Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0012387

Physical Location & Seq

Genomic Coordinates Forward (+)

4798333 .. 4799472

Gene Structure 1140 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1070300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGAAAGACCACTCTCCGCCAAATCACTACGGCCAAACCACCGCCGTCCCCACCTCTCCTTCCACCCCACCGAAGATGGACGCCAGGATATGGAGCAAGCTGCCGGAGGAGCTCCTCGACCTTGTCCTTTCTTTCCTTCCTCTCAAAAACTTCCTGATTCTCAGATCGACCTGCAAGCGCTTCAAGTCGCTGTTATTCTCTCCCTCCTTCGTCTTCAAGCACTCCTCCGCCTTCCTCCTCCTCTCCCACCCTCAGTGCTTCCGCCACTTCCCTCTCTACGACTCCGACACCACCACCTGGCGCAAGCTACCTCTCTCTCTCTCCGCTCCGCTACCCTGCGCTGCGGGCGCAGCAGCACAAGCCTCCCTCCTCTGTGCCTCCAATGGGTTGCTCTGTTTCTCTCTCCCCAATTCGTTTCTCGTCTTCAATATTCTGACCAAGTCCTCGAGAGTGATCAAATTTCCAGATTGCCCTTTTGGATTCGAGCTTTTCTCTTTGATATCCACCCCTGCTGGGTATAATCTTTTCATGGCCTCTTCTAGGTCGTCGTCCAAAAGCACTTTTGTCTACCATTCAAAGGCGCGATCGTGGCAAAAGTTTGACTTTACCGAGACGATTTTAAGTGACAATTGTCACCAGAAGGGAGTTTACTTCAAAGGGTGTTTGTATTTCGTGACGCCGGAACCGTTTTCGATTGTCTGCTTCGAATTCGAGAGCGGGAAGTGGGAAAGACCGATTCCGGAGCTTCCTACAGAGCTCGTGTTTGCTCGGCTTGTCAGCGGAGGAGGAGAGAAGAAGCTGTATCTGATTGGTGGGGTCGGCAGGAATGGGATTGCCAGGAGCTTGCAAGTGTGGGAATTGGAATTTGGCGGTGGGATGAAGTGGGTGGAGGTGGAAAGCTTGCCGGACAGGATGTGCAAGAAACTGATGTCGGTTTGCTTCCATAACTACGAGCATCTGTACTGCTTTTGGCATCAGGGTTTGATCTGCGTCTGCTGCTACAATTGGCCGGAGATTTTGTACTTCAAGATCTCGAGGAGGACGTGGCATTGGATCCCCAAATGCCCGTCTCTGCCGGATAAATCCAGCTGCGGATTCCGGTGGTTTTCATTTGTACCGAACTTCCATTCATCCGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0031146 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box

Protein Analysis

380

Amino Acids

43.17

Weight (kDa)

9.02

Isoelectric Point (pI)

49.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	31 - 72	1.3e-08	F-box domain
F-box-like	PF12937	31 - 70	6.1e-07	F-box-like
Beta-prop_KIB1-4	PF03478	112 - 305	3e-09	KIB1-4 beta-propeller

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012387)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G43190
fragaria_vesca	FvH4_2g33130
malus_domestica	MD08G1084200.v1.1 MD15G1070300.v1.1
prunus_persica	Prupe.1G423100_v2.0.a1
pyrus_communis	pycom08g06980
rosa_chinensis	RchiOBHm_Chr6g0309541
rosa_laevigata	RLG00000010555
rosa_multiflora	Rmu_co8522085.1_g000003 Rmu_sc0007283.1_g000011 Rmu_sc0024715.1_g000004 Rmu_sc0030536.1_g000005
rosa_roxburghii	Rroxscaffold_7G00159320
rosa_rugosa	Rorug06G0372700
rosa_samantha	Rh6AG486100 Rh6BG495500 Rh6CG500500 Rh6DG486400
rosa_wichuraiana	Rw6G042340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	179
AccB7I	CCANNNNNTGG	2 cut(s)	90, 297
AccBSI	CCGCTC	2 cut(s)	326, 717
AccI	GTMKAC	1 cut(s)	567
AccII	CGCG	1 cut(s)	583
AccIII	TCCGGA	1 cut(s)	739
AclWI	GGATC	2 cut(s)	1048, 1061
AcoI	YGGCCR	2 cut(s)	31, 1007
AcsI	RAATTY	3 cut(s)	458, 707, 863
AcyI	GRCGYC	2 cut(s)	81, 677
AfaI	GTAC	3 cut(s)	962, 1022, 1116
AfeI	AGCGCT	1 cut(s)	179
AfiI	CCNNNNNNNGG	6 cut(s)	90, 297, 384, 513, 540, 577
AgsI	TTSAA	6 cut(s)	184, 217, 427, 576, 655, 1027
AjiI	CACGTC	1 cut(s)	1044
AjnI	CCWGG	3 cut(s)	83, 296, 836
AjuI	GAANNNNNNNTTGG	2 cut(s)	849, 881
Alw21I	GWGCWC	2 cut(s)	114, 759
Alw26I	GTCTC	2 cut(s)	605, 1074
AlwI	GGATC	2 cut(s)	1048, 1061
Ama87I	CYCGRG	2 cut(s)	445, 1033
Aor13HI	TCCGGA	1 cut(s)	739
Aor51HI	AGCGCT	1 cut(s)	179
AoxI	GGCC	3 cut(s)	31, 531, 1007
ApeKI	GCWGC	6 cut(s)	100, 341, 350, 353, 996, 1089
ApoI	RAATTY	3 cut(s)	458, 707, 863
AspLEI	GCGC	5 cut(s)	180, 303, 341, 350, 583
AsuHPI	GGTGA	1 cut(s)	626
AsuII	TTCGAA	1 cut(s)	705
AvaI	CYCGRG	2 cut(s)	445, 1033
BamHI	GGATCC	1 cut(s)	1053
BanII	GRGCYC	2 cut(s)	114, 759
BarI	GAAGNNNNNNTAC	2 cut(s)	632, 664
BauI	CACGAG	1 cut(s)	758
BbsI	GAAGAC	2 cut(s)	205, 415
Bbv12I	GWGCWC	2 cut(s)	114, 759
BbvI	GCAGC	6 cut(s)	87, 328, 362, 365, 983, 1076
BccI	CCATC	1 cut(s)	70
BceAI	ACGGC	2 cut(s)	29, 46
BciT130I	CCWGG	3 cut(s)	85, 298, 838
BclI	TGATCA	1 cut(s)	453
BcoDI	GTCTC	2 cut(s)	605, 1074
BfaI	CTAG	1 cut(s)	540
BfmI	CTRYAG	1 cut(s)	750
BfoI	RGCGCY	1 cut(s)	181
BfuAI	ACCTGC	1 cut(s)	179
BglII	AGATCT	1 cut(s)	1029
BisI	GCNGC	6 cut(s)	101, 342, 351, 354, 997, 1090
BlsI	GCNGC	6 cut(s)	102, 343, 352, 355, 998, 1091
Bme1390I	CCNGG	3 cut(s)	85, 298, 838
BmeT110I	CYCGRG	2 cut(s)	445, 1033
BmgBI	CACGTC	1 cut(s)	1044
BmiI	GGNNCC	2 cut(s)	684, 1055
BmrFI	CCNGG	3 cut(s)	85, 298, 838
BmsI	GCATC	2 cut(s)	964, 982
BoxI	GACNNNNGTC	1 cut(s)	630
BpiI	GAAGAC	2 cut(s)	205, 415
BplI	GAGNNNNNCTC	2 cut(s)	476, 508
Bpu14I	TTCGAA	1 cut(s)	705
BsaHI	GRCGYC	2 cut(s)	81, 677
BsaWI	WCCGGW	2 cut(s)	739, 1098
BsaXI	ACNNNNNCTCC	2 cut(s)	1004, 1034
Bsc4I	CCNNNNNNNGG	6 cut(s)	90, 297, 384, 513, 540, 577
BseAI	TCCGGA	1 cut(s)	739
BseBI	CCWGG	3 cut(s)	85, 298, 838
BseGI	GGATG	3 cut(s)	882, 918, 1130
BseLI	CCNNNNNNNGG	6 cut(s)	90, 297, 384, 513, 540, 577
BseMII	CTCAG	2 cut(s)	175, 266
BseRI	GAGGAG	9 cut(s)	104, 122, 214, 227, 230, 359, 798, 801, 1051
BseXI	GCAGC	6 cut(s)	87, 328, 362, 365, 983, 1076
BseYI	CCCAGC	1 cut(s)	512
Bsh1236I	CGCG	1 cut(s)	583
Bsh1285I	CGRYCG	1 cut(s)	587
BshFI	GGCC	3 cut(s)	33, 533, 1009
BsiEI	CGRYCG	1 cut(s)	587
BsiHKAI	GWGCWC	2 cut(s)	114, 759
BsiHKCI	CYCGRG	2 cut(s)	445, 1033
BsiSI	CCGG	7 cut(s)	104, 680, 740, 905, 1010, 1076, 1099
BslFI	GGGAC	1 cut(s)	32
BslI	CCNNNNNNNGG	6 cut(s)	90, 297, 384, 513, 540, 577
BsmAI	GTCTC	2 cut(s)	605, 1074
BsmBI	CGTCTC	2 cut(s)	605, 1074
BsmFI	GGGAC	1 cut(s)	32
BsnI	GGCC	3 cut(s)	33, 533, 1009
BsoBI	CYCGRG	2 cut(s)	445, 1033
Bsp119I	TTCGAA	1 cut(s)	705
Bsp1286I	GDGCHC	2 cut(s)	114, 759
Bsp13I	TCCGGA	1 cut(s)	739
Bsp143I	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
BspANI	GGCC	3 cut(s)	33, 533, 1009
BspCNI	CTCAG	2 cut(s)	174, 265
BspEI	TCCGGA	1 cut(s)	739
BspFNI	CGCG	1 cut(s)	583
BspLI	GGNNCC	2 cut(s)	684, 1055
BspMI	ACCTGC	1 cut(s)	179
BspPI	GGATC	2 cut(s)	1048, 1061
BspT104I	TTCGAA	1 cut(s)	705
BsrBI	CCGCTC	2 cut(s)	326, 717
BssMI	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
BssNI	GRCGYC	2 cut(s)	81, 677
BssSI	CACGAG	1 cut(s)	758
Bst2BI	CACGAG	1 cut(s)	758
Bst2UI	CCWGG	3 cut(s)	85, 298, 838
Bst4CI	ACNGT	1 cut(s)	687
Bst6I	CTCTTC	1 cut(s)	541
BstACI	GRCGYC	2 cut(s)	81, 677
BstBI	TTCGAA	1 cut(s)	705
BstC8I	GCNNGC	6 cut(s)	98, 176, 305, 346, 845, 902
BstDEI	CTNAG	2 cut(s)	161, 252
BstENI	CCTNNNNNAGG	1 cut(s)	538
BstF5I	GGATG	3 cut(s)	882, 918, 1130
BstFNI	CGCG	1 cut(s)	583
BstH2I	RGCGCY	1 cut(s)	181
BstHHI	GCGC	5 cut(s)	180, 303, 341, 350, 583
BstKTI	GATC	6 cut(s)	167, 456, 587, 987, 1032, 1056
BstMAI	GTCTC	2 cut(s)	605, 1074
BstMBI	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
BstMCI	CGRYCG	1 cut(s)	587
BstMWI	GCNNNNNNNGC	7 cut(s)	109, 345, 347, 350, 359, 589, 996
BstNI	CCWGG	3 cut(s)	85, 298, 838
BstPAI	GACNNNNGTC	1 cut(s)	630
BstSCI	CCNGG	3 cut(s)	83, 296, 836
BstSFI	CTRYAG	1 cut(s)	750
BstUI	CGCG	1 cut(s)	583
BstV1I	GCAGC	6 cut(s)	87, 328, 362, 365, 983, 1076
BstV2I	GAAGAC	2 cut(s)	205, 415
BstX2I	RGATCY	2 cut(s)	1029, 1053
BstYI	RGATCY	2 cut(s)	1029, 1053
BsuRI	GGCC	3 cut(s)	33, 533, 1009
BtrI	CACGTC	1 cut(s)	1044
BtsCI	GGATG	3 cut(s)	882, 918, 1130
BtsIMutI	CAGTG	1 cut(s)	260
BveI	ACCTGC	1 cut(s)	179
Cac8I	GCNNGC	6 cut(s)	98, 176, 305, 346, 845, 902
CfoI	GCGC	5 cut(s)	180, 303, 341, 350, 583
CseI	GACGC	3 cut(s)	89, 685, 979
Csp6I	GTAC	3 cut(s)	961, 1021, 1115
CviAII	CATG	1 cut(s)	529
CviQI	GTAC	3 cut(s)	961, 1021, 1115
DdeI	CTNAG	2 cut(s)	161, 252
DpnI	GATC	6 cut(s)	166, 455, 586, 986, 1031, 1055
DpnII	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
EaeI	YGGCCR	2 cut(s)	31, 1007
Eam1104I	CTCTTC	1 cut(s)	541
EarI	CTCTTC	1 cut(s)	541
EciI	GGCGGA	4 cut(s)	6, 217, 251, 1123
Ecl136II	GAGCTC	2 cut(s)	112, 757
Eco24I	GRGCYC	2 cut(s)	114, 759
Eco32I	GATATC	1 cut(s)	501
Eco47III	AGCGCT	1 cut(s)	179
Eco53kI	GAGCTC	2 cut(s)	112, 757
Eco88I	CYCGRG	2 cut(s)	445, 1033
EcoICRI	GAGCTC	2 cut(s)	112, 757
EcoNI	CCTNNNNNAGG	1 cut(s)	538
EcoRI	GAATTC	1 cut(s)	707
EcoRII	CCWGG	3 cut(s)	83, 296, 836
EcoRV	GATATC	1 cut(s)	501
EcoT38I	GRGCYC	2 cut(s)	114, 759
Esp3I	CGTCTC	2 cut(s)	605, 1074
FaeI	CATG	1 cut(s)	532
FaiI	YATR	4 cut(s)	91, 519, 530, 945
FalI	AAGNNNNNCTT	2 cut(s)	587, 619
FaqI	GGGAC	1 cut(s)	32
FatI	CATG	1 cut(s)	528
FauI	CCCGC	2 cut(s)	337, 710
FbaI	TGATCA	1 cut(s)	453
FblI	GTMKAC	1 cut(s)	567
Fnu4HI	GCNGC	6 cut(s)	101, 342, 351, 354, 997, 1090
FokI	GGATG	3 cut(s)	889, 925, 1117
FriOI	GRGCYC	2 cut(s)	114, 759
Fsp4HI	GCNGC	6 cut(s)	101, 342, 351, 354, 997, 1090
FspBI	CTAG	1 cut(s)	540
GlaI	GCGC	5 cut(s)	179, 302, 340, 349, 582
GluI	GCNGC	6 cut(s)	101, 342, 351, 354, 997, 1090
GsaI	CCCAGC	1 cut(s)	516
HaeII	RGCGCY	1 cut(s)	181
HaeIII	GGCC	3 cut(s)	33, 533, 1009
HapII	CCGG	7 cut(s)	104, 680, 740, 905, 1010, 1076, 1099
HgaI	GACGC	3 cut(s)	89, 685, 979
HhaI	GCGC	5 cut(s)	180, 303, 341, 350, 583
Hin1I	GRCGYC	2 cut(s)	81, 677
Hin1II	CATG	1 cut(s)	532
Hin6I	GCGC	5 cut(s)	178, 301, 339, 348, 581
HinP1I	GCGC	5 cut(s)	178, 301, 339, 348, 581
HindIII	AAGCTT	1 cut(s)	898
HinfI	GANTC	5 cut(s)	157, 281, 480, 736, 1095
HpaII	CCGG	7 cut(s)	104, 680, 740, 905, 1010, 1076, 1099
HphI	GGTGA	1 cut(s)	626
Hpy166II	GTNNAC	2 cut(s)	568, 649
Hpy188I	TCNGA	4 cut(s)	164, 286, 435, 807
Hpy188III	TCNNGA	8 cut(s)	154, 447, 464, 673, 712, 740, 1027, 1033
Hpy8I	GTNNAC	2 cut(s)	568, 649
Hpy99I	CGWCG	1 cut(s)	550
HpyAV	CCTTC	5 cut(s)	69, 146, 217, 241, 634
HpyCH4III	ACNGT	1 cut(s)	687
HpyCH4IV	ACGT	1 cut(s)	1043
HpyCH4V	TGCA	3 cut(s)	174, 847, 918
HpyF10VI	GCNNNNNNNGC	7 cut(s)	109, 345, 347, 350, 359, 589, 996
HpyF3I	CTNAG	2 cut(s)	161, 252
HpySE526I	ACGT	1 cut(s)	1043
Hsp92I	GRCGYC	2 cut(s)	81, 677
Hsp92II	CATG	1 cut(s)	532
HspAI	GCGC	5 cut(s)	178, 301, 339, 348, 581
Kpn2I	TCCGGA	1 cut(s)	739
Ksp22I	TGATCA	1 cut(s)	453
Kzo9I	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
LmnI	GCTCC	6 cut(s)	93, 109, 117, 331, 742, 840
Lsp1109I	GCAGC	6 cut(s)	87, 328, 362, 365, 983, 1076
LweI	GCATC	2 cut(s)	964, 982
MaeI	CTAG	1 cut(s)	540
MaeII	ACGT	1 cut(s)	1043
MaeIII	GTNAC	3 cut(s)	623, 632, 673
MalI	GATC	6 cut(s)	166, 455, 586, 986, 1031, 1055
MbiI	CCGCTC	2 cut(s)	326, 717
MboI	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
MboII	GAAGA	5 cut(s)	85, 205, 415, 528, 805
MfeI	CAATTG	2 cut(s)	628, 1003
MflI	RGATCY	2 cut(s)	1029, 1053
MhlI	GDGCHC	2 cut(s)	114, 759
MluCI	AATT	7 cut(s)	409, 458, 628, 707, 857, 863, 1003
MlyI	GAGTC	1 cut(s)	275
MmeI	TCCRAC	1 cut(s)	309
MroI	TCCGGA	1 cut(s)	739
MseI	TTAA	1 cut(s)	620
MspA1I	CMGCKG	2 cut(s)	780, 1089
MspI	CCGG	7 cut(s)	104, 680, 740, 905, 1010, 1076, 1099
MspR9I	CCNGG	3 cut(s)	85, 298, 838
MunI	CAATTG	2 cut(s)	628, 1003
MvaI	CCWGG	3 cut(s)	85, 298, 838
MvnI	CGCG	1 cut(s)	583
MwoI	GCNNNNNNNGC	7 cut(s)	109, 345, 347, 350, 359, 589, 996
NdeII	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
NlaIII	CATG	1 cut(s)	532
NlaIV	GGNNCC	2 cut(s)	684, 1055
NmeAIII	GCCGAG	1 cut(s)	748
NmuCI	GTSAC	3 cut(s)	623, 632, 673
NspV	TTCGAA	1 cut(s)	705
PaeR7I	CTCGAG	2 cut(s)	445, 1033
PcsI	WCGNNNNNNNCGW	2 cut(s)	683, 1040
PfeI	GAWTC	4 cut(s)	157, 480, 736, 1095
PflFI	GACNNNGTC	2 cut(s)	122, 439
PflMI	CCANNNNNTGG	2 cut(s)	90, 297
PkrI	GCNGC	6 cut(s)	102, 343, 352, 355, 998, 1091
Ple19I	CGATCG	1 cut(s)	587
PleI	GAGTC	1 cut(s)	275
PpsI	GAGTC	1 cut(s)	275
PshAI	GACNNNNGTC	1 cut(s)	630
Psp124BI	GAGCTC	2 cut(s)	114, 759
Psp6I	CCWGG	3 cut(s)	83, 296, 836
PspFI	CCCAGC	1 cut(s)	512
PspGI	CCWGG	3 cut(s)	83, 296, 836
PspN4I	GGNNCC	2 cut(s)	684, 1055
PsuI	RGATCY	2 cut(s)	1029, 1053
PsyI	GACNNNGTC	2 cut(s)	122, 439
PvuI	CGATCG	1 cut(s)	587
PvuII	CAGCTG	1 cut(s)	1089
RsaI	GTAC	3 cut(s)	962, 1022, 1116
RsaNI	GTAC	3 cut(s)	961, 1021, 1115
SacI	GAGCTC	2 cut(s)	114, 759
SaqAI	TTAA	1 cut(s)	620
SatI	GCNGC	6 cut(s)	101, 342, 351, 354, 997, 1090
Sau3AI	GATC	6 cut(s)	164, 453, 584, 984, 1029, 1053
SchI	GAGTC	1 cut(s)	275
ScrFI	CCNGG	3 cut(s)	85, 298, 838
SduI	GDGCHC	2 cut(s)	114, 759
SfaNI	GCATC	2 cut(s)	964, 982
SfcI	CTRYAG	1 cut(s)	750
Sfr274I	CTCGAG	2 cut(s)	445, 1033
SfuI	TTCGAA	1 cut(s)	705
SlaI	CTCGAG	2 cut(s)	445, 1033
SmlI	CTYRAG	2 cut(s)	445, 1033
SmoI	CTYRAG	2 cut(s)	445, 1033
Sse9I	AATT	7 cut(s)	409, 458, 628, 707, 857, 863, 1003
SspI	AATATT	1 cut(s)	430
SspMI	CTAG	1 cut(s)	540
SstI	GAGCTC	2 cut(s)	114, 759
StyD4I	CCNGG	3 cut(s)	83, 296, 836
TaaI	ACNGT	1 cut(s)	687
TaiI	ACGT	1 cut(s)	1046
TaqI	TCGA	8 cut(s)	117, 167, 446, 483, 692, 705, 711, 1034
TaqII	GACCGA	1 cut(s)	748
TasI	AATT	7 cut(s)	409, 458, 628, 707, 857, 863, 1003
TatI	WGTACW	2 cut(s)	960, 1020
TfiI	GAWTC	4 cut(s)	157, 480, 736, 1095
Tru1I	TTAA	1 cut(s)	620
Tru9I	TTAA	1 cut(s)	620
TscAI	CASTG	1 cut(s)	260
TseFI	GTSAC	3 cut(s)	623, 632, 673
TseI	GCWGC	6 cut(s)	100, 341, 350, 353, 996, 1089
Tsp45I	GTSAC	3 cut(s)	623, 632, 673
TspDTI	ATGAA	5 cut(s)	17, 517, 893, 1098, 1119
TspRI	CASTG	1 cut(s)	260
Tth111I	GACNNNGTC	2 cut(s)	122, 439
Van91I	CCANNNNNTGG	2 cut(s)	90, 297
XagI	CCTNNNNNAGG	1 cut(s)	538
XapI	RAATTY	3 cut(s)	458, 707, 863
XhoI	CTCGAG	2 cut(s)	445, 1033
XmiI	GTMKAC	1 cut(s)	567
XspI	CTAG	1 cut(s)	540

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.