Rmu_sc0005227.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005227.1
OG0013232
Physical Location & Seq
Reverse (-)
34381 .. 35710
1330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005227.1_g000008.1.cds

Sequence Viewer

Length: 597 bp
atgaatggtgaattgggtttcttgtatggaaactctgagccagaagctagcgaaggccacctcaacattcccttcatcttcatttggatttgctgggcgccggagacggggagaggaggatcagaggaagagaagaaaaagagtgacgtcgtcgttgcggacagaggcgacgtttcggttactccggcggtgaacgacgtcgttgcggacaacggcgacattttggctcctccggcagtgaacggcgtcgttgcggacagtggagacgcttcggttcctccggccgcgaacgacgtctcattctatataaattgcttgtcaaatgattttctctttacaattgcggagagcggcgacgagtcggcgagcgagctctcgaggcttctggagacggactacctgtcgcggaactcggtcgctgctccggcgagattgacgttctcggagcacccgtccaggtcgccgaagctgttccagacgatgtcgacttcgtacatcacgataaacgggaacgaggagagttgcggctcgtcgttctcggacgccgagtcgtcggtgatggcgagcgtggacaggacagggttaatagcaaaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

20.98

Weight (kDa)

4.21

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013232)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56880
fragaria_vesca FvH4_1g07430 FvH4_1g21692
malus_domestica MD02G1079500.v1.1 MD15G1207000.v1.1
prunus_persica Prupe.7G209700_v2.0.a1
pyrus_communis pycom02g06330
rosa_laevigata RLG00000016392
rosa_multiflora Rmu_sc0005227.1_g000006 Rmu_sc0005227.1_g000007 Rmu_sc0005227.1_g000008
rosa_roxburghii Rroxscaffold_2G00148170
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081100 Rh2BG082200 Rh2CG084400 Rh2DG080100
rosa_wichuraiana Rw2G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 150, 201, 297
AccB1I GGYRCC 1 cut(s) 97
AccBSI CCGCTC 1 cut(s) 351
AccI GTMKAC 1 cut(s) 485
AccII CGCG 2 cut(s) 287, 406
AciI CCGC 9 cut(s) 158, 188, 206, 254, 285, 344, 351, 406, 525
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 1 cut(s) 282
AcyI GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
AfaI GTAC 1 cut(s) 494
AfiI CCNNNNNNNGG 1 cut(s) 107
AhdI GACNNNNNGTC 2 cut(s) 400, 547
AjnI CCWGG 1 cut(s) 455
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 3 cut(s) 47, 373, 469
AluI AGCT 3 cut(s) 47, 373, 469
Alw21I GWGCWC 2 cut(s) 375, 450
Alw26I GTCTC 4 cut(s) 98, 258, 301, 383
AlwI GGATC 1 cut(s) 127
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 2 cut(s) 55, 282
ApeKI GCWGC 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 470
AspLEI GCGC 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 20, 202, 568
AsuNHI GCTAGC 1 cut(s) 47
AvaI CYCGRG 1 cut(s) 376
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 375
Bbv12I GWGCWC 2 cut(s) 375, 450
BbvI GCAGC 1 cut(s) 406
BccI CCATC 1 cut(s) 553
BceAI ACGGC 2 cut(s) 229, 259
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 4 cut(s) 98, 258, 301, 383
BfaI CTAG 1 cut(s) 48
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 4 cut(s) 285, 352, 420, 526
BlsI GCNGC 4 cut(s) 286, 353, 421, 527
Bme1390I CCNGG 1 cut(s) 457
BmeRI GACNNNNNGTC 2 cut(s) 400, 547
BmeT110I CYCGRG 1 cut(s) 376
BmiI GGNNCC 3 cut(s) 99, 228, 276
BmrFI CCNGG 1 cut(s) 457
BmtI GCTAGC 1 cut(s) 51
BpmI CTGGAG 1 cut(s) 407
BsaHI GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 457
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 27
BseRI GAGGAG 3 cut(s) 129, 219, 530
BseX3I CGGCCG 1 cut(s) 282
BseXI GCAGC 1 cut(s) 406
BseYI CCCAGC 1 cut(s) 93
Bsh1236I CGCG 2 cut(s) 287, 406
Bsh1285I CGRYCG 2 cut(s) 285, 417
BshFI GGCC 2 cut(s) 57, 284
BshNI GGYRCC 1 cut(s) 97
BsiEI CGRYCG 2 cut(s) 285, 417
BsiHKAI GWGCWC 2 cut(s) 375, 450
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 5 cut(s) 101, 185, 233, 281, 425
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 4 cut(s) 98, 258, 301, 383
BsmBI CGTCTC 4 cut(s) 98, 258, 301, 383
BsnI GGCC 2 cut(s) 57, 284
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 2 cut(s) 375, 450
Bsp143I GATC 1 cut(s) 119
BspACI CCGC 9 cut(s) 158, 188, 206, 254, 285, 344, 351, 406, 525
BspANI GGCC 2 cut(s) 57, 284
BspCNI CTCAG 1 cut(s) 28
BspFNI CGCG 2 cut(s) 287, 406
BspLI GGNNCC 3 cut(s) 99, 228, 276
BspOI GCTAGC 1 cut(s) 51
BspPI GGATC 1 cut(s) 127
BspT107I GGYRCC 1 cut(s) 97
BsrBI CCGCTC 1 cut(s) 351
BssMI GATC 1 cut(s) 119
BssNI GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 1 cut(s) 260
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
BstC8I GCNNGC 4 cut(s) 49, 367, 371, 565
BstDEI CTNAG 1 cut(s) 36
BstFNI CGCG 2 cut(s) 287, 406
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 1 cut(s) 122
BstMAI GTCTC 4 cut(s) 98, 258, 301, 383
BstMBI GATC 1 cut(s) 119
BstMCI CGRYCG 2 cut(s) 285, 417
BstMWI GCNNNNNNNGC 3 cut(s) 233, 379, 425
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 455
BstUI CGCG 2 cut(s) 287, 406
BstV1I GCAGC 1 cut(s) 406
BstZI CGGCCG 1 cut(s) 282
BsuRI GGCC 2 cut(s) 57, 284
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 2 cut(s) 243, 265
Cac8I GCNNGC 4 cut(s) 49, 367, 371, 565
CfoI GCGC 1 cut(s) 100
CseI GACGC 3 cut(s) 235, 275, 551
Csp6I GTAC 1 cut(s) 493
CviJI RGCY 9 cut(s) 40, 47, 57, 227, 284, 373, 382, 469, 528
CviKI_1 RGCY 9 cut(s) 40, 47, 57, 227, 284, 373, 382, 469, 528
CviQI GTAC 1 cut(s) 493
DdeI CTNAG 1 cut(s) 36
DinI GGCGCC 1 cut(s) 99
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
DriI GACNNNNNGTC 2 cut(s) 400, 547
EaeI YGGCCR 1 cut(s) 282
EagI CGGCCG 1 cut(s) 282
Eam1104I CTCTTC 1 cut(s) 123
Eam1105I GACNNNNNGTC 2 cut(s) 400, 547
EarI CTCTTC 1 cut(s) 123
Ecl136II GAGCTC 1 cut(s) 373
EclXI CGGCCG 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 375
Eco52I CGGCCG 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 373
Eco88I CYCGRG 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 375
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
Esp3I CGTCTC 4 cut(s) 98, 258, 301, 383
FaiI YATR 3 cut(s) 27, 306, 308
FblI GTMKAC 1 cut(s) 485
Fnu4HI GCNGC 4 cut(s) 285, 352, 420, 526
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 4 cut(s) 285, 352, 420, 526
FspBI CTAG 1 cut(s) 48
GlaI GCGC 1 cut(s) 99
GluI GCNGC 4 cut(s) 285, 352, 420, 526
GsaI CCCAGC 1 cut(s) 97
GsuI CTGGAG 1 cut(s) 407
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 2 cut(s) 57, 284
HapII CCGG 5 cut(s) 101, 185, 233, 281, 425
HgaI GACGC 3 cut(s) 235, 275, 551
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 2 cut(s) 359, 548
HpaII CCGG 5 cut(s) 101, 185, 233, 281, 425
HphI GGTGA 3 cut(s) 20, 202, 568
Hpy166II GTNNAC 4 cut(s) 193, 241, 486, 571
Hpy188I TCNGA 4 cut(s) 37, 124, 445, 541
Hpy188III TCNNGA 4 cut(s) 376, 386, 475, 499
Hpy8I GTNNAC 4 cut(s) 193, 241, 486, 571
HpyAV CCTTC 2 cut(s) 47, 82
HpyCH4III ACNGT 1 cut(s) 260
HpyCH4IV ACGT 5 cut(s) 147, 171, 198, 294, 437
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 379, 425
HpyF3I CTNAG 1 cut(s) 36
HpySE526I ACGT 5 cut(s) 147, 171, 198, 294, 437
Hsp92I GRCGYC 6 cut(s) 98, 147, 198, 246, 294, 543
HspAI GCGC 1 cut(s) 98
KasI GGCGCC 1 cut(s) 97
Kzo9I GATC 1 cut(s) 119
LmnI GCTCC 3 cut(s) 232, 427, 445
Lsp1109I GCAGC 1 cut(s) 406
MaeI CTAG 1 cut(s) 48
MaeII ACGT 5 cut(s) 147, 171, 198, 294, 437
MaeIII GTNAC 2 cut(s) 143, 178
MalI GATC 1 cut(s) 121
MbiI CCGCTC 1 cut(s) 351
MboI GATC 1 cut(s) 119
MboII GAAGA 3 cut(s) 70, 140, 145
MfeI CAATTG 1 cut(s) 339
MhlI GDGCHC 2 cut(s) 375, 450
MluCI AATT 3 cut(s) 11, 310, 339
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 2 cut(s) 368, 557
MnlI CCTC 9 cut(s) 71, 107, 110, 118, 158, 240, 288, 372, 508
MroXI GAANNNNTTC 1 cut(s) 470
MseI TTAA 1 cut(s) 584
MspI CCGG 5 cut(s) 101, 185, 233, 281, 425
MspR9I CCNGG 1 cut(s) 457
MunI CAATTG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 2 cut(s) 287, 406
MwoI GCNNNNNNNGC 3 cut(s) 233, 379, 425
NarI GGCGCC 1 cut(s) 98
NdeII GATC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 47
NlaIV GGNNCC 3 cut(s) 99, 228, 276
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 143
PaeR7I CTCGAG 1 cut(s) 376
PcsI WCGNNNNNNNCGW 3 cut(s) 449, 497, 560
PdmI GAANNNNTTC 1 cut(s) 470
PflFI GACNNNGTC 2 cut(s) 149, 481
PkrI GCNGC 4 cut(s) 286, 353, 421, 527
PleI GAGTC 2 cut(s) 367, 556
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 2 cut(s) 367, 556
Psp124BI GAGCTC 1 cut(s) 375
Psp6I CCWGG 1 cut(s) 455
PspFI CCCAGC 1 cut(s) 93
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 99, 228, 276
PsyI GACNNNGTC 2 cut(s) 149, 481
RsaI GTAC 1 cut(s) 494
RsaNI GTAC 1 cut(s) 493
SacI GAGCTC 1 cut(s) 375
SalI GTCGAC 1 cut(s) 484
SaqAI TTAA 1 cut(s) 584
SatI GCNGC 4 cut(s) 285, 352, 420, 526
Sau3AI GATC 1 cut(s) 119
SchI GAGTC 2 cut(s) 368, 557
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 375, 450
SfoI GGCGCC 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 376
SlaI CTCGAG 1 cut(s) 376
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 3 cut(s) 11, 310, 339
SsiI CCGC 9 cut(s) 158, 188, 206, 254, 285, 344, 351, 406, 525
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 1 cut(s) 48
SstI GAGCTC 1 cut(s) 375
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 1 cut(s) 260
TaiI ACGT 5 cut(s) 150, 174, 201, 297, 440
TaqI TCGA 2 cut(s) 377, 485
TaqII GACCGA 1 cut(s) 403
TasI AATT 3 cut(s) 11, 310, 339
TauI GCSGC 3 cut(s) 287, 354, 528
Tru1I TTAA 1 cut(s) 584
Tru9I TTAA 1 cut(s) 584
TscAI CASTG 2 cut(s) 243, 265
TseFI GTSAC 1 cut(s) 143
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 17, 64, 70
TspGWI ACGGA 1 cut(s) 407
TspRI CASTG 2 cut(s) 243, 265
Tth111I GACNNNGTC 2 cut(s) 149, 481
XhoI CTCGAG 1 cut(s) 376
XmiI GTMKAC 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 470
XspI CTAG 1 cut(s) 48
ZraI GACGTC 3 cut(s) 148, 199, 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.