Rh6DG404500

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0013409
Physical Location & Seq
Reverse (-)
59231764 .. 59233645
1882 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG404500.1

Sequence Viewer

Length: 831 bp
ATGCAGGGTTTACACACGAAAACTCAACTGATGGGACCCATTCTCTTCAATTTATTTGTCTTTTGGGCAATCCCCAACTACAATTTAAGGGCCAGAGAGGAGACTAGTCTCTTCCCTTCCTCAAAGACACCAGCTTCACTCTCACAAAGAAACACACAGACATTTCCCATTTTCCACCATACACTTTCAGAAAGAAACGTTCTTTTGCTTTGCTGCACAGCAATGGCAAGACCCCAGCAACGATACCGAGGCGTACGACAAAGGCATTGGGGCTCTTGGGTCTCTGAGATTCGCCACCCTTTACTAAAGACTAGAATTTGGTTGGGAACATTTGAGACGGCTGAAGACGCAGCCAGGGCTTATGATGAAGCTGCCAGGCTTATGTGCGGACCTAGAGCCAGGACCAACTTCCCTTACAACCCAAATGCGTCTCAGTCCTCTGCCTCAAAGCTTCTCTCCCAAACTTTGACGGCTAAACTCCACAGGTGCTACATGGCTTCACTCCAAATGACCAAATCAACCCCACTCAAGCCCCAGCTGTCCTATGTTGACACAAAAAATGTTGGCATTGCTGAGAATGAGAAACCCAGCATTGAAGTGCCAGAGAAGAAACCAGCACTAACAGCTGAGCCTAAACAAGAACAAGAGCAAGAGCCAAATTGGGGATTTGTGAATAAAGTCAAAGTGGAGAGCACCCAAGATGAAGAATCCATAGAGCCTCTTGAAGATGACCACATTGAGCAGATGATTGAGGAGTTGCTCCATTATGGGTCCATTGAGCTATGCTCTGTTGTCACACCACCACAACCACCTCAGGCCTCTCTAATTTAA

Protein Analysis

276

Amino Acids

31.43

Weight (kDa)

7.09

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 81 - 130 3.5e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25190
fragaria_vesca FvH4_2g40810
malus_domestica MD08G1060000.v1.1 MD15G1036500.v1.1
prunus_persica Prupe.1G390800_v2.0.a1
pyrus_communis pycom08g05000 pycom15g03390
rosa_chinensis RchiOBHm_Chr6g0299771
rosa_laevigata RLG00000011390
rosa_multiflora Rmu_co8448481.1_g000001 Rmu_sc0002895.1_g000027
rosa_roxburghii Rroxscaffold_7G00168390
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG403100 Rh6BG411600 Rh6CG417700 Rh6DG404500
rosa_wichuraiana Rw6G035280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 387
AclI AACGTT 1 cut(s) 198
AcsI RAATTY 1 cut(s) 315
AcuI CTGAAG 1 cut(s) 363
AfaI GTAC 1 cut(s) 255
AfiI CCNNNNNNNGG 1 cut(s) 662
AgsI TTSAA 3 cut(s) 49, 596, 725
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 3 cut(s) 353, 374, 398
AluBI AGCT 6 cut(s) 134, 371, 451, 538, 626, 781
AluI AGCT 6 cut(s) 134, 371, 451, 538, 626, 781
Alw21I GWGCWC 1 cut(s) 695
Alw26I GTCTC 5 cut(s) 95, 113, 286, 329, 435
AoxI GGCC 2 cut(s) 90, 816
ApeKI GCWGC 3 cut(s) 213, 350, 371
ApoI RAATTY 1 cut(s) 315
AspS9I GGNCC 5 cut(s) 35, 90, 389, 402, 771
AvaII GGWCC 4 cut(s) 35, 389, 402, 771
AxyI CCTNAGG 1 cut(s) 813
BanII GRGCYC 1 cut(s) 275
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 1 cut(s) 695
BbvI GCAGC 3 cut(s) 200, 358, 362
BccI CCATC 1 cut(s) 25
BceAI ACGGC 2 cut(s) 354, 486
BciT130I CCWGG 3 cut(s) 355, 376, 400
BcoDI GTCTC 5 cut(s) 95, 113, 286, 329, 435
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 3 cut(s) 105, 312, 393
BisI GCNGC 3 cut(s) 214, 351, 372
BlpI GCTNAGC 1 cut(s) 627
BlsI GCNGC 3 cut(s) 215, 352, 373
Bme1390I CCNGG 3 cut(s) 355, 376, 400
Bme18I GGWCC 4 cut(s) 35, 389, 402, 771
BmgT120I GGNCC 5 cut(s) 35, 90, 389, 402, 771
BmiI GGNNCC 3 cut(s) 36, 37, 772
BmrFI CCNGG 3 cut(s) 355, 376, 400
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 770, 802
Bpu1102I GCTNAGC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 512
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 247, 354
Bsc4I CCNNNNNNNGG 1 cut(s) 662
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 2 cut(s) 228, 567
BseBI CCWGG 3 cut(s) 355, 376, 400
BseDI CCNNGG 2 cut(s) 247, 354
BseLI CCNNNNNNNGG 1 cut(s) 662
BseMI GCAATG 2 cut(s) 228, 567
BseMII CTCAG 5 cut(s) 276, 446, 564, 618, 827
BseRI GAGGAG 2 cut(s) 113, 767
BseXI GCAGC 3 cut(s) 200, 358, 362
BseYI CCCAGC 3 cut(s) 234, 534, 587
BsgI GTGCAG 1 cut(s) 199
BshFI GGCC 2 cut(s) 92, 818
BsiHKAI GWGCWC 1 cut(s) 695
BsiWI CGTACG 1 cut(s) 253
BslFI GGGAC 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 662
BsmAI GTCTC 5 cut(s) 95, 113, 286, 329, 435
BsmBI CGTCTC 2 cut(s) 329, 435
BsmFI GGGAC 1 cut(s) 48
BsnI GGCC 2 cut(s) 92, 818
Bso31I GGTCTC 1 cut(s) 286
Bsp1286I GDGCHC 2 cut(s) 275, 695
Bsp1720I GCTNAGC 1 cut(s) 627
BspACI CCGC 1 cut(s) 387
BspANI GGCC 2 cut(s) 92, 818
BspCNI CTCAG 5 cut(s) 277, 445, 565, 619, 826
BspLI GGNNCC 3 cut(s) 36, 37, 772
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 2 cut(s) 228, 567
BssECI CCNNGG 2 cut(s) 247, 354
Bst2UI CCWGG 3 cut(s) 355, 376, 400
Bst6I CTCTTC 2 cut(s) 50, 116
BstDEI CTNAG 5 cut(s) 285, 432, 573, 627, 813
BstMAI GTCTC 5 cut(s) 95, 113, 286, 329, 435
BstMWI GCNNNNNNNGC 3 cut(s) 347, 356, 623
BstNI CCWGG 3 cut(s) 355, 376, 400
BstSCI CCNGG 3 cut(s) 353, 374, 398
BstV1I GCAGC 3 cut(s) 200, 358, 362
BstV2I GAAGAC 1 cut(s) 351
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 2 cut(s) 92, 818
Cfr13I GGNCC 5 cut(s) 35, 90, 389, 402, 771
CseI GACGC 2 cut(s) 356, 417
Csp6I GTAC 1 cut(s) 254
CviAII CATG 1 cut(s) 493
CviQI GTAC 1 cut(s) 254
DdeI CTNAG 5 cut(s) 285, 432, 573, 627, 813
Eam1104I CTCTTC 2 cut(s) 50, 116
EarI CTCTTC 2 cut(s) 50, 116
Eco147I AGGCCT 1 cut(s) 818
Eco24I GRGCYC 1 cut(s) 275
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 4 cut(s) 35, 389, 402, 771
Eco57I CTGAAG 1 cut(s) 363
Eco81I CCTNAGG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 35
EcoRII CCWGG 3 cut(s) 353, 374, 398
EcoT38I GRGCYC 1 cut(s) 275
Esp3I CGTCTC 2 cut(s) 329, 435
FaeI CATG 1 cut(s) 496
FaiI YATR 8 cut(s) 180, 363, 383, 494, 546, 713, 768, 784
FaqI GGGAC 1 cut(s) 48
FatI CATG 1 cut(s) 492
Fnu4HI GCNGC 3 cut(s) 214, 351, 372
FriOI GRGCYC 1 cut(s) 275
Fsp4HI GCNGC 3 cut(s) 214, 351, 372
FspBI CTAG 3 cut(s) 105, 312, 393
GluI GCNGC 3 cut(s) 214, 351, 372
GsaI CCCAGC 3 cut(s) 238, 538, 591
HaeIII GGCC 2 cut(s) 92, 818
HgaI GACGC 2 cut(s) 356, 417
Hin1II CATG 1 cut(s) 496
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HindIII AAGCTT 1 cut(s) 449
HinfI GANTC 2 cut(s) 289, 707
Hpy166II GTNNAC 2 cut(s) 11, 550
Hpy188I TCNGA 2 cut(s) 190, 286
Hpy188III TCNNGA 1 cut(s) 722
Hpy8I GTNNAC 2 cut(s) 11, 550
HpyAV CCTTC 1 cut(s) 126
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 2 cut(s) 4, 216
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 356, 623
HpyF3I CTNAG 5 cut(s) 285, 432, 573, 627, 813
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 1 cut(s) 496
KflI GGGWCCC 1 cut(s) 35
LmnI GCTCC 1 cut(s) 765
Lsp1109I GCAGC 3 cut(s) 200, 358, 362
MaeI CTAG 3 cut(s) 105, 312, 393
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 1 cut(s) 793
MboII GAAGA 6 cut(s) 37, 103, 356, 619, 716, 737
MhlI GDGCHC 2 cut(s) 275, 695
MluCI AATT 5 cut(s) 49, 82, 315, 658, 825
MnlI CCTC 9 cut(s) 91, 130, 242, 448, 454, 729, 745, 822, 829
MseI TTAA 2 cut(s) 86, 829
MslI CAYNNNNRTG 2 cut(s) 221, 596
MspA1I CMGCKG 2 cut(s) 538, 626
MspR9I CCNGG 3 cut(s) 355, 376, 400
MvaI CCWGG 3 cut(s) 355, 376, 400
MwoI GCNNNNNNNGC 3 cut(s) 347, 356, 623
NlaIII CATG 1 cut(s) 496
NlaIV GGNNCC 3 cut(s) 36, 37, 772
NmuCI GTSAC 1 cut(s) 793
PceI AGGCCT 1 cut(s) 818
PfeI GAWTC 2 cut(s) 289, 707
Pfl23II CGTACG 1 cut(s) 253
PkrI GCNGC 3 cut(s) 215, 352, 373
PpuMI RGGWCCY 1 cut(s) 35
Psp1406I AACGTT 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 3 cut(s) 353, 374, 398
PspFI CCCAGC 3 cut(s) 234, 534, 587
PspGI CCWGG 3 cut(s) 353, 374, 398
PspLI CGTACG 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 36, 37, 772
PspPI GGNCC 5 cut(s) 35, 90, 389, 402, 771
PspPPI RGGWCCY 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 538, 626
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
RseI CAYNNNNRTG 2 cut(s) 221, 596
SaqAI TTAA 2 cut(s) 86, 829
SatI GCNGC 3 cut(s) 214, 351, 372
Sau96I GGNCC 5 cut(s) 35, 90, 389, 402, 771
ScrFI CCNGG 3 cut(s) 355, 376, 400
SduI GDGCHC 2 cut(s) 275, 695
SinI GGWCC 4 cut(s) 35, 389, 402, 771
SmiMI CAYNNNNRTG 2 cut(s) 221, 596
SmlI CTYRAG 1 cut(s) 527
SmoI CTYRAG 1 cut(s) 527
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 5 cut(s) 49, 82, 315, 658, 825
SseBI AGGCCT 1 cut(s) 818
SsiI CCGC 1 cut(s) 387
SspMI CTAG 3 cut(s) 105, 312, 393
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 3 cut(s) 353, 374, 398
TaiI ACGT 1 cut(s) 201
TasI AATT 5 cut(s) 49, 82, 315, 658, 825
TfiI GAWTC 2 cut(s) 289, 707
Tru1I TTAA 2 cut(s) 86, 829
Tru9I TTAA 2 cut(s) 86, 829
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 3 cut(s) 213, 350, 371
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 2 cut(s) 381, 717
VpaK11BI GGWCC 4 cut(s) 35, 389, 402, 771
XapI RAATTY 1 cut(s) 315
XspI CTAG 3 cut(s) 105, 312, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.