MD15G1132400.v1.1

PHD-finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006788
Physical Location & Seq
Reverse (-)
9609238 .. 9614423
5186 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1132400.v1.1.491

Sequence Viewer

Length: 1800 bp
ATGGGAGTTGAAGAAGGTAAAAGCAATGGTGGTAGTATGGAATGCGAGAAGGAAGTTGAATGCTTGAAGAGTGAATCCAATGGATTTGGTTTTGGGAATTCTACTGACATTGCCAAAGGCAGCTCTGGCGCAAGTGAGGGTGTTCGAACTTACAAGAGGCGGAGGCAGGCGAGGTTCAGTTGGGATAGCAAGTCACAGGATGGTGGGGGAGCTGATGTGGAATCATCGAGTCAATTGGCAAACCAGCGTCTAAAGGAACCAGTGGGCACAGATATACACAGTAACTCTTGTGAGCAAGTCCATGTTCGAATGAACAGTTCAGATGCATGCTCAGAGAGGAACTGTACAGAAGCTGTGCTGGAGAACATGTATCAGTCATTAGGCGATGATGAAGGTGGTGTACAAGTGTGCATCCGAGAAGCACTTGCACATTTTCGAGAGATTGATCACACAACGCGAGTTAAGGAATCTGGTGATCATGAGGATAGGCACCGATGCTGTTTTCTGACACAGTCTATATTGAATGGATCTCAAAAGGCAGCCAGCATGAATGCTGGTGTTGTATCGAATGGATTTTCAAATAAATCAAACCATCATACAGTTACAGCGATGTGTCAGCGTGCTTTTTATAATGTTTTAGTTTCCGAAACGTTCACCTCATTGTGCAAAGTCCTGCTGCAAAATTTTCAAGGAATCAAGGCTGACAGCGTTTTCGACTTTAGTCTCATAAACTCAAGGATGAAAGGGGGAGATTATGAGCATTCTCCAATGCTCTTCTCAAATGATATGCAACAGGTATGGAGAAAACTTCAAGTGATCGGTACGGACTTGATTTCTCTTGCAAGAAACCTCTCGGACATGTCTAGGGCTTCTTACAATGAACAGGTGGGAGGTTCAATCCGCAATACATTGAAATATGGAAAAAATGAGTTCTACCACTTGGGATCTGACCCTGATGCTAAACTGGAGCAAACAGAGGATTGTGGTGTGCACAGTGCATGCACTTGCAGGTGCTGCGGATGCCATGCAAATGGGAAGGATTGTTTAGTATGTGATTCATGTGAGGAGATGTACCATATCTCTTGCATTGAGCCTGCTGTCAAAGAAATTCCTCCAAGTTGGTATTGTAGTAGGTGCACTGCAAGTGGTATTGGATCACCCCATGAGAACTGTGTCGTGTGTGAGAAGTTGAACGTGGCCAAGACTGTAGTTGATGGAGTTGGAGGTGAAAGTGTTTCTACAGAAGAAGAAACAATCAATGAGACAGGTGAAAATTCAAAATGGAGTGCAGATGATGGAATCCAGCTATCGGAAGAAAGCAAAACAATTTGCAAAACTTGTGGACTTGAGGTAAAAAACAATGATAGTTTGAGGACATGTGGTCATCCATACTGCCCCAAGAAATACTACCATGTGAGGTGTCTGACAACTAACCAGTTAAGATCTTACGGCCCCTGTTGGTACTGCTATTCTTGCCTATGCAGAAATTGTCTCATTGATAAAGATGATGATATGATTGTGCTTTGTGACGGTTGTGATCACGGGTACCACCTCTATTGCATGGAACCACCACGCACTTCCATTCCAGCTGGAAAATGGTTTTGCAAAAAATGTGATACCGGAATTAAGGCCATACGCAAGGCAAGAAAGGCTTATGATAAGAAAGAAAAGACACAGAGAGCGAACTGTGAAGGTTTAAAGAAGTTGAAGGAAAAAGGGGATGACAGGGAATCAGAACAAGGTAGAGGGGGAATGGAGGTCCTTCTCTGTGCAGTCCATACTCTAGATAATGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PHD

Protein Analysis

600

Amino Acids

66.36

Weight (kDa)

5.81

Isoelectric Point (pI)

45.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PHD PF00628 494 - 538 2.1e-10 PHD-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 630
AarI CACCTGC 1 cut(s) 999
Acc36I ACCTGC 1 cut(s) 999
Acc65I GGTACC 1 cut(s) 1545
AccB1I GGYRCC 2 cut(s) 489, 1545
AccII CGCG 1 cut(s) 457
AciI CCGC 3 cut(s) 160, 901, 1017
AclI AACGTT 1 cut(s) 650
AclWI GGATC 3 cut(s) 535, 952, 1162
AcoI YGGCCR 1 cut(s) 1197
AcsI RAATTY 4 cut(s) 97, 682, 1107, 1273
AfaI GTAC 6 cut(s) 346, 402, 823, 1073, 1463, 1547
AfiI CCNNNNNNNGG 1 cut(s) 1309
AflIII ACRYGT 3 cut(s) 366, 858, 1376
AhdI GACNNNNNGTC 1 cut(s) 1380
AluBI AGCT 5 cut(s) 123, 212, 353, 1306, 1589
AluI AGCT 5 cut(s) 123, 212, 353, 1306, 1589
Alw21I GWGCWC 2 cut(s) 993, 1139
Alw26I GTCTC 3 cut(s) 728, 1256, 1496
Alw44I GTGCAC 2 cut(s) 989, 1135
AlwI GGATC 3 cut(s) 535, 952, 1162
AlwNI CAGNNNCTG 2 cut(s) 353, 1014
AoxI GGCC 3 cut(s) 1197, 1450, 1629
ApaLI GTGCAC 2 cut(s) 989, 1135
ApeKI GCWGC 4 cut(s) 120, 539, 676, 1014
ApoI RAATTY 4 cut(s) 97, 682, 1107, 1273
ArsI GACNNNNNNTTYG 2 cut(s) 695, 727
Asp718I GGTACC 1 cut(s) 1545
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 1451, 1759
AsuHPI GGTGA 5 cut(s) 485, 646, 1149, 1238, 1280
AsuII TTCGAA 2 cut(s) 145, 307
AvaII GGWCC 1 cut(s) 1759
BaeGI GKGCMC 3 cut(s) 269, 993, 1139
BalI TGGCCA 1 cut(s) 1199
BanI GGYRCC 2 cut(s) 489, 1545
BarI GAAGNNNNNNTAC 2 cut(s) 384, 416
Bbv12I GWGCWC 2 cut(s) 993, 1139
BbvI GCAGC 4 cut(s) 132, 551, 663, 1001
BccI CCATC 4 cut(s) 194, 600, 1208, 1289
BceAI ACGGC 1 cut(s) 1465
BclI TGATCA 3 cut(s) 445, 475, 1537
BcoDI GTCTC 3 cut(s) 728, 1256, 1496
BfaI CTAG 2 cut(s) 864, 1784
BfmI CTRYAG 2 cut(s) 1206, 1239
BfuAI ACCTGC 1 cut(s) 999
BglII AGATCT 1 cut(s) 1442
BisI GCNGC 4 cut(s) 121, 540, 677, 1015
BlsI GCNGC 4 cut(s) 122, 541, 678, 1016
Bme18I GGWCC 1 cut(s) 1759
BmeRI GACNNNNNGTC 1 cut(s) 1380
BmgT120I GGNCC 2 cut(s) 1451, 1759
BmiI GGNNCC 5 cut(s) 258, 491, 1453, 1547, 1566
BmsI GCATC 5 cut(s) 313, 420, 485, 946, 1010
BoxI GACNNNNGTC 1 cut(s) 720
BpmI CTGGAG 2 cut(s) 380, 986
Bpu14I TTCGAA 2 cut(s) 145, 307
BpuEI CTTGAG 2 cut(s) 718, 1367
BsaWI WCCGGW 1 cut(s) 1619
BsaXI ACNNNNNCTCC 4 cut(s) 201, 231, 353, 383
Bsc4I CCNNNNNNNGG 1 cut(s) 1309
Bse1I ACTGG 3 cut(s) 260, 969, 1435
Bse3DI GCAATG 2 cut(s) 31, 108
BseGI GGATG 6 cut(s) 205, 411, 744, 1025, 1384, 1726
BseLI CCNNNNNNNGG 1 cut(s) 1309
BseMI GCAATG 2 cut(s) 31, 108
BseMII CTCAG 1 cut(s) 345
BseNI ACTGG 3 cut(s) 260, 969, 1435
BseRI GAGGAG 1 cut(s) 1079
BseSI GKGCMC 3 cut(s) 269, 993, 1139
BseXI GCAGC 4 cut(s) 132, 551, 663, 1001
BsgI GTGCAG 2 cut(s) 1308, 1791
Bsh1236I CGCG 1 cut(s) 457
BshFI GGCC 3 cut(s) 1199, 1452, 1631
BshNI GGYRCC 2 cut(s) 489, 1545
BsiHKAI GWGCWC 2 cut(s) 993, 1139
BsiSI CCGG 1 cut(s) 1620
BslI CCNNNNNNNGG 1 cut(s) 1309
BsmAI GTCTC 3 cut(s) 728, 1256, 1496
BsmI GAATGC 4 cut(s) 47, 65, 556, 760
BsnI GGCC 3 cut(s) 1199, 1452, 1631
Bsp119I TTCGAA 2 cut(s) 145, 307
Bsp1286I GDGCHC 3 cut(s) 269, 993, 1139
Bsp1407I TGTACA 2 cut(s) 344, 400
Bsp143I GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
BspACI CCGC 3 cut(s) 160, 901, 1017
BspANI GGCC 3 cut(s) 1199, 1452, 1631
BspCNI CTCAG 1 cut(s) 344
BspFNI CGCG 1 cut(s) 457
BspHI TCATGA 1 cut(s) 478
BspLI GGNNCC 5 cut(s) 258, 491, 1453, 1547, 1566
BspMI ACCTGC 1 cut(s) 999
BspPI GGATC 3 cut(s) 535, 952, 1162
BspQI GCTCTTC 1 cut(s) 779
BspT104I TTCGAA 2 cut(s) 145, 307
BspT107I GGYRCC 2 cut(s) 489, 1545
BsrDI GCAATG 2 cut(s) 31, 108
BsrGI TGTACA 2 cut(s) 344, 400
BsrI ACTGG 3 cut(s) 260, 969, 1435
BssMI GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
Bst6I CTCTTC 3 cut(s) 62, 779, 1788
BstAPI GCANNNNNTGC 1 cut(s) 1014
BstAUI TGTACA 2 cut(s) 344, 400
BstBI TTCGAA 2 cut(s) 145, 307
BstC8I GCNNGC 6 cut(s) 168, 328, 544, 621, 1000, 1095
BstDEI CTNAG 1 cut(s) 331
BstF5I GGATG 6 cut(s) 205, 411, 744, 1025, 1384, 1726
BstFNI CGCG 1 cut(s) 457
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 8 cut(s) 448, 478, 530, 819, 947, 1157, 1445, 1540
BstMAI GTCTC 3 cut(s) 728, 1256, 1496
BstMBI GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
BstMWI GCNNNNNNNGC 5 cut(s) 126, 1014, 1020, 1473, 1649
BstNSI RCATGY 5 cut(s) 330, 370, 862, 1002, 1380
BstPAI GACNNNNGTC 1 cut(s) 720
BstSFI CTRYAG 2 cut(s) 1206, 1239
BstSLI GKGCMC 3 cut(s) 269, 993, 1139
BstUI CGCG 1 cut(s) 457
BstV1I GCAGC 4 cut(s) 132, 551, 663, 1001
BstX2I RGATCY 3 cut(s) 527, 944, 1442
BstXI CCANNNNNNTGG 1 cut(s) 1031
BstYI RGATCY 3 cut(s) 527, 944, 1442
BsuRI GGCC 3 cut(s) 1199, 1452, 1631
BtgZI GCGATG 2 cut(s) 399, 623
BtsCI GGATG 6 cut(s) 205, 411, 744, 1025, 1384, 1726
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 3 cut(s) 267, 1000, 1137
BveI ACCTGC 1 cut(s) 999
Cac8I GCNNGC 6 cut(s) 168, 328, 544, 621, 1000, 1095
CaiI CAGNNNCTG 2 cut(s) 353, 1014
CciI TCATGA 1 cut(s) 478
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 1451, 1759
CseI GACGC 1 cut(s) 236
Csp6I GTAC 6 cut(s) 345, 401, 822, 1072, 1462, 1546
CspCI CAANNNNNGTGG 4 cut(s) 1321, 1356, 1538, 1573
CviQI GTAC 6 cut(s) 345, 401, 822, 1072, 1462, 1546
DdeI CTNAG 1 cut(s) 331
DpnI GATC 8 cut(s) 447, 477, 529, 818, 946, 1156, 1444, 1539
DpnII GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
DraI TTTAAA 1 cut(s) 1698
DriI GACNNNNNGTC 1 cut(s) 1380
EaeI YGGCCR 1 cut(s) 1197
Eam1104I CTCTTC 3 cut(s) 62, 779, 1788
Eam1105I GACNNNNNGTC 1 cut(s) 1380
EarI CTCTTC 3 cut(s) 62, 779, 1788
EciI GGCGGA 1 cut(s) 175
Eco47I GGWCC 1 cut(s) 1759
EcoO109I RGGNCCY 1 cut(s) 1759
EcoRI GAATTC 1 cut(s) 97
EcoT22I ATGCAT 1 cut(s) 328
FalI AAGNNNNNCTT 2 cut(s) 1636, 1668
FbaI TGATCA 3 cut(s) 445, 475, 1537
Fnu4HI GCNGC 4 cut(s) 121, 540, 677, 1015
FokI GGATG 6 cut(s) 212, 398, 751, 1032, 1371, 1733
Fsp4HI GCNGC 4 cut(s) 121, 540, 677, 1015
FspBI CTAG 2 cut(s) 864, 1784
GlaI GCGC 1 cut(s) 130
GluI GCNGC 4 cut(s) 121, 540, 677, 1015
GsuI CTGGAG 2 cut(s) 380, 986
HaeIII GGCC 3 cut(s) 1199, 1452, 1631
HapII CCGG 1 cut(s) 1620
HgaI GACGC 1 cut(s) 236
HhaI GCGC 1 cut(s) 131
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HinfI GANTC 8 cut(s) 74, 221, 229, 467, 693, 1055, 1299, 1730
HpaII CCGG 1 cut(s) 1620
HphI GGTGA 5 cut(s) 485, 646, 1149, 1238, 1280
Hpy166II GTNNAC 5 cut(s) 401, 654, 991, 1137, 1343
Hpy188III TCNNGA 3 cut(s) 437, 479, 1784
Hpy8I GTNNAC 5 cut(s) 401, 654, 991, 1137, 1343
HpyAV CCTTC 7 cut(s) 8, 43, 386, 1030, 1685, 1702, 1772
HpyCH4IV ACGT 2 cut(s) 650, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 126, 1014, 1020, 1473, 1649
HpyF3I CTNAG 1 cut(s) 331
HpySE526I ACGT 2 cut(s) 650, 1194
HspAI GCGC 1 cut(s) 129
KpnI GGTACC 1 cut(s) 1549
Ksp22I TGATCA 3 cut(s) 445, 475, 1537
Kzo9I GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
LguI GCTCTTC 1 cut(s) 779
LmnI GCTCC 2 cut(s) 209, 967
Lsp1109I GCAGC 4 cut(s) 132, 551, 663, 1001
LweI GCATC 5 cut(s) 313, 420, 485, 946, 1010
MaeI CTAG 2 cut(s) 864, 1784
MaeII ACGT 2 cut(s) 650, 1194
MaeIII GTNAC 4 cut(s) 192, 281, 601, 1526
MalI GATC 8 cut(s) 447, 477, 529, 818, 946, 1156, 1444, 1539
MboI GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
MboII GAAGA 6 cut(s) 23, 79, 766, 1256, 1259, 1325
MfeI CAATTG 1 cut(s) 233
MflI RGATCY 3 cut(s) 527, 944, 1442
MhlI GDGCHC 3 cut(s) 269, 993, 1139
MlsI TGGCCA 1 cut(s) 1199
MluCI AATT 8 cut(s) 97, 233, 682, 1107, 1273, 1326, 1486, 1623
MluNI TGGCCA 1 cut(s) 1199
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 1201
Mox20I TGGCCA 1 cut(s) 1199
Mph1103I ATGCAT 1 cut(s) 328
MscI TGGCCA 1 cut(s) 1199
MseI TTAA 4 cut(s) 462, 1439, 1626, 1697
MslI CAYNNNNRTG 1 cut(s) 1029
Msp20I TGGCCA 1 cut(s) 1199
MspA1I CMGCKG 1 cut(s) 1589
MspI CCGG 1 cut(s) 1620
MunI CAATTG 1 cut(s) 233
Mva1269I GAATGC 4 cut(s) 47, 65, 556, 760
MvnI CGCG 1 cut(s) 457
MwoI GCNNNNNNNGC 5 cut(s) 126, 1014, 1020, 1473, 1649
NdeII GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
NlaIV GGNNCC 5 cut(s) 258, 491, 1453, 1547, 1566
NmuCI GTSAC 2 cut(s) 192, 1526
NsiI ATGCAT 1 cut(s) 328
NspI RCATGY 5 cut(s) 330, 370, 862, 1002, 1380
NspV TTCGAA 2 cut(s) 145, 307
PaeI GCATGC 2 cut(s) 330, 1002
PagI TCATGA 1 cut(s) 478
PaqCI CACCTGC 1 cut(s) 999
PciI ACATGT 3 cut(s) 366, 858, 1376
PciSI GCTCTTC 1 cut(s) 779
PctI GAATGC 4 cut(s) 47, 65, 556, 760
PfeI GAWTC 7 cut(s) 74, 221, 467, 693, 1055, 1299, 1730
PflFI GACNNNGTC 1 cut(s) 511
PkrI GCNGC 4 cut(s) 122, 541, 678, 1016
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PpuMI RGGWCCY 1 cut(s) 1759
PscI ACATGT 3 cut(s) 366, 858, 1376
PshAI GACNNNNGTC 1 cut(s) 720
PsiI TTATAA 1 cut(s) 630
Psp1406I AACGTT 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 1759
PspN4I GGNNCC 5 cut(s) 258, 491, 1453, 1547, 1566
PspPI GGNCC 2 cut(s) 1451, 1759
PspPPI RGGWCCY 1 cut(s) 1759
PstNI CAGNNNCTG 2 cut(s) 353, 1014
PsuI RGATCY 3 cut(s) 527, 944, 1442
PsyI GACNNNGTC 1 cut(s) 511
PvuII CAGCTG 1 cut(s) 1589
RsaI GTAC 6 cut(s) 346, 402, 823, 1073, 1463, 1547
RsaNI GTAC 6 cut(s) 345, 401, 822, 1072, 1462, 1546
RseI CAYNNNNRTG 1 cut(s) 1029
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 4 cut(s) 462, 1439, 1626, 1697
SatI GCNGC 4 cut(s) 121, 540, 677, 1015
Sau3AI GATC 8 cut(s) 445, 475, 527, 816, 944, 1154, 1442, 1537
Sau96I GGNCC 2 cut(s) 1451, 1759
SchI GAGTC 1 cut(s) 238
SduI GDGCHC 3 cut(s) 269, 993, 1139
SfaNI GCATC 5 cut(s) 313, 420, 485, 946, 1010
SfcI CTRYAG 2 cut(s) 1206, 1239
SfuI TTCGAA 2 cut(s) 145, 307
SinI GGWCC 1 cut(s) 1759
SmiMI CAYNNNNRTG 1 cut(s) 1029
SmlI CTYRAG 2 cut(s) 733, 1346
SmoI CTYRAG 2 cut(s) 733, 1346
SphI GCATGC 2 cut(s) 330, 1002
Sse9I AATT 8 cut(s) 97, 233, 682, 1107, 1273, 1326, 1486, 1623
SsiI CCGC 3 cut(s) 160, 901, 1017
SspMI CTAG 2 cut(s) 864, 1784
TaiI ACGT 2 cut(s) 653, 1197
TaqI TCGA 6 cut(s) 145, 227, 307, 436, 566, 714
TasI AATT 8 cut(s) 97, 233, 682, 1107, 1273, 1326, 1486, 1623
TatI WGTACW 2 cut(s) 344, 400
TfiI GAWTC 7 cut(s) 74, 221, 467, 693, 1055, 1299, 1730
Tru1I TTAA 4 cut(s) 462, 1439, 1626, 1697
Tru9I TTAA 4 cut(s) 462, 1439, 1626, 1697
TscAI CASTG 3 cut(s) 267, 1000, 1144
TseFI GTSAC 2 cut(s) 192, 1526
TseI GCWGC 4 cut(s) 120, 539, 676, 1014
Tsp45I GTSAC 2 cut(s) 192, 1526
TspDTI ATGAA 6 cut(s) 326, 405, 563, 755, 894, 1047
TspGWI ACGGA 1 cut(s) 839
TspRI CASTG 3 cut(s) 267, 1000, 1144
Tth111I GACNNNGTC 1 cut(s) 511
VneI GTGCAC 2 cut(s) 989, 1135
VpaK11BI GGWCC 1 cut(s) 1759
XapI RAATTY 4 cut(s) 97, 682, 1107, 1273
XbaI TCTAGA 1 cut(s) 1783
XceI RCATGY 5 cut(s) 330, 370, 862, 1002, 1380
XcmI CCANNNNNNNNNTGG 1 cut(s) 1593
XspI CTAG 2 cut(s) 864, 1784
Zsp2I ATGCAT 1 cut(s) 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.