Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0014394

Physical Location & Seq

Genomic Coordinates Forward (+)

88866206 .. 88867396

Gene Structure 1191 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00151670.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1095 bp

ATGAGTTCAACTGCTACCCGAAACCTCTCCCATATCGTCCCACTCGACTTCAACTCCATCCACACATTACCCGACTCCCACACTTGGGACTCCTCGACGGACCACCACCCCTTCACAGAACTCCTACCCATCATAGACCTACTCGACCCGAATGCCGCCTCGCTCATACAGCTTGCCTCTGAGCAATGGGGTGTCTTCCAAGTCACCAACCACGGCATTCCAATGAACTTAATCCAAGAAGCGGAGCTACAAGCCCGTGGATTGTTTGAGCTGCCTAGGGACCAAAAACTCCAGGCACTTCGATCACCGGAGAGCTTAACCGGTTATGGGCGAGCCCGCATTTCCGCCAACTTCTCAAAGCTGATGTGGTCGGAGGGTTTCACGATGATCGGGTCTCCGCTTGAACATGCGGCTCAACTCTGGCCAGATGACCACTCGATATTAGTCATGTTACTATGTGTGTATGACCATTTTGACCCTGAAGATGCCTGCTCATGCAGTAATGTAATGGAGGAGTACCAAAAGAAGATGAAGGGCCTATGTGGAAAATTGATAAGGCTGATGCTTGGCTCATTGGGCTTGGACCATGAAGATGTTAAATGGCTGAACCGGCCCAAAAAGAGTGGCAGAAATTTTCCACAGGTCTTGCAACTGAACTCGTATCCGGTTTGTCCGGACCCGACCCGAGCCATGGGGTTGGCTCCTCACACAGACTCCTCAATTTTCACGGTGCTAAACCAAAGCAACATAAGTGGCCTGCAAGTCTTTGAGGATGGGATTGGTTGGGTGCCGGTTCACCCGATCCCCGGTGCACTTGTAGTCAATGTAGGTGATTTGATGCACATACTATCCAACGGCCGGTTCAAGGCTGCAGTGCATCGTGCGGTTGTCAATGAGCTATATCACCGCATCTCCATCGCCTACTTCTACAGCCCACCGTACGACGTGAAGATATCGCCGCCGATGAAGTTGATCGACCAAGATCATCCTCCCCTATATCGACCGGTAACTTGGAAGGAATACCTTGGTATCAAGGGAACACATTTCAACAGGGCACTTGATCTAATCAGGAATGACACGGACCAAGCACCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko00904 ko01110 map00904 map01110

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

364

Amino Acids

40.9

Weight (kDa)

5.99

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	42 - 143	4.7e-18	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	214 - 312	1.7e-32	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014394)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21690
fragaria_vesca	FvH4_1g04200
malus_domestica	MD15G1145200.v1.1
prunus_persica	Prupe.7G235400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0089681 RchiOBHm_Chr2g0089691
rosa_laevigata	RLG00000016051
rosa_multiflora	Rmu_sc0000665.1_g000022
rosa_roxburghii	Rroxscaffold_2G00151670
rosa_rugosa	Rorug02G0002600
rosa_samantha	Rh2AG048000 Rh2BG046600 Rh2BG046700 Rh2CG048600 Rh2CG048800 Rh2DG048000
rosa_wichuraiana	Rw2G004250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	787
AccIII	TCCGGA	1 cut(s)	673
AciI	CCGC	9 cut(s)	156, 242, 337, 345, 398, 410, 884, 907, 959
AclWI	GGATC	1 cut(s)	796
AcoI	YGGCCR	2 cut(s)	422, 856
AcsI	RAATTY	1 cut(s)	631
AcuI	CTGAAG	1 cut(s)	501
AfaI	GTAC	2 cut(s)	518, 941
AfiI	CCNNNNNNNGG	8 cut(s)	84, 85, 241, 327, 691, 806, 858, 865
AgeI	ACCGGT	2 cut(s)	320, 1003
AgsI	TTSAA	5 cut(s)	9, 52, 404, 865, 1048
AjiI	CACGTC	1 cut(s)	946
AjnI	CCWGG	1 cut(s)	291
AjuI	GAANNNNNNNTTGG	2 cut(s)	845, 877
AluBI	AGCT	6 cut(s)	172, 247, 271, 315, 361, 898
AluI	AGCT	6 cut(s)	172, 247, 271, 315, 361, 898
Alw21I	GWGCWC	1 cut(s)	814
Alw26I	GTCTC	1 cut(s)	399
Alw44I	GTGCAC	1 cut(s)	810
AlwI	GGATC	1 cut(s)	796
Ama87I	CYCGRG	1 cut(s)	684
Aor13HI	TCCGGA	1 cut(s)	673
AoxI	GGCC	5 cut(s)	422, 535, 611, 754, 856
ApaLI	GTGCAC	1 cut(s)	810
ApeKI	GCWGC	2 cut(s)	271, 869
ApoI	RAATTY	1 cut(s)	631
AsiGI	ACCGGT	2 cut(s)	320, 1003
AspA2I	CCTAGG	1 cut(s)	275
AspS9I	GGNCC	7 cut(s)	100, 280, 535, 583, 612, 676, 1081
AsuC2I	CCSGG	1 cut(s)	807
AsuHPI	GGTGA	5 cut(s)	196, 297, 788, 842, 896
AvaI	CYCGRG	1 cut(s)	684
AvaII	GGWCC	5 cut(s)	100, 280, 583, 676, 1081
AvrII	CCTAGG	1 cut(s)	275
BaeGI	GKGCMC	2 cut(s)	814, 1057
BaeI	ACNNNNGTAYC	2 cut(s)	1012, 1045
BalI	TGGCCA	1 cut(s)	424
BanI	GGYRCC	1 cut(s)	787
BanII	GRGCYC	1 cut(s)	337
BarI	GAAGNNNNNNTAC	2 cut(s)	231, 263
BbsI	GAAGAC	1 cut(s)	187
Bbv12I	GWGCWC	1 cut(s)	814
BbvI	GCAGC	2 cut(s)	258, 856
BccI	CCATC	4 cut(s)	65, 137, 767, 923
BceAI	ACGGC	2 cut(s)	229, 871
BcgI	CGANNNNNNTGC	4 cut(s)	134, 168, 898, 932
BciT130I	CCWGG	1 cut(s)	293
BciVI	GTATCC	1 cut(s)	672
BcnI	CCSGG	1 cut(s)	807
BcoDI	GTCTC	1 cut(s)	399
BfaI	CTAG	1 cut(s)	276
BfmI	CTRYAG	2 cut(s)	870, 928
BfuI	GTATCC	1 cut(s)	672
BisI	GCNGC	5 cut(s)	156, 272, 411, 870, 959
BlnI	CCTAGG	1 cut(s)	275
BlsI	GCNGC	5 cut(s)	157, 273, 412, 871, 960
Bme1390I	CCNGG	2 cut(s)	293, 807
Bme18I	GGWCC	5 cut(s)	100, 280, 583, 676, 1081
BmeT110I	CYCGRG	1 cut(s)	684
BmgBI	CACGTC	1 cut(s)	946
BmgT120I	GGNCC	7 cut(s)	100, 280, 535, 583, 612, 676, 1081
BmiI	GGNNCC	4 cut(s)	281, 678, 702, 789
BmrFI	CCNGG	2 cut(s)	293, 807
BmsI	GCATC	5 cut(s)	475, 552, 828, 886, 918
BpiI	GAAGAC	1 cut(s)	187
BpmI	CTGGAG	1 cut(s)	275
BpuMI	CCSGG	1 cut(s)	807
BsaI	GGTCTC	1 cut(s)	399
BsaJI	CCNNGG	6 cut(s)	211, 256, 275, 690, 805, 1024
BsaWI	WCCGGW	5 cut(s)	307, 320, 664, 673, 1003
BsaXI	ACNNNNNCTCC	6 cut(s)	38, 68, 698, 728, 896, 926
Bsc4I	CCNNNNNNNGG	8 cut(s)	84, 85, 241, 327, 691, 806, 858, 865
Bse118I	RCCGGY	5 cut(s)	320, 609, 790, 858, 1003
Bse3DI	GCAATG	1 cut(s)	191
BseAI	TCCGGA	1 cut(s)	673
BseBI	CCWGG	1 cut(s)	293
BseDI	CCNNGG	6 cut(s)	211, 256, 275, 690, 805, 1024
BseGI	GGATG	3 cut(s)	57, 778, 985
BseLI	CCNNNNNNNGG	8 cut(s)	84, 85, 241, 327, 691, 806, 858, 865
BseMI	GCAATG	1 cut(s)	191
BseMII	CTCAG	1 cut(s)	171
BseRI	GAGGAG	4 cut(s)	82, 527, 693, 706
BseSI	GKGCMC	2 cut(s)	814, 1057
BseX3I	CGGCCG	1 cut(s)	856
BseXI	GCAGC	2 cut(s)	258, 856
Bsh1285I	CGRYCG	2 cut(s)	859, 1004
BshFI	GGCC	5 cut(s)	424, 537, 613, 756, 858
BshNI	GGYRCC	1 cut(s)	787
BshTI	ACCGGT	2 cut(s)	320, 1003
BsiEI	CGRYCG	2 cut(s)	859, 1004
BsiHKAI	GWGCWC	1 cut(s)	814
BsiHKCI	CYCGRG	1 cut(s)	684
BsiSI	CCGG	9 cut(s)	308, 321, 610, 665, 674, 791, 807, 859, 1004
BsiWI	CGTACG	1 cut(s)	939
BslFI	GGGAC	3 cut(s)	23, 101, 293
BslI	CCNNNNNNNGG	8 cut(s)	84, 85, 241, 327, 691, 806, 858, 865
BsmAI	GTCTC	1 cut(s)	399
BsmFI	GGGAC	3 cut(s)	23, 101, 293
BsmI	GAATGC	2 cut(s)	157, 216
BsnI	GGCC	5 cut(s)	424, 537, 613, 756, 858
Bso31I	GGTCTC	1 cut(s)	399
BsoBI	CYCGRG	1 cut(s)	684
Bsp1286I	GDGCHC	3 cut(s)	337, 814, 1057
Bsp13I	TCCGGA	1 cut(s)	673
Bsp143I	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
Bsp19I	CCATGG	1 cut(s)	690
BspACI	CCGC	9 cut(s)	156, 242, 337, 345, 398, 410, 884, 907, 959
BspANI	GGCC	5 cut(s)	424, 537, 613, 756, 858
BspCNI	CTCAG	1 cut(s)	172
BspEI	TCCGGA	1 cut(s)	673
BspLI	GGNNCC	4 cut(s)	281, 678, 702, 789
BspMAI	CTGCAG	1 cut(s)	874
BspPI	GGATC	1 cut(s)	796
BspT107I	GGYRCC	1 cut(s)	787
BspTNI	GGTCTC	1 cut(s)	399
BsrDI	GCAATG	1 cut(s)	191
BsrFI	RCCGGY	5 cut(s)	320, 609, 790, 858, 1003
BssAI	RCCGGY	5 cut(s)	320, 609, 790, 858, 1003
BssECI	CCNNGG	6 cut(s)	211, 256, 275, 690, 805, 1024
BssMI	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
BssT1I	CCWWGG	3 cut(s)	275, 690, 1024
Bst2UI	CCWGG	1 cut(s)	293
Bst4CI	ACNGT	3 cut(s)	730, 939, 1092
BstC8I	GCNNGC	5 cut(s)	174, 333, 337, 490, 758
BstDEI	CTNAG	1 cut(s)	180
BstDSI	CCRYGG	3 cut(s)	211, 256, 690
BstF5I	GGATG	3 cut(s)	57, 778, 985
BstKTI	GATC	6 cut(s)	305, 390, 804, 975, 985, 1063
BstMAI	GTCTC	1 cut(s)	399
BstMBI	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
BstMCI	CGRYCG	2 cut(s)	859, 1004
BstMWI	GCNNNNNNNGC	3 cut(s)	169, 576, 610
BstNI	CCWGG	1 cut(s)	293
BstNSI	RCATGY	1 cut(s)	410
BstSCI	CCNGG	2 cut(s)	291, 805
BstSFI	CTRYAG	2 cut(s)	870, 928
BstSLI	GKGCMC	2 cut(s)	814, 1057
BstV1I	GCAGC	2 cut(s)	258, 856
BstV2I	GAAGAC	1 cut(s)	187
BstXI	CCANNNNNNTGG	1 cut(s)	697
BstZI	CGGCCG	1 cut(s)	856
BsuI	GTATCC	1 cut(s)	672
BsuRI	GGCC	5 cut(s)	424, 537, 613, 756, 858
BtgI	CCRYGG	3 cut(s)	211, 256, 690
BtgZI	GCGATG	1 cut(s)	901
BtrI	CACGTC	1 cut(s)	946
BtsCI	GGATG	3 cut(s)	57, 778, 985
BtsI	GCAGTG	1 cut(s)	879
BtsIMutI	CAGTG	1 cut(s)	879
Cac8I	GCNNGC	5 cut(s)	174, 333, 337, 490, 758
Cfr10I	RCCGGY	5 cut(s)	320, 609, 790, 858, 1003
Cfr13I	GGNCC	7 cut(s)	100, 280, 535, 583, 612, 676, 1081
Csp6I	GTAC	2 cut(s)	517, 940
CspAI	ACCGGT	2 cut(s)	320, 1003
CspCI	CAANNNNNGTGG	6 cut(s)	604, 627, 639, 662, 733, 768
CviAII	CATG	5 cut(s)	407, 448, 495, 587, 691
CviQI	GTAC	2 cut(s)	517, 940
DdeI	CTNAG	1 cut(s)	180
DpnI	GATC	6 cut(s)	304, 389, 803, 974, 984, 1062
DpnII	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
EaeI	YGGCCR	2 cut(s)	422, 856
EagI	CGGCCG	1 cut(s)	856
EciI	GGCGGA	1 cut(s)	334
EclXI	CGGCCG	1 cut(s)	856
Eco130I	CCWWGG	3 cut(s)	275, 690, 1024
Eco24I	GRGCYC	1 cut(s)	337
Eco31I	GGTCTC	1 cut(s)	399
Eco32I	GATATC	1 cut(s)	954
Eco47I	GGWCC	5 cut(s)	100, 280, 583, 676, 1081
Eco52I	CGGCCG	1 cut(s)	856
Eco57I	CTGAAG	1 cut(s)	501
Eco88I	CYCGRG	1 cut(s)	684
EcoO109I	RGGNCCY	1 cut(s)	535
EcoRII	CCWGG	1 cut(s)	291
EcoRV	GATATC	1 cut(s)	954
EcoT14I	CCWWGG	3 cut(s)	275, 690, 1024
EcoT38I	GRGCYC	1 cut(s)	337
ErhI	CCWWGG	3 cut(s)	275, 690, 1024
FaeI	CATG	5 cut(s)	410, 451, 498, 590, 694
FaqI	GGGAC	3 cut(s)	23, 101, 293
FatI	CATG	5 cut(s)	406, 447, 494, 586, 690
FauI	CCCGC	1 cut(s)	344
Fnu4HI	GCNGC	5 cut(s)	156, 272, 411, 870, 959
FokI	GGATG	3 cut(s)	44, 785, 972
FriOI	GRGCYC	1 cut(s)	337
Fsp4HI	GCNGC	5 cut(s)	156, 272, 411, 870, 959
FspBI	CTAG	1 cut(s)	276
GluI	GCNGC	5 cut(s)	156, 272, 411, 870, 959
GsuI	CTGGAG	1 cut(s)	275
HaeIII	GGCC	5 cut(s)	424, 537, 613, 756, 858
HapII	CCGG	9 cut(s)	308, 321, 610, 665, 674, 791, 807, 859, 1004
Hin1II	CATG	5 cut(s)	410, 451, 498, 590, 694
HinfI	GANTC	3 cut(s)	74, 89, 713
HpaII	CCGG	9 cut(s)	308, 321, 610, 665, 674, 791, 807, 859, 1004
HphI	GGTGA	5 cut(s)	196, 297, 788, 842, 896
Hpy166II	GTNNAC	2 cut(s)	796, 812
Hpy188I	TCNGA	2 cut(s)	181, 373
Hpy188III	TCNNGA	3 cut(s)	382, 674, 1069
Hpy8I	GTNNAC	2 cut(s)	796, 812
Hpy99I	CGWCG	2 cut(s)	100, 947
HpyAV	CCTTC	3 cut(s)	121, 526, 1009
HpyCH4III	ACNGT	3 cut(s)	730, 939, 1092
HpyCH4IV	ACGT	1 cut(s)	945
HpyCH4V	TGCA	7 cut(s)	498, 649, 760, 812, 841, 872, 877
HpyF10VI	GCNNNNNNNGC	3 cut(s)	169, 576, 610
HpyF3I	CTNAG	1 cut(s)	180
HpySE526I	ACGT	1 cut(s)	945
Hsp92II	CATG	5 cut(s)	410, 451, 498, 590, 694
Kpn2I	TCCGGA	1 cut(s)	673
Kzo9I	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
LmnI	GCTCC	2 cut(s)	244, 706
Lsp1109I	GCAGC	2 cut(s)	258, 856
LweI	GCATC	5 cut(s)	475, 552, 828, 886, 918
MaeI	CTAG	1 cut(s)	276
MaeII	ACGT	1 cut(s)	945
MaeIII	GTNAC	3 cut(s)	202, 450, 1006
MalI	GATC	6 cut(s)	304, 389, 803, 974, 984, 1062
MboI	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
MboII	GAAGA	5 cut(s)	187, 494, 538, 602, 961
MhlI	GDGCHC	3 cut(s)	337, 814, 1057
MlsI	TGGCCA	1 cut(s)	424
MluCI	AATT	3 cut(s)	548, 631, 720
MluNI	TGGCCA	1 cut(s)	424
MlyI	GAGTC	3 cut(s)	68, 83, 707
MmeI	TCCRAC	2 cut(s)	351, 876
Mox20I	TGGCCA	1 cut(s)	424
MroI	TCCGGA	1 cut(s)	673
MscI	TGGCCA	1 cut(s)	424
MseI	TTAA	3 cut(s)	230, 317, 597
MslI	CAYNNNNRTG	2 cut(s)	221, 591
Msp20I	TGGCCA	1 cut(s)	424
MspI	CCGG	9 cut(s)	308, 321, 610, 665, 674, 791, 807, 859, 1004
MspR9I	CCNGG	2 cut(s)	293, 807
Mva1269I	GAATGC	2 cut(s)	157, 216
MvaI	CCWGG	1 cut(s)	293
MwoI	GCNNNNNNNGC	3 cut(s)	169, 576, 610
NciI	CCSGG	1 cut(s)	807
NcoI	CCATGG	1 cut(s)	690
NdeII	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
NlaIII	CATG	5 cut(s)	410, 451, 498, 590, 694
NlaIV	GGNNCC	4 cut(s)	281, 678, 702, 789
NmuCI	GTSAC	1 cut(s)	202
NspI	RCATGY	1 cut(s)	410
PcsI	WCGNNNNNNNCGW	1 cut(s)	42
PctI	GAATGC	2 cut(s)	157, 216
Pfl23II	CGTACG	1 cut(s)	939
PinAI	ACCGGT	2 cut(s)	320, 1003
PkrI	GCNGC	5 cut(s)	157, 273, 412, 871, 960
PleI	GAGTC	3 cut(s)	68, 83, 707
PpsI	GAGTC	3 cut(s)	68, 83, 707
Psp6I	CCWGG	1 cut(s)	291
PspGI	CCWGG	1 cut(s)	291
PspLI	CGTACG	1 cut(s)	939
PspN4I	GGNNCC	4 cut(s)	281, 678, 702, 789
PspPI	GGNCC	7 cut(s)	100, 280, 535, 583, 612, 676, 1081
PstI	CTGCAG	1 cut(s)	874
RsaI	GTAC	2 cut(s)	518, 941
RsaNI	GTAC	2 cut(s)	517, 940
RseI	CAYNNNNRTG	2 cut(s)	221, 591
SaqAI	TTAA	3 cut(s)	230, 317, 597
SatI	GCNGC	5 cut(s)	156, 272, 411, 870, 959
Sau3AI	GATC	6 cut(s)	302, 387, 801, 972, 982, 1060
Sau96I	GGNCC	7 cut(s)	100, 280, 535, 583, 612, 676, 1081
SchI	GAGTC	3 cut(s)	68, 83, 707
ScrFI	CCNGG	2 cut(s)	293, 807
SduI	GDGCHC	3 cut(s)	337, 814, 1057
SfaNI	GCATC	5 cut(s)	475, 552, 828, 886, 918
SfcI	CTRYAG	2 cut(s)	870, 928
SinI	GGWCC	5 cut(s)	100, 280, 583, 676, 1081
SmiMI	CAYNNNNRTG	2 cut(s)	221, 591
Sse9I	AATT	3 cut(s)	548, 631, 720
SsiI	CCGC	9 cut(s)	156, 242, 337, 345, 398, 410, 884, 907, 959
SspMI	CTAG	1 cut(s)	276
StyD4I	CCNGG	2 cut(s)	291, 805
StyI	CCWWGG	3 cut(s)	275, 690, 1024
TaaI	ACNGT	3 cut(s)	730, 939, 1092
TaiI	ACGT	1 cut(s)	948
TaqI	TCGA	7 cut(s)	45, 95, 144, 301, 437, 975, 1000
TasI	AATT	3 cut(s)	548, 631, 720
TauI	GCSGC	3 cut(s)	158, 413, 961
Tru1I	TTAA	3 cut(s)	230, 317, 597
Tru9I	TTAA	3 cut(s)	230, 317, 597
TscAI	CASTG	1 cut(s)	879
TseFI	GTSAC	1 cut(s)	202
TseI	GCWGC	2 cut(s)	271, 869
Tsp45I	GTSAC	1 cut(s)	202
TspDTI	ATGAA	4 cut(s)	239, 545, 603, 980
TspGWI	ACGGA	2 cut(s)	113, 1094
TspRI	CASTG	1 cut(s)	879
VneI	GTGCAC	1 cut(s)	810
VpaK11BI	GGWCC	5 cut(s)	100, 280, 583, 676, 1081
XapI	RAATTY	1 cut(s)	631
XceI	RCATGY	1 cut(s)	410
XmaJI	CCTAGG	1 cut(s)	275
XspI	CTAG	1 cut(s)	276

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.