MD14G1245300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009074
Physical Location & Seq
Reverse (-)
32337666 .. 32341748
4083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1245300.v1.1.491

Sequence Viewer

Length: 1167 bp
ATGTCGTCCTCGGGCGGCCACTCCGGCAGTGTTGGCGGTCCGATCGCCCCTCAGCTTTACTTCTGCCACCGGTGCGACCGTACGGTTTCCCTCACCCCCTCACCTACCTACGATCTCGTTTGCCCTCTTTGCAGCGGCGGCTTCCTCGAAGAGATGGAAACCCCCAACCCTAACCCTAACCCTTTCCCCCCCAATCCTTTCTTCTCCTTCCCCTCCTCCGACGCCCCTCCCTTCGCCTCCAGCGGATTTCCCCTCCTCTTCTCCTCCTCCGCCTCTGGTCCCGCTCCCGATTCCGCTCCCGCCCCTGCCAATCTCGCCGATGATCTCTCCTCCCTCTTCGGTGGCGCACCCTCACGCTCCTCACCCTTTCAGGACCCCGACGCCTTCAACCCCTTCGTCTTCCTCCAGAACTACTTGCAAACCCTGAGGGCAAATGGTGCCAATGTCCAGTTCGTGATCGATGGCAGCCCTGGCGGCGGCGGCGGGGACCCATTTGGTTTTCGTGTTCCCACCAATCTTAATCTCGGCGATTACTTCTTTGGCCCGGGCATCGAGCAATTGATCCAACAATTAGCCGAGAACGATCCAAACAGGTACGGCACCCCACCTGCCTCTAAGTCCGCCATCGTAGCCTTGCCGGTTGTGAAAATCTCAGAGGAGTTGTTGGCTTCGGATTCGTCACAGTGCGCGGTGTGTAAGGACTCATTCGAGCTCGGTGAGGAGGCCAAACAGATGCCCTGTAAGCACTTATACCACTCGGATTGTATTCTGCCTTGGCTGGAATTGCATAACTCATGTCCTGTCTGTCGCCACGAGTTGCCCACTGATGATCCTGAATACGAGCAGAGGATCCGTGGGGACTCCGGAAATGGCAATCAAACTAGTCAATCTGCGGCTCCTGGTGGGACTAATATTTCTATGGGCGGTGGTGGGAGTGGTAGTGGGTTTGGGTTAGGAATTGGCACGCCATCTCTAGACAATCCGAGTCCAAGTCCTCGAACGGTGGAGCGGAGGTTTACCATTTCCCTACCACGCTTTTTTGGGGCACTTGGTGGTCCGGCCGAGACTAGCAATACTGGGAGTGGAAACAATGAGGAGTCGAACTCAGGGAATAGGGGAAGGCATAACCCTGGATCGGAGGAGCCTAGGCAAGAAGATCTTGATTGA

Protein Analysis

389

Amino Acids

40.73

Weight (kDa)

4.58

Isoelectric Point (pI)

65.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 18 - 52 8.9e-14 zinc-ribbon
zf-RING_2 PF13639 228 - 270 5e-13 Ring finger domain
zf-C3HC4_2 PF13923 228 - 269 7.8e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 228 - 256 7.6e-08 RING-like zinc finger
zf-C3HC4 PF00097 229 - 269 2.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 616
Acc36I ACCTGC 1 cut(s) 616
AccB1I GGYRCC 2 cut(s) 437, 599
AccBSI CCGCTC 3 cut(s) 284, 296, 1009
AccII CGCG 1 cut(s) 689
AccIII TCCGGA 1 cut(s) 863
AclWI GGATC 6 cut(s) 556, 578, 824, 844, 857, 1141
AcoI YGGCCR 2 cut(s) 16, 1059
AcyI GRCGYC 2 cut(s) 222, 381
AdeI CACNNNGTG 1 cut(s) 1052
AfaI GTAC 2 cut(s) 82, 596
AfiI CCNNNNNNNGG 2 cut(s) 476, 1135
AgeI ACCGGT 1 cut(s) 69
AgsI TTSAA 1 cut(s) 388
AhlI ACTAGT 1 cut(s) 881
AjnI CCWGG 3 cut(s) 469, 898, 1129
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 434, 466
AluBI AGCT 2 cut(s) 55, 712
AluI AGCT 2 cut(s) 55, 712
Alw21I GWGCWC 1 cut(s) 714
Alw26I GTCTC 1 cut(s) 1058
AlwI GGATC 6 cut(s) 556, 578, 824, 844, 857, 1141
Ama87I CYCGRG 2 cut(s) 10, 544
Aor13HI TCCGGA 1 cut(s) 863
AoxI GGCC 4 cut(s) 16, 541, 723, 1059
ApeKI GCWGC 2 cut(s) 132, 465
AsiGI ACCGGT 1 cut(s) 69
AspA2I CCTAGG 1 cut(s) 1145
AspLEI GCGC 2 cut(s) 347, 689
AspS9I GGNCC 6 cut(s) 38, 278, 373, 487, 542, 1055
AsuC2I CCSGG 2 cut(s) 545, 546
AsuHPI GGTGA 4 cut(s) 85, 93, 354, 728
AvaI CYCGRG 2 cut(s) 10, 544
AvaII GGWCC 5 cut(s) 38, 278, 373, 487, 1055
AvrII CCTAGG 1 cut(s) 1145
AxyI CCTNAGG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 1048
BamHI GGATCC 1 cut(s) 849
BanI GGYRCC 2 cut(s) 437, 599
BanII GRGCYC 1 cut(s) 714
BauI CACGAG 1 cut(s) 812
BbsI GAAGAC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 714
BbvCI CCTCAGC 1 cut(s) 51
BbvI GCAGC 2 cut(s) 144, 477
BccI CCATC 4 cut(s) 148, 455, 632, 976
BceAI ACGGC 1 cut(s) 613
BciT130I CCWGG 3 cut(s) 471, 900, 1131
BcnI CCSGG 2 cut(s) 545, 546
BcoDI GTCTC 1 cut(s) 1058
BcuI ACTAGT 1 cut(s) 881
BfaI CTAG 4 cut(s) 882, 974, 1068, 1146
BfuAI ACCTGC 1 cut(s) 616
BglI GCCNNNNNGGC 2 cut(s) 24, 474
BglII AGATCT 1 cut(s) 1156
BisI GCNGC 9 cut(s) 16, 133, 136, 139, 466, 475, 478, 481, 894
BlnI CCTAGG 1 cut(s) 1145
BlsI GCNGC 9 cut(s) 17, 134, 137, 140, 467, 476, 479, 482, 895
Bme1390I CCNGG 5 cut(s) 471, 545, 546, 900, 1131
Bme18I GGWCC 5 cut(s) 38, 278, 373, 487, 1055
BmeT110I CYCGRG 2 cut(s) 10, 544
BmgT120I GGNCC 6 cut(s) 38, 278, 373, 487, 542, 1055
BmiI GGNNCC 9 cut(s) 280, 375, 439, 488, 489, 601, 851, 897, 1143
BmrFI CCNGG 5 cut(s) 471, 545, 546, 900, 1131
BmrI ACTGGG 1 cut(s) 1086
BmsI GCATC 2 cut(s) 558, 723
BmuI ACTGGG 1 cut(s) 1086
BpiI GAAGAC 1 cut(s) 391
BplI GAGNNNNNCTC 2 cut(s) 1088, 1120
BpmI CTGGAG 2 cut(s) 223, 389
Bpu10I CCTNAGC 1 cut(s) 51
BpuMI CCSGG 2 cut(s) 545, 546
Bsa29I ATCGAT 1 cut(s) 459
BsaHI GRCGYC 2 cut(s) 222, 381
BsaJI CCNNGG 7 cut(s) 9, 469, 544, 773, 853, 1129, 1145
BsaWI WCCGGW 2 cut(s) 69, 863
BsaXI ACNNNNNCTCC 4 cut(s) 925, 955, 998, 1028
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 1135
Bse118I RCCGGY 2 cut(s) 69, 637
Bse1I ACTGG 2 cut(s) 448, 1081
Bse21I CCTNAGG 1 cut(s) 425
BseAI TCCGGA 1 cut(s) 863
BseBI CCWGG 3 cut(s) 471, 900, 1131
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 7 cut(s) 9, 469, 544, 773, 853, 1129, 1145
BseLI CCNNNNNNNGG 2 cut(s) 476, 1135
BseMII CTCAG 4 cut(s) 65, 416, 666, 1119
BseNI ACTGG 2 cut(s) 448, 1081
BseSI GKGCMC 1 cut(s) 1048
BseX3I CGGCCG 1 cut(s) 1059
BseXI GCAGC 2 cut(s) 144, 477
Bsh1236I CGCG 1 cut(s) 689
Bsh1285I CGRYCG 3 cut(s) 45, 79, 1062
BshFI GGCC 4 cut(s) 18, 543, 725, 1061
BshNI GGYRCC 2 cut(s) 437, 599
BshTI ACCGGT 1 cut(s) 69
BshVI ATCGAT 1 cut(s) 459
BsiEI CGRYCG 3 cut(s) 45, 79, 1062
BsiHKAI GWGCWC 1 cut(s) 714
BsiHKCI CYCGRG 2 cut(s) 10, 544
BsiSI CCGG 6 cut(s) 24, 70, 545, 638, 864, 1058
BsiWI CGTACG 1 cut(s) 80
BslFI GGGAC 4 cut(s) 264, 500, 872, 919
BslI CCNNNNNNNGG 2 cut(s) 476, 1135
BsmAI GTCTC 1 cut(s) 1058
BsmFI GGGAC 4 cut(s) 264, 500, 872, 919
BsnI GGCC 4 cut(s) 18, 543, 725, 1061
BsoBI CYCGRG 2 cut(s) 10, 544
Bsp1286I GDGCHC 2 cut(s) 714, 1048
Bsp13I TCCGGA 1 cut(s) 863
BspANI GGCC 4 cut(s) 18, 543, 725, 1061
BspCNI CTCAG 4 cut(s) 64, 417, 665, 1118
BspDI ATCGAT 1 cut(s) 459
BspEI TCCGGA 1 cut(s) 863
BspFNI CGCG 1 cut(s) 689
BspLI GGNNCC 9 cut(s) 280, 375, 439, 488, 489, 601, 851, 897, 1143
BspMI ACCTGC 1 cut(s) 616
BspPI GGATC 6 cut(s) 556, 578, 824, 844, 857, 1141
BspT107I GGYRCC 2 cut(s) 437, 599
BsrBI CCGCTC 3 cut(s) 284, 296, 1009
BsrFI RCCGGY 2 cut(s) 69, 637
BsrI ACTGG 2 cut(s) 448, 1081
BssAI RCCGGY 2 cut(s) 69, 637
BssECI CCNNGG 7 cut(s) 9, 469, 544, 773, 853, 1129, 1145
BssNI GRCGYC 2 cut(s) 222, 381
BssSI CACGAG 1 cut(s) 812
BssT1I CCWWGG 2 cut(s) 773, 1145
Bst2BI CACGAG 1 cut(s) 812
Bst2UI CCWGG 3 cut(s) 471, 900, 1131
Bst4CI ACNGT 4 cut(s) 80, 85, 684, 1003
Bst6I CTCTTC 3 cut(s) 144, 263, 341
BstACI GRCGYC 2 cut(s) 222, 381
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 1 cut(s) 965
BstDEI CTNAG 5 cut(s) 51, 425, 615, 652, 1105
BstDSI CCRYGG 1 cut(s) 853
BstFNI CGCG 1 cut(s) 689
BstHHI GCGC 2 cut(s) 347, 689
BstMAI GTCTC 1 cut(s) 1058
BstMCI CGRYCG 3 cut(s) 45, 79, 1062
BstNI CCWGG 3 cut(s) 471, 900, 1131
BstSCI CCNGG 5 cut(s) 469, 543, 544, 898, 1129
BstSLI GKGCMC 1 cut(s) 1048
BstUI CGCG 1 cut(s) 689
BstV1I GCAGC 2 cut(s) 144, 477
BstV2I GAAGAC 1 cut(s) 391
BstX2I RGATCY 2 cut(s) 849, 1156
BstYI RGATCY 2 cut(s) 849, 1156
BstZI CGGCCG 1 cut(s) 1059
Bsu15I ATCGAT 1 cut(s) 459
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 4 cut(s) 18, 543, 725, 1061
BsuTUI ATCGAT 1 cut(s) 459
BtgI CCRYGG 1 cut(s) 853
BtsI GCAGTG 1 cut(s) 34
BtsIMutI CAGTG 3 cut(s) 34, 689, 822
BveI ACCTGC 1 cut(s) 616
Cac8I GCNNGC 1 cut(s) 965
CfoI GCGC 2 cut(s) 347, 689
Cfr10I RCCGGY 2 cut(s) 69, 637
Cfr13I GGNCC 6 cut(s) 38, 278, 373, 487, 542, 1055
Cfr9I CCCGGG 1 cut(s) 544
ClaI ATCGAT 1 cut(s) 459
CpoI CGGWCCG 1 cut(s) 38
CseI GACGC 2 cut(s) 230, 389
Csp6I GTAC 2 cut(s) 81, 595
CspAI ACCGGT 1 cut(s) 69
CspCI CAANNNNNGTGG 2 cut(s) 743, 778
CspI CGGWCCG 1 cut(s) 38
CviAII CATG 1 cut(s) 795
CviQI GTAC 2 cut(s) 81, 595
DdeI CTNAG 5 cut(s) 51, 425, 615, 652, 1105
DraIII CACNNNGTG 1 cut(s) 1052
EaeI YGGCCR 2 cut(s) 16, 1059
EagI CGGCCG 1 cut(s) 1059
Eam1104I CTCTTC 3 cut(s) 144, 263, 341
EarI CTCTTC 3 cut(s) 144, 263, 341
EciI GGCGGA 2 cut(s) 259, 610
Ecl136II GAGCTC 1 cut(s) 712
EclXI CGGCCG 1 cut(s) 1059
Eco130I CCWWGG 2 cut(s) 773, 1145
Eco24I GRGCYC 1 cut(s) 714
Eco47I GGWCC 5 cut(s) 38, 278, 373, 487, 1055
Eco52I CGGCCG 1 cut(s) 1059
Eco53kI GAGCTC 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 425
Eco88I CYCGRG 2 cut(s) 10, 544
EcoICRI GAGCTC 1 cut(s) 712
EcoO109I RGGNCCY 2 cut(s) 373, 487
EcoRII CCWGG 3 cut(s) 469, 898, 1129
EcoT14I CCWWGG 2 cut(s) 773, 1145
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 2 cut(s) 773, 1145
FaeI CATG 1 cut(s) 798
FaiI YATR 5 cut(s) 751, 789, 796, 920, 1125
FalI AAGNNNNNCTT 1 cut(s) 1143
FaqI GGGAC 4 cut(s) 264, 500, 872, 919
FatI CATG 1 cut(s) 794
FauI CCCGC 3 cut(s) 289, 307, 476
Fnu4HI GCNGC 9 cut(s) 16, 133, 136, 139, 466, 475, 478, 481, 894
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 9 cut(s) 16, 133, 136, 139, 466, 475, 478, 481, 894
FspBI CTAG 4 cut(s) 882, 974, 1068, 1146
GlaI GCGC 2 cut(s) 346, 688
GluI GCNGC 9 cut(s) 16, 133, 136, 139, 466, 475, 478, 481, 894
GsuI CTGGAG 2 cut(s) 223, 389
HaeIII GGCC 4 cut(s) 18, 543, 725, 1061
HapII CCGG 6 cut(s) 24, 70, 545, 638, 864, 1058
HgaI GACGC 2 cut(s) 230, 389
HhaI GCGC 2 cut(s) 347, 689
Hin1I GRCGYC 2 cut(s) 222, 381
Hin1II CATG 1 cut(s) 798
Hin6I GCGC 2 cut(s) 345, 687
HinP1I GCGC 2 cut(s) 345, 687
HinfI GANTC 6 cut(s) 290, 674, 701, 860, 985, 1097
HpaII CCGG 6 cut(s) 24, 70, 545, 638, 864, 1058
HphI GGTGA 4 cut(s) 85, 93, 354, 728
Hpy166II GTNNAC 1 cut(s) 1017
Hpy188I TCNGA 7 cut(s) 42, 220, 655, 673, 760, 984, 1138
Hpy188III TCNNGA 8 cut(s) 287, 371, 406, 454, 833, 864, 974, 1160
Hpy8I GTNNAC 1 cut(s) 1017
Hpy99I CGWCG 2 cut(s) 224, 383
HpyAV CCTTC 5 cut(s) 217, 241, 394, 403, 1113
HpyCH4III ACNGT 4 cut(s) 80, 85, 684, 1003
HpyCH4V TGCA 3 cut(s) 132, 418, 787
HpyF3I CTNAG 5 cut(s) 51, 425, 615, 652, 1105
Hsp92I GRCGYC 2 cut(s) 222, 381
Hsp92II CATG 1 cut(s) 798
HspAI GCGC 2 cut(s) 345, 687
KflI GGGWCCC 1 cut(s) 487
Kpn2I TCCGGA 1 cut(s) 863
LmnI GCTCC 6 cut(s) 289, 301, 362, 901, 1006, 1141
Lsp1109I GCAGC 2 cut(s) 144, 477
LweI GCATC 2 cut(s) 558, 723
MaeI CTAG 4 cut(s) 882, 974, 1068, 1146
MaeIII GTNAC 1 cut(s) 678
MbiI CCGCTC 3 cut(s) 284, 296, 1009
MboII GAAGA 6 cut(s) 161, 193, 250, 328, 391, 1166
MfeI CAATTG 1 cut(s) 557
MflI RGATCY 2 cut(s) 849, 1156
MhlI GDGCHC 2 cut(s) 714, 1048
MluCI AATT 4 cut(s) 557, 569, 782, 957
MlyI GAGTC 4 cut(s) 695, 854, 994, 1106
MmeI TCCRAC 2 cut(s) 243, 589
MroI TCCGGA 1 cut(s) 863
MseI TTAA 1 cut(s) 519
MspA1I CMGCKG 2 cut(s) 135, 243
MspI CCGG 6 cut(s) 24, 70, 545, 638, 864, 1058
MspR9I CCNGG 5 cut(s) 471, 545, 546, 900, 1131
MunI CAATTG 1 cut(s) 557
MvaI CCWGG 3 cut(s) 471, 900, 1131
MvnI CGCG 1 cut(s) 689
NciI CCSGG 2 cut(s) 545, 546
NlaIII CATG 1 cut(s) 798
NlaIV GGNNCC 9 cut(s) 280, 375, 439, 488, 489, 601, 851, 897, 1143
NmeAIII GCCGAG 3 cut(s) 504, 601, 1087
NmuCI GTSAC 1 cut(s) 678
PaqCI CACCTGC 1 cut(s) 616
PfeI GAWTC 2 cut(s) 290, 674
Pfl23II CGTACG 1 cut(s) 80
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 9 cut(s) 17, 134, 137, 140, 467, 476, 479, 482, 895
Ple19I CGATCG 1 cut(s) 45
PleI GAGTC 4 cut(s) 695, 854, 993, 1105
PpsI GAGTC 4 cut(s) 695, 854, 993, 1105
PpuMI RGGWCCY 2 cut(s) 373, 487
Psp124BI GAGCTC 1 cut(s) 714
Psp5II RGGWCCY 2 cut(s) 373, 487
Psp6I CCWGG 3 cut(s) 469, 898, 1129
PspGI CCWGG 3 cut(s) 469, 898, 1129
PspLI CGTACG 1 cut(s) 80
PspN4I GGNNCC 9 cut(s) 280, 375, 439, 488, 489, 601, 851, 897, 1143
PspPI GGNCC 6 cut(s) 38, 278, 373, 487, 542, 1055
PspPPI RGGWCCY 2 cut(s) 373, 487
PsuI RGATCY 2 cut(s) 849, 1156
PvuI CGATCG 1 cut(s) 45
RsaI GTAC 2 cut(s) 82, 596
RsaNI GTAC 2 cut(s) 81, 595
Rsr2I CGGWCCG 1 cut(s) 38
RsrII CGGWCCG 1 cut(s) 38
SacI GAGCTC 1 cut(s) 714
SaqAI TTAA 1 cut(s) 519
SatI GCNGC 9 cut(s) 16, 133, 136, 139, 466, 475, 478, 481, 894
Sau96I GGNCC 6 cut(s) 38, 278, 373, 487, 542, 1055
SchI GAGTC 4 cut(s) 695, 854, 994, 1106
ScrFI CCNGG 5 cut(s) 471, 545, 546, 900, 1131
SduI GDGCHC 2 cut(s) 714, 1048
SetI ASST 7 cut(s) 57, 106, 110, 596, 610, 714, 1016
SfaNI GCATC 2 cut(s) 558, 723
SgrAI CRCCGGYG 1 cut(s) 69
SinI GGWCC 5 cut(s) 38, 278, 373, 487, 1055
SmaI CCCGGG 1 cut(s) 546
SpeI ACTAGT 1 cut(s) 881
SrfI GCCCGGGC 1 cut(s) 546
Sse9I AATT 4 cut(s) 557, 569, 782, 957
SspI AATATT 1 cut(s) 913
SspMI CTAG 4 cut(s) 882, 974, 1068, 1146
SstI GAGCTC 1 cut(s) 714
StyD4I CCNGG 5 cut(s) 469, 543, 544, 898, 1129
StyI CCWWGG 2 cut(s) 773, 1145
TaaI ACNGT 4 cut(s) 80, 85, 684, 1003
TaqI TCGA 6 cut(s) 147, 459, 552, 708, 997, 1100
TasI AATT 4 cut(s) 557, 569, 782, 957
TauI GCSGC 7 cut(s) 18, 138, 141, 477, 480, 483, 896
TfiI GAWTC 2 cut(s) 290, 674
Tru1I TTAA 1 cut(s) 519
Tru9I TTAA 1 cut(s) 519
TscAI CASTG 3 cut(s) 34, 689, 829
TseFI GTSAC 1 cut(s) 678
TseI GCWGC 2 cut(s) 132, 465
Tsp45I GTSAC 1 cut(s) 678
TspGWI ACGGA 1 cut(s) 842
TspMI CCCGGG 1 cut(s) 544
TspRI CASTG 3 cut(s) 34, 689, 829
VpaK11BI GGWCC 5 cut(s) 38, 278, 373, 487, 1055
XbaI TCTAGA 1 cut(s) 973
XmaI CCCGGG 1 cut(s) 544
XmaJI CCTAGG 1 cut(s) 1145
XspI CTAG 4 cut(s) 882, 974, 1068, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.