, 293
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 2 cut(s) 178, 289
Cac8I GCNNGC 3 cut(s) 18, 22, 368
CfoI GCGC 2 cut(s) 26, 389
Cfr13I GGNCC 2 cut(s) 292, 438
Cfr42I CCGCGG 1 cut(s) 303
Csp6I GTAC 1 cut(s) 466
CviAII CATG 1 cut(s) 17
CviQI GTAC 1 cut(s) 466
DinI GGCGCC 1 cut(s) 388
DpnI GATC 2 cut(s) 269, 347
DpnII GATC 2 cut(s) 267, 345
EaeI YGGCCR 1 cut(s) 106
EagI CGGCCG 1 cut(s) 106
Ecl136II GAGCTC 1 cut(s) 428
EclXI CGGCCG 1 cut(s) 106
Eco24I GRGCYC 1 cut(s) 430
Eco31I GGTCTC 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 438
Eco52I CGGCCG 1 cut(s) 106
Eco53kI GAGCTC 1 cut(s) 428
Eco57I CTGAAG 1 cut(s) 77
Eco72I CACGTG 1 cut(s) 446
Eco88I CYCGRG 2 cut(s) 118, 382
EcoICRI GAGCTC 1 cut(s) 428
EcoT38I GRGCYC 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 388
EheI GGCGCC 1 cut(s) 388
FaeI CATG 1 cut(s) 20
FaiI YATR 4 cut(s) 18, 74, 230, 334
FaqI GGGAC 2 cut(s) 335, 424
FatI CATG 1 cut(s) 16
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 4 cut(s) 109, 112, 215, 300
FokI GGATG 2 cut(s) 185, 296
FriOI GRGCYC 1 cut(s) 430
Fsp4HI GCNGC 4 cut(s) 109, 112, 215, 300
GlaI GCGC 2 cut(s) 25, 388
GluI GCNGC 4 cut(s) 109, 112, 215, 300
HaeII RGCGCY 2 cut(s) 27, 390
HaeIII GGCC 3 cut(s) 108, 242, 293
HapII CCGG 2 cut(s) 436, 461
HhaI GCGC 2 cut(s) 26, 389
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 1 cut(s) 20
Hin6I GCGC 2 cut(s) 24, 387
HinP1I GCGC 2 cut(s) 24, 387
HincII GTYRAC 2 cut(s) 225, 481
HindII GTYRAC 2 cut(s) 225, 481
HinfI GANTC 4 cut(s) 29, 35, 41, 278
HpaII CCGG 2 cut(s) 436, 461
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 2 cut(s) 225, 481
Hpy188I TCNGA 5 cut(s) 34, 40, 172, 283, 456
Hpy188III TCNNGA 1 cut(s) 349
Hpy8I GTNNAC 2 cut(s) 225, 481
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 1 cut(s) 259
HpyCH4III ACNGT 1 cut(s) 326
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 5 cut(s) 16, 20, 52, 256, 449
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 214, 299
HpySE526I ACGT 1 cut(s) 445
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 1 cut(s) 20
HspAI GCGC 2 cut(s) 24, 387
KasI GGCGCC 1 cut(s) 386
KpnI GGTACC 1 cut(s) 469
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 2 cut(s) 267, 345
LmnI GCTCC 2 cut(s) 425, 433
LpnPI CCDG 7 cut(s) 62, 68, 266, 272, 362, 449, 474
Lsp1109I GCAGC 1 cut(s) 226
LweI GCATC 2 cut(s) 39, 447
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 1 cut(s) 404
MalI GATC 2 cut(s) 269, 347
MboI GATC 2 cut(s) 267, 345
MboII GAAGA 1 cut(s) 424
MhlI GDGCHC 1 cut(s) 430
Mly113I GGCGCC 1 cut(s) 387
MlyI GAGTC 2 cut(s) 29, 35
MmeI TCCRAC 2 cut(s) 57, 354
MnlI CCTC 8 cut(s) 125, 268, 298, 301, 304, 333, 443, 479
MseI TTAA 1 cut(s) 420
MslI CAYNNNNRTG 2 cut(s) 15, 171
MspA1I CMGCKG 2 cut(s) 217, 302
MspI CCGG 2 cut(s) 436, 461
MspR9I CCNGG 1 cut(s) 462
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 3 cut(s) 98, 214, 299
NarI GGCGCC 1 cut(s) 387
NciI CCSGG 1 cut(s) 462
NdeII GATC 2 cut(s) 267, 345
NlaIII CATG 1 cut(s) 20
NlaIV GGNNCC 3 cut(s) 388, 440, 467
NmeAIII GCCGAG 1 cut(s) 68
NspI RCATGY 1 cut(s) 20
PaeI GCATGC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 2 cut(s) 29, 278
PkrI GCNGC 4 cut(s) 110, 113, 216, 301
PleI GAGTC 2 cut(s) 29, 35
PluTI GGCGCC 1 cut(s) 390
PmaCI CACGTG 1 cut(s) 446
PmlI CACGTG 1 cut(s) 446
PpsI GAGTC 2 cut(s) 29, 35
Ppu21I YACGTR 1 cut(s) 446
Psp124BI GAGCTC 1 cut(s) 430
PspCI CACGTG 1 cut(s) 446
PspN4I GGNNCC 3 cut(s) 388, 440, 467
PspPI GGNCC 2 cut(s) 292, 438
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 1 cut(s) 467
RsaNI GTAC 1 cut(s) 466
RseI CAYNNNNRTG 2 cut(s) 15, 171
SacI GAGCTC 1 cut(s) 430
SacII CCGCGG 1 cut(s) 303
SalI GTCGAC 1 cut(s) 223
SaqAI TTAA 1 cut(s) 420
SatI GCNGC 4 cut(s) 109, 112, 215, 300
Sau3AI GATC 2 cut(s) 267, 345
Sau96I GGNCC 2 cut(s) 292, 438
SchI GAGTC 2 cut(s) 29, 35
ScrFI CCNGG 1 cut(s) 462
SduI GDGCHC 1 cut(s) 430
SfaNI GCATC 2 cut(s) 39, 447
SfoI GGCGCC 1 cut(s) 388
Sfr303I CCGCGG 1 cut(s) 303
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 1 cut(s) 438
SmiMI CAYNNNNRTG 2 cut(s) 15, 171
SphI GCATGC 1 cut(s) 20
SsiI CCGC 4 cut(s) 109, 112, 300, 302
SspDI GGCGCC 1 cut(s) 386
SstI GAGCTC 1 cut(s) 430
StyD4I CCNGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 326
TaiI ACGT 1 cut(s) 448
TaqI TCGA 4 cut(s) 47, 224, 344, 471
TauI GCSGC 3 cut(s) 111, 114, 302
TfiI GAWTC 2 cut(s) 29, 278
Tru1I TTAA 1 cut(s) 420
Tru9I TTAA 1 cut(s) 420
TseI GCWGC 1 cut(s) 214
TspGWI ACGGA 1 cut(s) 438
VpaK11BI GGWCC 1 cut(s) 438
XceI RCATGY 1 cut(s) 20
XmiI GTMKAC 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.