Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0010166

Physical Location & Seq

Genomic Coordinates Forward (+)

3878263 .. 3891996

Gene Structure 13734 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G004120.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 7764 bp

ATGGACCAACCTAACACCTCCACTGCTTCTTCCTCTTCCTCTTCCCCTTCTTCCCGTTCTGCCCCTGTCGAAGAGCCGGAGTACCTCGCGCGCTACTTAGTCGTCAAGCACTCGTGGCGCGGCCGCTACAAGCGGATTCTCTGCCTCTCGAGCGCCTCCATCGTCACCTTGGACCCGTCCACCCTCGCCGTCACGAACTCGTACGTCGTCGCGTCGGATTTCGACACCGCCGCCCCGATCATCGGCCGCGACGAGAGCTCCAGTGAGTTCAATTTGAGTGTTCGGACTGACGGCAGGGGGAAGTTCAAGTCGGTGAAGTTCTCGTCCAGGTATAGGGCGAGCATACTGACCGAGCTGCATAGGATTAGGGGCAATCGGCTTGGGGTAGTAGCGGAATTTCCAGTGCTTCATCTCCGGCGACGGACTGGCGAGTGGGTTCCTTTTAAACTAAAAGTTACTTATGTTGGGGTTGAACTTATTGATTTAAAATGCGGAGACCTCCGCTGGTGCTTGGATTTTAGAGATTTTCATTCCCCTGCCATTATTTCCCTTTCCGATGCTTACGGGAAGAAAGGCATTGAAGGAGGTTTTATTATTTGTCCTTCATATGGAAGAAAATCGAAAGCTTTTCAAGCTGCTTCTGGAACGACAAATTCTACTATCATTGCTAATTTGACTAAAACAGCAAAATCTATGGTCGGGGTGTCACTAAATGTGGAAACTTCTCAGTCTCTCACTATAGCAGAGTATATAAAGCGAAGAGCAAAAGAAGCAGTTGGAGCAGATGAAACTCCATGTGGAGGTTGGTCTGTGACAAGATTGCGCTCTGCTGCTCGTGGAACTCTAAATGTTCCAGGATTGAATTTGAGCGTTGGCCCAAAAGGTGGACTTGGGGAGCATGGTGATGCTGTATCTCGTCAATTGATTCTTACAAAAGTTTCACTTGTTGAAAGACGCCCAGAGAACTATGAAGCTGTCATCGTTCGACCTTTATCTGCAGTAAATGCCCTTGTTCGATTTACTGAGGAGCCTCAGATGTTTGCAATTGAGTTCAATGATGGATGCCCCATCCATGTATATGCAAGCACCTCTCGTGATAGCTTACTTGCAGCAGTTCGTGATCTGTTGCAAACAGAAGGTCAATGTGCTATGGCTGTGTTACCAAGACTGACGATGCCTGGCCATCGCATTGATCCACCTTGCGGTAGAGTTAATTTACAATTTGGACTACAACGGCCTATTGCAGATATGGAGAGTGCATCCATGCATTTGAAGCACTTAGCAGCAGCTGCCAAAGATGCAGTTTCTGAAGGTGGTTCCATTCCGGGATCAAGAGCTAAGCTCTGGCGCAGAATAAGAGAGTTCAATGCATGCATACCATATACTGGAGTCCCTCCTAACATAGAATTACCTGAGGTGACTTTGATGGCTTTAATAACAATGCTTCCAGCTACGCCAAATCTTCCTCCAGAGTCCCCTCCCTTGCCACCCCCTTCACCTAAAGCAGCTGCAACAGTCATGGGCTTTATTGCATGTTTACGTAGATTGTTGGCTTCAAGAACTGCAGCTTCACATGTCATGTCTTTTCCTGCTGCTGTTGGAAGAATAATGGGCTTACTCAGAAATGGTTCAGAGGGTGTAGCCGCTGAAGCTGCAGGCCTTATTGCAGTTCTTATTGGTGGTGGTCCTGGGGATACAAATATATTGACAGATTCTAAAGGTGAGCATTATGCCACAATCATGCACACAAAGTCAGTGTTGTTTGCGCAACAAGGTTATGTCATTATACTTGCCAATAGACTGAAGCCTATGTCTGTATCACCACTATTATCAATGGCCGTTGTTGAGGTTCTTGAGGCAATGATATGTGATCCACATGGTGAGACAACACAGTACCCAGTGTTTGTTGAACTATTACGCCAAGTCGCTGGTTTGAAACGTCGTTTGTTTGCATTGTTTGGACATCCTGCTGAAAGTGTTCGGGAAACAGTGGCTGTGATTATGCGTACAATTGCTGAAGAAGATGCAATTGCAGCAGAGTCCATGCGCGATGCTGCATTGCGTGATGGTGCTTTGTTGAGGCATTTATTGCACGCGTTCTTCCTTCCGGCTGGTGAGCGACGTGAGGTTAGTCGACAGCTTGTTGCTCTTTGGGCAGATTCGTATCAACCCGCTCTGGATTTATTGTCTAGAGTTCTGCCTCCTGGACTTGTTGCTTATTTGCACACAAAATCTGATGGAGTTCTATCAGAAGAGTCTAATCAAGAAGTTTCCTTGACCAGTAGAAGACAGAGACGCTTACTTCAACAGAGGAGAGGCCGTACCGGAAGAGGAGCAACATCCCAGGAGCATTCCTTGCCTTCTGTAAACAATTATGATGTGAATGACCCCATGACACAGACAAGTGGTGATGTCTCGAAAGTGTCAAATAACTATCAAAGATCTGCAATGGATCCAAATTCTGGACAGGCTCCGACCATTCAATCTTCTGGTGCTCAAACTGGTGAAAACTTGACTGGTGAAGTATCCTCTACTGGGGCTCCGCAAAGCAATAATACATCTATTGTGGCTTCAGCTGATGCTCCATCAACAGGTGTTCATGCTTCATTGGCAGCAAATACTGCAATCTCAACAGATTCTGACTCCAATGTTGCTGGTTTCCATAATACGGGTCTCCCTGCCCCTGCTCAGGTTGTTGTGGAAAACACTCCTGTGGGATCTGGTAGGCTTCTGTGTAATTGGCCTGAATTCTGGCGAGCATTTAGCCTTGATCATAATCGTGCAGATTTGATCTGGAATGAGCGTACCAGGCAAGAGTTAAGGGAGGCCTTGCAGGCTGAGGTTCATAACTTAGATGTTGAGAAGGAGCGTACAGAAGATATTGTTCCAAGAGGTTCTGCAGTAGAGATGGCTGGGCAAGATAGTGTGCCGCAGATATCTTGGAACTACTCTGAGTTCTCTGTCGGATATCCTAGTTTGTCTAAAGAAGTTTGTGTGGGTCAGTATTATCTCCGCTTGCTGCTTGAGAGTGGCAGTGGTGGCAGGGCACAAGAATTTCCACTGCGTGATCCTGTTGCCTTCTTTAGAGCACTCTATCATCGTTTCTTATGTGATGCAGACATAGGGCTTACAGTAGATGGTGCTGTTCCGGATGAAATGGGTGCATCTGATGATTGGTGCGACATGGGAAGACTAGATGGTTTTGGAGGTGGAGGGGGATTTTCAGTTAGGGAGCTTTGTGCAAGAGCAATGACAATTGTATATGAACAGCATTACAAAACAGTAGGTCCCTTTGAGGGTACTGCTCACATTACAGTTCTGTTGGACAGGACAGATGATAGGGCTTTGAGGCACCGCCTTCTGTTGCTTTTGAAGGCTTTAATGAAGGTGTTATCAAATGTGGAGGCTTGCGTCTTGGTTGGAGGATGTGTGCTAGGTGTTGACATGCTGACAGCTGTTCATGAAGCTTCTGAAAGGACGGCTATTCCTTTGCAATCCAACTTGATTGCTGCTACTGCCTTCATGGAACCGCTCAAGGAATGGATGTTTTTTGACAAGGAAGGAGCACAAGTTGGACCTGTTGAAAAGGATGCAATAAGAAGATTCTGGTCCAAGAAGGCAATTGATTGGACAACAAAGTGCTGGGCTTCTGGGATGCTAGACTGGAAGAGGTTACGTGATATTCGTGAACTTCGTTGGGCGCTAGCTGTTCGGGTTCCTGTTCTCACACCAGCTCAGGTTGGGGAGGCGGCATTGTCCATATTGCATAGCATGGTATCTGCACATTCTGATTTAGATGATGCTGGAGAAATAGTTACCCCAACTCCTAGAGTAAAAAGGATATTGTCAAGTCCGCGTTGTCTTCCGCATATTGCTCAGGCCATGCTTTCTGGAGAACCAAGTATTGTAGAGAGTGCTGCTGCATTACTGAAGGCTGTTGTTACCAGAAATCCGAAGGCCATGATTCGTCTATACAGCACAGGCGCATTTTATTTTTCCCTGGCATATCCTGGATCAAATCTCCTTTCAATTGCCCAACTATTTTCAGTGACTCATGTTCACCAAGCATTTCATGGTGGCGAAGATGCTGCAGTTTCCTCTTCCTTACCTCTGGCGAAACGCAGTGTATTGGGTGGGCTTCTTCCTGAATCTTTGCTGTATGTATTAGAGCGTAGTGGTCCAGGTGCATTTGCAGCTGCAATGGTATCTGATTCTGATACTCCAGAGATTATATGGACTCACAAGATGCGAGCAGATAATCTGATACGTCAGGTTTTGCAACATCTTGGTGACTTTCCCCAGAAATTGTCACAGCACTGCCATTGTCTATATGAGTATGCTCCTATGCCGCCAGTGACATACCCAGAGCTAAGAGATGAAATGTGGTGTCACCGTTATTACCTCCGCAACTTATGTGATGAGATTAGATTCCCAAATTGGCCAATTGTTGAACATGTTGAGTTTCTACAGTCATTACTGGTAATGTGGCGTGAGGAACTTACAAGAAGACCAATGGATCTTTCTGAGGAAGAAGCTTGCAAAATATTAGAGATTTCCTTGGAAGATGTGTCAAGTGATGATGCCAATATGAAACATTCTGTTGAGATGGTTGAGGATACGTCTAGCATATCCAAGCAGATTGAGAATATTGATGAAGAAAAGCTCAAGCGACAATATAGGAAACTTGCCATGAGATACCATCCAGACAAAAATCCTGAAGGAAGGGATAAGTTTCTTGCTGTACAGAAAGCTTATGAGCGCCTGCAGGCCACTATGCAAGGCTTGCAAGGCCCTCAGGCTTGGCGGTTGTTGCTTTTATTGAAAGGGCAATGCATCTTGTACAGAAGATATGGAGATATATTGGAACCGTTTAAATATGCTGGGTATCCGATGTTGCTCAGTGCAGTTACTGTGGACAAGGATGATAACAATTTTCTTTCCTTAGAGAGAGCACCTCTCCTTGTTGCAGCATCAGAGCTTATTTGGCTTACGTGTGCATCTTCTTCATTGAATGGTGAAGAGCTTGTGAGGGATGGTGGCATACAACTTCTTGGAAATCTTCTTTCCCGTTGCATGTGTGTAGTTCAGCCAACAACTCCTGCAAGTGAACCATCTGCCATAATTGTTACAAATGTTATGCACACCTTCTGTGTTTTGAGTCAATTTGAGAGTGCCTGGGCAGAGATACTCGAATATTCTGGACTAGTTGATGATATTGTGCACTGCACTGAGCTGGAGCTTGTACCTGCTGCTGTAGATGCTGCCCTCCAGACTATTGCCCATGTTTCTGTGTCTACTCAATTGCAAGATGCCTTGCTTAAGGCTGGTGTCTTATGGTACCTTTTACCCGTGCTGCTTCAGTATGACTCTACTGCAAAGGAATCTGATACAACAGAATCACATGGTGTTGGTGCTAGCGTACAGATTGCCAAAAATATGCATGCTATACGTGCATCACAGGCGCTATCAAGGCTTAGTGGTTTGTGTAGTAATGAGAGTTCAACACCTTATAATCAGACTGCAGCTGACGCCCTCAGAGCTTTGTTGACTCCAAAACTTGCCAGTATGTTAAAAGATCAAGCTCCTAAAGACCTACTGTCAAAATTAAACAATAACTTGGAGTCTCCAGAGATTATCTGGAACTCTTCCACCCGAGCAGAACTACTGAAGTTTGTGGATCAGCAACGTGCAAGTCAGGGTCCAGACGGCTCATATGACCTGAAAGATTCGCATTTGTTTGTGTACAAGGCACTGTCAAAAGAACTTTATGTTGGCAATGTTTACTTGAGGGTGTATAACAATCAGCCCGATTTTGATATTAGTGAACCAGAGGCTTTCTGTGTCGCTTTAATTGATTTTATATCATATCTAGTACACAATCAATGTTCTCAAGATTCAGAAGTTCACGATGAGCCAAAGCAGGATGGCTCATCCCTTGAAACATCTGAGCATCCTAATGATATGGCTATTAGATCAGTGGATGAACTTAGTGCTCCAGTTGAAGATTTGACATTATCCAACGGCAAAGTGGCAGACAAAGAAGAATCTAAAGTGGTCAAAAACCTTAAGTTTGCATTGAATTCACTCAAGAACATACTGACAAGCAGTCCAAATTTGGCGTCAATTTTTTCTACTAAAGACAAGCTTTTGCCTCTTTTTGAATGCTTCTCTGTGCCTGTTGCACCAGAAAGTAACATACCTCAACTTTGCCTGAGTGTGTTGTCACTCTTGACAACGTATGCTCCCTGCTTGGAGGCTATGGTTGCAGATGGATCTAGCCTTCTCCTTTTATTGCAAATGCTTCACTCAACCCCAAGTTGTCGTGAGGGGGTTCTTCATGTTCTATATGCTCTGGCAAGCACACCAGAGCTTGCTTGGGCAGCTGCCAAGCATGGAGGAGTGGTCTACATTCTTGAGCTTCTCTTGCCTTTGCAGGAAGAAATTTCCTTGCAGCAAAGAGCAGCTGCAGCCTCTTTGTTGGGGAAGCTTGTTGGGCAGCCAATGCATGGGCCAAGAGTTGCTATAACGCTAGCAAGGTTTCTTCCTGATGGCTTGGTATCAGTTATTAGGGATGGACCTGGTGAAGCTGTTGTAGTTGCCCTTGAACAGACTACTGAGACTCCAGAACTTGTATGGACACCAGCGATGGCTACTTCTCTGTCTGCACAAATCGCGACGATGGCAGCAGATCTATACCAAGAACAAATGAAAGGCCGTGTTGTTGACTGGGACGTACCTGAGCAGGCATCTGGGCAGCAAGAAATGAGAGATGAGCCGCAGGTTGGTGGAATATATATTAGGTTATTCTTAAAAGATCCTAAATTCCCTCTCAGAAATCCAAAGAGGTTCTTGGAAGGACTGCTAGATCAGTATTTGACATCCATTGCCGCCACACATTATGAAACACAAGCTGTCGACCCTGAACTTCCTTTGCTCCTGTCTGCTGCTTTGGTTTCATTATTGCGAGTACATCCTGCCCTTGCAGATCATGTTGGATACCTTGGATATGTGCCCAAACTAGTGGCTGCTGTGGCTTATGAAGGAAGACGAGAAACAATGGCATCGGGGGAGGTGAACAATGGAAACTATGTGGACAGAGCAGAGGAGTCTGATGATACACAGCCTGCCCAAACTCCCCAAGAACGTGTGCGCCTCAGTTGTCTGCGTGTTCTACATCAATTGGCAGCTAGCACCACATGTGCTGAAGCTATGGCAGCAACTAGTGTAGGAACACCTCAGGTTGTTCCTCTTCTAATGAAAGCTATAGGATGGCAAGGTGGAAGCATACTAGCTCTGGAGACCCTAAAGCGTGTTGTTGTTGCTGGAAACCGAGCTAGGGATGCACTCGTTGCACAAGGACTTAAGGTTGGTCTTGTTGAAGTACTTCTTGGCCTTCTTGATTGGAGAGCTGGGGGAAGGAATGGACTTTGCTCTCAGATGAAGTGGAATGAATCTGAAGCATCTATTGGCAGAGTGCTAGCAATTGAGGTTTTGCATGCATTCGCGACGGAGGGGGCCCACTGTACTAAAGTGCGGGACTTATTGAATTCCTCAGATGTTTGGAGTGCTTATAAAGATCAGAAACATGATCTTTTCCTTCCTTCAAGTGCTCAATCTGCTGCCGCTGGAATTGCTGGGCTAATAGAAAATTCATCATCAAGACTCACTTATGCCATTACAGCTCCTCCATCACAACCCTCTACATCGAGACCTCCAGCGTCAACTACATTTGAATCAAATGGAAGATATGATCAGCTATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005766 GO:0005768 GO:0005769 GO:0005773 GO:0005774 GO:0005829 GO:0005886 GO:0006810 GO:0006887 GO:0006897 GO:0006898 GO:0006955 GO:0006996 GO:0007032 GO:0008150 GO:0009987 GO:0010008 GO:0010256 GO:0012505 GO:0012506 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0030141 GO:0030659 GO:0030667 GO:0031090 GO:0031410 GO:0031901 GO:0031982 GO:0032386 GO:0032879 GO:0032940 GO:0035577 GO:0036230 GO:0042119 GO:0042582 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043299 GO:0043312 GO:0044422 GO:0044424 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0050789 GO:0050794 GO:0050896 GO:0051049 GO:0051179 GO:0051234 GO:0060341 GO:0060627 GO:0065007 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098657 GO:0098805 GO:0098852 GO:0099503 GO:1902954 GO:1903649 GO:2000641

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4339 DnaJ

Protein Analysis

2587

Amino Acids

282.61

Weight (kDa)

5.85

Isoelectric Point (pI)

46.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RME-8_N	PF19432	31 - 254	1.1e-22	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	300 - 754	7.2e-56	DNAJ protein RME-8 N-terminal
RME-8_N	PF19432	912 - 1010	1.6e-24	DNAJ protein RME-8 N-terminal
GYF_2	PF14237	1183 - 1231	1.8e-12	GYF domain 2
DnaJ	PF00226	1549 - 1589	4.1e-10	DnaJ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010166)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26890
fragaria_vesca	FvH4_1g04290 FvH4_1g04290 FvH4_1g04983
malus_domestica	MD02G1042600.v1.1 MD15G1181200.v1.1
prunus_persica	Prupe.7G234400_v2.0.a1 Prupe.7G234400_v2.0.a1
pyrus_communis	pycom02g03540 pycom15g16350
rosa_chinensis	RchiOBHm_Chr2g0089861
rosa_laevigata	RLG00000016062
rosa_multiflora	Rmu_co8299847.1_g000001 Rmu_sc0002384.1_g000010
rosa_roxburghii	Rroxscaffold_2G00151530
rosa_rugosa	Rorug02G0003700
rosa_samantha	Rh2AG049000 Rh2BG047900 Rh2CG050000 Rh2DG049100
rosa_wichuraiana	Rw2G004120

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	5511, 7572
Acc16I	TGCGCA	1 cut(s)	1767
Acc36I	ACCTGC	2 cut(s)	5254, 6769
Acc65I	GGTACC	1 cut(s)	5337
AccB1I	GGYRCC	2 cut(s)	3351, 5337
AccB7I	CCANNNNNTGG	4 cut(s)	884, 1385, 2462, 6506
AccBSI	CCGCTC	2 cut(s)	2174, 3532
AccI	GTMKAC	4 cut(s)	2134, 5294, 6405, 6915
AccIII	TCCGGA	1 cut(s)	3148
AcoI	YGGCCR	5 cut(s)	121, 244, 1180, 1836, 4439
AcyI	GRCGYC	3 cut(s)	955, 5529, 6119
AdeI	CACNNNGTG	3 cut(s)	1880, 3065, 5405
AflII	CTTAAG	3 cut(s)	5318, 6065, 7361
AflIII	ACRYGT	6 cut(s)	1573, 2094, 4453, 4991, 7144, 7196
AhdI	GACNNNNNGTC	2 cut(s)	5596, 6105
AhlI	ACTAGT	3 cut(s)	5203, 7018, 7220
AjiI	CACGTC	1 cut(s)	2123
AjuI	GAANNNNNNNTTGG	8 cut(s)	4504, 4536, 5754, 5786, 7371, 7403, 7449, 7481
AleI	CACNNNNGTG	1 cut(s)	2711
Alw21I	GWGCWC	8 cut(s)	260, 2497, 3091, 3568, 4954, 5223, 5995, 7612
Alw44I	GTGCAC	1 cut(s)	5219
AlwNI	CAGNNNCTG	5 cut(s)	1289, 1307, 1994, 2639, 4910
Ama87I	CYCGRG	2 cut(s)	148, 5652
Aor13HI	TCCGGA	1 cut(s)	3148
ApaI	GGGCCC	1 cut(s)	7519
ApaLI	GTGCAC	1 cut(s)	5219
ArsI	GACNNNNNNTTYG	4 cut(s)	1123, 1155, 6104, 6136
Asp700I	GAANNNNTTC	3 cut(s)	1627, 1977, 7383
Asp718I	GGTACC	1 cut(s)	5337
AsuC2I	CCSGG	1 cut(s)	1326
AsuNHI	GCTAGC	5 cut(s)	3703, 5414, 6529, 7187, 7477
AvaI	CYCGRG	2 cut(s)	148, 5652
AvaII	GGWCC	9 cut(s)	4, 172, 1685, 3287, 3575, 3609, 4178, 5699, 6575
AxyI	CCTNAGG	3 cut(s)	1413, 4794, 7236
BaeGI	GKGCMC	4 cut(s)	3049, 5223, 7014, 7519
BalI	TGGCCA	2 cut(s)	1182, 4441
BamHI	GGATCC	1 cut(s)	2452
BanI	GGYRCC	2 cut(s)	3351, 5337
BanII	GRGCYC	3 cut(s)	260, 2542, 7519
BauI	CACGAG	3 cut(s)	112, 834, 1092
BbsI	GAAGAC	5 cut(s)	2293, 3196, 3854, 4513, 7051
Bbv12I	GWGCWC	8 cut(s)	260, 2497, 3091, 3568, 4954, 5223, 5995, 7612
BbvCI	CCTCAGC	1 cut(s)	2838
BceAI	ACGGC	9 cut(s)	173, 307, 1250, 1823, 2304, 3495, 5722, 6037, 6699
BcgI	CGANNNNNNTGC	2 cut(s)	4070, 4104
BciVI	GTATCC	5 cut(s)	1687, 2536, 4609, 4896, 6989
BclI	TGATCA	2 cut(s)	2770, 7750
BcnI	CCSGG	1 cut(s)	1326
BcuI	ACTAGT	3 cut(s)	5203, 7018, 7220
BfoI	RGCGCY	4 cut(s)	156, 3704, 4762, 5465
BfrI	CTTAAG	3 cut(s)	5318, 6065, 7361
BfuAI	ACCTGC	2 cut(s)	5254, 6769
BfuI	GTATCC	5 cut(s)	1687, 2536, 4609, 4896, 6989
BglI	GCCNNNNNGGC	1 cut(s)	2834
BglII	AGATCT	2 cut(s)	2441, 6688
BlpI	GCTNAGC	1 cut(s)	1338
BmcAI	AGTACT	1 cut(s)	7383
Bme18I	GGWCC	9 cut(s)	4, 172, 1685, 3287, 3575, 3609, 4178, 5699, 6575
BmeRI	GACNNNNNGTC	2 cut(s)	5596, 6105
BmeT110I	CYCGRG	2 cut(s)	148, 5652
BmgBI	CACGTC	1 cut(s)	2123
BmrI	ACTGGG	3 cut(s)	1892, 2544, 6736
BmtI	GCTAGC	5 cut(s)	3707, 5418, 6533, 7191, 7481
BmuI	ACTGGG	3 cut(s)	1892, 2544, 6736
BpiI	GAAGAC	5 cut(s)	2293, 3196, 3854, 4513, 7051
BplI	GAGNNNNNCTC	6 cut(s)	1326, 1358, 4939, 4971, 4941, 4973
Bpu10I	CCTNAGC	5 cut(s)	2688, 2838, 3735, 3876, 6738
Bpu1102I	GCTNAGC	1 cut(s)	1338
BpuEI	CTTGAG	8 cut(s)	1874, 3044, 3518, 4649, 5806, 5874, 6071, 6434
BpuMI	CCSGG	1 cut(s)	1326
BsaAI	YACGTR	4 cut(s)	1541, 3677, 4992, 5450
BsaBI	GATNNNNATC	2 cut(s)	2163, 2775
BsaHI	GRCGYC	3 cut(s)	955, 5529, 6119
BsaI	GGTCTC	4 cut(s)	489, 2678, 7292, 7702
BsaJI	CCNNGG	7 cut(s)	168, 1688, 2343, 3999, 4557, 5175, 7000
BsaWI	WCCGGW	2 cut(s)	2324, 3148
Bse21I	CCTNAGG	3 cut(s)	1413, 4794, 7236
Bse3DI	GCAATG	9 cut(s)	663, 1866, 2057, 2454, 3255, 4206, 4835, 5782, 6882
Bse8I	GATNNNNATC	2 cut(s)	2163, 2775
BseAI	TCCGGA	1 cut(s)	3148
BseDI	CCNNGG	7 cut(s)	168, 1688, 2343, 3999, 4557, 5175, 7000
BseJI	GATNNNNATC	2 cut(s)	2163, 2775
BseMI	GCAATG	9 cut(s)	663, 1866, 2057, 2454, 3255, 4206, 4835, 5782, 6882
BsePI	GCGCGC	1 cut(s)	89
BseRI	GAGGAG	6 cut(s)	1040, 2326, 2346, 6411, 7118, 7674
BseSI	GKGCMC	4 cut(s)	3049, 5223, 7014, 7519
BseX3I	CGGCCG	2 cut(s)	121, 244
BseYI	CCCAGC	5 cut(s)	2912, 3642, 4880, 7409, 7634
BsgI	GTGCAG	5 cut(s)	2802, 3765, 4923, 5209, 6648
Bsh1285I	CGRYCG	2 cut(s)	124, 247
BshNI	GGYRCC	2 cut(s)	3351, 5337
BsiEI	CGRYCG	2 cut(s)	124, 247
BsiHKAI	GWGCWC	8 cut(s)	260, 2497, 3091, 3568, 4954, 5223, 5995, 7612
BsiHKCI	CYCGRG	2 cut(s)	148, 5652
BsiSI	CCGG	6 cut(s)	77, 415, 1325, 2108, 2325, 3149
BsiWI	CGTACG	1 cut(s)	201
BslFI	GGGAC	5 cut(s)	1376, 1459, 3273, 6743, 7550
BsmBI	CGTCTC	1 cut(s)	2287
BsmFI	GGGAC	5 cut(s)	1376, 1459, 3273, 6743, 7550
BsmI	GAATGC	3 cut(s)	2350, 6167, 7499
Bso31I	GGTCTC	4 cut(s)	489, 2678, 7292, 7702
BsoBI	CYCGRG	2 cut(s)	148, 5652
Bsp120I	GGGCCC	1 cut(s)	7515
Bsp13I	TCCGGA	1 cut(s)	3148
Bsp1407I	TGTACA	3 cut(s)	4741, 4839, 5742
Bsp1720I	GCTNAGC	1 cut(s)	1338
Bsp68I	TCGCGA	2 cut(s)	6674, 7505
BspEI	TCCGGA	1 cut(s)	3148
BspHI	TCATGA	1 cut(s)	3460
BspMAI	CTGCAG	8 cut(s)	1000, 1567, 1657, 2902, 4093, 4767, 5524, 6469
BspMI	ACCTGC	2 cut(s)	5254, 6769
BspOI	GCTAGC	5 cut(s)	3707, 5418, 6533, 7191, 7481
BspQI	GCTCTTC	3 cut(s)	66, 754, 5013
BspT107I	GGYRCC	2 cut(s)	3351, 5337
BspTI	CTTAAG	3 cut(s)	5318, 6065, 7361
BspTNI	GGTCTC	4 cut(s)	489, 2678, 7292, 7702
BsrBI	CCGCTC	2 cut(s)	2174, 3532
BsrDI	GCAATG	9 cut(s)	663, 1866, 2057, 2454, 3255, 4206, 4835, 5782, 6882
BsrGI	TGTACA	3 cut(s)	4741, 4839, 5742
BssECI	CCNNGG	7 cut(s)	168, 1688, 2343, 3999, 4557, 5175, 7000
BssHII	GCGCGC	1 cut(s)	89
BssNI	GRCGYC	3 cut(s)	955, 5529, 6119
BssSI	CACGAG	3 cut(s)	112, 834, 1092
BssT1I	CCWWGG	3 cut(s)	168, 4557, 7000
Bst2BI	CACGAG	3 cut(s)	112, 834, 1092
BstACI	GRCGYC	3 cut(s)	955, 5529, 6119
BstAFI	CTTAAG	3 cut(s)	5318, 6065, 7361
BstAPI	GCANNNNNTGC	8 cut(s)	1004, 1289, 2089, 2356, 2621, 4783, 6502, 7349
BstAUI	TGTACA	3 cut(s)	4741, 4839, 5742
BstBAI	YACGTR	4 cut(s)	1541, 3677, 4992, 5450
BstH2I	RGCGCY	4 cut(s)	156, 3704, 4762, 5465
BstMCI	CGRYCG	2 cut(s)	124, 247
BstNSI	RCATGY	9 cut(s)	1374, 1536, 1577, 3448, 4457, 5077, 5444, 7200, 7499
BstSLI	GKGCMC	4 cut(s)	3049, 5223, 7014, 7519
BstSNI	TACGTA	1 cut(s)	1541
BstV2I	GAAGAC	5 cut(s)	2293, 3196, 3854, 4513, 7051
BstX2I	RGATCY	7 cut(s)	2441, 2452, 2717, 4516, 6272, 6688, 6814
BstXI	CCANNNNNNTGG	2 cut(s)	1928, 7021
BstYI	RGATCY	7 cut(s)	2441, 2452, 2717, 4516, 6272, 6688, 6814
BstZI	CGGCCG	2 cut(s)	121, 244
Bsu36I	CCTNAGG	3 cut(s)	1413, 4794, 7236
BsuI	GTATCC	5 cut(s)	1687, 2536, 4609, 4896, 6989
BtgZI	GCGATG	3 cut(s)	1169, 2064, 6659
BtrI	CACGTC	1 cut(s)	2123
BtsI	GCAGTG	6 cut(s)	21, 3040, 3059, 4129, 4315, 5221
BtuMI	TCGCGA	2 cut(s)	6674, 7505
BveI	ACCTGC	2 cut(s)	5254, 6769
CaiI	CAGNNNCTG	5 cut(s)	1289, 1307, 1994, 2639, 4910
CciI	TCATGA	1 cut(s)	3460
CciNI	GCGGCCGC	1 cut(s)	121
CseI	GACGC	7 cut(s)	201, 963, 2304, 3400, 5537, 6108, 7707
CsiI	ACCWGGT	1 cut(s)	6577
DraI	TTTAAA	3 cut(s)	445, 486, 4873
DraIII	CACNNNGTG	3 cut(s)	1880, 3065, 5405
DriI	GACNNNNNGTC	2 cut(s)	5596, 6105
EaeI	YGGCCR	5 cut(s)	121, 244, 1180, 1836, 4439
EagI	CGGCCG	2 cut(s)	121, 244
Eam1105I	GACNNNNNGTC	2 cut(s)	5596, 6105
Ecl136II	GAGCTC	1 cut(s)	258
EclXI	CGGCCG	2 cut(s)	121, 244
Eco105I	TACGTA	1 cut(s)	1541
Eco130I	CCWWGG	3 cut(s)	168, 4557, 7000
Eco147I	AGGCCT	2 cut(s)	1659, 2828
Eco24I	GRGCYC	3 cut(s)	260, 2542, 7519
Eco31I	GGTCTC	4 cut(s)	489, 2678, 7292, 7702
Eco32I	GATATC	2 cut(s)	2937, 2969
Eco47I	GGWCC	9 cut(s)	4, 172, 1685, 3287, 3575, 3609, 4178, 5699, 6575
Eco52I	CGGCCG	2 cut(s)	121, 244
Eco53kI	GAGCTC	1 cut(s)	258
Eco81I	CCTNAGG	3 cut(s)	1413, 4794, 7236
Eco88I	CYCGRG	2 cut(s)	148, 5652
EcoICRI	GAGCTC	1 cut(s)	258
EcoO109I	RGGNCCY	3 cut(s)	3287, 4790, 7515
EcoRI	GAATTC	3 cut(s)	2747, 6079, 7546
EcoRV	GATATC	2 cut(s)	2937, 2969
EcoT14I	CCWWGG	3 cut(s)	168, 4557, 7000
EcoT22I	ATGCAT	7 cut(s)	1269, 1372, 1376, 4835, 5442, 6507, 7501
EcoT38I	GRGCYC	3 cut(s)	260, 2542, 7519
ErhI	CCWWGG	3 cut(s)	168, 4557, 7000
Esp3I	CGTCTC	1 cut(s)	2287
FaqI	GGGAC	5 cut(s)	1376, 1459, 3273, 6743, 7550
FauI	CCCGC	2 cut(s)	2179, 7527
FauNDI	CATATG	2 cut(s)	607, 5713
FbaI	TGATCA	2 cut(s)	2770, 7750
FblI	GTMKAC	4 cut(s)	2134, 5294, 6405, 6915
FriOI	GRGCYC	3 cut(s)	260, 2542, 7519
FspI	TGCGCA	1 cut(s)	1767
GsaI	CCCAGC	5 cut(s)	2916, 3646, 4884, 7413, 7638
HaeII	RGCGCY	4 cut(s)	156, 3704, 4762, 5465
HapII	CCGG	6 cut(s)	77, 415, 1325, 2108, 2325, 3149
HgaI	GACGC	7 cut(s)	201, 963, 2304, 3400, 5537, 6108, 7707
Hin1I	GRCGYC	3 cut(s)	955, 5529, 6119
HincII	GTYRAC	6 cut(s)	2135, 3442, 5547, 6724, 6916, 7722
HindII	GTYRAC	6 cut(s)	2135, 3442, 5547, 6724, 6916, 7722
HindIII	AAGCTT	6 cut(s)	624, 3465, 4533, 4749, 6143, 6485
HpaII	CCGG	6 cut(s)	77, 415, 1325, 2108, 2325, 3149
Hpy99I	CGWCG	9 cut(s)	209, 212, 217, 254, 423, 1944, 2124, 6679, 7510
Hsp92I	GRCGYC	3 cut(s)	955, 5529, 6119
Kpn2I	TCCGGA	1 cut(s)	3148
KpnI	GGTACC	1 cut(s)	5341
Ksp22I	TGATCA	2 cut(s)	2770, 7750
LguI	GCTCTTC	3 cut(s)	66, 754, 5013
MabI	ACCWGGT	1 cut(s)	6577
MbiI	CCGCTC	2 cut(s)	2174, 3532
MflI	RGATCY	7 cut(s)	2441, 2452, 2717, 4516, 6272, 6688, 6814
MlsI	TGGCCA	2 cut(s)	1182, 4441
MluI	ACGCGT	1 cut(s)	2094
MluNI	TGGCCA	2 cut(s)	1182, 4441
Mox20I	TGGCCA	2 cut(s)	1182, 4441
Mph1103I	ATGCAT	7 cut(s)	1269, 1372, 1376, 4835, 5442, 6507, 7501
MroI	TCCGGA	1 cut(s)	3148
MroXI	GAANNNNTTC	3 cut(s)	1627, 1977, 7383
MscI	TGGCCA	2 cut(s)	1182, 4441
MslI	CAYNNNNRTG	7 cut(s)	903, 1077, 1739, 2711, 2778, 4287, 5955
Msp20I	TGGCCA	2 cut(s)	1182, 4441
MspCI	CTTAAG	3 cut(s)	5318, 6065, 7361
MspI	CCGG	6 cut(s)	77, 415, 1325, 2108, 2325, 3149
Mva1269I	GAATGC	3 cut(s)	2350, 6167, 7499
NciI	CCSGG	1 cut(s)	1326
NdeI	CATATG	2 cut(s)	607, 5713
NheI	GCTAGC	5 cut(s)	3703, 5414, 6529, 7187, 7477
NotI	GCGGCCGC	1 cut(s)	121
NruI	TCGCGA	2 cut(s)	6674, 7505
NsbI	TGCGCA	1 cut(s)	1767
NsiI	ATGCAT	7 cut(s)	1269, 1372, 1376, 4835, 5442, 6507, 7501
NspI	RCATGY	9 cut(s)	1374, 1536, 1577, 3448, 4457, 5077, 5444, 7200, 7499
OliI	CACNNNNGTG	1 cut(s)	2711
PaeI	GCATGC	3 cut(s)	1374, 5444, 7499
PaeR7I	CTCGAG	1 cut(s)	148
PagI	TCATGA	1 cut(s)	3460
PauI	GCGCGC	1 cut(s)	89
PceI	AGGCCT	2 cut(s)	1659, 2828
PciI	ACATGT	3 cut(s)	1573, 4453, 7196
PciSI	GCTCTTC	3 cut(s)	66, 754, 5013
PcsI	WCGNNNNNNNCGW	3 cut(s)	249, 3682, 3691
PctI	GAATGC	3 cut(s)	2350, 6167, 7499
PdmI	GAANNNNTTC	3 cut(s)	1627, 1977, 7383
Pfl23II	CGTACG	1 cut(s)	201
PflMI	CCANNNNNTGG	4 cut(s)	884, 1385, 2462, 6506
PfoI	TCCNGGA	4 cut(s)	853, 1324, 2203, 4009
Ppu21I	YACGTR	4 cut(s)	1541, 3677, 4992, 5450
PpuMI	RGGWCCY	1 cut(s)	3287
PscI	ACATGT	3 cut(s)	1573, 4453, 7196
PsiI	TTATAA	2 cut(s)	5511, 7572
Psp124BI	GAGCTC	1 cut(s)	260
Psp5II	RGGWCCY	1 cut(s)	3287
PspFI	CCCAGC	5 cut(s)	2912, 3642, 4880, 7409, 7634
PspLI	CGTACG	1 cut(s)	201
PspOMI	GGGCCC	1 cut(s)	7515
PspPPI	RGGWCCY	1 cut(s)	3287
PsrI	GAACNNNNNNTAC	2 cut(s)	5482, 5514
PstI	CTGCAG	8 cut(s)	1000, 1567, 1657, 2902, 4093, 4767, 5524, 6469
PstNI	CAGNNNCTG	5 cut(s)	1289, 1307, 1994, 2639, 4910
PsuI	RGATCY	7 cut(s)	2441, 2452, 2717, 4516, 6272, 6688, 6814
PteI	GCGCGC	1 cut(s)	89
PvuII	CAGCTG	8 cut(s)	1289, 1508, 2576, 3455, 4196, 5525, 6383, 6464
RruI	TCGCGA	2 cut(s)	6674, 7505
RseI	CAYNNNNRTG	7 cut(s)	903, 1077, 1739, 2711, 2778, 4287, 5955
SacI	GAGCTC	1 cut(s)	260
SalI	GTCGAC	2 cut(s)	2133, 6914
SapI	GCTCTTC	3 cut(s)	66, 754, 5013
SbfI	CCTGCAGG	1 cut(s)	4767
ScaI	AGTACT	1 cut(s)	7383
SdaI	CCTGCAGG	1 cut(s)	4767
SexAI	ACCWGGT	1 cut(s)	6577
Sfr274I	CTCGAG	1 cut(s)	148
SinI	GGWCC	9 cut(s)	4, 172, 1685, 3287, 3575, 3609, 4178, 5699, 6575
SlaI	CTCGAG	1 cut(s)	148
SmiMI	CAYNNNNRTG	7 cut(s)	903, 1077, 1739, 2711, 2778, 4287, 5955
SnaBI	TACGTA	1 cut(s)	1541
SpeI	ACTAGT	3 cut(s)	5203, 7018, 7220
SphI	GCATGC	3 cut(s)	1374, 5444, 7499
Sse8387I	CCTGCAGG	1 cut(s)	4767
SseBI	AGGCCT	2 cut(s)	1659, 2828
SspI	AATATT	3 cut(s)	4545, 4648, 5195
SstI	GAGCTC	1 cut(s)	260
StuI	AGGCCT	2 cut(s)	1659, 2828
StyI	CCWWGG	3 cut(s)	168, 4557, 7000
TaqII	GACCGA	1 cut(s)	365
TatI	WGTACW	7 cut(s)	4741, 4839, 5742, 5872, 6967, 7381, 7523
TspGWI	ACGGA	2 cut(s)	436, 7523
Van91I	CCANNNNNTGG	4 cut(s)	884, 1385, 2462, 6506
Vha464I	CTTAAG	3 cut(s)	5318, 6065, 7361
VneI	GTGCAC	1 cut(s)	5219
VpaK11BI	GGWCC	9 cut(s)	4, 172, 1685, 3287, 3575, 3609, 4178, 5699, 6575
XbaI	TCTAGA	1 cut(s)	2189
XceI	RCATGY	9 cut(s)	1374, 1536, 1577, 3448, 4457, 5077, 5444, 7200, 7499
XcmI	CCANNNNNNNNNTGG	8 cut(s)	166, 801, 4070, 4230, 5634, 6025, 6372, 6630
XhoI	CTCGAG	1 cut(s)	148
XmiI	GTMKAC	4 cut(s)	2134, 5294, 6405, 6915
XmnI	GAANNNNTTC	3 cut(s)	1627, 1977, 7383
ZrmI	AGTACT	1 cut(s)	7383
Zsp2I	ATGCAT	7 cut(s)	1269, 1372, 1376, 4835, 5442, 6507, 7501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.