MD15G1238900.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007936
Physical Location & Seq
Reverse (-)
19674240 .. 19676118
1879 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1238900.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGAAGGAAATGACTCGCCCGATATGTCGACGGTATCGCGTCATCGTTTCGGGGACGACTTGGAAGAACCGGCCTTCTCCGTCTCCGTCATCGAGAACATGAAGGAAGAGTACGGGCTCTTCGTGTGGCCCTGCAGCTTAGTCCTGGCGGAGTATGTTTGGCAACAGAGATTGCGCTTTTCCGGAGCTAGCGTAGTCGAGCTTGGCGCTGGAACTTCCTTGCCCGGTCTGGTTGCCGCCAGAGTTGGTGCTGACGTCACTCTCACTGATGATTCGAGTAGATTGGAGGTGCTGGACAACATGAGAAGAGTGCTTGACCTTAATAAACTTGAGTGCAAGGTAATGGGATTGACATGGGGAGCTTGGGATGCATCAACGTTCAGCTTACACCCCAAATTCATTCTCGGGGCTGATGTATTATATGATGCTACTGCCTTTGATGACCTCTTTGCCACCGTGACATTTCTGCTCCAAAATTCTCCGGGGTCAGTCTTCATAACAACATACCATAATCGAAGCGGGCATCGTCTTATTGAATTCTTGATGGTCAAATGGGGATTGAAGTGCTTGAAGCTTCTTGACGCCTTTTCGTTTATGCCATCCTACAAGGCATCCGGGCTACGTGGCAACCTTCAACTGGCAGAGATCGCTCTGGATAGCGAACAGGCTGAGACAACGGTTTTGCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_16

Protein Analysis

230

Amino Acids

25.44

Weight (kDa)

4.84

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_16 PF10294 29 - 173 5.3e-20 Lysine methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 258
AccI GTMKAC 1 cut(s) 29
AccII CGCG 1 cut(s) 40
AccIII TCCGGA 1 cut(s) 182
AciI CCGC 3 cut(s) 149, 237, 519
AclI AACGTT 1 cut(s) 377
AcsI RAATTY 3 cut(s) 395, 475, 536
AcyI GRCGYC 2 cut(s) 255, 582
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 1 cut(s) 637
AgsI TTSAA 4 cut(s) 536, 562, 571, 635
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 6 cut(s) 138, 188, 202, 362, 384, 574
AluI AGCT 6 cut(s) 138, 188, 202, 362, 384, 574
Alw26I GTCTC 2 cut(s) 88, 665
Ama87I CYCGRG 1 cut(s) 404
Aor13HI TCCGGA 1 cut(s) 182
AoxI GGCC 2 cut(s) 72, 128
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 3 cut(s) 395, 475, 536
ArsI GACNNNNNNTTYG 1 cut(s) 664
AspLEI GCGC 2 cut(s) 177, 209
AspS9I GGNCC 1 cut(s) 129
AsuC2I CCSGG 3 cut(s) 225, 483, 616
AsuNHI GCTAGC 1 cut(s) 188
AvaI CYCGRG 1 cut(s) 404
BanII GRGCYC 1 cut(s) 120
BarI GAAGNNNNNNTAC 4 cut(s) 95, 104, 127, 136
BbsI GAAGAC 1 cut(s) 484
BbvI GCAGC 1 cut(s) 147
BccI CCATC 2 cut(s) 538, 607
BciT130I CCWGG 1 cut(s) 146
BcnI CCSGG 3 cut(s) 225, 483, 616
BcoDI GTCTC 2 cut(s) 88, 665
BfaI CTAG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 133
BfoI RGCGCY 1 cut(s) 210
BisI GCNGC 2 cut(s) 136, 237
BlsI GCNGC 2 cut(s) 137, 238
Bme1390I CCNGG 4 cut(s) 146, 225, 483, 616
BmeT110I CYCGRG 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 129
BmrFI CCNGG 4 cut(s) 146, 225, 483, 616
BmsI GCATC 5 cut(s) 358, 380, 415, 532, 620
BmtI GCTAGC 1 cut(s) 192
BpiI GAAGAC 1 cut(s) 484
BpuEI CTTGAG 1 cut(s) 350
BpuMI CCSGG 3 cut(s) 225, 483, 616
BsaAI YACGTR 1 cut(s) 623
BsaHI GRCGYC 2 cut(s) 255, 582
BsaJI CCNNGG 1 cut(s) 482
BsaWI WCCGGW 1 cut(s) 182
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 1 cut(s) 637
Bse118I RCCGGY 1 cut(s) 70
Bse1I ACTGG 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 182
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 3 cut(s) 373, 599, 611
BseLI CCNNNNNNNGG 1 cut(s) 637
BseMII CTCAG 1 cut(s) 660
BseNI ACTGG 1 cut(s) 642
BseXI GCAGC 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 40
BshFI GGCC 2 cut(s) 74, 130
BsiHKCI CYCGRG 1 cut(s) 404
BsiSI CCGG 5 cut(s) 71, 183, 225, 482, 615
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 637
BsmAI GTCTC 2 cut(s) 88, 665
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 2 cut(s) 74, 130
BsoBI CYCGRG 1 cut(s) 404
Bsp1286I GDGCHC 1 cut(s) 120
Bsp13I TCCGGA 1 cut(s) 182
Bsp143I GATC 1 cut(s) 645
BspACI CCGC 3 cut(s) 149, 237, 519
BspANI GGCC 2 cut(s) 74, 130
BspCNI CTCAG 1 cut(s) 661
BspEI TCCGGA 1 cut(s) 182
BspFNI CGCG 1 cut(s) 40
BspMAI CTGCAG 1 cut(s) 137
BspOI GCTAGC 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 70
BsrI ACTGG 1 cut(s) 642
BssAI RCCGGY 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 1 cut(s) 645
BssNI GRCGYC 2 cut(s) 255, 582
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 34, 457, 679
Bst6I CTCTTC 3 cut(s) 102, 125, 301
BstACI GRCGYC 2 cut(s) 255, 582
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 2 cut(s) 190, 521
BstDEI CTNAG 2 cut(s) 139, 669
BstF5I GGATG 3 cut(s) 373, 599, 611
BstFNI CGCG 1 cut(s) 40
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 2 cut(s) 177, 209
BstKTI GATC 1 cut(s) 648
BstMAI GTCTC 2 cut(s) 88, 665
BstMBI GATC 1 cut(s) 645
BstMWI GCNNNNNNNGC 2 cut(s) 368, 647
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 4 cut(s) 144, 223, 481, 614
BstSFI CTRYAG 1 cut(s) 133
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 1 cut(s) 147
BstV2I GAAGAC 1 cut(s) 484
BsuRI GGCC 2 cut(s) 74, 130
BtsCI GGATG 3 cut(s) 373, 599, 611
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 2 cut(s) 190, 521
CfoI GCGC 2 cut(s) 177, 209
Cfr10I RCCGGY 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 129
CseI GACGC 2 cut(s) 29, 590
Csp6I GTAC 1 cut(s) 112
CviAII CATG 3 cut(s) 100, 301, 354
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 139, 669
DpnI GATC 1 cut(s) 647
DpnII GATC 1 cut(s) 645
Eam1104I CTCTTC 3 cut(s) 102, 125, 301
EarI CTCTTC 3 cut(s) 102, 125, 301
EciI GGCGGA 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 536
EcoRII CCWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 373
EcoT38I GRGCYC 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 3 cut(s) 103, 304, 357
FaqI GGGAC 1 cut(s) 68
FatI CATG 3 cut(s) 99, 300, 353
FauI CCCGC 1 cut(s) 512
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 2 cut(s) 136, 237
FokI GGATG 3 cut(s) 380, 586, 598
FriOI GRGCYC 1 cut(s) 120
Fsp4HI GCNGC 2 cut(s) 136, 237
FspBI CTAG 1 cut(s) 189
GlaI GCGC 2 cut(s) 176, 208
GluI GCNGC 2 cut(s) 136, 237
HaeII RGCGCY 1 cut(s) 210
HaeIII GGCC 2 cut(s) 74, 130
HapII CCGG 5 cut(s) 71, 183, 225, 482, 615
HgaI GACGC 2 cut(s) 29, 590
HhaI GCGC 2 cut(s) 177, 209
Hin1I GRCGYC 2 cut(s) 255, 582
Hin1II CATG 3 cut(s) 103, 304, 357
Hin6I GCGC 2 cut(s) 175, 207
HinP1I GCGC 2 cut(s) 175, 207
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 14, 272
HpaII CCGG 5 cut(s) 71, 183, 225, 482, 615
Hpy166II GTNNAC 1 cut(s) 30
Hpy188III TCNNGA 5 cut(s) 94, 183, 541, 578, 653
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 3 cut(s) 85, 97, 641
HpyCH4III ACNGT 3 cut(s) 34, 457, 679
HpyCH4IV ACGT 3 cut(s) 255, 377, 622
HpyCH4V TGCA 3 cut(s) 135, 336, 371
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 647
HpyF3I CTNAG 2 cut(s) 139, 669
HpySE526I ACGT 3 cut(s) 255, 377, 622
Hsp92I GRCGYC 2 cut(s) 255, 582
Hsp92II CATG 3 cut(s) 103, 304, 357
HspAI GCGC 2 cut(s) 175, 207
Kpn2I TCCGGA 1 cut(s) 182
Kzo9I GATC 1 cut(s) 645
LguI GCTCTTC 1 cut(s) 125
LmnI GCTCC 3 cut(s) 185, 359, 474
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 5 cut(s) 358, 380, 415, 532, 620
MaeI CTAG 1 cut(s) 189
MaeII ACGT 3 cut(s) 255, 377, 622
MaeIII GTNAC 2 cut(s) 256, 457
MalI GATC 1 cut(s) 647
MboI GATC 1 cut(s) 645
MboII GAAGA 5 cut(s) 77, 112, 119, 318, 484
MhlI GDGCHC 1 cut(s) 120
MluCI AATT 3 cut(s) 395, 475, 536
MlyI GAGTC 1 cut(s) 8
MnlI CCTC 2 cut(s) 280, 455
Mph1103I ATGCAT 1 cut(s) 373
MroI TCCGGA 1 cut(s) 182
MseI TTAA 1 cut(s) 321
MspI CCGG 5 cut(s) 71, 183, 225, 482, 615
MspR9I CCNGG 4 cut(s) 146, 225, 483, 616
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 2 cut(s) 368, 647
NciI CCSGG 3 cut(s) 225, 483, 616
NdeII GATC 1 cut(s) 645
NheI GCTAGC 1 cut(s) 188
NlaIII CATG 3 cut(s) 103, 304, 357
NmuCI GTSAC 2 cut(s) 256, 457
NsiI ATGCAT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 125
PcsI WCGNNNNNNNCGW 1 cut(s) 120
PfeI GAWTC 1 cut(s) 272
PkrI GCNGC 2 cut(s) 137, 238
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 623
Psp1406I AACGTT 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspPI GGNCC 1 cut(s) 129
PstI CTGCAG 1 cut(s) 137
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
SalI GTCGAC 1 cut(s) 28
SapI GCTCTTC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 2 cut(s) 136, 237
Sau3AI GATC 1 cut(s) 645
Sau96I GGNCC 1 cut(s) 129
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 4 cut(s) 146, 225, 483, 616
SduI GDGCHC 1 cut(s) 120
SfaNI GCATC 5 cut(s) 358, 380, 415, 532, 620
SfcI CTRYAG 1 cut(s) 133
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
Sse9I AATT 3 cut(s) 395, 475, 536
SsiI CCGC 3 cut(s) 149, 237, 519
SspMI CTAG 1 cut(s) 189
StyD4I CCNGG 4 cut(s) 144, 223, 481, 614
TaaI ACNGT 3 cut(s) 34, 457, 679
TaiI ACGT 3 cut(s) 258, 380, 625
TaqI TCGA 5 cut(s) 29, 93, 198, 275, 514
TasI AATT 3 cut(s) 395, 475, 536
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 1 cut(s) 272
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 256, 457
TseI GCWGC 1 cut(s) 135
Tsp45I GTSAC 2 cut(s) 256, 457
TspDTI ATGAA 3 cut(s) 116, 388, 484
TspGWI ACGGA 2 cut(s) 70, 76
TspRI CASTG 1 cut(s) 271
XapI RAATTY 3 cut(s) 395, 475, 536
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 189
ZraI GACGTC 1 cut(s) 256
Zsp2I ATGCAT 1 cut(s) 373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.