RchiOBHm_Chr2g0089681

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014394
Physical Location & Seq
Reverse (-)
3803922 .. 3804677
756 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ46498

Sequence Viewer

Length: 693 bp
ATGCTTTTGACGATTGACTATCAGATATTAGTCATGTTACCATGTGTGTATGACCATTTTGACCCTGAAGATGCCTACTCATGCAGTAATGTAATGGAGGAGTACCAAAAGAAGATGAAGGGCCTATGTGGAAAATTGATAAGGCTGTTGCTTGGATCATTGGGCTTGGACCAAGAAGATGTTAAATGGCTGAACCGGCCCAAAAAGAGCGGCAGAAATTTTCCACAGGTCTTGCAACTGAACTCGTATCCGGTTTGTCCGGACCCGTCCCGAGCCATGGGGTTGGCTCCTCACACAGACTCCTCAATTTTCACAGTGCTAAACCAAAGCAACATAAGTGGCCTGCAAGTCTTTAAGGATGGGATTGGTTGGGTGCCGGTTCACCCGGTCCCCGGTGCACTTGTAGTCAATGTAGGTGATTTGTTGCACATACTATCCAACGGCCGGTTCAAGACTGCAGTGCATCGTGCGGTTGTCAATGAGCTATATCACCGCATCTCCATCGCCTACTTCTACAGCCCACCGTACGACGTGAAGATATCACCGCCGATGAAGTTGATCGACCAAGATCATCCTCCCCTATATCGACCGGTAACTTGGAAGGAATACCTTGGTATCAAAGGAACACATTTCAACAGGGCACTTGATCTAATCAGGAATGACACGGACCAAGCACCATACATGCATGAATAG

Protein Analysis

230

Amino Acids

26.13

Weight (kDa)

7.11

Isoelectric Point (pI)

47.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2OG-FeII_Oxy PF03171 76 - 174 7.6e-34 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014394)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21690
fragaria_vesca FvH4_1g04200
malus_domestica MD15G1145200.v1.1
prunus_persica Prupe.7G235400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0089681 RchiOBHm_Chr2g0089691
rosa_laevigata RLG00000016051
rosa_multiflora Rmu_sc0000665.1_g000022
rosa_roxburghii Rroxscaffold_2G00151670
rosa_rugosa Rorug02G0002600
rosa_samantha Rh2AG048000 Rh2BG046600 Rh2BG046700 Rh2CG048600 Rh2CG048800 Rh2DG048000
rosa_wichuraiana Rw2G004250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 373
AccBSI CCGCTC 1 cut(s) 210
AccIII TCCGGA 1 cut(s) 259
AciI CCGC 4 cut(s) 210, 470, 493, 545
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 442
AcsI RAATTY 1 cut(s) 217
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 2 cut(s) 104, 527
AfiI CCNNNNNNNGG 3 cut(s) 277, 392, 444
AgeI ACCGGT 1 cut(s) 589
AgsI TTSAA 2 cut(s) 451, 634
AjiI CACGTC 1 cut(s) 532
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 1 cut(s) 484
AluI AGCT 1 cut(s) 484
Alw21I GWGCWC 1 cut(s) 400
Alw44I GTGCAC 1 cut(s) 396
AlwI GGATC 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 259
AoxI GGCC 4 cut(s) 121, 197, 340, 442
ApaLI GTGCAC 1 cut(s) 396
ApoI RAATTY 1 cut(s) 217
AsiGI ACCGGT 1 cut(s) 589
AspS9I GGNCC 6 cut(s) 121, 169, 198, 262, 388, 667
AsuC2I CCSGG 2 cut(s) 386, 393
AsuHPI GGTGA 4 cut(s) 374, 428, 482, 534
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 4 cut(s) 169, 262, 388, 667
BaeGI GKGCMC 2 cut(s) 400, 643
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BanI GGYRCC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 400
BccI CCATC 2 cut(s) 353, 509
BceAI ACGGC 1 cut(s) 457
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciVI GTATCC 1 cut(s) 258
BcnI CCSGG 2 cut(s) 386, 393
BfmI CTRYAG 2 cut(s) 456, 514
BfuI GTATCC 1 cut(s) 258
BisI GCNGC 1 cut(s) 211
BlsI GCNGC 1 cut(s) 212
Bme1390I CCNGG 2 cut(s) 386, 393
Bme18I GGWCC 4 cut(s) 169, 262, 388, 667
BmeT110I CYCGRG 1 cut(s) 270
BmgBI CACGTC 1 cut(s) 532
BmgT120I GGNCC 6 cut(s) 121, 169, 198, 262, 388, 667
BmiI GGNNCC 4 cut(s) 264, 288, 375, 390
BmrFI CCNGG 2 cut(s) 386, 393
BmsI GCATC 3 cut(s) 61, 472, 504
BpuMI CCSGG 2 cut(s) 386, 393
BsaJI CCNNGG 3 cut(s) 276, 391, 610
BsaWI WCCGGW 3 cut(s) 250, 259, 589
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 482, 512
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 392, 444
Bse118I RCCGGY 4 cut(s) 195, 376, 444, 589
BseAI TCCGGA 1 cut(s) 259
BseDI CCNNGG 3 cut(s) 276, 391, 610
BseGI GGATG 2 cut(s) 364, 571
BseLI CCNNNNNNNGG 3 cut(s) 277, 392, 444
BseRI GAGGAG 3 cut(s) 113, 279, 292
BseSI GKGCMC 2 cut(s) 400, 643
BseX3I CGGCCG 1 cut(s) 442
Bsh1285I CGRYCG 2 cut(s) 445, 590
BshFI GGCC 4 cut(s) 123, 199, 342, 444
BshNI GGYRCC 1 cut(s) 373
BshTI ACCGGT 1 cut(s) 589
BsiEI CGRYCG 2 cut(s) 445, 590
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 8 cut(s) 196, 251, 260, 377, 386, 393, 445, 590
BsiWI CGTACG 1 cut(s) 525
BslFI GGGAC 2 cut(s) 253, 374
BslI CCNNNNNNNGG 3 cut(s) 277, 392, 444
BsmFI GGGAC 2 cut(s) 253, 374
BsnI GGCC 4 cut(s) 123, 199, 342, 444
BsoBI CYCGRG 1 cut(s) 270
Bsp1286I GDGCHC 2 cut(s) 400, 643
Bsp13I TCCGGA 1 cut(s) 259
Bsp143I GATC 4 cut(s) 155, 558, 568, 646
Bsp19I CCATGG 1 cut(s) 276
BspACI CCGC 4 cut(s) 210, 470, 493, 545
BspANI GGCC 4 cut(s) 123, 199, 342, 444
BspEI TCCGGA 1 cut(s) 259
BspLI GGNNCC 4 cut(s) 264, 288, 375, 390
BspMAI CTGCAG 1 cut(s) 460
BspPI GGATC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 210
BsrFI RCCGGY 4 cut(s) 195, 376, 444, 589
BssAI RCCGGY 4 cut(s) 195, 376, 444, 589
BssECI CCNNGG 3 cut(s) 276, 391, 610
BssMI GATC 4 cut(s) 155, 558, 568, 646
BssT1I CCWWGG 2 cut(s) 276, 610
Bst4CI ACNGT 2 cut(s) 316, 525
BstC8I GCNNGC 1 cut(s) 344
BstDSI CCRYGG 1 cut(s) 276
BstF5I GGATG 2 cut(s) 364, 571
BstKTI GATC 4 cut(s) 158, 561, 571, 649
BstMBI GATC 4 cut(s) 155, 558, 568, 646
BstMCI CGRYCG 2 cut(s) 445, 590
BstMWI GCNNNNNNNGC 1 cut(s) 196
BstNSI RCATGY 1 cut(s) 685
BstSCI CCNGG 2 cut(s) 384, 391
BstSFI CTRYAG 2 cut(s) 456, 514
BstSLI GKGCMC 2 cut(s) 400, 643
BstXI CCANNNNNNTGG 1 cut(s) 283
BstZI CGGCCG 1 cut(s) 442
BsuI GTATCC 1 cut(s) 258
BsuRI GGCC 4 cut(s) 123, 199, 342, 444
BtgI CCRYGG 1 cut(s) 276
BtgZI GCGATG 1 cut(s) 487
BtrI CACGTC 1 cut(s) 532
BtsCI GGATG 2 cut(s) 364, 571
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 2 cut(s) 321, 465
Cac8I GCNNGC 1 cut(s) 344
Cfr10I RCCGGY 4 cut(s) 195, 376, 444, 589
Cfr13I GGNCC 6 cut(s) 121, 169, 198, 262, 388, 667
Csp6I GTAC 2 cut(s) 103, 526
CspAI ACCGGT 1 cut(s) 589
CspCI CAANNNNNGTGG 4 cut(s) 213, 248, 319, 354
CviAII CATG 6 cut(s) 34, 42, 81, 277, 682, 686
CviQI GTAC 2 cut(s) 103, 526
DpnI GATC 4 cut(s) 157, 560, 570, 648
DpnII GATC 4 cut(s) 155, 558, 568, 646
EaeI YGGCCR 1 cut(s) 442
EagI CGGCCG 1 cut(s) 442
EclXI CGGCCG 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 276, 610
Eco32I GATATC 1 cut(s) 540
Eco47I GGWCC 4 cut(s) 169, 262, 388, 667
Eco52I CGGCCG 1 cut(s) 442
Eco57I CTGAAG 1 cut(s) 87
Eco88I CYCGRG 1 cut(s) 270
EcoO109I RGGNCCY 1 cut(s) 121
EcoRV GATATC 1 cut(s) 540
EcoT14I CCWWGG 2 cut(s) 276, 610
EcoT22I ATGCAT 1 cut(s) 687
ErhI CCWWGG 2 cut(s) 276, 610
FaeI CATG 6 cut(s) 37, 45, 84, 280, 685, 689
FaqI GGGAC 2 cut(s) 253, 374
FatI CATG 6 cut(s) 33, 41, 80, 276, 681, 685
Fnu4HI GCNGC 1 cut(s) 211
FokI GGATG 2 cut(s) 371, 558
Fsp4HI GCNGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 211
HaeIII GGCC 4 cut(s) 123, 199, 342, 444
HapII CCGG 8 cut(s) 196, 251, 260, 377, 386, 393, 445, 590
Hin1II CATG 6 cut(s) 37, 45, 84, 280, 685, 689
HinfI GANTC 1 cut(s) 299
HpaII CCGG 8 cut(s) 196, 251, 260, 377, 386, 393, 445, 590
HphI GGTGA 4 cut(s) 374, 428, 482, 534
Hpy166II GTNNAC 2 cut(s) 382, 398
Hpy188I TCNGA 1 cut(s) 24
Hpy188III TCNNGA 4 cut(s) 260, 270, 451, 655
Hpy8I GTNNAC 2 cut(s) 382, 398
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 2 cut(s) 112, 595
HpyCH4III ACNGT 2 cut(s) 316, 525
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 8 cut(s) 84, 235, 346, 398, 427, 458, 463, 685
HpyF10VI GCNNNNNNNGC 1 cut(s) 196
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 6 cut(s) 37, 45, 84, 280, 685, 689
Kpn2I TCCGGA 1 cut(s) 259
Kzo9I GATC 4 cut(s) 155, 558, 568, 646
LmnI GCTCC 1 cut(s) 292
LweI GCATC 3 cut(s) 61, 472, 504
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 2 cut(s) 36, 592
MalI GATC 4 cut(s) 157, 560, 570, 648
MbiI CCGCTC 1 cut(s) 210
MboI GATC 4 cut(s) 155, 558, 568, 646
MboII GAAGA 4 cut(s) 80, 124, 188, 547
MhlI GDGCHC 2 cut(s) 400, 643
MluCI AATT 3 cut(s) 134, 217, 306
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 4 cut(s) 91, 300, 313, 585
Mph1103I ATGCAT 1 cut(s) 687
MroI TCCGGA 1 cut(s) 259
MseI TTAA 2 cut(s) 183, 354
MspI CCGG 8 cut(s) 196, 251, 260, 377, 386, 393, 445, 590
MspR9I CCNGG 2 cut(s) 386, 393
MwoI GCNNNNNNNGC 1 cut(s) 196
NciI CCSGG 2 cut(s) 386, 393
NcoI CCATGG 1 cut(s) 276
NdeII GATC 4 cut(s) 155, 558, 568, 646
NlaIII CATG 6 cut(s) 37, 45, 84, 280, 685, 689
NlaIV GGNNCC 4 cut(s) 264, 288, 375, 390
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 1 cut(s) 685
Pfl23II CGTACG 1 cut(s) 525
PinAI ACCGGT 1 cut(s) 589
PkrI GCNGC 1 cut(s) 212
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PspLI CGTACG 1 cut(s) 525
PspN4I GGNNCC 4 cut(s) 264, 288, 375, 390
PspPI GGNCC 6 cut(s) 121, 169, 198, 262, 388, 667
PstI CTGCAG 1 cut(s) 460
RsaI GTAC 2 cut(s) 104, 527
RsaNI GTAC 2 cut(s) 103, 526
SaqAI TTAA 2 cut(s) 183, 354
SatI GCNGC 1 cut(s) 211
Sau3AI GATC 4 cut(s) 155, 558, 568, 646
Sau96I GGNCC 6 cut(s) 121, 169, 198, 262, 388, 667
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 2 cut(s) 386, 393
SduI GDGCHC 2 cut(s) 400, 643
SetI ASST 5 cut(s) 231, 418, 486, 534, 612
SfaNI GCATC 3 cut(s) 61, 472, 504
SfcI CTRYAG 2 cut(s) 456, 514
SinI GGWCC 4 cut(s) 169, 262, 388, 667
Sse9I AATT 3 cut(s) 134, 217, 306
SsiI CCGC 4 cut(s) 210, 470, 493, 545
StyD4I CCNGG 2 cut(s) 384, 391
StyI CCWWGG 2 cut(s) 276, 610
TaaI ACNGT 2 cut(s) 316, 525
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 561, 586
TasI AATT 3 cut(s) 134, 217, 306
TauI GCSGC 1 cut(s) 213
Tru1I TTAA 2 cut(s) 183, 354
Tru9I TTAA 2 cut(s) 183, 354
TscAI CASTG 2 cut(s) 321, 465
TspDTI ATGAA 2 cut(s) 131, 566
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 2 cut(s) 321, 465
VneI GTGCAC 1 cut(s) 396
VpaK11BI GGWCC 4 cut(s) 169, 262, 388, 667
XapI RAATTY 1 cut(s) 217
XceI RCATGY 1 cut(s) 685
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.