Rorug02G0073900

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003977
Physical Location & Seq
Forward (+)
5878052 .. 5880703
2652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0073900.1

Sequence Viewer

Length: 729 bp
ATGAGCTATGAGCAATACCGCAGATGGATTATCCTCGCCTTTCCTGACGGGGGGCGGGGACCTGTTCTTGATCTTGTTGCAGGATCATTATCTGGAGGAACAGCTGTACTCTTTACCTATCCTCTTGATTTAGTTCGTACTAAGTTGGCTTATCAGGTTGTTGGTTCACCAAAGTTAAAATTGCAAGGGATCATGAATAATGAACAACTTTATAAAGGAATTGTTGATTGTTTCTCTAAGACTTACAAAGAGGCTGGACTTAGAGGCCTCTATCGCGGTGTTGCTCCATCCCTTTATGGAATCTTCCCATATGCCGGTTTGAAGTTCTACTTCTATGAGAAGATGAAGCGTTATGTACCTCCGGAGCAGAAAAGCAGTATTATGGTGAAGCTTGTATGTGGTTCTGTTGCTGGTTTATTAGGTCAGACTTTTACATACCCCCTTGATGTTGTGAGGAGGCAAATGCAGGTTCAACGACTCTTCGCATCAAACAGTCCTGAGATGAAAGGTACAATGGAAAGCCTTGTTTTGATTGCTAAACAGCAAGGATGGAAGCAATTATTTTCAGGACTTAGCATCAATTACTTGAAGGTTGTGCCATCTGTGGCAATTGGATTCACTGTTTACGATGTTATGAAGGCCTACCTTCAAGTTCCATCACGAGACGAAGGTGTGAAAGAAGTGGTAACCAGCAAAACAACTTCCCAGCCATCCTCGATTCACTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

242

Amino Acids

26.97

Weight (kDa)

9.64

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mito_carr PF00153 21 - 119 1e-24 Mitochondrial carrier protein
Mito_carr PF00153 124 - 217 1e-21 Mitochondrial carrier protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 213
Acc36I ACCTGC 1 cut(s) 457
AccII CGCG 1 cut(s) 276
AccIII TCCGGA 1 cut(s) 361
AciI CCGC 3 cut(s) 19, 55, 276
AclWI GGATC 2 cut(s) 91, 197
AfaI GTAC 4 cut(s) 108, 139, 357, 511
AfiI CCNNNNNNNGG 2 cut(s) 50, 314
AgsI TTSAA 4 cut(s) 322, 473, 589, 650
AluBI AGCT 3 cut(s) 6, 104, 391
AluI AGCT 3 cut(s) 6, 104, 391
Alw26I GTCTC 1 cut(s) 657
AlwI GGATC 2 cut(s) 91, 197
Aor13HI TCCGGA 1 cut(s) 361
AoxI GGCC 2 cut(s) 265, 639
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 2 cut(s) 159, 397
AvaII GGWCC 1 cut(s) 59
BauI CACGAG 1 cut(s) 660
BccI CCATC 6 cut(s) 18, 295, 543, 607, 664, 718
BcoDI GTCTC 1 cut(s) 657
BfaI CTAG 1 cut(s) 727
BfuAI ACCTGC 1 cut(s) 457
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 60
BmsI GCATC 2 cut(s) 494, 585
BpmI CTGGAG 1 cut(s) 114
BsaBI GATNNNNATC 1 cut(s) 88
BsaWI WCCGGW 1 cut(s) 361
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 314
Bse118I RCCGGY 1 cut(s) 314
Bse8I GATNNNNATC 1 cut(s) 88
BseAI TCCGGA 1 cut(s) 361
BseGI GGATG 3 cut(s) 287, 554, 710
BseJI GATNNNNATC 1 cut(s) 88
BseLI CCNNNNNNNGG 2 cut(s) 50, 314
BseMII CTCAG 1 cut(s) 489
BseRI GAGGAG 1 cut(s) 469
BseYI CCCAGC 1 cut(s) 705
Bsh1236I CGCG 1 cut(s) 276
BshFI GGCC 2 cut(s) 267, 641
BsiSI CCGG 2 cut(s) 315, 362
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 2 cut(s) 50, 314
BsmAI GTCTC 1 cut(s) 657
BsmBI CGTCTC 1 cut(s) 657
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 2 cut(s) 267, 641
Bsp13I TCCGGA 1 cut(s) 361
Bsp143I GATC 3 cut(s) 70, 83, 189
BspACI CCGC 3 cut(s) 19, 55, 276
BspANI GGCC 2 cut(s) 267, 641
BspCNI CTCAG 1 cut(s) 490
BspEI TCCGGA 1 cut(s) 361
BspFNI CGCG 1 cut(s) 276
BspHI TCATGA 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 60
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 2 cut(s) 91, 197
BsrFI RCCGGY 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 314
BssMI GATC 3 cut(s) 70, 83, 189
BssSI CACGAG 1 cut(s) 660
Bst2BI CACGAG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 494, 622
Bst6I CTCTTC 1 cut(s) 485
BstDEI CTNAG 5 cut(s) 141, 237, 260, 498, 572
BstEII GGTNACC 1 cut(s) 685
BstF5I GGATG 3 cut(s) 287, 554, 710
BstFNI CGCG 1 cut(s) 276
BstKTI GATC 3 cut(s) 73, 86, 192
BstMAI GTCTC 1 cut(s) 657
BstMBI GATC 3 cut(s) 70, 83, 189
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstPI GGTNACC 1 cut(s) 685
BstUI CGCG 1 cut(s) 276
BsuRI GGCC 2 cut(s) 267, 641
BtsCI GGATG 3 cut(s) 287, 554, 710
BtsIMutI CAGTG 1 cut(s) 618
BveI ACCTGC 1 cut(s) 457
CciI TCATGA 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 59
Csp6I GTAC 4 cut(s) 107, 138, 356, 510
CviAII CATG 1 cut(s) 193
CviJI RGCY 9 cut(s) 6, 104, 149, 254, 267, 391, 522, 641, 709
CviKI_1 RGCY 9 cut(s) 6, 104, 149, 254, 267, 391, 522, 641, 709
CviQI GTAC 4 cut(s) 107, 138, 356, 510
DdeI CTNAG 5 cut(s) 141, 237, 260, 498, 572
DpnI GATC 3 cut(s) 72, 85, 191
DpnII GATC 3 cut(s) 70, 83, 189
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Eco147I AGGCCT 2 cut(s) 267, 641
Eco47I GGWCC 1 cut(s) 59
Eco91I GGTNACC 1 cut(s) 685
EcoO109I RGGNCCY 1 cut(s) 59
EcoO65I GGTNACC 1 cut(s) 685
Esp3I CGTCTC 1 cut(s) 657
FaeI CATG 1 cut(s) 196
FalI AAGNNNNNCTT 2 cut(s) 314, 346
FaqI GGGAC 1 cut(s) 72
FatI CATG 1 cut(s) 192
FauI CCCGC 1 cut(s) 48
FauNDI CATATG 1 cut(s) 310
FokI GGATG 3 cut(s) 274, 561, 697
FspBI CTAG 1 cut(s) 727
GsaI CCCAGC 1 cut(s) 709
GsuI CTGGAG 1 cut(s) 114
HaeIII GGCC 2 cut(s) 267, 641
HapII CCGG 2 cut(s) 315, 362
Hin1II CATG 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 4 cut(s) 300, 477, 615, 718
HpaII CCGG 2 cut(s) 315, 362
HphI GGTGA 2 cut(s) 159, 397
Hpy166II GTNNAC 2 cut(s) 167, 625
Hpy188I TCNGA 1 cut(s) 426
Hpy188III TCNNGA 9 cut(s) 44, 68, 93, 125, 193, 362, 497, 567, 660
Hpy8I GTNNAC 2 cut(s) 167, 625
HpyAV CCTTC 4 cut(s) 583, 631, 656, 662
HpyCH4III ACNGT 2 cut(s) 494, 622
HpyCH4V TGCA 3 cut(s) 80, 184, 466
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 5 cut(s) 141, 237, 260, 498, 572
Hsp92II CATG 1 cut(s) 196
Kpn2I TCCGGA 1 cut(s) 361
Kzo9I GATC 3 cut(s) 70, 83, 189
LmnI GCTCC 2 cut(s) 289, 364
LweI GCATC 2 cut(s) 494, 585
MaeI CTAG 1 cut(s) 727
MaeIII GTNAC 1 cut(s) 685
MalI GATC 3 cut(s) 72, 85, 191
MboI GATC 3 cut(s) 70, 83, 189
MboII GAAGA 3 cut(s) 295, 352, 472
MfeI CAATTG 1 cut(s) 609
MluCI AATT 5 cut(s) 179, 219, 557, 580, 609
MlyI GAGTC 1 cut(s) 471
MroI TCCGGA 1 cut(s) 361
MseI TTAA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 315, 362
MunI CAATTG 1 cut(s) 609
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 1 cut(s) 273
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 70, 83, 189
NlaIII CATG 1 cut(s) 196
NlaIV GGNNCC 1 cut(s) 60
PagI TCATGA 1 cut(s) 192
PceI AGGCCT 2 cut(s) 267, 641
PfeI GAWTC 3 cut(s) 300, 615, 718
PleI GAGTC 1 cut(s) 471
PpsI GAGTC 1 cut(s) 471
PpuMI RGGWCCY 1 cut(s) 59
PsiI TTATAA 1 cut(s) 213
Psp5II RGGWCCY 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 685
PspFI CCCAGC 1 cut(s) 705
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 59
PspPPI RGGWCCY 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 4 cut(s) 108, 139, 357, 511
RsaNI GTAC 4 cut(s) 107, 138, 356, 510
SaqAI TTAA 1 cut(s) 176
Sau3AI GATC 3 cut(s) 70, 83, 189
Sau96I GGNCC 1 cut(s) 59
SchI GAGTC 1 cut(s) 471
SfaNI GCATC 2 cut(s) 494, 585
SinI GGWCC 1 cut(s) 59
Sse9I AATT 5 cut(s) 179, 219, 557, 580, 609
SseBI AGGCCT 2 cut(s) 267, 641
SsiI CCGC 3 cut(s) 19, 55, 276
SspMI CTAG 1 cut(s) 727
StuI AGGCCT 2 cut(s) 267, 641
TaaI ACNGT 2 cut(s) 494, 622
TaqI TCGA 1 cut(s) 716
TasI AATT 5 cut(s) 179, 219, 557, 580, 609
TatI WGTACW 1 cut(s) 106
TfiI GAWTC 3 cut(s) 300, 615, 718
Tru1I TTAA 1 cut(s) 176
Tru9I TTAA 1 cut(s) 176
TscAI CASTG 1 cut(s) 625
TspDTI ATGAA 5 cut(s) 209, 216, 359, 518, 650
TspRI CASTG 1 cut(s) 625
VpaK11BI GGWCC 1 cut(s) 59
XspI CTAG 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.