MD15G1190200.v1.1

Belongs to the syntaxin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0010818
Physical Location & Seq
Forward (+)
14983742 .. 14986223
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1190200.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGCAAGGGAAATCCGGCCAATCGTCGTTCCGAGATCGAACGCAGGAGTTTCAGAGCGTCACGGATAGGCTTCGTAAGTCGTTCGCGCCATCTTTCAATGGGCCGTCGAGCAGCGGCAGCAGTGGTGGGAAGCTCGACGGACCTCGATCTGCGGCGTCGGTTCAATCGGAGTTCAACAAAAGGGCTTCCAAGATTGGGCTCGGGATTCACCACACTTCCCAGAAGCTTGGAAAGCTCGCACAATTGGCGAAAAGGACATCGGTGTTTGATGATCCCGCTCTGGAGATCCAAGAGCTGACAGCAGCTATAAAACAAGACATTACTGGACTTAATTCAGCACTGATAGAGCTGCAGCTGCACTGCAATTCCCAGAACGAAAGTGGGAACATCTCGAGTGACACCACGACTCATTCAACCACCGTTGTAGATAACCTGAAGAACCGGTTGATGAGTGCTACGAAGGAGTTTAAGGAAGTTCTGACCATGCGGACAGAGAATTTAAAGGTTCATGAAAATAGGAGGCAGTTATTTTCTTCAACCACTTCGAAAGAGTCAACAAATCCGTTTGTTCGTCAACGTCCTGTGGCTGCCAGATCAGCTGCTAATGCATCGACAGCTCCTCCTCCATGGGCCAATGATTCTGCATCTTCGTCACAGTTATTTCCTAGGAAGCAAACAGATAAGGAATCTCGACCATTATTGCAGCAGCAGCAGCAACAGGTAGTAGAACAACAAGACACCTATATGCATAGCCGCTCTGAGGCTCTTCAGAACGTGGAGTCAACTATTCACGAGCTGGGTAACATATTTACCCAATTGGCAACCATGGTTTCGCAGCAAGGAGAGCTTGCAATCAGGATTGACGAGAGCATGGACGAGACGCTGTCCAACGTGGAAGGAGCGCAAGGGCAGCTGGCCAGGTACCTTAACAGTATATCATCCAACCGATGGCTAATGATGAAGATATTCTTTGTACTTATTCTATTTCTTATATTTTTTGTATTTTTTGCGGCATAA

Protein Analysis

339

Amino Acids

37.51

Weight (kDa)

9.16

Isoelectric Point (pI)

57.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNARE PF05739 283 - 334 5e-16 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 921
AccB1I GGYRCC 1 cut(s) 921
AccB7I CCANNNNNTGG 1 cut(s) 948
AccBSI CCGCTC 2 cut(s) 278, 756
AccII CGCG 1 cut(s) 86
AciI CCGC 6 cut(s) 114, 152, 276, 487, 754, 1010
AclWI GGATC 2 cut(s) 266, 280
AcoI YGGCCR 2 cut(s) 16, 915
AcsI RAATTY 1 cut(s) 496
AcuI CTGAAG 2 cut(s) 455, 752
AcyI GRCGYC 1 cut(s) 155
AfaI GTAC 2 cut(s) 923, 975
AfiI CCNNNNNNNGG 3 cut(s) 195, 760, 948
AgeI ACCGGT 1 cut(s) 441
AgsI TTSAA 5 cut(s) 97, 164, 175, 414, 537
AjnI CCWGG 1 cut(s) 917
Alw26I GTCTC 1 cut(s) 872
AlwI GGATC 2 cut(s) 266, 280
Ama87I CYCGRG 2 cut(s) 200, 391
AoxI GGCC 4 cut(s) 16, 101, 630, 915
ApoI RAATTY 1 cut(s) 496
AsiGI ACCGGT 1 cut(s) 441
Asp700I GAANNNNTTC 1 cut(s) 965
Asp718I GGTACC 1 cut(s) 921
AspA2I CCTAGG 1 cut(s) 665
AspLEI GCGC 2 cut(s) 88, 904
AspS9I GGNCC 3 cut(s) 101, 140, 630
AsuHPI GGTGA 1 cut(s) 200
AsuII TTCGAA 1 cut(s) 545
AvaI CYCGRG 2 cut(s) 200, 391
AvaII GGWCC 1 cut(s) 140
AvrII CCTAGG 1 cut(s) 665
BalI TGGCCA 1 cut(s) 917
BanI GGYRCC 1 cut(s) 921
BanII GRGCYC 1 cut(s) 201
BauI CACGAG 1 cut(s) 791
BccI CCATC 2 cut(s) 97, 942
BceAI ACGGC 1 cut(s) 88
BciT130I CCWGG 1 cut(s) 919
BcoDI GTCTC 1 cut(s) 872
BfaI CTAG 1 cut(s) 666
BfmI CTRYAG 1 cut(s) 350
BlnI CCTAGG 1 cut(s) 665
Bme1390I CCNGG 1 cut(s) 919
Bme18I GGWCC 1 cut(s) 140
BmeT110I CYCGRG 2 cut(s) 200, 391
BmgT120I GGNCC 3 cut(s) 101, 140, 630
BmiI GGNNCC 1 cut(s) 923
BmrFI CCNGG 1 cut(s) 919
BmsI GCATC 2 cut(s) 617, 653
BpmI CTGGAG 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 545
BsaHI GRCGYC 1 cut(s) 155
BsaJI CCNNGG 3 cut(s) 626, 665, 825
BsaWI WCCGGW 1 cut(s) 441
BsaXI ACNNNNNCTCC 2 cut(s) 604, 634
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 760, 948
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 1 cut(s) 328
BseBI CCWGG 1 cut(s) 919
BseDI CCNNGG 3 cut(s) 626, 665, 825
BseGI GGATG 1 cut(s) 938
BseLI CCNNNNNNNGG 3 cut(s) 195, 760, 948
BseMII CTCAG 1 cut(s) 750
BseNI ACTGG 1 cut(s) 328
BseRI GAGGAG 2 cut(s) 609, 612
BseYI CCCAGC 1 cut(s) 796
BsgI GTGCAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 86
BshFI GGCC 4 cut(s) 18, 103, 632, 917
BshNI GGYRCC 1 cut(s) 921
BshTI ACCGGT 1 cut(s) 441
BsiHKCI CYCGRG 2 cut(s) 200, 391
BsiSI CCGG 2 cut(s) 15, 442
BslI CCNNNNNNNGG 3 cut(s) 195, 760, 948
BsmAI GTCTC 1 cut(s) 872
BsmBI CGTCTC 1 cut(s) 872
BsnI GGCC 4 cut(s) 18, 103, 632, 917
BsoBI CYCGRG 2 cut(s) 200, 391
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 5 cut(s) 34, 146, 271, 285, 593
Bsp19I CCATGG 2 cut(s) 626, 825
BspACI CCGC 6 cut(s) 114, 152, 276, 487, 754, 1010
BspANI GGCC 4 cut(s) 18, 103, 632, 917
BspCNI CTCAG 1 cut(s) 751
BspFNI CGCG 1 cut(s) 86
BspHI TCATGA 1 cut(s) 508
BspLI GGNNCC 1 cut(s) 923
BspMAI CTGCAG 1 cut(s) 354
BspPI GGATC 2 cut(s) 266, 280
BspQI GCTCTTC 1 cut(s) 771
BspT104I TTCGAA 1 cut(s) 545
BspT107I GGYRCC 1 cut(s) 921
BsrBI CCGCTC 2 cut(s) 278, 756
BsrFI RCCGGY 1 cut(s) 441
BsrI ACTGG 1 cut(s) 328
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 3 cut(s) 626, 665, 825
BssMI GATC 5 cut(s) 34, 146, 271, 285, 593
BssNI GRCGYC 1 cut(s) 155
BssSI CACGAG 1 cut(s) 791
BssT1I CCWWGG 3 cut(s) 626, 665, 825
Bst2BI CACGAG 1 cut(s) 791
Bst2UI CCWGG 1 cut(s) 919
Bst4CI ACNGT 3 cut(s) 421, 657, 932
Bst6I CTCTTC 1 cut(s) 771
BstACI GRCGYC 1 cut(s) 155
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 237, 849, 915
BstDEI CTNAG 1 cut(s) 759
BstDSI CCRYGG 2 cut(s) 626, 825
BstF5I GGATG 1 cut(s) 938
BstFNI CGCG 1 cut(s) 86
BstHHI GCGC 2 cut(s) 88, 904
BstKTI GATC 5 cut(s) 37, 149, 274, 288, 596
BstMAI GTCTC 1 cut(s) 872
BstMBI GATC 5 cut(s) 34, 146, 271, 285, 593
BstNI CCWGG 1 cut(s) 919
BstSCI CCNGG 1 cut(s) 917
BstSFI CTRYAG 1 cut(s) 350
BstUI CGCG 1 cut(s) 86
BstX2I RGATCY 1 cut(s) 285
BstXI CCANNNNNNTGG 1 cut(s) 227
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 4 cut(s) 18, 103, 632, 917
BtgI CCRYGG 2 cut(s) 626, 825
BtsCI GGATG 1 cut(s) 938
BtsI GCAGTG 2 cut(s) 127, 358
BtsIMutI CAGTG 3 cut(s) 127, 338, 358
Cac8I GCNNGC 3 cut(s) 237, 849, 915
CciI TCATGA 1 cut(s) 508
CfoI GCGC 2 cut(s) 88, 904
Cfr10I RCCGGY 1 cut(s) 441
Cfr13I GGNCC 3 cut(s) 101, 140, 630
CseI GACGC 3 cut(s) 46, 144, 889
Csp6I GTAC 2 cut(s) 922, 974
CspAI ACCGGT 1 cut(s) 441
CviAII CATG 5 cut(s) 484, 509, 627, 826, 871
CviQI GTAC 2 cut(s) 922, 974
DdeI CTNAG 1 cut(s) 759
DpnI GATC 5 cut(s) 36, 148, 273, 287, 595
DpnII GATC 5 cut(s) 34, 146, 271, 285, 593
DraI TTTAAA 1 cut(s) 501
EaeI YGGCCR 2 cut(s) 16, 915
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Eco130I CCWWGG 3 cut(s) 626, 665, 825
Eco24I GRGCYC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 140
Eco57I CTGAAG 2 cut(s) 455, 752
Eco88I CYCGRG 2 cut(s) 200, 391
EcoRII CCWGG 1 cut(s) 917
EcoT14I CCWWGG 3 cut(s) 626, 665, 825
EcoT22I ATGCAT 2 cut(s) 610, 750
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 3 cut(s) 626, 665, 825
Esp3I CGTCTC 1 cut(s) 872
FaeI CATG 5 cut(s) 487, 512, 630, 829, 874
FalI AAGNNNNNCTT 4 cut(s) 831, 863, 953, 985
FatI CATG 5 cut(s) 483, 508, 626, 825, 870
FauI CCCGC 1 cut(s) 283
FokI GGATG 1 cut(s) 925
FriOI GRGCYC 1 cut(s) 201
FspBI CTAG 1 cut(s) 666
GlaI GCGC 2 cut(s) 87, 903
GsaI CCCAGC 1 cut(s) 800
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 4 cut(s) 18, 103, 632, 917
HapII CCGG 2 cut(s) 15, 442
HgaI GACGC 3 cut(s) 46, 144, 889
HhaI GCGC 2 cut(s) 88, 904
Hin1I GRCGYC 1 cut(s) 155
Hin1II CATG 5 cut(s) 487, 512, 630, 829, 874
Hin6I GCGC 2 cut(s) 86, 902
HinP1I GCGC 2 cut(s) 86, 902
HincII GTYRAC 3 cut(s) 555, 575, 783
HindII GTYRAC 3 cut(s) 555, 575, 783
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 6 cut(s) 205, 406, 551, 638, 686, 779
HpaII CCGG 2 cut(s) 15, 442
HphI GGTGA 1 cut(s) 200
Hpy166II GTNNAC 3 cut(s) 555, 575, 783
Hpy188I TCNGA 6 cut(s) 32, 54, 169, 480, 760, 771
Hpy188III TCNNGA 7 cut(s) 202, 281, 391, 509, 690, 791, 856
Hpy8I GTNNAC 3 cut(s) 555, 575, 783
Hpy99I CGWCG 4 cut(s) 28, 109, 140, 160
HpyAV CCTTC 2 cut(s) 454, 890
HpyCH4III ACNGT 3 cut(s) 421, 657, 932
HpyCH4IV ACGT 3 cut(s) 577, 774, 891
HpyCH4V TGCA 9 cut(s) 4, 352, 358, 363, 608, 644, 703, 748, 851
HpyF3I CTNAG 1 cut(s) 759
HpySE526I ACGT 3 cut(s) 577, 774, 891
Hsp92I GRCGYC 1 cut(s) 155
Hsp92II CATG 5 cut(s) 487, 512, 630, 829, 874
HspAI GCGC 2 cut(s) 86, 902
KpnI GGTACC 1 cut(s) 925
Kzo9I GATC 5 cut(s) 34, 146, 271, 285, 593
LguI GCTCTTC 1 cut(s) 771
LmnI GCTCC 2 cut(s) 622, 899
LweI GCATC 2 cut(s) 617, 653
MaeI CTAG 1 cut(s) 666
MaeII ACGT 3 cut(s) 577, 774, 891
MaeIII GTNAC 4 cut(s) 58, 395, 651, 800
MalI GATC 5 cut(s) 36, 148, 273, 287, 595
MbiI CCGCTC 2 cut(s) 278, 756
MboI GATC 5 cut(s) 34, 146, 271, 285, 593
MboII GAAGA 5 cut(s) 448, 525, 639, 758, 973
MfeI CAATTG 2 cut(s) 242, 815
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 201
MlsI TGGCCA 1 cut(s) 917
MluCI AATT 5 cut(s) 242, 331, 364, 496, 815
MluNI TGGCCA 1 cut(s) 917
MlyI GAGTC 3 cut(s) 400, 560, 788
MmeI TCCRAC 2 cut(s) 912, 966
MnlI CCTC 5 cut(s) 153, 513, 630, 633, 754
Mox20I TGGCCA 1 cut(s) 917
Mph1103I ATGCAT 2 cut(s) 610, 750
MroXI GAANNNNTTC 1 cut(s) 965
MscI TGGCCA 1 cut(s) 917
MseI TTAA 4 cut(s) 330, 468, 500, 927
MslI CAYNNNNRTG 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 917
MspA1I CMGCKG 4 cut(s) 114, 355, 599, 913
MspI CCGG 2 cut(s) 15, 442
MspR9I CCNGG 1 cut(s) 919
MunI CAATTG 2 cut(s) 242, 815
MvaI CCWGG 1 cut(s) 919
MvnI CGCG 1 cut(s) 86
NcoI CCATGG 2 cut(s) 626, 825
NdeII GATC 5 cut(s) 34, 146, 271, 285, 593
NlaIII CATG 5 cut(s) 487, 512, 630, 829, 874
NlaIV GGNNCC 1 cut(s) 923
NmuCI GTSAC 3 cut(s) 58, 395, 651
NsiI ATGCAT 2 cut(s) 610, 750
NspV TTCGAA 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 391
PagI TCATGA 1 cut(s) 508
PciSI GCTCTTC 1 cut(s) 771
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 3 cut(s) 205, 638, 686
PflFI GACNNNGTC 1 cut(s) 883
PflMI CCANNNNNTGG 1 cut(s) 948
PinAI ACCGGT 1 cut(s) 441
PleI GAGTC 3 cut(s) 400, 559, 787
PpsI GAGTC 3 cut(s) 400, 559, 787
Psp6I CCWGG 1 cut(s) 917
PspFI CCCAGC 1 cut(s) 796
PspGI CCWGG 1 cut(s) 917
PspN4I GGNNCC 1 cut(s) 923
PspPI GGNCC 3 cut(s) 101, 140, 630
PstI CTGCAG 1 cut(s) 354
PsuI RGATCY 1 cut(s) 285
PsyI GACNNNGTC 1 cut(s) 883
PvuII CAGCTG 3 cut(s) 355, 599, 913
RsaI GTAC 2 cut(s) 923, 975
RsaNI GTAC 2 cut(s) 922, 974
RseI CAYNNNNRTG 1 cut(s) 743
SapI GCTCTTC 1 cut(s) 771
SaqAI TTAA 4 cut(s) 330, 468, 500, 927
Sau3AI GATC 5 cut(s) 34, 146, 271, 285, 593
Sau96I GGNCC 3 cut(s) 101, 140, 630
SchI GAGTC 3 cut(s) 400, 560, 788
ScrFI CCNGG 1 cut(s) 919
SduI GDGCHC 1 cut(s) 201
SfaNI GCATC 2 cut(s) 617, 653
SfcI CTRYAG 1 cut(s) 350
Sfr274I CTCGAG 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 545
SinI GGWCC 1 cut(s) 140
SlaI CTCGAG 1 cut(s) 391
SmiMI CAYNNNNRTG 1 cut(s) 743
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 5 cut(s) 242, 331, 364, 496, 815
SsiI CCGC 6 cut(s) 114, 152, 276, 487, 754, 1010
SspMI CTAG 1 cut(s) 666
StyD4I CCNGG 1 cut(s) 917
StyI CCWWGG 3 cut(s) 626, 665, 825
TaaI ACNGT 3 cut(s) 421, 657, 932
TaiI ACGT 3 cut(s) 580, 777, 894
TaqI TCGA 8 cut(s) 37, 107, 135, 145, 392, 545, 611, 691
TasI AATT 5 cut(s) 242, 331, 364, 496, 815
TatI WGTACW 1 cut(s) 973
TauI GCSGC 4 cut(s) 117, 155, 756, 1013
TfiI GAWTC 3 cut(s) 205, 638, 686
Tru1I TTAA 4 cut(s) 330, 468, 500, 927
Tru9I TTAA 4 cut(s) 330, 468, 500, 927
TscAI CASTG 3 cut(s) 127, 345, 365
TseFI GTSAC 3 cut(s) 58, 395, 651
Tsp45I GTSAC 3 cut(s) 58, 395, 651
TspDTI ATGAA 3 cut(s) 497, 525, 974
TspGWI ACGGA 3 cut(s) 77, 153, 552
TspRI CASTG 3 cut(s) 127, 345, 365
Tth111I GACNNNGTC 1 cut(s) 883
Van91I CCANNNNNTGG 1 cut(s) 948
VpaK11BI GGWCC 1 cut(s) 140
XapI RAATTY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
XhoI CTCGAG 1 cut(s) 391
XmaJI CCTAGG 1 cut(s) 665
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 1 cut(s) 666
Zsp2I ATGCAT 2 cut(s) 610, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.