MD14G1226900.v1.1

No apical meristem (NAM) protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015159
Physical Location & Seq
Forward (+)
30818615 .. 30821205
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1226900.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGCAAGAGAGTCTTCCTCCAGGGTTCAGATTCCATCCTACAGATGAAGAACTGATAACGCACTATCTGTCCCACAAGGTTTCCGATGTTAGTTTCACGTCGAAAGCAGTAGCAGTCGTTGATCTTAATAAGTGTGAACCTTGGGACCTCCCAGGCAAGGCTTCGATGGGAGAGAAAGAATGGTACTTCTTCAACCTGAGAGACCGAAAATATCCAACTGGACTCCGAACCAACCGAGCCACCGAAGCCGGCTACTGGAAAACAACTGGGAAAGACAAGGAAATACGTCGTGTAGGTGTGCTGGTTGGGATGAAGAAAACCCTAGTTTTCTACAAGGGGAGAGCTCCAAGGGGTGAGAAAAGCAACTGGGTCATGCATGAATACAGACTAGAAAACAAGCATCCCTTCAAATCCTCAAAGGAAGAATGGGTGGTTTGTAGGGTTTTCCAAAAAAACATTAGTGCAGTGAAAAAGCCACAACAAGCAACAACATCCTCACAGAAGCAGCCAACCAACGAGTCTCCATGCGACGATACGAACTCAATTGTTAACGAATTCGGAGACATCGAGCTGCCAAACCTAAACAGCAATATTAACGCAAATTCATCCAGCGGGTTCAGCAACAACATTTCTTCACCCCAAAGCTATTACAGCAATATTCTTGATCACAGCAAGAACAACATAGACGACGTTACCAACATGAGTTTGAAAATGACTTGGGCAGCAACAGCGGCAAGAGAGGCAGCCGCCAACGCTCTTTCATTGCCGTCAAGCTTGCTAAGTCCCTCGAATCTTTCGGTGAATTCGTTGCTTCTTAGGGCATTACAACTTAGGAGTAGTACTAATAATAATGGTAGTAATTATCAACCAAGAGCTACTAATCATGTTCAAGCTACAGCAGGCACTAGTACTCTAGATTACTCATCATCATATAATACTATCCAGCAGCAAGGAATGATGCCGTCAATGTCTCATAATCACTTTGGATCCAGTACTACTATGGATCATATAAATTCAAATTTCCAAGCTTCTTCGTCGAGTACTAATGATCAGATGATGGAATCGATGCCGCTGCAGCATTCGGAGCAACCATTCAATTTGGACTCCATTTGGTGA

Protein Analysis

372

Amino Acids

41.56

Weight (kDa)

8.28

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 6 - 130 2.7e-48 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015159)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18400
fragaria_vesca FvH4_5g12090
malus_domestica MD06G1216500.v1.1 MD14G1226900.v1.1
prunus_persica Prupe.5G221600_v2.0.a1
pyrus_communis pycom06g19290 pycom14g18830
rosa_chinensis RchiOBHm_Chr7g0185481
rosa_laevigata RLG00000004909
rosa_multiflora Rmu_sc0010190.1_g000022
rosa_roxburghii Rroxscaffold_3G00268900
rosa_rugosa Rorug06G0469800
rosa_samantha Rh7AG073100 Rh7BG074500 Rh7CG074600
rosa_wichuraiana Rw7G006200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 612, 731, 747, 1070
AclWI GGATC 3 cut(s) 981, 994, 1011
AcsI RAATTY 5 cut(s) 554, 601, 802, 1012, 1018
AfaI GTAC 5 cut(s) 185, 841, 910, 994, 1042
AfiI CCNNNNNNNGG 2 cut(s) 157, 255
AgsI TTSAA 6 cut(s) 193, 409, 709, 890, 1017, 1096
AhlI ACTAGT 1 cut(s) 905
AjiI CACGTC 1 cut(s) 99
AjnI CCWGG 2 cut(s) 19, 151
AluBI AGCT 7 cut(s) 344, 571, 645, 774, 875, 893, 1028
AluI AGCT 7 cut(s) 344, 571, 645, 774, 875, 893, 1028
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 4 cut(s) 195, 525, 555, 975
AlwI GGATC 3 cut(s) 981, 994, 1011
ApeKI GCWGC 7 cut(s) 505, 571, 722, 743, 946, 1072, 1075
ApoI RAATTY 5 cut(s) 554, 601, 802, 1012, 1018
AspS9I GGNCC 1 cut(s) 145
AsuHPI GGTGA 3 cut(s) 365, 627, 811
AvaII GGWCC 1 cut(s) 145
BamHI GGATCC 1 cut(s) 986
BanII GRGCYC 1 cut(s) 346
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 7 cut(s) 517, 558, 734, 755, 958, 1059, 1087
BccI CCATC 3 cut(s) 42, 160, 1051
BceAI ACGGC 2 cut(s) 751, 946
BcgI CGANNNNNNTGC 2 cut(s) 1054, 1088
BciT130I CCWGG 2 cut(s) 21, 153
BclI TGATCA 2 cut(s) 664, 1048
BcoDI GTCTC 4 cut(s) 195, 525, 555, 975
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 4 cut(s) 323, 389, 906, 914
BfmI CTRYAG 3 cut(s) 39, 894, 1073
BmcAI AGTACT 4 cut(s) 841, 910, 994, 1042
Bme1390I CCNGG 2 cut(s) 21, 153
Bme18I GGWCC 1 cut(s) 145
BmgBI CACGTC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 145
BmiI GGNNCC 2 cut(s) 146, 988
BmrFI CCNGG 2 cut(s) 21, 153
BmrI ACTGGG 2 cut(s) 276, 376
BmsI GCATC 3 cut(s) 409, 948, 1056
BmuI ACTGGG 2 cut(s) 276, 376
BpiI GAAGAC 1 cut(s) 5
BplI GAGNNNNNCTC 1 cut(s) 33
Bsa29I ATCGAT 1 cut(s) 1064
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 4 cut(s) 20, 140, 151, 347
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 255
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 5 cut(s) 223, 260, 271, 371, 990
Bse3DI GCAATG 1 cut(s) 761
BseBI CCWGG 2 cut(s) 21, 153
BseCI ATCGAT 1 cut(s) 1064
BseDI CCNNGG 4 cut(s) 20, 140, 151, 347
BseGI GGATG 5 cut(s) 34, 315, 400, 491, 605
BseLI CCNNNNNNNGG 2 cut(s) 157, 255
BseMI GCAATG 1 cut(s) 761
BseMII CTCAG 1 cut(s) 188
BseNI ACTGG 5 cut(s) 223, 260, 271, 371, 990
BseXI GCAGC 7 cut(s) 517, 558, 734, 755, 958, 1059, 1087
BsgI GTGCAG 1 cut(s) 483
BshVI ATCGAT 1 cut(s) 1064
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 249
BslFI GGGAC 3 cut(s) 55, 158, 768
BslI CCNNNNNNNGG 2 cut(s) 157, 255
BsmAI GTCTC 4 cut(s) 195, 525, 555, 975
BsmFI GGGAC 3 cut(s) 55, 158, 768
BsmI GAATGC 1 cut(s) 1078
Bso31I GGTCTC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 5 cut(s) 121, 664, 986, 1003, 1048
BspACI CCGC 4 cut(s) 612, 731, 747, 1070
BspCNI CTCAG 1 cut(s) 189
BspDI ATCGAT 1 cut(s) 1064
BspLI GGNNCC 2 cut(s) 146, 988
BspMAI CTGCAG 1 cut(s) 1077
BspPI GGATC 3 cut(s) 981, 994, 1011
BspTNI GGTCTC 1 cut(s) 195
BsrDI GCAATG 1 cut(s) 761
BsrFI RCCGGY 1 cut(s) 248
BsrI ACTGG 5 cut(s) 223, 260, 271, 371, 990
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 4 cut(s) 20, 140, 151, 347
BssMI GATC 5 cut(s) 121, 664, 986, 1003, 1048
BssT1I CCWWGG 2 cut(s) 140, 347
Bst2UI CCWGG 2 cut(s) 21, 153
BstC8I GCNNGC 3 cut(s) 250, 776, 901
BstDEI CTNAG 4 cut(s) 197, 779, 815, 830
BstF5I GGATG 5 cut(s) 34, 315, 400, 491, 605
BstKTI GATC 5 cut(s) 124, 667, 989, 1006, 1051
BstMAI GTCTC 4 cut(s) 195, 525, 555, 975
BstMBI GATC 5 cut(s) 121, 664, 986, 1003, 1048
BstMWI GCNNNNNNNGC 9 cut(s) 245, 618, 651, 728, 731, 740, 752, 1075, 1084
BstNI CCWGG 2 cut(s) 21, 153
BstSCI CCNGG 2 cut(s) 19, 151
BstSFI CTRYAG 3 cut(s) 39, 894, 1073
BstV1I GCAGC 7 cut(s) 517, 558, 734, 755, 958, 1059, 1087
BstV2I GAAGAC 1 cut(s) 5
BstX2I RGATCY 1 cut(s) 986
BstYI RGATCY 1 cut(s) 986
Bsu15I ATCGAT 1 cut(s) 1064
BsuTUI ATCGAT 1 cut(s) 1064
BtrI CACGTC 1 cut(s) 99
BtsCI GGATG 5 cut(s) 34, 315, 400, 491, 605
BtsI GCAGTG 1 cut(s) 471
BtsIMutI CAGTG 1 cut(s) 471
Cac8I GCNNGC 3 cut(s) 250, 776, 901
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 1064
Csp6I GTAC 5 cut(s) 184, 840, 909, 993, 1041
CviAII CATG 5 cut(s) 373, 377, 525, 700, 884
CviQI GTAC 5 cut(s) 184, 840, 909, 993, 1041
DdeI CTNAG 4 cut(s) 197, 779, 815, 830
DpnI GATC 5 cut(s) 123, 666, 988, 1005, 1050
DpnII GATC 5 cut(s) 121, 664, 986, 1003, 1048
Ecl136II GAGCTC 1 cut(s) 344
Eco130I CCWWGG 2 cut(s) 140, 347
Eco24I GRGCYC 1 cut(s) 346
Eco31I GGTCTC 1 cut(s) 195
Eco47I GGWCC 1 cut(s) 145
Eco53kI GAGCTC 1 cut(s) 344
EcoICRI GAGCTC 1 cut(s) 344
EcoO109I RGGNCCY 1 cut(s) 145
EcoRI GAATTC 2 cut(s) 554, 802
EcoRII CCWGG 2 cut(s) 19, 151
EcoT14I CCWWGG 2 cut(s) 140, 347
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 1 cut(s) 346
ErhI CCWWGG 2 cut(s) 140, 347
FaeI CATG 5 cut(s) 376, 380, 528, 703, 887
FalI AAGNNNNNCTT 3 cut(s) 29, 389, 421
FaqI GGGAC 3 cut(s) 55, 158, 768
FatI CATG 5 cut(s) 372, 376, 524, 699, 883
FauI CCCGC 1 cut(s) 605
FbaI TGATCA 2 cut(s) 664, 1048
FokI GGATG 5 cut(s) 21, 322, 387, 478, 592
FriOI GRGCYC 1 cut(s) 346
FspBI CTAG 4 cut(s) 323, 389, 906, 914
HapII CCGG 1 cut(s) 249
Hin1II CATG 5 cut(s) 376, 380, 528, 703, 887
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 2 cut(s) 772, 1026
HinfI GANTC 7 cut(s) 10, 30, 222, 518, 790, 1061, 1103
HpaI GTTAAC 1 cut(s) 550
HpaII CCGG 1 cut(s) 249
HphI GGTGA 3 cut(s) 365, 627, 811
Hpy166II GTNNAC 2 cut(s) 137, 550
Hpy188I TCNGA 6 cut(s) 29, 85, 227, 560, 1053, 1084
Hpy188III TCNNGA 2 cut(s) 662, 914
Hpy8I GTNNAC 2 cut(s) 137, 550
Hpy99I CGWCG 5 cut(s) 103, 291, 533, 692, 1039
HpyAV CCTTC 1 cut(s) 415
HpyCH4IV ACGT 3 cut(s) 98, 286, 690
HpyCH4V TGCA 4 cut(s) 4, 376, 464, 1075
HpyF10VI GCNNNNNNNGC 9 cut(s) 245, 618, 651, 728, 731, 740, 752, 1075, 1084
HpyF3I CTNAG 4 cut(s) 197, 779, 815, 830
HpySE526I ACGT 3 cut(s) 98, 286, 690
Hsp92II CATG 5 cut(s) 376, 380, 528, 703, 887
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 2 cut(s) 664, 1048
KspAI GTTAAC 1 cut(s) 550
Kzo9I GATC 5 cut(s) 121, 664, 986, 1003, 1048
LmnI GCTCC 2 cut(s) 349, 1084
Lsp1109I GCAGC 7 cut(s) 517, 558, 734, 755, 958, 1059, 1087
LweI GCATC 3 cut(s) 409, 948, 1056
MaeI CTAG 4 cut(s) 323, 389, 906, 914
MaeII ACGT 3 cut(s) 98, 286, 690
MaeIII GTNAC 1 cut(s) 691
MalI GATC 5 cut(s) 123, 666, 988, 1005, 1050
MboI GATC 5 cut(s) 121, 664, 986, 1003, 1048
MboII GAAGA 7 cut(s) 5, 59, 181, 325, 434, 624, 1023
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 1 cut(s) 986
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 8 cut(s) 543, 554, 601, 802, 859, 1012, 1018, 1096
MlyI GAGTC 4 cut(s) 19, 216, 527, 1097
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 6 cut(s) 27, 158, 424, 505, 733, 796
Mph1103I ATGCAT 1 cut(s) 378
MroNI GCCGGC 1 cut(s) 248
MseI TTAA 3 cut(s) 126, 549, 594
MspA1I CMGCKG 3 cut(s) 612, 731, 1072
MspI CCGG 1 cut(s) 249
MspR9I CCNGG 2 cut(s) 21, 153
MunI CAATTG 1 cut(s) 543
Mva1269I GAATGC 1 cut(s) 1078
MvaI CCWGG 2 cut(s) 21, 153
MwoI GCNNNNNNNGC 9 cut(s) 245, 618, 651, 728, 731, 740, 752, 1075, 1084
NaeI GCCGGC 1 cut(s) 250
NdeII GATC 5 cut(s) 121, 664, 986, 1003, 1048
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 376, 380, 528, 703, 887
NlaIV GGNNCC 2 cut(s) 146, 988
NsiI ATGCAT 1 cut(s) 378
PcsI WCGNNNNNNNCGW 2 cut(s) 564, 803
PctI GAATGC 1 cut(s) 1078
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 3 cut(s) 30, 790, 1061
PleI GAGTC 4 cut(s) 18, 216, 526, 1097
PpsI GAGTC 4 cut(s) 18, 216, 526, 1097
PpuMI RGGWCCY 1 cut(s) 145
Psp124BI GAGCTC 1 cut(s) 346
Psp5II RGGWCCY 1 cut(s) 145
Psp6I CCWGG 2 cut(s) 19, 151
PspGI CCWGG 2 cut(s) 19, 151
PspN4I GGNNCC 2 cut(s) 146, 988
PspPI GGNCC 1 cut(s) 145
PspPPI RGGWCCY 1 cut(s) 145
PstI CTGCAG 1 cut(s) 1077
PsuI RGATCY 1 cut(s) 986
RsaI GTAC 5 cut(s) 185, 841, 910, 994, 1042
RsaNI GTAC 5 cut(s) 184, 840, 909, 993, 1041
SacI GAGCTC 1 cut(s) 346
SaqAI TTAA 3 cut(s) 126, 549, 594
Sau3AI GATC 5 cut(s) 121, 664, 986, 1003, 1048
Sau96I GGNCC 1 cut(s) 145
ScaI AGTACT 4 cut(s) 841, 910, 994, 1042
SchI GAGTC 4 cut(s) 19, 216, 527, 1097
ScrFI CCNGG 2 cut(s) 21, 153
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 3 cut(s) 409, 948, 1056
SfcI CTRYAG 3 cut(s) 39, 894, 1073
SinI GGWCC 1 cut(s) 145
SpeI ACTAGT 1 cut(s) 905
Sse9I AATT 8 cut(s) 543, 554, 601, 802, 859, 1012, 1018, 1096
SsiI CCGC 4 cut(s) 612, 731, 747, 1070
SspI AATATT 2 cut(s) 592, 658
SspMI CTAG 4 cut(s) 323, 389, 906, 914
SstI GAGCTC 1 cut(s) 346
StyD4I CCNGG 2 cut(s) 19, 151
StyI CCWWGG 2 cut(s) 140, 347
TaiI ACGT 3 cut(s) 101, 289, 693
TaqI TCGA 6 cut(s) 101, 164, 567, 788, 1037, 1064
TaqII GACCGA 1 cut(s) 219
TasI AATT 8 cut(s) 543, 554, 601, 802, 859, 1012, 1018, 1096
TatI WGTACW 4 cut(s) 839, 908, 992, 1040
TauI GCSGC 3 cut(s) 734, 749, 1072
TfiI GAWTC 3 cut(s) 30, 790, 1061
Tru1I TTAA 3 cut(s) 126, 549, 594
Tru9I TTAA 3 cut(s) 126, 549, 594
TscAI CASTG 1 cut(s) 471
TseI GCWGC 7 cut(s) 505, 571, 722, 743, 946, 1072, 1075
TspDTI ATGAA 5 cut(s) 60, 326, 393, 594, 750
TspRI CASTG 1 cut(s) 471
VpaK11BI GGWCC 1 cut(s) 145
XapI RAATTY 5 cut(s) 554, 601, 802, 1012, 1018
XbaI TCTAGA 1 cut(s) 913
XcmI CCANNNNNNNNNTGG 1 cut(s) 997
XspI CTAG 4 cut(s) 323, 389, 906, 914
ZrmI AGTACT 4 cut(s) 841, 910, 994, 1042
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.