RLG00000011390

Ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013409
Physical Location & Seq
Forward (+)
9074738 .. 9076039
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011390

Sequence Viewer

Length: 624 bp
ATGGCAAGACCCCAGCAACGATACCGAGGCGTACGACAAAGGCATTGGGGTTCTTGGGTCTCTGAGATTCGCCACCCTTTACTAAAGACTAGAATTTGGTTGGGAACATTTGAGACGGCTGAAGACGCAGCCCGGGCTTATGATGAAGCTGCCAGGCTTATGTGCGGACCTAGAGCCAGGACCAACTTCCCTTACAACCCAAATGCGTCTCAGTCCTCTGCCTCAAAGCTTCTCTCCCAAACTTTGACGGCTAAACTCCACAGGTGCTACATGGCTTCACTCCAAATGACCAAATCAACCCCACTCAAGCAGCCCAGCAAGCCCCAGCTGTCCTATGTTGACACAAAAAATGTTGGCATTGCTGAGAATGAGAAACCCAGCATTGAAGTGCCAGAGAAGAAACCAGCAATAACAGCTGAGCCTAAACAAGAAGAACAAGAGCAAGAGCCAAATTGGGGATTTGTGAATAAAGTCAAAGTGGAGAGCACCCAAGATGAAGAATCCATAGAGCCTCTTGAAGATGACCACATTGAGCAGATGATTGAGGAGTTGCTCCATTATGGGTCCATTGAGCTATGCTCTGTTGTCACACCACCACAACCACCTCAGGCCTCTCTAATTTAA

Protein Analysis

208

Amino Acids

23.54

Weight (kDa)

6.0

Isoelectric Point (pI)

64.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 7 - 56 2.1e-12 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013409)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25190
fragaria_vesca FvH4_2g40810
malus_domestica MD08G1060000.v1.1 MD15G1036500.v1.1
prunus_persica Prupe.1G390800_v2.0.a1
pyrus_communis pycom08g05000 pycom15g03390
rosa_chinensis RchiOBHm_Chr6g0299771
rosa_laevigata RLG00000011390
rosa_multiflora Rmu_co8448481.1_g000001 Rmu_sc0002895.1_g000027
rosa_roxburghii Rroxscaffold_7G00168390
rosa_rugosa Rorug06G0292100
rosa_samantha Rh6AG403100 Rh6BG411600 Rh6CG417700 Rh6DG404500
rosa_wichuraiana Rw6G035280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 165
AcsI RAATTY 1 cut(s) 93
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 1 cut(s) 33
AfiI CCNNNNNNNGG 1 cut(s) 455
AgsI TTSAA 2 cut(s) 386, 518
AjnI CCWGG 2 cut(s) 152, 176
AluBI AGCT 5 cut(s) 149, 229, 328, 416, 574
AluI AGCT 5 cut(s) 149, 229, 328, 416, 574
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 3 cut(s) 64, 107, 213
Ama87I CYCGRG 1 cut(s) 132
AoxI GGCC 1 cut(s) 609
ApeKI GCWGC 3 cut(s) 128, 149, 310
ApoI RAATTY 1 cut(s) 93
AspS9I GGNCC 3 cut(s) 167, 180, 564
AsuC2I CCSGG 2 cut(s) 133, 134
AvaI CYCGRG 1 cut(s) 132
AvaII GGWCC 3 cut(s) 167, 180, 564
AxyI CCTNAGG 1 cut(s) 606
BbsI GAAGAC 1 cut(s) 129
Bbv12I GWGCWC 1 cut(s) 488
BbvI GCAGC 3 cut(s) 136, 140, 322
BceAI ACGGC 2 cut(s) 132, 264
BciT130I CCWGG 2 cut(s) 154, 178
BcnI CCSGG 2 cut(s) 133, 134
BcoDI GTCTC 3 cut(s) 64, 107, 213
BfaI CTAG 2 cut(s) 90, 171
BisI GCNGC 3 cut(s) 129, 150, 311
BlpI GCTNAGC 1 cut(s) 417
BlsI GCNGC 3 cut(s) 130, 151, 312
Bme1390I CCNGG 4 cut(s) 133, 134, 154, 178
Bme18I GGWCC 3 cut(s) 167, 180, 564
BmeT110I CYCGRG 1 cut(s) 132
BmgT120I GGNCC 3 cut(s) 167, 180, 564
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 4 cut(s) 133, 134, 154, 178
BpiI GAAGAC 1 cut(s) 129
BplI GAGNNNNNCTC 2 cut(s) 563, 595
Bpu1102I GCTNAGC 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 133, 134
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 2 cut(s) 25, 132
Bsc4I CCNNNNNNNGG 1 cut(s) 455
Bse21I CCTNAGG 1 cut(s) 606
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 2 cut(s) 154, 178
BseDI CCNNGG 2 cut(s) 25, 132
BseLI CCNNNNNNNGG 1 cut(s) 455
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 5 cut(s) 54, 224, 354, 408, 620
BseRI GAGGAG 1 cut(s) 560
BseXI GCAGC 3 cut(s) 136, 140, 322
BseYI CCCAGC 4 cut(s) 12, 314, 324, 377
BshFI GGCC 1 cut(s) 611
BsiHKAI GWGCWC 1 cut(s) 488
BsiHKCI CYCGRG 1 cut(s) 132
BsiSI CCGG 1 cut(s) 133
BsiWI CGTACG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 455
BsmAI GTCTC 3 cut(s) 64, 107, 213
BsmBI CGTCTC 2 cut(s) 107, 213
BsnI GGCC 1 cut(s) 611
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 488
Bsp1720I GCTNAGC 1 cut(s) 417
BspACI CCGC 1 cut(s) 165
BspANI GGCC 1 cut(s) 611
BspCNI CTCAG 5 cut(s) 55, 223, 355, 409, 619
BspLI GGNNCC 1 cut(s) 565
BspTNI GGTCTC 1 cut(s) 64
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 2 cut(s) 25, 132
Bst2UI CCWGG 2 cut(s) 154, 178
BstC8I GCNNGC 1 cut(s) 320
BstDEI CTNAG 5 cut(s) 63, 210, 363, 417, 606
BstMAI GTCTC 3 cut(s) 64, 107, 213
BstMWI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
BstNI CCWGG 2 cut(s) 154, 178
BstSCI CCNGG 4 cut(s) 131, 132, 152, 176
BstV1I GCAGC 3 cut(s) 136, 140, 322
BstV2I GAAGAC 1 cut(s) 129
Bsu36I CCTNAGG 1 cut(s) 606
BsuRI GGCC 1 cut(s) 611
Cac8I GCNNGC 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 167, 180, 564
Cfr9I CCCGGG 1 cut(s) 132
CseI GACGC 2 cut(s) 134, 195
Csp6I GTAC 1 cut(s) 32
CviAII CATG 1 cut(s) 271
CviQI GTAC 1 cut(s) 32
DdeI CTNAG 5 cut(s) 63, 210, 363, 417, 606
Eco147I AGGCCT 1 cut(s) 611
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 3 cut(s) 167, 180, 564
Eco57I CTGAAG 1 cut(s) 141
Eco81I CCTNAGG 1 cut(s) 606
Eco88I CYCGRG 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 152, 176
Esp3I CGTCTC 2 cut(s) 107, 213
FaeI CATG 1 cut(s) 274
FaiI YATR 7 cut(s) 141, 161, 272, 336, 506, 561, 577
FatI CATG 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 129, 150, 311
Fsp4HI GCNGC 3 cut(s) 129, 150, 311
FspBI CTAG 2 cut(s) 90, 171
GluI GCNGC 3 cut(s) 129, 150, 311
GsaI CCCAGC 4 cut(s) 16, 318, 328, 381
HaeIII GGCC 1 cut(s) 611
HapII CCGG 1 cut(s) 133
HgaI GACGC 2 cut(s) 134, 195
Hin1II CATG 1 cut(s) 274
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HindIII AAGCTT 1 cut(s) 227
HinfI GANTC 2 cut(s) 67, 500
HpaII CCGG 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 340
Hpy188I TCNGA 1 cut(s) 64
Hpy188III TCNNGA 1 cut(s) 515
Hpy8I GTNNAC 1 cut(s) 340
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
HpyF3I CTNAG 5 cut(s) 63, 210, 363, 417, 606
Hsp92II CATG 1 cut(s) 274
LmnI GCTCC 1 cut(s) 558
Lsp1109I GCAGC 3 cut(s) 136, 140, 322
MaeI CTAG 2 cut(s) 90, 171
MaeIII GTNAC 1 cut(s) 586
MboII GAAGA 5 cut(s) 134, 409, 443, 509, 530
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 3 cut(s) 93, 451, 618
MnlI CCTC 7 cut(s) 20, 226, 232, 522, 538, 615, 622
MseI TTAA 1 cut(s) 622
MslI CAYNNNNRTG 1 cut(s) 386
MspA1I CMGCKG 2 cut(s) 328, 416
MspI CCGG 1 cut(s) 133
MspR9I CCNGG 4 cut(s) 133, 134, 154, 178
MvaI CCWGG 2 cut(s) 154, 178
MwoI GCNNNNNNNGC 4 cut(s) 125, 134, 319, 413
NciI CCSGG 2 cut(s) 133, 134
NlaIII CATG 1 cut(s) 274
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 586
PceI AGGCCT 1 cut(s) 611
PfeI GAWTC 2 cut(s) 67, 500
Pfl23II CGTACG 1 cut(s) 31
PkrI GCNGC 3 cut(s) 130, 151, 312
Psp6I CCWGG 2 cut(s) 152, 176
PspFI CCCAGC 4 cut(s) 12, 314, 324, 377
PspGI CCWGG 2 cut(s) 152, 176
PspLI CGTACG 1 cut(s) 31
PspN4I GGNNCC 1 cut(s) 565
PspPI GGNCC 3 cut(s) 167, 180, 564
PvuII CAGCTG 2 cut(s) 328, 416
RsaI GTAC 1 cut(s) 33
RsaNI GTAC 1 cut(s) 32
RseI CAYNNNNRTG 1 cut(s) 386
SaqAI TTAA 1 cut(s) 622
SatI GCNGC 3 cut(s) 129, 150, 311
Sau96I GGNCC 3 cut(s) 167, 180, 564
ScrFI CCNGG 4 cut(s) 133, 134, 154, 178
SduI GDGCHC 1 cut(s) 488
SetI ASST 8 cut(s) 151, 172, 231, 266, 330, 418, 576, 607
SinI GGWCC 3 cut(s) 167, 180, 564
SmaI CCCGGG 1 cut(s) 134
SmiMI CAYNNNNRTG 1 cut(s) 386
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
SrfI GCCCGGGC 1 cut(s) 134
Sse9I AATT 3 cut(s) 93, 451, 618
SseBI AGGCCT 1 cut(s) 611
SsiI CCGC 1 cut(s) 165
SspMI CTAG 2 cut(s) 90, 171
StuI AGGCCT 1 cut(s) 611
StyD4I CCNGG 4 cut(s) 131, 132, 152, 176
TasI AATT 3 cut(s) 93, 451, 618
TfiI GAWTC 2 cut(s) 67, 500
Tru1I TTAA 1 cut(s) 622
Tru9I TTAA 1 cut(s) 622
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 3 cut(s) 128, 149, 310
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 2 cut(s) 159, 510
TspMI CCCGGG 1 cut(s) 132
VpaK11BI GGWCC 3 cut(s) 167, 180, 564
XapI RAATTY 1 cut(s) 93
XmaI CCCGGG 1 cut(s) 132
XspI CTAG 2 cut(s) 90, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.