Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007644

Physical Location & Seq

Genomic Coordinates Forward (+)

9811637 .. 9823805

Gene Structure 12169 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1134800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2232 bp

ATGAAGTTGTTGAGTGGAACAGACGGTGCAGGCGGTGGTGGTGGGGGGAAGACGGTGGTGGTGGGAGTGAAGTTAGACTCACACAGCAAGGAACTGCTGACGTGGGCACTCGTTAAAGTGGCGGAGACTGGTGACAATGTCGTTGCCATTCATGTTCTTGAGGACCACCTTCTCGCTACCTCTGATGGGACTTCGTCATTGCTCTCACTCGTGAAGACATTCGACTCTGTGCTCTCTGCCTATGAAGGTTTCTGCAACTTGAAGCAGGTTGATCTGAAGCTTAAGGTCTGTAGAGGTCATTCGGTCCGAAAGCTGCTCGTTCGGGAAGCAAAAGCATTTCATGCGGCTACTACCATTGTTGGAACATCTAAAACCCACCACAGAATTGGATCATCATCGACTTCCGTTGCCAAGTACTGTGCGAGAAAATTGTCTAAAAATTTCTCTGTTTTCGCTGTTGATAATGGCAAAATTGTGTTCAAGAGGGTCGGAACTGATAGGACTGCAGATCAATTTCAAGGTACTGGTCAGAACCGGAGCTATGTTCATACTAGCTTGGTTGGTGAGTCACTGAGCAAGAATGCAAAAGCAACTAATGATTCTGAACTAAATCAATGTCGACATTTCGTAAGAAAATCCTGTTCCAAGTGTGGAAAGAAAACCCTTCAGATAAATTGTGAGACTTGTGTTGGAGATCCAGTTTTGCTGGACAATTCTGGGACTAAATTGGCAGATGAGTTGCCAGCGGAAGGTGTTGAAGACAATTCATTGGCTTTGGTACCAATCCAAAGACTAGACCTCGCTTCAAACTCTTTCAAGCTTCAAGATTCACAGTGTTTGAAACCTGGTTGGTCGCTTATACGTCAGGCCTTTTTGCCAAAGCGGCAACACGTGGAGAAATCTGAGAAAAAGACATCTGTTTTTCGATGGGTCCTGAGACCATTGAGCTGGCATACTTCATCTGTAATCTACCCTGATCAGAAACAGAATAATTCTTGCCATGGTGAAGATTCTTCTTCCGTGCTGGATGGAATAAGTGGAGCAATTGTGCCGTTTGAGCCGGATGCAATCTACTCTCCTCTATTCCCTAACCATGGCAATGACCCCCTTCCTGAAGAATTTGTGGATTCATGTAAGAAATTCTCATCCAGTTGTAGATTGTTTAGCTACGAGGAGCTTTTGTCAGCAACATCCAACTTCATTCCTGAGAACATGGTTGGTAAAGGTGGCAGTAGTTATGTTTATAGAGGGTGTCTTTTAGATGGAAAGGAGTGGGCAGTGAAAATCCTGAAGCCATCTGGAGATATATTAAAAGAGTTTGTTCATGAGATTGAGATAATCAAATCTTTAAACCACAAGAACATAATCTCCCTGGTTGGATTTTGTTTCGAGGAGAACCATTTACTTTTGGTCTATGATTTTATTTCAAGAGGAAGCTTAGAAGAGAACCTCCATGGTAATAAGAAGGATGCAAATGCATTTGGTTGGAAGCAGAGATATAATGTGGCGATAGGTGTGGCGGAGGCACTTGATTATCTACACAATGGTTGTGAAGAACCTGTAATCCACAGGGATGTGAAGTCTTCCAATATCCTTCTTTCCGATGATTTCGAGCCGCAGCTCTCAGATTTTGGACTTGCTTGTTCGGCATCAGTGTCAACCGATATTAGCTGTACAGATGTGGCAGGAACATTTGGTTACTTGGCTCCAGAATACTTCATGCATGGCAAAGTGAGTGATAAAATTGATGTCTATGCATTTGGAGTCGTGGTCCTTGAGATTCTTTCTGGGAGAAAACCAATCTTCAGTAAGGACCCTAAGGGCCAGGAAAGTCTGGTGATGTGGGCAAAGCCAATTTTGAAGAGCGGGAACGTCGCCCAACTGCTAGATCCAAGCCTGGGTATTGCCTATGACCATGATCAAATTGAGAGAATGATTTTGGCTGCTAACCTTTGTATTAGACATGCACCTGGCCTTCGGCCTAATATCAGCGTAGTTTTGAAGCTTCTGCAAGGAGATGAAGAAGTGACAAGGTGGGCAAGACAAGTGAGTGTATCAGAAGAAGATGAAGCCGTGGATGGAGAAGCACCATCTTGTAACATTCAATCCCATCTTAACCTTGCACTGCGAGATTTGGAAGATGATTCCGTTTCTGTTAGCAGTGGCGAGCAAAGCATCGCTTTAGAGGATTACTTGCAAGGCAGATGGAGCCGCTCATCAAGCTTTAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0019899 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051020 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase Usp

Protein Analysis

744

Amino Acids

81.42

Weight (kDa)

6.11

Isoelectric Point (pI)

40.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Usp	PF00582	17 - 146	8.5e-09	Universal stress protein family
Pkinase	PF00069	403 - 608	6.9e-42	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	404 - 605	1.9e-37	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007644)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63940
fragaria_vesca	FvH4_1g24700 FvH4_1g24700 FvH4_1g24700 FvH4_1g24700 FvH4_1g24700
malus_domestica	MD15G1134800.v1.1
prunus_persica	Prupe.4G275600_v2.0.a1
pyrus_communis	pycom15g12160
rosa_chinensis	RchiOBHm_Chr2g0122201
rosa_laevigata	RLG00000018670
rosa_multiflora	Rmu_co8318615.1_g000001 Rmu_sc0003133.1_g000014 Rmu_sc0013150.1_g000002 Rmu_sc0025362.1_g000001
rosa_roxburghii	Rroxscaffold_2G00121770
rosa_rugosa	Rorug02G0237200 Rorug05G0310500
rosa_samantha	Rh2AG295700 Rh2BG304700 Rh2CG283100 Rh2DG319700
rosa_wichuraiana	Rw2G023550 Rw2G023780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	256
Acc65I	GGTACC	1 cut(s)	778
AccB1I	GGYRCC	1 cut(s)	778
AccBSI	CCGCTC	2 cut(s)	1866, 2214
AccI	GTMKAC	1 cut(s)	619
AciI	CCGC	9 cut(s)	33, 122, 344, 746, 883, 1520, 1616, 1866, 2212
AclWI	GGATC	3 cut(s)	397, 689, 1883
AcsI	RAATTY	3 cut(s)	439, 1118, 1139
AcuI	CTGAAG	5 cut(s)	296, 650, 1134, 1310, 1789
AcvI	CACGTG	1 cut(s)	892
AfaI	GTAC	4 cut(s)	416, 523, 780, 1675
AfiI	CCNNNNNNNGG	4 cut(s)	186, 749, 1094, 1898
AflII	CTTAAG	1 cut(s)	281
AflIII	ACRYGT	1 cut(s)	889
AjiI	CACGTC	1 cut(s)	102
AjnI	CCWGG	5 cut(s)	844, 1371, 1824, 1896, 1969
AloI	GAACNNNNNNTCC	4 cut(s)	1352, 1384, 1548, 1580
Alw21I	GWGCWC	1 cut(s)	234
Alw26I	GTCTC	3 cut(s)	119, 674, 931
AlwI	GGATC	3 cut(s)	397, 689, 1883
AoxI	GGCC	4 cut(s)	867, 1822, 1972, 1979
ApeKI	GCWGC	3 cut(s)	313, 1618, 1943
ApoI	RAATTY	3 cut(s)	439, 1118, 1139
Asp700I	GAANNNNTTC	1 cut(s)	218
Asp718I	GGTACC	1 cut(s)	778
AspS9I	GGNCC	6 cut(s)	163, 304, 931, 1771, 1813, 1822
AsuHPI	GGTGA	4 cut(s)	143, 575, 1016, 1849
AvaII	GGWCC	5 cut(s)	163, 304, 931, 1771, 1813
AxyI	CCTNAGG	1 cut(s)	1818
BaeGI	GKGCMC	1 cut(s)	109
BanI	GGYRCC	1 cut(s)	778
BauI	CACGAG	1 cut(s)	209
BbrPI	CACGTG	1 cut(s)	892
BbsI	GAAGAC	4 cut(s)	56, 221, 765, 1575
Bbv12I	GWGCWC	1 cut(s)	234
BbvI	GCAGC	3 cut(s)	300, 1630, 1930
BccI	CCATC	9 cut(s)	179, 921, 1022, 1256, 1303, 2072, 2098, 2118, 2199
BceAI	ACGGC	2 cut(s)	1036, 2057
BciT130I	CCWGG	5 cut(s)	846, 1373, 1826, 1898, 1971
BclI	TGATCA	2 cut(s)	976, 1918
BcoDI	GTCTC	3 cut(s)	119, 674, 931
BfaI	CTAG	4 cut(s)	552, 794, 1886, 2230
BfmI	CTRYAG	2 cut(s)	289, 504
BfrI	CTTAAG	1 cut(s)	281
BfuAI	ACCTGC	1 cut(s)	256
BglI	GCCNNNNNGGC	1 cut(s)	883
BisI	GCNGC	7 cut(s)	314, 345, 884, 1616, 1619, 1944, 2212
BlsI	GCNGC	7 cut(s)	315, 346, 885, 1617, 1620, 1945, 2213
BmcAI	AGTACT	1 cut(s)	416
Bme1390I	CCNGG	5 cut(s)	846, 1373, 1826, 1898, 1971
Bme18I	GGWCC	5 cut(s)	163, 304, 931, 1771, 1813
BmgBI	CACGTC	1 cut(s)	102
BmgT120I	GGNCC	6 cut(s)	163, 304, 931, 1771, 1813, 1822
BmiI	GGNNCC	5 cut(s)	780, 932, 1707, 1815, 2210
BmrFI	CCNGG	5 cut(s)	846, 1373, 1826, 1898, 1971
BmsI	GCATC	4 cut(s)	1054, 1459, 1658, 2184
BpiI	GAAGAC	4 cut(s)	56, 221, 765, 1575
BpmI	CTGGAG	2 cut(s)	1320, 1692
BpuEI	CTTGAG	2 cut(s)	179, 1796
BsaAI	YACGTR	1 cut(s)	892
BsaBI	GATNNNNATC	1 cut(s)	394
BsaI	GGTCTC	1 cut(s)	931
BsaJI	CCNNGG	6 cut(s)	1000, 1093, 1371, 1453, 1897, 2073
BsaWI	WCCGGW	1 cut(s)	534
Bsc4I	CCNNNNNNNGG	4 cut(s)	186, 749, 1094, 1898
Bse1I	ACTGG	4 cut(s)	133, 529, 698, 1149
Bse21I	CCTNAGG	1 cut(s)	1818
Bse3DI	GCAATG	2 cut(s)	197, 1105
Bse8I	GATNNNNATC	1 cut(s)	394
BseBI	CCWGG	5 cut(s)	846, 1373, 1826, 1898, 1971
BseDI	CCNNGG	6 cut(s)	1000, 1093, 1371, 1453, 1897, 2073
BseGI	GGATG	7 cut(s)	1033, 1069, 1145, 1190, 1474, 1579, 2083
BseJI	GATNNNNATC	1 cut(s)	394
BseLI	CCNNNNNNNGG	4 cut(s)	186, 749, 1094, 1898
BseMI	GCAATG	2 cut(s)	197, 1105
BseMII	CTCAG	5 cut(s)	563, 894, 926, 1197, 1638
BseNI	ACTGG	4 cut(s)	133, 529, 698, 1149
BseRI	GAGGAG	3 cut(s)	1068, 1187, 1406
BseSI	GKGCMC	1 cut(s)	109
BseXI	GCAGC	3 cut(s)	300, 1630, 1930
BsgI	GTGCAG	1 cut(s)	48
BshFI	GGCC	4 cut(s)	869, 1824, 1974, 1981
BshNI	GGYRCC	1 cut(s)	778
BsiHKAI	GWGCWC	1 cut(s)	234
BsiSI	CCGG	2 cut(s)	535, 1061
BslFI	GGGAC	2 cut(s)	202, 733
BslI	CCNNNNNNNGG	4 cut(s)	186, 749, 1094, 1898
BsmAI	GTCTC	3 cut(s)	119, 674, 931
BsmFI	GGGAC	2 cut(s)	202, 733
BsmI	GAATGC	1 cut(s)	586
BsnI	GGCC	4 cut(s)	869, 1824, 1974, 1981
Bso31I	GGTCTC	1 cut(s)	931
Bsp1286I	GDGCHC	2 cut(s)	109, 234
Bsp1407I	TGTACA	1 cut(s)	1673
Bsp143I	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
Bsp19I	CCATGG	3 cut(s)	1000, 1093, 1453
BspACI	CCGC	9 cut(s)	33, 122, 344, 746, 883, 1520, 1616, 1866, 2212
BspANI	GGCC	4 cut(s)	869, 1824, 1974, 1981
BspCNI	CTCAG	5 cut(s)	564, 895, 927, 1198, 1637
BspHI	TCATGA	1 cut(s)	1324
BspLI	GGNNCC	5 cut(s)	780, 932, 1707, 1815, 2210
BspMAI	CTGCAG	1 cut(s)	508
BspMI	ACCTGC	1 cut(s)	256
BspPI	GGATC	3 cut(s)	397, 689, 1883
BspQI	GCTCTTC	1 cut(s)	1856
BspT107I	GGYRCC	1 cut(s)	778
BspTI	CTTAAG	1 cut(s)	281
BspTNI	GGTCTC	1 cut(s)	931
BsrBI	CCGCTC	2 cut(s)	1866, 2214
BsrDI	GCAATG	2 cut(s)	197, 1105
BsrGI	TGTACA	1 cut(s)	1673
BsrI	ACTGG	4 cut(s)	133, 529, 698, 1149
BssECI	CCNNGG	6 cut(s)	1000, 1093, 1371, 1453, 1897, 2073
BssMI	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
BssSI	CACGAG	1 cut(s)	209
BssT1I	CCWWGG	3 cut(s)	1000, 1093, 1453
Bst2BI	CACGAG	1 cut(s)	209
Bst2UI	CCWGG	5 cut(s)	846, 1373, 1826, 1898, 1971
Bst4CI	ACNGT	4 cut(s)	26, 55, 419, 834
Bst6I	CTCTTC	2 cut(s)	1437, 1856
BstAFI	CTTAAG	1 cut(s)	281
BstAPI	GCANNNNNTGC	1 cut(s)	341
BstAUI	TGTACA	1 cut(s)	1673
BstBAI	YACGTR	1 cut(s)	892
BstC8I	GCNNGC	4 cut(s)	31, 744, 950, 2168
BstDEI	CTNAG	7 cut(s)	572, 903, 935, 1206, 1438, 1624, 1818
BstDSI	CCRYGG	4 cut(s)	1000, 1093, 1453, 2073
BstF5I	GGATG	7 cut(s)	1033, 1069, 1145, 1190, 1474, 1579, 2083
BstKTI	GATC	7 cut(s)	274, 392, 511, 697, 979, 1891, 1921
BstMAI	GTCTC	3 cut(s)	119, 674, 931
BstMBI	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
BstMWI	GCNNNNNNNGC	7 cut(s)	341, 883, 1057, 1646, 2172, 2208, 2220
BstNI	CCWGG	5 cut(s)	846, 1373, 1826, 1898, 1971
BstNSI	RCATGY	1 cut(s)	1967
BstSCI	CCNGG	5 cut(s)	844, 1371, 1824, 1896, 1969
BstSFI	CTRYAG	2 cut(s)	289, 504
BstSLI	GKGCMC	1 cut(s)	109
BstV1I	GCAGC	3 cut(s)	300, 1630, 1930
BstV2I	GAAGAC	4 cut(s)	56, 221, 765, 1575
BstX2I	RGATCY	2 cut(s)	694, 1888
BstXI	CCANNNNNNTGG	2 cut(s)	386, 948
BstYI	RGATCY	2 cut(s)	694, 1888
Bsu36I	CCTNAGG	1 cut(s)	1818
BsuRI	GGCC	4 cut(s)	869, 1824, 1974, 1981
BtgI	CCRYGG	4 cut(s)	1000, 1093, 1453, 2073
BtgZI	GCGATG	1 cut(s)	2161
BtrI	CACGTC	1 cut(s)	102
BtsCI	GGATG	7 cut(s)	1033, 1069, 1145, 1190, 1474, 1579, 2083
BtsI	GCAGTG	3 cut(s)	1284, 2123, 2167
BtsIMutI	CAGTG	6 cut(s)	569, 839, 1284, 1659, 2123, 2167
BveI	ACCTGC	1 cut(s)	256
Cac8I	GCNNGC	4 cut(s)	31, 744, 950, 2168
CciI	TCATGA	1 cut(s)	1324
Cfr13I	GGNCC	6 cut(s)	163, 304, 931, 1771, 1813, 1822
CpoI	CGGWCCG	1 cut(s)	304
CsiI	ACCWGGT	1 cut(s)	844
Csp6I	GTAC	4 cut(s)	415, 522, 779, 1674
CspI	CGGWCCG	1 cut(s)	304
CviQI	GTAC	4 cut(s)	415, 522, 779, 1674
DdeI	CTNAG	7 cut(s)	572, 903, 935, 1206, 1438, 1624, 1818
DpnI	GATC	7 cut(s)	273, 391, 510, 696, 978, 1890, 1920
DpnII	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
DraI	TTTAAA	1 cut(s)	1350
Eam1104I	CTCTTC	2 cut(s)	1437, 1856
EarI	CTCTTC	2 cut(s)	1437, 1856
EciI	GGCGGA	2 cut(s)	137, 1535
Eco130I	CCWWGG	3 cut(s)	1000, 1093, 1453
Eco147I	AGGCCT	1 cut(s)	869
Eco31I	GGTCTC	1 cut(s)	931
Eco47I	GGWCC	5 cut(s)	163, 304, 931, 1771, 1813
Eco57I	CTGAAG	5 cut(s)	296, 650, 1134, 1310, 1789
Eco72I	CACGTG	1 cut(s)	892
Eco81I	CCTNAGG	1 cut(s)	1818
EcoO109I	RGGNCCY	2 cut(s)	931, 1813
EcoRII	CCWGG	5 cut(s)	844, 1371, 1824, 1896, 1969
EcoT14I	CCWWGG	3 cut(s)	1000, 1093, 1453
EcoT22I	ATGCAT	3 cut(s)	1480, 1725, 1759
ErhI	CCWWGG	3 cut(s)	1000, 1093, 1453
FalI	AAGNNNNNCTT	2 cut(s)	2164, 2196
FaqI	GGGAC	2 cut(s)	202, 733
FauI	CCCGC	1 cut(s)	1859
FbaI	TGATCA	2 cut(s)	976, 1918
FblI	GTMKAC	1 cut(s)	619
Fnu4HI	GCNGC	7 cut(s)	314, 345, 884, 1616, 1619, 1944, 2212
FokI	GGATG	7 cut(s)	1040, 1076, 1132, 1177, 1481, 1586, 2090
Fsp4HI	GCNGC	7 cut(s)	314, 345, 884, 1616, 1619, 1944, 2212
FspBI	CTAG	4 cut(s)	552, 794, 1886, 2230
GluI	GCNGC	7 cut(s)	314, 345, 884, 1616, 1619, 1944, 2212
GsuI	CTGGAG	2 cut(s)	1320, 1692
HaeIII	GGCC	4 cut(s)	869, 1824, 1974, 1981
HapII	CCGG	2 cut(s)	535, 1061
HincII	GTYRAC	2 cut(s)	620, 1659
HindII	GTYRAC	2 cut(s)	620, 1659
HindIII	AAGCTT	5 cut(s)	278, 818, 1435, 2003, 2221
HpaII	CCGG	2 cut(s)	535, 1061
HphI	GGTGA	4 cut(s)	143, 575, 1016, 1849
Hpy166II	GTNNAC	2 cut(s)	620, 1659
Hpy8I	GTNNAC	2 cut(s)	620, 1659
Hpy99I	CGWCG	1 cut(s)	1877
HpyAV	CCTTC	8 cut(s)	179, 239, 674, 743, 1118, 1459, 1604, 1985
HpyCH4III	ACNGT	4 cut(s)	26, 55, 419, 834
HpyCH4IV	ACGT	4 cut(s)	101, 862, 891, 1872
HpyF10VI	GCNNNNNNNGC	7 cut(s)	341, 883, 1057, 1646, 2172, 2208, 2220
HpyF3I	CTNAG	7 cut(s)	572, 903, 935, 1206, 1438, 1624, 1818
HpySE526I	ACGT	4 cut(s)	101, 862, 891, 1872
KpnI	GGTACC	1 cut(s)	782
Ksp22I	TGATCA	2 cut(s)	976, 1918
Kzo9I	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
LguI	GCTCTTC	1 cut(s)	1856
LmnI	GCTCC	5 cut(s)	537, 1040, 1174, 1711, 2208
Lsp1109I	GCAGC	3 cut(s)	300, 1630, 1930
LweI	GCATC	4 cut(s)	1054, 1459, 1658, 2184
MabI	ACCWGGT	1 cut(s)	844
MaeI	CTAG	4 cut(s)	552, 794, 1886, 2230
MaeII	ACGT	4 cut(s)	101, 862, 891, 1872
MaeIII	GTNAC	5 cut(s)	131, 567, 1697, 2026, 2096
MalI	GATC	7 cut(s)	273, 391, 510, 696, 978, 1890, 1920
MbiI	CCGCTC	2 cut(s)	1866, 2214
MboI	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
MfeI	CAATTG	1 cut(s)	1044
MflI	RGATCY	2 cut(s)	694, 1888
MhlI	GDGCHC	2 cut(s)	109, 234
MlyI	GAGTC	4 cut(s)	71, 218, 575, 1773
MmeI	TCCRAC	6 cut(s)	340, 469, 670, 1218, 1357, 1466
Mph1103I	ATGCAT	3 cut(s)	1480, 1725, 1759
MroXI	GAANNNNTTC	1 cut(s)	218
MseI	TTAA	6 cut(s)	114, 282, 1310, 1349, 2115, 2226
MslI	CAYNNNNRTG	2 cut(s)	1098, 1572
MspA1I	CMGCKG	1 cut(s)	746
MspCI	CTTAAG	1 cut(s)	281
MspI	CCGG	2 cut(s)	535, 1061
MspR9I	CCNGG	5 cut(s)	846, 1373, 1826, 1898, 1971
MunI	CAATTG	1 cut(s)	1044
Mva1269I	GAATGC	1 cut(s)	586
MvaI	CCWGG	5 cut(s)	846, 1373, 1826, 1898, 1971
MwoI	GCNNNNNNNGC	7 cut(s)	341, 883, 1057, 1646, 2172, 2208, 2220
NcoI	CCATGG	3 cut(s)	1000, 1093, 1453
NdeII	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
NlaIV	GGNNCC	5 cut(s)	780, 932, 1707, 1815, 2210
NmuCI	GTSAC	3 cut(s)	131, 567, 2026
NsiI	ATGCAT	3 cut(s)	1480, 1725, 1759
NspI	RCATGY	1 cut(s)	1967
PagI	TCATGA	1 cut(s)	1324
PceI	AGGCCT	1 cut(s)	869
PciSI	GCTCTTC	1 cut(s)	1856
PctI	GAATGC	1 cut(s)	586
PdmI	GAANNNNTTC	1 cut(s)	218
PfeI	GAWTC	6 cut(s)	599, 827, 1010, 1127, 1780, 2144
PflFI	GACNNNGTC	2 cut(s)	137, 193
PkrI	GCNGC	7 cut(s)	315, 346, 885, 1617, 1620, 1945, 2213
PleI	GAGTC	4 cut(s)	71, 218, 574, 1772
PmaCI	CACGTG	1 cut(s)	892
PmlI	CACGTG	1 cut(s)	892
PpsI	GAGTC	4 cut(s)	71, 218, 574, 1772
Ppu21I	YACGTR	1 cut(s)	892
PpuMI	RGGWCCY	2 cut(s)	931, 1813
Psp5II	RGGWCCY	2 cut(s)	931, 1813
Psp6I	CCWGG	5 cut(s)	844, 1371, 1824, 1896, 1969
PspCI	CACGTG	1 cut(s)	892
PspGI	CCWGG	5 cut(s)	844, 1371, 1824, 1896, 1969
PspN4I	GGNNCC	5 cut(s)	780, 932, 1707, 1815, 2210
PspPI	GGNCC	6 cut(s)	163, 304, 931, 1771, 1813, 1822
PspPPI	RGGWCCY	2 cut(s)	931, 1813
PstI	CTGCAG	1 cut(s)	508
PsuI	RGATCY	2 cut(s)	694, 1888
PsyI	GACNNNGTC	2 cut(s)	137, 193
RsaI	GTAC	4 cut(s)	416, 523, 780, 1675
RsaNI	GTAC	4 cut(s)	415, 522, 779, 1674
RseI	CAYNNNNRTG	2 cut(s)	1098, 1572
Rsr2I	CGGWCCG	1 cut(s)	304
RsrII	CGGWCCG	1 cut(s)	304
SalI	GTCGAC	1 cut(s)	618
SapI	GCTCTTC	1 cut(s)	1856
SaqAI	TTAA	6 cut(s)	114, 282, 1310, 1349, 2115, 2226
SatI	GCNGC	7 cut(s)	314, 345, 884, 1616, 1619, 1944, 2212
Sau3AI	GATC	7 cut(s)	271, 389, 508, 694, 976, 1888, 1918
Sau96I	GGNCC	6 cut(s)	163, 304, 931, 1771, 1813, 1822
ScaI	AGTACT	1 cut(s)	416
SchI	GAGTC	4 cut(s)	71, 218, 575, 1773
ScrFI	CCNGG	5 cut(s)	846, 1373, 1826, 1898, 1971
SduI	GDGCHC	2 cut(s)	109, 234
SexAI	ACCWGGT	1 cut(s)	844
SfaNI	GCATC	4 cut(s)	1054, 1459, 1658, 2184
SfcI	CTRYAG	2 cut(s)	289, 504
SinI	GGWCC	5 cut(s)	163, 304, 931, 1771, 1813
SmiMI	CAYNNNNRTG	2 cut(s)	1098, 1572
SmlI	CTYRAG	3 cut(s)	158, 281, 1775
SmoI	CTYRAG	3 cut(s)	158, 281, 1775
SseBI	AGGCCT	1 cut(s)	869
SsiI	CCGC	9 cut(s)	33, 122, 344, 746, 883, 1520, 1616, 1866, 2212
SspMI	CTAG	4 cut(s)	552, 794, 1886, 2230
StuI	AGGCCT	1 cut(s)	869
StyD4I	CCNGG	5 cut(s)	844, 1371, 1824, 1896, 1969
StyI	CCWWGG	3 cut(s)	1000, 1093, 1453
TaaI	ACNGT	4 cut(s)	26, 55, 419, 834
TaiI	ACGT	4 cut(s)	104, 865, 894, 1875
TaqI	TCGA	6 cut(s)	222, 398, 619, 925, 1389, 1611
TaqII	GACCGA	1 cut(s)	292
TatI	WGTACW	2 cut(s)	414, 1673
TauI	GCSGC	4 cut(s)	347, 886, 1618, 2214
TfiI	GAWTC	6 cut(s)	599, 827, 1010, 1127, 1780, 2144
Tru1I	TTAA	6 cut(s)	114, 282, 1310, 1349, 2115, 2226
Tru9I	TTAA	6 cut(s)	114, 282, 1310, 1349, 2115, 2226
TscAI	CASTG	6 cut(s)	576, 839, 1284, 1659, 2130, 2167
TseFI	GTSAC	3 cut(s)	131, 567, 2026
TseI	GCWGC	3 cut(s)	313, 1618, 1943
Tsp45I	GTSAC	3 cut(s)	131, 567, 2026
TspGWI	ACGGA	3 cut(s)	394, 1009, 2137
TspRI	CASTG	6 cut(s)	576, 839, 1284, 1659, 2130, 2167
Tth111I	GACNNNGTC	2 cut(s)	137, 193
Vha464I	CTTAAG	1 cut(s)	281
VpaK11BI	GGWCC	5 cut(s)	163, 304, 931, 1771, 1813
XapI	RAATTY	3 cut(s)	439, 1118, 1139
XceI	RCATGY	1 cut(s)	1967
XcmI	CCANNNNNNNNNTGG	1 cut(s)	383
XmiI	GTMKAC	1 cut(s)	619
XmnI	GAANNNNTTC	1 cut(s)	218
XspI	CTAG	4 cut(s)	552, 794, 1886, 2230
ZrmI	AGTACT	1 cut(s)	416
Zsp2I	ATGCAT	3 cut(s)	1480, 1725, 1759

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.