Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014394

Physical Location & Seq

Genomic Coordinates Reverse (-)

10752431 .. 10754432

Gene Structure 2002 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1145200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1122 bp

ATGATGAACATGAACTCATCAATCTCAGACTCCTTCAAAACAAACCCTATCAACCTCTCCCACATTATCCCACTCGATTTCGAATCCGTTCATGCCCTACCCGATTCCCACGTCTGGACTCATAGGAAGGAGGTTGAGATTCCAACACCTTATAATACTCCGTCTTTAGTCTGCGTACCCGTCATTGACCTATTCGATTCGAACGCCGCCTCTCTCATACGCCGTGCATGCGAGAAATGGGGTGTCTTCCAAGTCACCAACCACGGCATTCCCATGCACCTCATGAAAGAAGCCGAGTTCCAAACCCGTCGCTTATTCGCTCTCCCGAGAGACCAAAAGCTCCGGGCCCTCCGATCACCCGAGGGAGTAACCGGGTACGGGTTGGCCCGCATTTCGAAGAACTTCCCGAAGCTGATGTGGTCAGAGGGCTTCACCATCATGGGGTCTCCGGCGGAACATGCTCTTCAACTTTGGCCACGTCACCATGCCAACTTCTGCGATGCAATGGAAGAGTACCAAAAGGAGATGAAGGGCCTAGCCCTAAAATTAATTGGGCTCATGCTTGGATCATTGGGCCTGAACTACGAAGATGTGAAGTGGCTTAAGCCCAAAAATAGCAGCAGGCCACAGACCGTGCTTCAACTGAACTCCTACCCGGTCTGCCCAGACCCGACCCGGGCAATGGGTCTCGCTCCTCACACGGACTCCTCATTGCTCACTTTGCTAAACCAAAGCAGCATAAGCGGCCTCCAAGTCCTTGTGGACGGGATCCGGTGGGTGCCGGCGCACCCGATCCCCGGCGCCCTTGTAGTCAATGTCGGTGATTTAATGCACATACTGTCGAACGGACAATTCAAAAGTGCGGCGCATCGTGCGGTTGTCAACGAAGTACACCATCGCATCTCGATGGCGTACTTCTATGGCCCGCCGATGGACGTGAAGATATCGCCGTTGATTGACCACAATCATCCACTCTATCAGCCGGTAACATGGAAGGAGTACCTCGATTACAAAGCAACGCATTTCGACAAGGCGCTTGAAGTAATCCGGACAGATGCCCAGAAGCTGTACCAGCGTACATCTGATGATTTTGCCTTCGGCCAATGGCCCAATTGTACATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

374

Amino Acids

42.17

Weight (kDa)

7.76

Isoelectric Point (pI)

39.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	59 - 159	1.5e-20	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	212 - 309	8.7e-31	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014394)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21690
fragaria_vesca	FvH4_1g04200
malus_domestica	MD15G1145200.v1.1
prunus_persica	Prupe.7G235400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0089681 RchiOBHm_Chr2g0089691
rosa_laevigata	RLG00000016051
rosa_multiflora	Rmu_sc0000665.1_g000022
rosa_roxburghii	Rroxscaffold_2G00151670
rosa_rugosa	Rorug02G0002600
rosa_samantha	Rh2AG048000 Rh2BG046600 Rh2BG046700 Rh2CG048600 Rh2CG048800 Rh2DG048000
rosa_wichuraiana	Rw2G004250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	153
AccB1I	GGYRCC	2 cut(s)	778, 800
AccIII	TCCGGA	1 cut(s)	1047
AciI	CCGC	7 cut(s)	207, 388, 452, 744, 863, 875, 926
AclWI	GGATC	4 cut(s)	574, 763, 776, 787
AcoI	YGGCCR	2 cut(s)	473, 1099
AcyI	GRCGYC	1 cut(s)	801
AfaI	GTAC	9 cut(s)	177, 377, 515, 891, 914, 1001, 1070, 1078, 1117
AfiI	CCNNNNNNNGG	7 cut(s)	114, 378, 441, 676, 682, 797, 931
AflII	CTTAAG	1 cut(s)	602
AgsI	TTSAA	5 cut(s)	37, 467, 641, 856, 1040
AjiI	CACGTC	3 cut(s)	112, 479, 937
AluBI	AGCT	3 cut(s)	340, 412, 1066
AluI	AGCT	3 cut(s)	340, 412, 1066
Alw26I	GTCTC	3 cut(s)	324, 450, 692
AlwI	GGATC	4 cut(s)	574, 763, 776, 787
AlwNI	CAGNNNCTG	1 cut(s)	1066
Ama87I	CYCGRG	3 cut(s)	325, 359, 675
Aor13HI	TCCGGA	1 cut(s)	1047
ApaI	GGGCCC	1 cut(s)	349
ApeKI	GCWGC	2 cut(s)	618, 735
AseI	ATTAAT	1 cut(s)	548
Asp700I	GAANNNNTTC	2 cut(s)	87, 401
AspLEI	GCGC	4 cut(s)	787, 803, 868, 1036
AspS9I	GGNCC	7 cut(s)	345, 346, 385, 532, 574, 923, 1107
AsuC2I	CCSGG	6 cut(s)	344, 373, 656, 676, 677, 798
AsuHPI	GGTGA	5 cut(s)	247, 348, 424, 473, 833
AsuII	TTCGAA	3 cut(s)	81, 200, 395
AvaI	CYCGRG	3 cut(s)	325, 359, 675
BaeGI	GKGCMC	1 cut(s)	349
BalI	TGGCCA	1 cut(s)	475
BamHI	GGATCC	1 cut(s)	768
BanI	GGYRCC	2 cut(s)	778, 800
BanII	GRGCYC	2 cut(s)	349, 558
BbsI	GAAGAC	1 cut(s)	238
BbvI	GCAGC	2 cut(s)	630, 747
BccI	CCATC	4 cut(s)	443, 901, 903, 925
BceAI	ACGGC	3 cut(s)	207, 280, 934
BcnI	CCSGG	6 cut(s)	344, 373, 656, 676, 677, 798
BcoDI	GTCTC	3 cut(s)	324, 450, 692
BfaI	CTAG	1 cut(s)	536
BfoI	RGCGCY	2 cut(s)	804, 1037
BfrI	CTTAAG	1 cut(s)	602
BisI	GCNGC	5 cut(s)	207, 619, 736, 745, 864
BlsI	GCNGC	5 cut(s)	208, 620, 737, 746, 865
Bme1390I	CCNGG	6 cut(s)	344, 373, 656, 676, 677, 798
BmeT110I	CYCGRG	3 cut(s)	325, 359, 675
BmgBI	CACGTC	3 cut(s)	112, 479, 937
BmgT120I	GGNCC	7 cut(s)	345, 346, 385, 532, 574, 923, 1107
BmiI	GGNNCC	4 cut(s)	347, 770, 780, 802
BmrFI	CCNGG	6 cut(s)	344, 373, 656, 676, 677, 798
BmsI	GCATC	4 cut(s)	490, 877, 909, 1045
BpiI	GAAGAC	1 cut(s)	238
Bpu14I	TTCGAA	3 cut(s)	81, 200, 395
BpuMI	CCSGG	6 cut(s)	344, 373, 656, 676, 677, 798
BsaHI	GRCGYC	1 cut(s)	801
BsaI	GGTCTC	3 cut(s)	324, 450, 692
BsaJI	CCNNGG	4 cut(s)	262, 360, 675, 796
BsaWI	WCCGGW	2 cut(s)	771, 1047
BsaXI	ACNNNNNCTCC	2 cut(s)	689, 719
Bsc4I	CCNNNNNNNGG	7 cut(s)	114, 378, 441, 676, 682, 797, 931
Bse118I	RCCGGY	2 cut(s)	781, 982
Bse3DI	GCAATG	3 cut(s)	510, 687, 710
BseAI	TCCGGA	1 cut(s)	1047
BseDI	CCNNGG	4 cut(s)	262, 360, 675, 796
BseGI	GGATG	1 cut(s)	967
BseLI	CCNNNNNNNGG	7 cut(s)	114, 378, 441, 676, 682, 797, 931
BseMI	GCAATG	3 cut(s)	510, 687, 710
BseMII	CTCAG	1 cut(s)	39
BseRI	GAGGAG	2 cut(s)	684, 697
BseSI	GKGCMC	1 cut(s)	349
BseXI	GCAGC	2 cut(s)	630, 747
BshNI	GGYRCC	2 cut(s)	778, 800
BsiHKCI	CYCGRG	3 cut(s)	325, 359, 675
BslI	CCNNNNNNNGG	7 cut(s)	114, 378, 441, 676, 682, 797, 931
BsmAI	GTCTC	3 cut(s)	324, 450, 692
BsmI	GAATGC	1 cut(s)	267
Bso31I	GGTCTC	3 cut(s)	324, 450, 692
BsoBI	CYCGRG	3 cut(s)	325, 359, 675
Bsp119I	TTCGAA	3 cut(s)	81, 200, 395
Bsp120I	GGGCCC	1 cut(s)	345
Bsp1286I	GDGCHC	2 cut(s)	349, 558
Bsp13I	TCCGGA	1 cut(s)	1047
Bsp1407I	TGTACA	1 cut(s)	1115
Bsp143I	GATC	4 cut(s)	353, 566, 768, 792
BspACI	CCGC	7 cut(s)	207, 388, 452, 744, 863, 875, 926
BspCNI	CTCAG	1 cut(s)	38
BspEI	TCCGGA	1 cut(s)	1047
BspHI	TCATGA	1 cut(s)	282
BspLI	GGNNCC	4 cut(s)	347, 770, 780, 802
BspPI	GGATC	4 cut(s)	574, 763, 776, 787
BspQI	GCTCTTC	1 cut(s)	468
BspT104I	TTCGAA	3 cut(s)	81, 200, 395
BspT107I	GGYRCC	2 cut(s)	778, 800
BspTI	CTTAAG	1 cut(s)	602
BspTNI	GGTCTC	3 cut(s)	324, 450, 692
BsrDI	GCAATG	3 cut(s)	510, 687, 710
BsrFI	RCCGGY	2 cut(s)	781, 982
BsrGI	TGTACA	1 cut(s)	1115
BssAI	RCCGGY	2 cut(s)	781, 982
BssECI	CCNNGG	4 cut(s)	262, 360, 675, 796
BssMI	GATC	4 cut(s)	353, 566, 768, 792
BssNI	GRCGYC	1 cut(s)	801
Bst4CI	ACNGT	2 cut(s)	634, 840
Bst6I	CTCTTC	2 cut(s)	468, 504
BstACI	GRCGYC	1 cut(s)	801
BstAFI	CTTAAG	1 cut(s)	602
BstAUI	TGTACA	1 cut(s)	1115
BstBI	TTCGAA	3 cut(s)	81, 200, 395
BstC8I	GCNNGC	5 cut(s)	229, 388, 623, 783, 926
BstDEI	CTNAG	1 cut(s)	25
BstDSI	CCRYGG	1 cut(s)	262
BstF5I	GGATG	1 cut(s)	967
BstH2I	RGCGCY	2 cut(s)	804, 1037
BstHHI	GCGC	4 cut(s)	787, 803, 868, 1036
BstKTI	GATC	4 cut(s)	356, 569, 771, 795
BstMAI	GTCTC	3 cut(s)	324, 450, 692
BstMBI	GATC	4 cut(s)	353, 566, 768, 792
BstMWI	GCNNNNNNNGC	7 cut(s)	228, 458, 721, 741, 744, 872, 1072
BstNSI	RCATGY	2 cut(s)	231, 461
BstSCI	CCNGG	6 cut(s)	342, 371, 654, 674, 675, 796
BstSLI	GKGCMC	1 cut(s)	349
BstV1I	GCAGC	2 cut(s)	630, 747
BstV2I	GAAGAC	1 cut(s)	238
BstX2I	RGATCY	1 cut(s)	768
BstYI	RGATCY	1 cut(s)	768
BtgI	CCRYGG	1 cut(s)	262
BtgZI	GCGATG	2 cut(s)	513, 881
BtrI	CACGTC	3 cut(s)	112, 479, 937
BtsCI	GGATG	1 cut(s)	967
Cac8I	GCNNGC	5 cut(s)	229, 388, 623, 783, 926
CaiI	CAGNNNCTG	1 cut(s)	1066
CciI	TCATGA	1 cut(s)	282
CfoI	GCGC	4 cut(s)	787, 803, 868, 1036
Cfr10I	RCCGGY	2 cut(s)	781, 982
Cfr13I	GGNCC	7 cut(s)	345, 346, 385, 532, 574, 923, 1107
Cfr9I	CCCGGG	1 cut(s)	675
Csp6I	GTAC	9 cut(s)	176, 376, 514, 890, 913, 1000, 1069, 1077, 1116
CviQI	GTAC	9 cut(s)	176, 376, 514, 890, 913, 1000, 1069, 1077, 1116
DdeI	CTNAG	1 cut(s)	25
DinI	GGCGCC	1 cut(s)	802
DpnI	GATC	4 cut(s)	355, 568, 770, 794
DpnII	GATC	4 cut(s)	353, 566, 768, 792
EaeI	YGGCCR	2 cut(s)	473, 1099
Eam1104I	CTCTTC	2 cut(s)	468, 504
EarI	CTCTTC	2 cut(s)	468, 504
EciI	GGCGGA	1 cut(s)	467
Eco24I	GRGCYC	2 cut(s)	349, 558
Eco31I	GGTCTC	3 cut(s)	324, 450, 692
Eco32I	GATATC	1 cut(s)	945
Eco88I	CYCGRG	3 cut(s)	325, 359, 675
EcoO109I	RGGNCCY	2 cut(s)	346, 532
EcoRV	GATATC	1 cut(s)	945
EcoT38I	GRGCYC	2 cut(s)	349, 558
EgeI	GGCGCC	1 cut(s)	802
EheI	GGCGCC	1 cut(s)	802
FauI	CCCGC	2 cut(s)	395, 933
Fnu4HI	GCNGC	5 cut(s)	207, 619, 736, 745, 864
FokI	GGATG	1 cut(s)	954
FriOI	GRGCYC	2 cut(s)	349, 558
Fsp4HI	GCNGC	5 cut(s)	207, 619, 736, 745, 864
FspBI	CTAG	1 cut(s)	536
GlaI	GCGC	4 cut(s)	786, 802, 867, 1035
GluI	GCNGC	5 cut(s)	207, 619, 736, 745, 864
HaeII	RGCGCY	2 cut(s)	804, 1037
HhaI	GCGC	4 cut(s)	787, 803, 868, 1036
Hin1I	GRCGYC	1 cut(s)	801
Hin6I	GCGC	4 cut(s)	785, 801, 866, 1034
HinP1I	GCGC	4 cut(s)	785, 801, 866, 1034
HincII	GTYRAC	1 cut(s)	883
HindII	GTYRAC	1 cut(s)	883
HinfI	GANTC	7 cut(s)	29, 83, 104, 118, 139, 197, 704
HphI	GGTGA	5 cut(s)	247, 348, 424, 473, 833
Hpy166II	GTNNAC	3 cut(s)	763, 883, 892
Hpy188I	TCNGA	4 cut(s)	28, 353, 424, 1084
Hpy188III	TCNNGA	6 cut(s)	115, 283, 325, 406, 904, 1048
Hpy8I	GTNNAC	3 cut(s)	763, 883, 892
Hpy99I	CGWCG	1 cut(s)	312
HpyAV	CCTTC	5 cut(s)	43, 121, 523, 988, 1105
HpyCH4III	ACNGT	2 cut(s)	634, 840
HpyCH4IV	ACGT	3 cut(s)	111, 478, 936
HpyCH4V	TGCA	4 cut(s)	227, 277, 503, 832
HpyF10VI	GCNNNNNNNGC	7 cut(s)	228, 458, 721, 741, 744, 872, 1072
HpyF3I	CTNAG	1 cut(s)	25
HpySE526I	ACGT	3 cut(s)	111, 478, 936
Hsp92I	GRCGYC	1 cut(s)	801
HspAI	GCGC	4 cut(s)	785, 801, 866, 1034
KasI	GGCGCC	1 cut(s)	800
Kpn2I	TCCGGA	1 cut(s)	1047
KroI	GCCGGC	1 cut(s)	781
KroNI	GCCGGC	1 cut(s)	783
Kzo9I	GATC	4 cut(s)	353, 566, 768, 792
LguI	GCTCTTC	1 cut(s)	468
LmnI	GCTCC	2 cut(s)	345, 697
Lsp1109I	GCAGC	2 cut(s)	630, 747
LweI	GCATC	4 cut(s)	490, 877, 909, 1045
MaeI	CTAG	1 cut(s)	536
MaeII	ACGT	3 cut(s)	111, 478, 936
MaeIII	GTNAC	4 cut(s)	253, 367, 479, 985
MalI	GATC	4 cut(s)	355, 568, 770, 794
MboI	GATC	4 cut(s)	353, 566, 768, 792
MboII	GAAGA	6 cut(s)	238, 409, 455, 521, 599, 952
MfeI	CAATTG	1 cut(s)	1111
MflI	RGATCY	1 cut(s)	768
MhlI	GDGCHC	2 cut(s)	349, 558
MlsI	TGGCCA	1 cut(s)	475
MluCI	AATT	4 cut(s)	545, 549, 851, 1111
MluNI	TGGCCA	1 cut(s)	475
Mly113I	GGCGCC	1 cut(s)	801
MlyI	GAGTC	3 cut(s)	23, 112, 698
MmeI	TCCRAC	1 cut(s)	167
Mox20I	TGGCCA	1 cut(s)	475
MroI	TCCGGA	1 cut(s)	1047
MroNI	GCCGGC	1 cut(s)	781
MroXI	GAANNNNTTC	2 cut(s)	87, 401
MscI	TGGCCA	1 cut(s)	475
MseI	TTAA	3 cut(s)	548, 603, 827
MslI	CAYNNNNRTG	3 cut(s)	272, 437, 905
Msp20I	TGGCCA	1 cut(s)	475
MspCI	CTTAAG	1 cut(s)	602
MspR9I	CCNGG	6 cut(s)	344, 373, 656, 676, 677, 798
MunI	CAATTG	1 cut(s)	1111
Mva1269I	GAATGC	1 cut(s)	267
MwoI	GCNNNNNNNGC	7 cut(s)	228, 458, 721, 741, 744, 872, 1072
NaeI	GCCGGC	1 cut(s)	783
NarI	GGCGCC	1 cut(s)	801
NciI	CCSGG	6 cut(s)	344, 373, 656, 676, 677, 798
NdeII	GATC	4 cut(s)	353, 566, 768, 792
NgoMIV	GCCGGC	1 cut(s)	781
NlaIV	GGNNCC	4 cut(s)	347, 770, 780, 802
NmeAIII	GCCGAG	1 cut(s)	319
NmuCI	GTSAC	2 cut(s)	253, 479
NspI	RCATGY	2 cut(s)	231, 461
NspV	TTCGAA	3 cut(s)	81, 200, 395
PaeI	GCATGC	1 cut(s)	231
PagI	TCATGA	1 cut(s)	282
PciSI	GCTCTTC	1 cut(s)	468
PctI	GAATGC	1 cut(s)	267
PdiI	GCCGGC	1 cut(s)	783
PdmI	GAANNNNTTC	2 cut(s)	87, 401
PfeI	GAWTC	4 cut(s)	83, 104, 139, 197
PkrI	GCNGC	5 cut(s)	208, 620, 737, 746, 865
PleI	GAGTC	3 cut(s)	23, 112, 698
PluTI	GGCGCC	1 cut(s)	804
PpsI	GAGTC	3 cut(s)	23, 112, 698
PshBI	ATTAAT	1 cut(s)	548
PsiI	TTATAA	1 cut(s)	153
PspN4I	GGNNCC	4 cut(s)	347, 770, 780, 802
PspOMI	GGGCCC	1 cut(s)	345
PspPI	GGNCC	7 cut(s)	345, 346, 385, 532, 574, 923, 1107
PstNI	CAGNNNCTG	1 cut(s)	1066
PsuI	RGATCY	1 cut(s)	768
RsaI	GTAC	9 cut(s)	177, 377, 515, 891, 914, 1001, 1070, 1078, 1117
RsaNI	GTAC	9 cut(s)	176, 376, 514, 890, 913, 1000, 1069, 1077, 1116
RseI	CAYNNNNRTG	3 cut(s)	272, 437, 905
SapI	GCTCTTC	1 cut(s)	468
SaqAI	TTAA	3 cut(s)	548, 603, 827
SatI	GCNGC	5 cut(s)	207, 619, 736, 745, 864
Sau3AI	GATC	4 cut(s)	353, 566, 768, 792
Sau96I	GGNCC	7 cut(s)	345, 346, 385, 532, 574, 923, 1107
SchI	GAGTC	3 cut(s)	23, 112, 698
ScrFI	CCNGG	6 cut(s)	344, 373, 656, 676, 677, 798
SduI	GDGCHC	2 cut(s)	349, 558
SfaNI	GCATC	4 cut(s)	490, 877, 909, 1045
SfoI	GGCGCC	1 cut(s)	802
SfuI	TTCGAA	3 cut(s)	81, 200, 395
SmaI	CCCGGG	1 cut(s)	677
SmiMI	CAYNNNNRTG	3 cut(s)	272, 437, 905
SmlI	CTYRAG	1 cut(s)	602
SmoI	CTYRAG	1 cut(s)	602
SphI	GCATGC	1 cut(s)	231
Sse9I	AATT	4 cut(s)	545, 549, 851, 1111
SsiI	CCGC	7 cut(s)	207, 388, 452, 744, 863, 875, 926
SspDI	GGCGCC	1 cut(s)	800
SspMI	CTAG	1 cut(s)	536
StyD4I	CCNGG	6 cut(s)	342, 371, 654, 674, 675, 796
TaaI	ACNGT	2 cut(s)	634, 840
TaiI	ACGT	3 cut(s)	114, 481, 939
TaqI	TCGA	9 cut(s)	75, 81, 195, 200, 395, 842, 905, 1005, 1026
TasI	AATT	4 cut(s)	545, 549, 851, 1111
TatI	WGTACW	2 cut(s)	889, 1115
TauI	GCSGC	3 cut(s)	209, 747, 866
TfiI	GAWTC	4 cut(s)	83, 104, 139, 197
Tru1I	TTAA	3 cut(s)	548, 603, 827
Tru9I	TTAA	3 cut(s)	548, 603, 827
TseFI	GTSAC	2 cut(s)	253, 479
TseI	GCWGC	2 cut(s)	618, 735
Tsp45I	GTSAC	2 cut(s)	253, 479
TspDTI	ATGAA	5 cut(s)	20, 26, 80, 299, 542
TspGWI	ACGGA	4 cut(s)	76, 150, 716, 861
TspMI	CCCGGG	1 cut(s)	675
Vha464I	CTTAAG	1 cut(s)	602
VspI	ATTAAT	1 cut(s)	548
XceI	RCATGY	2 cut(s)	231, 461
XmaI	CCCGGG	1 cut(s)	675
XmnI	GAANNNNTTC	2 cut(s)	87, 401
XspI	CTAG	1 cut(s)	536

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.