MD15G1075800.v1.1

F-box protein GID2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0015383
Physical Location & Seq
Forward (+)
5172735 .. 5173331
597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1075800.v1.1.491

Sequence Viewer

Length: 597 bp
ATGAAGAGAGCATCATCGGGGGACGATTCGATGGCGGCGGCGAGTTCGGCGGATGTCAAGATGAAGAGGATTAAGGTGGAGGAAGAGGAGGAGGAGATGGTGGTTGACTGCGTTGACCAGGATGAGCTGAAAGGGGCGGGGTTTTCGAATCTGGACGACAATCTGATCTTCGAGGTGTTCAAGCACGTGGACGCGCGGACGTTGGGGGTGGCGTCGTGCGTGAGTAAGCAGTGGCACAAGACGGCGGAGGACGAGCGGCTCTGGGAGCTGATATGTACCAGGCAATGGGCTAACATCGGCTGCGGCAACCAGCAGCTGAGATCTGTGGTCCTCGCGCTTGGCGGATTCCGGCGGCTCCACTCCCTATCCATCTGGCCGTTCAGTAAGCCCTCCTCCGCCTCTTCCAGCTCTGCTCCTTCTTCTTCCTCGTCGGGTGCGTCCTCCTCGGCTTCGCCTTGGGCGACGGCTCCGGTGAAGCCGATGCCGAATCCAAAGCCGCCAACCGCTCGTTGGGGAAAAGACGAGGTGAATCTGTCGCTCTCGCTTCTCTCGATTCGGTTTTATGAGAAGATGAACTACGCGAACAGGGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box

Protein Analysis

199

Amino Acids

21.73

Weight (kDa)

7.67

Isoelectric Point (pI)

62.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 48 - 89 9e-09 F-box domain
F-box-like PF12937 54 - 93 9.5e-08 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24210
fragaria_vesca FvH4_2g33690
malus_domestica MD08G1091700.v1.1 MD15G1075800.v1.1
prunus_persica Prupe.1G429300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0308861
rosa_laevigata RLG00000010612
rosa_multiflora Rmu_co8338391.1_g000002 Rmu_sc0004046.1_g000011
rosa_roxburghii Rroxscaffold_7G00159850
rosa_rugosa Rorug06G0368700
rosa_samantha Rh6AG480200 Rh6BG489700 Rh6CG495100 Rh6DG480500
rosa_wichuraiana Rw6G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 285
AccBSI CCGCTC 2 cut(s) 256, 506
AccII CGCG 4 cut(s) 194, 196, 335, 581
AcoI YGGCCR 1 cut(s) 374
AcvI CACGTG 1 cut(s) 187
AcyI GRCGYC 1 cut(s) 212
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 2 cut(s) 285, 510
AgsI TTSAA 1 cut(s) 181
AjnI CCWGG 2 cut(s) 117, 278
AluBI AGCT 4 cut(s) 127, 268, 316, 408
AluI AGCT 4 cut(s) 127, 268, 316, 408
AlwNI CAGNNNCTG 1 cut(s) 316
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 2 cut(s) 300, 313
AspLEI GCGC 2 cut(s) 196, 337
AspS9I GGNCC 1 cut(s) 328
AsuHPI GGTGA 2 cut(s) 484, 538
AsuII TTCGAA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 328
BarI GAAGNNNNNNTAC 2 cut(s) 560, 592
BbrPI CACGTG 1 cut(s) 187
BbvI GCAGC 2 cut(s) 287, 325
BccI CCATC 3 cut(s) 25, 91, 377
BceAI ACGGC 3 cut(s) 258, 361, 480
BciT130I CCWGG 2 cut(s) 119, 280
BglII AGATCT 1 cut(s) 320
BisI GCNGC 8 cut(s) 36, 39, 257, 301, 304, 314, 353, 497
BlsI GCNGC 8 cut(s) 37, 40, 258, 302, 305, 315, 354, 498
Bme1390I CCNGG 2 cut(s) 119, 280
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 1 cut(s) 328
BmiI GGNNCC 2 cut(s) 356, 468
BmrFI CCNGG 2 cut(s) 119, 280
BmsI GCATC 2 cut(s) 20, 471
Bpu14I TTCGAA 1 cut(s) 146
BsaAI YACGTR 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 212
BsaJI CCNNGG 2 cut(s) 444, 455
BsaWI WCCGGW 1 cut(s) 469
Bsc4I CCNNNNNNNGG 2 cut(s) 285, 510
Bse3DI GCAATG 1 cut(s) 290
BseBI CCWGG 2 cut(s) 119, 280
BseDI CCNNGG 2 cut(s) 444, 455
BseGI GGATG 2 cut(s) 58, 127
BseLI CCNNNNNNNGG 2 cut(s) 285, 510
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 1 cut(s) 308
BseRI GAGGAG 5 cut(s) 101, 104, 107, 382, 433
BseXI GCAGC 2 cut(s) 287, 325
Bsh1236I CGCG 4 cut(s) 194, 196, 335, 581
BshFI GGCC 1 cut(s) 376
BsiSI CCGG 2 cut(s) 349, 470
BslFI GGGAC 1 cut(s) 35
BslI CCNNNNNNNGG 2 cut(s) 285, 510
BsmFI GGGAC 1 cut(s) 35
BsnI GGCC 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 146
Bsp143I GATC 2 cut(s) 165, 320
BspANI GGCC 1 cut(s) 376
BspCNI CTCAG 1 cut(s) 309
BspFNI CGCG 4 cut(s) 194, 196, 335, 581
BspLI GGNNCC 2 cut(s) 356, 468
BspT104I TTCGAA 1 cut(s) 146
BsrBI CCGCTC 2 cut(s) 256, 506
BsrDI GCAATG 1 cut(s) 290
BssECI CCNNGG 2 cut(s) 444, 455
BssMI GATC 2 cut(s) 165, 320
BssNI GRCGYC 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 2 cut(s) 119, 280
Bst6I CTCTTC 3 cut(s) 59, 78, 406
BstACI GRCGYC 1 cut(s) 212
BstBAI YACGTR 1 cut(s) 187
BstBI TTCGAA 1 cut(s) 146
BstDEI CTNAG 1 cut(s) 317
BstF5I GGATG 2 cut(s) 58, 127
BstFNI CGCG 4 cut(s) 194, 196, 335, 581
BstHHI GCGC 2 cut(s) 196, 337
BstKTI GATC 2 cut(s) 168, 323
BstMBI GATC 2 cut(s) 165, 320
BstMWI GCNNNNNNNGC 2 cut(s) 47, 265
BstNI CCWGG 2 cut(s) 119, 280
BstSCI CCNGG 2 cut(s) 117, 278
BstUI CGCG 4 cut(s) 194, 196, 335, 581
BstV1I GCAGC 2 cut(s) 287, 325
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 1 cut(s) 376
BtsCI GGATG 2 cut(s) 58, 127
BtsI GCAGTG 1 cut(s) 236
BtsIMutI CAGTG 1 cut(s) 236
CaiI CAGNNNCTG 1 cut(s) 316
CfoI GCGC 2 cut(s) 196, 337
Cfr13I GGNCC 1 cut(s) 328
CseI GACGC 3 cut(s) 200, 201, 426
Csp6I GTAC 1 cut(s) 276
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 1 cut(s) 317
DpnI GATC 2 cut(s) 167, 322
DpnII GATC 2 cut(s) 165, 320
EaeI YGGCCR 1 cut(s) 374
Eam1104I CTCTTC 3 cut(s) 59, 78, 406
EarI CTCTTC 3 cut(s) 59, 78, 406
EciI GGCGGA 4 cut(s) 65, 260, 357, 385
Eco130I CCWWGG 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 328
Eco72I CACGTG 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 117, 278
EcoT14I CCWWGG 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 455
FaiI YATR 2 cut(s) 274, 564
FaqI GGGAC 1 cut(s) 35
FauI CCCGC 1 cut(s) 130
Fnu4HI GCNGC 8 cut(s) 36, 39, 257, 301, 304, 314, 353, 497
FokI GGATG 2 cut(s) 65, 134
Fsp4HI GCNGC 8 cut(s) 36, 39, 257, 301, 304, 314, 353, 497
GlaI GCGC 2 cut(s) 195, 336
GluI GCNGC 8 cut(s) 36, 39, 257, 301, 304, 314, 353, 497
HaeIII GGCC 1 cut(s) 376
HapII CCGG 2 cut(s) 349, 470
HgaI GACGC 3 cut(s) 200, 201, 426
HhaI GCGC 2 cut(s) 196, 337
Hin1I GRCGYC 1 cut(s) 212
Hin6I GCGC 2 cut(s) 194, 335
HinP1I GCGC 2 cut(s) 194, 335
HincII GTYRAC 2 cut(s) 106, 115
HindII GTYRAC 2 cut(s) 106, 115
HinfI GANTC 6 cut(s) 26, 148, 345, 487, 529, 553
HpaII CCGG 2 cut(s) 349, 470
HphI GGTGA 2 cut(s) 484, 538
Hpy166II GTNNAC 3 cut(s) 106, 115, 190
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 3 cut(s) 58, 152, 550
Hpy8I GTNNAC 3 cut(s) 106, 115, 190
Hpy99I CGWCG 3 cut(s) 217, 433, 466
HpyAV CCTTC 1 cut(s) 426
HpyCH4IV ACGT 2 cut(s) 186, 200
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 265
HpyF3I CTNAG 1 cut(s) 317
HpySE526I ACGT 2 cut(s) 186, 200
Hsp92I GRCGYC 1 cut(s) 212
HspAI GCGC 2 cut(s) 194, 335
Kzo9I GATC 2 cut(s) 165, 320
LmnI GCTCC 4 cut(s) 265, 360, 418, 472
Lsp1109I GCAGC 2 cut(s) 287, 325
LweI GCATC 2 cut(s) 20, 471
MaeII ACGT 2 cut(s) 186, 200
MalI GATC 2 cut(s) 167, 322
MbiI CCGCTC 2 cut(s) 256, 506
MboI GATC 2 cut(s) 165, 320
MboII GAAGA 8 cut(s) 16, 76, 95, 160, 393, 411, 414, 580
MflI RGATCY 1 cut(s) 320
MseI TTAA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 2 cut(s) 349, 470
MspR9I CCNGG 2 cut(s) 119, 280
MvaI CCWGG 2 cut(s) 119, 280
MvnI CGCG 4 cut(s) 194, 196, 335, 581
MwoI GCNNNNNNNGC 2 cut(s) 47, 265
NdeII GATC 2 cut(s) 165, 320
NlaIV GGNNCC 2 cut(s) 356, 468
NmeAIII GCCGAG 1 cut(s) 425
NspV TTCGAA 1 cut(s) 146
PcsI WCGNNNNNNNCGW 3 cut(s) 434, 458, 548
PfeI GAWTC 6 cut(s) 26, 148, 345, 487, 529, 553
PflMI CCANNNNNTGG 1 cut(s) 285
PkrI GCNGC 8 cut(s) 37, 40, 258, 302, 305, 315, 354, 498
PmaCI CACGTG 1 cut(s) 187
PmlI CACGTG 1 cut(s) 187
Ppu21I YACGTR 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 117, 278
PspCI CACGTG 1 cut(s) 187
PspGI CCWGG 2 cut(s) 117, 278
PspN4I GGNNCC 2 cut(s) 356, 468
PspPI GGNCC 1 cut(s) 328
PstNI CAGNNNCTG 1 cut(s) 316
PsuI RGATCY 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 316
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
SaqAI TTAA 1 cut(s) 72
SatI GCNGC 8 cut(s) 36, 39, 257, 301, 304, 314, 353, 497
Sau3AI GATC 2 cut(s) 165, 320
Sau96I GGNCC 1 cut(s) 328
ScrFI CCNGG 2 cut(s) 119, 280
SetI ASST 9 cut(s) 78, 129, 177, 189, 203, 270, 318, 410, 528
SfaNI GCATC 2 cut(s) 20, 471
SfuI TTCGAA 1 cut(s) 146
SinI GGWCC 1 cut(s) 328
StyD4I CCNGG 2 cut(s) 117, 278
StyI CCWWGG 1 cut(s) 455
TaiI ACGT 2 cut(s) 189, 203
TaqI TCGA 4 cut(s) 29, 146, 171, 551
TauI GCSGC 6 cut(s) 38, 41, 259, 306, 355, 499
TfiI GAWTC 6 cut(s) 26, 148, 345, 487, 529, 553
Tru1I TTAA 1 cut(s) 72
Tru9I TTAA 1 cut(s) 72
TscAI CASTG 1 cut(s) 236
TseI GCWGC 2 cut(s) 300, 313
TspDTI ATGAA 3 cut(s) 17, 77, 587
TspRI CASTG 1 cut(s) 236
Van91I CCANNNNNTGG 1 cut(s) 285
VpaK11BI GGWCC 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.