Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0010166

Physical Location & Seq

Genomic Coordinates Reverse (-)

3600651 .. 3614917

Gene Structure 14267 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016062

Sequence Viewer

CDS Protein Promoter (2k)

Length: 8079 bp

ATGAATCACCAGCCACGTCATGGAAGAGTAGTCAGCGGCTCTCTCTCCCGTGTGACGGGTACCTCTTATAACGCAACCGGATGGTGCTCCTCTTTGACAAGTGGCACCGCCCGTAAATATCCGTCAGAGCTGGGGGCGAATCACTCTCGTTCTCGGGCTCCGGCCGCGGCTCCTCCTTCTCCCCTTCCTCCCTGGCTCTTCTTCCTCCGCCGCAACAATGCGCCTCGCCCTCACACCCTCCACTACCTCCCGCACGTAGATTCCGTCTCCCGCCATCACGTCTCCGATCACCGCCCTCCTCCTCCGCCGCCGACTCAGCCACGCATGGACCAACCTAACACCTCCACTTCTTCTTCTTCTTCTTCTTCCCGTTCTGCCCCTGTCGAAGAGCCGGAGTACCTCGCGCGCTACTTAGTCGTCAAGCACTCGTGGCGCGGCCGCTACAAGCGGATTCTCTGCCTCTCGAGCGCCTCCATCGTCACCTTGGACCCGTCCACCCTCGCCGTCACGAACTCGTACGTCGTCGCGTCGGATTTCGACACCGCCGCGCCGATCATCGGCCGCGACGAGAGCTCCAGTGAGTTCAATTTGAGTGTTCGGACTGACGGCAGGGGGAAGTTCAAGTCGGTGAAGTTCTCGTCCAGGTATAGGGCGAGCATACTGACCGAGCTGCAGAGGATTAGGGGCAATCGGCTGGGGGTAGTAGCGGAATTTCCAGTGCTTCATCTCCGGCGACGGACTGGCGAGTGGGTTCCTTTTAAACTAAAAGTTACTTATGTTGGGGTTGAACTTATTGATTTAAAATGTGGAGACCTCCGCTGGTGCTTGGATTTTAGAGATTTTCATTCCCCTGCCATTATTTCCCTTTCCGATGCTTATGGGAAGAAAGGCATTGAAGGAGGTTTTATTCTTTGTCCTTCATATGGAAGAAAATCGAAAGCTTTTCAAGCTGCTTCTGGAACAACAAATTCTGTTATCATTGCTAATTTGACTAAAACAGCAAAATCTATGGTCGGGGTGTCACTAAATGTGGAAACTTCTCAGTCTCTCACTATAGCAGAGTATATAAAGCGAAGAGCAAAAGAAGCAGTTGGAGCAGATGAAACTCCATGTGGAGGTTGGTCTGTGACAAGATTGCGCTCTGCTGCTCGTGGAACTCTAAATGTTCCAGGATTGAATTTGAGCGTTGGCCCAAAAGGTGGACTTGGGGAGCATGGTGATGCTGTATCTCGTCAATTGATTCTTACAAAAGTTTCACTTGTTGAAAGACGCCCAGAGAACTATGAAGCTGTCATCGTTCGACCTTTATCTGCAGTAAATGCCCTTGTTCGATTTACTGAGGAGCCTCAGATGTTTGCAATTGAGTTCAATGATGGATGCCCCATCCATGTATATGCAAGCACCTCTCGTGATAGCTTACTTGCGGCAGTTCGTGATCTGTTGCAAACAGAAGGTCAATGTGCTATGGCTGTGTTACCAAGACTGACGATGCCTGGCCATCGCATTGATCCACCTTGCGGTAGAGTTAATTTACAATTTGGACTACAACGGCCTATTGCAGATATGGAGAGTGCATCCATGCATTTGAAGCACTTAGCAGCAGCTGCCAAAGATGCAGTTTCTGAAGGTGGTTCCATTCCGGGATCAAGAGCTAAGCTCTGGCGCAGAATAAGAGAGTTCAATGCATGCATACCATATACTGGAGTCCCTCCTAACATAGAAGTACCTGAGGTGACTTTGATGGCTTTAATAACAATGCTTCCAGCTACGCCAAATCTTCCTCCAGAGTCCCCTCCCTTGCCACCCCCTTCACCTAAAGCAGCTGCAACAGTCATGGGCTTTATTGCATGTTTACGTAGATTGTTGGCTTCAAGAACTGCAGCTTCACATGTCATGTCTTTTCCTGCTGCTGTTGGAAGAATAATGGGCTTACTCAGAAATGGATCAGAGGGTGTAGCCGCTGAAGCAGCAGGCCTTATTGCAGTTCTTATTGGTGGTGGTCCTGGGGATACAAATATATTGACAGATTCTAAAGGTGAGCATTATGCCACAATCATGCACACAAAGTCAGTGTTGTTTGCGCAACAAGGTTATGTCATTATACTTGCCAATAGACTGAAGCCCATGTCTGTATCACCACTATTATCAATGGCCGTTGTTGAGGTTCTTGAGGCAATGATATGTGATCCACATGGTGAGACAACACAGTACCCAGTGTTTGTTGAACTATTACGCCAAGTAGCTGGTTTGAAACGTCGTTTGTTTGCATTGTTTGGACATCCTGCTGAAAGTGTTCGGGAAACAGTGGCTGTGATTATGCGTACAATTGCTGAAGAAGATGCAATTGCAGCAGAGTCCATGCGCGATGCTGCATTGCGTGATGGTGCTTTGTTGAGGCATTTATTGCATGCGTTCTTCCTTCCGGCTGGTGAGCGACGTGAGGTTAGTCGACAGCTTGTTGCTCTTTGGGCAGATTCGTATCAACCCGCTCTGGATTTATTGTCTAGAGTTCTGCCTCCTGGACTTGTTGCTTATTTGCACACAAAATCTGATGGAGTTCTATCAGAAGAGTCTAATCAAGAAGTTTCCTTGACCAGTAGAAGACAGAGACGCTTACTTCAACAGAGGAGAGGCCGTACCGGAAGAGGAGCAACATCCCAGGAGCATTCGTTGCCTTCTGTAAACAATTATGATGTGAATGACCCCATGACACAGACAAGTGGTGATGTCTCGAAAGTGTCAAATAACTATCAAAGATCTGCAATGGATCCAAATTCTGGACAGGCTCCGACCATTCAATCTTCTGCTGCTCAAACTGGTGAAAACTTGATTGGTGAAGTATCCTCCACTGGGGCTCCACAAAGCAATAATACATCTATTGTGGCTTCAGCTGATGCTCCATCAACAGGTGTTCATGCTTCATTGGCAGCAAATACTGCAATCTCAACAGATTCTGACTCCAATGTTGCTGGTTTCCATAATATGGGTCTCCCTGCCCCTGCTCAGGTTGTTGTGGAAAACACTCCTGTGGGATCCGGTAGGCTTCTATGTAATTGGCCTGAATTCTGGCGAGCATTTAGCCTTGATCATAATCGTGCAGATTTGATCTGGAATGAGCGTACCAGGCAAGAGTTAAGGGAGGCCTTGCAGGCTGAGGTTCATAAGTTAGATGTTGAGAAGGAGCGTACAGAAGATATTGTTCCAAGAGATTCTACAGTAGAGATGACTGGGCAAGATAGTGTGCCGCAGATATCTTGGAACTACTCTGAGTTCTCTGTTGGATATCCTAGTTTGTCTAAAGAAGTTTGTGTGGGTCAGTATTATCTGCGCTTGCTGCTTGAGAGTGGCAGTGGTGGCAGGGCACAAGAATTTCCACTGCGCGATCCTGTTGCCTTCTTTAGAGCACTCTATCATCGTTTCTTATGTGATGCAGACATAGGGCTTACAGTAGATGGTGCTGTTCCGGATGAAATGGGCGCATCTGATGATTGGTGCGACATGGGAAGACTAGATGGTTTTGGAGGTGGAGGGGGATTTTCAGTTAGGGAGCTTTGTGCAAGAGCAATGACAATTGTATATGAACAGCATTACAAAACAGTAGGTCCCTTTGAGGGTACTGCTCACATTACAGTTCTGTTGGACAGGACAGATGATAGGGCTTTGAGGCACCGCCTTCTGTTGCTTTTGAAGGCTTTAATGAAGGTGTTATCAAATGTGGAGGCGTGCGTCTTGGTTGGAGGATGTGTGCTAGGGGTTGACATGCTGACAGCTGTTCATGAAGCTTCTGAAAGGACGGCTATTCCTTTGCAATCCAACTTGATTGCTGCTACTGCCTTCATGGAACCGCTCAAGGAATGGATGTTTTTTGACAAGGAAGGAGCACAAGTTGGACCTGTTGAAAAGGATGCAATAAGAAGATTCTGGTCCAAGAAGGCAATTGATTGGACAACAAAGTGCTGGGCTTCTGGGATGCTAGACTGGAAGAGGTTACGTGATATTCGTGAACTTCGTTGGGCGCTAGCTGTTCGGGTTCCTGTTCTCACGCCAGCTCAGGTTGGGGAGGCGGCATTGTCCATATTGCATAGCATGGTATCTGCACATTCTGATTTAGATGATGCTGGAGAAATAGTCACCCCAACTCCTAGAGTAAAAAGGATATTGTCAAGTCCGCGTTGTCTTCCGCATATTGCTCAGGCCATGCTTTCTGGAGAACCAAGTATTGTAGAGAGTGCTGCTGCATTACTGAAGGCTGTTGTTACCAGAAATCCGAAGGCCATGATTCGTCTATACAGCACAGGCACATTTTATTTTTCCCTGGCATATCCGGGATCAAATCTCCTTTCAATTGCCCAACTCTTTTCAGTGACTCATGTTCACCAAGCATTTCATGGTGGCGAAGATGCTGCAGTTTCCTCTTCCTTACCTCTGGCGAAACGCAGTGTATTGGGTGGACTTCTTCCTGAATCTCTGCTGTATGTATTAGAGCGTAGTGGTCCAGGTGCATTTGCAGCTGCAATGGTATCTGATTCTGATACTCCAGAGATTATATGGACTCACAAGATGCGAGCAGATAATCTGATACGTCAGGTTTTGCAGCATCTTGGTGACTTTCCCCAGAAATTGTCACAGCACTGCCATTGTCTATATGAGTATGCTCCTATGCCGCCAGTGACATACCCAGAGCTAAGAGATGAAATGTGGTGTCACCGTTATTACCTCCGCAACTTATGTGATGAGATTAGATTTCCAAATTGGCCAATTGTTGAACATGTTGAGTTTCTACAGTCATTACTGGTAATGTGGCGTGAGGAACTTACAAGAAGACCGATGGATCTTTCTGAGGAAGAAGCTTGCAAAATATTAGAGATTTCCTTGGAAGATGTGTCAAGTGATGATGCCAATATGAAACATTCTGTTGAGATGGGTGAGGATACGTCTAGCATATCCAAGCAGATTGAGAATATTGATGAAGAAAAGCTCAAGCGACAATATAGGAAACTTGCCATGAGATACCATCCAGACAAAAATCCTGAAGGAAGGGATAAGTTTCTTGCTGTACAGAAAGCTTATGAGCGCCTGCAGGCCACTATGCAAGGCTTGCAAGGCCCTCAGGCTTGGCGGTTGTTGCTTTTATTGAAAGGGCAATGCATCTTGTACAGAAGATATGGAGATATATTGGAACCGTTTAAATATGCTGGTTATCCGATGTTGCTCAGTGCAGTTACTGTGGACAAGGATGATAACAATTTTCTTTCCTTAGAGAGAGCACCTCTCCTTGTTGCAGCATCAGAGCTTATTTGGCTTACGTGTGCATCTTCTTCATTGAATGGTGAAGAGCTTGTGAGGGATGGTGGCATACAACTTCTTGGAAATCTTCTTTCCCGTTGCATGTGTGTAGTTCAGCCAACTACTCCTGCAAGTGAACCATCTGCCATAATTGTTACAAATGTTATGCGCACCTTCTGTGTTTTGAGTCAATTTGAGAGTGCCTGGGCAGAGATACTTGAATATTCTGGACTAGTGGATGATATTGTGCACTGCACTGAGCTGGAGCTTGTACCTGCTGCTGTAGATGCTGCCCTCCAGACTATTGCCCATGTTTCTGTGTCTACTCAATTGCAAGATGCCTTGCTTAAGGCTGGTGTCTTATGGTACCTTTTGCCCGTGCTGCTTCAGTATGACTCTACTGCAGAGGAATCTGATACAACAGAATCACATGGTGTTGGTGCTAGCGTACAGATTGCCAAAAATATGCATGCTATACGTGCGTCACAGGCGCTATCAAGGCTTAGTGGTTTGTGTAGTAATGAGAGTTCAACACCTTATAATCAGACTGCAGCTGATGCCCTCAGAGCTTTGTTGACTCCAAAACTTGCCAGTATGTTAAAAGATCAAGCTCCTAAAGACCTACTGTCAAAATTAAACAATAACTTGGAGTCTCCAGAGATTATCTGGAACTCTTCCACCCGAGCAGAACTACTGAAGTTTGTGGATCAGCAACGTGCAAGTCAGGGTCCAGACGGCTCATATGACCTGAAAGATTCGCATATGTTTGTGTACAAGGCACTGTCAAAAGAACTTTATGTTGGCAATGTTTACTTGAGGGTGTATAACGATCAGCCCGATTTTGATATTAGTGAACCAGAGGCTTTCTGTGTTGCTTTAATTGATTTTATATCATATCTAGTACACAATCAATGTTCTCAAGATTCAGAAGTTCACGATGAGCCAAAGCAGGATGGCTCATCCCTTGAAACATCTGAGCATCCTAATGATACGGCTATTAGATCAGTGGATGAACTTAGTGTTCCAGTTGAAGATTTGACAGTATCCAACGGCAAAGTGGCAGACAAAGAAGAATCTAAAGTGGTCAAAAACCTTAAGTTTGCATTGAATTCACTCAAGAACATACTGACAAGCAGTCCAAATTTGGCGTCAATTTTTTCTACTAAAGACAAGCTTTTGCCTCTTTTTGAATGCTTCTCTGTGCCTGTTGCACCAGAGAGTAACATACCTCAACTTTGCCTGAGTGTGTTGTCACTCTTGACAACATATGCTCCCTGCTTGGAGGCTATGGTTGCAGATGGATCTAGCCTTCTCCTTTTATTGCAAATGCTTCACTCAGCCCCAAGTTGTCGTGAGGGGGTTCTTCATGTTCTATATGCTCTGGCAAGCACACCAGAGCTTGCTTGGGCAGCTGCCAAGCATGGAGGAGTGGTCTACATTCTTGAGCTTCTCTTGCCTTTGCAGGAAGAAATTTCCTTGCAGCAAAGAGCAGCTGCAGCCTCTTTGTTGGGGAAGCTTGTTGGGCAGCCAATGCATGGGCCAAGAGTTGCTATAACGCTGGCAAGGTTTCTTCCTGATGGCTTGGTATCAGTTATTAGGGATGGACCTGGTGAAGCTGTTGTAGTTGCCCTTGAACAGACTACTGAGACTCCAGAACTTGTATGGACACCAGCGATGGCTACTTCTCTGTCTGCACAAATCGCGACGATGGCAGCAGATCTATACCAAGAACAAATGAAAGGCCGTGTTGTTGACTGGGACGTACCTGAGCAGGCATCTGGGCAGCAAGAAATGAGAGATGAGCCACAGGTTGGTGGAATATATATTAGGTTATTCTTAAAAGATCCTAAATTCCCTCTCAGAAATCCAAAGAGGTTCTTGGAAGGACTGCTAGATCAATATTTGACATCCATTGCCGCCACACATTATGAAACACAAGCTGTCGACCCTGAACTTCCTTTGCTCCTGTCTGCTGCTTTGGTTTCATTATTGCGAGTACATCCTGCCCTTGCAGATCATGTTGGATACCTTGGATATGTGCCCAAACTAGTGGCTGCTGTGGCTTATGAAGGAAGACGAGAAACAATGGCATCGGGGGAGGTGAACAATGGAAACTATGTGGACAGAGCAGAGGAGTCTGATGATACACAGCCTGCCCAAACTCCCCAAGAACGTGTGCGCCTCAGTTGTCTGCGTGTTCTACATCAATTGGCAGCTAGCACCACATGTGCTGAAGCTATGGCAGCAACTAGTGTAGGAACACCTCAGGTCGTTCCTCTTCTAATGAAAGCTATAGGATGGCAAGGTGGAAGCATACTAGCTCTGGAGACCCTAAAGCGTGTTGTTGTTGCTGGAAACCGAGCTAGGGATGCACTCGTTGCACAAGGACTTAAGGTTGGTCTTGTTGAAGTACTTCTTGGCCTTCTTGATTGGAGAGCTGGGGGAAGGAATGGACTTTGCTCTCAGATGAAGTGGAATGAATCTGAAGCATCTATTGGCAGAGTGCTAGCAATTGAGGTTTTGCATGCATTCGCGACGGAGGGGGCCCACTGTACTAAAGTGCGGGACCTATTGAATTCCTCAGATGTTTGGAGTGCTTATAAAGATCAGAAACATGATCTTTTCCTTCCTTCAAGTGCTCAATCTGCTGCCGCTGGAATTGCTGGGCTAATAGAAAATTCATCATCAAGACTCACTTATGCCATTACAGCTCCTCCACCACAACCCTCTACATCGAGACCTCCAGCGTCAACTACATTTGAATCAAATGGAAGATATGATCAGCTATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006810 GO:0006887 GO:0006897 GO:0006898 GO:0006955 GO:0006996 GO:0007032 GO:0008150 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0012506 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0030141 GO:0030659 GO:0030667 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032879 GO:0032940 GO:0035577 GO:0036230 GO:0042119 GO:0042582 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043299 GO:0043312 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0060341 GO:0060627 GO:0065007 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099503 GO:1902954 GO:1903649 GO:2000641

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4339 DnaJ

Protein Analysis

2693

Amino Acids

294.41

Weight (kDa)

6.08

Isoelectric Point (pI)

47.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RME-8_N	PF19432	136 - 359	1.9e-22	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	405 - 859	6.6e-56	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	1017 - 1115	1.4e-24	DNAJ protein RME-8 N-terminal
GYF_2	PF14237	1288 - 1336	1.8e-12	GYF domain 2
DnaJ	PF00226	1654 - 1694	4.3e-10	DnaJ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26890
fragaria_vesca	FvH4_1g04290 FvH4_1g04290 FvH4_1g04983
malus_domestica	MD02G1042600.v1.1 MD15G1181200.v1.1
prunus_persica	Prupe.7G234400_v2.0.a1 Prupe.7G234400_v2.0.a1
pyrus_communis	pycom02g03540 pycom15g16350
rosa_chinensis	RchiOBHm_Chr2g0089861
rosa_laevigata	RLG00000016062
rosa_multiflora	Rmu_co8299847.1_g000001 Rmu_sc0002384.1_g000010
rosa_roxburghii	Rroxscaffold_2G00151530
rosa_rugosa	Rorug02G0003700
rosa_samantha	Rh2AG049000 Rh2BG047900 Rh2CG050000 Rh2DG049100
rosa_wichuraiana	Rw2G004120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	69, 5826, 7887
Acc16I	TGCGCA	2 cut(s)	2082, 5456
Acc36I	ACCTGC	1 cut(s)	5569
Acc65I	GGTACC	2 cut(s)	59, 5652
AccB1I	GGYRCC	4 cut(s)	59, 104, 3666, 5652
AccB7I	CCANNNNNTGG	7 cut(s)	20, 1199, 1700, 2777, 2983, 6821, 7097
AccBSI	CCGCTC	2 cut(s)	2489, 3847
AccI	GTMKAC	4 cut(s)	2449, 5609, 6720, 7230
AccIII	TCCGGA	1 cut(s)	3463
AcoI	YGGCCR	6 cut(s)	162, 436, 559, 1495, 2151, 4754
AcyI	GRCGYC	2 cut(s)	1270, 6434
AdeI	CACNNNGTG	2 cut(s)	2195, 5720
AflII	CTTAAG	3 cut(s)	5633, 6380, 7676
AflIII	ACRYGT	5 cut(s)	1888, 4768, 5306, 7459, 7511
AhdI	GACNNNNNGTC	2 cut(s)	5911, 6420
AhlI	ACTAGT	3 cut(s)	5518, 7333, 7535
AjiI	CACGTC	3 cut(s)	17, 280, 2438
AjuI	GAANNNNNNNTTGG	8 cut(s)	2814, 2846, 6069, 6101, 7686, 7718, 7764, 7796
AleI	CACNNNNGTG	1 cut(s)	3026
Alw21I	GWGCWC	7 cut(s)	89, 575, 3406, 3883, 5269, 5538, 7927
Alw44I	GTGCAC	1 cut(s)	5534
AlwNI	CAGNNNCTG	5 cut(s)	1604, 1622, 2309, 2954, 5225
Ama87I	CYCGRG	3 cut(s)	153, 463, 5967
Aor13HI	TCCGGA	1 cut(s)	3463
ApaI	GGGCCC	1 cut(s)	7834
ApaLI	GTGCAC	1 cut(s)	5534
ArsI	GACNNNNNNTTYG	4 cut(s)	1438, 1470, 6419, 6451
Asp700I	GAANNNNTTC	2 cut(s)	2292, 7698
Asp718I	GGTACC	2 cut(s)	59, 5652
AsuC2I	CCSGG	2 cut(s)	1641, 4326
AsuNHI	GCTAGC	4 cut(s)	4018, 5729, 7502, 7792
AvaI	CYCGRG	3 cut(s)	153, 463, 5967
AxyI	CCTNAGG	3 cut(s)	1728, 5109, 7551
BaeGI	GKGCMC	4 cut(s)	3364, 5538, 7329, 7834
BalI	TGGCCA	2 cut(s)	1497, 4756
BamHI	GGATCC	2 cut(s)	2767, 3032
BanI	GGYRCC	4 cut(s)	59, 104, 3666, 5652
BanII	GRGCYC	4 cut(s)	160, 575, 2857, 7834
BauI	CACGAG	3 cut(s)	427, 1149, 1407
BbsI	GAAGAC	5 cut(s)	2608, 3511, 4169, 4828, 7366
Bbv12I	GWGCWC	7 cut(s)	89, 575, 3406, 3883, 5269, 5538, 7927
BbvCI	CCTCAGC	1 cut(s)	3153
BcgI	CGANNNNNNTGC	4 cut(s)	3371, 3405, 4385, 4419
BciVI	GTATCC	5 cut(s)	2002, 2851, 4924, 6340, 7304
BclI	TGATCA	2 cut(s)	3085, 8065
BcnI	CCSGG	2 cut(s)	1641, 4326
BcuI	ACTAGT	3 cut(s)	5518, 7333, 7535
BfoI	RGCGCY	4 cut(s)	471, 4019, 5077, 5780
BfrI	CTTAAG	3 cut(s)	5633, 6380, 7676
BfuAI	ACCTGC	1 cut(s)	5569
BfuI	GTATCC	5 cut(s)	2002, 2851, 4924, 6340, 7304
BglI	GCCNNNNNGGC	1 cut(s)	3149
BglII	AGATCT	2 cut(s)	2756, 7003
BlpI	GCTNAGC	1 cut(s)	1653
BmcAI	AGTACT	1 cut(s)	7698
BmeRI	GACNNNNNGTC	2 cut(s)	5911, 6420
BmeT110I	CYCGRG	3 cut(s)	153, 463, 5967
BmgBI	CACGTC	3 cut(s)	17, 280, 2438
BmrI	ACTGGG	4 cut(s)	2207, 2859, 3237, 7051
BmtI	GCTAGC	4 cut(s)	4022, 5733, 7506, 7796
BmuI	ACTGGG	4 cut(s)	2207, 2859, 3237, 7051
BpiI	GAAGAC	5 cut(s)	2608, 3511, 4169, 4828, 7366
BplI	GAGNNNNNCTC	6 cut(s)	1641, 1673, 5254, 5286, 5256, 5288
Bpu10I	CCTNAGC	5 cut(s)	3003, 3153, 4050, 4191, 7053
Bpu1102I	GCTNAGC	1 cut(s)	1653
BpuEI	CTTGAG	8 cut(s)	2189, 3359, 3833, 4964, 6121, 6189, 6386, 6749
BpuMI	CCSGG	2 cut(s)	1641, 4326
BsaAI	YACGTR	5 cut(s)	256, 1856, 3992, 5307, 5765
BsaBI	GATNNNNATC	2 cut(s)	2478, 3090
BsaHI	GRCGYC	2 cut(s)	1270, 6434
BsaI	GGTCTC	4 cut(s)	804, 2993, 7607, 8017
BsaJI	CCNNGG	9 cut(s)	165, 191, 483, 2003, 2658, 4314, 4872, 5490, 7315
BsaWI	WCCGGW	4 cut(s)	77, 2639, 3035, 3463
Bse21I	CCTNAGG	3 cut(s)	1728, 5109, 7551
Bse3DI	GCAATG	9 cut(s)	978, 2181, 2372, 2769, 3570, 4521, 5150, 6097, 7197
Bse8I	GATNNNNATC	2 cut(s)	2478, 3090
BseAI	TCCGGA	1 cut(s)	3463
BseDI	CCNNGG	9 cut(s)	165, 191, 483, 2003, 2658, 4314, 4872, 5490, 7315
BseJI	GATNNNNATC	2 cut(s)	2478, 3090
BseMI	GCAATG	9 cut(s)	978, 2181, 2372, 2769, 3570, 4521, 5150, 6097, 7197
BsePI	GCGCGC	1 cut(s)	404
BseSI	GKGCMC	4 cut(s)	3364, 5538, 7329, 7834
BseX3I	CGGCCG	3 cut(s)	162, 436, 559
BseYI	CCCAGC	5 cut(s)	130, 694, 3957, 7724, 7949
BsgI	GTGCAG	5 cut(s)	3117, 4080, 5238, 5524, 6963
Bsh1285I	CGRYCG	3 cut(s)	165, 439, 562
BshNI	GGYRCC	4 cut(s)	59, 104, 3666, 5652
BsiEI	CGRYCG	3 cut(s)	165, 439, 562
BsiHKAI	GWGCWC	7 cut(s)	89, 575, 3406, 3883, 5269, 5538, 7927
BsiHKCI	CYCGRG	3 cut(s)	153, 463, 5967
BsiWI	CGTACG	1 cut(s)	516
BslFI	GGGAC	5 cut(s)	1691, 1774, 3588, 7058, 7865
BsmBI	CGTCTC	3 cut(s)	271, 286, 2602
BsmFI	GGGAC	5 cut(s)	1691, 1774, 3588, 7058, 7865
BsmI	GAATGC	3 cut(s)	2665, 6482, 7814
Bso31I	GGTCTC	4 cut(s)	804, 2993, 7607, 8017
BsoBI	CYCGRG	3 cut(s)	153, 463, 5967
Bsp120I	GGGCCC	1 cut(s)	7830
Bsp13I	TCCGGA	1 cut(s)	3463
Bsp1407I	TGTACA	3 cut(s)	5056, 5154, 6057
Bsp1720I	GCTNAGC	1 cut(s)	1653
Bsp68I	TCGCGA	2 cut(s)	6989, 7820
BspEI	TCCGGA	1 cut(s)	3463
BspHI	TCATGA	1 cut(s)	3775
BspMAI	CTGCAG	8 cut(s)	675, 1315, 1882, 4408, 5082, 5692, 5839, 6784
BspMI	ACCTGC	1 cut(s)	5569
BspOI	GCTAGC	4 cut(s)	4022, 5733, 7506, 7796
BspQI	GCTCTTC	4 cut(s)	203, 381, 1069, 5328
BspT107I	GGYRCC	4 cut(s)	59, 104, 3666, 5652
BspTI	CTTAAG	3 cut(s)	5633, 6380, 7676
BspTNI	GGTCTC	4 cut(s)	804, 2993, 7607, 8017
BsrBI	CCGCTC	2 cut(s)	2489, 3847
BsrDI	GCAATG	9 cut(s)	978, 2181, 2372, 2769, 3570, 4521, 5150, 6097, 7197
BsrGI	TGTACA	3 cut(s)	5056, 5154, 6057
BssECI	CCNNGG	9 cut(s)	165, 191, 483, 2003, 2658, 4314, 4872, 5490, 7315
BssHII	GCGCGC	1 cut(s)	404
BssNI	GRCGYC	2 cut(s)	1270, 6434
BssSI	CACGAG	3 cut(s)	427, 1149, 1407
BssT1I	CCWWGG	3 cut(s)	483, 4872, 7315
Bst2BI	CACGAG	3 cut(s)	427, 1149, 1407
BstACI	GRCGYC	2 cut(s)	1270, 6434
BstAFI	CTTAAG	3 cut(s)	5633, 6380, 7676
BstAPI	GCANNNNNTGC	9 cut(s)	1319, 1604, 2404, 2671, 2936, 5098, 5843, 6817, 7664
BstAUI	TGTACA	3 cut(s)	5056, 5154, 6057
BstBAI	YACGTR	5 cut(s)	256, 1856, 3992, 5307, 5765
BstDSI	CCRYGG	1 cut(s)	165
BstH2I	RGCGCY	4 cut(s)	471, 4019, 5077, 5780
BstMCI	CGRYCG	3 cut(s)	165, 439, 562
BstSLI	GKGCMC	4 cut(s)	3364, 5538, 7329, 7834
BstSNI	TACGTA	1 cut(s)	1856
BstV2I	GAAGAC	5 cut(s)	2608, 3511, 4169, 4828, 7366
BstX2I	RGATCY	7 cut(s)	2756, 2767, 3032, 4831, 6587, 7003, 7129
BstXI	CCANNNNNNTGG	2 cut(s)	2243, 7336
BstYI	RGATCY	7 cut(s)	2756, 2767, 3032, 4831, 6587, 7003, 7129
BstZI	CGGCCG	3 cut(s)	162, 436, 559
Bsu36I	CCTNAGG	3 cut(s)	1728, 5109, 7551
BsuI	GTATCC	5 cut(s)	2002, 2851, 4924, 6340, 7304
BtgI	CCRYGG	1 cut(s)	165
BtgZI	GCGATG	3 cut(s)	1484, 2379, 6974
BtrI	CACGTC	3 cut(s)	17, 280, 2438
BtsI	GCAGTG	5 cut(s)	3355, 3374, 4444, 4630, 5536
BtuMI	TCGCGA	2 cut(s)	6989, 7820
BveI	ACCTGC	1 cut(s)	5569
CaiI	CAGNNNCTG	5 cut(s)	1604, 1622, 2309, 2954, 5225
CciI	TCATGA	1 cut(s)	3775
CciNI	GCGGCCGC	1 cut(s)	436
Cfr42I	CCGCGG	1 cut(s)	168
CseI	GACGC	7 cut(s)	516, 1278, 2619, 3715, 5757, 6423, 8022
CsiI	ACCWGGT	1 cut(s)	6892
DraI	TTTAAA	3 cut(s)	760, 801, 5188
DraIII	CACNNNGTG	2 cut(s)	2195, 5720
DriI	GACNNNNNGTC	2 cut(s)	5911, 6420
EaeI	YGGCCR	6 cut(s)	162, 436, 559, 1495, 2151, 4754
EagI	CGGCCG	3 cut(s)	162, 436, 559
Eam1105I	GACNNNNNGTC	2 cut(s)	5911, 6420
EciI	GGCGGA	2 cut(s)	197, 294
Ecl136II	GAGCTC	1 cut(s)	573
EclXI	CGGCCG	3 cut(s)	162, 436, 559
Eco105I	TACGTA	1 cut(s)	1856
Eco130I	CCWWGG	3 cut(s)	483, 4872, 7315
Eco147I	AGGCCT	2 cut(s)	1974, 3143
Eco24I	GRGCYC	4 cut(s)	160, 575, 2857, 7834
Eco31I	GGTCTC	4 cut(s)	804, 2993, 7607, 8017
Eco32I	GATATC	2 cut(s)	3252, 3284
Eco52I	CGGCCG	3 cut(s)	162, 436, 559
Eco53kI	GAGCTC	1 cut(s)	573
Eco81I	CCTNAGG	3 cut(s)	1728, 5109, 7551
Eco88I	CYCGRG	3 cut(s)	153, 463, 5967
EcoICRI	GAGCTC	1 cut(s)	573
EcoO109I	RGGNCCY	4 cut(s)	3602, 5105, 7830, 7852
EcoRI	GAATTC	3 cut(s)	3062, 6394, 7861
EcoRV	GATATC	2 cut(s)	3252, 3284
EcoT14I	CCWWGG	3 cut(s)	483, 4872, 7315
EcoT22I	ATGCAT	7 cut(s)	1584, 1687, 1691, 5150, 5757, 6822, 7816
EcoT38I	GRGCYC	4 cut(s)	160, 575, 2857, 7834
ErhI	CCWWGG	3 cut(s)	483, 4872, 7315
Esp3I	CGTCTC	3 cut(s)	271, 286, 2602
FaqI	GGGAC	5 cut(s)	1691, 1774, 3588, 7058, 7865
FauI	CCCGC	4 cut(s)	258, 278, 2494, 7842
FauNDI	CATATG	4 cut(s)	922, 6028, 6048, 6553
FbaI	TGATCA	2 cut(s)	3085, 8065
FblI	GTMKAC	4 cut(s)	2449, 5609, 6720, 7230
FriOI	GRGCYC	4 cut(s)	160, 575, 2857, 7834
FspAI	RTGCGCAY	1 cut(s)	5456
FspI	TGCGCA	2 cut(s)	2082, 5456
GsaI	CCCAGC	5 cut(s)	134, 698, 3961, 7728, 7953
HaeII	RGCGCY	4 cut(s)	471, 4019, 5077, 5780
HgaI	GACGC	7 cut(s)	516, 1278, 2619, 3715, 5757, 6423, 8022
Hin1I	GRCGYC	2 cut(s)	1270, 6434
HincII	GTYRAC	6 cut(s)	2450, 3757, 5862, 7039, 7231, 8037
HindII	GTYRAC	6 cut(s)	2450, 3757, 5862, 7039, 7231, 8037
HindIII	AAGCTT	6 cut(s)	939, 3780, 4848, 5064, 6458, 6800
Hpy99I	CGWCG	9 cut(s)	524, 527, 532, 569, 738, 2259, 2439, 6994, 7825
Hsp92I	GRCGYC	2 cut(s)	1270, 6434
Kpn2I	TCCGGA	1 cut(s)	3463
KpnI	GGTACC	2 cut(s)	63, 5656
Ksp22I	TGATCA	2 cut(s)	3085, 8065
KspI	CCGCGG	1 cut(s)	168
LguI	GCTCTTC	4 cut(s)	203, 381, 1069, 5328
MabI	ACCWGGT	1 cut(s)	6892
MbiI	CCGCTC	2 cut(s)	2489, 3847
MflI	RGATCY	7 cut(s)	2756, 2767, 3032, 4831, 6587, 7003, 7129
MlsI	TGGCCA	2 cut(s)	1497, 4756
MluNI	TGGCCA	2 cut(s)	1497, 4756
Mox20I	TGGCCA	2 cut(s)	1497, 4756
Mph1103I	ATGCAT	7 cut(s)	1584, 1687, 1691, 5150, 5757, 6822, 7816
MroI	TCCGGA	1 cut(s)	3463
MroXI	GAANNNNTTC	2 cut(s)	2292, 7698
MscI	TGGCCA	2 cut(s)	1497, 4756
MslI	CAYNNNNRTG	7 cut(s)	1218, 1392, 2054, 3026, 3093, 4602, 6270
Msp20I	TGGCCA	2 cut(s)	1497, 4756
MspCI	CTTAAG	3 cut(s)	5633, 6380, 7676
Mva1269I	GAATGC	3 cut(s)	2665, 6482, 7814
NciI	CCSGG	2 cut(s)	1641, 4326
NdeI	CATATG	4 cut(s)	922, 6028, 6048, 6553
NheI	GCTAGC	4 cut(s)	4018, 5729, 7502, 7792
NotI	GCGGCCGC	1 cut(s)	436
NruI	TCGCGA	2 cut(s)	6989, 7820
NsbI	TGCGCA	2 cut(s)	2082, 5456
NsiI	ATGCAT	7 cut(s)	1584, 1687, 1691, 5150, 5757, 6822, 7816
OliI	CACNNNNGTG	1 cut(s)	3026
PaeI	GCATGC	4 cut(s)	1689, 2411, 5759, 7814
PaeR7I	CTCGAG	1 cut(s)	463
PagI	TCATGA	1 cut(s)	3775
PauI	GCGCGC	1 cut(s)	404
PceI	AGGCCT	2 cut(s)	1974, 3143
PciI	ACATGT	3 cut(s)	1888, 4768, 7511
PciSI	GCTCTTC	4 cut(s)	203, 381, 1069, 5328
PcsI	WCGNNNNNNNCGW	5 cut(s)	261, 564, 3997, 4006, 6120
PctI	GAATGC	3 cut(s)	2665, 6482, 7814
PdmI	GAANNNNTTC	2 cut(s)	2292, 7698
Pfl23II	CGTACG	1 cut(s)	516
PflMI	CCANNNNNTGG	7 cut(s)	20, 1199, 1700, 2777, 2983, 6821, 7097
PfoI	TCCNGGA	4 cut(s)	1168, 1639, 2518, 4324
Ppu21I	YACGTR	5 cut(s)	256, 1856, 3992, 5307, 5765
PpuMI	RGGWCCY	2 cut(s)	3602, 7852
PscI	ACATGT	3 cut(s)	1888, 4768, 7511
PsiI	TTATAA	3 cut(s)	69, 5826, 7887
Psp124BI	GAGCTC	1 cut(s)	575
Psp5II	RGGWCCY	2 cut(s)	3602, 7852
PspFI	CCCAGC	5 cut(s)	130, 694, 3957, 7724, 7949
PspLI	CGTACG	1 cut(s)	516
PspOMI	GGGCCC	1 cut(s)	7830
PspPPI	RGGWCCY	2 cut(s)	3602, 7852
PsrI	GAACNNNNNNTAC	2 cut(s)	5797, 5829
PstI	CTGCAG	8 cut(s)	675, 1315, 1882, 4408, 5082, 5692, 5839, 6784
PstNI	CAGNNNCTG	5 cut(s)	1604, 1622, 2309, 2954, 5225
PsuI	RGATCY	7 cut(s)	2756, 2767, 3032, 4831, 6587, 7003, 7129
PteI	GCGCGC	1 cut(s)	404
PvuII	CAGCTG	8 cut(s)	1604, 1823, 2891, 3770, 4511, 5840, 6698, 6779
RruI	TCGCGA	2 cut(s)	6989, 7820
RseI	CAYNNNNRTG	7 cut(s)	1218, 1392, 2054, 3026, 3093, 4602, 6270
SacI	GAGCTC	1 cut(s)	575
SacII	CCGCGG	1 cut(s)	168
SalI	GTCGAC	2 cut(s)	2448, 7229
SapI	GCTCTTC	4 cut(s)	203, 381, 1069, 5328
SbfI	CCTGCAGG	1 cut(s)	5082
ScaI	AGTACT	1 cut(s)	7698
SdaI	CCTGCAGG	1 cut(s)	5082
SexAI	ACCWGGT	1 cut(s)	6892
Sfr274I	CTCGAG	1 cut(s)	463
Sfr303I	CCGCGG	1 cut(s)	168
SgrBI	CCGCGG	1 cut(s)	168
SlaI	CTCGAG	1 cut(s)	463
SmiMI	CAYNNNNRTG	7 cut(s)	1218, 1392, 2054, 3026, 3093, 4602, 6270
SnaBI	TACGTA	1 cut(s)	1856
SpeI	ACTAGT	3 cut(s)	5518, 7333, 7535
SphI	GCATGC	4 cut(s)	1689, 2411, 5759, 7814
Sse8387I	CCTGCAGG	1 cut(s)	5082
SseBI	AGGCCT	2 cut(s)	1974, 3143
SspI	AATATT	4 cut(s)	4860, 4963, 5510, 7187
SstI	GAGCTC	1 cut(s)	575
StuI	AGGCCT	2 cut(s)	1974, 3143
StyI	CCWWGG	3 cut(s)	483, 4872, 7315
TaqII	GACCGA	2 cut(s)	680, 4840
TatI	WGTACW	7 cut(s)	5056, 5154, 6057, 6187, 7282, 7696, 7838
TspGWI	ACGGA	4 cut(s)	111, 253, 751, 7838
Van91I	CCANNNNNTGG	7 cut(s)	20, 1199, 1700, 2777, 2983, 6821, 7097
Vha464I	CTTAAG	3 cut(s)	5633, 6380, 7676
VneI	GTGCAC	1 cut(s)	5534
XbaI	TCTAGA	1 cut(s)	2504
XcmI	CCANNNNNNNNNTGG	9 cut(s)	17, 481, 1116, 4385, 4545, 5949, 6340, 6687, 6945
XhoI	CTCGAG	1 cut(s)	463
XmiI	GTMKAC	4 cut(s)	2449, 5609, 6720, 7230
XmnI	GAANNNNTTC	2 cut(s)	2292, 7698
ZrmI	AGTACT	1 cut(s)	7698
Zsp2I	ATGCAT	7 cut(s)	1584, 1687, 1691, 5150, 5757, 6822, 7816

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.