Rroxscaffold_7G00168390

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013409
Physical Location & Seq
Forward (+)
9267068 .. 9268132
1065 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_7G00168390.1

Sequence Viewer

Length: 621 bp
ATGGCAAGACCCCAGCAACGATACCGAGGCGTACGACAAAGGCATTGGGGCTCTTGGGTCTCTGAGATTCGCCACCCTTTACTAAAGACTAGAATTTGGTTGGGGACATTTGAGACGGCTGAAGACGCAGCCAGGGCCTATGATGAAGCTGCCAGGCTTATGTGCGGACCTAGAGCCAGGACCAACTTCCCTTACAACCCAAATGCGTCTCAGTCCTCTGCCTCAAAGCTTCTCTCCCAAACTTTGACGGCTAAACTCCACAGGTGCTACATGGCTTCACTCCAAATGACCAAATCAACCCCACTCAAGCAGCCCAGCAAGCCCCAGCTGTCCTATGTTGACACAAAAAATGTTGGCATTGCTGAGAATGAGAAACCCAGCATTGAAGTGCCAGAGAAGAAACCAGCACTAACAGCTGAGCCTAAGCAAGAACAAGAGCAAGAACCAAATTGGGGATTTGTGAATAAAGTCAAAGTGGAGAGCACCCAAGATGAAGAATCCATAGAGCCTCTTGAAGATGACCACATTGAGCAGATGATTGAGGAGTTGCTCCATTATGGGTCCATTGAGCTATGCTCTGTTGTCACACCACCACAACCACCTCAGGCCTCTCTAATTTAA

Protein Analysis

206

Amino Acids

23.41

Weight (kDa)

6.24

Isoelectric Point (pI)

62.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 7 - 56 2.1e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25190
fragaria_vesca FvH4_2g40810
malus_domestica MD08G1060000.v1.1 MD15G1036500.v1.1
prunus_persica Prupe.1G390800_v2.0.a1
pyrus_communis pycom08g05000 pycom15g03390
rosa_chinensis RchiOBHm_Chr6g0299771
rosa_laevigata RLG00000011390
rosa_multiflora Rmu_co8448481.1_g000001 Rmu_sc0002895.1_g000027
rosa_roxburghii Rroxscaffold_7G00168390
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG403100 Rh6BG411600 Rh6CG417700 Rh6DG404500
rosa_wichuraiana Rw6G035280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 452
AgsI TTSAA 2 cut(s) 386, 515
AjnI CCWGG 3 cut(s) 131, 152, 176
AluBI AGCT 5 cut(s) 149, 229, 328, 416, 571
AluI AGCT 5 cut(s) 149, 229, 328, 416, 571
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 3 cut(s) 64, 107, 213
AoxI GGCC 2 cut(s) 135, 606
ApeKI GCWGC 3 cut(s) 128, 149, 310
ApoI RAATTY 1 cut(s) 93
AspS9I GGNCC 4 cut(s) 135, 167, 180, 561
AvaII GGWCC 3 cut(s) 167, 180, 561
AxyI CCTNAGG 1 cut(s) 603
BanII GRGCYC 1 cut(s) 53
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 485
BbvI GCAGC 3 cut(s) 136, 140, 322
BceAI ACGGC 2 cut(s) 132, 264
BciT130I CCWGG 3 cut(s) 133, 154, 178
BcoDI GTCTC 3 cut(s) 64, 107, 213
BfaI CTAG 2 cut(s) 90, 171
BisI GCNGC 3 cut(s) 129, 150, 311
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 130, 151, 312
Bme1390I CCNGG 3 cut(s) 133, 154, 178
Bme18I GGWCC 3 cut(s) 167, 180, 561
BmgT120I GGNCC 4 cut(s) 135, 167, 180, 561
BmiI GGNNCC 1 cut(s) 562
BmrFI CCNGG 3 cut(s) 133, 154, 178
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 560, 592
Bpu10I CCTNAGC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 290
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 2 cut(s) 25, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 3 cut(s) 133, 154, 178
BseDI CCNNGG 2 cut(s) 25, 132
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 5 cut(s) 54, 224, 354, 408, 617
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 3 cut(s) 136, 140, 322
BseYI CCCAGC 4 cut(s) 12, 314, 324, 377
BshFI GGCC 2 cut(s) 137, 608
BsiHKAI GWGCWC 1 cut(s) 485
BsiWI CGTACG 1 cut(s) 31
BslFI GGGAC 1 cut(s) 118
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 3 cut(s) 64, 107, 213
BsmBI CGTCTC 2 cut(s) 107, 213
BsmFI GGGAC 1 cut(s) 118
BsnI GGCC 2 cut(s) 137, 608
Bso31I GGTCTC 1 cut(s) 64
Bsp1286I GDGCHC 2 cut(s) 53, 485
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 1 cut(s) 165
BspANI GGCC 2 cut(s) 137, 608
BspCNI CTCAG 5 cut(s) 55, 223, 355, 409, 616
BspLI GGNNCC 1 cut(s) 562
BspTNI GGTCTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 25, 132
Bst2UI CCWGG 3 cut(s) 133, 154, 178
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 6 cut(s) 63, 210, 363, 417, 423, 603
BstMAI GTCTC 3 cut(s) 64, 107, 213
BstMWI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
BstNI CCWGG 3 cut(s) 133, 154, 178
BstSCI CCNGG 3 cut(s) 131, 152, 176
BstV1I GCAGC 3 cut(s) 136, 140, 322
BstV2I GAAGAC 1 cut(s) 129
Bsu36I CCTNAGG 1 cut(s) 603
BsuRI GGCC 2 cut(s) 137, 608
Cac8I GCNNGC 1 cut(s) 320
Cfr13I GGNCC 4 cut(s) 135, 167, 180, 561
CseI GACGC 2 cut(s) 134, 195
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 271
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 6 cut(s) 63, 210, 363, 417, 423, 603
Eco147I AGGCCT 1 cut(s) 608
Eco24I GRGCYC 1 cut(s) 53
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 3 cut(s) 167, 180, 561
Eco57I CTGAAG 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 603
EcoO109I RGGNCCY 1 cut(s) 135
EcoRII CCWGG 3 cut(s) 131, 152, 176
EcoT38I GRGCYC 1 cut(s) 53
Esp3I CGTCTC 2 cut(s) 107, 213
FaeI CATG 1 cut(s) 274
FaiI YATR 7 cut(s) 141, 161, 272, 336, 503, 558, 574
FaqI GGGAC 1 cut(s) 118
FatI CATG 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 129, 150, 311
FriOI GRGCYC 1 cut(s) 53
Fsp4HI GCNGC 3 cut(s) 129, 150, 311
FspBI CTAG 2 cut(s) 90, 171
GluI GCNGC 3 cut(s) 129, 150, 311
GsaI CCCAGC 4 cut(s) 16, 318, 328, 381
HaeIII GGCC 2 cut(s) 137, 608
HgaI GACGC 2 cut(s) 134, 195
Hin1II CATG 1 cut(s) 274
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 67, 497
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
HpyF3I CTNAG 6 cut(s) 63, 210, 363, 417, 423, 603
Hsp92II CATG 1 cut(s) 274
LmnI GCTCC 1 cut(s) 555
Lsp1109I GCAGC 3 cut(s) 136, 140, 322
MaeI CTAG 2 cut(s) 90, 171
MaeIII GTNAC 1 cut(s) 583
MboII GAAGA 4 cut(s) 134, 409, 506, 527
MhlI GDGCHC 2 cut(s) 53, 485
MluCI AATT 3 cut(s) 93, 448, 615
MnlI CCTC 7 cut(s) 20, 226, 232, 519, 535, 612, 619
MseI TTAA 1 cut(s) 619
MslI CAYNNNNRTG 1 cut(s) 386
MspA1I CMGCKG 2 cut(s) 328, 416
MspR9I CCNGG 3 cut(s) 133, 154, 178
MvaI CCWGG 3 cut(s) 133, 154, 178
MwoI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 1 cut(s) 562
NmuCI GTSAC 1 cut(s) 583
PceI AGGCCT 1 cut(s) 608
PfeI GAWTC 2 cut(s) 67, 497
Pfl23II CGTACG 1 cut(s) 31
PkrI GCNGC 3 cut(s) 130, 151, 312
Psp6I CCWGG 3 cut(s) 131, 152, 176
PspFI CCCAGC 4 cut(s) 12, 314, 324, 377
PspGI CCWGG 3 cut(s) 131, 152, 176
PspLI CGTACG 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 562
PspPI GGNCC 4 cut(s) 135, 167, 180, 561
PvuII CAGCTG 2 cut(s) 328, 416
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 1 cut(s) 619
SatI GCNGC 3 cut(s) 129, 150, 311
Sau96I GGNCC 4 cut(s) 135, 167, 180, 561
ScrFI CCNGG 3 cut(s) 133, 154, 178
SduI GDGCHC 2 cut(s) 53, 485
SetI ASST 8 cut(s) 151, 172, 231, 266, 330, 418, 573, 604
SinI GGWCC 3 cut(s) 167, 180, 561
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 3 cut(s) 93, 448, 615
SseBI AGGCCT 1 cut(s) 608
SsiI CCGC 1 cut(s) 165
SspMI CTAG 2 cut(s) 90, 171
StuI AGGCCT 1 cut(s) 608
StyD4I CCNGG 3 cut(s) 131, 152, 176
TasI AATT 3 cut(s) 93, 448, 615
TfiI GAWTC 2 cut(s) 67, 497
Tru1I TTAA 1 cut(s) 619
Tru9I TTAA 1 cut(s) 619
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 3 cut(s) 128, 149, 310
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 2 cut(s) 159, 507
VpaK11BI GGWCC 3 cut(s) 167, 180, 561
XapI RAATTY 1 cut(s) 93
XspI CTAG 2 cut(s) 90, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.