Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0006520

Physical Location & Seq

Genomic Coordinates Forward (+)

5179022 .. 5182373

Gene Structure 3352 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1076200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 999 bp

ATGAGACGCAAAGGCTCCAAGTCCAAGACTGCGGCGAGTGATACCACTGACACTGACTCGTCCGCCGCCGAGTCCGACAGCTCCGCCACCAAGACCGATTCCGACAAGATCCACGACGAAAACTACGACGCTTCGCCCCCTTCCCCCGATTCCTGCCCCTTTACCGAGGGCGAGAAGGCCCTCGCCTTTCACAAGGCACGACTGTACGAGGCCAAGGTAACCGATGTCCGATATAAGATGGATTGGGAGTTTTATGTTCATTACGCTGGATGGAACAAAAGTTGGGATGAGTGGGTAGGGTTGGATCGTCTGATGAAAAACACTGACGAGAATAAGAAGAAACACCAGTACCTCAACCAGAAACAAGGAACAGACAAGAATGCGAAGCTAATACGCACAGCGCATGCTAAACCAAAAAGTTATAATGCGTCTAGAGGGAAGAAGCGAAAGAATGACTCTGTCTGTAAGGACAAAGGTGCCATACCGATGGAAGATCTTGTCCTTCAAATGCCACAAACACTAAAGAAACAACTAGTTGATGATTGCGAATTTATTACTCATCTGGGCAAGCTTGTTAAACTTCCACGTACTCCAAATGTGGATGACATATTCAAGAAGTATCTAGATTACAGATCAAAGGACAGTAAGAAGATTGAAGCTCAATCTGTTGAAGAAATCCTGAAAGGACTGTGTTGTTACTTTGACAAAGCACTACCGGCAATGCTTTTGTACAAAAATGAACGTCAGCAGTATGAGAAAGCAATCACAGATGATGTCTCTCCGTCATCTGTCTATGGTGCCGAGCATCTATTACGGCTCTTCGTTAAACTACCCGAGTTATTGTTGGATGCTAACACTGAAGAGGAGACGTCGAAAGCGTTGCAGCAAAAATTGGTCGACTTTCTCAAGTTCCTACAGACGAACCAGAGTGCGTTTTTCCTTTCTACCTATTATGTACCAGACGATATTGAAACCAGCACCAACAAACCAGATGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000123 GO:0003674 GO:0003682 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006325 GO:0006338 GO:0006342 GO:0006355 GO:0006464 GO:0006473 GO:0006475 GO:0006476 GO:0006807 GO:0006996 GO:0008150 GO:0008152 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016043 GO:0016458 GO:0016569 GO:0016570 GO:0016573 GO:0016575 GO:0018193 GO:0018205 GO:0018393 GO:0018394 GO:0019219 GO:0019222 GO:0019538 GO:0031248 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031974 GO:0031981 GO:0032991 GO:0035267 GO:0035601 GO:0036211 GO:0040029 GO:0040034 GO:0043170 GO:0043189 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043543 GO:0043967 GO:0043968 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0045814 GO:0045892 GO:0045934 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0060255 GO:0065007 GO:0070013 GO:0071704 GO:0071840 GO:0080090 GO:0098732 GO:1901564 GO:1902493 GO:1902494 GO:1902562 GO:1902679 GO:1903506 GO:1903507 GO:1990188 GO:1990234 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MRG Tudor-knot

Protein Analysis

333

Amino Acids

38.03

Weight (kDa)

7.58

Isoelectric Point (pI)

43.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MSL3_chromo-like	PF22732	54 - 119	8.9e-14	MSL3 chromodomain-like
Tudor-knot	PF11717	54 - 104	2.8e-12	RNA binding activity-knot of a chromodomain
MRG	PF05712	147 - 318	1.9e-47	MRG

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006520)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02740
fragaria_vesca	FvH4_2g33880 FvH4_2g33880 FvH4_2g33880 FvH4_2g33880 FvH4_2g33880
malus_domestica	MD08G1091800.v1.1 MD12G1169000.v1.1 MD15G1076200.v1.1
prunus_persica	Prupe.1G429500_v2.0.a1
pyrus_communis	pycom08g07450
rosa_chinensis	RchiOBHm_Chr6g0308521 RchiOBHm_Chr6g0308621
rosa_laevigata	RLG00000010635
rosa_multiflora	Rmu_sc0001161.1_g000019
rosa_roxburghii	Rroxscaffold_7G00160050
rosa_rugosa	Rorug06G0367500 Rorug06G0367600
rosa_samantha	Rh6AG477400 Rh6AG477700 Rh6AG478100 Rh6BG487700 Rh6CG492700 Rh6DG478900
rosa_wichuraiana	Rw6G041660 Rw6G041690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	423
AatII	GACGTC	1 cut(s)	872
AccB1I	GGYRCC	2 cut(s)	476, 797
AccI	GTMKAC	1 cut(s)	897
AciI	CCGC	4 cut(s)	32, 63, 66, 84
AclWI	GGATC	2 cut(s)	103, 312
AcsI	RAATTY	1 cut(s)	548
AcuI	CTGAAG	1 cut(s)	879
AcyI	GRCGYC	1 cut(s)	869
AfaI	GTAC	5 cut(s)	206, 350, 589, 731, 957
AgsI	TTSAA	5 cut(s)	506, 613, 656, 671, 971
AhlI	ACTAGT	1 cut(s)	532
AluBI	AGCT	4 cut(s)	81, 388, 571, 659
AluI	AGCT	4 cut(s)	81, 388, 571, 659
Alw26I	GTCTC	2 cut(s)	781, 860
AlwI	GGATC	2 cut(s)	103, 312
Ama87I	CYCGRG	1 cut(s)	833
AoxI	GGCC	2 cut(s)	177, 210
ApeKI	GCWGC	1 cut(s)	883
ApoI	RAATTY	1 cut(s)	548
ArsI	GACNNNNNNTTYG	1 cut(s)	978
AspLEI	GCGC	1 cut(s)	403
AspS9I	GGNCC	1 cut(s)	178
AvaI	CYCGRG	1 cut(s)	833
BaeI	ACNNNNGTAYC	2 cut(s)	332, 365
BanI	GGYRCC	2 cut(s)	476, 797
BbvI	GCAGC	1 cut(s)	895
BccI	CCATC	3 cut(s)	232, 264, 481
BceAI	ACGGC	1 cut(s)	830
BcgI	CGANNNNNNTGC	2 cut(s)	862, 896
BcoDI	GTCTC	2 cut(s)	781, 860
BcuI	ACTAGT	1 cut(s)	532
BfaI	CTAG	3 cut(s)	432, 533, 623
BfmI	CTRYAG	1 cut(s)	914
BglII	AGATCT	1 cut(s)	493
BisI	GCNGC	3 cut(s)	33, 66, 884
BlsI	GCNGC	3 cut(s)	34, 67, 885
BmeT110I	CYCGRG	1 cut(s)	833
BmgT120I	GGNCC	1 cut(s)	178
BmiI	GGNNCC	3 cut(s)	16, 478, 799
BmsI	GCATC	2 cut(s)	814, 838
BpuEI	CTTGAG	1 cut(s)	890
BsaAI	YACGTR	1 cut(s)	587
BsaHI	GRCGYC	1 cut(s)	869
BsaJI	CCNNGG	2 cut(s)	165, 213
Bse118I	RCCGGY	1 cut(s)	715
Bse1I	ACTGG	1 cut(s)	346
Bse3DI	GCAATG	1 cut(s)	726
BseDI	CCNNGG	2 cut(s)	165, 213
BseGI	GGATG	4 cut(s)	275, 292, 607, 853
BseMI	GCAATG	1 cut(s)	726
BseNI	ACTGG	1 cut(s)	346
BseRI	GAGGAG	1 cut(s)	878
BseXI	GCAGC	1 cut(s)	895
BshFI	GGCC	2 cut(s)	179, 212
BshNI	GGYRCC	2 cut(s)	476, 797
BsiHKCI	CYCGRG	1 cut(s)	833
BsiSI	CCGG	1 cut(s)	716
BsmAI	GTCTC	2 cut(s)	781, 860
BsmBI	CGTCTC	1 cut(s)	860
BsmI	GAATGC	1 cut(s)	385
BsnI	GGCC	2 cut(s)	179, 212
BsoBI	CYCGRG	1 cut(s)	833
Bsp1407I	TGTACA	1 cut(s)	729
Bsp143I	GATC	4 cut(s)	108, 304, 493, 632
BspACI	CCGC	4 cut(s)	32, 63, 66, 84
BspANI	GGCC	2 cut(s)	179, 212
BspLI	GGNNCC	3 cut(s)	16, 478, 799
BspPI	GGATC	2 cut(s)	103, 312
BspQI	GCTCTTC	1 cut(s)	824
BspT107I	GGYRCC	2 cut(s)	476, 797
BsrDI	GCAATG	1 cut(s)	726
BsrFI	RCCGGY	1 cut(s)	715
BsrGI	TGTACA	1 cut(s)	729
BsrI	ACTGG	1 cut(s)	346
BssAI	RCCGGY	1 cut(s)	715
BssECI	CCNNGG	2 cut(s)	165, 213
BssMI	GATC	4 cut(s)	108, 304, 493, 632
BssNI	GRCGYC	1 cut(s)	869
BssT1I	CCWWGG	1 cut(s)	213
Bst4CI	ACNGT	3 cut(s)	204, 644, 690
Bst6I	CTCTTC	2 cut(s)	824, 855
BstACI	GRCGYC	1 cut(s)	869
BstAUI	TGTACA	1 cut(s)	729
BstBAI	YACGTR	1 cut(s)	587
BstC8I	GCNNGC	2 cut(s)	405, 569
BstEII	GGTNACC	1 cut(s)	217
BstF5I	GGATG	4 cut(s)	275, 292, 607, 853
BstHHI	GCGC	1 cut(s)	403
BstKTI	GATC	4 cut(s)	111, 307, 496, 635
BstMAI	GTCTC	2 cut(s)	781, 860
BstMBI	GATC	4 cut(s)	108, 304, 493, 632
BstMWI	GCNNNNNNNGC	1 cut(s)	716
BstNSI	RCATGY	1 cut(s)	407
BstPI	GGTNACC	1 cut(s)	217
BstSFI	CTRYAG	1 cut(s)	914
BstV1I	GCAGC	1 cut(s)	895
BstX2I	RGATCY	2 cut(s)	108, 493
BstXI	CCANNNNNNTGG	1 cut(s)	487
BstYI	RGATCY	2 cut(s)	108, 493
BsuRI	GGCC	2 cut(s)	179, 212
BtsCI	GGATG	4 cut(s)	275, 292, 607, 853
BtsIMutI	CAGTG	4 cut(s)	45, 51, 321, 855
Cac8I	GCNNGC	2 cut(s)	405, 569
CfoI	GCGC	1 cut(s)	403
Cfr10I	RCCGGY	1 cut(s)	715
Cfr13I	GGNCC	1 cut(s)	178
CseI	GACGC	3 cut(s)	15, 137, 417
Csp6I	GTAC	5 cut(s)	205, 349, 588, 730, 956
CviAII	CATG	1 cut(s)	404
CviJI	RGCY	8 cut(s)	15, 81, 179, 212, 388, 571, 659, 817
CviKI_1	RGCY	8 cut(s)	15, 81, 179, 212, 388, 571, 659, 817
CviQI	GTAC	5 cut(s)	205, 349, 588, 730, 956
DpnI	GATC	4 cut(s)	110, 306, 495, 634
DpnII	GATC	4 cut(s)	108, 304, 493, 632
Eam1104I	CTCTTC	2 cut(s)	824, 855
EarI	CTCTTC	2 cut(s)	824, 855
EciI	GGCGGA	2 cut(s)	52, 73
Eco130I	CCWWGG	1 cut(s)	213
Eco57I	CTGAAG	1 cut(s)	879
Eco88I	CYCGRG	1 cut(s)	833
Eco91I	GGTNACC	1 cut(s)	217
EcoO109I	RGGNCCY	1 cut(s)	178
EcoO65I	GGTNACC	1 cut(s)	217
EcoT14I	CCWWGG	1 cut(s)	213
ErhI	CCWWGG	1 cut(s)	213
Esp3I	CGTCTC	1 cut(s)	860
FaeI	CATG	1 cut(s)	407
FaiI	YATR	9 cut(s)	234, 255, 405, 423, 482, 608, 753, 795, 954
FatI	CATG	1 cut(s)	403
FblI	GTMKAC	1 cut(s)	897
Fnu4HI	GCNGC	3 cut(s)	33, 66, 884
FokI	GGATG	4 cut(s)	282, 299, 614, 860
Fsp4HI	GCNGC	3 cut(s)	33, 66, 884
FspBI	CTAG	3 cut(s)	432, 533, 623
GlaI	GCGC	1 cut(s)	402
GluI	GCNGC	3 cut(s)	33, 66, 884
HaeIII	GGCC	2 cut(s)	179, 212
HapII	CCGG	1 cut(s)	716
HgaI	GACGC	3 cut(s)	15, 137, 417
HhaI	GCGC	1 cut(s)	403
Hin1I	GRCGYC	1 cut(s)	869
Hin1II	CATG	1 cut(s)	407
Hin6I	GCGC	1 cut(s)	401
HinP1I	GCGC	1 cut(s)	401
HincII	GTYRAC	1 cut(s)	898
HindII	GTYRAC	1 cut(s)	898
HindIII	AAGCTT	1 cut(s)	569
HinfI	GANTC	5 cut(s)	56, 71, 98, 149, 455
HpaII	CCGG	1 cut(s)	716
Hpy166II	GTNNAC	1 cut(s)	898
Hpy188I	TCNGA	4 cut(s)	76, 103, 230, 312
Hpy188III	TCNNGA	4 cut(s)	432, 613, 623, 679
Hpy8I	GTNNAC	1 cut(s)	898
Hpy99I	CGWCG	3 cut(s)	119, 131, 874
HpyAV	CCTTC	3 cut(s)	150, 169, 512
HpyCH4III	ACNGT	3 cut(s)	204, 644, 690
HpyCH4IV	ACGT	3 cut(s)	586, 742, 869
HpyCH4V	TGCA	1 cut(s)	883
HpyF10VI	GCNNNNNNNGC	1 cut(s)	716
HpySE526I	ACGT	3 cut(s)	586, 742, 869
Hsp92I	GRCGYC	1 cut(s)	869
Hsp92II	CATG	1 cut(s)	407
HspAI	GCGC	1 cut(s)	401
Kzo9I	GATC	4 cut(s)	108, 304, 493, 632
LguI	GCTCTTC	1 cut(s)	824
LmnI	GCTCC	2 cut(s)	20, 86
Lsp1109I	GCAGC	1 cut(s)	895
LweI	GCATC	2 cut(s)	814, 838
MaeI	CTAG	3 cut(s)	432, 533, 623
MaeII	ACGT	3 cut(s)	586, 742, 869
MaeIII	GTNAC	2 cut(s)	217, 695
MalI	GATC	4 cut(s)	110, 306, 495, 634
MboI	GATC	4 cut(s)	108, 304, 493, 632
MboII	GAAGA	7 cut(s)	349, 451, 503, 661, 683, 811, 872
MflI	RGATCY	2 cut(s)	108, 493
MluCI	AATT	2 cut(s)	548, 890
MlyI	GAGTC	3 cut(s)	50, 80, 449
MmeI	TCCRAC	4 cut(s)	99, 126, 282, 825
MnlI	CCTC	6 cut(s)	160, 191, 202, 362, 428, 856
MseI	TTAA	2 cut(s)	576, 825
MslI	CAYNNNNRTG	1 cut(s)	485
MspI	CCGG	1 cut(s)	716
Mva1269I	GAATGC	1 cut(s)	385
MwoI	GCNNNNNNNGC	1 cut(s)	716
NdeII	GATC	4 cut(s)	108, 304, 493, 632
NlaIII	CATG	1 cut(s)	407
NlaIV	GGNNCC	3 cut(s)	16, 478, 799
NmeAIII	GCCGAG	2 cut(s)	94, 826
NspI	RCATGY	1 cut(s)	407
PaeI	GCATGC	1 cut(s)	407
PciSI	GCTCTTC	1 cut(s)	824
PcsI	WCGNNNNNNNCGW	2 cut(s)	123, 875
PctI	GAATGC	1 cut(s)	385
PfeI	GAWTC	2 cut(s)	98, 149
PflFI	GACNNNGTC	1 cut(s)	458
PkrI	GCNGC	3 cut(s)	34, 67, 885
PleI	GAGTC	3 cut(s)	50, 79, 449
PpsI	GAGTC	3 cut(s)	50, 79, 449
Ppu21I	YACGTR	1 cut(s)	587
PsiI	TTATAA	1 cut(s)	423
PspEI	GGTNACC	1 cut(s)	217
PspN4I	GGNNCC	3 cut(s)	16, 478, 799
PspPI	GGNCC	1 cut(s)	178
PsuI	RGATCY	2 cut(s)	108, 493
PsyI	GACNNNGTC	1 cut(s)	458
RsaI	GTAC	5 cut(s)	206, 350, 589, 731, 957
RsaNI	GTAC	5 cut(s)	205, 349, 588, 730, 956
RseI	CAYNNNNRTG	1 cut(s)	485
SalI	GTCGAC	1 cut(s)	896
SapI	GCTCTTC	1 cut(s)	824
SaqAI	TTAA	2 cut(s)	576, 825
SatI	GCNGC	3 cut(s)	33, 66, 884
Sau3AI	GATC	4 cut(s)	108, 304, 493, 632
Sau96I	GGNCC	1 cut(s)	178
SchI	GAGTC	3 cut(s)	50, 80, 449
SfaNI	GCATC	2 cut(s)	814, 838
SfcI	CTRYAG	1 cut(s)	914
SmiMI	CAYNNNNRTG	1 cut(s)	485
SmlI	CTYRAG	1 cut(s)	905
SmoI	CTYRAG	1 cut(s)	905
SpeI	ACTAGT	1 cut(s)	532
SphI	GCATGC	1 cut(s)	407
Sse9I	AATT	2 cut(s)	548, 890
SsiI	CCGC	4 cut(s)	32, 63, 66, 84
SspMI	CTAG	3 cut(s)	432, 533, 623
StyI	CCWWGG	1 cut(s)	213
TaaI	ACNGT	3 cut(s)	204, 644, 690
TaiI	ACGT	3 cut(s)	589, 745, 872
TaqI	TCGA	2 cut(s)	872, 897
TaqII	GACCGA	1 cut(s)	110
TasI	AATT	2 cut(s)	548, 890
TatI	WGTACW	1 cut(s)	729
TauI	GCSGC	2 cut(s)	35, 68
TfiI	GAWTC	2 cut(s)	98, 149
Tru1I	TTAA	2 cut(s)	576, 825
Tru9I	TTAA	2 cut(s)	576, 825
TscAI	CASTG	4 cut(s)	52, 58, 328, 862
TseI	GCWGC	1 cut(s)	883
TspDTI	ATGAA	3 cut(s)	248, 329, 753
TspGWI	ACGGA	1 cut(s)	771
TspRI	CASTG	4 cut(s)	52, 58, 328, 862
Tth111I	GACNNNGTC	1 cut(s)	458
XapI	RAATTY	1 cut(s)	548
XbaI	TCTAGA	2 cut(s)	431, 622
XceI	RCATGY	1 cut(s)	407
XmiI	GTMKAC	1 cut(s)	897
XspI	CTAG	3 cut(s)	432, 533, 623
ZraI	GACGTC	1 cut(s)	870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.