Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014668

Physical Location & Seq

Genomic Coordinates Forward (+)

9075667 .. 9078479

Gene Structure 2813 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1125000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2424 bp

ATGCTTAATTTCTCTTTTGCTCAAGTGTTCTTACCTGATATTTTTTCTTCCTTTACTTTTGACATTGTGGATGCCCCAAAGTCACCTAAAAGGTTTGCAACTCAAATCAAGAAGTTGGTGCTTTGCCGACCCCAGCAGATTGTTGGATCCAAACTCTATGCAACAATTGATCTAGACAAGGCGAGGGTCGGCCGGACGCGAATGGTAAATGATCCAAACAATCCGAAATGGAGGGAAGAATTTTACATCTACTGTGCCCACAACATCTCACAAATTATATTCACTGTCAAAGATGATGATCTCATTGGGGCAACCCTAATTGGAAGAGCATATATACCAGTTGGGGACATAATCAAGGGTTATGTGGAGGAAAGATGGGTTGAAATTTTGGATGAAGATCACAATCCCATCCATGGTAATTCTAGAATTCATGTCAAGTTACAATTTTCCAGTGTTGCAGAAGATTTTCATTGGTCACTTGGGATTAGAAACCCTAAGTATGAAGGTGTTCCTCGGACGTTTTTCAATCAAAGGGAGGGTTGTAGGGTTACTCTATACCAAGATTCGCATGTTGCAGATGAATTTAATCCTCATATTCCTACGCCCCTAGGCGGATTATATGAGCCGCAGAGATGTTGGGAGGACATCTTTGATGCAATCACTAATGCAAGGCACTTTATTTACATAACTGGATGGTCTGTGTACACTAAAATAACCTTGTTAAGGGACCCAAGAAGGCGAAAAGATGACATCACACTAGGGCAGCTTCTTAAGAGGAAGGCTGATGAGGGCGTTTTGGTTCTTTTGCTTGTTTGGGATGATAGGACTTCTATTGAGGAGTTGAAGAAGGATGGTTTGATGACAACTCATGATCAAGAAACCGAAGAATATTTTCGAAACACAAAAGTGCATTGCTTCTTATGCCCTCGGAACCCTGATGTAGGGAAAAGCATAATTCAGGGTTTTCAGACTGCCACGATGTTTACTCACCATCAGAAAACGATTGTGTTGGACAGTGAGATATCAGGCAGAGGATCACAAAAGAGGAGGATTGTGAGCTTTGTCGGAGGCATTGATCTTTGTGATGGAAGATATGATACTCAAGAACATCCCTTGTTTGCAACCCTAGGCACAACCCACCAGAATGATTTCCATCAGCCCAACTTTGCAGGCTCTTCAATTAGGAAAGGTGGTCCAAGGGAGCCCTGGCATGACATTCATTGCAAACTGGAAGGGCCGGTTGCTTGGGATGTCTTATACAACTTTGAGCAGAGGTGGAAAAAACAGGTTGGAGAGAAGTTCTTAATTCCACTAGGCAAACTTGAAGAAATCACAGTTCACCCTTTACAAGATATGTTTTCGAACGACGACGAATCATGGAACGTTCAGCTATTCAGGTCCATTGACAATGGGGCTGCTTTTGGCTTCCCTGAAAAGCCTTCTGAAGCAACACAAATAGGACTTGTGAGTGGGAAGAATAACATCATCGACCGAAGCATTCAAGACGCGTATATCCATGCCATTCGCCGAGCCAAAAACTTCATTTACATTGAGAATCAGTATTTTCTTGGAAGTTCATTTGGATGGAGATCAAAAGACATTAGAGATGCGGATATCAATGCTTTACACCTTATACCGAAGGAGCTTTCGCTAAAGATTGTTAGCAAAATTGAAGTCGGAGAGAGGTTCAGCGTCTACATTGTTGTCCCAATGTGGCCAGAAGGTATGCCTGAGAGTGCTTCTGTTCAGGCTATATTGGATTGGCAAAGAAGGACAATGGAGATGATGTACACTGATATCTCAGAAGCCCTTCAGAAAAAGGGCTCAAATGCAAACCCTAGGGATTATCTCACATTCTTCTGCCTCGGCAACCGGGAGCGGGAGATGGATGGAGAATATAAACCTCCCGAGACTCCAGAGCCAGACACCGATTACAAAAGAGCTCAGAAGGCCCGGCGTTTCATGATTTACGTGCATTCAAAAATGATGATAGTTGATGATGAATACATAATCATTGGATCTGCCAACATAAACCAAAGGTCCATGGATGGGGCAAGGGACTCTGAGATTGCTATAGGAGCATTCCAACCTAACCATTTAGCTTCGAAAATCCCAGCCAAGGGCCAGATCCATGCTTTCAGGCTAGCACTATGGTATGAGCACCTCAAAGTGCCTGACGACTCCTTCGTGCATCCAGAATCTTTGGAATGCATCCGAAGAGTGAACCAGATTGCCGAAAGAAACTGGGAATTTTACTCGAGCGACACGATTGATGACATTCCAGGTCACCTTCTCCACTACCCCATTGCGGTAAGCAATAATGGAGCTCTAACAACACTGCCTGGCTTCGAATATTTTCCCGATACCAAGGCTCGTGTTTTCGGCGCCAAATCTGACTACCTTCCTCCAATCCTCACCACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004620 GO:0004630 GO:0006629 GO:0006643 GO:0006950 GO:0006970 GO:0007275 GO:0008081 GO:0008150 GO:0008152 GO:0008610 GO:0009056 GO:0009058 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0009987 GO:0010033 GO:0010035 GO:0016042 GO:0016298 GO:0016787 GO:0016788 GO:0022622 GO:0032501 GO:0032502 GO:0033993 GO:0042221 GO:0042578 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0046466 GO:0048364 GO:0048731 GO:0048856 GO:0050896 GO:0071704 GO:0097305 GO:0099402 GO:1901575 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00564 ko00565 ko01100 ko01110 ko04014 ko04024 ko04071 ko04072 ko04144 ko04666 ko04724 ko04912 ko05231 map00564 map00565 map01100 map01110 map04014 map04024 map04071 map04072 map04144 map04666 map04724 map04912 map05231

Pfam Domains

Protein Families

C2 PLD_C PLDc

Protein Analysis

808

Amino Acids

92.86

Weight (kDa)

6.16

Isoelectric Point (pI)

39.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	50 - 128	2e-10	C2 domain
PLDc	PF00614	656 - 681	3.4e-08	Phospholipase D Active site motif
PLDc_2	PF13091	656 - 690	2.5e-06	PLD-like domain
PLD_C	PF12357	726 - 797	3.5e-29	Phospholipase D C terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014668)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g29120
malus_domestica	MD15G1125000.v1.1
prunus_persica	Prupe.1G480500_v2.0.a1 Prupe.1G480500_v2.0.a1
pyrus_communis	pycom15g11260
rosa_chinensis	RchiOBHm_Chr6g0298001
rosa_laevigata	RLG00000011544
rosa_multiflora	Rmu_sc0002800.1_g000023
rosa_roxburghii	Rroxscaffold_7G00170010 Rroxscaffold_7G00178840
rosa_rugosa	Rorug06G0277800 Rorug06G0277900
rosa_samantha	Rh6AG388200 Rh6BG396400 Rh6CG402400 Rh6DG388600
rosa_wichuraiana	Rw6G033880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	2384
AccBSI	CCGCTC	1 cut(s)	1879
AccI	GTMKAC	1 cut(s)	1695
AccII	CGCG	2 cut(s)	199, 1508
AciI	CCGC	5 cut(s)	612, 626, 1610, 1879, 2309
AclI	AACGTT	1 cut(s)	1383
AclWI	GGATC	6 cut(s)	141, 154, 206, 1042, 2026, 2122
AcoI	YGGCCR	2 cut(s)	190, 1715
AcsI	RAATTY	5 cut(s)	239, 384, 426, 581, 2249
AcuI	CTGAAG	2 cut(s)	1464, 1796
AcyI	GRCGYC	1 cut(s)	2385
AfaI	GTAC	2 cut(s)	704, 1790
AfiI	CCNNNNNNNGG	9 cut(s)	413, 611, 723, 941, 1879, 2049, 2119, 2120, 2308
AflII	CTTAAG	1 cut(s)	770
AflIII	ACRYGT	1 cut(s)	1506
AgsI	TTSAA	8 cut(s)	383, 526, 844, 1179, 1325, 1502, 1673, 1980
AjnI	CCWGG	3 cut(s)	1205, 2281, 2341
AleI	CACNNNNGTG	1 cut(s)	905
AluBI	AGCT	7 cut(s)	766, 1059, 1390, 1645, 1943, 2102, 2327
AluI	AGCT	7 cut(s)	766, 1059, 1390, 1645, 1943, 2102, 2327
Alw21I	GWGCWC	3 cut(s)	1945, 2163, 2329
Alw26I	GTCTC	1 cut(s)	1904
AlwI	GGATC	6 cut(s)	141, 154, 206, 1042, 2026, 2122
Ama87I	CYCGRG	2 cut(s)	1907, 2257
AoxI	GGCC	5 cut(s)	190, 1235, 1715, 1950, 2122
ApeKI	GCWGC	2 cut(s)	763, 1415
ApoI	RAATTY	5 cut(s)	239, 384, 426, 581, 2249
ArsI	GACNNNNNNTTYG	4 cut(s)	1659, 1691, 2168, 2200
Asp700I	GAANNNNTTC	6 cut(s)	465, 507, 891, 1148, 1809, 2355
AspA2I	CCTAGG	3 cut(s)	607, 1126, 1838
AspLEI	GCGC	1 cut(s)	2387
AspS9I	GGNCC	7 cut(s)	727, 1193, 1235, 1398, 1951, 2040, 2122
AsuC2I	CCSGG	2 cut(s)	1874, 1954
AsuHPI	GGTGA	5 cut(s)	75, 980, 1331, 2279, 2407
AsuII	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
AsuNHI	GCTAGC	1 cut(s)	2143
AvaI	CYCGRG	2 cut(s)	1907, 2257
AvaII	GGWCC	4 cut(s)	727, 1193, 1398, 2040
AvrII	CCTAGG	3 cut(s)	607, 1126, 1838
BaeGI	GKGCMC	1 cut(s)	259
BalI	TGGCCA	1 cut(s)	1717
BamHI	GGATCC	1 cut(s)	146
BanI	GGYRCC	1 cut(s)	2384
BanII	GRGCYC	4 cut(s)	1206, 1826, 1945, 2329
BarI	GAAGNNNNNNTAC	2 cut(s)	1081, 1113
BauI	CACGAG	1 cut(s)	2373
Bbv12I	GWGCWC	3 cut(s)	1945, 2163, 2329
BbvI	GCAGC	2 cut(s)	775, 1402
BciT130I	CCWGG	3 cut(s)	1207, 2283, 2343
BclI	TGATCA	1 cut(s)	871
BcnI	CCSGG	2 cut(s)	1874, 1954
BcoDI	GTCTC	1 cut(s)	1904
BfaI	CTAG	8 cut(s)	173, 423, 608, 758, 1127, 1313, 1839, 2144
BfmI	CTRYAG	1 cut(s)	2073
BfoI	RGCGCY	1 cut(s)	2388
BfrI	CTTAAG	1 cut(s)	770
BisI	GCNGC	3 cut(s)	626, 764, 1416
BlnI	CCTAGG	3 cut(s)	607, 1126, 1838
BlsI	GCNGC	3 cut(s)	627, 765, 1417
Bme1390I	CCNGG	5 cut(s)	1207, 1874, 1954, 2283, 2343
Bme18I	GGWCC	4 cut(s)	727, 1193, 1398, 2040
BmeT110I	CYCGRG	2 cut(s)	1907, 2257
BmgT120I	GGNCC	7 cut(s)	727, 1193, 1235, 1398, 1951, 2040, 2122
BmiI	GGNNCC	6 cut(s)	148, 728, 729, 932, 1203, 2386
BmrFI	CCNGG	5 cut(s)	1207, 1874, 1954, 2283, 2343
BmrI	ACTGGG	1 cut(s)	2254
BmsI	GCATC	5 cut(s)	61, 643, 1597, 2200, 2220
BmtI	GCTAGC	1 cut(s)	2147
BmuI	ACTGGG	1 cut(s)	2254
BpmI	CTGGAG	1 cut(s)	1899
Bpu14I	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
BpuEI	CTTGAG	2 cut(s)	6, 1086
BpuMI	CCSGG	2 cut(s)	1874, 1954
BsaAI	YACGTR	1 cut(s)	1972
BsaBI	GATNNNNATC	5 cut(s)	297, 396, 402, 1152, 1588
BsaHI	GRCGYC	1 cut(s)	2385
BsaXI	ACNNNNNCTCC	2 cut(s)	1772, 1802
Bsc4I	CCNNNNNNNGG	9 cut(s)	413, 611, 723, 941, 1879, 2049, 2119, 2120, 2308
Bse118I	RCCGGY	1 cut(s)	1237
Bse1I	ACTGG	5 cut(s)	338, 450, 694, 1233, 2249
Bse3DI	GCAATG	3 cut(s)	910, 1219, 2304
Bse8I	GATNNNNATC	5 cut(s)	297, 396, 402, 1152, 1588
BseBI	CCWGG	3 cut(s)	1207, 2283, 2343
BseJI	GATNNNNATC	5 cut(s)	297, 396, 402, 1152, 1588
BseLI	CCNNNNNNNGG	9 cut(s)	413, 611, 723, 941, 1879, 2049, 2119, 2120, 2308
BseMI	GCAATG	3 cut(s)	910, 1219, 2304
BseMII	CTCAG	4 cut(s)	1722, 1815, 1958, 2055
BseNI	ACTGG	5 cut(s)	338, 450, 694, 1233, 2249
BseRI	GAGGAG	2 cut(s)	851, 1060
BseSI	GKGCMC	1 cut(s)	259
BseX3I	CGGCCG	1 cut(s)	190
BseXI	GCAGC	2 cut(s)	775, 1402
BseYI	CCCAGC	2 cut(s)	132, 2113
Bsh1236I	CGCG	2 cut(s)	199, 1508
Bsh1285I	CGRYCG	2 cut(s)	193, 1492
BshFI	GGCC	5 cut(s)	192, 1237, 1717, 1952, 2124
BshNI	GGYRCC	1 cut(s)	2384
BsiEI	CGRYCG	2 cut(s)	193, 1492
BsiHKAI	GWGCWC	3 cut(s)	1945, 2163, 2329
BsiHKCI	CYCGRG	2 cut(s)	1907, 2257
BsiSI	CCGG	4 cut(s)	193, 1238, 1873, 1954
BslFI	GGGAC	4 cut(s)	359, 740, 1691, 2072
BslI	CCNNNNNNNGG	9 cut(s)	413, 611, 723, 941, 1879, 2049, 2119, 2120, 2308
BsmAI	GTCTC	1 cut(s)	1904
BsmFI	GGGAC	4 cut(s)	359, 740, 1691, 2072
BsmI	GAATGC	4 cut(s)	1497, 1975, 2081, 2213
BsnI	GGCC	5 cut(s)	192, 1237, 1717, 1952, 2124
BsoBI	CYCGRG	2 cut(s)	1907, 2257
Bsp119I	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
Bsp1286I	GDGCHC	6 cut(s)	259, 1206, 1826, 1945, 2163, 2329
Bsp1407I	TGTACA	2 cut(s)	702, 1788
Bsp19I	CCATGG	2 cut(s)	412, 2043
BspACI	CCGC	5 cut(s)	612, 626, 1610, 1879, 2309
BspANI	GGCC	5 cut(s)	192, 1237, 1717, 1952, 2124
BspCNI	CTCAG	4 cut(s)	1723, 1814, 1957, 2056
BspFNI	CGCG	2 cut(s)	199, 1508
BspHI	TCATGA	2 cut(s)	868, 1962
BspLI	GGNNCC	6 cut(s)	148, 728, 729, 932, 1203, 2386
BspOI	GCTAGC	1 cut(s)	2147
BspPI	GGATC	6 cut(s)	141, 154, 206, 1042, 2026, 2122
BspQI	GCTCTTC	2 cut(s)	319, 1180
BspT104I	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
BspT107I	GGYRCC	1 cut(s)	2384
BspTI	CTTAAG	1 cut(s)	770
BsrBI	CCGCTC	1 cut(s)	1879
BsrDI	GCAATG	3 cut(s)	910, 1219, 2304
BsrFI	RCCGGY	1 cut(s)	1237
BsrGI	TGTACA	2 cut(s)	702, 1788
BsrI	ACTGG	5 cut(s)	338, 450, 694, 1233, 2249
BssAI	RCCGGY	1 cut(s)	1237
BssNI	GRCGYC	1 cut(s)	2385
BssSI	CACGAG	1 cut(s)	2373
BssT1I	CCWWGG	8 cut(s)	412, 607, 1126, 1196, 1838, 2043, 2118, 2367
Bst2BI	CACGAG	1 cut(s)	2373
Bst2UI	CCWGG	3 cut(s)	1207, 2283, 2343
Bst4CI	ACNGT	4 cut(s)	254, 286, 1016, 1336
Bst6I	CTCTTC	3 cut(s)	319, 1180, 2212
BstACI	GRCGYC	1 cut(s)	2385
BstAFI	CTTAAG	1 cut(s)	770
BstAUI	TGTACA	2 cut(s)	702, 1788
BstBAI	YACGTR	1 cut(s)	1972
BstBI	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
BstC8I	GCNNGC	2 cut(s)	1171, 2145
BstDEI	CTNAG	6 cut(s)	495, 1731, 1801, 1944, 2064, 2421
BstDSI	CCRYGG	2 cut(s)	412, 2043
BstEII	GGTNACC	1 cut(s)	2285
BstENI	CCTNNNNNAGG	2 cut(s)	721, 939
BstFNI	CGCG	2 cut(s)	199, 1508
BstH2I	RGCGCY	1 cut(s)	2388
BstHHI	GCGC	1 cut(s)	2387
BstMAI	GTCTC	1 cut(s)	1904
BstMCI	CGRYCG	2 cut(s)	193, 1492
BstMWI	GCNNNNNNNGC	3 cut(s)	921, 1949, 2078
BstNI	CCWGG	3 cut(s)	1207, 2283, 2343
BstNSI	RCATGY	1 cut(s)	572
BstPI	GGTNACC	1 cut(s)	2285
BstSCI	CCNGG	5 cut(s)	1205, 1872, 1952, 2281, 2341
BstSFI	CTRYAG	1 cut(s)	2073
BstSLI	GKGCMC	1 cut(s)	259
BstUI	CGCG	2 cut(s)	199, 1508
BstV1I	GCAGC	2 cut(s)	775, 1402
BstX2I	RGATCY	3 cut(s)	146, 2018, 2127
BstYI	RGATCY	3 cut(s)	146, 2018, 2127
BstZI	CGGCCG	1 cut(s)	190
BsuRI	GGCC	5 cut(s)	192, 1237, 1717, 1952, 2124
BtgI	CCRYGG	2 cut(s)	412, 2043
BtsI	GCAGTG	1 cut(s)	2336
BtsIMutI	CAGTG	5 cut(s)	282, 457, 1021, 1791, 2336
Cac8I	GCNNGC	2 cut(s)	1171, 2145
CciI	TCATGA	2 cut(s)	868, 1962
CfoI	GCGC	1 cut(s)	2387
Cfr10I	RCCGGY	1 cut(s)	1237
Cfr13I	GGNCC	7 cut(s)	727, 1193, 1235, 1398, 1951, 2040, 2122
CseI	GACGC	3 cut(s)	205, 1514, 1681
Csp6I	GTAC	2 cut(s)	703, 1789
CviQI	GTAC	2 cut(s)	703, 1789
DdeI	CTNAG	6 cut(s)	495, 1731, 1801, 1944, 2064, 2421
DinI	GGCGCC	1 cut(s)	2386
EaeI	YGGCCR	2 cut(s)	190, 1715
EagI	CGGCCG	1 cut(s)	190
Eam1104I	CTCTTC	3 cut(s)	319, 1180, 2212
EarI	CTCTTC	3 cut(s)	319, 1180, 2212
EciI	GGCGGA	1 cut(s)	627
Ecl136II	GAGCTC	2 cut(s)	1943, 2327
EclXI	CGGCCG	1 cut(s)	190
Eco130I	CCWWGG	8 cut(s)	412, 607, 1126, 1196, 1838, 2043, 2118, 2367
Eco24I	GRGCYC	4 cut(s)	1206, 1826, 1945, 2329
Eco32I	GATATC	3 cut(s)	1023, 1615, 1798
Eco47I	GGWCC	4 cut(s)	727, 1193, 1398, 2040
Eco52I	CGGCCG	1 cut(s)	190
Eco53kI	GAGCTC	2 cut(s)	1943, 2327
Eco57I	CTGAAG	2 cut(s)	1464, 1796
Eco88I	CYCGRG	2 cut(s)	1907, 2257
Eco91I	GGTNACC	1 cut(s)	2285
EcoICRI	GAGCTC	2 cut(s)	1943, 2327
EcoNI	CCTNNNNNAGG	2 cut(s)	721, 939
EcoO109I	RGGNCCY	1 cut(s)	727
EcoO65I	GGTNACC	1 cut(s)	2285
EcoRI	GAATTC	1 cut(s)	426
EcoRII	CCWGG	3 cut(s)	1205, 2281, 2341
EcoRV	GATATC	3 cut(s)	1023, 1615, 1798
EcoT14I	CCWWGG	8 cut(s)	412, 607, 1126, 1196, 1838, 2043, 2118, 2367
EcoT22I	ATGCAT	1 cut(s)	2213
EcoT38I	GRGCYC	4 cut(s)	1206, 1826, 1945, 2329
EgeI	GGCGCC	1 cut(s)	2386
EheI	GGCGCC	1 cut(s)	2386
ErhI	CCWWGG	8 cut(s)	412, 607, 1126, 1196, 1838, 2043, 2118, 2367
FaqI	GGGAC	4 cut(s)	359, 740, 1691, 2072
FauI	CCCGC	1 cut(s)	1872
FbaI	TGATCA	1 cut(s)	871
FblI	GTMKAC	1 cut(s)	1695
Fnu4HI	GCNGC	3 cut(s)	626, 764, 1416
FriOI	GRGCYC	4 cut(s)	1206, 1826, 1945, 2329
Fsp4HI	GCNGC	3 cut(s)	626, 764, 1416
FspBI	CTAG	8 cut(s)	173, 423, 608, 758, 1127, 1313, 1839, 2144
GlaI	GCGC	1 cut(s)	2386
GluI	GCNGC	3 cut(s)	626, 764, 1416
GsaI	CCCAGC	2 cut(s)	136, 2117
GsuI	CTGGAG	1 cut(s)	1899
HaeII	RGCGCY	1 cut(s)	2388
HaeIII	GGCC	5 cut(s)	192, 1237, 1717, 1952, 2124
HapII	CCGG	4 cut(s)	193, 1238, 1873, 1954
HgaI	GACGC	3 cut(s)	205, 1514, 1681
HhaI	GCGC	1 cut(s)	2387
Hin1I	GRCGYC	1 cut(s)	2385
Hin6I	GCGC	1 cut(s)	2385
HinP1I	GCGC	1 cut(s)	2385
HinfI	GANTC	7 cut(s)	563, 1373, 1555, 1912, 2060, 2180, 2198
HpaII	CCGG	4 cut(s)	193, 1238, 1873, 1954
HphI	GGTGA	5 cut(s)	75, 980, 1331, 2279, 2407
Hpy166II	GTNNAC	7 cut(s)	703, 705, 984, 1339, 1696, 1791, 2224
Hpy8I	GTNNAC	7 cut(s)	703, 705, 984, 1339, 1696, 1791, 2224
Hpy99I	CGWCG	2 cut(s)	1370, 1373
HpyCH4III	ACNGT	4 cut(s)	254, 286, 1016, 1336
HpyCH4IV	ACGT	3 cut(s)	518, 1383, 1971
HpyF10VI	GCNNNNNNNGC	3 cut(s)	921, 1949, 2078
HpyF3I	CTNAG	6 cut(s)	495, 1731, 1801, 1944, 2064, 2421
HpySE526I	ACGT	3 cut(s)	518, 1383, 1971
Hsp92I	GRCGYC	1 cut(s)	2385
HspAI	GCGC	1 cut(s)	2385
KasI	GGCGCC	1 cut(s)	2384
KflI	GGGWCCC	1 cut(s)	727
Ksp22I	TGATCA	1 cut(s)	871
LguI	GCTCTTC	2 cut(s)	319, 1180
LmnI	GCTCC	5 cut(s)	1201, 1642, 1876, 2078, 2324
Lsp1109I	GCAGC	2 cut(s)	775, 1402
LweI	GCATC	5 cut(s)	61, 643, 1597, 2200, 2220
MaeI	CTAG	8 cut(s)	173, 423, 608, 758, 1127, 1313, 1839, 2144
MaeII	ACGT	3 cut(s)	518, 1383, 1971
MaeIII	GTNAC	5 cut(s)	81, 438, 474, 547, 2285
MbiI	CCGCTC	1 cut(s)	1879
MfeI	CAATTG	1 cut(s)	165
MflI	RGATCY	3 cut(s)	146, 2018, 2127
MhlI	GDGCHC	6 cut(s)	259, 1206, 1826, 1945, 2163, 2329
MlsI	TGGCCA	1 cut(s)	1717
MluI	ACGCGT	1 cut(s)	1506
MluNI	TGGCCA	1 cut(s)	1717
Mly113I	GGCGCC	1 cut(s)	2385
MlyI	GAGTC	3 cut(s)	1906, 2054, 2174
MmeI	TCCRAC	6 cut(s)	124, 990, 1045, 1270, 1657, 2110
Mox20I	TGGCCA	1 cut(s)	1717
Mph1103I	ATGCAT	1 cut(s)	2213
MroXI	GAANNNNTTC	6 cut(s)	465, 507, 891, 1148, 1809, 2355
MscI	TGGCCA	1 cut(s)	1717
MseI	TTAA	5 cut(s)	6, 585, 722, 771, 1304
MslI	CAYNNNNRTG	3 cut(s)	905, 1143, 1582
Msp20I	TGGCCA	1 cut(s)	1717
MspCI	CTTAAG	1 cut(s)	770
MspI	CCGG	4 cut(s)	193, 1238, 1873, 1954
MspR9I	CCNGG	5 cut(s)	1207, 1874, 1954, 2283, 2343
MunI	CAATTG	1 cut(s)	165
Mva1269I	GAATGC	4 cut(s)	1497, 1975, 2081, 2213
MvaI	CCWGG	3 cut(s)	1207, 2283, 2343
MvnI	CGCG	2 cut(s)	199, 1508
MwoI	GCNNNNNNNGC	3 cut(s)	921, 1949, 2078
NarI	GGCGCC	1 cut(s)	2385
NciI	CCSGG	2 cut(s)	1874, 1954
NcoI	CCATGG	2 cut(s)	412, 2043
NheI	GCTAGC	1 cut(s)	2143
NlaIV	GGNNCC	6 cut(s)	148, 728, 729, 932, 1203, 2386
NmeAIII	GCCGAG	2 cut(s)	1553, 1845
NmuCI	GTSAC	3 cut(s)	81, 474, 2285
NsiI	ATGCAT	1 cut(s)	2213
NspI	RCATGY	1 cut(s)	572
NspV	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
OliI	CACNNNNGTG	1 cut(s)	905
PaeR7I	CTCGAG	1 cut(s)	2257
PagI	TCATGA	2 cut(s)	868, 1962
PciSI	GCTCTTC	2 cut(s)	319, 1180
PcsI	WCGNNNNNNNCGW	2 cut(s)	2184, 2264
PctI	GAATGC	4 cut(s)	1497, 1975, 2081, 2213
PdmI	GAANNNNTTC	6 cut(s)	465, 507, 891, 1148, 1809, 2355
PfeI	GAWTC	4 cut(s)	563, 1373, 1555, 2198
PkrI	GCNGC	3 cut(s)	627, 765, 1417
PleI	GAGTC	3 cut(s)	1906, 2054, 2174
PluTI	GGCGCC	1 cut(s)	2388
PpsI	GAGTC	3 cut(s)	1906, 2054, 2174
Ppu21I	YACGTR	1 cut(s)	1972
PpuMI	RGGWCCY	1 cut(s)	727
Psp124BI	GAGCTC	2 cut(s)	1945, 2329
Psp1406I	AACGTT	1 cut(s)	1383
Psp5II	RGGWCCY	1 cut(s)	727
Psp6I	CCWGG	3 cut(s)	1205, 2281, 2341
PspEI	GGTNACC	1 cut(s)	2285
PspFI	CCCAGC	2 cut(s)	132, 2113
PspGI	CCWGG	3 cut(s)	1205, 2281, 2341
PspN4I	GGNNCC	6 cut(s)	148, 728, 729, 932, 1203, 2386
PspPI	GGNCC	7 cut(s)	727, 1193, 1235, 1398, 1951, 2040, 2122
PspPPI	RGGWCCY	1 cut(s)	727
PspXI	VCTCGAGB	1 cut(s)	2257
PsuI	RGATCY	3 cut(s)	146, 2018, 2127
RsaI	GTAC	2 cut(s)	704, 1790
RsaNI	GTAC	2 cut(s)	703, 1789
RseI	CAYNNNNRTG	3 cut(s)	905, 1143, 1582
SacI	GAGCTC	2 cut(s)	1945, 2329
SapI	GCTCTTC	2 cut(s)	319, 1180
SaqAI	TTAA	5 cut(s)	6, 585, 722, 771, 1304
SatI	GCNGC	3 cut(s)	626, 764, 1416
Sau96I	GGNCC	7 cut(s)	727, 1193, 1235, 1398, 1951, 2040, 2122
SchI	GAGTC	3 cut(s)	1906, 2054, 2174
ScrFI	CCNGG	5 cut(s)	1207, 1874, 1954, 2283, 2343
SduI	GDGCHC	6 cut(s)	259, 1206, 1826, 1945, 2163, 2329
SfaNI	GCATC	5 cut(s)	61, 643, 1597, 2200, 2220
SfcI	CTRYAG	1 cut(s)	2073
SfoI	GGCGCC	1 cut(s)	2386
Sfr274I	CTCGAG	1 cut(s)	2257
SfuI	TTCGAA	4 cut(s)	895, 1361, 2105, 2349
SinI	GGWCC	4 cut(s)	727, 1193, 1398, 2040
SlaI	CTCGAG	1 cut(s)	2257
SmiMI	CAYNNNNRTG	3 cut(s)	905, 1143, 1582
SmlI	CTYRAG	4 cut(s)	21, 770, 1101, 2257
SmoI	CTYRAG	4 cut(s)	21, 770, 1101, 2257
SsiI	CCGC	5 cut(s)	612, 626, 1610, 1879, 2309
SspDI	GGCGCC	1 cut(s)	2384
SspI	AATATT	2 cut(s)	890, 2354
SspMI	CTAG	8 cut(s)	173, 423, 608, 758, 1127, 1313, 1839, 2144
SstI	GAGCTC	2 cut(s)	1945, 2329
StyD4I	CCNGG	5 cut(s)	1205, 1872, 1952, 2281, 2341
StyI	CCWWGG	8 cut(s)	412, 607, 1126, 1196, 1838, 2043, 2118, 2367
TaaI	ACNGT	4 cut(s)	254, 286, 1016, 1336
TaiI	ACGT	3 cut(s)	521, 1386, 1974
TaqI	TCGA	6 cut(s)	895, 1361, 1488, 2105, 2258, 2349
TaqII	GACCGA	1 cut(s)	1506
TatI	WGTACW	2 cut(s)	702, 1788
TauI	GCSGC	1 cut(s)	628
TfiI	GAWTC	4 cut(s)	563, 1373, 1555, 2198
Tru1I	TTAA	5 cut(s)	6, 585, 722, 771, 1304
Tru9I	TTAA	5 cut(s)	6, 585, 722, 771, 1304
TscAI	CASTG	5 cut(s)	289, 457, 1021, 1798, 2343
TseFI	GTSAC	3 cut(s)	81, 474, 2285
TseI	GCWGC	2 cut(s)	763, 1415
Tsp45I	GTSAC	3 cut(s)	81, 474, 2285
TspRI	CASTG	5 cut(s)	289, 457, 1021, 1798, 2343
Vha464I	CTTAAG	1 cut(s)	770
VpaK11BI	GGWCC	4 cut(s)	727, 1193, 1398, 2040
XagI	CCTNNNNNAGG	2 cut(s)	721, 939
XapI	RAATTY	5 cut(s)	239, 384, 426, 581, 2249
XbaI	TCTAGA	2 cut(s)	172, 422
XceI	RCATGY	1 cut(s)	572
XcmI	CCANNNNNNNNNTGG	2 cut(s)	140, 1203
XhoI	CTCGAG	1 cut(s)	2257
XmaJI	CCTAGG	3 cut(s)	607, 1126, 1838
XmiI	GTMKAC	1 cut(s)	1695
XmnI	GAANNNNTTC	6 cut(s)	465, 507, 891, 1148, 1809, 2355
XspI	CTAG	8 cut(s)	173, 423, 608, 758, 1127, 1313, 1839, 2144
Zsp2I	ATGCAT	1 cut(s)	2213

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.