MD15G1036500.v1.1

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013409
Physical Location & Seq
Forward (+)
2548128 .. 2549263
1136 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1036500.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCGAGACCGCAACAGCGATATCGAGGCGTCCGACAAAGGCATTGGGGCTCTTGGGTCTCCGAGATCCGCCACCCTTTATTGAAGACTAGAATTTGGCTGGGAACATTCGAAACGGCGGAAGACGCGGCGAGAGCTTACGACGAAGCGGCGAGGCTCATGTGCGGGCCGAGGGCAAGAACAAACTTCCCTTATAACCCAAATGCGTCTCAGTCCTCGGCCTCCAAACTTCTCTCGGCAACTTTGACAGCAAAACTGCACAGATGTTACATGGCTTCACTCCAAATGACCAAAACTTCACTACAAGAGCCAAAAAAGCCGAAAACTTTAGTGTCATCGGGCGCGAAAATGCCAGAGATGATCAGACCGGAAATTCAGCAAGGAGCAGAGGAAAATTGGATTGTGAGGGAAGTTAAAGTAGAGAGCAGCAGCCAGCAGTTCAAGCCACTTGAAGATGATCACATTGAGCAGATGATTGAGGAGTTACTGCATTACGGGTCCATTGAGTTATGCTCTGTTGTGCCACCACAACCTACCTAA

Protein Analysis

180

Amino Acids

20.45

Weight (kDa)

9.03

Isoelectric Point (pI)

63.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 7 - 56 1.6e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25190
fragaria_vesca FvH4_2g40810
malus_domestica MD08G1060000.v1.1 MD15G1036500.v1.1
prunus_persica Prupe.1G390800_v2.0.a1
pyrus_communis pycom08g05000 pycom15g03390
rosa_chinensis RchiOBHm_Chr6g0299771
rosa_laevigata RLG00000011390
rosa_multiflora Rmu_co8448481.1_g000001 Rmu_sc0002895.1_g000027
rosa_roxburghii Rroxscaffold_7G00168390
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG403100 Rh6BG411600 Rh6CG417700 Rh6DG404500
rosa_wichuraiana Rw6G035280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AccII CGCG 2 cut(s) 128, 344
AciI CCGC 6 cut(s) 11, 70, 119, 128, 149, 165
AclWI GGATC 1 cut(s) 61
AcsI RAATTY 2 cut(s) 93, 372
AcyI GRCGYC 1 cut(s) 30
AgsI TTSAA 3 cut(s) 85, 442, 452
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw26I GTCTC 2 cut(s) 64, 213
AlwI GGATC 1 cut(s) 61
AoxI GGCC 2 cut(s) 167, 219
ApeKI GCWGC 2 cut(s) 426, 429
ApoI RAATTY 2 cut(s) 93, 372
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 167, 498
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 498
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 2 cut(s) 92, 129
BbvI GCAGC 2 cut(s) 438, 441
BceAI ACGGC 1 cut(s) 132
BclI TGATCA 2 cut(s) 360, 457
BcoDI GTCTC 2 cut(s) 64, 213
BfaI CTAG 1 cut(s) 90
BisI GCNGC 4 cut(s) 129, 150, 427, 430
BlsI GCNGC 4 cut(s) 130, 151, 428, 431
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 167, 498
BmiI GGNNCC 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 92, 129
BplI GAGNNNNNCTC 2 cut(s) 497, 529
Bpu14I TTCGAA 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 2 cut(s) 170, 216
BsaWI WCCGGW 1 cut(s) 367
BseDI CCNNGG 2 cut(s) 170, 216
BseMII CTCAG 1 cut(s) 224
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 2 cut(s) 438, 441
BseYI CCCAGC 1 cut(s) 100
BsgI GTGCAG 1 cut(s) 242
Bsh1236I CGCG 2 cut(s) 128, 344
BshFI GGCC 2 cut(s) 169, 221
BsiSI CCGG 1 cut(s) 368
BsmAI GTCTC 2 cut(s) 64, 213
BsmBI CGTCTC 1 cut(s) 213
BsnI GGCC 2 cut(s) 169, 221
Bso31I GGTCTC 1 cut(s) 64
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 53
Bsp143I GATC 3 cut(s) 66, 360, 457
BspACI CCGC 6 cut(s) 11, 70, 119, 128, 149, 165
BspANI GGCC 2 cut(s) 169, 221
BspCNI CTCAG 1 cut(s) 223
BspFNI CGCG 2 cut(s) 128, 344
BspLI GGNNCC 1 cut(s) 499
BspPI GGATC 1 cut(s) 61
BspT104I TTCGAA 1 cut(s) 111
BspTNI GGTCTC 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 170, 216
BssMI GATC 3 cut(s) 66, 360, 457
BssNI GRCGYC 1 cut(s) 30
BstACI GRCGYC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 167, 434
BstDEI CTNAG 1 cut(s) 210
BstFNI CGCG 2 cut(s) 128, 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 3 cut(s) 69, 363, 460
BstMAI GTCTC 2 cut(s) 64, 213
BstMBI GATC 3 cut(s) 66, 360, 457
BstMWI GCNNNNNNNGC 4 cut(s) 125, 134, 316, 442
BstUI CGCG 2 cut(s) 128, 344
BstV1I GCAGC 2 cut(s) 438, 441
BstV2I GAAGAC 2 cut(s) 92, 129
BstX2I RGATCY 1 cut(s) 66
BstYI RGATCY 1 cut(s) 66
BsuRI GGCC 2 cut(s) 169, 221
Cac8I GCNNGC 2 cut(s) 167, 434
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 167, 498
CseI GACGC 3 cut(s) 19, 134, 195
CviAII CATG 2 cut(s) 160, 271
DdeI CTNAG 1 cut(s) 210
DpnI GATC 3 cut(s) 68, 362, 459
DpnII GATC 3 cut(s) 66, 360, 457
EciI GGCGGA 2 cut(s) 59, 134
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 64
Eco32I GATATC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 498
EcoRV GATATC 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 53
Esp3I CGTCTC 1 cut(s) 213
FaeI CATG 2 cut(s) 163, 274
FaiI YATR 4 cut(s) 161, 195, 272, 511
FatI CATG 2 cut(s) 159, 270
FauI CCCGC 1 cut(s) 158
FbaI TGATCA 2 cut(s) 360, 457
Fnu4HI GCNGC 4 cut(s) 129, 150, 427, 430
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 4 cut(s) 129, 150, 427, 430
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 343
GluI GCNGC 4 cut(s) 129, 150, 427, 430
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 169, 221
HapII CCGG 1 cut(s) 368
HgaI GACGC 3 cut(s) 19, 134, 195
HhaI GCGC 1 cut(s) 344
Hin1I GRCGYC 1 cut(s) 30
Hin1II CATG 2 cut(s) 163, 274
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HpaII CCGG 1 cut(s) 368
Hpy188I TCNGA 3 cut(s) 35, 64, 365
Hpy99I CGWCG 1 cut(s) 146
HpyCH4V TGCA 2 cut(s) 259, 490
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 134, 316, 442
HpyF3I CTNAG 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 30
Hsp92II CATG 2 cut(s) 163, 274
HspAI GCGC 1 cut(s) 342
Ksp22I TGATCA 2 cut(s) 360, 457
Kzo9I GATC 3 cut(s) 66, 360, 457
LmnI GCTCC 1 cut(s) 383
LpnPI CCDG 4 cut(s) 86, 366, 381, 446
Lsp1109I GCAGC 2 cut(s) 438, 441
MaeI CTAG 1 cut(s) 90
MaeIII GTNAC 2 cut(s) 266, 483
MalI GATC 3 cut(s) 68, 362, 459
MboI GATC 3 cut(s) 66, 360, 457
MboII GAAGA 3 cut(s) 97, 134, 464
MflI RGATCY 1 cut(s) 66
MhlI GDGCHC 1 cut(s) 53
MluCI AATT 3 cut(s) 93, 372, 394
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 8 cut(s) 20, 147, 165, 226, 232, 382, 399, 472
MseI TTAA 1 cut(s) 414
MspI CCGG 1 cut(s) 368
MvnI CGCG 2 cut(s) 128, 344
MwoI GCNNNNNNNGC 4 cut(s) 125, 134, 316, 442
NdeII GATC 3 cut(s) 66, 360, 457
NlaIII CATG 2 cut(s) 163, 274
NlaIV GGNNCC 1 cut(s) 499
NmeAIII GCCGAG 3 cut(s) 195, 197, 215
NspV TTCGAA 1 cut(s) 111
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PkrI GCNGC 4 cut(s) 130, 151, 428, 431
PsiI TTATAA 1 cut(s) 195
PspFI CCCAGC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 499
PspPI GGNCC 2 cut(s) 167, 498
PsuI RGATCY 1 cut(s) 66
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 4 cut(s) 129, 150, 427, 430
Sau3AI GATC 3 cut(s) 66, 360, 457
Sau96I GGNCC 2 cut(s) 167, 498
SduI GDGCHC 1 cut(s) 53
SetI ASST 3 cut(s) 139, 535, 539
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 1 cut(s) 498
Sse9I AATT 3 cut(s) 93, 372, 394
SsiI CCGC 6 cut(s) 11, 70, 119, 128, 149, 165
SspMI CTAG 1 cut(s) 90
TaqI TCGA 2 cut(s) 25, 111
TasI AATT 3 cut(s) 93, 372, 394
TauI GCSGC 2 cut(s) 131, 152
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TseI GCWGC 2 cut(s) 426, 429
VpaK11BI GGWCC 1 cut(s) 498
XapI RAATTY 2 cut(s) 93, 372
XspI CTAG 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.