Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010818

Physical Location & Seq

Genomic Coordinates Forward (+)

8098188 .. 8101859

Gene Structure 3672 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1114800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1020 bp

ATGCATGCGAAAGCGACGCAATCGTCGTTTCGGGACCGGACGCACGAGTTTCAGAGTGTAGCGGAGAGGCTAAAGAAGTCGTTCTCATCAGCGCCGGGGCAGAATGGGCCGAGTAGTGGATTGAAATCGAAATCTGAGGAGCAGCGTTCCGCGGTTGCAATTCAGTCGGAGTTCAATAAGAGGGCCTCCAAAATCGGGTATGGGATACATCAGACGTCGCAGAAACTTGGAAAGCTGACAAAGTTGGCAAAGAGGACTTCCGTGTTTGATGATCCTACAAGGGAAATTCAGGAGCTGACAGCGGTTATCAAGCAAGATATTACTGCCCTTAACTCTGCAGTGGTGGACCTTCAGCATCTATCTAACTCCAGAAATCAAAGTGGGAATATTTCTAGTGACACGAGTAGTCATTCAACCACAGTTGTTGATGACCTGAAAAATCGGCTGATGAGTGCCACAAAAGAGTTTAAAGAAGTGCTCACCATGCGAACTGAGAATTTGAAGGTTCATGAGAATAGAAGACAATTGTTTTCTGCGACTGCATCTAAGGATGCTGCAAATCCCTTTGTTCGTCAACGTCCACTGGCTGCCAGGTCAGCTGCTAGTTCTTCAAGTGCTTCTCCTCCTCCATGGGTTGGATCACCCTCTTCATCACAGGCGTTCCCCAAGAAGCAGATGGACGGGGAGAGTCAGCCATTGCTCCAGCAGCAGCAGCAACAGGAGCTGGTTCCGGTGCAAGACAGTTACATGCAAAGCAGAGCTGAAGCTTTGCAAAATGTTGAATCCACTATTCATGAGTTGAGCAATATTTTCAACCAGCTTGCGACCTTGGTTTCCCAGCAAGGGGAGGTTGCCATAAGAATTGATGAGAACATGGAAGATACATTAGCGAACGTAGAGGGCGCACAAGGGGCATTGCTCAAGTACCTGAATAGCATATCATCTAACAGGTGGCTGATGATCAAGATATTCTTTATACTAATATTTTTCCTCATGGTTTTCCTATTTTTTGTGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0006888 GO:0006906 GO:0006996 GO:0008150 GO:0009987 GO:0012505 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0031090 GO:0031201 GO:0031224 GO:0031984 GO:0032991 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0048193 GO:0048278 GO:0048284 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0071840 GO:0090174 GO:0098588 GO:0098791 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 map04130

Pfam Domains

Protein Families

SNARE Syntaxin Syntaxin-5_N

Protein Analysis

340

Amino Acids

37.62

Weight (kDa)

9.42

Isoelectric Point (pI)

59.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin-5_N	PF11416	8 - 26	5.6e-06	Syntaxin-5 N-terminal, Sly1p-binding domain
SNARE	PF05739	284 - 335	3.9e-16	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010818)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24350 AT3G24350
fragaria_vesca	FvH4_2g30200
malus_domestica	MD15G1114700.v1.1 MD15G1114800.v1.1 MD15G1190200.v1.1
prunus_persica	Prupe.1G466200_v2.0.a1 Prupe.1G466200_v2.0.a1 Prupe.7G226900_v2.0.a1
pyrus_communis	pycom15g10340
rosa_chinensis	RchiOBHm_Chr6g0312681
rosa_laevigata	RLG00000010304
rosa_multiflora	Rmu_sc0002859.1_g000001 Rmu_ssc0000412.1_g000010
rosa_roxburghii	Rroxscaffold_7G00156570
rosa_samantha	Rh6AG515700 Rh6BG527200 Rh6CG532100 Rh6DG518100
rosa_wichuraiana	Rw6G044810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	22
AatII	GACGTC	1 cut(s)	218
AccB7I	CCANNNNNTGG	1 cut(s)	635
AccII	CGCG	1 cut(s)	152
AciI	CCGC	4 cut(s)	62, 150, 152, 302
AclWI	GGATC	2 cut(s)	266, 646
AcsI	RAATTY	2 cut(s)	285, 496
AcuI	CTGAAG	2 cut(s)	335, 783
AcyI	GRCGYC	1 cut(s)	215
AfaI	GTAC	1 cut(s)	926
AfiI	CCNNNNNNNGG	5 cut(s)	116, 195, 635, 843, 844
AgsI	TTSAA	7 cut(s)	124, 175, 414, 502, 612, 782, 814
AjnI	CCWGG	1 cut(s)	590
AluBI	AGCT	7 cut(s)	235, 295, 599, 724, 761, 767, 820
AluI	AGCT	7 cut(s)	235, 295, 599, 724, 761, 767, 820
Alw21I	GWGCWC	1 cut(s)	480
AlwI	GGATC	2 cut(s)	266, 646
AlwNI	CAGNNNCTG	2 cut(s)	295, 724
AoxI	GGCC	2 cut(s)	107, 183
ApeKI	GCWGC	7 cut(s)	142, 554, 587, 599, 706, 709, 712
ApoI	RAATTY	2 cut(s)	285, 496
Asp700I	GAANNNNTTC	1 cut(s)	80
AspLEI	GCGC	2 cut(s)	94, 905
AspS9I	GGNCC	4 cut(s)	34, 107, 183, 346
AsuC2I	CCSGG	1 cut(s)	96
AsuHPI	GGTGA	2 cut(s)	472, 633
AvaII	GGWCC	2 cut(s)	34, 346
BauI	CACGAG	2 cut(s)	44, 400
BbsI	GAAGAC	1 cut(s)	526
Bbv12I	GWGCWC	1 cut(s)	480
BbvI	GCAGC	7 cut(s)	154, 541, 574, 586, 718, 721, 724
BccI	CCATC	1 cut(s)	670
BcgI	CGANNNNNNTGC	2 cut(s)	147, 181
BciT130I	CCWGG	1 cut(s)	592
BciVI	GTATCC	1 cut(s)	198
BclI	TGATCA	1 cut(s)	960
BcnI	CCSGG	1 cut(s)	96
BfaI	CTAG	2 cut(s)	393, 603
BfmI	CTRYAG	1 cut(s)	336
BfoI	RGCGCY	1 cut(s)	95
BfuI	GTATCC	1 cut(s)	198
BisI	GCNGC	7 cut(s)	143, 555, 588, 600, 707, 710, 713
BlsI	GCNGC	7 cut(s)	144, 556, 589, 601, 708, 711, 714
Bme1390I	CCNGG	2 cut(s)	96, 592
Bme18I	GGWCC	2 cut(s)	34, 346
BmgT120I	GGNCC	4 cut(s)	34, 107, 183, 346
BmiI	GGNNCC	2 cut(s)	35, 729
BmrFI	CCNGG	2 cut(s)	96, 592
BmsI	GCATC	3 cut(s)	364, 541, 551
BpiI	GAAGAC	1 cut(s)	526
BpmI	CTGGAG	2 cut(s)	352, 686
BpuEI	CTTGAG	1 cut(s)	905
BpuMI	CCSGG	1 cut(s)	96
BsaBI	GATNNNNATC	1 cut(s)	124
BsaHI	GRCGYC	1 cut(s)	215
BsaJI	CCNNGG	4 cut(s)	95, 150, 629, 828
BsaWI	WCCGGW	2 cut(s)	36, 730
Bsc4I	CCNNNNNNNGG	5 cut(s)	116, 195, 635, 843, 844
Bse1I	ACTGG	1 cut(s)	588
Bse3DI	GCAATG	2 cut(s)	695, 914
Bse8I	GATNNNNATC	1 cut(s)	124
BseBI	CCWGG	1 cut(s)	592
BseDI	CCNNGG	4 cut(s)	95, 150, 629, 828
BseGI	GGATG	1 cut(s)	556
BseJI	GATNNNNATC	1 cut(s)	124
BseLI	CCNNNNNNNGG	5 cut(s)	116, 195, 635, 843, 844
BseMI	GCAATG	2 cut(s)	695, 914
BseMII	CTCAG	2 cut(s)	126, 483
BseNI	ACTGG	1 cut(s)	588
BseRI	GAGGAG	3 cut(s)	152, 612, 615
BseXI	GCAGC	7 cut(s)	154, 541, 574, 586, 718, 721, 724
BseYI	CCCAGC	1 cut(s)	837
Bsh1236I	CGCG	1 cut(s)	152
BshFI	GGCC	2 cut(s)	109, 185
BsiHKAI	GWGCWC	1 cut(s)	480
BsiSI	CCGG	3 cut(s)	37, 95, 731
BslFI	GGGAC	1 cut(s)	47
BslI	CCNNNNNNNGG	5 cut(s)	116, 195, 635, 843, 844
BsmFI	GGGAC	1 cut(s)	47
BsnI	GGCC	2 cut(s)	109, 185
Bsp1286I	GDGCHC	1 cut(s)	480
Bsp143I	GATC	3 cut(s)	271, 638, 960
Bsp19I	CCATGG	1 cut(s)	629
BspACI	CCGC	4 cut(s)	62, 150, 152, 302
BspANI	GGCC	2 cut(s)	109, 185
BspCNI	CTCAG	2 cut(s)	127, 484
BspFNI	CGCG	1 cut(s)	152
BspHI	TCATGA	2 cut(s)	508, 793
BspLI	GGNNCC	2 cut(s)	35, 729
BspMAI	CTGCAG	1 cut(s)	340
BspPI	GGATC	2 cut(s)	266, 646
BsrDI	GCAATG	2 cut(s)	695, 914
BsrI	ACTGG	1 cut(s)	588
BssECI	CCNNGG	4 cut(s)	95, 150, 629, 828
BssMI	GATC	3 cut(s)	271, 638, 960
BssNI	GRCGYC	1 cut(s)	215
BssSI	CACGAG	2 cut(s)	44, 400
BssT1I	CCWWGG	2 cut(s)	629, 828
Bst2BI	CACGAG	2 cut(s)	44, 400
Bst2UI	CCWGG	1 cut(s)	592
Bst4CI	ACNGT	2 cut(s)	421, 743
Bst6I	CTCTTC	1 cut(s)	652
BstACI	GRCGYC	1 cut(s)	215
BstC8I	GCNNGC	2 cut(s)	6, 822
BstDEI	CTNAG	3 cut(s)	135, 492, 546
BstDSI	CCRYGG	2 cut(s)	150, 629
BstF5I	GGATG	1 cut(s)	556
BstFNI	CGCG	1 cut(s)	152
BstH2I	RGCGCY	1 cut(s)	95
BstHHI	GCGC	2 cut(s)	94, 905
BstKTI	GATC	3 cut(s)	274, 641, 963
BstMBI	GATC	3 cut(s)	271, 638, 960
BstMWI	GCNNNNNNNGC	7 cut(s)	106, 484, 596, 706, 712, 721, 911
BstNI	CCWGG	1 cut(s)	592
BstNSI	RCATGY	2 cut(s)	8, 751
BstSCI	CCNGG	2 cut(s)	94, 590
BstSFI	CTRYAG	1 cut(s)	336
BstUI	CGCG	1 cut(s)	152
BstV1I	GCAGC	7 cut(s)	154, 541, 574, 586, 718, 721, 724
BstV2I	GAAGAC	1 cut(s)	526
BsuI	GTATCC	1 cut(s)	198
BsuRI	GGCC	2 cut(s)	109, 185
BtgI	CCRYGG	2 cut(s)	150, 629
BtsCI	GGATG	1 cut(s)	556
BtsI	GCAGTG	1 cut(s)	345
BtsIMutI	CAGTG	2 cut(s)	345, 581
Cac8I	GCNNGC	2 cut(s)	6, 822
CaiI	CAGNNNCTG	2 cut(s)	295, 724
CciI	TCATGA	2 cut(s)	508, 793
CfoI	GCGC	2 cut(s)	94, 905
Cfr13I	GGNCC	4 cut(s)	34, 107, 183, 346
Cfr42I	CCGCGG	1 cut(s)	153
CseI	GACGC	2 cut(s)	25, 49
Csp6I	GTAC	1 cut(s)	925
CspCI	CAANNNNNGTGG	2 cut(s)	406, 441
CviAII	CATG	8 cut(s)	5, 484, 509, 630, 748, 794, 874, 994
CviQI	GTAC	1 cut(s)	925
DdeI	CTNAG	3 cut(s)	135, 492, 546
DpnI	GATC	3 cut(s)	273, 640, 962
DpnII	GATC	3 cut(s)	271, 638, 960
DraI	TTTAAA	1 cut(s)	469
DrdI	GACNNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	1 cut(s)	22
Eam1104I	CTCTTC	1 cut(s)	652
EarI	CTCTTC	1 cut(s)	652
Eco130I	CCWWGG	2 cut(s)	629, 828
Eco47I	GGWCC	2 cut(s)	34, 346
Eco57I	CTGAAG	2 cut(s)	335, 783
EcoO109I	RGGNCCY	1 cut(s)	183
EcoRII	CCWGG	1 cut(s)	590
EcoT14I	CCWWGG	2 cut(s)	629, 828
EcoT22I	ATGCAT	1 cut(s)	6
ErhI	CCWWGG	2 cut(s)	629, 828
FaeI	CATG	8 cut(s)	8, 487, 512, 633, 751, 797, 877, 997
FalI	AAGNNNNNCTT	2 cut(s)	956, 988
FaqI	GGGAC	1 cut(s)	47
FatI	CATG	8 cut(s)	4, 483, 508, 629, 747, 793, 873, 993
FbaI	TGATCA	1 cut(s)	960
Fnu4HI	GCNGC	7 cut(s)	143, 555, 588, 600, 707, 710, 713
FokI	GGATG	1 cut(s)	563
Fsp4HI	GCNGC	7 cut(s)	143, 555, 588, 600, 707, 710, 713
FspBI	CTAG	2 cut(s)	393, 603
GlaI	GCGC	2 cut(s)	93, 904
GluI	GCNGC	7 cut(s)	143, 555, 588, 600, 707, 710, 713
GsaI	CCCAGC	1 cut(s)	841
GsuI	CTGGAG	2 cut(s)	352, 686
HaeII	RGCGCY	1 cut(s)	95
HaeIII	GGCC	2 cut(s)	109, 185
HapII	CCGG	3 cut(s)	37, 95, 731
HgaI	GACGC	2 cut(s)	25, 49
HhaI	GCGC	2 cut(s)	94, 905
Hin1I	GRCGYC	1 cut(s)	215
Hin1II	CATG	8 cut(s)	8, 487, 512, 633, 751, 797, 877, 997
Hin6I	GCGC	2 cut(s)	92, 903
HinP1I	GCGC	2 cut(s)	92, 903
HincII	GTYRAC	1 cut(s)	575
HindII	GTYRAC	1 cut(s)	575
HindIII	AAGCTT	1 cut(s)	765
HinfI	GANTC	2 cut(s)	688, 782
HpaII	CCGG	3 cut(s)	37, 95, 731
HphI	GGTGA	2 cut(s)	472, 633
Hpy166II	GTNNAC	3 cut(s)	346, 575, 581
Hpy188I	TCNGA	4 cut(s)	54, 136, 169, 213
Hpy188III	TCNNGA	6 cut(s)	32, 290, 369, 509, 794, 964
Hpy8I	GTNNAC	3 cut(s)	346, 575, 581
Hpy99I	CGWCG	3 cut(s)	19, 28, 220
HpyAV	CCTTC	2 cut(s)	359, 496
HpyCH4III	ACNGT	2 cut(s)	421, 743
HpyCH4IV	ACGT	3 cut(s)	215, 577, 894
HpyCH4V	TGCA	8 cut(s)	4, 158, 338, 542, 557, 736, 751, 772
HpyF10VI	GCNNNNNNNGC	7 cut(s)	106, 484, 596, 706, 712, 721, 911
HpyF3I	CTNAG	3 cut(s)	135, 492, 546
HpySE526I	ACGT	3 cut(s)	215, 577, 894
Hsp92I	GRCGYC	1 cut(s)	215
Hsp92II	CATG	8 cut(s)	8, 487, 512, 633, 751, 797, 877, 997
HspAI	GCGC	2 cut(s)	92, 903
Ksp22I	TGATCA	1 cut(s)	960
KspI	CCGCGG	1 cut(s)	153
Kzo9I	GATC	3 cut(s)	271, 638, 960
LmnI	GCTCC	4 cut(s)	139, 292, 705, 721
Lsp1109I	GCAGC	7 cut(s)	154, 541, 574, 586, 718, 721, 724
LweI	GCATC	3 cut(s)	364, 541, 551
MaeI	CTAG	2 cut(s)	393, 603
MaeII	ACGT	3 cut(s)	215, 577, 894
MaeIII	GTNAC	2 cut(s)	395, 743
MalI	GATC	3 cut(s)	273, 640, 962
MboI	GATC	3 cut(s)	271, 638, 960
MboII	GAAGA	4 cut(s)	531, 600, 639, 890
MfeI	CAATTG	1 cut(s)	524
MhlI	GDGCHC	1 cut(s)	480
MluCI	AATT	5 cut(s)	159, 285, 496, 524, 861
MlyI	GAGTC	1 cut(s)	697
MmeI	TCCRAC	2 cut(s)	147, 616
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	1 cut(s)	80
MseI	TTAA	2 cut(s)	330, 468
MspA1I	CMGCKG	3 cut(s)	152, 302, 599
MspI	CCGG	3 cut(s)	37, 95, 731
MspR9I	CCNGG	2 cut(s)	96, 592
MunI	CAATTG	1 cut(s)	524
MvaI	CCWGG	1 cut(s)	592
MvnI	CGCG	1 cut(s)	152
MwoI	GCNNNNNNNGC	7 cut(s)	106, 484, 596, 706, 712, 721, 911
NciI	CCSGG	1 cut(s)	96
NcoI	CCATGG	1 cut(s)	629
NdeII	GATC	3 cut(s)	271, 638, 960
NlaIII	CATG	8 cut(s)	8, 487, 512, 633, 751, 797, 877, 997
NlaIV	GGNNCC	2 cut(s)	35, 729
NmeAIII	GCCGAG	1 cut(s)	135
NmuCI	GTSAC	1 cut(s)	395
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	8, 751
PaeI	GCATGC	1 cut(s)	8
PagI	TCATGA	2 cut(s)	508, 793
PcsI	WCGNNNNNNNCGW	1 cut(s)	23
PdmI	GAANNNNTTC	1 cut(s)	80
PfeI	GAWTC	1 cut(s)	782
PflMI	CCANNNNNTGG	1 cut(s)	635
PkrI	GCNGC	7 cut(s)	144, 556, 589, 601, 708, 711, 714
PleI	GAGTC	1 cut(s)	696
PpsI	GAGTC	1 cut(s)	696
Psp6I	CCWGG	1 cut(s)	590
PspFI	CCCAGC	1 cut(s)	837
PspGI	CCWGG	1 cut(s)	590
PspN4I	GGNNCC	2 cut(s)	35, 729
PspPI	GGNCC	4 cut(s)	34, 107, 183, 346
PstI	CTGCAG	1 cut(s)	340
PstNI	CAGNNNCTG	2 cut(s)	295, 724
PvuII	CAGCTG	1 cut(s)	599
RsaI	GTAC	1 cut(s)	926
RsaNI	GTAC	1 cut(s)	925
SacII	CCGCGG	1 cut(s)	153
SaqAI	TTAA	2 cut(s)	330, 468
SatI	GCNGC	7 cut(s)	143, 555, 588, 600, 707, 710, 713
Sau3AI	GATC	3 cut(s)	271, 638, 960
Sau96I	GGNCC	4 cut(s)	34, 107, 183, 346
SchI	GAGTC	1 cut(s)	697
ScrFI	CCNGG	2 cut(s)	96, 592
SduI	GDGCHC	1 cut(s)	480
SfaNI	GCATC	3 cut(s)	364, 541, 551
SfcI	CTRYAG	1 cut(s)	336
Sfr303I	CCGCGG	1 cut(s)	153
SgrBI	CCGCGG	1 cut(s)	153
SinI	GGWCC	2 cut(s)	34, 346
SmlI	CTYRAG	1 cut(s)	920
SmoI	CTYRAG	1 cut(s)	920
SphI	GCATGC	1 cut(s)	8
Sse9I	AATT	5 cut(s)	159, 285, 496, 524, 861
SsiI	CCGC	4 cut(s)	62, 150, 152, 302
SspI	AATATT	3 cut(s)	388, 808, 984
SspMI	CTAG	2 cut(s)	393, 603
StyD4I	CCNGG	2 cut(s)	94, 590
StyI	CCWWGG	2 cut(s)	629, 828
TaaI	ACNGT	2 cut(s)	421, 743
TaiI	ACGT	3 cut(s)	218, 580, 897
TaqI	TCGA	1 cut(s)	128
TasI	AATT	5 cut(s)	159, 285, 496, 524, 861
TfiI	GAWTC	1 cut(s)	782
Tru1I	TTAA	2 cut(s)	330, 468
Tru9I	TTAA	2 cut(s)	330, 468
TscAI	CASTG	2 cut(s)	345, 588
TseFI	GTSAC	1 cut(s)	395
TseI	GCWGC	7 cut(s)	142, 554, 587, 599, 706, 709, 712
Tsp45I	GTSAC	1 cut(s)	395
TspDTI	ATGAA	3 cut(s)	497, 639, 782
TspGWI	ACGGA	1 cut(s)	250
TspRI	CASTG	2 cut(s)	345, 588
Van91I	CCANNNNNTGG	1 cut(s)	635
VpaK11BI	GGWCC	2 cut(s)	34, 346
XapI	RAATTY	2 cut(s)	285, 496
XceI	RCATGY	2 cut(s)	8, 751
XcmI	CCANNNNNNNNNTGG	1 cut(s)	673
XmnI	GAANNNNTTC	1 cut(s)	80
XspI	CTAG	2 cut(s)	393, 603
ZraI	GACGTC	1 cut(s)	216
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.