Rh7DG003800

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0009074
Physical Location & Seq
Reverse (-)
336578 .. 341607
5030 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG003800.1

Sequence Viewer

Length: 1122 bp
ATGTCGTCCTCGGGCGGCAACTCCGGCATCGCCCCCGCTCAGCAGTACTTTTGCCACCAGTGCGATCGCACCGTTTCCCTCAACCCTTCCCCTACCTCCGATCTCAATTGCCCTATTTGCAGTGGCGGCTTCCTCGAAGAGATGGAAACCTCTAACCCTAACCCTTCCCCCAATTCCTTCTTCTCCTTCCCCTCCGACGCCCCTCCCTTCTCCTCCTCCTCCAGCGGCTTTCCCTCCTTTCTCTTCAGCCCCGCCGCCGGTGGTCCGGGAAATCTTGGCGACGAACTCTCCGCCCTCTTCGGTGGTGGGGCGCCTTCCTCCCGCTCGTCCCCCTATCAGGACCCCGACGCCTTCGACCCCTTTGTGTTCCTCCAGAACTATTTGCAAACCCTAAGGGCCAATGGTGCCAATGTTCAGTTTGTGATTGATAACAGCGGCGGAGGAGGAGGAGGAGGAGGAGTAGGGGATCCCTCAGCCTTCCGTGTTCCCACCAATCTTAATTTTGGAGATTACTTCTTCGGCCCAGGCCTCGAGCAACTCATCCAGCAGCTTGCCGAGAATGATCCAAACCGCTACGGCACGCCACCGGCTTCAAAGTCTGCCATCGAGGCCTTGCCAGTTGTGAAAATCTCAAAGGAACTCTTGGATTCTGATTCTTCTCAGTGCGCAGTGTGTAAGGACTCGTTTGAGCTTGGGGAGGAGGCCAAGCAGATGCCCTGCAAGCACTTATACCATTCTGATTGTATTCTTCCCTGGTTGGAATTGCATAACTCATGCCCTGTTTGTCGCCACGAGTTGCCAACCGATGATTCAGAGTATGAGCAGAGGATTCGTGGCGGCGATGCAGCCAATGCAAATACAACTCAATCTGCTGCTGCTGCTGCCAACAATTCCAGTGGTGTTGGTGGGAGTAGGGGGCCAGCTGTTGACATTCAGAGTCAGGCACCACGTACAGTTGAGCGGAGGTTCACCATTTCCTTGCCTCGCCTTTTTAGGGTATTTGGTGGTGCACCCGAGACTAGTAATACTGGGAGTGCAAGTGGAAACAATGATGGGCCTAACTCAGGGAATAGGGGAAGCCATAACTCGGAATCTGAGCCTAGACAAGAAGATCTTGATTGA

Protein Analysis

373

Amino Acids

39.19

Weight (kDa)

4.57

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_19 PF14369 14 - 48 9.5e-15 zinc-ribbon
zf-RING_2 PF13639 221 - 263 4.7e-13 Ring finger domain
zf-C3HC4_2 PF13923 221 - 262 7.4e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 221 - 249 7.2e-08 RING-like zinc finger
zf-C3HC4 PF00097 222 - 262 2e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 667
AccB1I GGYRCC 3 cut(s) 310, 404, 943
AccBSI CCGCTC 3 cut(s) 38, 324, 961
AclWI GGATC 3 cut(s) 461, 474, 557
AcuI CTGAAG 1 cut(s) 229
AcyI GRCGYC 3 cut(s) 198, 311, 348
AfaI GTAC 2 cut(s) 47, 952
AfiI CCNNNNNNNGG 5 cut(s) 257, 337, 994, 1064, 1087
AgsI TTSAA 1 cut(s) 594
AhlI ACTAGT 1 cut(s) 1019
AjnI CCWGG 2 cut(s) 523, 752
AjuI GAANNNNNNNTTGG 4 cut(s) 164, 196, 793, 825
AluBI AGCT 3 cut(s) 550, 691, 923
AluI AGCT 3 cut(s) 550, 691, 923
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 1 cut(s) 1010
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 3 cut(s) 461, 474, 557
Ama87I CYCGRG 3 cut(s) 10, 530, 1013
AoxI GGCC 7 cut(s) 396, 520, 526, 609, 702, 917, 1055
ApaLI GTGCAC 1 cut(s) 1008
ApeKI GCWGC 6 cut(s) 547, 845, 872, 875, 878, 881
AsiSI GCGATCGC 1 cut(s) 67
AspLEI GCGC 2 cut(s) 313, 668
AspS9I GGNCC 6 cut(s) 263, 340, 396, 521, 917, 1055
AsuC2I CCSGG 1 cut(s) 267
AsuHPI GGTGA 1 cut(s) 961
AvaI CYCGRG 3 cut(s) 10, 530, 1013
AvaII GGWCC 2 cut(s) 263, 340
AxyI CCTNAGG 1 cut(s) 392
BaeGI GKGCMC 1 cut(s) 1012
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 3 cut(s) 310, 404, 943
BauI CACGAG 1 cut(s) 791
Bbv12I GWGCWC 1 cut(s) 1012
BbvCI CCTCAGC 1 cut(s) 472
BbvI GCAGC 6 cut(s) 559, 857, 859, 862, 865, 868
BccI CCATC 3 cut(s) 136, 611, 1046
BceAI ACGGC 1 cut(s) 592
BcgI CGANNNNNNTGC 2 cut(s) 812, 846
BciT130I CCWGG 2 cut(s) 525, 754
BcnI CCSGG 1 cut(s) 267
BcoDI GTCTC 1 cut(s) 1010
BcuI ACTAGT 1 cut(s) 1019
BfaI CTAG 2 cut(s) 1020, 1101
BfoI RGCGCY 1 cut(s) 314
BglI GCCNNNNNGGC 1 cut(s) 608
BglII AGATCT 1 cut(s) 1111
BlpI GCTNAGC 1 cut(s) 39
BmcAI AGTACT 1 cut(s) 47
Bme1390I CCNGG 3 cut(s) 267, 525, 754
Bme18I GGWCC 2 cut(s) 263, 340
BmeT110I CYCGRG 3 cut(s) 10, 530, 1013
BmgT120I GGNCC 6 cut(s) 263, 340, 396, 521, 917, 1055
BmiI GGNNCC 6 cut(s) 312, 342, 406, 468, 918, 945
BmrFI CCNGG 3 cut(s) 267, 525, 754
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 3 cut(s) 36, 702, 832
BmuI ACTGGG 1 cut(s) 1038
BpmI CTGGAG 2 cut(s) 205, 356
Bpu10I CCTNAGC 1 cut(s) 472
Bpu1102I GCTNAGC 1 cut(s) 39
BpuMI CCSGG 1 cut(s) 267
BsaAI YACGTR 1 cut(s) 950
BsaHI GRCGYC 3 cut(s) 198, 311, 348
BsaJI CCNNGG 3 cut(s) 9, 523, 752
BsaXI ACNNNNNCTCC 6 cut(s) 272, 302, 444, 474, 1024, 1054
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 337, 994, 1064, 1087
Bse118I RCCGGY 2 cut(s) 257, 586
Bse1I ACTGG 4 cut(s) 58, 617, 894, 1033
Bse21I CCTNAGG 1 cut(s) 392
BseBI CCWGG 2 cut(s) 525, 754
BseDI CCNNGG 3 cut(s) 9, 523, 752
BseGI GGATG 1 cut(s) 540
BseLI CCNNNNNNNGG 5 cut(s) 257, 337, 994, 1064, 1087
BseMII CTCAG 5 cut(s) 53, 486, 674, 1077, 1086
BseNI ACTGG 4 cut(s) 58, 617, 894, 1033
BseSI GKGCMC 1 cut(s) 1012
BseXI GCAGC 6 cut(s) 559, 857, 859, 862, 865, 868
Bsh1285I CGRYCG 1 cut(s) 67
BshFI GGCC 7 cut(s) 398, 522, 528, 611, 704, 919, 1057
BshNI GGYRCC 3 cut(s) 310, 404, 943
BsiEI CGRYCG 1 cut(s) 67
BsiHKAI GWGCWC 1 cut(s) 1012
BsiHKCI CYCGRG 3 cut(s) 10, 530, 1013
BsiSI CCGG 4 cut(s) 24, 258, 266, 587
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 5 cut(s) 257, 337, 994, 1064, 1087
BsmAI GTCTC 1 cut(s) 1010
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 7 cut(s) 398, 522, 528, 611, 704, 919, 1057
BsoBI CYCGRG 3 cut(s) 10, 530, 1013
Bsp1286I GDGCHC 1 cut(s) 1012
Bsp143I GATC 5 cut(s) 64, 100, 466, 562, 1111
Bsp1720I GCTNAGC 1 cut(s) 39
BspANI GGCC 7 cut(s) 398, 522, 528, 611, 704, 919, 1057
BspCNI CTCAG 5 cut(s) 52, 485, 673, 1076, 1087
BspLI GGNNCC 6 cut(s) 312, 342, 406, 468, 918, 945
BspPI GGATC 3 cut(s) 461, 474, 557
BspT107I GGYRCC 3 cut(s) 310, 404, 943
BsrBI CCGCTC 3 cut(s) 38, 324, 961
BsrFI RCCGGY 2 cut(s) 257, 586
BsrI ACTGG 4 cut(s) 58, 617, 894, 1033
BssAI RCCGGY 2 cut(s) 257, 586
BssECI CCNNGG 3 cut(s) 9, 523, 752
BssMI GATC 5 cut(s) 64, 100, 466, 562, 1111
BssNI GRCGYC 3 cut(s) 198, 311, 348
BssSI CACGAG 1 cut(s) 791
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 2 cut(s) 525, 754
Bst4CI ACNGT 2 cut(s) 73, 955
Bst6I CTCTTC 3 cut(s) 132, 248, 302
BstACI GRCGYC 3 cut(s) 198, 311, 348
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBAI YACGTR 1 cut(s) 950
BstC8I GCNNGC 4 cut(s) 552, 581, 722, 921
BstDEI CTNAG 6 cut(s) 39, 392, 472, 660, 1063, 1095
BstENI CCTNNNNNAGG 1 cut(s) 1062
BstF5I GGATG 1 cut(s) 540
BstH2I RGCGCY 1 cut(s) 314
BstHHI GCGC 2 cut(s) 313, 668
BstKTI GATC 5 cut(s) 67, 103, 469, 565, 1114
BstMAI GTCTC 1 cut(s) 1010
BstMBI GATC 5 cut(s) 64, 100, 466, 562, 1111
BstMCI CGRYCG 1 cut(s) 67
BstNI CCWGG 2 cut(s) 525, 754
BstSCI CCNGG 3 cut(s) 265, 523, 752
BstSLI GKGCMC 1 cut(s) 1012
BstV1I GCAGC 6 cut(s) 559, 857, 859, 862, 865, 868
BstX2I RGATCY 2 cut(s) 466, 1111
BstYI RGATCY 2 cut(s) 466, 1111
Bsu36I CCTNAGG 1 cut(s) 392
BsuRI GGCC 7 cut(s) 398, 522, 528, 611, 704, 919, 1057
BtgZI GCGATG 2 cut(s) 13, 855
BtsCI GGATG 1 cut(s) 540
BtsI GCAGTG 2 cut(s) 127, 675
BtsIMutI CAGTG 5 cut(s) 65, 127, 668, 675, 901
Cac8I GCNNGC 4 cut(s) 552, 581, 722, 921
CfoI GCGC 2 cut(s) 313, 668
Cfr10I RCCGGY 2 cut(s) 257, 586
Cfr13I GGNCC 6 cut(s) 263, 340, 396, 521, 917, 1055
CseI GACGC 2 cut(s) 206, 356
Csp6I GTAC 2 cut(s) 46, 951
CspCI CAANNNNNGTGG 2 cut(s) 877, 912
CviAII CATG 1 cut(s) 774
CviQI GTAC 2 cut(s) 46, 951
DdeI CTNAG 6 cut(s) 39, 392, 472, 660, 1063, 1095
DinI GGCGCC 1 cut(s) 312
DpnI GATC 5 cut(s) 66, 102, 468, 564, 1113
DpnII GATC 5 cut(s) 64, 100, 466, 562, 1111
Eam1104I CTCTTC 3 cut(s) 132, 248, 302
EarI CTCTTC 3 cut(s) 132, 248, 302
EciI GGCGGA 2 cut(s) 280, 453
Eco147I AGGCCT 2 cut(s) 528, 611
Eco47I GGWCC 2 cut(s) 263, 340
Eco57I CTGAAG 1 cut(s) 229
Eco81I CCTNAGG 1 cut(s) 392
Eco88I CYCGRG 3 cut(s) 10, 530, 1013
EcoNI CCTNNNNNAGG 1 cut(s) 1062
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 523, 752
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
FaeI CATG 1 cut(s) 777
FaiI YATR 5 cut(s) 730, 768, 775, 819, 1083
FalI AAGNNNNNCTT 3 cut(s) 626, 658, 1098
FaqI GGGAC 1 cut(s) 313
FatI CATG 1 cut(s) 773
FauI CCCGC 3 cut(s) 43, 259, 329
FokI GGATG 1 cut(s) 527
FspBI CTAG 2 cut(s) 1020, 1101
FspI TGCGCA 1 cut(s) 667
GlaI GCGC 2 cut(s) 312, 667
GsuI CTGGAG 2 cut(s) 205, 356
HaeII RGCGCY 1 cut(s) 314
HaeIII GGCC 7 cut(s) 398, 522, 528, 611, 704, 919, 1057
HapII CCGG 4 cut(s) 24, 258, 266, 587
HgaI GACGC 2 cut(s) 206, 356
HhaI GCGC 2 cut(s) 313, 668
Hin1I GRCGYC 3 cut(s) 198, 311, 348
Hin1II CATG 1 cut(s) 777
Hin6I GCGC 2 cut(s) 311, 666
HinP1I GCGC 2 cut(s) 311, 666
HincII GTYRAC 1 cut(s) 928
HindII GTYRAC 1 cut(s) 928
HinfI GANTC 7 cut(s) 647, 653, 680, 809, 829, 937, 1091
HpaII CCGG 4 cut(s) 24, 258, 266, 587
HphI GGTGA 1 cut(s) 961
Hpy166II GTNNAC 3 cut(s) 928, 969, 1010
Hpy188I TCNGA 8 cut(s) 100, 196, 652, 739, 814, 936, 1090, 1096
Hpy188III TCNNGA 3 cut(s) 338, 373, 1115
Hpy8I GTNNAC 3 cut(s) 928, 969, 1010
Hpy99I CGWCG 3 cut(s) 200, 284, 350
HpyAV CCTTC 8 cut(s) 96, 174, 187, 196, 217, 324, 361, 487
HpyCH4III ACNGT 2 cut(s) 73, 955
HpyCH4IV ACGT 1 cut(s) 949
HpyCH4V TGCA 8 cut(s) 120, 385, 720, 766, 845, 854, 1010, 1037
HpyF3I CTNAG 6 cut(s) 39, 392, 472, 660, 1063, 1095
HpySE526I ACGT 1 cut(s) 949
Hsp92I GRCGYC 3 cut(s) 198, 311, 348
Hsp92II CATG 1 cut(s) 777
HspAI GCGC 2 cut(s) 311, 666
KasI GGCGCC 1 cut(s) 310
Kzo9I GATC 5 cut(s) 64, 100, 466, 562, 1111
Lsp1109I GCAGC 6 cut(s) 559, 857, 859, 862, 865, 868
LweI GCATC 3 cut(s) 36, 702, 832
MaeI CTAG 2 cut(s) 1020, 1101
MaeII ACGT 1 cut(s) 949
MalI GATC 5 cut(s) 66, 102, 468, 564, 1113
MbiI CCGCTC 3 cut(s) 38, 324, 961
MboI GATC 5 cut(s) 64, 100, 466, 562, 1111
MboII GAAGA 8 cut(s) 149, 172, 235, 289, 508, 648, 740, 1121
MfeI CAATTG 1 cut(s) 106
MflI RGATCY 2 cut(s) 466, 1111
MhlI GDGCHC 1 cut(s) 1012
MluCI AATT 5 cut(s) 106, 172, 499, 761, 889
Mly113I GGCGCC 1 cut(s) 311
MlyI GAGTC 2 cut(s) 674, 946
MmeI TCCRAC 2 cut(s) 219, 738
MseI TTAA 1 cut(s) 498
MspA1I CMGCKG 3 cut(s) 225, 435, 923
MspI CCGG 4 cut(s) 24, 258, 266, 587
MspR9I CCNGG 3 cut(s) 267, 525, 754
MunI CAATTG 1 cut(s) 106
MvaI CCWGG 2 cut(s) 525, 754
NarI GGCGCC 1 cut(s) 311
NciI CCSGG 1 cut(s) 267
NdeII GATC 5 cut(s) 64, 100, 466, 562, 1111
NlaIII CATG 1 cut(s) 777
NlaIV GGNNCC 6 cut(s) 312, 342, 406, 468, 918, 945
NmeAIII GCCGAG 1 cut(s) 580
NsbI TGCGCA 1 cut(s) 667
PaeR7I CTCGAG 1 cut(s) 530
PceI AGGCCT 2 cut(s) 528, 611
PfeI GAWTC 5 cut(s) 647, 653, 809, 829, 1091
PfoI TCCNGGA 1 cut(s) 265
Ple19I CGATCG 1 cut(s) 67
PleI GAGTC 2 cut(s) 674, 945
PluTI GGCGCC 1 cut(s) 314
PpsI GAGTC 2 cut(s) 674, 945
Ppu21I YACGTR 1 cut(s) 950
PpuMI RGGWCCY 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 2 cut(s) 523, 752
PspGI CCWGG 2 cut(s) 523, 752
PspN4I GGNNCC 6 cut(s) 312, 342, 406, 468, 918, 945
PspPI GGNCC 6 cut(s) 263, 340, 396, 521, 917, 1055
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 1 cut(s) 530
PsuI RGATCY 2 cut(s) 466, 1111
PvuI CGATCG 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 923
RgaI GCGATCGC 1 cut(s) 67
RsaI GTAC 2 cut(s) 47, 952
RsaNI GTAC 2 cut(s) 46, 951
SaqAI TTAA 1 cut(s) 498
Sau3AI GATC 5 cut(s) 64, 100, 466, 562, 1111
Sau96I GGNCC 6 cut(s) 263, 340, 396, 521, 917, 1055
ScaI AGTACT 1 cut(s) 47
SchI GAGTC 2 cut(s) 674, 946
ScrFI CCNGG 3 cut(s) 267, 525, 754
SduI GDGCHC 1 cut(s) 1012
SetI ASST 7 cut(s) 98, 152, 552, 693, 925, 952, 968
SfaAI GCGATCGC 1 cut(s) 67
SfaNI GCATC 3 cut(s) 36, 702, 832
SfoI GGCGCC 1 cut(s) 312
Sfr274I CTCGAG 1 cut(s) 530
SgfI GCGATCGC 1 cut(s) 67
SgrAI CRCCGGYG 1 cut(s) 257
SinI GGWCC 2 cut(s) 263, 340
SlaI CTCGAG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SpeI ACTAGT 1 cut(s) 1019
Sse9I AATT 5 cut(s) 106, 172, 499, 761, 889
SseBI AGGCCT 2 cut(s) 528, 611
SspDI GGCGCC 1 cut(s) 310
SspMI CTAG 2 cut(s) 1020, 1101
StuI AGGCCT 2 cut(s) 528, 611
StyD4I CCNGG 3 cut(s) 265, 523, 752
TaaI ACNGT 2 cut(s) 73, 955
TaiI ACGT 1 cut(s) 952
TaqI TCGA 4 cut(s) 135, 354, 531, 606
TasI AATT 5 cut(s) 106, 172, 499, 761, 889
TatI WGTACW 1 cut(s) 45
TauI GCSGC 6 cut(s) 18, 129, 228, 257, 438, 840
TfiI GAWTC 5 cut(s) 647, 653, 809, 829, 1091
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TscAI CASTG 5 cut(s) 65, 127, 668, 675, 901
TseI GCWGC 6 cut(s) 547, 845, 872, 875, 878, 881
TspGWI ACGGA 1 cut(s) 470
TspRI CASTG 5 cut(s) 65, 127, 668, 675, 901
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 2 cut(s) 263, 340
XagI CCTNNNNNAGG 1 cut(s) 1062
XhoI CTCGAG 1 cut(s) 530
XspI CTAG 2 cut(s) 1020, 1101
ZrmI AGTACT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.