Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010166

Physical Location & Seq

Genomic Coordinates Reverse (-)

14382229 .. 14395814

Gene Structure 13586 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1181200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7764 bp

ATGGACAGCTCCAATAACTCCTCCAATTCTTCTACCCGTTCTGCCCCCGCCGTCGTCGAGGAGCCGGAGTACCTCGCCCGCTACTTGGTCATCAAACACTCGTGGCGTGGCCGCTACAAGCGATTTCTCTGCCTCTCGAACGTCGCCATAACCACCTTGGACCCCGCCACGCTCTCCGTCACCAATTCCTACGACGTCGCCACCGATTTTGACTCCGCCTCGCCGATCATCAGCCGCGACGAGAACTCCAACGAGTTCAATTTGAGTGTTCGGACGGACGGACGGGGGAAATTCAAAGGCATTAAGTTCTCGTCTAGGTACAGAGCGAGTATACTGACCGAGTTGCACAGGATTAGGAGGAATCGGTTTGGCGCCGTCGCTGAGTTTCCGGTGCTTCATCTCCGGCGCCGGAATGCCGAGTGGGTCCCACTCAAATTAAAAGTTACTTATGTTGGGGTTGAACTTATTGATTTAAAAAGTGGAGACCTCCGTTGGTGCTTGGATTTCAGAGATTTTGATTCACCTGCCATTGTTTCCCTTTCTGATGCTTATGGAAAGAAAGGCGGAGAACATGGAAGTTTCGTTCTTTGTCCTCTATATGGAAGAAAATCTAAAGCTTTTCAAGCTGCTTCTGGAACCACGAATTCAGTTATCATTGCAAGTTTGACCAAAACAGCAAAATCTATGGTGGGGTTGTCACTAACTGTGGACACTTCTCAGTCTCTCACTATACCAGAGTACATAAAGCGAAGGGCAAAAGAGGCAGTTGGAGCAGAAGAAACTCCGTGTGGAGGTTGGTCTGTGACAAGGTTGCGCTCTGCTGCTCGTGGAACTTTAAATGTTCCAGGGTTGAGTTTGAGTGTTGGCCCAAAAGGTGGACTTGGGGAGAATGGTGATGCTGTATCTCGTCAACTGATTCTTACAAAAGTTTCTCTTGTTGAAAGACGCCCAGAGAACTATGAAGCTGTTATTGTTCGACCTTTATCTGCAGTATACGCACTTGTTCGATTTGCTGAGGAGCCCCAAATGTTTGCAATTGAGTTCAATGATGGGTGTCCCATCCATGTTTATGCAAGCACGTCTCGTGATAGCCTACTGGCAGCAGTTCGGGATCTATTGCAAACAGAAGGCCAATGTTCTGTGACTGTGTTACCAAGATTGACAATGCCTGGGCATGGCATTGATCCACCTTGCGGGAGAGTCCATTTACAATTTGGACTCCAGCGGCTTGTTGCAGATATGGAGAGTGCATCTATGCATTTGAAACACTTAGCAGCAGCTGCCAAAGATGCAGTTTCTGAAGGTGGTTCTATTCCTGGATCACGAGCTAAGCTATGGCGTAGAATAAGAGAGTTCAATGCATGTATACCATATAGTGGAGTTCCTCCGAACATAGAAGTACCTGAGGTGACTTTGATGGCTTTAATAACTATGCTTCCATCTACGCCAAATCTTCCTCCAGAGTCCCCTCCCTTACCTCCCCCTTCACCTAAAGCGGCTGCGACGGTGATGGGCTTTATTGCATGCTTACGTAGATTACTAGCTTCAAGAACTGCTGCTTCACATGTCATGTCTTTTCCTGCTGCTGTTGGAAGAATAATGGGCTTACTCAGAAATGGTTCAGAGGGTGTAGCTGCTGAAGCAGCAGGCCTTGTCGCAGTACTTATTGGTGGTGGTCCCGGGGATACAAATATACTAACGGATTCTAAAGGAGAGCAGCATGCCACAATTATGCACACAAAGTCAGTGTTGTTTGCCAATCATGGTTATGCTATTATACTCTCCAACAGACTTAAGCCTATGTCTGTATCACCATTATTGTCAATGGCTATTGTTGAGGTTCTTGAGGCTATGATATGTGAACCACATGGTGAGACAACACAATACACAGTGTTTGTTGAACTGTTACGCCAAGTAGCTGGTTTGAAGCGTCGTTTGTTTGCATTGTTTGGACATCCTGCTGAAAGTGTTAGGGAAACAGTGGCTGTGATCATGCGTACAATTGCAGAAGAAGATGCAATTGCAGCAGAGTCAATGCGTGATGCTGCATTGCGCGATGGCGCTCTACTGAGGCATTTATTGCATGCGTTCTTCCTTCCTCCTGGTGAGCGACGTGAAGTTAGTCGACAGCTTGTTGCTCTTTGGGCAGATTCTTATCAGCCAGCTCTGGATTTATTGTCTAGAGTTCTGCCTCCTGGGCTTGTTGCTTATTTGCACACACGTTCTGATGGAACTCAATTGGAAGATGCGAATCAAGAAGGATCCTTGACCAGCAGAAGGCAGAGACGCTTACTCCACTTGAGGAAAGGTCGTGCAGGGAGAGGACCAACATCTCAAGAGCATTCCTTACTTAATATAAACAATTCTGAAGTTGGTGATCCCATGACACAGACAGGTGGTGGTGCTTTTAAGGCTGATAACAATCAAAGGTCTGCACTGGTTTCGAGTTCTGGACGGGCTTCTACTCTTCAATCTTCTGTTGCTCAAAGTCAAACCTGTGAAAATTTGACGGCTGAAGTCTTTTCAGGTGTTCCACAAAAGAATCATTCAGCACTTGTGGCGTCAGCTGATATTCAGTCAACGAGCATTCATGAGGCAGTGGAAGCAAATACTTCAATCTCAACTGATTCTGATTCCAATACTACTGGTTTCCAGAGCACAGGCCTCCCTGCTCCTGCTCAGGTTGTTGTCGAAAACACTCCGGTGGGATCCGGCAGGCTACTTTGTAATTGGCCTGAATTCTGGCGAGCATTTAGCCTTGATCACAATCGTGCAGATTTGATCTGGAATGAGCGTACCCGGCAAGAGTTGAGGGAGACTTTGCAGGCTGAGGTTCATAAGTTAGATGTTGAGAAGGAGCGTACTGAAGATATTGTTCCGGGAGGTGCTATGGTAGAGACTGCAACTGGTCAAGATAGCGTGCCACAAATATCTTGGAACTACTCTGAGTTCTCTGTTAGATATCTAAGCTTGTCCAAAGAAGTTTGTGTGGGTCAGTATTATCTGCGTTTACTGCTTGAGAGTGGCAGTGTTGGCAGGGCACAAAATTTTCCGTTGCGCGATCCAGTTGCGTTCTTTAGAGCACTCTACCATCGGTTCTTATGTGATGCAGACATAGGACTTACCGTAGATGGTGCTGTTCCAGATGAAATGGGTGCATCTGATGATTGGTGTGACATGGGAAGACTAGATGGTTTTGGAGGTGGAGGAGGATATTCAGTGAGAGAGCTCTGTGCAAGAGCGATGGCAATTGTGTATGAACAGCATTACAAGACAGTAGGTCCCTTTGAGGGCACTGCTCACATCACAGTCCTTTTGGATAGGACAGATGATAGAGCTTTAAGGCACCGCCTTCTATTGCTCTTGAAGGCGTTAATGAAGGTTTTGTCAAATGTGGAGGCGTGTGTTTTGGTGGGAGGATGTGTGTTAGCTGTTGATATGCTAACAGTAGCTCATGAGGCTTCTGAAAGGACAGCTATTCCTTTGCAATCTAATTTGATTGCTGCTACTGCTTTTATGGAACCGCTCAAGGAATGGATGTTTATTGACAAGGAAGGAGCACAGGTTGGACCTGTTGAAAAGGATGCCATTAGGAGATTTTGGTCAAAGAAGGATATTAATTGGACAACGAGATGCTGGGCTTCTGGGATGTTGGACTGGAAGAGATTGCGGGATATCCGTGAACTTCGTTGGGCTTTAGCTGTTCGAGTCCCTGTTCTCACGCCAACTCAGATTGGTGAGGCAGCATTGTCCATATTACATAGCATGGTATCTGCACATTCAGATTTAGATGATGCTGGAGAGATAGTTACCCCAACTCCTAGAGTAAAATGGATCTTGTCAAGTCCACGCTGCCTTCCACATATTGCTCAGGCTATGCTTTCTGGAGAGCCAAGTATTGTAGAGGGTGCTGCTGCTTTACTTAAGGCCATTGTAACTAGAAATCCCAAGCCCATGATTCGACTATACAGCACAGGGGTATTTTATTTTGCCCTGACATATCCTGGATCTAATCTCCTTTCAATCTCCCAACTCTTCTCATTGACTCATGTTCATCAAGCATTTCATGGTGGTGAAGATGCTGCAGTTTCCTCTTCCTTGCCTCTGGCGAAACGCAGTGTATTGGGTGGGCTTCTTCCTGCATCTTTGTTGTATGTATTGGAACGTAGTGGTCCAGCTGCATTTGCAGCTGCAATGGTATCGGACTCTGATACTCCAGAGATTATATGGACACACAAGATGCGAGCGGAAAATCTGATTCGGCAGGTGTTGCAGCATCTTGGTGACTTTCCTCAGAAATTGTCTCAGCACTGCCATTCTTTATATGAATACGCTCCAATGCCACCAGTGACATACCCAGAGTTAAGAGATGAAATGTGGTGTCACCGTTATTACCTCCGGAACTTGTGTGATGAGATTCGATTTCCAAATTGGCCAATTGTTGAACATGTTGAGTTCCTACAATCATTATTAGTAATGTGGCGTGAGGAGTTGACAAGACGGCCAATGGATCTTTCTGAAGAAGAAGCTTGCAAAATATTAGAGATTTCCTTGGAAGATGTATCAAATGATGATGCCAACACAAGGCATTCTTTTGAGATGGGTGAGGAGATGCCTAGCATAACCAAGCAGATTGAGAACATTGATGAAGAAAAGCTTAAACGGCAATATAGGAAACTTGCCATGAGATACCATCCAGACAAAAATCCTGAAGGAAGGGATAAGTTTCTTGCTGTACAGAAAGCTTATGAGCGCCTGCAGGCCACTATGCAAGGCTTGCAAGGCCCTCAGCCTTGGCGACTGTTGCTTTTATTGAAAGGACAGTGTATCTTGTACAGACGCTATGGAGTCATTCTGGAGCCATTTAAATATGCTGGTTATCCAATGTTGCTCAATGCAGTTACTGTGGACAAGGATGATAACAATTTTCTTTCCTCAGATAGATCACCTCTCCTGGTTGCGGCATCAGAGCTTATTTGGCTGACGTGTGCATCTTCTTCATTGAATGGAGAAGAGCTTGTGAGGGATGGTGGCATACAACTTCTTGCAAATCTTCTTTCCCGTTGCATTTGCGTAGTTCAGCCGAGCACTGCTGCAAGTGAACCATCTGCCATAATTGTTACAAATGTGATGCGAACCTTTTGTGTTTTGAGTATATTTGAGAGTGCCTGGGCTGAGATCCTTGAATATTCTGGACTAGTTGATGACATTGTGCATTGCACTGAACTTGAGCTTGTACCTTCTGCTGTCGATGCTGCTGTCCAGACTATTGCACATGTTTCTGTGTCCACTGAATTGCAGGATGCTTTGCTGAAGGCTGGTGTCTTATGGTACCTTTTGCCCTTGCTTCTTCAGTATGACTCCACTGCAGAGGAATCTGATGCAACAGAATCACATGGAGTTGGTGCTAGTGTACAAATTGCCAAAAATATGCATGCTGTACGTGCCTCGCAGGCACTATCAAGGCTTAGTGGCTTGTGTAGTGATGAGAGTACAACACTTTATAATCAGACTGCAGCTGCTGCCCTCAGAGCTTTGTTGACACCTAAGCTTGCCAGTATGTTAAAAGATCAAGCACCGAAAGACCTACTATCTAAACTAAACAATAACTTGGAGTCACCAGAGATTATCTGGAACTCCTCAACCCGAGCAGAACTACTGAAATTTGTGGATCAGCAACGTGCAAGTCAGGGTCCTGATGGTTCATATGATATGAAAGATTCACATCTGTTTGGGTATAAGGCACTATCAAAAGAACTATATGTTGGCAATGTTTACTTGAGGGTCTATAACAATCAACCAGATTCTGAGATCAGTGAACCAGAGGCTTTCTGTGTTGCATTAGTTGATTTTATAGCATATCTTGTGCACAATCAATGTGCGACTGATTCTGAAATTAAGGATGTACCAAATCAGAATGGCTCATCCCTTGAGACACCTGAGGATTCTAATGATACGGCTATTGGTTCAACTGATGAACAGAATACTCCGGCTGAAGATTCTGCACTATCTAATGGACAAGTAGTAGATAAGGAAGAATTTGAAGCTGCTAAGAACCTTAAATTTGCATTGAATTCACTTAAGAACTTACTGACAAGCAGTCCAAATTTGGCGTCAATTTTTTCTACAAAAGATAAGTTATTGCCTCTTTTCGGATGCTTTTCTGTGCCTGTTGCATCAGAAAGCAACATTCCTCAACTTTGCCTGAGTGTGTTGTCACTCTTGACTACATATGCTTCCTGCTTGGAGGCTATGGTTGCCGATGGATCAAGCCTTCTTCTTTTATTACAAATGCTTCACTCAGCCCCAACTTGTCGTGAAGGGGTTCTTCATGTTCTTTATGCTCTGGCCAGCACACCTGAACTTGCTTGGGCAGCTGCCAAGCATGGGGGAGTTGTCTACATCCTTGAACTTCTTTTGCCTTTGCAGGAAGAAATTTCCTTACAACAAAGAGCAGCAGCAGCCTCTTTGTTGGGGAAGCTTGTTGGCCAGCCAATGCACGGGCCTAGAGTTGTTATAACGTTAGCAAGGTTTCTTCCAGATGGCTTGGTTTCAGTTATTAGGGATGGACCTGGTGAGGCTGTCGTAGTTGCCCTTGAACAGACTACTGAGACCCCTGAACTTGTATGGACACCAGCAATGGCTACTTCATTGTCTGCACAGATTTCAACTATGGCATCAGATCTGTATCGCGAACAGATGAAAGGTCGTGTGGTTGATTGGGATGTACCTGAGCAGGCATCTGGGCAGCAAGAAATGAGAGATGAGCCACAGGTTGGCGGAATCTATGTTAGATTATTCCTAAAAGATCCCAAGTTTCCTCTCAGAAATCCCAAGAGATTCTTGGAAGGACTTCTAGATCAGTATTTGACATCTATTGCTGCCACACATTACGATACACTAGCTGTTGACCCTGAACTTCCTTTGCTTCTGTCTGCTGCGTTGGTTTCGTTATTACGAGTGCACCCTGCACTTGCAGATCATGTTGGATATCTTGGATATGTGCCCAAACTCGTGGCTGCTGTGGCTTATGAAGGAAGACGAGAAACAATGGCATCAGAGGAGGTAAACAATGGCAATTATGTGGACAAAACAGATGAATCTGATGACGGATCCACACAGCCCACTCAAACTGCCCAAGAACGTGTGCGCCTTAGTTGTTTACGTGTTCTACATCAACTGGCAGCTAGCACCACATGTGCTGAAGCTATGGCGGCGACTAGTGTAGGAACTCCTCAGGTCGTTCCCCTTCTAATGAAAGCAATAGGATGGCAAGGTGGAAGCATACTAGCTCTTGAGACATTGAAGCGTGTTGTGGTTGCTGGAAACCGAGCTAGGGATGCACTTGTTGCACAAGGACTTAAGGTTGGTCTTGTACAAGTACTTCTGGGCCTTCTTGATTGGAGAGCTGGAGGAAGGAATGGGCTTTGCTCTCAGATGAAGTGGAATGAATCTGAAGCATCTATTGGCAGGGTGCTCGCGATTGAGGTTTTGCATGCATTTGCAACGGAGGGGGCGCATTGTACTAAAGTGCGTGAGTTGTTAAATTCCTCAGATGTTTGGAGTGCTTATAAGGATCAGAAACATGATCTTTTCCTCCCTTCAAATGCTCAATCCGCTGCTGCCGGAATTGCTGGGCTAATTGAGAGTTCATCATCGAGACTTGCTTATGCCCTTACAGCTCCCTCACCACAACCTGCTCCATCCAGACCTCCTGCTTCAACTTCATCCGACTTAAATGGAAGACAGGATCACCTTTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006810 GO:0006887 GO:0006897 GO:0006898 GO:0006955 GO:0006996 GO:0007032 GO:0008150 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0012506 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0030141 GO:0030659 GO:0030667 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032879 GO:0032940 GO:0035577 GO:0036230 GO:0042119 GO:0042582 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043299 GO:0043312 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0060341 GO:0060627 GO:0065007 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099503 GO:1902954 GO:1903649 GO:2000641

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4339 DnaJ

Protein Analysis

2588

Amino Acids

282.77

Weight (kDa)

5.84

Isoelectric Point (pI)

45.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RME-8_N	PF19432	27 - 251	6.6e-23	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	299 - 746	2.4e-55	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	909 - 1008	1.9e-23	DNAJ protein RME-8 N-terminal
GYF_2	PF14237	1181 - 1229	9.3e-13	GYF domain 2
DnaJ	PF00226	1547 - 1587	3.7e-10	DnaJ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26890
fragaria_vesca	FvH4_1g04290 FvH4_1g04290 FvH4_1g04983
malus_domestica	MD02G1042600.v1.1 MD15G1181200.v1.1
prunus_persica	Prupe.7G234400_v2.0.a1 Prupe.7G234400_v2.0.a1
pyrus_communis	pycom02g03540 pycom15g16350
rosa_chinensis	RchiOBHm_Chr2g0089861
rosa_laevigata	RLG00000016062
rosa_multiflora	Rmu_co8299847.1_g000001 Rmu_sc0002384.1_g000010
rosa_roxburghii	Rroxscaffold_2G00151530
rosa_rugosa	Rorug02G0003700
rosa_samantha	Rh2AG049000 Rh2BG047900 Rh2CG050000 Rh2DG049100
rosa_wichuraiana	Rw2G004120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	5505, 6518, 7572
AarI	CACCTGC	2 cut(s)	532, 4255
AatII	GACGTC	1 cut(s)	198
Acc36I	ACCTGC	3 cut(s)	532, 4255, 7705
Acc65I	GGTACC	1 cut(s)	5331
AccB1I	GGYRCC	4 cut(s)	371, 405, 3345, 5331
AccB7I	CCANNNNNTGG	3 cut(s)	875, 1376, 6776
AccBSI	CCGCTC	2 cut(s)	3526, 4247
AccI	GTMKAC	5 cut(s)	331, 993, 1366, 2125, 6399
AccII	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
AccIII	TCCGGA	1 cut(s)	4398
AclI	AACGTT	1 cut(s)	6521
AcoI	YGGCCR	5 cut(s)	109, 4433, 4502, 6348, 6487
AcyI	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
AdeI	CACNNNGTG	1 cut(s)	1871
AflII	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
AflIII	ACRYGT	8 cut(s)	1564, 2219, 4447, 4985, 5275, 7144, 7165, 7196
AhdI	GACNNNNNGTC	2 cut(s)	3279, 6099
AhlI	ACTAGT	2 cut(s)	5197, 7220
AjiI	CACGTC	3 cut(s)	1080, 2114, 4986
AjnI	CCWGG	9 cut(s)	844, 1168, 1315, 2101, 2194, 4003, 4953, 5168, 6571
AjuI	GAANNNNNNNTTGG	6 cut(s)	4498, 4530, 5748, 5780, 7449, 7481
AleI	CACNNNNGTG	2 cut(s)	2702, 2769
AloI	GAACNNNNNNTCC	2 cut(s)	567, 599
Alw21I	GWGCWC	8 cut(s)	2660, 3085, 3231, 3562, 5090, 5871, 6966, 7479
Alw44I	GTGCAC	2 cut(s)	5867, 6962
AlwNI	CAGNNNCTG	6 cut(s)	1280, 1298, 1985, 4904, 5522, 5807
Ama87I	CYCGRG	2 cut(s)	1679, 5646
Aor13HI	TCCGGA	1 cut(s)	4398
ApaLI	GTGCAC	2 cut(s)	5867, 6962
ArsI	GACNNNNNNTTYG	2 cut(s)	6098, 6130
AseI	ATTAAT	1 cut(s)	3618
Asp700I	GAANNNNTTC	3 cut(s)	1618, 6324, 6852
Asp718I	GGTACC	1 cut(s)	5331
AspLEI	GCGC	9 cut(s)	374, 408, 816, 2055, 2063, 3060, 4755, 7152, 7519
AsuC2I	CCSGG	4 cut(s)	1680, 1681, 2800, 2880
AsuNHI	GCTAGC	1 cut(s)	7187
AvaI	CYCGRG	2 cut(s)	1679, 5646
AvaII	GGWCC	9 cut(s)	160, 424, 1676, 2324, 3281, 3569, 4172, 5693, 6569
AxyI	CCTNAGG	3 cut(s)	1404, 5940, 7236
BaeGI	GKGCMC	5 cut(s)	3043, 3296, 5871, 6966, 7008
BalI	TGGCCA	3 cut(s)	4435, 6350, 6489
BamHI	GGATCC	3 cut(s)	2261, 2708, 7112
BanI	GGYRCC	4 cut(s)	371, 405, 3345, 5331
BanII	GRGCYC	2 cut(s)	1023, 3231
BarI	GAAGNNNNNNTAC	2 cut(s)	7368, 7400
BauI	CACGAG	5 cut(s)	100, 825, 1083, 1323, 7013
BbsI	GAAGAC	3 cut(s)	3190, 7045, 7750
Bbv12I	GWGCWC	8 cut(s)	2660, 3085, 3231, 3562, 5090, 5871, 6966, 7479
BbvCI	CCTCAGC	3 cut(s)	1014, 2829, 4788
BceAI	ACGGC	6 cut(s)	35, 359, 2526, 4517, 4679, 5973
BciT130I	CCWGG	9 cut(s)	846, 1170, 1317, 2103, 2196, 4005, 4955, 5170, 6573
BciVI	GTATCC	1 cut(s)	1678
BclI	TGATCA	2 cut(s)	1989, 2761
BcnI	CCSGG	4 cut(s)	1680, 1681, 2800, 2880
BcuI	ACTAGT	2 cut(s)	5197, 7220
BfmI	CTRYAG	5 cut(s)	987, 4083, 4757, 5367, 5514
BfoI	RGCGCY	4 cut(s)	375, 409, 2064, 4756
BfrI	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
BfuAI	ACCTGC	3 cut(s)	532, 4255, 7705
BfuI	GTATCC	1 cut(s)	1678
BglI	GCCNNNNNGGC	2 cut(s)	2197, 5453
BglII	AGATCT	1 cut(s)	6682
BlpI	GCTNAGC	1 cut(s)	1329
BmcAI	AGTACT	2 cut(s)	1662, 7383
Bme18I	GGWCC	9 cut(s)	160, 424, 1676, 2324, 3281, 3569, 4172, 5693, 6569
BmeRI	GACNNNNNGTC	2 cut(s)	3279, 6099
BmeT110I	CYCGRG	2 cut(s)	1679, 5646
BmgBI	CACGTC	3 cut(s)	1080, 2114, 4986
BmtI	GCTAGC	1 cut(s)	7191
BpiI	GAAGAC	3 cut(s)	3190, 7045, 7750
BpmI	CTGGAG	7 cut(s)	1205, 1443, 3819, 3906, 4200, 4877, 7431
Bpu10I	CCTNAGC	7 cut(s)	1014, 2679, 2829, 3870, 4788, 5547, 6732
Bpu1102I	GCTNAGC	1 cut(s)	1329
BpuEI	CTTGAG	9 cut(s)	1865, 2319, 2320, 3038, 3512, 5249, 5800, 5951, 7316
BpuMI	CCSGG	4 cut(s)	1680, 1681, 2800, 2880
BsaAI	YACGTR	3 cut(s)	1532, 5444, 7166
BsaBI	GATNNNNATC	6 cut(s)	2154, 2250, 2421, 2766, 5724, 6687
BsaHI	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
BsaI	GGTCTC	2 cut(s)	477, 6605
BsaJI	CCNNGG	9 cut(s)	156, 845, 1169, 1679, 1680, 2195, 4551, 4793, 5169
BsaWI	WCCGGW	3 cut(s)	388, 2701, 4398
Bse1I	ACTGG	9 cut(s)	1101, 2442, 2650, 2911, 3065, 3662, 4346, 5556, 7185
Bse21I	CCTNAGG	3 cut(s)	1404, 5940, 7236
Bse3DI	GCAATG	6 cut(s)	654, 2048, 4200, 5215, 5776, 6645
Bse8I	GATNNNNATC	6 cut(s)	2154, 2250, 2421, 2766, 5724, 6687
BseAI	TCCGGA	1 cut(s)	4398
BseBI	CCWGG	9 cut(s)	846, 1170, 1317, 2103, 2196, 4005, 4955, 5170, 6573
BseDI	CCNNGG	9 cut(s)	156, 845, 1169, 1679, 1680, 2195, 4551, 4793, 5169
BseJI	GATNNNNATC	6 cut(s)	2154, 2250, 2421, 2766, 5724, 6687
BseMI	GCAATG	6 cut(s)	654, 2048, 4200, 5215, 5776, 6645
BseNI	ACTGG	9 cut(s)	1101, 2442, 2650, 2911, 3065, 3662, 4346, 5556, 7185
BseRI	GAGGAG	9 cut(s)	10, 74, 1031, 3222, 4502, 4622, 5629, 7076, 7224
BseSI	GKGCMC	5 cut(s)	3043, 3296, 5871, 6966, 7008
BseYI	CCCAGC	2 cut(s)	3636, 7634
BsgI	GTGCAG	7 cut(s)	2334, 2418, 2793, 3759, 5988, 6642, 6954
Bsh1236I	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
BshNI	GGYRCC	4 cut(s)	371, 405, 3345, 5331
BsiHKAI	GWGCWC	8 cut(s)	2660, 3085, 3231, 3562, 5090, 5871, 6966, 7479
BsiHKCI	CYCGRG	2 cut(s)	1679, 5646
BslFI	GGGAC	6 cut(s)	410, 1041, 1450, 1662, 3267, 3695
BsmBI	CGTCTC	2 cut(s)	1086, 2278
BsmFI	GGGAC	6 cut(s)	410, 1041, 1450, 1662, 3267, 3695
BsmI	GAATGC	4 cut(s)	418, 2341, 2586, 4588
Bso31I	GGTCTC	2 cut(s)	477, 6605
BsoBI	CYCGRG	2 cut(s)	1679, 5646
Bsp13I	TCCGGA	1 cut(s)	4398
Bsp1407I	TGTACA	4 cut(s)	4735, 4833, 5413, 7375
Bsp1720I	GCTNAGC	1 cut(s)	1329
Bsp68I	TCGCGA	2 cut(s)	6693, 7481
BspEI	TCCGGA	1 cut(s)	4398
BspFNI	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
BspHI	TCATGA	2 cut(s)	2590, 3454
BspMAI	CTGCAG	5 cut(s)	991, 4087, 4761, 5371, 5518
BspMI	ACCTGC	3 cut(s)	532, 4255, 7705
BspOI	GCTAGC	1 cut(s)	7191
BspQI	GCTCTTC	1 cut(s)	5007
BspT107I	GGYRCC	4 cut(s)	371, 405, 3345, 5331
BspTI	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
BspTNI	GGTCTC	2 cut(s)	477, 6605
BsrBI	CCGCTC	2 cut(s)	3526, 4247
BsrDI	GCAATG	6 cut(s)	654, 2048, 4200, 5215, 5776, 6645
BsrGI	TGTACA	4 cut(s)	4735, 4833, 5413, 7375
BsrI	ACTGG	9 cut(s)	1101, 2442, 2650, 2911, 3065, 3662, 4346, 5556, 7185
BssECI	CCNNGG	9 cut(s)	156, 845, 1169, 1679, 1680, 2195, 4551, 4793, 5169
BssNAI	GTATAC	3 cut(s)	332, 994, 1367
BssNI	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
BssSI	CACGAG	5 cut(s)	100, 825, 1083, 1323, 7013
BssT1I	CCWWGG	3 cut(s)	156, 4551, 4793
Bst1107I	GTATAC	3 cut(s)	332, 994, 1367
Bst2BI	CACGAG	5 cut(s)	100, 825, 1083, 1323, 7013
Bst2UI	CCWGG	9 cut(s)	846, 1170, 1317, 2103, 2196, 4005, 4955, 5170, 6573
Bst6I	CTCTTC	5 cut(s)	2472, 3656, 4040, 4099, 5007
BstACI	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
BstAFI	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
BstAPI	GCANNNNNTGC	6 cut(s)	1280, 2080, 4270, 4777, 5522, 7349
BstAUI	TGTACA	4 cut(s)	4735, 4833, 5413, 7375
BstBAI	YACGTR	3 cut(s)	1532, 5444, 7166
BstFNI	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
BstH2I	RGCGCY	4 cut(s)	375, 409, 2064, 4756
BstHHI	GCGC	9 cut(s)	374, 408, 816, 2055, 2063, 3060, 4755, 7152, 7519
BstNI	CCWGG	9 cut(s)	846, 1170, 1317, 2103, 2196, 4005, 4955, 5170, 6573
BstSFI	CTRYAG	5 cut(s)	987, 4083, 4757, 5367, 5514
BstSLI	GKGCMC	5 cut(s)	3043, 3296, 5871, 6966, 7008
BstSNI	TACGTA	1 cut(s)	1532
BstUI	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
BstV2I	GAAGAC	3 cut(s)	3190, 7045, 7750
BstXI	CCANNNNNNTGG	2 cut(s)	1919, 7015
BstZ17I	GTATAC	3 cut(s)	332, 994, 1367
Bsu36I	CCTNAGG	3 cut(s)	1404, 5940, 7236
BsuI	GTATCC	1 cut(s)	1678
BtgZI	GCGATG	2 cut(s)	2070, 3257
BtrI	CACGTC	3 cut(s)	1080, 2114, 4986
BtsI	GCAGTG	7 cut(s)	2604, 3034, 3294, 4123, 4309, 5088, 5364
BtuMI	TCGCGA	2 cut(s)	6693, 7481
BveI	ACCTGC	3 cut(s)	532, 4255, 7705
CaiI	CAGNNNCTG	6 cut(s)	1280, 1298, 1985, 4904, 5522, 5807
CciI	TCATGA	2 cut(s)	2590, 3454
CfoI	GCGC	9 cut(s)	374, 408, 816, 2055, 2063, 3060, 4755, 7152, 7519
Cfr9I	CCCGGG	1 cut(s)	1679
CseI	GACGC	6 cut(s)	954, 1919, 2295, 2550, 4848, 6102
CsiI	ACCWGGT	1 cut(s)	6571
CspCI	CAANNNNNGTGG	2 cut(s)	190, 225
DinI	GGCGCC	2 cut(s)	373, 407
DraI	TTTAAA	3 cut(s)	474, 837, 4867
DraIII	CACNNNGTG	1 cut(s)	1871
DriI	GACNNNNNGTC	2 cut(s)	3279, 6099
EaeI	YGGCCR	5 cut(s)	109, 4433, 4502, 6348, 6487
Eam1104I	CTCTTC	5 cut(s)	2472, 3656, 4040, 4099, 5007
Eam1105I	GACNNNNNGTC	2 cut(s)	3279, 6099
EarI	CTCTTC	5 cut(s)	2472, 3656, 4040, 4099, 5007
EciI	GGCGGA	3 cut(s)	205, 579, 6795
Ecl136II	GAGCTC	1 cut(s)	3229
Eco105I	TACGTA	1 cut(s)	1532
Eco130I	CCWWGG	3 cut(s)	156, 4551, 4793
Eco147I	AGGCCT	2 cut(s)	1650, 2664
Eco24I	GRGCYC	2 cut(s)	1023, 3231
Eco31I	GGTCTC	2 cut(s)	477, 6605
Eco32I	GATATC	3 cut(s)	2963, 3676, 6992
Eco47I	GGWCC	9 cut(s)	160, 424, 1676, 2324, 3281, 3569, 4172, 5693, 6569
Eco53kI	GAGCTC	1 cut(s)	3229
Eco81I	CCTNAGG	3 cut(s)	1404, 5940, 7236
Eco88I	CYCGRG	2 cut(s)	1679, 5646
EcoICRI	GAGCTC	1 cut(s)	3229
EcoO109I	RGGNCCY	4 cut(s)	424, 3281, 4784, 5693
EcoRI	GAATTC	3 cut(s)	643, 2738, 6073
EcoRII	CCWGG	9 cut(s)	844, 1168, 1315, 2101, 2194, 4003, 4953, 5168, 6571
EcoRV	GATATC	3 cut(s)	2963, 3676, 6992
EcoT14I	CCWWGG	3 cut(s)	156, 4551, 4793
EcoT22I	ATGCAT	4 cut(s)	1260, 1363, 5436, 7501
EcoT38I	GRGCYC	2 cut(s)	1023, 3231
EgeI	GGCGCC	2 cut(s)	373, 407
EheI	GGCGCC	2 cut(s)	373, 407
ErhI	CCWWGG	3 cut(s)	156, 4551, 4793
Esp3I	CGTCTC	2 cut(s)	1086, 2278
FaqI	GGGAC	6 cut(s)	410, 1041, 1450, 1662, 3267, 3695
FauI	CCCGC	5 cut(s)	55, 86, 172, 1187, 3663
FauNDI	CATATG	2 cut(s)	5707, 6232
FbaI	TGATCA	2 cut(s)	1989, 2761
FblI	GTMKAC	5 cut(s)	331, 993, 1366, 2125, 6399
FriOI	GRGCYC	2 cut(s)	1023, 3231
GlaI	GCGC	9 cut(s)	373, 407, 815, 2054, 2062, 3059, 4754, 7151, 7518
GsaI	CCCAGC	2 cut(s)	3640, 7638
GsuI	CTGGAG	7 cut(s)	1205, 1443, 3819, 3906, 4200, 4877, 7431
HaeII	RGCGCY	4 cut(s)	375, 409, 2064, 4756
HgaI	GACGC	6 cut(s)	954, 1919, 2295, 2550, 4848, 6102
HhaI	GCGC	9 cut(s)	374, 408, 816, 2055, 2063, 3060, 4755, 7152, 7519
Hin1I	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
Hin6I	GCGC	9 cut(s)	372, 406, 814, 2053, 2061, 3058, 4753, 7150, 7517
HinP1I	GCGC	9 cut(s)	372, 406, 814, 2053, 2061, 3058, 4753, 7150, 7517
HincII	GTYRAC	6 cut(s)	911, 2126, 2580, 4494, 5541, 6910
HindII	GTYRAC	6 cut(s)	911, 2126, 2580, 4494, 5541, 6910
HindIII	AAGCTT	7 cut(s)	615, 2968, 4527, 4655, 4743, 5549, 6479
Hsp92I	GRCGYC	6 cut(s)	195, 372, 406, 946, 2561, 6113
HspAI	GCGC	9 cut(s)	372, 406, 814, 2053, 2061, 3058, 4753, 7150, 7517
KasI	GGCGCC	2 cut(s)	371, 405
KflI	GGGWCCC	1 cut(s)	424
Kpn2I	TCCGGA	1 cut(s)	4398
KpnI	GGTACC	1 cut(s)	5335
Ksp22I	TGATCA	2 cut(s)	1989, 2761
LguI	GCTCTTC	1 cut(s)	5007
MabI	ACCWGGT	1 cut(s)	6571
MbiI	CCGCTC	2 cut(s)	3526, 4247
MfeI	CAATTG	6 cut(s)	1035, 2001, 2019, 2237, 3249, 4437
MlsI	TGGCCA	3 cut(s)	4435, 6350, 6489
MluNI	TGGCCA	3 cut(s)	4435, 6350, 6489
Mly113I	GGCGCC	2 cut(s)	372, 406
MmeI	TCCRAC	8 cut(s)	273, 748, 1570, 1809, 3547, 3633, 6967, 7755
Mox20I	TGGCCA	3 cut(s)	4435, 6350, 6489
Mph1103I	ATGCAT	4 cut(s)	1260, 1363, 5436, 7501
MroI	TCCGGA	1 cut(s)	4398
MroXI	GAANNNNTTC	3 cut(s)	1618, 6324, 6852
MscI	TGGCCA	3 cut(s)	4435, 6350, 6489
MslI	CAYNNNNRTG	7 cut(s)	1068, 1730, 1767, 2702, 2769, 4071, 4281
Msp20I	TGGCCA	3 cut(s)	4435, 6350, 6489
MspA1I	CMGCKG	8 cut(s)	1225, 1280, 2567, 4178, 4190, 5519, 6377, 7619
MspCI	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
MunI	CAATTG	6 cut(s)	1035, 2001, 2019, 2237, 3249, 4437
Mva1269I	GAATGC	4 cut(s)	418, 2341, 2586, 4588
MvaI	CCWGG	9 cut(s)	846, 1170, 1317, 2103, 2196, 4005, 4955, 5170, 6573
MvnI	CGCG	5 cut(s)	237, 2055, 3060, 6693, 7481
NarI	GGCGCC	2 cut(s)	372, 406
NciI	CCSGG	4 cut(s)	1680, 1681, 2800, 2880
NdeI	CATATG	2 cut(s)	5707, 6232
NheI	GCTAGC	1 cut(s)	7187
NmeAIII	GCCGAG	2 cut(s)	442, 5109
NruI	TCGCGA	2 cut(s)	6693, 7481
NsiI	ATGCAT	4 cut(s)	1260, 1363, 5436, 7501
OliI	CACNNNNGTG	2 cut(s)	2702, 2769
PaeI	GCATGC	5 cut(s)	1527, 1724, 2087, 5438, 7499
PagI	TCATGA	2 cut(s)	2590, 3454
PaqCI	CACCTGC	2 cut(s)	532, 4255
PceI	AGGCCT	2 cut(s)	1650, 2664
PciI	ACATGT	4 cut(s)	1564, 4447, 5275, 7196
PciSI	GCTCTTC	1 cut(s)	5007
PcsI	WCGNNNNNNNCGW	1 cut(s)	201
PctI	GAATGC	4 cut(s)	418, 2341, 2586, 4588
PdmI	GAANNNNTTC	3 cut(s)	1618, 6324, 6852
PflMI	CCANNNNNTGG	3 cut(s)	875, 1376, 6776
PfoI	TCCNGGA	3 cut(s)	1315, 2878, 4003
PluTI	GGCGCC	2 cut(s)	375, 409
Ppu21I	YACGTR	3 cut(s)	1532, 5444, 7166
PpuMI	RGGWCCY	3 cut(s)	424, 3281, 5693
PscI	ACATGT	4 cut(s)	1564, 4447, 5275, 7196
PshBI	ATTAAT	1 cut(s)	3618
PsiI	TTATAA	3 cut(s)	5505, 6518, 7572
Psp124BI	GAGCTC	1 cut(s)	3231
Psp1406I	AACGTT	1 cut(s)	6521
Psp5II	RGGWCCY	3 cut(s)	424, 3281, 5693
Psp6I	CCWGG	9 cut(s)	844, 1168, 1315, 2101, 2194, 4003, 4953, 5168, 6571
PspFI	CCCAGC	2 cut(s)	3636, 7634
PspGI	CCWGG	9 cut(s)	844, 1168, 1315, 2101, 2194, 4003, 4953, 5168, 6571
PspPPI	RGGWCCY	3 cut(s)	424, 3281, 5693
PstI	CTGCAG	5 cut(s)	991, 4087, 4761, 5371, 5518
PstNI	CAGNNNCTG	6 cut(s)	1280, 1298, 1985, 4904, 5522, 5807
PvuII	CAGCTG	6 cut(s)	1280, 2567, 4178, 4190, 5519, 6377
RruI	TCGCGA	2 cut(s)	6693, 7481
RseI	CAYNNNNRTG	7 cut(s)	1068, 1730, 1767, 2702, 2769, 4071, 4281
SacI	GAGCTC	1 cut(s)	3231
SalI	GTCGAC	1 cut(s)	2124
SapI	GCTCTTC	1 cut(s)	5007
SbfI	CCTGCAGG	1 cut(s)	4761
ScaI	AGTACT	2 cut(s)	1662, 7383
SdaI	CCTGCAGG	1 cut(s)	4761
SexAI	ACCWGGT	1 cut(s)	6571
SfcI	CTRYAG	5 cut(s)	987, 4083, 4757, 5367, 5514
SfoI	GGCGCC	2 cut(s)	373, 407
SinI	GGWCC	9 cut(s)	160, 424, 1676, 2324, 3281, 3569, 4172, 5693, 6569
SmaI	CCCGGG	1 cut(s)	1681
SmiI	ATTTAAAT	1 cut(s)	4867
SmiMI	CAYNNNNRTG	7 cut(s)	1068, 1730, 1767, 2702, 2769, 4071, 4281
SnaBI	TACGTA	1 cut(s)	1532
SpeI	ACTAGT	2 cut(s)	5197, 7220
SphI	GCATGC	5 cut(s)	1527, 1724, 2087, 5438, 7499
Sse8387I	CCTGCAGG	1 cut(s)	4761
SseBI	AGGCCT	2 cut(s)	1650, 2664
SspDI	GGCGCC	2 cut(s)	371, 405
SspI	AATATT	2 cut(s)	4539, 5189
SstI	GAGCTC	1 cut(s)	3231
StuI	AGGCCT	2 cut(s)	1650, 2664
StyI	CCWWGG	3 cut(s)	156, 4551, 4793
SwaI	ATTTAAAT	1 cut(s)	4867
TaqII	GACCGA	1 cut(s)	353
TatI	WGTACW	9 cut(s)	738, 1660, 4735, 4833, 5413, 5492, 7375, 7381, 7523
TauI	GCSGC	6 cut(s)	114, 237, 1228, 1499, 4964, 7217
TspMI	CCCGGG	1 cut(s)	1679
Van91I	CCANNNNNTGG	3 cut(s)	875, 1376, 6776
Vha464I	CTTAAG	4 cut(s)	1793, 3923, 6080, 7361
VneI	GTGCAC	2 cut(s)	5867, 6962
VpaK11BI	GGWCC	9 cut(s)	160, 424, 1676, 2324, 3281, 3569, 4172, 5693, 6569
VspI	ATTAAT	1 cut(s)	3618
XbaI	TCTAGA	2 cut(s)	2180, 6856
XcmI	CCANNNNNNNNNTGG	6 cut(s)	154, 1211, 2931, 4224, 5628, 6841
XmaI	CCCGGG	1 cut(s)	1679
XmiI	GTMKAC	5 cut(s)	331, 993, 1366, 2125, 6399
XmnI	GAANNNNTTC	3 cut(s)	1618, 6324, 6852
ZraI	GACGTC	1 cut(s)	196
ZrmI	AGTACT	2 cut(s)	1662, 7383
Zsp2I	ATGCAT	4 cut(s)	1260, 1363, 5436, 7501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.