Rmu_sc0005227.1_g000007

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005227.1
OG0013232
Physical Location & Seq
Reverse (-)
32851 .. 33639
789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005227.1_g000007.1.cds

Sequence Viewer

Length: 789 bp
atggaggacatgcggaagaggcccggagctgacgtcacgaccgacgacgcgtcgtcctcatcctccgcgaagaggcggcgagatcaggccgaaggaggcggtggcatttcggttgttcctccggtagcgaaggacgtcgtcgttgcggacagaggcgacgtttcggttactccggcggtgaacgacgtcgttgcggacaacggcgacattttggctcctccggcagtgaacggcgtcgttgcggacagtggagacgcttcggttcctccggccgcgaacgacgtcgttgtggaaaacggcgacatttcggttcctccggcaatgaacggagtcgttgcggttcctccggcagtgaacgacgtcattgcggagagcggcgacgagtcggcgagcgagctctcgaggcttctggagacggactacctgtcgcggaactcggtcgctgctccggcgagattgacgttctcggagcacccgtccaggtcgccgaagctgttccagacgatgtcgacttcgtacatcacgataaacgggaacgaggagagttgcggctcgtcgttctcggacgccgagtcgtcggtgatggcgagcgtggacaggacagggcgcgacgtggagcaggtgaggctggactacgagtggtacgcggcggagagatgggcggaggggcacggcgagtcgggattcgacgaccgcgcgtgggatgctgccgatcagtcggcgcgtgaaagaatggtgctcaacttggacttgaaacttcaaaaccaaagctttcggtttcaaaactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

27.82

Weight (kDa)

4.33

Isoelectric Point (pI)

52.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013232)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56880
fragaria_vesca FvH4_1g07430 FvH4_1g21692
malus_domestica MD02G1079500.v1.1 MD15G1207000.v1.1
prunus_persica Prupe.7G209700_v2.0.a1
pyrus_communis pycom02g06330
rosa_laevigata RLG00000016392
rosa_multiflora Rmu_sc0005227.1_g000006 Rmu_sc0005227.1_g000007 Rmu_sc0005227.1_g000008
rosa_roxburghii Rroxscaffold_2G00148170
rosa_rugosa Rorug02G0034900
rosa_samantha Rh2AG081100 Rh2BG082200 Rh2CG084400 Rh2DG080100
rosa_wichuraiana Rw2G006560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 610
AatII GACGTC 5 cut(s) 36, 138, 189, 285, 363
Acc36I ACCTGC 1 cut(s) 610
AccBSI CCGCTC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 509
AccII CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
AcoI YGGCCR 1 cut(s) 270
AcyI GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
AfaI GTAC 2 cut(s) 518, 644
AfiI CCNNNNNNNGG 2 cut(s) 72, 700
AflIII ACRYGT 1 cut(s) 48
AgsI TTSAA 3 cut(s) 754, 761, 782
AhdI GACNNNNNGTC 4 cut(s) 49, 52, 424, 571
AjiI CACGTC 1 cut(s) 613
AjnI CCWGG 1 cut(s) 479
AluBI AGCT 4 cut(s) 29, 397, 493, 771
AluI AGCT 4 cut(s) 29, 397, 493, 771
Alw21I GWGCWC 3 cut(s) 399, 474, 741
Alw26I GTCTC 2 cut(s) 246, 407
Ama87I CYCGRG 1 cut(s) 400
AoxI GGCC 3 cut(s) 20, 87, 270
ApeKI GCWGC 2 cut(s) 443, 707
Asp700I GAANNNNTTC 1 cut(s) 494
AspLEI GCGC 3 cut(s) 609, 698, 724
AspS9I GGNCC 1 cut(s) 21
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 3 cut(s) 190, 592, 634
AvaI CYCGRG 1 cut(s) 400
BaeGI GKGCMC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 399
Bbv12I GWGCWC 3 cut(s) 399, 474, 741
BbvI GCAGC 2 cut(s) 430, 694
BccI CCATC 2 cut(s) 577, 651
BceAI ACGGC 4 cut(s) 217, 247, 313, 688
BcgI CGANNNNNNTGC 4 cut(s) 173, 207, 347, 381
BciT130I CCWGG 1 cut(s) 481
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 246, 407
BfuAI ACCTGC 1 cut(s) 610
BisI GCNGC 7 cut(s) 77, 273, 376, 444, 550, 648, 708
BlsI GCNGC 7 cut(s) 78, 274, 377, 445, 551, 649, 709
Bme1390I CCNGG 2 cut(s) 24, 481
BmeRI GACNNNNNGTC 4 cut(s) 49, 52, 424, 571
BmeT110I CYCGRG 1 cut(s) 400
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 1 cut(s) 21
BmiI GGNNCC 4 cut(s) 216, 264, 312, 342
BmrFI CCNGG 2 cut(s) 24, 481
BmsI GCATC 1 cut(s) 694
BpmI CTGGAG 1 cut(s) 431
BpuMI CCSGG 1 cut(s) 24
BsaHI GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 461, 491
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 700
Bse3DI GCAATG 2 cut(s) 327, 363
BseBI CCWGG 1 cut(s) 481
BseGI GGATG 2 cut(s) 59, 709
BseLI CCNNNNNNNGG 2 cut(s) 72, 700
BseMI GCAATG 2 cut(s) 327, 363
BseRI GAGGAG 2 cut(s) 207, 554
BseSI GKGCMC 1 cut(s) 672
BseX3I CGGCCG 1 cut(s) 270
BseXI GCAGC 2 cut(s) 430, 694
Bsh1236I CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
Bsh1285I CGRYCG 4 cut(s) 42, 273, 441, 694
BshFI GGCC 3 cut(s) 22, 89, 272
BsiEI CGRYCG 4 cut(s) 42, 273, 441, 694
BsiHKAI GWGCWC 3 cut(s) 399, 474, 741
BsiHKCI CYCGRG 1 cut(s) 400
BsiSI CCGG 8 cut(s) 24, 122, 173, 221, 269, 317, 347, 449
BslI CCNNNNNNNGG 2 cut(s) 72, 700
BsmAI GTCTC 2 cut(s) 246, 407
BsmBI CGTCTC 2 cut(s) 246, 407
BsnI GGCC 3 cut(s) 22, 89, 272
BsoBI CYCGRG 1 cut(s) 400
Bsp1286I GDGCHC 4 cut(s) 399, 474, 672, 741
Bsp143I GATC 2 cut(s) 82, 712
BspANI GGCC 3 cut(s) 22, 89, 272
BspFNI CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
BspLI GGNNCC 4 cut(s) 216, 264, 312, 342
BspMI ACCTGC 1 cut(s) 610
BsrBI CCGCTC 1 cut(s) 375
BsrDI GCAATG 2 cut(s) 327, 363
BssMI GATC 2 cut(s) 82, 712
BssNI GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
Bst2UI CCWGG 1 cut(s) 481
Bst4CI ACNGT 1 cut(s) 248
Bst6I CTCTTC 2 cut(s) 11, 65
BstACI GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
BstC8I GCNNGC 3 cut(s) 391, 395, 589
BstF5I GGATG 2 cut(s) 59, 709
BstFNI CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
BstHHI GCGC 3 cut(s) 609, 698, 724
BstKTI GATC 2 cut(s) 85, 715
BstMAI GTCTC 2 cut(s) 246, 407
BstMBI GATC 2 cut(s) 82, 712
BstMCI CGRYCG 4 cut(s) 42, 273, 441, 694
BstMWI GCNNNNNNNGC 6 cut(s) 19, 221, 403, 449, 625, 704
BstNI CCWGG 1 cut(s) 481
BstNSI RCATGY 1 cut(s) 13
BstSCI CCNGG 2 cut(s) 22, 479
BstSLI GKGCMC 1 cut(s) 672
BstUI CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
BstV1I GCAGC 2 cut(s) 430, 694
BstZI CGGCCG 1 cut(s) 270
BsuRI GGCC 3 cut(s) 22, 89, 272
BtrI CACGTC 1 cut(s) 613
BtsCI GGATG 2 cut(s) 59, 709
BtsI GCAGTG 2 cut(s) 231, 357
BtsIMutI CAGTG 3 cut(s) 231, 253, 357
BveI ACCTGC 1 cut(s) 610
Cac8I GCNNGC 3 cut(s) 391, 395, 589
CfoI GCGC 3 cut(s) 609, 698, 724
Cfr13I GGNCC 1 cut(s) 21
CseI GACGC 5 cut(s) 39, 56, 223, 263, 575
Csp6I GTAC 2 cut(s) 517, 643
CviAII CATG 1 cut(s) 10
CviQI GTAC 2 cut(s) 517, 643
DpnI GATC 2 cut(s) 84, 714
DpnII GATC 2 cut(s) 82, 712
DriI GACNNNNNGTC 4 cut(s) 49, 52, 424, 571
EaeI YGGCCR 1 cut(s) 270
EagI CGGCCG 1 cut(s) 270
Eam1104I CTCTTC 2 cut(s) 11, 65
Eam1105I GACNNNNNGTC 4 cut(s) 49, 52, 424, 571
EarI CTCTTC 2 cut(s) 11, 65
EciI GGCGGA 2 cut(s) 665, 677
Ecl136II GAGCTC 1 cut(s) 397
EclXI CGGCCG 1 cut(s) 270
Eco24I GRGCYC 1 cut(s) 399
Eco52I CGGCCG 1 cut(s) 270
Eco53kI GAGCTC 1 cut(s) 397
Eco88I CYCGRG 1 cut(s) 400
EcoICRI GAGCTC 1 cut(s) 397
EcoRII CCWGG 1 cut(s) 479
EcoT38I GRGCYC 1 cut(s) 399
Esp3I CGTCTC 2 cut(s) 246, 407
FaeI CATG 1 cut(s) 13
FaiI YATR 1 cut(s) 11
FatI CATG 1 cut(s) 9
FblI GTMKAC 1 cut(s) 509
Fnu4HI GCNGC 7 cut(s) 77, 273, 376, 444, 550, 648, 708
FokI GGATG 2 cut(s) 46, 716
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 7 cut(s) 77, 273, 376, 444, 550, 648, 708
GlaI GCGC 3 cut(s) 608, 697, 723
GluI GCNGC 7 cut(s) 77, 273, 376, 444, 550, 648, 708
GsuI CTGGAG 1 cut(s) 431
HaeIII GGCC 3 cut(s) 22, 89, 272
HapII CCGG 8 cut(s) 24, 122, 173, 221, 269, 317, 347, 449
HgaI GACGC 5 cut(s) 39, 56, 223, 263, 575
HhaI GCGC 3 cut(s) 609, 698, 724
Hin1I GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
Hin1II CATG 1 cut(s) 13
Hin6I GCGC 3 cut(s) 607, 696, 722
HinP1I GCGC 3 cut(s) 607, 696, 722
HincII GTYRAC 1 cut(s) 510
HindII GTYRAC 1 cut(s) 510
HindIII AAGCTT 1 cut(s) 769
HinfI GANTC 5 cut(s) 330, 383, 572, 677, 684
HpaII CCGG 8 cut(s) 24, 122, 173, 221, 269, 317, 347, 449
HphI GGTGA 3 cut(s) 190, 592, 634
Hpy166II GTNNAC 5 cut(s) 181, 229, 355, 510, 595
Hpy188I TCNGA 2 cut(s) 469, 565
Hpy188III TCNNGA 6 cut(s) 37, 400, 410, 499, 523, 681
Hpy8I GTNNAC 5 cut(s) 181, 229, 355, 510, 595
HpyAV CCTTC 2 cut(s) 86, 124
HpyCH4III ACNGT 1 cut(s) 248
HpyCH4IV ACGT 8 cut(s) 33, 135, 159, 186, 282, 360, 461, 612
HpyF10VI GCNNNNNNNGC 6 cut(s) 19, 221, 403, 449, 625, 704
HpySE526I ACGT 8 cut(s) 33, 135, 159, 186, 282, 360, 461, 612
Hsp92I GRCGYC 7 cut(s) 33, 135, 186, 234, 282, 360, 567
Hsp92II CATG 1 cut(s) 13
HspAI GCGC 3 cut(s) 607, 696, 722
Kzo9I GATC 2 cut(s) 82, 712
LmnI GCTCC 5 cut(s) 26, 220, 451, 469, 616
Lsp1109I GCAGC 2 cut(s) 430, 694
LweI GCATC 1 cut(s) 694
MaeII ACGT 8 cut(s) 33, 135, 159, 186, 282, 360, 461, 612
MaeIII GTNAC 2 cut(s) 34, 166
MalI GATC 2 cut(s) 84, 714
MbiI CCGCTC 1 cut(s) 375
MboI GATC 2 cut(s) 82, 712
MboII GAAGA 2 cut(s) 28, 82
MhlI GDGCHC 4 cut(s) 399, 474, 672, 741
MluI ACGCGT 1 cut(s) 48
MlyI GAGTC 4 cut(s) 339, 392, 581, 686
MroXI GAANNNNTTC 1 cut(s) 494
MspI CCGG 8 cut(s) 24, 122, 173, 221, 269, 317, 347, 449
MspR9I CCNGG 2 cut(s) 24, 481
MvaI CCWGG 1 cut(s) 481
MvnI CGCG 9 cut(s) 50, 68, 275, 430, 609, 647, 696, 698, 724
MwoI GCNNNNNNNGC 6 cut(s) 19, 221, 403, 449, 625, 704
NciI CCSGG 1 cut(s) 24
NdeII GATC 2 cut(s) 82, 712
NlaIII CATG 1 cut(s) 13
NlaIV GGNNCC 4 cut(s) 216, 264, 312, 342
NmeAIII GCCGAG 1 cut(s) 595
NmuCI GTSAC 1 cut(s) 34
NspI RCATGY 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 400
PaqCI CACCTGC 1 cut(s) 610
PcsI WCGNNNNNNNCGW 4 cut(s) 39, 473, 521, 584
PdmI GAANNNNTTC 1 cut(s) 494
PfeI GAWTC 1 cut(s) 684
PflFI GACNNNGTC 2 cut(s) 137, 505
PkrI GCNGC 7 cut(s) 78, 274, 377, 445, 551, 649, 709
PleI GAGTC 4 cut(s) 338, 391, 580, 685
PpsI GAGTC 4 cut(s) 338, 391, 580, 685
Psp124BI GAGCTC 1 cut(s) 399
Psp6I CCWGG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 479
PspN4I GGNNCC 4 cut(s) 216, 264, 312, 342
PspPI GGNCC 1 cut(s) 21
PsyI GACNNNGTC 2 cut(s) 137, 505
RsaI GTAC 2 cut(s) 518, 644
RsaNI GTAC 2 cut(s) 517, 643
SacI GAGCTC 1 cut(s) 399
SalI GTCGAC 1 cut(s) 508
SatI GCNGC 7 cut(s) 77, 273, 376, 444, 550, 648, 708
Sau3AI GATC 2 cut(s) 82, 712
Sau96I GGNCC 1 cut(s) 21
SchI GAGTC 4 cut(s) 339, 392, 581, 686
ScrFI CCNGG 2 cut(s) 24, 481
SduI GDGCHC 4 cut(s) 399, 474, 672, 741
SfaNI GCATC 1 cut(s) 694
Sfr274I CTCGAG 1 cut(s) 400
SlaI CTCGAG 1 cut(s) 400
SmlI CTYRAG 1 cut(s) 400
SmoI CTYRAG 1 cut(s) 400
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 2 cut(s) 22, 479
TaaI ACNGT 1 cut(s) 248
TaiI ACGT 8 cut(s) 36, 138, 162, 189, 285, 363, 464, 615
TaqI TCGA 3 cut(s) 401, 509, 687
TaqII GACCGA 2 cut(s) 56, 427
TauI GCSGC 5 cut(s) 79, 275, 378, 552, 650
TfiI GAWTC 1 cut(s) 684
TscAI CASTG 3 cut(s) 231, 253, 357
TseFI GTSAC 1 cut(s) 34
TseI GCWGC 2 cut(s) 443, 707
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 338
TspGWI ACGGA 2 cut(s) 342, 431
TspRI CASTG 3 cut(s) 231, 253, 357
Tth111I GACNNNGTC 2 cut(s) 137, 505
XceI RCATGY 1 cut(s) 13
XhoI CTCGAG 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 509
XmnI GAANNNNTTC 1 cut(s) 494
ZraI GACGTC 5 cut(s) 34, 136, 187, 283, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.