MD14G1228000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010835
Physical Location & Seq
Forward (+)
30906536 .. 30907315
780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1228000.v1.1.491

Sequence Viewer

Length: 780 bp
ATGCTCCAACATATCCTCATCATGTGCTCAGAAGCCAGTCCTCCGAGATTATCGTTTTCCCAGGATCTTGGACAACCAGATGTTCTACCAGTTGGTCGGAGAGACACATCACTCTTGGACATGAACTGTGACTTTGAGTTCAGCTTCAGCAACAGCAGCTTAGAGCGTCAATCTTCCTCCGCCGACGAGCTCTTTTCGCACGGTGTGATCCTCCCTATGCAGCCGAAAGATAGGGTTTCGAATAGCGAACTTAAACGTTATGCCTCTCTTCCTCCACTTCCAAGTTCTCCAACTTATGACAATCCAAATCCAAAGAAGGAGATCATGAACACGAAGGAAGACATCGATTCGAGTTCGTATCATTTTGAGCAGAAGCCTCAGTCGAAATCCTTCTGGGGATTCAAGAGAAGCATCAGCCTCAACCAAGAAAATAAGAAGAGCTTGCTTTGTTCATTGCAACTCTTGTCAAGAAGCAACTCAACCGGCTCAGCTCCGAATCCGAAAAAAACAATTTTTAAGGATGTTCATAAACAAAAGCAGATGTCGAAGTCCGCATCATGTTCATCATCTTCTTCATCCTCGTCGTCGAATCCGTATGCAACGCTGCTGCCGAGGCCACCGTCGAGGAAGGGTTACAACGGATCATCTTATTACGGCAACGGTGTTCGAGTTAGTCCTGTGTTAAATGTACCATCTCCTTATATTTCTAAAGGAACTGCAAAGCTCTTTTGTTTGGGTTCTTTTTTACACCCCGGGAAAGATAAAAAGGGCAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.62

Weight (kDa)

9.59

Isoelectric Point (pI)

67.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010835)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G48780 AT1G48780 AT3G18300 AT3G18300
fragaria_vesca FvH4_5g11870
malus_domestica MD06G1217800.v1.1 MD14G1228000.v1.1
prunus_persica Prupe.5G223000_v2.0.a1
pyrus_communis pycom06g19400 pycom14g18940
rosa_chinensis RchiOBHm_Chr7g0185721
rosa_laevigata RLG00000004889
rosa_multiflora Rmu_sc0002952.1_g000001
rosa_roxburghii Rroxscaffold_3G00268680
rosa_rugosa Rorug06G0470800
rosa_samantha Rh7AG071700 Rh7BG076300 Rh7CG076500 Rh7DG076300
rosa_wichuraiana Rw7G006350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 180, 552
AclI AACGTT 1 cut(s) 256
AclWI GGATC 3 cut(s) 72, 202, 649
AcuI CTGAAG 1 cut(s) 130
AdeI CACNNNGTG 1 cut(s) 205
AfaI GTAC 1 cut(s) 690
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 6 cut(s) 144, 159, 190, 441, 491, 724
AluI AGCT 6 cut(s) 144, 159, 190, 441, 491, 724
Alw21I GWGCWC 2 cut(s) 29, 192
Alw26I GTCTC 1 cut(s) 96
AlwI GGATC 3 cut(s) 72, 202, 649
Ama87I CYCGRG 1 cut(s) 752
AoxI GGCC 1 cut(s) 614
ApeKI GCWGC 4 cut(s) 156, 220, 604, 607
Asp700I GAANNNNTTC 1 cut(s) 389
AsuC2I CCSGG 2 cut(s) 753, 754
AsuII TTCGAA 1 cut(s) 239
AvaI CYCGRG 1 cut(s) 752
BanII GRGCYC 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 2 cut(s) 29, 192
BbvI GCAGC 4 cut(s) 168, 232, 591, 594
BccI CCATC 1 cut(s) 700
BceAI ACGGC 1 cut(s) 670
BciT130I CCWGG 1 cut(s) 62
BcnI CCSGG 2 cut(s) 753, 754
BcoDI GTCTC 1 cut(s) 96
BisI GCNGC 4 cut(s) 157, 221, 605, 608
BlpI GCTNAGC 1 cut(s) 487
BlsI GCNGC 4 cut(s) 158, 222, 606, 609
Bme1390I CCNGG 3 cut(s) 62, 753, 754
BmeT110I CYCGRG 1 cut(s) 752
BmrFI CCNGG 3 cut(s) 62, 753, 754
BmsI GCATC 2 cut(s) 420, 563
BpiI GAAGAC 1 cut(s) 345
Bpu1102I GCTNAGC 1 cut(s) 487
Bpu14I TTCGAA 1 cut(s) 239
BpuMI CCSGG 2 cut(s) 753, 754
Bsa29I ATCGAT 1 cut(s) 345
BsaJI CCNNGG 4 cut(s) 60, 611, 751, 752
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 2 cut(s) 36, 89
Bse3DI GCAATG 1 cut(s) 452
BseBI CCWGG 1 cut(s) 62
BseCI ATCGAT 1 cut(s) 345
BseDI CCNNGG 4 cut(s) 60, 611, 751, 752
BseGI GGATG 2 cut(s) 526, 575
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 3 cut(s) 42, 392, 501
BseNI ACTGG 2 cut(s) 36, 89
BseXI GCAGC 4 cut(s) 168, 232, 591, 594
BshFI GGCC 1 cut(s) 616
BshVI ATCGAT 1 cut(s) 345
BsiHKAI GWGCWC 2 cut(s) 29, 192
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 2 cut(s) 483, 753
BsmAI GTCTC 1 cut(s) 96
BsnI GGCC 1 cut(s) 616
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 29, 192
Bsp143I GATC 4 cut(s) 64, 207, 321, 641
Bsp1720I GCTNAGC 1 cut(s) 487
BspACI CCGC 2 cut(s) 180, 552
BspANI GGCC 1 cut(s) 616
BspCNI CTCAG 3 cut(s) 41, 391, 500
BspDI ATCGAT 1 cut(s) 345
BspHI TCATGA 1 cut(s) 324
BspPI GGATC 3 cut(s) 72, 202, 649
BspQI GCTCTTC 1 cut(s) 431
BspT104I TTCGAA 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 2 cut(s) 36, 89
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 4 cut(s) 60, 611, 751, 752
BssMI GATC 4 cut(s) 64, 207, 321, 641
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 4 cut(s) 128, 203, 621, 662
Bst6I CTCTTC 2 cut(s) 273, 431
BstBI TTCGAA 1 cut(s) 239
BstC8I GCNNGC 1 cut(s) 443
BstDEI CTNAG 4 cut(s) 28, 160, 378, 487
BstF5I GGATG 2 cut(s) 526, 575
BstKTI GATC 4 cut(s) 67, 210, 324, 644
BstMAI GTCTC 1 cut(s) 96
BstMBI GATC 4 cut(s) 64, 207, 321, 641
BstMWI GCNNNNNNNGC 3 cut(s) 156, 196, 613
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 3 cut(s) 60, 751, 752
BstV1I GCAGC 4 cut(s) 168, 232, 591, 594
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 68
BstYI RGATCY 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 345
BsuRI GGCC 1 cut(s) 616
BsuTUI ATCGAT 1 cut(s) 345
BtsCI GGATG 2 cut(s) 526, 575
Cac8I GCNNGC 1 cut(s) 443
CciI TCATGA 1 cut(s) 324
Cfr10I RCCGGY 1 cut(s) 482
Cfr9I CCCGGG 1 cut(s) 752
ClaI ATCGAT 1 cut(s) 345
CseI GACGC 1 cut(s) 155
Csp6I GTAC 1 cut(s) 689
CviAII CATG 4 cut(s) 22, 121, 325, 558
CviQI GTAC 1 cut(s) 689
DdeI CTNAG 4 cut(s) 28, 160, 378, 487
DpnI GATC 4 cut(s) 66, 209, 323, 643
DpnII GATC 4 cut(s) 64, 207, 321, 641
DraIII CACNNNGTG 1 cut(s) 205
Eam1104I CTCTTC 2 cut(s) 273, 431
EarI CTCTTC 2 cut(s) 273, 431
EciI GGCGGA 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 190
Eco24I GRGCYC 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 130
Eco88I CYCGRG 1 cut(s) 752
EcoICRI GAGCTC 1 cut(s) 190
EcoRII CCWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 192
FaeI CATG 4 cut(s) 25, 124, 328, 561
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FatI CATG 4 cut(s) 21, 120, 324, 557
Fnu4HI GCNGC 4 cut(s) 157, 221, 605, 608
FokI GGATG 2 cut(s) 533, 562
FriOI GRGCYC 1 cut(s) 192
Fsp4HI GCNGC 4 cut(s) 157, 221, 605, 608
GluI GCNGC 4 cut(s) 157, 221, 605, 608
HaeIII GGCC 1 cut(s) 616
HapII CCGG 2 cut(s) 483, 753
HgaI GACGC 1 cut(s) 155
Hin1II CATG 4 cut(s) 25, 124, 328, 561
HinfI GANTC 4 cut(s) 347, 399, 496, 589
HpaII CCGG 2 cut(s) 483, 753
Hpy188I TCNGA 5 cut(s) 31, 45, 99, 495, 501
Hpy188III TCNNGA 3 cut(s) 325, 403, 468
Hpy99I CGWCG 4 cut(s) 188, 586, 589, 625
HpyAV CCTTC 4 cut(s) 310, 328, 400, 622
HpyCH4III ACNGT 4 cut(s) 128, 203, 621, 662
HpyCH4IV ACGT 1 cut(s) 256
HpyCH4V TGCA 4 cut(s) 220, 457, 599, 719
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 196, 613
HpyF3I CTNAG 4 cut(s) 28, 160, 378, 487
HpySE526I ACGT 1 cut(s) 256
Hsp92II CATG 4 cut(s) 25, 124, 328, 561
Kzo9I GATC 4 cut(s) 64, 207, 321, 641
LguI GCTCTTC 1 cut(s) 431
LmnI GCTCC 2 cut(s) 9, 496
LpnPI CCDG 9 cut(s) 47, 49, 74, 90, 102, 379, 496, 690, 766
Lsp1109I GCAGC 4 cut(s) 168, 232, 591, 594
LweI GCATC 2 cut(s) 420, 563
MaeII ACGT 1 cut(s) 256
MaeIII GTNAC 2 cut(s) 128, 632
MalI GATC 4 cut(s) 66, 209, 323, 643
MboI GATC 4 cut(s) 64, 207, 321, 641
MboII GAAGA 6 cut(s) 165, 260, 350, 448, 561, 564
MflI RGATCY 1 cut(s) 64
MhlI GDGCHC 2 cut(s) 29, 192
MluCI AATT 1 cut(s) 510
MmeI TCCRAC 3 cut(s) 31, 77, 314
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 3 cut(s) 252, 516, 683
MspI CCGG 2 cut(s) 483, 753
MspR9I CCNGG 3 cut(s) 62, 753, 754
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 3 cut(s) 156, 196, 613
NciI CCSGG 2 cut(s) 753, 754
NdeII GATC 4 cut(s) 64, 207, 321, 641
NlaIII CATG 4 cut(s) 25, 124, 328, 561
NmeAIII GCCGAG 1 cut(s) 636
NmuCI GTSAC 1 cut(s) 128
NspV TTCGAA 1 cut(s) 239
PagI TCATGA 1 cut(s) 324
PciSI GCTCTTC 1 cut(s) 431
PcsI WCGNNNNNNNCGW 2 cut(s) 590, 608
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 4 cut(s) 347, 399, 496, 589
PkrI GCNGC 4 cut(s) 158, 222, 606, 609
Psp124BI GAGCTC 1 cut(s) 192
Psp1406I AACGTT 1 cut(s) 256
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PsuI RGATCY 1 cut(s) 64
RsaI GTAC 1 cut(s) 690
RsaNI GTAC 1 cut(s) 689
SacI GAGCTC 1 cut(s) 192
SapI GCTCTTC 1 cut(s) 431
SaqAI TTAA 3 cut(s) 252, 516, 683
SatI GCNGC 4 cut(s) 157, 221, 605, 608
Sau3AI GATC 4 cut(s) 64, 207, 321, 641
ScrFI CCNGG 3 cut(s) 62, 753, 754
SduI GDGCHC 2 cut(s) 29, 192
SetI ASST 7 cut(s) 146, 161, 192, 259, 443, 493, 726
SfaNI GCATC 2 cut(s) 420, 563
SfuI TTCGAA 1 cut(s) 239
SmaI CCCGGG 1 cut(s) 754
Sse9I AATT 1 cut(s) 510
SsiI CCGC 2 cut(s) 180, 552
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 3 cut(s) 60, 751, 752
TaaI ACNGT 4 cut(s) 128, 203, 621, 662
TaiI ACGT 1 cut(s) 259
TaqI TCGA 8 cut(s) 239, 345, 350, 383, 545, 587, 623, 667
TasI AATT 1 cut(s) 510
TfiI GAWTC 4 cut(s) 347, 399, 496, 589
Tru1I TTAA 3 cut(s) 252, 516, 683
Tru9I TTAA 3 cut(s) 252, 516, 683
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 4 cut(s) 156, 220, 604, 607
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 6 cut(s) 137, 341, 441, 515, 552, 564
TspGWI ACGGA 2 cut(s) 582, 654
TspMI CCCGGG 1 cut(s) 752
XmaI CCCGGG 1 cut(s) 752
XmnI GAANNNNTTC 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.