MD15G1212400.v1.1

LYAR-type C2HC zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013475
Physical Location & Seq
Reverse (-)
17059684 .. 17061787
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1212400.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGTGTGGTTTCAGTGCGAGGATTGCGGAGACAACCTCAAGAAACCCAAGGTTTTGGGTCACTTTAACTGCTGCTCTGCTCGAAAGCTTTCGTGCATTGATTGCGGAGAGACATTTGGGAGAGAAGCCGTTCAGGGTCACACACAATGTATGACTGAGGCGGAAAAATACGGTCCCAAGGGGGCACAAGGGAAACCTTTGAATGGTGGGGGAGCCAAGCCCAACAACAACAGGGATGGAAAGCAGCAACCCGATTTTGATATTACTGTCGGGCTGACTAAGCGATTTCCATGGTTTTGTAGTCTTTGCAATACTAAAGCCACTAGTGAGCAGACCTTGCTGCTGCATGCTGAAGGAAAGAAGCACAGGGCAAAAGCCCGAGCCATCCATGCAGCAAAGCAGCAGCCTAAGCAGCCGGAAGAATCTGTTCCGGATACAGAACCTCCACCCAAGGACACTCCCAAGGCTGAAGCTCTTGAGAATAAACAAATAGAAGAACCTAAATCGCATGATGCATCTAGAGTCAATACCGAACAACAAAGTCCTGAAGCAGAGACTTACACCTTGCTGAAAAATAAAAAGAGAAAGCTTGATGCATCCAAGAACGATGAATCAAGCAAGAAGACTAAGGATAATACATCAGATGAAGTAGGAAATGGCAAAGTGGTTCAGAGTGGGAAGACAGAAGCAAATGGGAAAGAGGGAGAACTTACTACGAAAACAAAGTGGAAGAAGTTGATTACGTCAACCCTAAAATCGAACGACGGAGTCCTGAAGATGAGGAAATTGAAAAAACTTGTGCTGAAGGCTCTCGAAGAATCCGGCATAACAGATGACGAAACCAAACTGAGTAAGAAGCTTGAGCACAAGATCAATTCGAGCTCCAAATTTAGAGTCGACGACAAGTATGTTCATTTGGCTGCCAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-LYAR zf-met

Protein Analysis

311

Amino Acids

34.4

Weight (kDa)

9.17

Isoelectric Point (pI)

32.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LYAR PF08790 30 - 57 7.4e-14 LYAR-type C2HC zinc finger
zf-met PF12874 98 - 122 7.3e-07 Zinc-finger of C2H2 type
WHD_LYAR PF25879 239 - 309 1.5e-24 LYAR family winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013475)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19385
fragaria_vesca FvH4_1g08060
malus_domestica MD02G1085300.v1.1 MD15G1212400.v1.1
prunus_persica Prupe.7G204300_v2.0.a1 Prupe.7G204300_v2.0.a1
pyrus_communis pycom02g06810 pycom15g18870
rosa_chinensis RchiOBHm_Chr2g0094111
rosa_laevigata RLG00000016456
rosa_multiflora Rmu_sc0026373.1_g000002 Rmu_ssc0000149.1_g000002
rosa_roxburghii Rroxscaffold_2G00147470
rosa_rugosa Rorug02G0041000
rosa_samantha Rh2AG088600 Rh2BG088700 Rh2CG091100 Rh2DG087700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 897
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 3 cut(s) 27, 105, 161
AcsI RAATTY 1 cut(s) 887
AcuI CTGAAG 5 cut(s) 372, 489, 567, 794, 824
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 2 cut(s) 202, 790
AhlI ACTAGT 1 cut(s) 323
AluBI AGCT 5 cut(s) 88, 473, 589, 859, 882
AluI AGCT 5 cut(s) 88, 473, 589, 859, 882
Alw21I GWGCWC 2 cut(s) 867, 884
Alw26I GTCTC 3 cut(s) 24, 104, 548
Ama87I CYCGRG 1 cut(s) 378
Aor13HI TCCGGA 1 cut(s) 430
ApeKI GCWGC 9 cut(s) 72, 244, 340, 343, 392, 400, 403, 412, 920
ApoI RAATTY 1 cut(s) 887
ArsI GACNNNNNNTTYG 2 cut(s) 879, 911
Asp700I GAANNNNTTC 3 cut(s) 88, 129, 426
AspS9I GGNCC 1 cut(s) 173
AvaI CYCGRG 1 cut(s) 378
AvaII GGWCC 1 cut(s) 173
BaeGI GKGCMC 1 cut(s) 187
BanII GRGCYC 1 cut(s) 884
BbsI GAAGAC 2 cut(s) 629, 686
Bbv12I GWGCWC 2 cut(s) 867, 884
BbvI GCAGC 9 cut(s) 59, 256, 327, 330, 404, 412, 415, 424, 907
BccI CCATC 2 cut(s) 230, 392
BceAI ACGGC 1 cut(s) 113
BciVI GTATCC 1 cut(s) 427
BcoDI GTCTC 3 cut(s) 24, 104, 548
BcuI ACTAGT 1 cut(s) 323
BfaI CTAG 2 cut(s) 324, 519
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 9 cut(s) 73, 245, 341, 344, 393, 401, 404, 413, 921
BlsI GCNGC 9 cut(s) 74, 246, 342, 345, 394, 402, 405, 414, 922
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 378
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 175, 214
BmsI GCATC 4 cut(s) 502, 524, 583, 605
BpiI GAAGAC 2 cut(s) 629, 686
BplI GAGNNNNNCTC 2 cut(s) 21, 53
Bpu10I CCTNAGC 1 cut(s) 408
BpuEI CTTGAG 3 cut(s) 23, 497, 881
BsaJI CCNNGG 6 cut(s) 48, 177, 290, 450, 462, 924
BsaWI WCCGGW 1 cut(s) 430
BsaXI ACNNNNNCTCC 2 cut(s) 427, 457
Bsc4I CCNNNNNNNGG 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 430
BseDI CCNNGG 6 cut(s) 48, 177, 290, 450, 462, 924
BseGI GGATG 3 cut(s) 241, 384, 596
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 2 cut(s) 147, 839
BseSI GKGCMC 1 cut(s) 187
BseXI GCAGC 9 cut(s) 59, 256, 327, 330, 404, 412, 415, 424, 907
BsiHKAI GWGCWC 2 cut(s) 867, 884
BsiHKCI CYCGRG 1 cut(s) 378
BsiSI CCGG 3 cut(s) 416, 431, 822
BslFI GGGAC 1 cut(s) 159
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 3 cut(s) 24, 104, 548
BsmFI GGGAC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 187, 867, 884
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 1 cut(s) 870
Bsp19I CCATGG 1 cut(s) 290
BspACI CCGC 3 cut(s) 27, 105, 161
BspCNI CTCAG 2 cut(s) 148, 840
BspEI TCCGGA 1 cut(s) 430
BspLI GGNNCC 2 cut(s) 175, 214
BssECI CCNNGG 6 cut(s) 48, 177, 290, 450, 462, 924
BssMI GATC 1 cut(s) 870
BssT1I CCWWGG 6 cut(s) 48, 177, 290, 450, 462, 924
Bst4CI ACNGT 2 cut(s) 173, 268
BstAPI GCANNNNNTGC 2 cut(s) 102, 337
BstC8I GCNNGC 1 cut(s) 348
BstDEI CTNAG 5 cut(s) 156, 279, 408, 627, 848
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 3 cut(s) 241, 384, 596
BstKTI GATC 1 cut(s) 873
BstMAI GTCTC 3 cut(s) 24, 104, 548
BstMBI GATC 1 cut(s) 870
BstMWI GCNNNNNNNGC 7 cut(s) 24, 102, 280, 337, 389, 409, 412
BstNSI RCATGY 1 cut(s) 350
BstSLI GKGCMC 1 cut(s) 187
BstV1I GCAGC 9 cut(s) 59, 256, 327, 330, 404, 412, 415, 424, 907
BstV2I GAAGAC 2 cut(s) 629, 686
BstXI CCANNNNNNTGG 1 cut(s) 55
BsuI GTATCC 1 cut(s) 427
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 3 cut(s) 241, 384, 596
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 348
Cfr13I GGNCC 1 cut(s) 173
CviAII CATG 4 cut(s) 291, 347, 389, 509
DdeI CTNAG 5 cut(s) 156, 279, 408, 627, 848
DpnI GATC 1 cut(s) 872
DpnII GATC 1 cut(s) 870
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 882
Eco130I CCWWGG 6 cut(s) 48, 177, 290, 450, 462, 924
Eco24I GRGCYC 1 cut(s) 884
Eco47I GGWCC 1 cut(s) 173
Eco53kI GAGCTC 1 cut(s) 882
Eco57I CTGAAG 5 cut(s) 372, 489, 567, 794, 824
Eco88I CYCGRG 1 cut(s) 378
EcoICRI GAGCTC 1 cut(s) 882
EcoT14I CCWWGG 6 cut(s) 48, 177, 290, 450, 462, 924
EcoT22I ATGCAT 2 cut(s) 517, 598
EcoT38I GRGCYC 1 cut(s) 884
ErhI CCWWGG 6 cut(s) 48, 177, 290, 450, 462, 924
FaeI CATG 4 cut(s) 294, 350, 392, 512
FaiI YATR 7 cut(s) 152, 292, 348, 390, 510, 827, 909
FaqI GGGAC 1 cut(s) 159
FatI CATG 4 cut(s) 290, 346, 388, 508
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 9 cut(s) 73, 245, 341, 344, 393, 401, 404, 413, 921
FokI GGATG 3 cut(s) 248, 371, 583
FriOI GRGCYC 1 cut(s) 884
Fsp4HI GCNGC 9 cut(s) 73, 245, 341, 344, 393, 401, 404, 413, 921
FspBI CTAG 2 cut(s) 324, 519
GluI GCNGC 9 cut(s) 73, 245, 341, 344, 393, 401, 404, 413, 921
HapII CCGG 3 cut(s) 416, 431, 822
Hin1II CATG 4 cut(s) 294, 350, 392, 512
HincII GTYRAC 2 cut(s) 747, 898
HindII GTYRAC 2 cut(s) 747, 898
HindIII AAGCTT 3 cut(s) 86, 587, 857
HinfI GANTC 6 cut(s) 422, 522, 611, 768, 818, 894
HpaII CCGG 3 cut(s) 416, 431, 822
Hpy166II GTNNAC 2 cut(s) 747, 898
Hpy188I TCNGA 2 cut(s) 643, 672
Hpy188III TCNNGA 7 cut(s) 40, 431, 476, 519, 545, 772, 812
Hpy8I GTNNAC 2 cut(s) 747, 898
Hpy99I CGWCG 2 cut(s) 767, 902
HpyAV CCTTC 2 cut(s) 347, 799
HpyCH4III ACNGT 2 cut(s) 173, 268
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 6 cut(s) 96, 309, 346, 392, 515, 596
HpyF10VI GCNNNNNNNGC 7 cut(s) 24, 102, 280, 337, 389, 409, 412
HpyF3I CTNAG 5 cut(s) 156, 279, 408, 627, 848
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 4 cut(s) 294, 350, 392, 512
Kpn2I TCCGGA 1 cut(s) 430
Kzo9I GATC 1 cut(s) 870
LmnI GCTCC 2 cut(s) 212, 887
LpnPI CCDG 8 cut(s) 119, 217, 352, 429, 444, 558, 785, 835
Lsp1109I GCAGC 9 cut(s) 59, 256, 327, 330, 404, 412, 415, 424, 907
LweI GCATC 4 cut(s) 502, 524, 583, 605
MaeI CTAG 2 cut(s) 324, 519
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 2 cut(s) 59, 137
MalI GATC 1 cut(s) 872
MboI GATC 1 cut(s) 870
MboII GAAGA 7 cut(s) 431, 506, 634, 691, 742, 787, 827
MhlI GDGCHC 3 cut(s) 187, 867, 884
MluCI AATT 3 cut(s) 785, 874, 887
MlyI GAGTC 3 cut(s) 531, 777, 903
MnlI CCTC 6 cut(s) 13, 47, 151, 453, 694, 774
Mph1103I ATGCAT 2 cut(s) 517, 598
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 3 cut(s) 88, 129, 426
MseI TTAA 2 cut(s) 66, 931
MspI CCGG 3 cut(s) 416, 431, 822
MwoI GCNNNNNNNGC 7 cut(s) 24, 102, 280, 337, 389, 409, 412
NcoI CCATGG 1 cut(s) 290
NdeII GATC 1 cut(s) 870
NlaIII CATG 4 cut(s) 294, 350, 392, 512
NlaIV GGNNCC 2 cut(s) 175, 214
NmuCI GTSAC 2 cut(s) 59, 137
NsiI ATGCAT 2 cut(s) 517, 598
NspI RCATGY 1 cut(s) 350
PaeI GCATGC 1 cut(s) 350
PdmI GAANNNNTTC 3 cut(s) 88, 129, 426
PfeI GAWTC 3 cut(s) 422, 611, 818
PflFI GACNNNGTC 1 cut(s) 767
PkrI GCNGC 9 cut(s) 74, 246, 342, 345, 394, 402, 405, 414, 922
PleI GAGTC 3 cut(s) 530, 776, 902
PpsI GAGTC 3 cut(s) 530, 776, 902
Psp124BI GAGCTC 1 cut(s) 884
PspN4I GGNNCC 2 cut(s) 175, 214
PspPI GGNCC 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 767
SacI GAGCTC 1 cut(s) 884
SalI GTCGAC 1 cut(s) 896
SaqAI TTAA 2 cut(s) 66, 931
SatI GCNGC 9 cut(s) 73, 245, 341, 344, 393, 401, 404, 413, 921
Sau3AI GATC 1 cut(s) 870
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 3 cut(s) 531, 777, 903
SduI GDGCHC 3 cut(s) 187, 867, 884
SfaNI GCATC 4 cut(s) 502, 524, 583, 605
SinI GGWCC 1 cut(s) 173
SmlI CTYRAG 3 cut(s) 38, 476, 860
SmoI CTYRAG 3 cut(s) 38, 476, 860
SpeI ACTAGT 1 cut(s) 323
SphI GCATGC 1 cut(s) 350
Sse9I AATT 3 cut(s) 785, 874, 887
SsiI CCGC 3 cut(s) 27, 105, 161
SspMI CTAG 2 cut(s) 324, 519
SstI GAGCTC 1 cut(s) 884
StyI CCWWGG 6 cut(s) 48, 177, 290, 450, 462, 924
TaaI ACNGT 2 cut(s) 173, 268
TaiI ACGT 1 cut(s) 746
TaqI TCGA 5 cut(s) 82, 758, 813, 878, 897
TasI AATT 3 cut(s) 785, 874, 887
TfiI GAWTC 3 cut(s) 422, 611, 818
Tru1I TTAA 2 cut(s) 66, 931
Tru9I TTAA 2 cut(s) 66, 931
TscAI CASTG 1 cut(s) 20
TseFI GTSAC 2 cut(s) 59, 137
TseI GCWGC 9 cut(s) 72, 244, 340, 343, 392, 400, 403, 412, 920
Tsp45I GTSAC 2 cut(s) 59, 137
TspDTI ATGAA 3 cut(s) 624, 660, 902
TspGWI ACGGA 1 cut(s) 780
TspRI CASTG 1 cut(s) 20
Tth111I GACNNNGTC 1 cut(s) 767
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 1 cut(s) 887
XbaI TCTAGA 1 cut(s) 518
XceI RCATGY 1 cut(s) 350
XmiI GTMKAC 1 cut(s) 897
XmnI GAANNNNTTC 3 cut(s) 88, 129, 426
XspI CTAG 2 cut(s) 324, 519
Zsp2I ATGCAT 2 cut(s) 517, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.