MD14G1244400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0017160
Physical Location & Seq
Forward (+)
32264967 .. 32266867
1901 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1244400.v1.1.491

Sequence Viewer

Length: 378 bp
ATGTCGGATCCATTCGAGAGAGCGAAAGGAGGCAGATTGACCTTCAAAGACGGAACTCTCGCCACTCGCAGCAAAGCCATCGACAAGAAGAAGAAGAAAAAGAAGAAGCAGCAGCTTCAGGAGCAGCATATGAACCCTAACGACGATTCCCAAGTGGTGGCTGAGGACGGAAGCGCCGAAGCAGCAGCAGCTGTAGAAGGAACTGGAGGAGGAACGGAGACTTACACCATCGACGCAGCGAAGCGGATGAAGTACGAGCAGCTGTTCCCGGTTGAGGCCAAGAAGTTCGGCTACGATCCCAAATCCGTTGAACGGAAGACCGTCGAGGACGTTCTTGACGACCGCGTCAAGAAGAAAGCCGACCGCTACTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1754

Protein Analysis

126

Amino Acids

14.0

Weight (kDa)

9.32

Isoelectric Point (pI)

37.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017160)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25720
fragaria_vesca FvH4_5g18200
malus_domestica MD14G1244400.v1.1
prunus_persica Prupe.5G241900_v2.0.a1
pyrus_communis pycom14g20550
rosa_chinensis RchiOBHm_Chr7g0177391
rosa_laevigata RLG00000005540
rosa_multiflora Rmu_sc0005545.1_g000002
rosa_rugosa Rorug06G0405500
rosa_samantha Rh7AG005200 Rh7BG005200 Rh7CG005300
rosa_wichuraiana Rw7G000430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AccB7I CCANNNNNTGG 1 cut(s) 157
AccII CGCG 1 cut(s) 345
AciI CCGC 3 cut(s) 244, 343, 364
AclWI GGATC 3 cut(s) 2, 15, 290
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 3 cut(s) 157, 274, 312
AgsI TTSAA 2 cut(s) 46, 311
AluBI AGCT 3 cut(s) 115, 191, 262
AluI AGCT 3 cut(s) 115, 191, 262
Alw26I GTCTC 1 cut(s) 212
AlwI GGATC 3 cut(s) 2, 15, 290
AlwNI CAGNNNCTG 1 cut(s) 191
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 9 cut(s) 69, 109, 112, 124, 182, 185, 188, 236, 259
AspLEI GCGC 1 cut(s) 176
AsuC2I CCSGG 1 cut(s) 269
BamHI GGATCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BbsI GAAGAC 1 cut(s) 323
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 9 cut(s) 81, 121, 124, 136, 194, 197, 200, 248, 271
BccI CCATC 2 cut(s) 86, 236
BcgI CGANNNNNNTGC 2 cut(s) 61, 95
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 212
BfmI CTRYAG 1 cut(s) 192
BfoI RGCGCY 1 cut(s) 177
BisI GCNGC 9 cut(s) 70, 110, 113, 125, 183, 186, 189, 237, 260
BlsI GCNGC 9 cut(s) 71, 111, 114, 126, 184, 187, 190, 238, 261
Bme1390I CCNGG 1 cut(s) 269
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 269
BpiI GAAGAC 1 cut(s) 323
BpmI CTGGAG 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 162
BpuMI CCSGG 1 cut(s) 269
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 274, 312
Bse1I ACTGG 1 cut(s) 208
BseGI GGATG 1 cut(s) 252
BseLI CCNNNNNNNGG 3 cut(s) 157, 274, 312
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 222
BseXI GCAGC 9 cut(s) 81, 121, 124, 136, 194, 197, 200, 248, 271
Bsh1236I CGCG 1 cut(s) 345
Bsh1285I CGRYCG 2 cut(s) 343, 364
BshFI GGCC 1 cut(s) 278
BsiEI CGRYCG 2 cut(s) 343, 364
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 157, 274, 312
BsmAI GTCTC 1 cut(s) 212
BsnI GGCC 1 cut(s) 278
Bsp143I GATC 2 cut(s) 7, 295
BspACI CCGC 3 cut(s) 244, 343, 364
BspANI GGCC 1 cut(s) 278
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 345
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 3 cut(s) 2, 15, 290
BsrI ACTGG 1 cut(s) 208
BssMI GATC 2 cut(s) 7, 295
Bst4CI ACNGT 2 cut(s) 322, 371
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 1 cut(s) 252
BstFNI CGCG 1 cut(s) 345
BstH2I RGCGCY 1 cut(s) 177
BstHHI GCGC 1 cut(s) 176
BstKTI GATC 2 cut(s) 10, 298
BstMAI GTCTC 1 cut(s) 212
BstMBI GATC 2 cut(s) 7, 295
BstMCI CGRYCG 2 cut(s) 343, 364
BstMWI GCNNNNNNNGC 3 cut(s) 121, 182, 188
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 1 cut(s) 345
BstV1I GCAGC 9 cut(s) 81, 121, 124, 136, 194, 197, 200, 248, 271
BstV2I GAAGAC 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 252
CaiI CAGNNNCTG 1 cut(s) 191
CfoI GCGC 1 cut(s) 176
CseI GACGC 2 cut(s) 242, 334
Csp6I GTAC 1 cut(s) 253
CviJI RGCY 8 cut(s) 77, 115, 161, 191, 262, 278, 291, 359
CviKI_1 RGCY 8 cut(s) 77, 115, 161, 191, 262, 278, 291, 359
CviQI GTAC 1 cut(s) 253
DdeI CTNAG 1 cut(s) 162
DpnI GATC 2 cut(s) 9, 297
DpnII GATC 2 cut(s) 7, 295
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 101
FaiI YATR 2 cut(s) 129, 131
FauNDI CATATG 1 cut(s) 129
Fnu4HI GCNGC 9 cut(s) 70, 110, 113, 125, 183, 186, 189, 237, 260
FokI GGATG 1 cut(s) 259
Fsp4HI GCNGC 9 cut(s) 70, 110, 113, 125, 183, 186, 189, 237, 260
GlaI GCGC 1 cut(s) 175
GluI GCNGC 9 cut(s) 70, 110, 113, 125, 183, 186, 189, 237, 260
GsuI CTGGAG 1 cut(s) 225
HaeII RGCGCY 1 cut(s) 177
HaeIII GGCC 1 cut(s) 278
HapII CCGG 1 cut(s) 269
HgaI GACGC 2 cut(s) 242, 334
HhaI GCGC 1 cut(s) 176
Hin6I GCGC 1 cut(s) 174
HinP1I GCGC 1 cut(s) 174
HinfI GANTC 1 cut(s) 146
HpaII CCGG 1 cut(s) 269
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 4 cut(s) 16, 119, 335, 349
Hpy99I CGWCG 3 cut(s) 146, 236, 326
HpyAV CCTTC 2 cut(s) 52, 191
HpyCH4III ACNGT 2 cut(s) 322, 371
HpyCH4IV ACGT 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 182, 188
HpyF3I CTNAG 1 cut(s) 162
HpySE526I ACGT 1 cut(s) 330
HspAI GCGC 1 cut(s) 174
Kzo9I GATC 2 cut(s) 7, 295
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 3 cut(s) 104, 189, 282
Lsp1109I GCAGC 9 cut(s) 81, 121, 124, 136, 194, 197, 200, 248, 271
MaeII ACGT 1 cut(s) 330
MalI GATC 2 cut(s) 9, 297
MboI GATC 2 cut(s) 7, 295
MboII GAAGA 6 cut(s) 100, 103, 106, 115, 328, 364
MflI RGATCY 1 cut(s) 7
MnlI CCTC 6 cut(s) 23, 157, 200, 203, 268, 319
MspA1I CMGCKG 2 cut(s) 191, 262
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 121, 182, 188
NciI CCSGG 1 cut(s) 269
NdeI CATATG 1 cut(s) 129
NdeII GATC 2 cut(s) 7, 295
NlaIV GGNNCC 1 cut(s) 9
PcsI WCGNNNNNNNCGW 2 cut(s) 174, 336
PfeI GAWTC 1 cut(s) 146
PflFI GACNNNGTC 1 cut(s) 344
PflMI CCANNNNNTGG 1 cut(s) 157
PkrI GCNGC 9 cut(s) 71, 111, 114, 126, 184, 187, 190, 238, 261
PspN4I GGNNCC 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 191
PsuI RGATCY 1 cut(s) 7
PsyI GACNNNGTC 1 cut(s) 344
PvuII CAGCTG 2 cut(s) 191, 262
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SatI GCNGC 9 cut(s) 70, 110, 113, 125, 183, 186, 189, 237, 260
Sau3AI GATC 2 cut(s) 7, 295
ScrFI CCNGG 1 cut(s) 269
SetI ASST 5 cut(s) 44, 117, 193, 264, 333
SfcI CTRYAG 1 cut(s) 192
SsiI CCGC 3 cut(s) 244, 343, 364
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 322, 371
TaiI ACGT 1 cut(s) 333
TaqI TCGA 4 cut(s) 15, 81, 231, 324
TfiI GAWTC 1 cut(s) 146
TseI GCWGC 9 cut(s) 69, 109, 112, 124, 182, 185, 188, 236, 259
TspDTI ATGAA 2 cut(s) 146, 263
TspGWI ACGGA 5 cut(s) 66, 183, 230, 295, 328
Tth111I GACNNNGTC 1 cut(s) 344
Van91I CCANNNNNTGG 1 cut(s) 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.