Rh4AG113400

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017644
Physical Location & Seq
Forward (+)
24525637 .. 24559403
33767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG113400.1

Sequence Viewer

Length: 630 bp
ATGGCATCTCTTGAAGTGGTACACAAGCATGGGCCATGCTCTCATCTTCCTCAACTCAAAGCGACTGCTATGAATCACACTCTAATTCTCGAGCAAGACCAAGCGCGAGTCAACTCAATCAACTCCCGGATTTCCAAGAAATTGAACGGCAATGAGGATCTAAGCCAATCGGAGGCCACCACCGTTCCGGCCCGGTGGGCAAGCAGCCAAAATGCAGGCAACTACATGGTGACGGTGGGATTGGGCACACCTAAAAAACAACTTTCGCTCGTATTTGACACCGGAAGCGACCTCACTTGGACTCAATGCCAACCTTGTTCTGAATTTTGCTACCCACAAGAGGAGCCCATTTTCGATCCTTCGGCCTCTTCCTCATATACCAACGTTTCCTGTAACAGCACAGAGTGCGTCCAACTCAGGACATCTACGGATCGTAACGATAAGTTGTATGAGGGTTTCGAACTGCTCATTTGGGATTTACTCAACAACAGATTATATATGGTTGGAATTCCGAAAACGGATGTTGTCATTACTATGAGATTAAAAGAGTGGCAGAGTCAGAAAAATGGATGGATTTTTCTCCAAAAATGTTTCTGGAAGTTTGCATATTTTATGGATGTGACTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152
Pfam Domains
Protein Families
TAXi_C TAXi_N

Protein Analysis

209

Amino Acids

23.72

Weight (kDa)

6.08

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asp PF00026 74 - 129 1.8e-09 Eukaryotic aspartyl protease
TAXi_N PF14543 75 - 141 3.9e-23 Xylanase inhibitor N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017644)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g08261
malus_domestica MD15G1044300.v1.1
pyrus_communis pycom15g04120
rosa_laevigata RLG00000009074 RLG00000009077
rosa_samantha Rh4AG113400 Rh4AG113700 Rh4AG113900 Rh4BG106100 Rh4CG120500 Rh4DG105400 Rh6CG035400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 106
AclI AACGTT 1 cut(s) 384
AclWI GGATC 3 cut(s) 165, 350, 438
AcsI RAATTY 2 cut(s) 323, 507
AdeI CACNNNGTG 1 cut(s) 405
AfaI GTAC 1 cut(s) 21
AfiI CCNNNNNNNGG 2 cut(s) 172, 340
AgsI TTSAA 2 cut(s) 14, 145
AlwI GGATC 3 cut(s) 165, 350, 438
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 4 cut(s) 32, 174, 189, 363
ApeKI GCWGC 1 cut(s) 204
ApoI RAATTY 2 cut(s) 323, 507
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 2 cut(s) 32, 190
AsuC2I CCSGG 2 cut(s) 127, 193
AsuHPI GGTGA 1 cut(s) 241
AsuII TTCGAA 1 cut(s) 459
AvaI CYCGRG 1 cut(s) 89
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 216
BccI CCATC 1 cut(s) 564
BceAI ACGGC 1 cut(s) 163
BcnI CCSGG 2 cut(s) 127, 193
BfaI CTAG 1 cut(s) 628
BglI GCCNNNNNGGC 1 cut(s) 197
BisI GCNGC 1 cut(s) 205
BlsI GCNGC 1 cut(s) 206
Bme1390I CCNGG 2 cut(s) 127, 193
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 32, 190
BmiI GGNNCC 1 cut(s) 345
BmrFI CCNGG 2 cut(s) 127, 193
BmsI GCATC 1 cut(s) 14
Bpu14I TTCGAA 1 cut(s) 459
BpuMI CCSGG 2 cut(s) 127, 193
BsaWI WCCGGW 1 cut(s) 281
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 340
Bse3DI GCAATG 1 cut(s) 157
BseGI GGATG 3 cut(s) 526, 575, 622
BseLI CCNNNNNNNGG 2 cut(s) 172, 340
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 1 cut(s) 216
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 4 cut(s) 34, 176, 191, 365
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 4 cut(s) 127, 188, 193, 282
BslI CCNNNNNNNGG 2 cut(s) 172, 340
BsnI GGCC 4 cut(s) 34, 176, 191, 365
BsoBI CYCGRG 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 459
Bsp1286I GDGCHC 2 cut(s) 248, 348
Bsp143I GATC 3 cut(s) 157, 355, 430
BspANI GGCC 4 cut(s) 34, 176, 191, 365
BspCNI CTCAG 1 cut(s) 429
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 345
BspPI GGATC 3 cut(s) 165, 350, 438
BspT104I TTCGAA 1 cut(s) 459
BsrDI GCAATG 1 cut(s) 157
BssMI GATC 3 cut(s) 157, 355, 430
Bst4CI ACNGT 2 cut(s) 184, 235
Bst6I CTCTTC 1 cut(s) 373
BstAPI GCANNNNNTGC 1 cut(s) 405
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 2 cut(s) 202, 217
BstDEI CTNAG 2 cut(s) 161, 416
BstF5I GGATG 3 cut(s) 526, 575, 622
BstFNI CGCG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 3 cut(s) 160, 358, 433
BstMBI GATC 3 cut(s) 157, 355, 430
BstMWI GCNNNNNNNGC 2 cut(s) 197, 405
BstSCI CCNGG 2 cut(s) 125, 191
BstSLI GKGCMC 1 cut(s) 248
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 1 cut(s) 216
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuRI GGCC 4 cut(s) 34, 176, 191, 365
BtsCI GGATG 3 cut(s) 526, 575, 622
Cac8I GCNNGC 2 cut(s) 202, 217
CfoI GCGC 1 cut(s) 106
Cfr13I GGNCC 2 cut(s) 32, 190
CseI GACGC 1 cut(s) 397
Csp6I GTAC 1 cut(s) 20
CviAII CATG 3 cut(s) 29, 36, 226
CviJI RGCY 7 cut(s) 34, 165, 176, 191, 207, 346, 365
CviKI_1 RGCY 7 cut(s) 34, 165, 176, 191, 207, 346, 365
CviQI GTAC 1 cut(s) 20
DdeI CTNAG 2 cut(s) 161, 416
DpnI GATC 3 cut(s) 159, 357, 432
DpnII GATC 3 cut(s) 157, 355, 430
DraIII CACNNNGTG 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 373
EarI CTCTTC 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 348
Eco88I CYCGRG 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 507
EcoT38I GRGCYC 1 cut(s) 348
FaeI CATG 3 cut(s) 32, 39, 229
FatI CATG 3 cut(s) 28, 35, 225
Fnu4HI GCNGC 1 cut(s) 205
FokI GGATG 2 cut(s) 533, 582
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 1 cut(s) 205
FspBI CTAG 1 cut(s) 628
GlaI GCGC 1 cut(s) 105
GluI GCNGC 1 cut(s) 205
HaeIII GGCC 4 cut(s) 34, 176, 191, 365
HapII CCGG 4 cut(s) 127, 188, 193, 282
HgaI GACGC 1 cut(s) 397
HhaI GCGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 32, 39, 229
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HinfI GANTC 4 cut(s) 73, 108, 301, 556
HpaII CCGG 4 cut(s) 127, 188, 193, 282
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 22, 112
Hpy188I TCNGA 4 cut(s) 172, 322, 513, 561
Hpy188III TCNNGA 4 cut(s) 11, 89, 418, 595
Hpy8I GTNNAC 2 cut(s) 22, 112
HpyAV CCTTC 1 cut(s) 369
HpyCH4III ACNGT 2 cut(s) 184, 235
HpyCH4IV ACGT 1 cut(s) 384
HpyCH4V TGCA 2 cut(s) 215, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 197, 405
HpyF3I CTNAG 2 cut(s) 161, 416
HpySE526I ACGT 1 cut(s) 384
Hsp92II CATG 3 cut(s) 32, 39, 229
HspAI GCGC 1 cut(s) 104
Kzo9I GATC 3 cut(s) 157, 355, 430
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 8 cut(s) 140, 201, 201, 206, 295, 403, 403, 580
Lsp1109I GCAGC 1 cut(s) 216
LweI GCATC 1 cut(s) 14
MaeI CTAG 1 cut(s) 628
MaeII ACGT 1 cut(s) 384
MaeIII GTNAC 4 cut(s) 229, 392, 434, 619
MalI GATC 3 cut(s) 159, 357, 432
MboI GATC 3 cut(s) 157, 355, 430
MboII GAAGA 2 cut(s) 38, 360
MflI RGATCY 1 cut(s) 157
MhlI GDGCHC 2 cut(s) 248, 348
MluCI AATT 4 cut(s) 84, 140, 323, 507
MlyI GAGTC 3 cut(s) 117, 295, 565
MmeI TCCRAC 2 cut(s) 436, 484
MnlI CCTC 8 cut(s) 60, 148, 166, 302, 334, 376, 382, 445
MseI TTAA 1 cut(s) 542
MslI CAYNNNNRTG 2 cut(s) 27, 533
MspI CCGG 4 cut(s) 127, 188, 193, 282
MspR9I CCNGG 2 cut(s) 127, 193
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 2 cut(s) 197, 405
NciI CCSGG 2 cut(s) 127, 193
NdeII GATC 3 cut(s) 157, 355, 430
NlaIII CATG 3 cut(s) 32, 39, 229
NlaIV GGNNCC 1 cut(s) 345
NmuCI GTSAC 2 cut(s) 229, 619
NspV TTCGAA 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 89
PfeI GAWTC 1 cut(s) 73
PfoI TCCNGGA 1 cut(s) 125
PkrI GCNGC 1 cut(s) 206
PleI GAGTC 3 cut(s) 116, 295, 564
PpsI GAGTC 3 cut(s) 116, 295, 564
Psp1406I AACGTT 1 cut(s) 384
PspN4I GGNNCC 1 cut(s) 345
PspPI GGNCC 2 cut(s) 32, 190
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 1 cut(s) 21
RsaNI GTAC 1 cut(s) 20
RseI CAYNNNNRTG 2 cut(s) 27, 533
SaqAI TTAA 1 cut(s) 542
SatI GCNGC 1 cut(s) 205
Sau3AI GATC 3 cut(s) 157, 355, 430
Sau96I GGNCC 2 cut(s) 32, 190
SchI GAGTC 3 cut(s) 117, 295, 565
ScrFI CCNGG 2 cut(s) 127, 193
SduI GDGCHC 2 cut(s) 248, 348
SetI ASST 5 cut(s) 253, 294, 316, 387, 629
SfaNI GCATC 1 cut(s) 14
Sfr274I CTCGAG 1 cut(s) 89
SfuI TTCGAA 1 cut(s) 459
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 2 cut(s) 27, 533
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 4 cut(s) 84, 140, 323, 507
SspMI CTAG 1 cut(s) 628
StyD4I CCNGG 2 cut(s) 125, 191
TaaI ACNGT 2 cut(s) 184, 235
TaiI ACGT 1 cut(s) 387
TaqI TCGA 3 cut(s) 90, 354, 459
TasI AATT 4 cut(s) 84, 140, 323, 507
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 1 cut(s) 542
Tru9I TTAA 1 cut(s) 542
TseFI GTSAC 2 cut(s) 229, 619
TseI GCWGC 1 cut(s) 204
Tsp45I GTSAC 2 cut(s) 229, 619
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 2 cut(s) 443, 533
XapI RAATTY 2 cut(s) 323, 507
XhoI CTCGAG 1 cut(s) 89
XspI CTAG 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.