MD15G1154600.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009533
Physical Location & Seq
Reverse (-)
11461835 .. 11464849
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1154600.v1.1.491

Sequence Viewer

Length: 714 bp
ATGAACCAAGCTCCGGCGGGTGGGTCGGCTGGGGCTGCGCCGGCCCAACCCAAGCCCAGAGTCAGGGCGAGAAGAGGTCAAGCCACTGACCCACACAGCATAGCCGAAAGATTACGGCGAGAGAGAATTGCAGAGAGAATGAAGGCTCTGCAGGAGCTGGTTCCCAACGCCAACAAGACGGACAAAGCTTCAATGCTGGATGAGATAATCGATTATGTAAAATTCCTCCAGCTCCAAGTCAAGGTTCTGAGCATGAGCAGGTTGGGCGGTGCAGCTGCTGTTGCTCCCCTTGTTGCTGATATGTCCTCTGAGGGTGGCGGTGACTGTATCCAAGCCAGTGCCAACGGCGGGACCCGCGGACGGAGCTCAAACGGCAACCAAACGGCGTCATCATCCAACGACAACAGCATGACGGTGACGGAACACCAAGTCGCCAAGCTCATGGAGGAAGACATGGGCTCCGCCATGCAGTACCTCCAGGGAAAAGGCCTCTGCCTCATGCCGATTTCCTTGGCCACCGCCATCTCCACAGCCACATGTCACTCCAGGAACCCCCTCCTCCAGAACAGTAACAACAACCCGGTCATCCCGTCCAACGGTGGCGACGGGCCATCTTCACCCAGCATGTCCGTGCTGACCGTCCAGTCAGCCACCATGGGTAACGGCGGGGTTGAGGGTTCCGTCAAAGACGCCACCTCCGTTTCCAAGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009555 GO:0009653 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016049 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019953 GO:0021700 GO:0022412 GO:0022414 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032504 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0040007 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0044703 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048229 GO:0048232 GO:0048235 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048588 GO:0048589 GO:0048609 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0055046 GO:0060255 GO:0060560 GO:0065007 GO:0071695 GO:0071704 GO:0080090 GO:0080147 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

238

Amino Acids

24.55

Weight (kDa)

8.84

Isoelectric Point (pI)

51.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 32 - 77 3.1e-10 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 36 - 80 8.2e-07 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 643
Acc36I ACCTGC 1 cut(s) 249
AccII CGCG 1 cut(s) 357
AciI CCGC 9 cut(s) 17, 267, 318, 348, 355, 357, 462, 519, 666
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 1 cut(s) 221
AcyI GRCGYC 2 cut(s) 386, 690
AfaI GTAC 1 cut(s) 473
AfiI CCNNNNNNNGG 7 cut(s) 13, 20, 63, 241, 348, 360, 596
AflIII ACRYGT 1 cut(s) 536
AgsI TTSAA 1 cut(s) 192
AjnI CCWGG 2 cut(s) 477, 545
AjuI GAANNNNNNNTTGG 2 cut(s) 228, 260
AluBI AGCT 7 cut(s) 11, 157, 188, 232, 275, 366, 439
AluI AGCT 7 cut(s) 11, 157, 188, 232, 275, 366, 439
Alw21I GWGCWC 1 cut(s) 368
AlwNI CAGNNNCTG 2 cut(s) 157, 278
AoxI GGCC 4 cut(s) 42, 487, 513, 608
ApeKI GCWGC 3 cut(s) 35, 272, 275
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 1 cut(s) 40
AspS9I GGNCC 3 cut(s) 43, 351, 608
AsuC2I CCSGG 1 cut(s) 581
AsuHPI GGTGA 3 cut(s) 332, 427, 609
AvaII GGWCC 1 cut(s) 351
BalI TGGCCA 1 cut(s) 515
BanII GRGCYC 2 cut(s) 368, 461
BbsI GAAGAC 1 cut(s) 456
Bbv12I GWGCWC 1 cut(s) 368
BbvI GCAGC 3 cut(s) 22, 262, 284
BccI CCATC 2 cut(s) 530, 619
BceAI ACGGC 5 cut(s) 131, 361, 388, 399, 679
BciT130I CCWGG 2 cut(s) 479, 547
BciVI GTATCC 1 cut(s) 338
BcnI CCSGG 1 cut(s) 581
BfmI CTRYAG 1 cut(s) 149
BfuAI ACCTGC 1 cut(s) 249
BfuI GTATCC 1 cut(s) 338
BisI GCNGC 3 cut(s) 36, 273, 276
BlsI GCNGC 3 cut(s) 37, 274, 277
Bme1390I CCNGG 3 cut(s) 479, 547, 581
Bme18I GGWCC 1 cut(s) 351
BmgT120I GGNCC 3 cut(s) 43, 351, 608
BmiI GGNNCC 6 cut(s) 162, 352, 353, 460, 551, 679
BmrFI CCNGG 3 cut(s) 479, 547, 581
BpiI GAAGAC 1 cut(s) 456
BpmI CTGGAG 4 cut(s) 212, 461, 529, 545
BpuMI CCSGG 1 cut(s) 581
Bsa29I ATCGAT 1 cut(s) 210
BsaHI GRCGYC 2 cut(s) 386, 690
BsaJI CCNNGG 4 cut(s) 355, 478, 510, 654
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 680, 710
Bsc4I CCNNNNNNNGG 7 cut(s) 13, 20, 63, 241, 348, 360, 596
Bse118I RCCGGY 1 cut(s) 40
Bse1I ACTGG 2 cut(s) 336, 643
BseBI CCWGG 2 cut(s) 479, 547
BseCI ATCGAT 1 cut(s) 210
BseDI CCNNGG 4 cut(s) 355, 478, 510, 654
BseGI GGATG 3 cut(s) 205, 392, 585
BseLI CCNNNNNNNGG 7 cut(s) 13, 20, 63, 241, 348, 360, 596
BseMII CTCAG 2 cut(s) 239, 300
BseNI ACTGG 2 cut(s) 336, 643
BseRI GAGGAG 1 cut(s) 548
BseXI GCAGC 3 cut(s) 22, 262, 284
BseYI CCCAGC 2 cut(s) 29, 620
BsgI GTGCAG 1 cut(s) 291
Bsh1236I CGCG 1 cut(s) 357
BshFI GGCC 4 cut(s) 44, 489, 515, 610
BshVI ATCGAT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 368
BsiSI CCGG 3 cut(s) 14, 41, 581
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 7 cut(s) 13, 20, 63, 241, 348, 360, 596
BsmFI GGGAC 1 cut(s) 364
BsnI GGCC 4 cut(s) 44, 489, 515, 610
Bsp1286I GDGCHC 2 cut(s) 368, 461
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 9 cut(s) 17, 267, 318, 348, 355, 357, 462, 519, 666
BspANI GGCC 4 cut(s) 44, 489, 515, 610
BspCNI CTCAG 2 cut(s) 240, 301
BspDI ATCGAT 1 cut(s) 210
BspFNI CGCG 1 cut(s) 357
BspLI GGNNCC 6 cut(s) 162, 352, 353, 460, 551, 679
BspMAI CTGCAG 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 249
BsrFI RCCGGY 1 cut(s) 40
BsrI ACTGG 2 cut(s) 336, 643
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 4 cut(s) 355, 478, 510, 654
BssNI GRCGYC 2 cut(s) 386, 690
BssT1I CCWWGG 2 cut(s) 510, 654
Bst2UI CCWGG 2 cut(s) 479, 547
Bst4CI ACNGT 5 cut(s) 326, 415, 569, 599, 640
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 2 cut(s) 386, 690
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 2 cut(s) 248, 309
BstDSI CCRYGG 2 cut(s) 355, 654
BstF5I GGATG 3 cut(s) 205, 392, 585
BstFNI CGCG 1 cut(s) 357
BstHHI GCGC 1 cut(s) 40
BstMWI GCNNNNNNNGC 7 cut(s) 35, 41, 264, 281, 354, 363, 372
BstNI CCWGG 2 cut(s) 479, 547
BstNSI RCATGY 2 cut(s) 540, 628
BstSCI CCNGG 3 cut(s) 477, 545, 579
BstSFI CTRYAG 1 cut(s) 149
BstUI CGCG 1 cut(s) 357
BstV1I GCAGC 3 cut(s) 22, 262, 284
BstV2I GAAGAC 1 cut(s) 456
BstXI CCANNNNNNTGG 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 210
BsuI GTATCC 1 cut(s) 338
BsuRI GGCC 4 cut(s) 44, 489, 515, 610
BsuTUI ATCGAT 1 cut(s) 210
BtgI CCRYGG 2 cut(s) 355, 654
BtsCI GGATG 3 cut(s) 205, 392, 585
BtsIMutI CAGTG 2 cut(s) 84, 343
BveI ACCTGC 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 42
CaiI CAGNNNCTG 2 cut(s) 157, 278
CfoI GCGC 1 cut(s) 40
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 3 cut(s) 43, 351, 608
Cfr42I CCGCGG 1 cut(s) 358
ClaI ATCGAT 1 cut(s) 210
CseI GACGC 2 cut(s) 375, 698
Csp6I GTAC 1 cut(s) 472
CviAII CATG 9 cut(s) 253, 409, 442, 454, 466, 499, 537, 625, 655
CviQI GTAC 1 cut(s) 472
DdeI CTNAG 2 cut(s) 248, 309
DrdI GACNNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 643
EaeI YGGCCR 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 451
Ecl136II GAGCTC 1 cut(s) 366
Eco130I CCWWGG 2 cut(s) 510, 654
Eco147I AGGCCT 1 cut(s) 489
Eco24I GRGCYC 2 cut(s) 368, 461
Eco47I GGWCC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoO109I RGGNCCY 1 cut(s) 351
EcoRII CCWGG 2 cut(s) 477, 545
EcoT14I CCWWGG 2 cut(s) 510, 654
EcoT38I GRGCYC 2 cut(s) 368, 461
ErhI CCWWGG 2 cut(s) 510, 654
FaeI CATG 9 cut(s) 256, 412, 445, 457, 469, 502, 540, 628, 658
FaqI GGGAC 1 cut(s) 364
FatI CATG 9 cut(s) 252, 408, 441, 453, 465, 498, 536, 624, 654
FauI CCCGC 4 cut(s) 10, 341, 362, 659
Fnu4HI GCNGC 3 cut(s) 36, 273, 276
FokI GGATG 3 cut(s) 212, 379, 572
FriOI GRGCYC 2 cut(s) 368, 461
Fsp4HI GCNGC 3 cut(s) 36, 273, 276
GlaI GCGC 1 cut(s) 39
GluI GCNGC 3 cut(s) 36, 273, 276
GsaI CCCAGC 2 cut(s) 33, 624
GsuI CTGGAG 4 cut(s) 212, 461, 529, 545
HaeIII GGCC 4 cut(s) 44, 489, 515, 610
HapII CCGG 3 cut(s) 14, 41, 581
HgaI GACGC 2 cut(s) 375, 698
HhaI GCGC 1 cut(s) 40
Hin1I GRCGYC 2 cut(s) 386, 690
Hin1II CATG 9 cut(s) 256, 412, 445, 457, 469, 502, 540, 628, 658
Hin6I GCGC 1 cut(s) 38
HinP1I GCGC 1 cut(s) 38
HindIII AAGCTT 1 cut(s) 186
HinfI GANTC 1 cut(s) 60
HpaII CCGG 3 cut(s) 14, 41, 581
HphI GGTGA 3 cut(s) 332, 427, 609
Hpy188I TCNGA 2 cut(s) 249, 310
Hpy188III TCNNGA 1 cut(s) 562
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 1 cut(s) 136
HpyCH4III ACNGT 5 cut(s) 326, 415, 569, 599, 640
HpyCH4V TGCA 4 cut(s) 131, 151, 272, 469
HpyF10VI GCNNNNNNNGC 7 cut(s) 35, 41, 264, 281, 354, 363, 372
HpyF3I CTNAG 2 cut(s) 248, 309
Hsp92I GRCGYC 2 cut(s) 386, 690
Hsp92II CATG 9 cut(s) 256, 412, 445, 457, 469, 502, 540, 628, 658
HspAI GCGC 1 cut(s) 38
KflI GGGWCCC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspI CCGCGG 1 cut(s) 358
LmnI GCTCC 6 cut(s) 16, 154, 237, 289, 363, 464
Lsp1109I GCAGC 3 cut(s) 22, 262, 284
MaeIII GTNAC 5 cut(s) 320, 415, 539, 569, 659
MboII GAAGA 3 cut(s) 84, 461, 606
MhlI GDGCHC 2 cut(s) 368, 461
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 2 cut(s) 126, 221
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 1 cut(s) 69
MmeI TCCRAC 2 cut(s) 420, 618
Mox20I TGGCCA 1 cut(s) 515
MroNI GCCGGC 1 cut(s) 40
MscI TGGCCA 1 cut(s) 515
MslI CAYNNNNRTG 2 cut(s) 413, 629
Msp20I TGGCCA 1 cut(s) 515
MspA1I CMGCKG 2 cut(s) 275, 357
MspI CCGG 3 cut(s) 14, 41, 581
MspR9I CCNGG 3 cut(s) 479, 547, 581
MvaI CCWGG 2 cut(s) 479, 547
MvnI CGCG 1 cut(s) 357
MwoI GCNNNNNNNGC 7 cut(s) 35, 41, 264, 281, 354, 363, 372
NaeI GCCGGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 581
NcoI CCATGG 1 cut(s) 654
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 9 cut(s) 256, 412, 445, 457, 469, 502, 540, 628, 658
NlaIV GGNNCC 6 cut(s) 162, 352, 353, 460, 551, 679
NmuCI GTSAC 3 cut(s) 320, 415, 539
NspI RCATGY 2 cut(s) 540, 628
PceI AGGCCT 1 cut(s) 489
PciI ACATGT 1 cut(s) 536
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PdiI GCCGGC 1 cut(s) 42
PfoI TCCNGGA 1 cut(s) 545
PkrI GCNGC 3 cut(s) 37, 274, 277
PleI GAGTC 1 cut(s) 68
PpsI GAGTC 1 cut(s) 68
PpuMI RGGWCCY 1 cut(s) 351
PscI ACATGT 1 cut(s) 536
Psp124BI GAGCTC 1 cut(s) 368
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 2 cut(s) 477, 545
PspFI CCCAGC 2 cut(s) 29, 620
PspGI CCWGG 2 cut(s) 477, 545
PspN4I GGNNCC 6 cut(s) 162, 352, 353, 460, 551, 679
PspPI GGNCC 3 cut(s) 43, 351, 608
PspPPI RGGWCCY 1 cut(s) 351
PstI CTGCAG 1 cut(s) 153
PstNI CAGNNNCTG 2 cut(s) 157, 278
PvuII CAGCTG 1 cut(s) 275
RsaI GTAC 1 cut(s) 473
RsaNI GTAC 1 cut(s) 472
RseI CAYNNNNRTG 2 cut(s) 413, 629
SacI GAGCTC 1 cut(s) 368
SacII CCGCGG 1 cut(s) 358
SatI GCNGC 3 cut(s) 36, 273, 276
Sau96I GGNCC 3 cut(s) 43, 351, 608
SchI GAGTC 1 cut(s) 69
ScrFI CCNGG 3 cut(s) 479, 547, 581
SduI GDGCHC 2 cut(s) 368, 461
SfcI CTRYAG 1 cut(s) 149
Sfr303I CCGCGG 1 cut(s) 358
SgrBI CCGCGG 1 cut(s) 358
SinI GGWCC 1 cut(s) 351
SmiMI CAYNNNNRTG 2 cut(s) 413, 629
Sse9I AATT 2 cut(s) 126, 221
SseBI AGGCCT 1 cut(s) 489
SsiI CCGC 9 cut(s) 17, 267, 318, 348, 355, 357, 462, 519, 666
SstI GAGCTC 1 cut(s) 368
StuI AGGCCT 1 cut(s) 489
StyD4I CCNGG 3 cut(s) 477, 545, 579
StyI CCWWGG 2 cut(s) 510, 654
TaaI ACNGT 5 cut(s) 326, 415, 569, 599, 640
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 126, 221
TscAI CASTG 2 cut(s) 91, 343
TseFI GTSAC 3 cut(s) 320, 415, 539
TseI GCWGC 3 cut(s) 35, 272, 275
Tsp45I GTSAC 3 cut(s) 320, 415, 539
TspDTI ATGAA 2 cut(s) 17, 155
TspGWI ACGGA 6 cut(s) 194, 376, 434, 619, 670, 688
TspRI CASTG 2 cut(s) 91, 343
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 1 cut(s) 221
XceI RCATGY 2 cut(s) 540, 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.