MD15G1045700.v1.1

TAFs are components of the transcription factor IID (TFIID) complex that is essential for mediating regulation of RNA polymerase transcription

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006093
Physical Location & Seq
Forward (+)
3141071 .. 3143297
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1045700.v1.1.491

Sequence Viewer

Length: 402 bp
ATGAACCAGAACCCAAATCAGAACCAGAACCAGCAACCGAGCGACGGCGGAAAGCACGAGGATGACGCCGCCCTCACCGACTTCCTCGCCTCCTTGATGGACTACACCCCCACCATGCCCGACGAGCTCGTGGAGCATTACTTGGCGAAAAGCGGCTTCCAATGCTCCGACGTTCGATTAATTAGGCTGGTAGCTGTCGCCACGCAAAAGTTTGTTTCTGAGGTCGCAACTGATGCTCTTCAGCAATGCAAAGCGAGGCAGGCTTCTATAGATAAGCGTCTAATATTGACAATGGAAGATCTGTCAAGAGCGTTGCGTGAGTATGGCGTTAATGTGAAGCACCAAGAATATTTTGCGGACAGCCCTTCGACTGGGATGGATCCTGCAACACGGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000082 GO:0000123 GO:0000125 GO:0000278 GO:0000428 GO:0001889 GO:0003674 GO:0003676 GO:0003677 GO:0003712 GO:0003713 GO:0005102 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0005669 GO:0005737 GO:0006139 GO:0006325 GO:0006351 GO:0006352 GO:0006355 GO:0006366 GO:0006367 GO:0006464 GO:0006473 GO:0006475 GO:0006508 GO:0006725 GO:0006807 GO:0006915 GO:0006996 GO:0007049 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009059 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0012501 GO:0016043 GO:0016070 GO:0016569 GO:0016570 GO:0016573 GO:0016578 GO:0016579 GO:0016591 GO:0018130 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019899 GO:0022402 GO:0022607 GO:0030154 GO:0030331 GO:0030855 GO:0030880 GO:0030914 GO:0031248 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033276 GO:0034622 GO:0034641 GO:0034645 GO:0034654 GO:0035257 GO:0035258 GO:0035264 GO:0036211 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043933 GO:0043966 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044770 GO:0044772 GO:0044798 GO:0044843 GO:0045935 GO:0046483 GO:0048471 GO:0048513 GO:0048518 GO:0048522 GO:0048589 GO:0048731 GO:0048732 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051098 GO:0051101 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051259 GO:0051260 GO:0051276 GO:0051427 GO:0055029 GO:0060255 GO:0060429 GO:0061008 GO:0061695 GO:0065003 GO:0065007 GO:0065009 GO:0070013 GO:0070063 GO:0070365 GO:0070461 GO:0070646 GO:0070647 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090575 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1902493 GO:1902494 GO:1902680 GO:1903047 GO:1903506 GO:1903508 GO:1990234 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko03022 ko05168 map03022 map05168
Pfam Domains
Protein Families
TFIID_30kDa

Protein Analysis

134

Amino Acids

14.91

Weight (kDa)

4.79

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAF10 PF03540 25 - 118 4.5e-35 Transcription initiation factor TFIID 23-30kDa subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 48, 69, 153, 356
AclWI GGATC 2 cut(s) 374, 387
AcuI CTGAAG 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 66
AfiI CCNNNNNNNGG 2 cut(s) 44, 371
AluBI AGCT 2 cut(s) 127, 194
AluI AGCT 2 cut(s) 127, 194
Alw21I GWGCWC 1 cut(s) 129
AlwI GGATC 2 cut(s) 374, 387
AseI ATTAAT 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 67
BamHI GGATCC 1 cut(s) 379
BanII GRGCYC 1 cut(s) 129
BauI CACGAG 2 cut(s) 56, 128
Bbv12I GWGCWC 1 cut(s) 129
BccI CCATC 2 cut(s) 91, 370
BceAI ACGGC 1 cut(s) 61
BfmI CTRYAG 1 cut(s) 267
BglII AGATCT 1 cut(s) 298
BisI GCNGC 2 cut(s) 69, 154
BlsI GCNGC 2 cut(s) 70, 155
BmiI GGNNCC 1 cut(s) 381
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 66
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 371
Bse1I ACTGG 1 cut(s) 376
Bse3DI GCAATG 1 cut(s) 251
BseGI GGATG 2 cut(s) 67, 381
BseLI CCNNNNNNNGG 2 cut(s) 44, 371
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 1 cut(s) 376
BsiHKAI GWGCWC 1 cut(s) 129
BslI CCNNNNNNNGG 2 cut(s) 44, 371
Bsp1286I GDGCHC 1 cut(s) 129
Bsp143I GATC 2 cut(s) 298, 379
BspACI CCGC 4 cut(s) 48, 69, 153, 356
BspCNI CTCAG 1 cut(s) 211
BspLI GGNNCC 1 cut(s) 381
BspPI GGATC 2 cut(s) 374, 387
BspQI GCTCTTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 251
BsrI ACTGG 1 cut(s) 376
BssMI GATC 2 cut(s) 298, 379
BssNI GRCGYC 1 cut(s) 66
BssSI CACGAG 2 cut(s) 56, 128
Bst2BI CACGAG 2 cut(s) 56, 128
Bst6I CTCTTC 2 cut(s) 243, 390
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 219
BstF5I GGATG 2 cut(s) 67, 381
BstKTI GATC 2 cut(s) 301, 382
BstMBI GATC 2 cut(s) 298, 379
BstMWI GCNNNNNNNGC 5 cut(s) 124, 133, 162, 233, 260
BstSFI CTRYAG 1 cut(s) 267
BstX2I RGATCY 2 cut(s) 298, 379
BstYI RGATCY 2 cut(s) 298, 379
BtsCI GGATG 2 cut(s) 67, 381
Cac8I GCNNGC 1 cut(s) 261
CseI GACGC 2 cut(s) 74, 266
CviAII CATG 1 cut(s) 115
CviJI RGCY 6 cut(s) 127, 156, 187, 194, 263, 363
CviKI_1 RGCY 6 cut(s) 127, 156, 187, 194, 263, 363
DdeI CTNAG 1 cut(s) 219
DpnI GATC 2 cut(s) 300, 381
DpnII GATC 2 cut(s) 298, 379
Eam1104I CTCTTC 2 cut(s) 243, 390
EarI CTCTTC 2 cut(s) 243, 390
EciI GGCGGA 1 cut(s) 63
Ecl136II GAGCTC 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 224
EcoICRI GAGCTC 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 129
FaeI CATG 1 cut(s) 118
FaiI YATR 3 cut(s) 116, 269, 324
FatI CATG 1 cut(s) 114
Fnu4HI GCNGC 2 cut(s) 69, 154
FokI GGATG 2 cut(s) 74, 388
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 2 cut(s) 69, 154
GluI GCNGC 2 cut(s) 69, 154
HgaI GACGC 2 cut(s) 74, 266
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 1 cut(s) 118
HphI GGTGA 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 21, 169, 220
Hpy188III TCNNGA 1 cut(s) 306
Hpy99I CGWCG 3 cut(s) 47, 125, 173
HpyAV CCTTC 1 cut(s) 375
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 2 cut(s) 249, 386
HpyF10VI GCNNNNNNNGC 5 cut(s) 124, 133, 162, 233, 260
HpyF3I CTNAG 1 cut(s) 219
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 1 cut(s) 118
Kzo9I GATC 2 cut(s) 298, 379
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 2 cut(s) 133, 170
LpnPI CCDG 7 cut(s) 20, 38, 44, 173, 245, 357, 396
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 171
MalI GATC 2 cut(s) 300, 381
MboI GATC 2 cut(s) 298, 379
MboII GAAGA 2 cut(s) 230, 308
MflI RGATCY 2 cut(s) 298, 379
MhlI GDGCHC 1 cut(s) 129
MluCI AATT 1 cut(s) 180
MmeI TCCRAC 1 cut(s) 192
MnlI CCTC 6 cut(s) 52, 83, 95, 100, 214, 249
MseI TTAA 2 cut(s) 179, 330
MslI CAYNNNNRTG 1 cut(s) 60
MwoI GCNNNNNNNGC 5 cut(s) 124, 133, 162, 233, 260
NdeII GATC 2 cut(s) 298, 379
NlaIII CATG 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 381
PciSI GCTCTTC 1 cut(s) 243
PkrI GCNGC 2 cut(s) 70, 155
PshBI ATTAAT 1 cut(s) 179
Psp124BI GAGCTC 1 cut(s) 129
PspN4I GGNNCC 1 cut(s) 381
PsuI RGATCY 2 cut(s) 298, 379
RseI CAYNNNNRTG 1 cut(s) 60
SacI GAGCTC 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 179, 330
SatI GCNGC 2 cut(s) 69, 154
Sau3AI GATC 2 cut(s) 298, 379
SduI GDGCHC 1 cut(s) 129
SetI ASST 4 cut(s) 129, 174, 196, 225
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 1 cut(s) 180
SsiI CCGC 4 cut(s) 48, 69, 153, 356
SspI AATATT 2 cut(s) 285, 350
SstI GAGCTC 1 cut(s) 129
TaiI ACGT 1 cut(s) 174
TaqI TCGA 2 cut(s) 175, 368
TasI AATT 1 cut(s) 180
TauI GCSGC 2 cut(s) 71, 156
Tru1I TTAA 2 cut(s) 179, 330
Tru9I TTAA 2 cut(s) 179, 330
TspDTI ATGAA 1 cut(s) 17
VspI ATTAAT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.