RLG00000004970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010332
Physical Location & Seq
Forward (+)
64091508 .. 64091969
462 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004970

Sequence Viewer

Length: 462 bp
ATGGTGCGTCGGCTCCGAACAAACTCCGAAGTCGACTCAGACAGCTATCCGATACGGCTGAGCTCGAAGGCGAGGTTGCTTGCCTCCGTCTCGGCCCTCATAGACATAGCCAGCCGCCTAACCCATAAGCTCAAGGCCAAGCCTATGATCAAGACGTCGTCGTTGAACAGGCCCAAGCAGCTGATGGCGACCATAAGCAACAAGGCCATCACGTTCATGCACCGGAAGAAGGTGGCCGGCGGCGGTGCGGGACCCAAGGGTGGTGGGACCCTTGAGGAGGATGAGTGGGGAGACGGCGGGGTGTGGCAGCGGTCGATTCTGATGGGGGACAAGTGCGAGCCGTTGGATTTCTCGGGCGCAATATATTATGATAGTAAGGGGCAGCAGCTGAATGGGGCTCCTCTCAGGTCGCCACGTGCGAGTCCCTTGCCAGGGTACCTGGAGAGGGCTCAGAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

16.68

Weight (kDa)

10.05

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 158
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccI GTMKAC 1 cut(s) 33
AciI CCGC 6 cut(s) 115, 240, 243, 248, 297, 310
AcoI YGGCCR 1 cut(s) 234
AcvI CACGTG 1 cut(s) 416
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 1 cut(s) 437
AfiI CCNNNNNNNGG 6 cut(s) 229, 260, 277, 431, 432, 445
AgsI TTSAA 1 cut(s) 166
AjnI CCWGG 2 cut(s) 430, 438
AluBI AGCT 5 cut(s) 45, 63, 130, 181, 388
AluI AGCT 5 cut(s) 45, 63, 130, 181, 388
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 2 cut(s) 94, 285
AlwNI CAGNNNCTG 1 cut(s) 388
Ama87I CYCGRG 1 cut(s) 352
AoxI GGCC 5 cut(s) 93, 135, 170, 204, 234
ApeKI GCWGC 4 cut(s) 178, 307, 382, 385
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 1 cut(s) 359
AspS9I GGNCC 4 cut(s) 94, 171, 251, 267
AvaI CYCGRG 1 cut(s) 352
AvaII GGWCC 2 cut(s) 251, 267
BanI GGYRCC 1 cut(s) 435
BanII GRGCYC 3 cut(s) 65, 400, 451
BbrPI CACGTG 1 cut(s) 416
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 4 cut(s) 190, 319, 394, 397
BccI CCATC 3 cut(s) 178, 215, 316
BceAI ACGGC 3 cut(s) 71, 310, 325
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BciT130I CCWGG 2 cut(s) 432, 440
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 94, 285
BisI GCNGC 6 cut(s) 115, 179, 241, 308, 383, 386
BlpI GCTNAGC 1 cut(s) 59
BlsI GCNGC 6 cut(s) 116, 180, 242, 309, 384, 387
Bme1390I CCNGG 2 cut(s) 432, 440
Bme18I GGWCC 2 cut(s) 251, 267
BmeT110I CYCGRG 1 cut(s) 352
BmgT120I GGNCC 4 cut(s) 94, 171, 251, 267
BmiI GGNNCC 7 cut(s) 14, 252, 253, 268, 269, 399, 437
BmrFI CCNGG 2 cut(s) 432, 440
BpmI CTGGAG 1 cut(s) 461
Bpu1102I GCTNAGC 1 cut(s) 59
BpuEI CTTGAG 2 cut(s) 116, 293
BsaAI YACGTR 1 cut(s) 416
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 2 cut(s) 255, 431
BsaWI WCCGGW 1 cut(s) 222
BsaXI ACNNNNNCTCC 2 cut(s) 269, 299
Bsc4I CCNNNNNNNGG 6 cut(s) 229, 260, 277, 431, 432, 445
Bse118I RCCGGY 1 cut(s) 236
BseBI CCWGG 2 cut(s) 432, 440
BseDI CCNNGG 2 cut(s) 255, 431
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 6 cut(s) 229, 260, 277, 431, 432, 445
BseMII CTCAG 3 cut(s) 50, 51, 418
BseRI GAGGAG 2 cut(s) 290, 390
BseXI GCAGC 4 cut(s) 190, 319, 394, 397
Bsh1285I CGRYCG 1 cut(s) 314
BshFI GGCC 5 cut(s) 95, 137, 172, 206, 236
BshNI GGYRCC 1 cut(s) 435
BsiEI CGRYCG 1 cut(s) 314
BsiHKAI GWGCWC 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 352
BsiSI CCGG 2 cut(s) 223, 237
BslFI GGGAC 4 cut(s) 264, 280, 341, 408
BslI CCNNNNNNNGG 6 cut(s) 229, 260, 277, 431, 432, 445
BsmAI GTCTC 2 cut(s) 94, 285
BsmBI CGTCTC 2 cut(s) 94, 285
BsmFI GGGAC 4 cut(s) 264, 280, 341, 408
BsnI GGCC 5 cut(s) 95, 137, 172, 206, 236
BsoBI CYCGRG 1 cut(s) 352
Bsp1286I GDGCHC 3 cut(s) 65, 400, 451
Bsp143I GATC 1 cut(s) 147
Bsp1720I GCTNAGC 1 cut(s) 59
BspACI CCGC 6 cut(s) 115, 240, 243, 248, 297, 310
BspANI GGCC 5 cut(s) 95, 137, 172, 206, 236
BspCNI CTCAG 3 cut(s) 50, 51, 417
BspLI GGNNCC 7 cut(s) 14, 252, 253, 268, 269, 399, 437
BspT107I GGYRCC 1 cut(s) 435
BsrFI RCCGGY 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 2 cut(s) 255, 431
BssMI GATC 1 cut(s) 147
BssNI GRCGYC 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 255
Bst2UI CCWGG 2 cut(s) 432, 440
BstACI GRCGYC 1 cut(s) 155
BstBAI YACGTR 1 cut(s) 416
BstC8I GCNNGC 4 cut(s) 81, 112, 238, 338
BstDEI CTNAG 4 cut(s) 37, 59, 404, 450
BstENI CCTNNNNNAGG 1 cut(s) 275
BstF5I GGATG 1 cut(s) 286
BstHHI GCGC 1 cut(s) 359
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 2 cut(s) 94, 285
BstMBI GATC 1 cut(s) 147
BstMCI CGRYCG 1 cut(s) 314
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstNI CCWGG 2 cut(s) 432, 440
BstSCI CCNGG 2 cut(s) 430, 438
BstV1I GCAGC 4 cut(s) 190, 319, 394, 397
BsuRI GGCC 5 cut(s) 95, 137, 172, 206, 236
BtsCI GGATG 1 cut(s) 286
Cac8I GCNNGC 4 cut(s) 81, 112, 238, 338
CaiI CAGNNNCTG 1 cut(s) 388
CfoI GCGC 1 cut(s) 359
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 4 cut(s) 94, 171, 251, 267
Csp6I GTAC 1 cut(s) 436
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 1 cut(s) 217
CviQI GTAC 1 cut(s) 436
DdeI CTNAG 4 cut(s) 37, 59, 404, 450
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
EaeI YGGCCR 1 cut(s) 234
Ecl136II GAGCTC 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 3 cut(s) 65, 400, 451
Eco47I GGWCC 2 cut(s) 251, 267
Eco53kI GAGCTC 1 cut(s) 63
Eco72I CACGTG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 352
EcoICRI GAGCTC 1 cut(s) 63
EcoNI CCTNNNNNAGG 1 cut(s) 275
EcoO109I RGGNCCY 2 cut(s) 251, 267
EcoRII CCWGG 2 cut(s) 430, 438
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 3 cut(s) 65, 400, 451
ErhI CCWWGG 1 cut(s) 255
Esp3I CGTCTC 2 cut(s) 94, 285
FaeI CATG 1 cut(s) 220
FaiI YATR 8 cut(s) 101, 107, 126, 146, 194, 218, 364, 369
FaqI GGGAC 4 cut(s) 264, 280, 341, 408
FatI CATG 1 cut(s) 216
FauI CCCGC 2 cut(s) 241, 290
FbaI TGATCA 1 cut(s) 147
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 6 cut(s) 115, 179, 241, 308, 383, 386
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 3 cut(s) 65, 400, 451
Fsp4HI GCNGC 6 cut(s) 115, 179, 241, 308, 383, 386
GlaI GCGC 1 cut(s) 358
GluI GCNGC 6 cut(s) 115, 179, 241, 308, 383, 386
GsuI CTGGAG 1 cut(s) 461
HaeIII GGCC 5 cut(s) 95, 137, 172, 206, 236
HapII CCGG 2 cut(s) 223, 237
HhaI GCGC 1 cut(s) 359
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 357
HinP1I GCGC 1 cut(s) 357
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 3 cut(s) 35, 316, 421
HpaII CCGG 2 cut(s) 223, 237
Hpy166II GTNNAC 1 cut(s) 34
Hpy188I TCNGA 7 cut(s) 17, 28, 40, 51, 321, 453, 461
Hpy188III TCNNGA 1 cut(s) 151
Hpy8I GTNNAC 1 cut(s) 34
Hpy99I CGWCG 3 cut(s) 12, 160, 163
HpyAV CCTTC 2 cut(s) 61, 223
HpyCH4IV ACGT 3 cut(s) 155, 212, 415
HpyCH4V TGCA 1 cut(s) 220
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 4 cut(s) 37, 59, 404, 450
HpySE526I ACGT 3 cut(s) 155, 212, 415
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 357
KflI GGGWCCC 2 cut(s) 251, 267
KpnI GGTACC 1 cut(s) 439
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LmnI GCTCC 2 cut(s) 18, 403
LpnPI CCDG 9 cut(s) 124, 154, 236, 250, 391, 417, 425, 444, 452
Lsp1109I GCAGC 4 cut(s) 190, 319, 394, 397
MaeII ACGT 3 cut(s) 155, 212, 415
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 1 cut(s) 238
MhlI GDGCHC 3 cut(s) 65, 400, 451
MlyI GAGTC 2 cut(s) 29, 430
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 7 cut(s) 66, 94, 107, 268, 271, 411, 438
MroNI GCCGGC 1 cut(s) 236
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 3 cut(s) 181, 310, 388
MspI CCGG 2 cut(s) 223, 237
MspR9I CCNGG 2 cut(s) 432, 440
MvaI CCWGG 2 cut(s) 432, 440
MwoI GCNNNNNNNGC 1 cut(s) 178
NaeI GCCGGC 1 cut(s) 238
NdeII GATC 1 cut(s) 147
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 7 cut(s) 14, 252, 253, 268, 269, 399, 437
NmeAIII GCCGAG 1 cut(s) 71
PcsI WCGNNNNNNNCGW 1 cut(s) 416
PdiI GCCGGC 1 cut(s) 238
PfeI GAWTC 1 cut(s) 316
PflFI GACNNNGTC 1 cut(s) 157
PkrI GCNGC 6 cut(s) 116, 180, 242, 309, 384, 387
PleI GAGTC 2 cut(s) 29, 429
PmaCI CACGTG 1 cut(s) 416
PmlI CACGTG 1 cut(s) 416
PpsI GAGTC 2 cut(s) 29, 429
Ppu21I YACGTR 1 cut(s) 416
PpuMI RGGWCCY 2 cut(s) 251, 267
Psp124BI GAGCTC 1 cut(s) 65
Psp5II RGGWCCY 2 cut(s) 251, 267
Psp6I CCWGG 2 cut(s) 430, 438
PspCI CACGTG 1 cut(s) 416
PspGI CCWGG 2 cut(s) 430, 438
PspN4I GGNNCC 7 cut(s) 14, 252, 253, 268, 269, 399, 437
PspPI GGNCC 4 cut(s) 94, 171, 251, 267
PspPPI RGGWCCY 2 cut(s) 251, 267
PstNI CAGNNNCTG 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 157
PvuII CAGCTG 2 cut(s) 181, 388
RsaI GTAC 1 cut(s) 437
RsaNI GTAC 1 cut(s) 436
RseI CAYNNNNRTG 1 cut(s) 215
SacI GAGCTC 1 cut(s) 65
SalI GTCGAC 1 cut(s) 32
SatI GCNGC 6 cut(s) 115, 179, 241, 308, 383, 386
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 4 cut(s) 94, 171, 251, 267
SchI GAGTC 2 cut(s) 29, 430
ScrFI CCNGG 2 cut(s) 432, 440
SduI GDGCHC 3 cut(s) 65, 400, 451
SinI GGWCC 2 cut(s) 251, 267
SmiMI CAYNNNNRTG 1 cut(s) 215
SmlI CTYRAG 2 cut(s) 131, 272
SmoI CTYRAG 2 cut(s) 131, 272
SsiI CCGC 6 cut(s) 115, 240, 243, 248, 297, 310
SstI GAGCTC 1 cut(s) 65
StyD4I CCNGG 2 cut(s) 430, 438
StyI CCWWGG 1 cut(s) 255
TaiI ACGT 3 cut(s) 158, 215, 418
TaqI TCGA 3 cut(s) 33, 65, 314
TauI GCSGC 2 cut(s) 117, 243
TfiI GAWTC 1 cut(s) 316
TseI GCWGC 4 cut(s) 178, 307, 382, 385
TspDTI ATGAA 1 cut(s) 205
TspGWI ACGGA 1 cut(s) 76
Tth111I GACNNNGTC 1 cut(s) 157
VpaK11BI GGWCC 2 cut(s) 251, 267
XagI CCTNNNNNAGG 1 cut(s) 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 181
XmiI GTMKAC 1 cut(s) 33
ZraI GACGTC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.