Rw6G019710

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003977
Physical Location & Seq
Forward (+)
39429538 .. 39431486
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G019710.1

Sequence Viewer

Length: 843 bp
ATGACGAAGGAAGTGAGCGAGGAGCCGCAAGCCCACCACCACAAGGACTACGTCGACCCACCGCCGGCGCCGCTCTTCGACATCGACGAGCTTAAGCGCTGGTCCTTCTACAGGGCTCTCATAGCCGAGTTCATCGCCACCCTCCTCTTCCTCTACGTCACCGTCGCCACCGTCATCGGCCACAAGCACCAGACCGGCCCCTGCGACGGCGTCGGCCTTCTCGGCATCGCCTGGGCCTTTGGTGGCATGATCTTTGTCCTCGTTTACTGCACCGCCGGTATCTCTGGTGGTCACATTAACCCGGCGGTGACTTTCGGGCTTTTCTTGGCTCGTAAGGTCTCTCTGATCCGGGCCGTGGCTTACATGGTTGCCCAGTGCTTGGGAGCTATCGCCGGTGTCGCGCTGGTCAAGGCCTTCCAGAGTAGCTACTTCAAGACCCTCGGCGGCGGCACCAACTCTGTCTCTGCTGGCTACAGCAAGGGCACAGCTCTCGGCGCTGAGATCATCGGCACCTTTGTGCTTGTCTACACCGTCTTCTCAGCCACCGACCCCAAGAGGAGCGCGCGTGACTCTCACGTGCCTGTGTTGGCTCCTTTGCCTATTGGGTTTGCTGTGTTTGTTGTGCACTTGGCTACCATCCCCATCACCGGAACTGGCATCAACCCTGCTCGGAGCTTCGGTGCTGCCGTCATTTACAACAAGGAGAAAATCTGGGATGACCAATGGATCTTCTGGGTCGGACCCTTTGTCGGAGCTCTTTGTGCAGCCGCGTACCACCAGTACATCCTGCGAGCCGCCGCCATCAAAGCTTTGGGTTCATTCCGGAGCAACCCCTCCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

280

Amino Acids

29.96

Weight (kDa)

8.99

Isoelectric Point (pI)

35.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 30 - 259 1.2e-82 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 67, 449, 509
AccB7I CCANNNNNTGG 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 73
AccI GTMKAC 2 cut(s) 54, 525
AccII CGCG 4 cut(s) 401, 563, 565, 770
AccIII TCCGGA 1 cut(s) 822
AclWI GGATC 2 cut(s) 340, 734
AcoI YGGCCR 1 cut(s) 178
AcvI CACGTG 1 cut(s) 577
AcyI GRCGYC 2 cut(s) 68, 210
AfaI GTAC 2 cut(s) 773, 782
AfeI AGCGCT 1 cut(s) 98
AfiI CCNNNNNNNGG 8 cut(s) 43, 64, 111, 206, 355, 379, 647, 749
AflII CTTAAG 1 cut(s) 92
AgsI TTSAA 1 cut(s) 433
AhdI GACNNNNNGTC 1 cut(s) 746
AjnI CCWGG 1 cut(s) 230
AleI CACNNNNGTG 1 cut(s) 515
AluBI AGCT 7 cut(s) 91, 386, 426, 488, 675, 755, 809
AluI AGCT 7 cut(s) 91, 386, 426, 488, 675, 755, 809
Alw21I GWGCWC 2 cut(s) 627, 757
Alw26I GTCTC 2 cut(s) 343, 466
Alw44I GTGCAC 1 cut(s) 623
AlwI GGATC 2 cut(s) 340, 734
Aor13HI TCCGGA 1 cut(s) 822
Aor51HI AGCGCT 1 cut(s) 98
AoxI GGCC 6 cut(s) 178, 196, 214, 234, 351, 411
ApaLI GTGCAC 1 cut(s) 623
ApeKI GCWGC 2 cut(s) 683, 764
AspLEI GCGC 6 cut(s) 70, 99, 403, 497, 563, 565
AspS9I GGNCC 5 cut(s) 102, 197, 234, 351, 740
AsuC2I CCSGG 2 cut(s) 302, 350
AsuHPI GGTGA 3 cut(s) 151, 319, 637
AvaII GGWCC 2 cut(s) 102, 740
BaeGI GKGCMC 2 cut(s) 485, 627
BanI GGYRCC 3 cut(s) 67, 449, 509
BanII GRGCYC 2 cut(s) 118, 757
BbrPI CACGTG 1 cut(s) 577
BbsI GAAGAC 1 cut(s) 526
Bbv12I GWGCWC 2 cut(s) 627, 757
BbvI GCAGC 2 cut(s) 670, 776
BccI CCATC 3 cut(s) 644, 650, 809
BceAI ACGGC 3 cut(s) 223, 338, 671
BcgI CGANNNNNNTGC 2 cut(s) 472, 506
BciT130I CCWGG 1 cut(s) 232
BcnI CCSGG 2 cut(s) 302, 350
BcoDI GTCTC 2 cut(s) 343, 466
BfmI CTRYAG 2 cut(s) 109, 472
BfoI RGCGCY 3 cut(s) 71, 100, 498
BfrI CTTAAG 1 cut(s) 92
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 9 cut(s) 26, 71, 445, 448, 684, 765, 768, 795, 798
BlsI GCNGC 9 cut(s) 27, 72, 446, 449, 685, 766, 769, 796, 799
Bme1390I CCNGG 3 cut(s) 232, 302, 350
Bme18I GGWCC 2 cut(s) 102, 740
BmeRI GACNNNNNGTC 1 cut(s) 746
BmgT120I GGNCC 5 cut(s) 102, 197, 234, 351, 740
BmiI GGNNCC 7 cut(s) 24, 69, 199, 451, 511, 591, 742
BmrFI CCNGG 3 cut(s) 232, 302, 350
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 2 cut(s) 234, 666
BmuI ACTGGG 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 526
BpuMI CCSGG 2 cut(s) 302, 350
BsaAI YACGTR 1 cut(s) 577
BsaHI GRCGYC 2 cut(s) 68, 210
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 3 cut(s) 231, 354, 439
BsaWI WCCGGW 2 cut(s) 647, 822
BsaXI ACNNNNNCTCC 4 cut(s) 550, 580, 664, 694
Bsc4I CCNNNNNNNGG 8 cut(s) 43, 64, 111, 206, 355, 379, 647, 749
Bse118I RCCGGY 4 cut(s) 64, 194, 275, 392
Bse1I ACTGG 3 cut(s) 373, 658, 778
BseAI TCCGGA 1 cut(s) 822
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 3 cut(s) 231, 354, 439
BseGI GGATG 3 cut(s) 636, 721, 783
BseLI CCNNNNNNNGG 8 cut(s) 43, 64, 111, 206, 355, 379, 647, 749
BseMII CTCAG 2 cut(s) 489, 552
BseNI ACTGG 3 cut(s) 373, 658, 778
BsePI GCGCGC 1 cut(s) 561
BseRI GAGGAG 3 cut(s) 35, 134, 571
BseSI GKGCMC 2 cut(s) 485, 627
BseXI GCAGC 2 cut(s) 670, 776
BsgI GTGCAG 2 cut(s) 253, 783
Bsh1236I CGCG 4 cut(s) 401, 563, 565, 770
BshFI GGCC 6 cut(s) 180, 198, 216, 236, 353, 413
BshNI GGYRCC 3 cut(s) 67, 449, 509
BsiHKAI GWGCWC 2 cut(s) 627, 757
BsiSI CCGG 8 cut(s) 65, 195, 276, 302, 349, 393, 648, 823
BslI CCNNNNNNNGG 8 cut(s) 43, 64, 111, 206, 355, 379, 647, 749
BsmAI GTCTC 2 cut(s) 343, 466
BsnI GGCC 6 cut(s) 180, 198, 216, 236, 353, 413
Bso31I GGTCTC 1 cut(s) 343
Bsp1286I GDGCHC 4 cut(s) 118, 485, 627, 757
Bsp13I TCCGGA 1 cut(s) 822
Bsp143I GATC 4 cut(s) 249, 345, 501, 726
BspANI GGCC 6 cut(s) 180, 198, 216, 236, 353, 413
BspCNI CTCAG 2 cut(s) 490, 551
BspEI TCCGGA 1 cut(s) 822
BspFNI CGCG 4 cut(s) 401, 563, 565, 770
BspLI GGNNCC 7 cut(s) 24, 69, 199, 451, 511, 591, 742
BspPI GGATC 2 cut(s) 340, 734
BspQI GCTCTTC 1 cut(s) 80
BspT107I GGYRCC 3 cut(s) 67, 449, 509
BspTI CTTAAG 1 cut(s) 92
BspTNI GGTCTC 1 cut(s) 343
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 4 cut(s) 64, 194, 275, 392
BsrI ACTGG 3 cut(s) 373, 658, 778
BssAI RCCGGY 4 cut(s) 64, 194, 275, 392
BssECI CCNNGG 3 cut(s) 231, 354, 439
BssHII GCGCGC 1 cut(s) 561
BssMI GATC 4 cut(s) 249, 345, 501, 726
BssNI GRCGYC 2 cut(s) 68, 210
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 3 cut(s) 163, 172, 532
Bst6I CTCTTC 2 cut(s) 80, 152
BstACI GRCGYC 2 cut(s) 68, 210
BstAFI CTTAAG 1 cut(s) 92
BstBAI YACGTR 1 cut(s) 577
BstC8I GCNNGC 5 cut(s) 30, 66, 469, 563, 792
BstDEI CTNAG 2 cut(s) 498, 538
BstDSI CCRYGG 1 cut(s) 354
BstENI CCTNNNNNAGG 1 cut(s) 109
BstF5I GGATG 3 cut(s) 636, 721, 783
BstFNI CGCG 4 cut(s) 401, 563, 565, 770
BstH2I RGCGCY 3 cut(s) 71, 100, 498
BstHHI GCGC 6 cut(s) 70, 99, 403, 497, 563, 565
BstKTI GATC 4 cut(s) 252, 348, 504, 729
BstMAI GTCTC 2 cut(s) 343, 466
BstMBI GATC 4 cut(s) 249, 345, 501, 726
BstMWI GCNNNNNNNGC 7 cut(s) 70, 122, 222, 398, 494, 761, 806
BstNI CCWGG 1 cut(s) 232
BstSCI CCNGG 3 cut(s) 230, 300, 348
BstSFI CTRYAG 2 cut(s) 109, 472
BstSLI GKGCMC 2 cut(s) 485, 627
BstUI CGCG 4 cut(s) 401, 563, 565, 770
BstV1I GCAGC 2 cut(s) 670, 776
BstV2I GAAGAC 1 cut(s) 526
BstX2I RGATCY 1 cut(s) 726
BstYI RGATCY 1 cut(s) 726
BsuRI GGCC 6 cut(s) 180, 198, 216, 236, 353, 413
BtgI CCRYGG 1 cut(s) 354
BtgZI GCGATG 2 cut(s) 118, 211
BtsCI GGATG 3 cut(s) 636, 721, 783
BtsIMutI CAGTG 1 cut(s) 380
Cac8I GCNNGC 5 cut(s) 30, 66, 469, 563, 792
CfoI GCGC 6 cut(s) 70, 99, 403, 497, 563, 565
Cfr10I RCCGGY 4 cut(s) 64, 194, 275, 392
Cfr13I GGNCC 5 cut(s) 102, 197, 234, 351, 740
CseI GACGC 1 cut(s) 199
Csp6I GTAC 2 cut(s) 772, 781
CviAII CATG 2 cut(s) 247, 364
CviQI GTAC 2 cut(s) 772, 781
DdeI CTNAG 2 cut(s) 498, 538
DinI GGCGCC 1 cut(s) 69
DpnI GATC 4 cut(s) 251, 347, 503, 728
DpnII GATC 4 cut(s) 249, 345, 501, 726
DriI GACNNNNNGTC 1 cut(s) 746
EaeI YGGCCR 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 80, 152
Eam1105I GACNNNNNGTC 1 cut(s) 746
EarI CTCTTC 2 cut(s) 80, 152
Ecl136II GAGCTC 1 cut(s) 755
Eco147I AGGCCT 1 cut(s) 413
Eco24I GRGCYC 2 cut(s) 118, 757
Eco31I GGTCTC 1 cut(s) 343
Eco47I GGWCC 2 cut(s) 102, 740
Eco47III AGCGCT 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 755
Eco72I CACGTG 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 755
EcoNI CCTNNNNNAGG 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 230
EcoT38I GRGCYC 2 cut(s) 118, 757
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
FaeI CATG 2 cut(s) 250, 367
FaiI YATR 3 cut(s) 122, 248, 365
FatI CATG 2 cut(s) 246, 363
FblI GTMKAC 2 cut(s) 54, 525
Fnu4HI GCNGC 9 cut(s) 26, 71, 445, 448, 684, 765, 768, 795, 798
FokI GGATG 3 cut(s) 623, 728, 770
FriOI GRGCYC 2 cut(s) 118, 757
Fsp4HI GCNGC 9 cut(s) 26, 71, 445, 448, 684, 765, 768, 795, 798
GlaI GCGC 6 cut(s) 69, 98, 402, 496, 562, 564
GluI GCNGC 9 cut(s) 26, 71, 445, 448, 684, 765, 768, 795, 798
HaeII RGCGCY 3 cut(s) 71, 100, 498
HaeIII GGCC 6 cut(s) 180, 198, 216, 236, 353, 413
HapII CCGG 8 cut(s) 65, 195, 276, 302, 349, 393, 648, 823
HgaI GACGC 1 cut(s) 199
HhaI GCGC 6 cut(s) 70, 99, 403, 497, 563, 565
Hin1I GRCGYC 2 cut(s) 68, 210
Hin1II CATG 2 cut(s) 250, 367
Hin6I GCGC 6 cut(s) 68, 97, 401, 495, 561, 563
HinP1I GCGC 6 cut(s) 68, 97, 401, 495, 561, 563
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 807
HinfI GANTC 1 cut(s) 569
HpaII CCGG 8 cut(s) 65, 195, 276, 302, 349, 393, 648, 823
HphI GGTGA 3 cut(s) 151, 319, 637
Hpy166II GTNNAC 4 cut(s) 55, 265, 526, 625
Hpy188I TCNGA 4 cut(s) 345, 672, 740, 752
Hpy188III TCNNGA 3 cut(s) 418, 433, 823
Hpy8I GTNNAC 4 cut(s) 55, 265, 526, 625
Hpy99I CGWCG 5 cut(s) 56, 89, 167, 209, 215
HpyAV CCTTC 3 cut(s) 115, 227, 424
HpyCH4III ACNGT 3 cut(s) 163, 172, 532
HpyCH4IV ACGT 3 cut(s) 51, 156, 576
HpyCH4V TGCA 3 cut(s) 270, 625, 764
HpyF10VI GCNNNNNNNGC 7 cut(s) 70, 122, 222, 398, 494, 761, 806
HpyF3I CTNAG 2 cut(s) 498, 538
HpySE526I ACGT 3 cut(s) 51, 156, 576
Hsp92I GRCGYC 2 cut(s) 68, 210
Hsp92II CATG 2 cut(s) 250, 367
HspAI GCGC 6 cut(s) 68, 97, 401, 495, 561, 563
KasI GGCGCC 1 cut(s) 67
Kpn2I TCCGGA 1 cut(s) 822
KroI GCCGGC 1 cut(s) 64
KroNI GCCGGC 1 cut(s) 66
Kzo9I GATC 4 cut(s) 249, 345, 501, 726
LguI GCTCTTC 1 cut(s) 80
LmnI GCTCC 7 cut(s) 22, 383, 558, 595, 672, 752, 825
Lsp1109I GCAGC 2 cut(s) 670, 776
LweI GCATC 2 cut(s) 234, 666
MaeII ACGT 3 cut(s) 51, 156, 576
MaeIII GTNAC 4 cut(s) 157, 290, 307, 566
MalI GATC 4 cut(s) 251, 347, 503, 728
MbiI CCGCTC 1 cut(s) 73
MboI GATC 4 cut(s) 249, 345, 501, 726
MboII GAAGA 4 cut(s) 67, 139, 526, 721
MflI RGATCY 1 cut(s) 726
MhlI GDGCHC 4 cut(s) 118, 485, 627, 757
Mly113I GGCGCC 1 cut(s) 68
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 2 cut(s) 718, 730
MnlI CCTC 7 cut(s) 13, 152, 155, 161, 269, 449, 549
MreI CGCCGGCG 1 cut(s) 64
MroI TCCGGA 1 cut(s) 822
MroNI GCCGGC 1 cut(s) 64
MseI TTAA 2 cut(s) 93, 297
MslI CAYNNNNRTG 1 cut(s) 515
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 8 cut(s) 65, 195, 276, 302, 349, 393, 648, 823
MspR9I CCNGG 3 cut(s) 232, 302, 350
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 4 cut(s) 401, 563, 565, 770
MwoI GCNNNNNNNGC 7 cut(s) 70, 122, 222, 398, 494, 761, 806
NaeI GCCGGC 1 cut(s) 66
NarI GGCGCC 1 cut(s) 68
NciI CCSGG 2 cut(s) 302, 350
NdeII GATC 4 cut(s) 249, 345, 501, 726
NgoMIV GCCGGC 1 cut(s) 64
NlaIII CATG 2 cut(s) 250, 367
NlaIV GGNNCC 7 cut(s) 24, 69, 199, 451, 511, 591, 742
NmeAIII GCCGAG 4 cut(s) 151, 201, 420, 471
NmuCI GTSAC 4 cut(s) 157, 290, 307, 566
OliI CACNNNNGTG 1 cut(s) 515
PauI GCGCGC 1 cut(s) 561
PceI AGGCCT 1 cut(s) 413
PciSI GCTCTTC 1 cut(s) 80
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 684
PdiI GCCGGC 1 cut(s) 66
PflFI GACNNNGTC 2 cut(s) 50, 209
PflMI CCANNNNNTGG 1 cut(s) 379
PkrI GCNGC 9 cut(s) 27, 72, 446, 449, 685, 766, 769, 796, 799
PleI GAGTC 1 cut(s) 563
PluTI GGCGCC 1 cut(s) 71
PmaCI CACGTG 1 cut(s) 577
PmlI CACGTG 1 cut(s) 577
PpsI GAGTC 1 cut(s) 563
Ppu21I YACGTR 1 cut(s) 577
Psp124BI GAGCTC 1 cut(s) 757
Psp6I CCWGG 1 cut(s) 230
PspCI CACGTG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 7 cut(s) 24, 69, 199, 451, 511, 591, 742
PspPI GGNCC 5 cut(s) 102, 197, 234, 351, 740
PsuI RGATCY 1 cut(s) 726
PsyI GACNNNGTC 2 cut(s) 50, 209
PteI GCGCGC 1 cut(s) 561
RsaI GTAC 2 cut(s) 773, 782
RsaNI GTAC 2 cut(s) 772, 781
RseI CAYNNNNRTG 1 cut(s) 515
SacI GAGCTC 1 cut(s) 757
SalI GTCGAC 1 cut(s) 53
SapI GCTCTTC 1 cut(s) 80
SaqAI TTAA 2 cut(s) 93, 297
SatI GCNGC 9 cut(s) 26, 71, 445, 448, 684, 765, 768, 795, 798
Sau3AI GATC 4 cut(s) 249, 345, 501, 726
Sau96I GGNCC 5 cut(s) 102, 197, 234, 351, 740
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 3 cut(s) 232, 302, 350
SduI GDGCHC 4 cut(s) 118, 485, 627, 757
SfaNI GCATC 2 cut(s) 234, 666
SfcI CTRYAG 2 cut(s) 109, 472
SfoI GGCGCC 1 cut(s) 69
SgrAI CRCCGGYG 2 cut(s) 64, 392
SinI GGWCC 2 cut(s) 102, 740
SmiMI CAYNNNNRTG 1 cut(s) 515
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SseBI AGGCCT 1 cut(s) 413
SspDI GGCGCC 1 cut(s) 67
SstI GAGCTC 1 cut(s) 757
StuI AGGCCT 1 cut(s) 413
StyD4I CCNGG 3 cut(s) 230, 300, 348
TaaI ACNGT 3 cut(s) 163, 172, 532
TaiI ACGT 3 cut(s) 54, 159, 579
TaqI TCGA 3 cut(s) 54, 78, 84
TatI WGTACW 1 cut(s) 780
TauI GCSGC 7 cut(s) 28, 73, 447, 450, 770, 797, 800
Tru1I TTAA 2 cut(s) 93, 297
Tru9I TTAA 2 cut(s) 93, 297
TscAI CASTG 1 cut(s) 380
TseFI GTSAC 4 cut(s) 157, 290, 307, 566
TseI GCWGC 2 cut(s) 683, 764
Tsp45I GTSAC 4 cut(s) 157, 290, 307, 566
TspDTI ATGAA 2 cut(s) 121, 807
TspRI CASTG 1 cut(s) 380
Tth111I GACNNNGTC 2 cut(s) 50, 209
Van91I CCANNNNNTGG 1 cut(s) 379
Vha464I CTTAAG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 623
VpaK11BI GGWCC 2 cut(s) 102, 740
XagI CCTNNNNNAGG 1 cut(s) 109
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmiI GTMKAC 2 cut(s) 54, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.