Rh2BG046600

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014394
Physical Location & Seq
Reverse (-)
3313054 .. 3313644
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG046600.1

Sequence Viewer

Length: 591 bp
ATGGAAGAGTACCAAAAGAAGATGAAGGGCCTATGTGGAAAATTGATAAGGCTGATGCTTGGATCACTGGGCTTGGACCATGAAGATGTTAAATGGCTGAACCGGCCCAAAAAGAGTTTTCCACAGGTCTTGCAACTGAACTCGTATCCGGTTTGTCCGGACCCGACCCGAGCCATGGGGTTGGCTCCTCACACAGACTCCTCAATTTTCACAGTGCTAAACCAAAGCAACGTAAGTGGCCTGCAAGTCTTTGAGGATGGGATTGGTTGGGTGCCGGTTCACCCGATCCCCGGTGCACTTGTAGTCAATGTAGGTGATTTGATGCACATACTATCCAACGGCCGGTTCAAGGCTGCAGTGCATCGTGCGGTTGTCAATGAGCTATATCACCGCATCTCCATCGCCTACTTCTACAGCCCACCGTACGACGTGAAGATATCACCGCCGATGAAGTTGATCGACCAAGATCATCCTCCCCTATATCGACCGGTAACTTGGAAGGAATACCTTGGTATCAAAGGAACACATTTCAACAGGGCACTTGATCTAATCAGGAATGACACGGACCAAGCACCATACATGCATGAATAG

Protein Analysis

196

Amino Acids

22.24

Weight (kDa)

8.47

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 42 - 140 2e-33 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21690
fragaria_vesca FvH4_1g04200
malus_domestica MD15G1145200.v1.1
prunus_persica Prupe.7G235400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089681 RchiOBHm_Chr2g0089691
rosa_laevigata RLG00000016051
rosa_multiflora Rmu_sc0000665.1_g000022
rosa_roxburghii Rroxscaffold_2G00151670
rosa_rugosa Rorug02G0002600
rosa_samantha Rh2AG048000 Rh2BG046600 Rh2BG046700 Rh2CG048600 Rh2CG048800 Rh2DG048000
rosa_wichuraiana Rw2G004250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 271
AccIII TCCGGA 1 cut(s) 157
AciI CCGC 3 cut(s) 368, 391, 443
AclWI GGATC 2 cut(s) 70, 280
AcoI YGGCCR 1 cut(s) 340
AfaI GTAC 2 cut(s) 11, 425
AfiI CCNNNNNNNGG 4 cut(s) 175, 290, 342, 349
AgeI ACCGGT 1 cut(s) 487
AgsI TTSAA 2 cut(s) 349, 532
AjiI CACGTC 1 cut(s) 430
AjuI GAANNNNNNNTTGG 2 cut(s) 329, 361
AluBI AGCT 1 cut(s) 382
AluI AGCT 1 cut(s) 382
Alw21I GWGCWC 1 cut(s) 298
Alw44I GTGCAC 1 cut(s) 294
AlwI GGATC 2 cut(s) 70, 280
Ama87I CYCGRG 1 cut(s) 168
Aor13HI TCCGGA 1 cut(s) 157
AoxI GGCC 4 cut(s) 28, 104, 238, 340
ApaLI GTGCAC 1 cut(s) 294
ApeKI GCWGC 1 cut(s) 353
AsiGI ACCGGT 1 cut(s) 487
AspS9I GGNCC 5 cut(s) 28, 76, 105, 160, 565
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 4 cut(s) 272, 326, 380, 432
AvaI CYCGRG 1 cut(s) 168
AvaII GGWCC 3 cut(s) 76, 160, 565
BaeGI GKGCMC 2 cut(s) 298, 541
BaeI ACNNNNGTAYC 2 cut(s) 496, 529
BanI GGYRCC 1 cut(s) 271
Bbv12I GWGCWC 1 cut(s) 298
BbvI GCAGC 1 cut(s) 340
BccI CCATC 2 cut(s) 251, 407
BceAI ACGGC 1 cut(s) 355
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciVI GTATCC 1 cut(s) 156
BcnI CCSGG 1 cut(s) 291
BfmI CTRYAG 2 cut(s) 354, 412
BfuI GTATCC 1 cut(s) 156
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 3 cut(s) 76, 160, 565
BmeT110I CYCGRG 1 cut(s) 168
BmgBI CACGTC 1 cut(s) 430
BmgT120I GGNCC 5 cut(s) 28, 76, 105, 160, 565
BmiI GGNNCC 3 cut(s) 162, 186, 273
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 77
BmsI GCATC 4 cut(s) 45, 312, 370, 402
BmuI ACTGGG 1 cut(s) 77
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 3 cut(s) 174, 289, 508
BsaWI WCCGGW 3 cut(s) 148, 157, 487
BsaXI ACNNNNNCTCC 4 cut(s) 182, 212, 380, 410
Bsc4I CCNNNNNNNGG 4 cut(s) 175, 290, 342, 349
Bse118I RCCGGY 4 cut(s) 102, 274, 342, 487
Bse1I ACTGG 1 cut(s) 72
BseAI TCCGGA 1 cut(s) 157
BseDI CCNNGG 3 cut(s) 174, 289, 508
BseGI GGATG 2 cut(s) 262, 469
BseLI CCNNNNNNNGG 4 cut(s) 175, 290, 342, 349
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 177, 190
BseSI GKGCMC 2 cut(s) 298, 541
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 1 cut(s) 340
Bsh1285I CGRYCG 2 cut(s) 343, 488
BshFI GGCC 4 cut(s) 30, 106, 240, 342
BshNI GGYRCC 1 cut(s) 271
BshTI ACCGGT 1 cut(s) 487
BsiEI CGRYCG 2 cut(s) 343, 488
BsiHKAI GWGCWC 1 cut(s) 298
BsiHKCI CYCGRG 1 cut(s) 168
BsiSI CCGG 7 cut(s) 103, 149, 158, 275, 291, 343, 488
BsiWI CGTACG 1 cut(s) 423
BslI CCNNNNNNNGG 4 cut(s) 175, 290, 342, 349
BsnI GGCC 4 cut(s) 30, 106, 240, 342
BsoBI CYCGRG 1 cut(s) 168
Bsp1286I GDGCHC 2 cut(s) 298, 541
Bsp13I TCCGGA 1 cut(s) 157
Bsp143I GATC 5 cut(s) 62, 285, 456, 466, 544
Bsp19I CCATGG 1 cut(s) 174
BspACI CCGC 3 cut(s) 368, 391, 443
BspANI GGCC 4 cut(s) 30, 106, 240, 342
BspEI TCCGGA 1 cut(s) 157
BspLI GGNNCC 3 cut(s) 162, 186, 273
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 2 cut(s) 70, 280
BspT107I GGYRCC 1 cut(s) 271
BsrFI RCCGGY 4 cut(s) 102, 274, 342, 487
BsrI ACTGG 1 cut(s) 72
BssAI RCCGGY 4 cut(s) 102, 274, 342, 487
BssECI CCNNGG 3 cut(s) 174, 289, 508
BssMI GATC 5 cut(s) 62, 285, 456, 466, 544
BssT1I CCWWGG 2 cut(s) 174, 508
Bst4CI ACNGT 2 cut(s) 214, 423
BstC8I GCNNGC 1 cut(s) 242
BstDSI CCRYGG 1 cut(s) 174
BstF5I GGATG 2 cut(s) 262, 469
BstKTI GATC 5 cut(s) 65, 288, 459, 469, 547
BstMBI GATC 5 cut(s) 62, 285, 456, 466, 544
BstMCI CGRYCG 2 cut(s) 343, 488
BstMWI GCNNNNNNNGC 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 583
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 2 cut(s) 354, 412
BstSLI GKGCMC 2 cut(s) 298, 541
BstV1I GCAGC 1 cut(s) 340
BstXI CCANNNNNNTGG 1 cut(s) 181
BstZI CGGCCG 1 cut(s) 340
BsuI GTATCC 1 cut(s) 156
BsuRI GGCC 4 cut(s) 30, 106, 240, 342
BtgI CCRYGG 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 385
BtrI CACGTC 1 cut(s) 430
BtsCI GGATG 2 cut(s) 262, 469
BtsI GCAGTG 1 cut(s) 363
BtsIMutI CAGTG 3 cut(s) 65, 219, 363
Cac8I GCNNGC 1 cut(s) 242
Cfr10I RCCGGY 4 cut(s) 102, 274, 342, 487
Cfr13I GGNCC 5 cut(s) 28, 76, 105, 160, 565
Csp6I GTAC 2 cut(s) 10, 424
CspAI ACCGGT 1 cut(s) 487
CspCI CAANNNNNGTGG 4 cut(s) 111, 146, 217, 252
CviAII CATG 4 cut(s) 80, 175, 580, 584
CviQI GTAC 2 cut(s) 10, 424
DpnI GATC 5 cut(s) 64, 287, 458, 468, 546
DpnII GATC 5 cut(s) 62, 285, 456, 466, 544
EaeI YGGCCR 1 cut(s) 340
EagI CGGCCG 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 340
Eco130I CCWWGG 2 cut(s) 174, 508
Eco32I GATATC 1 cut(s) 438
Eco47I GGWCC 3 cut(s) 76, 160, 565
Eco52I CGGCCG 1 cut(s) 340
Eco88I CYCGRG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 28
EcoRV GATATC 1 cut(s) 438
EcoT14I CCWWGG 2 cut(s) 174, 508
EcoT22I ATGCAT 1 cut(s) 585
ErhI CCWWGG 2 cut(s) 174, 508
FaeI CATG 4 cut(s) 83, 178, 583, 587
FaiI YATR 9 cut(s) 34, 81, 176, 329, 385, 481, 577, 581, 585
FatI CATG 4 cut(s) 79, 174, 579, 583
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 2 cut(s) 269, 456
Fsp4HI GCNGC 1 cut(s) 354
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 4 cut(s) 30, 106, 240, 342
HapII CCGG 7 cut(s) 103, 149, 158, 275, 291, 343, 488
Hin1II CATG 4 cut(s) 83, 178, 583, 587
HinfI GANTC 1 cut(s) 197
HpaII CCGG 7 cut(s) 103, 149, 158, 275, 291, 343, 488
HphI GGTGA 4 cut(s) 272, 326, 380, 432
Hpy166II GTNNAC 2 cut(s) 280, 296
Hpy188III TCNNGA 2 cut(s) 158, 553
Hpy8I GTNNAC 2 cut(s) 280, 296
Hpy99I CGWCG 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 19, 493
HpyCH4III ACNGT 2 cut(s) 214, 423
HpyCH4IV ACGT 2 cut(s) 231, 429
HpyCH4V TGCA 7 cut(s) 133, 244, 296, 325, 356, 361, 583
HpyF10VI GCNNNNNNNGC 1 cut(s) 103
HpySE526I ACGT 2 cut(s) 231, 429
Hsp92II CATG 4 cut(s) 83, 178, 583, 587
Kpn2I TCCGGA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 62, 285, 456, 466, 544
LmnI GCTCC 1 cut(s) 190
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 4 cut(s) 45, 312, 370, 402
MaeII ACGT 2 cut(s) 231, 429
MaeIII GTNAC 1 cut(s) 490
MalI GATC 5 cut(s) 64, 287, 458, 468, 546
MboI GATC 5 cut(s) 62, 285, 456, 466, 544
MboII GAAGA 4 cut(s) 17, 31, 95, 445
MhlI GDGCHC 2 cut(s) 298, 541
MluCI AATT 2 cut(s) 41, 204
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 4 cut(s) 198, 211, 247, 483
Mph1103I ATGCAT 1 cut(s) 585
MroI TCCGGA 1 cut(s) 157
MseI TTAA 1 cut(s) 90
MslI CAYNNNNRTG 1 cut(s) 84
MspI CCGG 7 cut(s) 103, 149, 158, 275, 291, 343, 488
MspR9I CCNGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 103
NciI CCSGG 1 cut(s) 291
NcoI CCATGG 1 cut(s) 174
NdeII GATC 5 cut(s) 62, 285, 456, 466, 544
NlaIII CATG 4 cut(s) 83, 178, 583, 587
NlaIV GGNNCC 3 cut(s) 162, 186, 273
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 1 cut(s) 583
Pfl23II CGTACG 1 cut(s) 423
PinAI ACCGGT 1 cut(s) 487
PkrI GCNGC 1 cut(s) 355
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
PspLI CGTACG 1 cut(s) 423
PspN4I GGNNCC 3 cut(s) 162, 186, 273
PspPI GGNCC 5 cut(s) 28, 76, 105, 160, 565
PstI CTGCAG 1 cut(s) 358
RsaI GTAC 2 cut(s) 11, 425
RsaNI GTAC 2 cut(s) 10, 424
RseI CAYNNNNRTG 1 cut(s) 84
SaqAI TTAA 1 cut(s) 90
SatI GCNGC 1 cut(s) 354
Sau3AI GATC 5 cut(s) 62, 285, 456, 466, 544
Sau96I GGNCC 5 cut(s) 28, 76, 105, 160, 565
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 1 cut(s) 291
SduI GDGCHC 2 cut(s) 298, 541
SetI ASST 6 cut(s) 129, 234, 316, 384, 432, 510
SfaNI GCATC 4 cut(s) 45, 312, 370, 402
SfcI CTRYAG 2 cut(s) 354, 412
SinI GGWCC 3 cut(s) 76, 160, 565
SmiMI CAYNNNNRTG 1 cut(s) 84
Sse9I AATT 2 cut(s) 41, 204
SsiI CCGC 3 cut(s) 368, 391, 443
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 2 cut(s) 174, 508
TaaI ACNGT 2 cut(s) 214, 423
TaiI ACGT 2 cut(s) 234, 432
TaqI TCGA 2 cut(s) 459, 484
TasI AATT 2 cut(s) 41, 204
Tru1I TTAA 1 cut(s) 90
Tru9I TTAA 1 cut(s) 90
TscAI CASTG 3 cut(s) 72, 219, 363
TseI GCWGC 1 cut(s) 353
TspDTI ATGAA 3 cut(s) 38, 96, 464
TspGWI ACGGA 1 cut(s) 578
TspRI CASTG 3 cut(s) 72, 219, 363
VneI GTGCAC 1 cut(s) 294
VpaK11BI GGWCC 3 cut(s) 76, 160, 565
XceI RCATGY 1 cut(s) 583
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.