MD15G1044300.v1.1

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017644
Physical Location & Seq
Forward (+)
3047030 .. 3049852
2823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1044300.v1.1.491

Sequence Viewer

Length: 1533 bp
ATGGAAATTGCTCCGATGAATTTCAGCTCCTTTTTTTTCAGGTCTTTTGGTTTATATGTATTATTTATATGTCTAGTTCTCTGCTCTTTGGAGAAGGGTTTTGCCTTGGCAGGAAGAAAGGATAAACTTACTCATCACACAGTTCAGTTGAACTCTCTACTGCCAGCAACTACTTGCACCCCCTCCACCAAAGGTCACAACAAGAAACATGGATCAGTACTAGAAGTGGTACACAGGCATGGCCCTTGCTACGAACCCAACCAACACAAAACCAAAACCGCTACAGATCTTCACGAATATTTCACTCAATTCTTCAAGCAAGACCAAGCACGAGTCGATTCAATCTACTCCCGCCTAAAGGCCACCAAAAGATATTCAACCAAAACAGATGACAACATTCCGATTACGCAATCGGAAGATACCAACACCTTCCCGGCCCCTTCTGCCAGGACGGTGGGTGGTTCTGGCAACTACGTTGTGAAGGTCGGATTAGGCACGCCGGCCAAAAGTTTTTCCCTCGTATTCGACACCGGAAGCGACCTAACTTGGACTCAATGCCAGCCTTGTCGATTCGGCACCGGAAGCGACCTAACCTGTTACGACCAAACGGAGCCCATATATGACCCGTCACTCTCCACCTCCTACGCCAACATCTCATGCAACACCGCAACTTGCAATGAGCTGACGTCGGCCACAAGCACTGGACCCTACTGTTACCGGGCGACGAACACGTGCCTGTACCTCATTGGGTACGGAGACAACTCCACATCCGCCGGATATTACGGAACCGAAAGGCTCACGTTGACCGCCACTTCGACCGACGTGTTTGATGGCTTCCTCTTCGGGTGCGGCCAAGACAACGAAGGTCTCTTTGTTGGCATTGCCGGTTTGCTTGGTCTCGGCCGGAACAAGATATCCCTCATCGAACAAACCGCCGCAAAGTACGGCCGATATTTCTCCTACTGCCTTCCTGTCGACCAGAGCTCCACAGGCTTTCTCCGCCTCGGCAACGACCGAGGAAATTCCGCCGCCGTTAAGTTCACGCCGCTCACCACCCTTGCTGCAGGAGAATCATTTTACGGGCTCGATTTGGTCGGCATCAGTGTCAGCGGACAACAGGTGTCCATTCCGTCGACGGTTTTTTCCACCGGAACTCTCATCGACTCGGGGACAGTGATAACGCGGCTGCCGGCAGTGGCGTACACCGCGATGAGAGACGCTTTTAGGGAAGGGATGAAGAGCTATGGGGAACCCAAGACAGTTGAGGGGGTACTGGACACTTGCTACGACCTTAGAGCGTATAACTTGGATACGGTCTCGTTTCCGGCGGTAGCGTTTACGTTCAGTGGCGGGCTTACTTTGGAATTGAAAGTGGGGGGGACAGTGGTTGTGATAGTTGATACTTCGCAGGTTTGCTTGGCGTTTCTTCCTACCCCCGAAGAGGGGATCATTGGGAATTTTCAGCAGAAAGGGTTTGAAGTGGTGTACGATGTTGCAGGAGGGAAGATTGGATTCGCCACTGGAAGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

511

Amino Acids

54.99

Weight (kDa)

5.94

Isoelectric Point (pI)

27.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 157 - 507 1.3e-16 Eukaryotic aspartyl protease
TAXi_N PF14543 158 - 337 1e-50 Xylanase inhibitor N-terminal
TAXi_C PF14541 360 - 506 3.7e-25 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08261
malus_domestica MD15G1044300.v1.1
pyrus_communis pycom15g04120
rosa_laevigata RLG00000009074 RLG00000009077
rosa_samantha Rh4AG113400 Rh4AG113700 Rh4AG113900 Rh4BG106100 Rh4CG120500 Rh4DG105400 Rh6CG035400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 689
Acc36I ACCTGC 1 cut(s) 1399
AccB1I GGYRCC 1 cut(s) 575
AccBSI CCGCTC 1 cut(s) 1048
AccI GTMKAC 2 cut(s) 975, 1133
AccII CGCG 2 cut(s) 1183, 1208
AclWI GGATC 2 cut(s) 220, 1454
AcoI YGGCCR 5 cut(s) 501, 690, 850, 901, 946
AcsI RAATTY 3 cut(s) 19, 1021, 1456
AcvI CACGTG 1 cut(s) 732
AcyI GRCGYC 1 cut(s) 686
AfaI GTAC 8 cut(s) 219, 231, 740, 752, 944, 1202, 1272, 1487
AfiI CCNNNNNNNGG 4 cut(s) 358, 1441, 1442, 1443
AflIII ACRYGT 2 cut(s) 729, 822
AgsI TTSAA 6 cut(s) 151, 316, 342, 378, 1369, 1478
AjiI CACGTC 1 cut(s) 823
AjnI CCWGG 1 cut(s) 446
AloI GAACNNNNNNTCC 2 cut(s) 899, 931
AluBI AGCT 4 cut(s) 27, 682, 984, 1242
AluI AGCT 4 cut(s) 27, 682, 984, 1242
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 5 cut(s) 750, 872, 902, 1209, 1321
AlwI GGATC 2 cut(s) 220, 1454
Ama87I CYCGRG 1 cut(s) 1165
AoxI GGCC 8 cut(s) 241, 360, 435, 501, 690, 850, 901, 946
ApeKI GCWGC 2 cut(s) 1061, 1186
ApoI RAATTY 3 cut(s) 19, 1021, 1456
ArsI GACNNNNNNTTYG 2 cut(s) 796, 828
AspS9I GGNCC 3 cut(s) 242, 436, 704
AsuC2I CCSGG 2 cut(s) 434, 719
AsuHPI GGTGA 1 cut(s) 1042
AvaI CYCGRG 1 cut(s) 1165
AvaII GGWCC 1 cut(s) 704
BanI GGYRCC 1 cut(s) 575
BanII GRGCYC 3 cut(s) 615, 986, 1086
BauI CACGAG 1 cut(s) 330
BbrPI CACGTG 1 cut(s) 732
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 2 cut(s) 1048, 1173
BccI CCATC 1 cut(s) 824
BceAI ACGGC 2 cut(s) 961, 1016
BciT130I CCWGG 1 cut(s) 448
BciVI GTATCC 1 cut(s) 1303
BcnI CCSGG 2 cut(s) 434, 719
BcoDI GTCTC 5 cut(s) 750, 872, 902, 1209, 1321
BfaI CTAG 2 cut(s) 74, 221
BfmI CTRYAG 2 cut(s) 282, 1062
BfuAI ACCTGC 1 cut(s) 1399
BfuI GTATCC 1 cut(s) 1303
BglII AGATCT 1 cut(s) 286
BisI GCNGC 7 cut(s) 850, 936, 1029, 1046, 1062, 1184, 1187
BlsI GCNGC 7 cut(s) 851, 937, 1030, 1047, 1063, 1185, 1188
BmcAI AGTACT 1 cut(s) 219
Bme1390I CCNGG 3 cut(s) 434, 448, 719
Bme18I GGWCC 1 cut(s) 704
BmeT110I CYCGRG 1 cut(s) 1165
BmgBI CACGTC 1 cut(s) 823
BmgT120I GGNCC 3 cut(s) 242, 436, 704
BmiI GGNNCC 6 cut(s) 438, 577, 612, 706, 787, 1251
BmrFI CCNGG 3 cut(s) 434, 448, 719
BmsI GCATC 1 cut(s) 1107
BpuMI CCSGG 2 cut(s) 434, 719
BsaAI YACGTR 1 cut(s) 732
BsaHI GRCGYC 1 cut(s) 686
BsaI GGTCTC 3 cut(s) 872, 902, 1321
BsaJI CCNNGG 3 cut(s) 105, 1003, 1015
BsaWI WCCGGW 3 cut(s) 530, 578, 1148
BsaXI ACNNNNNCTCC 2 cut(s) 968, 998
Bsc4I CCNNNNNNNGG 4 cut(s) 358, 1441, 1442, 1443
Bse118I RCCGGY 3 cut(s) 499, 884, 1189
Bse1I ACTGG 3 cut(s) 706, 1278, 1525
Bse3DI GCAATG 2 cut(s) 682, 879
BseBI CCWGG 1 cut(s) 448
BseDI CCNNGG 3 cut(s) 105, 1003, 1015
BseGI GGATG 2 cut(s) 767, 1239
BseLI CCNNNNNNNGG 4 cut(s) 358, 1441, 1442, 1443
BseMI GCAATG 2 cut(s) 682, 879
BseNI ACTGG 3 cut(s) 706, 1278, 1525
BseX3I CGGCCG 2 cut(s) 901, 946
BseXI GCAGC 2 cut(s) 1048, 1173
Bsh1236I CGCG 2 cut(s) 1183, 1208
Bsh1285I CGRYCG 4 cut(s) 819, 904, 949, 1015
BshFI GGCC 8 cut(s) 243, 362, 437, 503, 692, 852, 903, 948
BshNI GGYRCC 1 cut(s) 575
BsiEI CGRYCG 4 cut(s) 819, 904, 949, 1015
BsiHKAI GWGCWC 1 cut(s) 986
BsiHKCI CYCGRG 1 cut(s) 1165
BslFI GGGAC 2 cut(s) 1183, 1393
BslI CCNNNNNNNGG 4 cut(s) 358, 1441, 1442, 1443
BsmAI GTCTC 5 cut(s) 750, 872, 902, 1209, 1321
BsmBI CGTCTC 1 cut(s) 1209
BsmFI GGGAC 2 cut(s) 1183, 1393
BsnI GGCC 8 cut(s) 243, 362, 437, 503, 692, 852, 903, 948
Bso31I GGTCTC 3 cut(s) 872, 902, 1321
BsoBI CYCGRG 1 cut(s) 1165
Bsp1286I GDGCHC 3 cut(s) 615, 986, 1086
Bsp143I GATC 3 cut(s) 212, 286, 1446
BspANI GGCC 8 cut(s) 243, 362, 437, 503, 692, 852, 903, 948
BspFNI CGCG 2 cut(s) 1183, 1208
BspLI GGNNCC 6 cut(s) 438, 577, 612, 706, 787, 1251
BspMAI CTGCAG 1 cut(s) 1066
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 2 cut(s) 220, 1454
BspQI GCTCTTC 1 cut(s) 1232
BspT107I GGYRCC 1 cut(s) 575
BspTNI GGTCTC 3 cut(s) 872, 902, 1321
BsrBI CCGCTC 1 cut(s) 1048
BsrDI GCAATG 2 cut(s) 682, 879
BsrFI RCCGGY 3 cut(s) 499, 884, 1189
BsrI ACTGG 3 cut(s) 706, 1278, 1525
BssAI RCCGGY 3 cut(s) 499, 884, 1189
BssECI CCNNGG 3 cut(s) 105, 1003, 1015
BssMI GATC 3 cut(s) 212, 286, 1446
BssNI GRCGYC 1 cut(s) 686
BssSI CACGAG 1 cut(s) 330
BssT1I CCWWGG 1 cut(s) 105
Bst2BI CACGAG 1 cut(s) 330
Bst2UI CCWGG 1 cut(s) 448
Bst4CI ACNGT 8 cut(s) 142, 454, 713, 1138, 1174, 1261, 1315, 1384
Bst6I CTCTTC 3 cut(s) 845, 1232, 1434
BstACI GRCGYC 1 cut(s) 686
BstBAI YACGTR 1 cut(s) 732
BstC8I GCNNGC 6 cut(s) 165, 497, 501, 560, 1191, 1352
BstDEI CTNAG 1 cut(s) 1292
BstF5I GGATG 2 cut(s) 767, 1239
BstFNI CGCG 2 cut(s) 1183, 1208
BstKTI GATC 3 cut(s) 215, 289, 1449
BstMAI GTCTC 5 cut(s) 750, 872, 902, 1209, 1321
BstMBI GATC 3 cut(s) 212, 286, 1446
BstMCI CGRYCG 4 cut(s) 819, 904, 949, 1015
BstMWI GCNNNNNNNGC 5 cut(s) 443, 582, 990, 999, 1205
BstNI CCWGG 1 cut(s) 448
BstSCI CCNGG 3 cut(s) 432, 446, 717
BstSFI CTRYAG 2 cut(s) 282, 1062
BstUI CGCG 2 cut(s) 1183, 1208
BstV1I GCAGC 2 cut(s) 1048, 1173
BstX2I RGATCY 1 cut(s) 286
BstXI CCANNNNNNTGG 1 cut(s) 454
BstYI RGATCY 1 cut(s) 286
BstZI CGGCCG 2 cut(s) 901, 946
BsuI GTATCC 1 cut(s) 1303
BsuRI GGCC 8 cut(s) 243, 362, 437, 503, 692, 852, 903, 948
BtgZI GCGATG 1 cut(s) 1223
BtrI CACGTC 1 cut(s) 823
BtsCI GGATG 2 cut(s) 767, 1239
BtsI GCAGTG 1 cut(s) 1200
BtsIMutI CAGTG 7 cut(s) 699, 1108, 1179, 1200, 1351, 1389, 1518
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 6 cut(s) 165, 497, 501, 560, 1191, 1352
Cfr10I RCCGGY 3 cut(s) 499, 884, 1189
Cfr13I GGNCC 3 cut(s) 242, 436, 704
CseI GACGC 1 cut(s) 1226
Csp6I GTAC 8 cut(s) 218, 230, 739, 751, 943, 1201, 1271, 1486
CviAII CATG 3 cut(s) 209, 239, 657
CviQI GTAC 8 cut(s) 218, 230, 739, 751, 943, 1201, 1271, 1486
DdeI CTNAG 1 cut(s) 1292
DpnI GATC 3 cut(s) 214, 288, 1448
DpnII GATC 3 cut(s) 212, 286, 1446
EaeI YGGCCR 5 cut(s) 501, 690, 850, 901, 946
EagI CGGCCG 2 cut(s) 901, 946
Eam1104I CTCTTC 3 cut(s) 845, 1232, 1434
EarI CTCTTC 3 cut(s) 845, 1232, 1434
EciI GGCGGA 3 cut(s) 760, 989, 1015
Ecl136II GAGCTC 1 cut(s) 984
EclXI CGGCCG 2 cut(s) 901, 946
Eco130I CCWWGG 1 cut(s) 105
Eco24I GRGCYC 3 cut(s) 615, 986, 1086
Eco31I GGTCTC 3 cut(s) 872, 902, 1321
Eco32I GATATC 1 cut(s) 915
Eco47I GGWCC 1 cut(s) 704
Eco52I CGGCCG 2 cut(s) 901, 946
Eco53kI GAGCTC 1 cut(s) 984
Eco72I CACGTG 1 cut(s) 732
Eco88I CYCGRG 1 cut(s) 1165
EcoICRI GAGCTC 1 cut(s) 984
EcoRII CCWGG 1 cut(s) 446
EcoRV GATATC 1 cut(s) 915
EcoT14I CCWWGG 1 cut(s) 105
EcoT38I GRGCYC 3 cut(s) 615, 986, 1086
ErhI CCWWGG 1 cut(s) 105
Esp3I CGTCTC 1 cut(s) 1209
FaeI CATG 3 cut(s) 212, 242, 660
FaqI GGGAC 2 cut(s) 1183, 1393
FatI CATG 3 cut(s) 208, 238, 656
FauI CCCGC 2 cut(s) 359, 1343
FblI GTMKAC 2 cut(s) 975, 1133
Fnu4HI GCNGC 7 cut(s) 850, 936, 1029, 1046, 1062, 1184, 1187
FokI GGATG 2 cut(s) 754, 1246
FriOI GRGCYC 3 cut(s) 615, 986, 1086
Fsp4HI GCNGC 7 cut(s) 850, 936, 1029, 1046, 1062, 1184, 1187
FspBI CTAG 2 cut(s) 74, 221
GluI GCNGC 7 cut(s) 850, 936, 1029, 1046, 1062, 1184, 1187
HaeIII GGCC 8 cut(s) 243, 362, 437, 503, 692, 852, 903, 948
HgaI GACGC 1 cut(s) 1226
Hin1I GRCGYC 1 cut(s) 686
Hin1II CATG 3 cut(s) 212, 242, 660
HincII GTYRAC 3 cut(s) 804, 976, 1134
HindII GTYRAC 3 cut(s) 804, 976, 1134
HinfI GANTC 7 cut(s) 333, 338, 550, 570, 1070, 1163, 1512
HphI GGTGA 1 cut(s) 1042
Hpy166II GTNNAC 8 cut(s) 232, 804, 976, 1041, 1134, 1203, 1338, 1486
Hpy188I TCNGA 4 cut(s) 15, 402, 415, 488
Hpy188III TCNNGA 1 cut(s) 293
Hpy8I GTNNAC 8 cut(s) 232, 804, 976, 1041, 1134, 1203, 1338, 1486
Hpy99I CGWCG 5 cut(s) 691, 727, 824, 1135, 1138
HpyAV CCTTC 7 cut(s) 88, 439, 450, 475, 857, 977, 1223
HpyCH4III ACNGT 8 cut(s) 142, 454, 713, 1138, 1174, 1261, 1315, 1384
HpyCH4IV ACGT 6 cut(s) 474, 686, 731, 800, 822, 1340
HpyCH4V TGCA 5 cut(s) 177, 660, 675, 1064, 1496
HpyF10VI GCNNNNNNNGC 5 cut(s) 443, 582, 990, 999, 1205
HpyF3I CTNAG 1 cut(s) 1292
HpySE526I ACGT 6 cut(s) 474, 686, 731, 800, 822, 1340
Hsp92I GRCGYC 1 cut(s) 686
Hsp92II CATG 3 cut(s) 212, 242, 660
KroI GCCGGC 2 cut(s) 499, 1189
KroNI GCCGGC 2 cut(s) 501, 1191
Kzo9I GATC 3 cut(s) 212, 286, 1446
LguI GCTCTTC 1 cut(s) 1232
LmnI GCTCC 4 cut(s) 16, 32, 610, 989
Lsp1109I GCAGC 2 cut(s) 1048, 1173
LweI GCATC 1 cut(s) 1107
MaeI CTAG 2 cut(s) 74, 221
MaeII ACGT 6 cut(s) 474, 686, 731, 800, 822, 1340
MaeIII GTNAC 4 cut(s) 194, 596, 627, 713
MalI GATC 3 cut(s) 214, 288, 1448
MbiI CCGCTC 1 cut(s) 1048
MboI GATC 3 cut(s) 212, 286, 1446
MboII GAAGA 9 cut(s) 126, 281, 304, 428, 832, 1249, 1418, 1451, 1516
MflI RGATCY 1 cut(s) 286
MhlI GDGCHC 3 cut(s) 615, 986, 1086
MluCI AATT 6 cut(s) 6, 19, 308, 1021, 1364, 1456
MlyI GAGTC 3 cut(s) 342, 544, 1157
MmeI TCCRAC 1 cut(s) 466
MroNI GCCGGC 2 cut(s) 499, 1189
MseI TTAA 1 cut(s) 1035
MslI CAYNNNNRTG 2 cut(s) 237, 1208
MspA1I CMGCKG 1 cut(s) 1110
MspR9I CCNGG 3 cut(s) 434, 448, 719
MvaI CCWGG 1 cut(s) 448
MvnI CGCG 2 cut(s) 1183, 1208
MwoI GCNNNNNNNGC 5 cut(s) 443, 582, 990, 999, 1205
NaeI GCCGGC 2 cut(s) 501, 1191
NciI CCSGG 2 cut(s) 434, 719
NdeII GATC 3 cut(s) 212, 286, 1446
NgoMIV GCCGGC 2 cut(s) 499, 1189
NlaIII CATG 3 cut(s) 212, 242, 660
NlaIV GGNNCC 6 cut(s) 438, 577, 612, 706, 787, 1251
NmeAIII GCCGAG 2 cut(s) 879, 984
NmuCI GTSAC 2 cut(s) 194, 627
PciSI GCTCTTC 1 cut(s) 1232
PdiI GCCGGC 2 cut(s) 501, 1191
PfeI GAWTC 4 cut(s) 338, 570, 1070, 1512
PkrI GCNGC 7 cut(s) 851, 937, 1030, 1047, 1063, 1185, 1188
PleI GAGTC 3 cut(s) 341, 544, 1157
PmaCI CACGTG 1 cut(s) 732
PmlI CACGTG 1 cut(s) 732
PpsI GAGTC 3 cut(s) 341, 544, 1157
Ppu21I YACGTR 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 986
Psp6I CCWGG 1 cut(s) 446
PspCI CACGTG 1 cut(s) 732
PspGI CCWGG 1 cut(s) 446
PspN4I GGNNCC 6 cut(s) 438, 577, 612, 706, 787, 1251
PspPI GGNCC 3 cut(s) 242, 436, 704
PstI CTGCAG 1 cut(s) 1066
PsuI RGATCY 1 cut(s) 286
RsaI GTAC 8 cut(s) 219, 231, 740, 752, 944, 1202, 1272, 1487
RsaNI GTAC 8 cut(s) 218, 230, 739, 751, 943, 1201, 1271, 1486
RseI CAYNNNNRTG 2 cut(s) 237, 1208
SacI GAGCTC 1 cut(s) 986
SalI GTCGAC 2 cut(s) 974, 1132
SapI GCTCTTC 1 cut(s) 1232
SaqAI TTAA 1 cut(s) 1035
SatI GCNGC 7 cut(s) 850, 936, 1029, 1046, 1062, 1184, 1187
Sau3AI GATC 3 cut(s) 212, 286, 1446
Sau96I GGNCC 3 cut(s) 242, 436, 704
ScaI AGTACT 1 cut(s) 219
SchI GAGTC 3 cut(s) 342, 544, 1157
ScrFI CCNGG 3 cut(s) 434, 448, 719
SduI GDGCHC 3 cut(s) 615, 986, 1086
SfaNI GCATC 1 cut(s) 1107
SfcI CTRYAG 2 cut(s) 282, 1062
SgrDI CGTCGACG 1 cut(s) 1132
SinI GGWCC 1 cut(s) 704
SmiMI CAYNNNNRTG 2 cut(s) 237, 1208
Sse9I AATT 6 cut(s) 6, 19, 308, 1021, 1364, 1456
SspI AATATT 1 cut(s) 299
SspMI CTAG 2 cut(s) 74, 221
SstI GAGCTC 1 cut(s) 986
StyD4I CCNGG 3 cut(s) 432, 446, 717
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 8 cut(s) 142, 454, 713, 1138, 1174, 1261, 1315, 1384
TaiI ACGT 6 cut(s) 477, 689, 734, 803, 825, 1343
TaqI TCGA 9 cut(s) 336, 525, 568, 815, 924, 975, 1086, 1133, 1161
TaqII GACCGA 2 cut(s) 833, 1029
TasI AATT 6 cut(s) 6, 19, 308, 1021, 1364, 1456
TatI WGTACW 1 cut(s) 217
TauI GCSGC 5 cut(s) 852, 938, 1031, 1048, 1186
TfiI GAWTC 4 cut(s) 338, 570, 1070, 1512
Tru1I TTAA 1 cut(s) 1035
Tru9I TTAA 1 cut(s) 1035
TscAI CASTG 7 cut(s) 706, 1108, 1179, 1200, 1351, 1389, 1525
TseFI GTSAC 2 cut(s) 194, 627
TseI GCWGC 2 cut(s) 1061, 1186
Tsp45I GTSAC 2 cut(s) 194, 627
TspDTI ATGAA 2 cut(s) 32, 1250
TspGWI ACGGA 4 cut(s) 623, 768, 798, 1119
TspRI CASTG 7 cut(s) 706, 1108, 1179, 1200, 1351, 1389, 1525
VpaK11BI GGWCC 1 cut(s) 704
XapI RAATTY 3 cut(s) 19, 1021, 1456
XmiI GTMKAC 2 cut(s) 975, 1133
XspI CTAG 2 cut(s) 74, 221
ZraI GACGTC 1 cut(s) 687
ZrmI AGTACT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.