MD15G1207000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013232
Physical Location & Seq
Reverse (-)
16473249 .. 16474069
821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1207000.v1.1.491

Sequence Viewer

Length: 756 bp
ATGGGGACCCACAAATATGAGTCCATAAATCCTTTTTCCTTTTTTTCCTCCGCTTGCGGTGTGGGCCACCCGATAAATACGACAGTACACACCACACCGAGCGCGCGCGCACACTCCACAGACACTCGCAAGAGACCCGGAAGCGAAGAGGCGGAAGACGAGGGGAGCGCCGCGAAGAAGCAGCGAGATCTGGCGGCTGAGAAAGTGGAAAACGACGACGTTAAGACTATGCTAACCAAGGCAAACGGCGGGGCGCTCGAGGAGCTCGCGAAGCTCGTGAACAAAGACTGGCCGATCGTCCCCGCTGCAGGTGCCACGGCGGTGACTTTCGTCGAGAACCCGTACTCCTCGTCGTGGGTGTTCCAAGCGCTGCCCTCGTCGTACGTCACGATCAACCTCAACGAGGAGAGCTGTGGGCCGGCGTTCTCGGACTCGGTGTCGACTGTCATGGCGAGCGTCGACCTGTGCGGCGGGCTGTACGGCATTGCGAAGGAGGTGCTGGGGTTGGAGTCGGAGAAGTGGTTGATGGAGGAGGTGGAGGAGGTCAGCGGTTTGTACGGGCTTGCAAAGGAGGTTATCGGCTTGGAATCTTTGAAGTTGGTAATGGCGGAGGAGGAAGAGGCGAGTGGGTTCGGCGGCGCGTGTAGTGAAACAAAAGAGTTGGCGCGTAGTGGTTGGGCGGGGGTGGGGGTAGAGATGGACTGGGACGATGCTGCGCTGGCGAGGTTTATCGGGGAAGACGGAGACCTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

26.72

Weight (kDa)

4.46

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013232)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56880
fragaria_vesca FvH4_1g07430 FvH4_1g21692
malus_domestica MD02G1079500.v1.1 MD15G1207000.v1.1
prunus_persica Prupe.7G209700_v2.0.a1
pyrus_communis pycom02g06330
rosa_laevigata RLG00000016392
rosa_multiflora Rmu_sc0005227.1_g000006 Rmu_sc0005227.1_g000007 Rmu_sc0005227.1_g000008
rosa_roxburghii Rroxscaffold_2G00148170
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081100 Rh2BG082200 Rh2CG084400 Rh2DG080100
rosa_wichuraiana Rw2G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 299
Acc36I ACCTGC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 311
AccI GTMKAC 2 cut(s) 440, 459
AccII CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
AcoI YGGCCR 1 cut(s) 290
AfaI GTAC 5 cut(s) 87, 344, 383, 479, 557
AfeI AGCGCT 1 cut(s) 369
AfiI CCNNNNNNNGG 3 cut(s) 308, 354, 403
AgsI TTSAA 1 cut(s) 595
AhdI GACNNNNNGTC 1 cut(s) 436
AleI CACNNNNGTG 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 329, 361
AluBI AGCT 3 cut(s) 265, 274, 411
AluI AGCT 3 cut(s) 265, 274, 411
Alw21I GWGCWC 1 cut(s) 267
Alw26I GTCTC 2 cut(s) 127, 738
Ama87I CYCGRG 1 cut(s) 257
Aor51HI AGCGCT 1 cut(s) 369
AoxI GGCC 3 cut(s) 64, 290, 416
ApeKI GCWGC 4 cut(s) 181, 305, 370, 713
AspS9I GGNCC 3 cut(s) 6, 64, 416
AsuC2I CCSGG 1 cut(s) 138
AsuHPI GGTGA 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 257
AvaII GGWCC 1 cut(s) 6
BanI GGYRCC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 267
BauI CACGAG 1 cut(s) 275
BbsI GAAGAC 2 cut(s) 162, 744
Bbv12I GWGCWC 1 cut(s) 267
BbvI GCAGC 4 cut(s) 193, 292, 357, 700
BccI CCATC 2 cut(s) 520, 691
BceAI ACGGC 3 cut(s) 262, 333, 496
BcgI CGANNNNNNTGC 2 cut(s) 478, 512
BcnI CCSGG 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 127, 738
BfmI CTRYAG 1 cut(s) 306
BfoI RGCGCY 3 cut(s) 171, 257, 371
BfuAI ACCTGC 1 cut(s) 299
BglII AGATCT 1 cut(s) 187
BisI GCNGC 8 cut(s) 171, 182, 195, 306, 371, 469, 637, 714
BlsI GCNGC 8 cut(s) 172, 183, 196, 307, 372, 470, 638, 715
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 6
BmeRI GACNNNNNGTC 1 cut(s) 436
BmeT110I CYCGRG 1 cut(s) 257
BmgT120I GGNCC 3 cut(s) 6, 64, 416
BmiI GGNNCC 3 cut(s) 7, 8, 313
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 712
BmsI GCATC 1 cut(s) 700
BmuI ACTGGG 1 cut(s) 712
BoxI GACNNNNGTC 1 cut(s) 329
BpiI GAAGAC 2 cut(s) 162, 744
BpuMI CCSGG 1 cut(s) 138
BsaI GGTCTC 2 cut(s) 127, 738
BsaJI CCNNGG 2 cut(s) 237, 315
BsaXI ACNNNNNCTCC 4 cut(s) 329, 359, 506, 536
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 354, 403
Bse118I RCCGGY 1 cut(s) 418
Bse1I ACTGG 2 cut(s) 293, 707
Bse3DI GCAATG 1 cut(s) 483
BseDI CCNNGG 2 cut(s) 237, 315
BseLI CCNNNNNNNGG 3 cut(s) 308, 354, 403
BseMI GCAATG 1 cut(s) 483
BseMII CTCAG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 293, 707
BsePI GCGCGC 3 cut(s) 102, 104, 106
BseRI GAGGAG 6 cut(s) 275, 337, 419, 545, 554, 626
BseXI GCAGC 4 cut(s) 193, 292, 357, 700
BseYI CCCAGC 1 cut(s) 499
Bsh1236I CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 66, 292, 418
BshNI GGYRCC 1 cut(s) 311
BsiEI CGRYCG 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 267
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 2 cut(s) 138, 419
BsiWI CGTACG 1 cut(s) 381
BslFI GGGAC 3 cut(s) 19, 284, 719
BslI CCNNNNNNNGG 3 cut(s) 308, 354, 403
BsmAI GTCTC 2 cut(s) 127, 738
BsmFI GGGAC 3 cut(s) 19, 284, 719
BsnI GGCC 3 cut(s) 66, 292, 418
Bso31I GGTCTC 2 cut(s) 127, 738
BsoBI CYCGRG 1 cut(s) 257
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 3 cut(s) 187, 294, 390
Bsp68I TCGCGA 1 cut(s) 269
BspANI GGCC 3 cut(s) 66, 292, 418
BspCNI CTCAG 1 cut(s) 190
BspFNI CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
BspLI GGNNCC 3 cut(s) 7, 8, 313
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 1 cut(s) 299
BspT107I GGYRCC 1 cut(s) 311
BspTNI GGTCTC 2 cut(s) 127, 738
BsrDI GCAATG 1 cut(s) 483
BsrFI RCCGGY 1 cut(s) 418
BsrI ACTGG 2 cut(s) 293, 707
BssAI RCCGGY 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 237, 315
BssHII GCGCGC 3 cut(s) 102, 104, 106
BssMI GATC 3 cut(s) 187, 294, 390
BssSI CACGAG 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 237
Bst2BI CACGAG 1 cut(s) 275
Bst4CI ACNGT 2 cut(s) 85, 445
Bst6I CTCTTC 2 cut(s) 141, 612
BstDEI CTNAG 1 cut(s) 198
BstDSI CCRYGG 1 cut(s) 315
BstENI CCTNNNNNAGG 1 cut(s) 401
BstFNI CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
BstH2I RGCGCY 3 cut(s) 171, 257, 371
BstKTI GATC 3 cut(s) 190, 297, 393
BstMAI GTCTC 2 cut(s) 127, 738
BstMBI GATC 3 cut(s) 187, 294, 390
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 5 cut(s) 63, 262, 271, 311, 719
BstPAI GACNNNNGTC 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
BstV1I GCAGC 4 cut(s) 193, 292, 357, 700
BstV2I GAAGAC 2 cut(s) 162, 744
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 3 cut(s) 66, 292, 418
BtgI CCRYGG 1 cut(s) 315
BtuMI TCGCGA 1 cut(s) 269
BveI ACCTGC 1 cut(s) 299
Cfr10I RCCGGY 1 cut(s) 418
Cfr13I GGNCC 3 cut(s) 6, 64, 416
CseI GACGC 1 cut(s) 445
Csp6I GTAC 5 cut(s) 86, 343, 382, 478, 556
CviAII CATG 1 cut(s) 448
CviQI GTAC 5 cut(s) 86, 343, 382, 478, 556
DdeI CTNAG 1 cut(s) 198
DpnI GATC 3 cut(s) 189, 296, 392
DpnII GATC 3 cut(s) 187, 294, 390
DriI GACNNNNNGTC 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 290
Eam1104I CTCTTC 2 cut(s) 141, 612
Eam1105I GACNNNNNGTC 1 cut(s) 436
EarI CTCTTC 2 cut(s) 141, 612
EciI GGCGGA 2 cut(s) 167, 623
Ecl136II GAGCTC 1 cut(s) 265
Eco130I CCWWGG 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 267
Eco31I GGTCTC 2 cut(s) 127, 738
Eco47I GGWCC 1 cut(s) 6
Eco47III AGCGCT 1 cut(s) 369
Eco53kI GAGCTC 1 cut(s) 265
Eco88I CYCGRG 1 cut(s) 257
EcoICRI GAGCTC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 237
EcoT38I GRGCYC 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 237
FaeI CATG 1 cut(s) 451
FaiI YATR 4 cut(s) 18, 26, 230, 449
FaqI GGGAC 3 cut(s) 19, 284, 719
FatI CATG 1 cut(s) 447
FauI CCCGC 4 cut(s) 242, 310, 464, 673
FblI GTMKAC 2 cut(s) 440, 459
Fnu4HI GCNGC 8 cut(s) 171, 182, 195, 306, 371, 469, 637, 714
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 8 cut(s) 171, 182, 195, 306, 371, 469, 637, 714
GluI GCNGC 8 cut(s) 171, 182, 195, 306, 371, 469, 637, 714
GsaI CCCAGC 1 cut(s) 503
HaeII RGCGCY 3 cut(s) 171, 257, 371
HaeIII GGCC 3 cut(s) 66, 292, 418
HapII CCGG 2 cut(s) 138, 419
HgaI GACGC 1 cut(s) 445
Hin1II CATG 1 cut(s) 451
HincII GTYRAC 2 cut(s) 441, 460
HindII GTYRAC 2 cut(s) 441, 460
HinfI GANTC 4 cut(s) 20, 431, 509, 587
HpaII CCGG 2 cut(s) 138, 419
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 4 cut(s) 88, 280, 441, 460
Hpy188I TCNGA 2 cut(s) 430, 514
Hpy188III TCNNGA 4 cut(s) 268, 277, 334, 388
Hpy8I GTNNAC 4 cut(s) 88, 280, 441, 460
Hpy99I CGWCG 6 cut(s) 218, 221, 335, 355, 382, 461
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 85, 445
HpyCH4IV ACGT 2 cut(s) 219, 384
HpyCH4V TGCA 2 cut(s) 308, 566
HpyF10VI GCNNNNNNNGC 5 cut(s) 63, 262, 271, 311, 719
HpyF3I CTNAG 1 cut(s) 198
HpySE526I ACGT 2 cut(s) 219, 384
Hsp92II CATG 1 cut(s) 451
KflI GGGWCCC 1 cut(s) 6
KroI GCCGGC 1 cut(s) 418
KroNI GCCGGC 1 cut(s) 420
Kzo9I GATC 3 cut(s) 187, 294, 390
LmnI GCTCC 2 cut(s) 165, 262
LpnPI CCDG 9 cut(s) 151, 176, 274, 294, 432, 476, 485, 688, 704
Lsp1109I GCAGC 4 cut(s) 193, 292, 357, 700
LweI GCATC 1 cut(s) 700
MaeII ACGT 2 cut(s) 219, 384
MaeIII GTNAC 2 cut(s) 322, 385
MalI GATC 3 cut(s) 189, 296, 392
MauBI CGCGCGCG 1 cut(s) 104
MboI GATC 3 cut(s) 187, 294, 390
MboII GAAGA 5 cut(s) 158, 167, 187, 629, 749
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 267
MluCI AATT 1 cut(s) 750
MlyI GAGTC 3 cut(s) 29, 425, 518
MmeI TCCRAC 2 cut(s) 486, 492
MroNI GCCGGC 1 cut(s) 418
MseI TTAA 1 cut(s) 222
MslI CAYNNNNRTG 2 cut(s) 15, 320
MspA1I CMGCKG 2 cut(s) 305, 549
MspI CCGG 2 cut(s) 138, 419
MspR9I CCNGG 1 cut(s) 138
MvnI CGCG 7 cut(s) 104, 106, 108, 173, 269, 641, 667
MwoI GCNNNNNNNGC 5 cut(s) 63, 262, 271, 311, 719
NaeI GCCGGC 1 cut(s) 420
NciI CCSGG 1 cut(s) 138
NdeII GATC 3 cut(s) 187, 294, 390
NgoMIV GCCGGC 1 cut(s) 418
NlaIII CATG 1 cut(s) 451
NlaIV GGNNCC 3 cut(s) 7, 8, 313
NmuCI GTSAC 2 cut(s) 322, 385
NruI TCGCGA 1 cut(s) 269
OliI CACNNNNGTG 1 cut(s) 320
PaeR7I CTCGAG 1 cut(s) 257
PaqCI CACCTGC 1 cut(s) 299
PauI GCGCGC 3 cut(s) 102, 104, 106
PcsI WCGNNNNNNNCGW 2 cut(s) 273, 386
PdiI GCCGGC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 587
Pfl23II CGTACG 1 cut(s) 381
PkrI GCNGC 8 cut(s) 172, 183, 196, 307, 372, 470, 638, 715
Ple19I CGATCG 1 cut(s) 297
PleI GAGTC 3 cut(s) 28, 425, 517
PpsI GAGTC 3 cut(s) 28, 425, 517
PpuMI RGGWCCY 1 cut(s) 6
PshAI GACNNNNGTC 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 267
Psp5II RGGWCCY 1 cut(s) 6
PspFI CCCAGC 1 cut(s) 499
PspLI CGTACG 1 cut(s) 381
PspN4I GGNNCC 3 cut(s) 7, 8, 313
PspPI GGNCC 3 cut(s) 6, 64, 416
PspPPI RGGWCCY 1 cut(s) 6
PspXI VCTCGAGB 1 cut(s) 257
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 187
PteI GCGCGC 3 cut(s) 102, 104, 106
PvuI CGATCG 1 cut(s) 297
RruI TCGCGA 1 cut(s) 269
RsaI GTAC 5 cut(s) 87, 344, 383, 479, 557
RsaNI GTAC 5 cut(s) 86, 343, 382, 478, 556
RseI CAYNNNNRTG 2 cut(s) 15, 320
SacI GAGCTC 1 cut(s) 267
SalI GTCGAC 2 cut(s) 439, 458
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 8 cut(s) 171, 182, 195, 306, 371, 469, 637, 714
Sau3AI GATC 3 cut(s) 187, 294, 390
Sau96I GGNCC 3 cut(s) 6, 64, 416
SchI GAGTC 3 cut(s) 29, 425, 518
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 267
SfaNI GCATC 1 cut(s) 700
SfcI CTRYAG 1 cut(s) 306
Sfr274I CTCGAG 1 cut(s) 257
SinI GGWCC 1 cut(s) 6
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 2 cut(s) 15, 320
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 1 cut(s) 750
SstI GAGCTC 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 237
TaaI ACNGT 2 cut(s) 85, 445
TaiI ACGT 2 cut(s) 222, 387
TaqI TCGA 4 cut(s) 258, 333, 440, 459
TasI AATT 1 cut(s) 750
TatI WGTACW 1 cut(s) 85
TauI GCSGC 4 cut(s) 173, 197, 471, 639
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TseFI GTSAC 2 cut(s) 322, 385
TseI GCWGC 4 cut(s) 181, 305, 370, 713
Tsp45I GTSAC 2 cut(s) 322, 385
TspGWI ACGGA 1 cut(s) 756
VpaK11BI GGWCC 1 cut(s) 6
XagI CCTNNNNNAGG 1 cut(s) 401
XhoI CTCGAG 1 cut(s) 257
XmiI GTMKAC 2 cut(s) 440, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.