Rroxscaffold_7G00159850

F-box protein GID2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0015383
Physical Location & Seq
Forward (+)
2912402 .. 2912989
588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00159850.1

Sequence Viewer

Length: 588 bp
ATGAAGCGTGGGTTTTCCATGGCGGAGGAGGGCGATAATAAGATGAAGAAGTTAAAGGTGGAAGAAGAAGAAGGGGGAGACAAGATTGAGCAGCAGCAGCAAGAAGAGGAGGTTGTCATCTTCCAAGGCCCGGGCTTTACGAATCTGGACGATAATCTGATATTCGAGGTGTTTAAGCACGTTGATGCCAGAACACTGGGAATGGCGTCGTGTGTGAGCAAGCAGTGGCACAAGACCGCCCAGGACGAGCGTCTCTGGGAGCTCATCTGCACCCGTCAGTGGGCTAACATCGGCTGCGGTAATCAGCAGCTGAGATCTGTCGTCCTCGCCCTCGGTGGCTTCCGGAGGCTCCACTCCCTCTTCATCTGGCCTCTCACTAAGCCTTCTCCGTCCTCTTCGTCGTCCTTGTCTTCTTCTTCTTGTTCTTCTTCTTCGGCTCCGTCGTCGTGGGCGGCTCCGCTGAAGCCGATGATGGCCGCGAAGCCGCACGCTAGGTGGGGGAAGGATGAGGTGAATCTTTCGCTCTCGCTTCTGTCGATTCGGTTTTATGAGAAGATGAATTACAGCAACAGAGGCCCAGGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
F-box

Protein Analysis

195

Amino Acids

21.9

Weight (kDa)

8.68

Isoelectric Point (pI)

68.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 47 - 87 6.9e-08 F-box domain
F-box-like PF12937 53 - 91 1.1e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015383)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24210
fragaria_vesca FvH4_2g33690
malus_domestica MD08G1091700.v1.1 MD15G1075800.v1.1
prunus_persica Prupe.1G429300_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0308861
rosa_laevigata RLG00000010612
rosa_multiflora Rmu_co8338391.1_g000002 Rmu_sc0004046.1_g000011
rosa_roxburghii Rroxscaffold_7G00159850
rosa_rugosa Rorug06G0368700
rosa_samantha Rh6AG480200 Rh6BG489700 Rh6CG495100 Rh6DG480500
rosa_wichuraiana Rw6G041860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 479
AccIII TCCGGA 1 cut(s) 342
AciI CCGC 7 cut(s) 23, 237, 297, 452, 458, 477, 485
AcoI YGGCCR 1 cut(s) 474
AcuI CTGAAG 1 cut(s) 482
AcyI GRCGYC 1 cut(s) 206
AfiI CCNNNNNNNGG 3 cut(s) 130, 279, 280
AjnI CCWGG 2 cut(s) 240, 577
AluBI AGCT 2 cut(s) 262, 310
AluI AGCT 2 cut(s) 262, 310
Alw21I GWGCWC 1 cut(s) 264
Alw26I GTCTC 2 cut(s) 72, 257
AlwNI CAGNNNCTG 1 cut(s) 310
Ama87I CYCGRG 1 cut(s) 130
Aor13HI TCCGGA 1 cut(s) 342
AoxI GGCC 4 cut(s) 127, 368, 474, 574
ApeKI GCWGC 5 cut(s) 91, 94, 97, 294, 307
AspS9I GGNCC 2 cut(s) 128, 575
AsuC2I CCSGG 2 cut(s) 131, 132
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 130
BanII GRGCYC 1 cut(s) 264
BarI GAAGNNNNNNTAC 2 cut(s) 545, 577
BbsI GAAGAC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 264
BbvI GCAGC 5 cut(s) 103, 106, 109, 281, 319
BccI CCATC 1 cut(s) 466
BciT130I CCWGG 2 cut(s) 242, 579
BcnI CCSGG 2 cut(s) 131, 132
BcoDI GTCTC 2 cut(s) 72, 257
BfaI CTAG 1 cut(s) 492
BglII AGATCT 1 cut(s) 314
BisI GCNGC 8 cut(s) 92, 95, 98, 295, 308, 453, 477, 485
BlsI GCNGC 8 cut(s) 93, 96, 99, 296, 309, 454, 478, 486
Bme1390I CCNGG 4 cut(s) 131, 132, 242, 579
BmeT110I CYCGRG 1 cut(s) 130
BmgT120I GGNCC 2 cut(s) 128, 575
BmiI GGNNCC 3 cut(s) 350, 438, 456
BmrFI CCNGG 4 cut(s) 131, 132, 242, 579
BmrI ACTGGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 175
BmuI ACTGGG 1 cut(s) 206
BoxI GACNNNNGTC 1 cut(s) 249
BpiI GAAGAC 1 cut(s) 402
BpuMI CCSGG 2 cut(s) 131, 132
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 6 cut(s) 18, 124, 130, 240, 331, 577
BsaWI WCCGGW 1 cut(s) 342
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 279, 280
Bse1I ACTGG 1 cut(s) 201
BseAI TCCGGA 1 cut(s) 342
BseBI CCWGG 2 cut(s) 242, 579
BseDI CCNNGG 6 cut(s) 18, 124, 130, 240, 331, 577
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 3 cut(s) 130, 279, 280
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 201
BseRI GAGGAG 2 cut(s) 41, 122
BseXI GCAGC 5 cut(s) 103, 106, 109, 281, 319
BsgI GTGCAG 1 cut(s) 253
Bsh1236I CGCG 1 cut(s) 479
BshFI GGCC 4 cut(s) 129, 370, 476, 576
BsiHKAI GWGCWC 1 cut(s) 264
BsiHKCI CYCGRG 1 cut(s) 130
BsiSI CCGG 2 cut(s) 131, 343
BslI CCNNNNNNNGG 3 cut(s) 130, 279, 280
BsmAI GTCTC 2 cut(s) 72, 257
BsmBI CGTCTC 1 cut(s) 257
BsnI GGCC 4 cut(s) 129, 370, 476, 576
BsoBI CYCGRG 1 cut(s) 130
Bsp1286I GDGCHC 1 cut(s) 264
Bsp13I TCCGGA 1 cut(s) 342
Bsp143I GATC 1 cut(s) 314
Bsp19I CCATGG 1 cut(s) 18
BspACI CCGC 7 cut(s) 23, 237, 297, 452, 458, 477, 485
BspANI GGCC 4 cut(s) 129, 370, 476, 576
BspCNI CTCAG 1 cut(s) 303
BspEI TCCGGA 1 cut(s) 342
BspFNI CGCG 1 cut(s) 479
BspLI GGNNCC 3 cut(s) 350, 438, 456
BsrI ACTGG 1 cut(s) 201
BssECI CCNNGG 6 cut(s) 18, 124, 130, 240, 331, 577
BssMI GATC 1 cut(s) 314
BssNI GRCGYC 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 18, 124
Bst2UI CCWGG 2 cut(s) 242, 579
Bst6I CTCTTC 3 cut(s) 99, 365, 400
BstACI GRCGYC 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 221, 489
BstDEI CTNAG 2 cut(s) 311, 378
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 1 cut(s) 511
BstFNI CGCG 1 cut(s) 479
BstKTI GATC 1 cut(s) 317
BstMAI GTCTC 2 cut(s) 72, 257
BstMBI GATC 1 cut(s) 314
BstMWI GCNNNNNNNGC 2 cut(s) 97, 573
BstNI CCWGG 2 cut(s) 242, 579
BstPAI GACNNNNGTC 1 cut(s) 249
BstSCI CCNGG 4 cut(s) 129, 130, 240, 577
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 5 cut(s) 103, 106, 109, 281, 319
BstV2I GAAGAC 1 cut(s) 402
BstX2I RGATCY 1 cut(s) 314
BstXI CCANNNNNNTGG 1 cut(s) 196
BstYI RGATCY 1 cut(s) 314
BsuRI GGCC 4 cut(s) 129, 370, 476, 576
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 1 cut(s) 511
BtsI GCAGTG 1 cut(s) 230
BtsIMutI CAGTG 3 cut(s) 194, 230, 284
Cac8I GCNNGC 2 cut(s) 221, 489
CaiI CAGNNNCTG 1 cut(s) 310
Cfr13I GGNCC 2 cut(s) 128, 575
Cfr9I CCCGGG 1 cut(s) 130
CseI GACGC 2 cut(s) 195, 239
CviAII CATG 1 cut(s) 19
DdeI CTNAG 2 cut(s) 311, 378
DpnI GATC 1 cut(s) 316
DpnII GATC 1 cut(s) 314
EaeI YGGCCR 1 cut(s) 474
Eam1104I CTCTTC 3 cut(s) 99, 365, 400
EarI CTCTTC 3 cut(s) 99, 365, 400
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 262
Eco130I CCWWGG 2 cut(s) 18, 124
Eco24I GRGCYC 1 cut(s) 264
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 262
EcoRII CCWGG 2 cut(s) 240, 577
EcoT14I CCWWGG 2 cut(s) 18, 124
EcoT38I GRGCYC 1 cut(s) 264
ErhI CCWWGG 2 cut(s) 18, 124
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 1 cut(s) 22
FaiI YATR 2 cut(s) 20, 549
FatI CATG 1 cut(s) 18
Fnu4HI GCNGC 8 cut(s) 92, 95, 98, 295, 308, 453, 477, 485
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 8 cut(s) 92, 95, 98, 295, 308, 453, 477, 485
FspBI CTAG 1 cut(s) 492
GluI GCNGC 8 cut(s) 92, 95, 98, 295, 308, 453, 477, 485
HaeIII GGCC 4 cut(s) 129, 370, 476, 576
HapII CCGG 2 cut(s) 131, 343
HgaI GACGC 2 cut(s) 195, 239
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 1 cut(s) 22
HinfI GANTC 3 cut(s) 142, 514, 538
HpaII CCGG 2 cut(s) 131, 343
HphI GGTGA 1 cut(s) 523
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 2 cut(s) 146, 343
Hpy99I CGWCG 4 cut(s) 211, 403, 445, 448
HpyAV CCTTC 3 cut(s) 65, 393, 496
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 573
HpyF3I CTNAG 2 cut(s) 311, 378
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 1 cut(s) 22
Kpn2I TCCGGA 1 cut(s) 342
Kzo9I GATC 1 cut(s) 314
LmnI GCTCC 4 cut(s) 259, 354, 442, 460
Lsp1109I GCAGC 5 cut(s) 103, 106, 109, 281, 319
LweI GCATC 1 cut(s) 175
MaeI CTAG 1 cut(s) 492
MaeII ACGT 1 cut(s) 180
MalI GATC 1 cut(s) 316
MboI GATC 1 cut(s) 314
MflI RGATCY 1 cut(s) 314
MhlI GDGCHC 1 cut(s) 264
MluCI AATT 1 cut(s) 559
MroI TCCGGA 1 cut(s) 342
MseI TTAA 2 cut(s) 53, 174
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 2 cut(s) 310, 460
MspI CCGG 2 cut(s) 131, 343
MspR9I CCNGG 4 cut(s) 131, 132, 242, 579
MvaI CCWGG 2 cut(s) 242, 579
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 97, 573
NciI CCSGG 2 cut(s) 131, 132
NcoI CCATGG 1 cut(s) 18
NdeII GATC 1 cut(s) 314
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 3 cut(s) 350, 438, 456
PcsI WCGNNNNNNNCGW 2 cut(s) 440, 533
PfeI GAWTC 3 cut(s) 142, 514, 538
PkrI GCNGC 8 cut(s) 93, 96, 99, 296, 309, 454, 478, 486
PshAI GACNNNNGTC 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 264
Psp6I CCWGG 2 cut(s) 240, 577
PspGI CCWGG 2 cut(s) 240, 577
PspN4I GGNNCC 3 cut(s) 350, 438, 456
PspPI GGNCC 2 cut(s) 128, 575
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 310
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 2 cut(s) 53, 174
SatI GCNGC 8 cut(s) 92, 95, 98, 295, 308, 453, 477, 485
Sau3AI GATC 1 cut(s) 314
Sau96I GGNCC 2 cut(s) 128, 575
ScrFI CCNGG 4 cut(s) 131, 132, 242, 579
SduI GDGCHC 1 cut(s) 264
SetI ASST 8 cut(s) 60, 114, 171, 183, 264, 312, 497, 513
SfaNI GCATC 1 cut(s) 175
SmaI CCCGGG 1 cut(s) 132
SmiMI CAYNNNNRTG 1 cut(s) 183
SrfI GCCCGGGC 1 cut(s) 132
Sse9I AATT 1 cut(s) 559
SsiI CCGC 7 cut(s) 23, 237, 297, 452, 458, 477, 485
SspMI CTAG 1 cut(s) 492
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 4 cut(s) 129, 130, 240, 577
StyI CCWWGG 2 cut(s) 18, 124
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 165, 536
TasI AATT 1 cut(s) 559
TauI GCSGC 3 cut(s) 455, 479, 487
TfiI GAWTC 3 cut(s) 142, 514, 538
Tru1I TTAA 2 cut(s) 53, 174
Tru9I TTAA 2 cut(s) 53, 174
TscAI CASTG 3 cut(s) 201, 230, 284
TseI GCWGC 5 cut(s) 91, 94, 97, 294, 307
TspDTI ATGAA 4 cut(s) 17, 59, 352, 572
TspGWI ACGGA 2 cut(s) 378, 429
TspMI CCCGGG 1 cut(s) 130
TspRI CASTG 3 cut(s) 201, 230, 284
XmaI CCCGGG 1 cut(s) 130
XspI CTAG 1 cut(s) 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.