Rw6G041860

F-box protein GID2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015383
Physical Location & Seq
Reverse (-)
65487843 .. 65489232
1390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G041860.1

Sequence Viewer

Length: 573 bp
ATGAAGCGTGGGTTTTCCATGGCGGAGGAGGGCGATAATAAGATGAAGAAGTTAAAGGTGGAAGAAGAAGGGGAAGACAAGATTGAGCAGCAGCAGCAAGAAGAGGAGGTTGTCATCGTTCAAGGCCCGGGCTTTACGAATCTGGACGATAATCTGATATTCGAGGTGTTTAAGCACGTTGATGCCAGAACACTGGGAATGGCGTCGTGTGTGAGCAAGCAGTGGCACAAGACCGCCCAGGACGAGCGGCTCTGGGAGCTCATCTGCACCCGCCAGTGGGCTAACATCGGCTGCGGTAATCAGCAGCTGAGATCTGTCGTCCTCGCCCTCGGTGGCTTCCGGAGGCTCCACTCCCTCTACATCTGGCCTCTCACTAAGTCTTCTCCGTGCTCTTTATCGTCCTTGTCTTCTTCTTCTTCTTCGTCGTCGTCGTGGCCGGCTCCGCTGAAGCCGATGGTGGCCGCCAAGCCGCACGCTAGGTGGGGGAAGGATGAGGTGAATCTTTCGCTCTCGCTTCTGTCGATTCGGTTTTATGAGAAGATGAATTACAGCAACAGAGGCCCAGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box

Protein Analysis

190

Amino Acids

21.48

Weight (kDa)

8.67

Isoelectric Point (pI)

62.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 46 - 86 6.6e-08 F-box domain
F-box-like PF12937 52 - 90 1.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24210
fragaria_vesca FvH4_2g33690
malus_domestica MD08G1091700.v1.1 MD15G1075800.v1.1
prunus_persica Prupe.1G429300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0308861
rosa_laevigata RLG00000010612
rosa_multiflora Rmu_co8338391.1_g000002 Rmu_sc0004046.1_g000011
rosa_roxburghii Rroxscaffold_7G00159850
rosa_rugosa Rorug06G0368700
rosa_samantha Rh6AG480200 Rh6BG489700 Rh6CG495100 Rh6DG480500
rosa_wichuraiana Rw6G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 339
AciI CCGC 8 cut(s) 23, 234, 247, 271, 294, 443, 462, 470
AcoI YGGCCR 2 cut(s) 434, 459
AcuI CTGAAG 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 276, 277
AgsI TTSAA 1 cut(s) 122
AjnI CCWGG 2 cut(s) 237, 562
AluBI AGCT 2 cut(s) 259, 307
AluI AGCT 2 cut(s) 259, 307
Alw21I GWGCWC 2 cut(s) 261, 392
AlwNI CAGNNNCTG 1 cut(s) 307
Ama87I CYCGRG 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 339
AoxI GGCC 5 cut(s) 124, 365, 434, 459, 559
ApeKI GCWGC 5 cut(s) 88, 91, 94, 291, 304
AspS9I GGNCC 2 cut(s) 125, 560
AsuC2I CCSGG 2 cut(s) 128, 129
AsuHPI GGTGA 1 cut(s) 508
AvaI CYCGRG 1 cut(s) 127
BanII GRGCYC 1 cut(s) 261
BarI GAAGNNNNNNTAC 2 cut(s) 530, 562
BbsI GAAGAC 3 cut(s) 81, 372, 399
Bbv12I GWGCWC 2 cut(s) 261, 392
BbvI GCAGC 5 cut(s) 100, 103, 106, 278, 316
BccI CCATC 1 cut(s) 448
BciT130I CCWGG 2 cut(s) 239, 564
BcnI CCSGG 2 cut(s) 128, 129
BfaI CTAG 1 cut(s) 477
BglII AGATCT 1 cut(s) 311
BisI GCNGC 8 cut(s) 89, 92, 95, 248, 292, 305, 462, 470
BlsI GCNGC 8 cut(s) 90, 93, 96, 249, 293, 306, 463, 471
Bme1390I CCNGG 4 cut(s) 128, 129, 239, 564
BmeT110I CYCGRG 1 cut(s) 127
BmgT120I GGNCC 2 cut(s) 125, 560
BmiI GGNNCC 2 cut(s) 347, 441
BmrFI CCNGG 4 cut(s) 128, 129, 239, 564
BmrI ACTGGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 203
BpiI GAAGAC 3 cut(s) 81, 372, 399
BpuMI CCSGG 2 cut(s) 128, 129
BsaHI GRCGYC 1 cut(s) 203
BsaJI CCNNGG 5 cut(s) 18, 127, 237, 328, 562
BsaWI WCCGGW 1 cut(s) 339
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 277
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 2 cut(s) 198, 274
BseAI TCCGGA 1 cut(s) 339
BseBI CCWGG 2 cut(s) 239, 564
BseDI CCNNGG 5 cut(s) 18, 127, 237, 328, 562
BseGI GGATG 1 cut(s) 496
BseLI CCNNNNNNNGG 2 cut(s) 276, 277
BseMII CTCAG 1 cut(s) 299
BseNI ACTGG 2 cut(s) 198, 274
BseRI GAGGAG 2 cut(s) 41, 119
BseXI GCAGC 5 cut(s) 100, 103, 106, 278, 316
BsgI GTGCAG 1 cut(s) 250
BshFI GGCC 5 cut(s) 126, 367, 436, 461, 561
BsiHKAI GWGCWC 2 cut(s) 261, 392
BsiHKCI CYCGRG 1 cut(s) 127
BsiSI CCGG 3 cut(s) 128, 340, 437
BslI CCNNNNNNNGG 2 cut(s) 276, 277
BsnI GGCC 5 cut(s) 126, 367, 436, 461, 561
BsoBI CYCGRG 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 261, 392
Bsp13I TCCGGA 1 cut(s) 339
Bsp143I GATC 1 cut(s) 311
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 8 cut(s) 23, 234, 247, 271, 294, 443, 462, 470
BspANI GGCC 5 cut(s) 126, 367, 436, 461, 561
BspCNI CTCAG 1 cut(s) 300
BspEI TCCGGA 1 cut(s) 339
BspLI GGNNCC 2 cut(s) 347, 441
BsrBI CCGCTC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 2 cut(s) 198, 274
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 5 cut(s) 18, 127, 237, 328, 562
BssMI GATC 1 cut(s) 311
BssNI GRCGYC 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 239, 564
Bst6I CTCTTC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 203
BstC8I GCNNGC 3 cut(s) 218, 438, 474
BstDEI CTNAG 2 cut(s) 308, 375
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 496
BstKTI GATC 1 cut(s) 314
BstMBI GATC 1 cut(s) 311
BstMWI GCNNNNNNNGC 4 cut(s) 94, 256, 442, 558
BstNI CCWGG 2 cut(s) 239, 564
BstSCI CCNGG 4 cut(s) 126, 127, 237, 562
BstV1I GCAGC 5 cut(s) 100, 103, 106, 278, 316
BstV2I GAAGAC 3 cut(s) 81, 372, 399
BstX2I RGATCY 1 cut(s) 311
BstXI CCANNNNNNTGG 1 cut(s) 193
BstYI RGATCY 1 cut(s) 311
BsuRI GGCC 5 cut(s) 126, 367, 436, 461, 561
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 227
BtsIMutI CAGTG 3 cut(s) 191, 227, 281
Cac8I GCNNGC 3 cut(s) 218, 438, 474
CaiI CAGNNNCTG 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 2 cut(s) 125, 560
Cfr9I CCCGGG 1 cut(s) 127
CseI GACGC 1 cut(s) 192
CviAII CATG 1 cut(s) 19
DdeI CTNAG 2 cut(s) 308, 375
DpnI GATC 1 cut(s) 313
DpnII GATC 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 434, 459
Eam1104I CTCTTC 1 cut(s) 96
EarI CTCTTC 1 cut(s) 96
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 261
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 467
Eco88I CYCGRG 1 cut(s) 127
EcoICRI GAGCTC 1 cut(s) 259
EcoRII CCWGG 2 cut(s) 237, 562
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 1 cut(s) 22
FaiI YATR 2 cut(s) 20, 534
FatI CATG 1 cut(s) 18
FauI CCCGC 1 cut(s) 278
Fnu4HI GCNGC 8 cut(s) 89, 92, 95, 248, 292, 305, 462, 470
FokI GGATG 1 cut(s) 503
FriOI GRGCYC 1 cut(s) 261
Fsp4HI GCNGC 8 cut(s) 89, 92, 95, 248, 292, 305, 462, 470
FspBI CTAG 1 cut(s) 477
GluI GCNGC 8 cut(s) 89, 92, 95, 248, 292, 305, 462, 470
HaeIII GGCC 5 cut(s) 126, 367, 436, 461, 561
HapII CCGG 3 cut(s) 128, 340, 437
HgaI GACGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 203
Hin1II CATG 1 cut(s) 22
HinfI GANTC 3 cut(s) 139, 499, 523
HpaII CCGG 3 cut(s) 128, 340, 437
HphI GGTGA 1 cut(s) 508
Hpy188I TCNGA 1 cut(s) 156
Hpy188III TCNNGA 2 cut(s) 143, 340
Hpy99I CGWCG 4 cut(s) 208, 427, 430, 433
HpyAV CCTTC 2 cut(s) 62, 481
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 267
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 256, 442, 558
HpyF3I CTNAG 2 cut(s) 308, 375
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 203
Hsp92II CATG 1 cut(s) 22
Kpn2I TCCGGA 1 cut(s) 339
KroI GCCGGC 1 cut(s) 436
KroNI GCCGGC 1 cut(s) 438
Kzo9I GATC 1 cut(s) 311
LmnI GCTCC 3 cut(s) 256, 351, 445
Lsp1109I GCAGC 5 cut(s) 100, 103, 106, 278, 316
LweI GCATC 1 cut(s) 172
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 177
MalI GATC 1 cut(s) 313
MbiI CCGCTC 1 cut(s) 247
MboI GATC 1 cut(s) 311
MflI RGATCY 1 cut(s) 311
MhlI GDGCHC 2 cut(s) 261, 392
MluCI AATT 1 cut(s) 544
MroI TCCGGA 1 cut(s) 339
MroNI GCCGGC 1 cut(s) 436
MseI TTAA 2 cut(s) 53, 171
MslI CAYNNNNRTG 1 cut(s) 180
MspA1I CMGCKG 2 cut(s) 307, 445
MspI CCGG 3 cut(s) 128, 340, 437
MspR9I CCNGG 4 cut(s) 128, 129, 239, 564
MvaI CCWGG 2 cut(s) 239, 564
MwoI GCNNNNNNNGC 4 cut(s) 94, 256, 442, 558
NaeI GCCGGC 1 cut(s) 438
NciI CCSGG 2 cut(s) 128, 129
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 311
NgoMIV GCCGGC 1 cut(s) 436
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 2 cut(s) 347, 441
PcsI WCGNNNNNNNCGW 2 cut(s) 428, 518
PdiI GCCGGC 1 cut(s) 438
PfeI GAWTC 3 cut(s) 139, 499, 523
PkrI GCNGC 8 cut(s) 90, 93, 96, 249, 293, 306, 463, 471
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 237, 562
PspGI CCWGG 2 cut(s) 237, 562
PspN4I GGNNCC 2 cut(s) 347, 441
PspPI GGNCC 2 cut(s) 125, 560
PstNI CAGNNNCTG 1 cut(s) 307
PsuI RGATCY 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 307
RseI CAYNNNNRTG 1 cut(s) 180
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 53, 171
SatI GCNGC 8 cut(s) 89, 92, 95, 248, 292, 305, 462, 470
Sau3AI GATC 1 cut(s) 311
Sau96I GGNCC 2 cut(s) 125, 560
ScrFI CCNGG 4 cut(s) 128, 129, 239, 564
SduI GDGCHC 2 cut(s) 261, 392
SetI ASST 8 cut(s) 60, 111, 168, 180, 261, 309, 482, 498
SfaNI GCATC 1 cut(s) 172
SmaI CCCGGG 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 180
SrfI GCCCGGGC 1 cut(s) 129
Sse9I AATT 1 cut(s) 544
SsiI CCGC 8 cut(s) 23, 234, 247, 271, 294, 443, 462, 470
SspMI CTAG 1 cut(s) 477
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 4 cut(s) 126, 127, 237, 562
StyI CCWWGG 1 cut(s) 18
TaiI ACGT 1 cut(s) 180
TaqI TCGA 2 cut(s) 162, 521
TasI AATT 1 cut(s) 544
TauI GCSGC 3 cut(s) 250, 464, 472
TfiI GAWTC 3 cut(s) 139, 499, 523
Tru1I TTAA 2 cut(s) 53, 171
Tru9I TTAA 2 cut(s) 53, 171
TscAI CASTG 3 cut(s) 198, 227, 281
TseI GCWGC 5 cut(s) 88, 91, 94, 291, 304
TspDTI ATGAA 3 cut(s) 17, 59, 557
TspGWI ACGGA 1 cut(s) 375
TspMI CCCGGG 1 cut(s) 127
TspRI CASTG 3 cut(s) 198, 227, 281
XmaI CCCGGG 1 cut(s) 127
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.